CN1529812A - Electrophoretic apparatus - Google Patents

Abstract

An apparatus for performing a plurality of blots such as Western, Northern or Southern blots is disclosed in which each the blots are performed inside a container. The apparatus includes a multi-channel power supply comprising a common power supply supplying DC voltage in parallel to a plurality of computer controlled conversion modules. Each power conversion module includes a control means arranged to control the output of that module independently of the output of the other power conversion modules. The apparatus defines a housing arranged to receive a plurality of cassettes. Each cassette defining a pair of spaced apart electrodes and external contacts for supplying electric current of those electrodes. Each cassette is individually addressible by the apparatus such that a different voltage and current may be supplied to one cassette than to another cassette in the unit. The cassette has a thermally conductive base, an electrode depending into the container from the top of the cassette, and electrical contacts for supplying current to the lower electrode and upper electrode, said contacts being defined on the exterior to the cassette.

Description

Electrophoretic apparatus

Technical field

The present invention relates to be used to carry out the device of electrophoresis, be specifically related to a kind of trace system and device.

Background technology

Sometimes the blotting that is also referred to as electroblotting is a kind of known method, and the big molecule that will separate from gel by 1D or 2D electrophoretic process is transferred on the film from gel in the method.According to the big molecule type that carries out trace, such blotting is also called western part (Western) blotting, the north (Northern) blotting or south (Southern) blotting.Big molecule like this can comprise protein, peptide, carbohydrate, fat, nucleic acid molecules, comprise the compound bio molecule of glycoprotein, and their potpourri.Normally in polyacrylamide gel, carry out electrophoresis.

In known " semidry method " trace was handled, film was placed on the side of gel, and gel and film are clipped in two-layer filter paper or contain between the analog of damping fluid, more two-layer filter paper or the analog that contains damping fluid was clipped between anode and the negative electrode.The trace interlayer is exerted pressure, and electric current makes big molecule arrive on the film from gel by this interlayer.

Present known form with container is carried out electrophoresis, and container is the groove that is stamped lid, and an electrode is formed on the bottom of container further groove, is generally anode, and lid itself forms negative electrode, and the unit that comprises power supply is used to produce the electric field between anode and the negative electrode.

The existing trace system of even now can well move, but there are many defectives in their simplification and operating speed.

Simultaneously, such system only is applicable to the semidry method trace processing of carrying out containing damping fluid in filter paper, handles and be suitable for the wet method trace.

Also there is the problem that once can only carry out a trace usually in existing blotter.If device can carry out for example four or six traces simultaneously, will improve treatment capacity.Yet an importance of carrying out trace is the voltage and current that control is applied to this trace in operating process.This be since to trace apply excessive current/voltage may cause overheated, thereby the big molecule of broken ring.For example, when carrying out blotting, handle and need to give each trace that for example 250V and 250mA are provided for carrying out each trace.Like this, carry out two traces processing if walk abreast, then power supply provides 500mA's with 250V.Yet the feature of trace comprises the resistance of trace, is not identical.Like this, if two traces are handled parallel carrying out, a trace may remove more electric current than another branch.If a trace branch is walked too many electric current, it may be overheated and degraded, thereby destroyed biomolecule.Carry out two traces if only walk abreast and handle, one of two traces divide to walk the possibility of whole 500mA electric currents little.Thereby, carry out two traces processing if only walk abreast, the danger that damages trace is also not too high.Yet the parallel trace that carries out is many more, and the possibility of the high electric current that trace branch is walked to be difficult to bear is just big more, and this can increase the possibility of destroying this trace, and may damage the trace that other obtains too little current.

Thereby further purpose of the present invention provides and is applicable to and can handles the multiple power supplies that uses in the blotter of a plurality of gels simultaneously.

Any discussion that has comprised in this manual document, regulations, material, equipment, goods etc. only is used to provide background of the present invention.They exist in that Australia or other are local before date although in the right of priority of every claim of the application, but we do not think that part or all of these contents formed the prior art basis, or the common practise in the field relevant with the present invention.

Summary of the invention

In a broad sense, the invention provides a kind of device that carries out a plurality of traces, these traces for example are western, northern or southern trace, each trace carries out in container, wherein this device comprises multiple power supplies, this power supply is by providing the multiple power source of dc voltage to constitute to a plurality of computer controlled conversion, and wherein each power conversion module comprises that the output that is used to be independent of other power conversion module controls the control device of this module output.

Specifically, the invention provides a kind of parallel trace system that carries out a plurality of traces that is used for, this system comprises absorption carrier (blotter) unit that is set to hold a plurality of box bodys, each box body defines a pair of electrode that keeps at a certain distance away and is used for providing the outer contact of electric current to these electrodes, it is characterized in that each container by the independent addressing in unit, so that the voltage and current that is different from another container in the unit is provided for a container.

Preferably, this unit is defined as a plurality of box bodys and is positioned at wherein well or groove.The contacted contact of electric contact that is suitable for and is limited on one or more box bodys preferably is defined in the groove.

This unit typically has the hinged lid that is used in operation middle cover upper groove.

Preferably, grooved substrate is cooled off.This substrate generally includes the heating radiator that wherein has amber ear card array.Have certain space below the heating radiator, wherein be mounted with a series of fans that are used for cooling radiator.

By using different box bodys, this device/unit can be used for carrying out the electrophoresis of other type, as 1d or 2d electrophoresis.For example carry out 1d electrophoresis, 2d electrophoresis and trace operation successively.

Each container can be for having the box body form of box-like structure usually.The bottom of box body integral body or at least a portion is substantially formed by the not only good heat conductive of for example metal but also the material piece of good conductive, and it can play the effect of electrode like this, also can be from box body inside to heating radiator transmission heat.

The bottom of container typically is made of the steel disc that platinum covers the upper surface in container.The wall of box body and top can be made by molded plastic material.One wall of box body has hinge, and box body can switch and closed like this, thereby it is inner or therefrom remove to allow absorption carrier interlayer/lamination to be inserted into box body.Sealing can be set be the close form of liquid so that the upper and lower of box body lumps together substantially.Sealing can be realized by the O shape circle that extends around the box body bottom.

Box body can be disposable, and electrode is preferably made by the cheap electrode material of for example graphite in this case.To disposable box body device, except that O shape circle, other sealing can be set.

Second electrode descends outstanding inside to box body from box body top downside, and suspended by the bias unit of for example spring from the top.

Guide finger protrudes upward from the box body bottom, with help the absorption carrier interlayer in the box body is accurately located.

Possibility for avoiding the box body explosion that gas build caused that produces owing to electrolysis in box body or popping is provided with vent port on box body top.Retaining valve makes can only discharge gas from box body.

Box body has the outer contact that is electrically connected with the upper/lower electrode maintenance.

A special feature of the present invention is: the contact face of two electrodes makes progress and is positioned near the box body top.This has realized the more direct independent addressing of the electrode in the box body, and the contact making device that is provided with by the unit can be all outstanding down from lid, and electrode and unlike prior art, must be arranged in the lid and the bottom in.

Perhaps, the contact can be limited on the wall of groove.This makes provides electric energy than providing electric energy easier to lid to this contact.

This unit can comprise the removable guiding device that can be loaded in the unit further groove, thus the accurate location that can realize a plurality of box bodys in the groove, and like this when cap closure, the electric contact that limits in the contact in the lid and the box body is accurate locatees and contacts.Can provide different guiding devices for the box body that loads different size.

Like this, compared with prior art, system of the present invention is because can to carry out a plurality of traces simultaneously stacked, and each trace operation can also carry out under the condition that is different from other trace operation, thereby improved treatment capacity.For example, for each trace, the voltage that applies by the electrode in the independent box body, and the electric current that provides can be different with the duration.Current/voltage also can change in time.

Use box body to simplify Load System, and full-closed structure has been avoided the unit pollution.This makes this system can be used in half wet blotting.

This device typically comprises the multiple power supplies that is made of multiple power source, to the parallel dc voltage that provides of a plurality of computer controlled conversion.

Wherein, each power conversion module comprises control device and switching regulator transformer.

Wherein the switching regulator transformer comprise elementary winding, secondary winding and with the switchgear of elementary windings in series, be used for controlling the operation of power transformer in the line retrace mode, thereby when switchgear is ON, electric current increase in the elementary winding and the energy in the transformer increase, when switchgear is OFF, energy in the transformer is emitted as the electric current in time winding, and

Wherein control device is controlled the electric energy of transferring in the secondary winding from elementary winding by the load cycle of by-pass cock device.

Preferably, Computer Control Unit is connected on the power supply by optoisolator when work, and Computer Control Unit can be used to all output channels of control transformation module.

In a preferred embodiment, this device comprises the device that is used for monitoring secondary winding electric current and voltage output, if output voltage and/or electric current are exceeding one group of parameter preset, then passes through the load cycle of closed-loop control device by-pass cock device.Closed-loop control device typically comprises digital potentiometer or digital to analog converter (DAC).

The parameter preset group is typically defined by the user and be input in the Computer Control Unit.

In power supply, preferably combine overcurrent protection.

Preferably, power supply and consumption data be by the Computer Control Unit real-time collecting, and can store and the operating parameter of each test/operation of graphic presentation.

One preferable aspect, the current monitoring device that for example is connected to the resistance in series of voltage threshold detector is set to: if the electric current in the secondary winding surpasses preset maximum value, photo-coupler sends a signal to control circuit and comes temporary close switching regulator transformer.

Aspect alternative, the electric current of being told by secondary winding is connected to resistance on the constant temperature relaxation oscillator by an end, so that stream of pulses sends to photo-coupler, pulsation rate increases with the increase of output current, like this be arranged so that if the electric current in the secondary winding surpasses preset maximum value, photo-coupler sends signal with temporary close switching regulator transformer to control circuit.

Owing to used microprocessor to reset/the electric power monitor circuit, so relaxation oscillator or voltage-level detector are constant temperature.

Provide the box body that is used for system of the present invention in related fields of the present invention, having comprised:

Thermal-conductivity substrate;

The outstanding down electrode to container from the box body top; With

Be used for providing the electric contact of electric current to bottom electrode and top electrode, described electric contact is limited to the outside of box body.

The preferable feature of box body is limited by foregoing and following explanation.

Description of drawings

Below will be only by example and with reference to description of drawings specific embodiment of the present invention, in these figure:

Fig. 1 is the schematic, exploded of absorption carrier system, wherein shows two different box body options 5;

Fig. 2 is the sectional view through Fig. 1 absorption carrier unit, shows the box body that is arranged in the unit and the lid of closed cell;

Fig. 3 shows the box body that is used for unit shown in Figure 1;

Fig. 4 has shown the box body of the Fig. 3 that is in open mode;

Fig. 5 has shown the section through Fig. 3 box body;

Fig. 6 a is the circuit diagram that embodies the common segment of multiple power supplies of the present invention;

Fig. 6 b is the interface circuit figure that embodies power supply of the present invention and Computer Control Unit;

Fig. 6 c is the primary side circuit figure that embodies a path of multiple power supplies of the present invention;

Fig. 6 d is the out secondary circuit diagram that embodies a path of multiple power supplies of the present invention.

Embodiment

With reference to accompanying drawing, the absorption carrier unit form that Fig. 1 represents with common usefulness 10 shows a kind of device.The absorption carrier unit comprises housing 10, and this housing has been determined groove 12 and can be used to cover the also light hinged cover 14 of closed recess 12.Fig. 1 also shows guide frame 16, and this guide frame is suitable for being placed in the groove, and has determined six subframes 18, and the shape of each subframe and configuration are suitable for accepting box body 20.Fig. 1 also shows alternative shelf 22, when having only single relatively large box body 24 to pack in the groove, this shelf 22 is inserted in the groove.

Fig. 2 illustrates in greater detail the feature of unit/housing.The bottom that can see groove 12 has heating radiator 26, and this heating radiator generally includes has a series of planar metal sheet of outstanding heat radiator 28 down, and this heating radiator combines with cooling Peltier array 30.A space 32 that has held a plurality of electric fans 34 that are used for cooling radiator is arranged below the heating radiator.

This unit combines a multiple power supplies 36 that is used for providing power supply to the contact, and this power supply is by Computer Control Unit 40 controls.This power supply will describe in detail below.This unit is returned Peltier cooling array 30 power supply is provided.In addition, Computer Control Unit 40 controls offer the electric current and the voltage of the contact (not shown) that is positioned at lower lid surface.In alternative form, the contact is in the side of Peltier platform.The substrate 42 of unit is ventilated, come cooling radiator so that air flows through this unit.

In Fig. 3 to Fig. 5, can observe box body 20 better.Each box body is made up of the container that is generally box-like, and this container has a bottom 50, four sidewalls 52 and tops 54.Except the bottom, this container preferably forms with for example plastic material mold pressing of glass RPP.The bottom is made of the steel disc 56 that platinum covers, and this steel disc is not only as electrode, and as shifting the device of heats to chilled heating radiator 26 from being placed in absorption carrier lamination 58 on the electrode 56.Since platinum be inert material and not with the absorption carrier lamination in chemical reaction, so come the upper surface 56A of coated electrode with it.

As optimal viewing arrives among Fig. 4, Fig. 5, the rear wall 52A of box body is hinged, so that box body can open, thereby forms bottom 60 and top 62.O-ring seals 64 extends around the end face of box body bottom 60, makes that except the air hole 68 at box body top, box body is that liquid is close substantially when passing through intermediate plate 66 with the box body locking.

As Fig. 5 best image, top electrode is from outstanding in being suspended on two inside on the spring under the box body top.These springs have been guaranteed can apply appropriate pressure to interlayer 58 when absorption carrier interlayer 58 is inserted between two electrodes.

As Fig. 4 best image, a series of guide fingers 70 protrude upward from the box body bottom.These guide fingers participate in interlayer 58 is positioned at the center of bottom electrode 56.

With reference to Fig. 3, when box body was positioned at as shown in Figure 3 normal direction, the face of the contact 72,74 of upper/lower electrode upwards.Provide the electric contact of electric current to be limited on the lid 14 from the unit to each box body.Each group contact on the lid is controlled separately by CPU 40, when a plurality of box bodys for example shown in Figure 2 are arranged in the unit, can provide different voltage and currents for each box body like this.CPU 40 controls and monitoring offer the electric current and the voltage of single cartridge bodies, and can carry out the trace test under the specific electric current and voltage in one specific period by being programmed in, and close the power supply that offers single cartridge bodies when finishing at the fixed time then.

When carrying out a plurality of different traces operation at any time on the unit, the opening and closing of lid are cut to the power supply of each box body, and this is by the CPU record and carried out.This system can be used by a plurality of users simultaneously, and each user can carry out their trace operation separately.

In use, when power-off, from the unit of instrument, remove one or more box bodys.Box body is transferred to moist zone in testing laboratory, open and pack into successively: lower floor's absorption carrier interlayer (can from the kit that provides in advance), conventional sample, cellulose nitrate rete or PUDF or other suitable membrane material (this two-layer can insertion according to any order) and the upper strata absorption carrier folder layer that will handle.Absorption carrier damping fluid preimpregnation is to guarantee correct processing.Then box body is closed, this can be by using the spring-loaded system in each box body to apply suitable pressure to sample automatically.In the unit, put into single cartridge bodies or a plurality of box body then.Usually, the substrate of groove is covered by conduction oil, transmits with the heat that improves to heating radiator.The lid 14 of closing unit then.The operator selects the operator scheme with the CPU processing, and this operator scheme can be to be each unit predeterminated voltage, electric current and time, or more advanced setting, for example changes voltage in time.

In case the processing time end can be listened or visual detector can indicate the trace operation to stop.

Control system comprise to the FEEDBACK CONTROL of the temperature in testing.By thermoelectricity occasionally other suitable device measure Peltier temperature, give Computer Control Unit 40 with this information feedback, Computer Control Unit 40 reduces to supply with the voltage/current of trace under overheated situation.

The user opens the lid of instrument then, and single cartridge bodies or a plurality of box body are retracted humid area and testing laboratory again, box body can be opened herein and film taken off to be used for subsequent treatment.System allows the user that the electric current and voltage time and the thetagram of each unit are downloaded among the PC, so that carry out data storage or printing.

Box body can be made disposable box body, and wherein base plate 56 is made by the thin plastics that inside is coated with powdery graphite.

Fig. 6 a to Fig. 6 d shows the multiple power supplies that is used for providing electricity to the contact.Fig. 6 a show embody multiple power supplies of the present invention provide the multiple power source part of power supply jointly for six modular converters.Fig. 1 c and Fig. 1 d show the primary side and the out secondary of a modular converter respectively, and six such modular converters are arranged in a preferred embodiment.Each modular converter provides that have can be up to the power supply of 50W output.

From figure, the structure of power supply is apparent to those skilled in the art, thereby no longer describes the assembling of all elements and power supply hereinafter in detail.Following explanation mainly concentrates on the special and novel characteristics of the present invention.

At the common segment of power supply AC, supply with rectifier DB1 main current, this rectifier charges to 315V for holding capacitor C2.Parallel six modular converters that offer shown in Fig. 1 c and 1d of this dc voltage.The common segment of power supply also provides two low-voltages: offer the VR1 of photo-coupler and DCP with 6V DC, and offer the Vcc that control circuit SG3524 and U7 cmos buffer device use with 8V DC.

Each modular converter combines the iron core switching type power supply transformer TR3 with the flyback mode operation as mentioned below.It comprises the iron core 104 with 11mm diameter.The iron core type is ETD34.The elementary winding that is wrapped on the bobbin around iron core is that the metal wire of 0.45mm constitutes by 200 circle diameters.Around the secondary winding in the elementary winding outside is that the metal wire of 0.45mm constitutes by 200 circle diameters also.Between elementary winding and secondary winding, be provided with insulator.300 microns clearance is provided with suitable inductance and allows energy storage.

In the switching type power supply transformer, secondary winding separates with elementary winding.The voltage ON/OFF pulse 82 of 315V periodically offers elementary winding 106, and when pulse was ON, this winding increased the energy that is stored in the iron core.And the MOSFET Q2 of elementary windings in series switches pulse between ON and OFF.When switch was ON, the electric current in the winding increased by MOSFET Q2 and resistance R 31A.When switch was OFF, the electric current in the iron core winding discharged in the capacitor C 18 by diode D3.Electric current flows through in secondary circuit, makes capacitor C 18 chargings, and this electric capacity next inductance L through having removed radio-frequency component 1 gives capacitor C 19 chargings.Except when pulse is when being OFF, the polarity of diode D3 prevents that electric current from flowing through in secondary.

By using switching type power supply, can be by changing the load cycle that elementary pulse is the elementary winding cycle of ON, change from elementary and be sent to secondary electric energy.

In order to obtain specific output voltage, control offers the electric energy of secondary circuit.As the part of control and treatment, obtain the sampling of voltage in during OFF.At elementary sampling secondary voltage, and ground is called V _FeedbackCapacitor C 14 is charged to this voltage, and electric current flows through variable resistor R53 then.The electric current that flows through R53 produces and the proportional voltage of output voltage.The reference voltage of 2V is provided for control circuit SG3524.In use, adjust the voltage cycle of supplying with the switching regulator primary, make output voltage equal reference voltage, just offer the 2.0V of IN+.Like this, reduce feedback voltage rather than adjust reference voltage IN+ with the rough pot 130 that combines R53.The wide ranges (above 4: 1) of this feasible output voltage that is provided, and the common mode voltage of the reference signal IN+ that does not change at SG3524.Another pot 140 is set carries out minute adjustment, so that increase to 11 comprehensively from the resolution of 6 of every pots.

Use another pot 150 to send the CUTOFF instruction.At the ON state, pot 150 provides 0V output.When needs CUTOFF, provide 6V output.

In modular converter, there are many security features.If the electric current in the transformer is too high, this will reflect with the voltage increase by R31A, and the R31A here provides the control voltage that is input among the control circuit SG3524 and temporarily control circuit U4 output closed.If it is the preset maximum value of 250V, then temporary close power supply in described embodiment that voltage surpasses.

In secondary output current monitoring resistance is set.Current feedback transmits electric current and gives photo-coupler U6.If the electric current in the resistance R 42 of secondary circuit output terminal surpasses 0.3amps, the electric current among the photo-coupler U6 sends a signal to the control circuit SG3524 of closing control chip and transformer.

Owing to used microprocessor reset/power supply supervisory circuit, current threshold detector U8 is a constant temperature.

As a safety practice, should note the primary and secondary circuit of module is separated fully, will increase operator's security, the influence that the operator can not be got an electric shock greatly like this.

Fig. 1 b shows the control bus interface of Computer Control Unit.Photo-coupler E11, E12 and E13 are directly connected on the main line control circuit.Sda line has two functions: transmit and receive data, and this is as high impedance.Input has the 50ohms Low ESR with general concentric conductor compatibility.

U13 provides clock signal by another photo-coupler.This system uses known 12C agreement, is divided into two lines that separate so that impedance remains on relatively low 50ohms except carrying with the reception line.

Electric current and voltage are monitored by separation module, and this module also is connected on the same Computer Control Unit 106.

This data storing and provides software to come by the graphic presentation result in Computer Control Unit 40.

If necessary, for example when the more large-area trace test of operation box body, this equipment allows several passages are connected across together, so that produce higher wattage.

A specific characteristic of power supply of the present invention is: from multichannel output voltage is negative.If necessary, this casing that can make the equipment such as electrophoretic apparatus that contain power supply is a positive terminal.

This system combines a temperature sensor.Its periodicity and temporary connection make the 2mA fixed current flow through temperature-dependent resistance.Measure voltage and determine temperature with this.Temporary transient this resistance of connecting has been avoided current heating resistor.

Compare with existing electrophoresis power, above-mentioned multiple power supplies has many substantial advantage.Specifically, can realize computer control is carried out in each path/output separately.Optoisolator makes this device to carry out computer control by mouse pad (mouse pad)/graphic presentation interface, and the complete light of these equipment and power supply is isolated, and has guaranteed computer control and operator's safety.

Use switching type power supply to make this device relative compact.The switching regulator transformer efficiency is very high, and less relatively as the energy of heat dissipation.Since to the temperature of on such device, carrying out various tests must accurately control and overheated may failure test, so this point particular importance for the device that carries out electrophoresis.

By the voltage and current parameter of monitoring from each unit/output, multiple power supplies also provides the control to independent path, can adjust parameter through closed loop then, thereby guarantees to finish electrophoresis in the parameter area that the user is provided with.

Can make power supply very compact by use switching regulator transformer, even can be used as a multichannel unit, and can make power supply that 50 to 200V wide-range voltage is provided with high-level efficiency.This compactness can make power supply be attached in the casing of electrophoretic apparatus, rather than exists as independent parts.With regard to its occupied testing laboratory space, this has guaranteed that not only the shared area of this device is an acceptable, and, mean that also the people such as researchist of operation gel on equipment needn't worry to be a plurality of power supply lines because this device is compressed in the equipment.

The present invention also makes Computer Control Unit 40 can collect power supply/consumption data and the operating parameter of each output/unit that presents in diagrammatic form in real time.This can make the people who tests store the electrophoretic process data for each trace.

Another feature of the present invention is that single Computer Control Unit 40 can be used for controlling whole six output channels.

A special benefits of the present invention is: because all wirings all are installed in the device, can not consider to relate to the safety problem of using this device near moist environment.

Though above-mentioned specific embodiment provides six independent controlled outputs, also can use more or less output channel.

And though the switching regulator transformer has 200 circles at the primary and secondary winding, those skilled in the art know that also the number of turn is variable.For example can 400 circles be set, thereby output voltage is doubled to 400V from 200V at secondary winding.

And, by the simple modification of secondary circuit, particularly add voltage multiplier, can provide higher voltage (surpassing 1kV) for more high-tension technology of needs such as for example isoelectric focusing or many compartments electrolysis.

Those skilled in the art should be known in and can carry out multiple change and/or improvement to the present invention shown in the specific embodiment, and do not break away from the spirit and scope of the present invention of general description.Therefore, should regard these embodiment as illustrative and not restrictive in all respects.

Claims (37)

1. device that is used in container, carrying out a plurality of traces, these traces for example are western, northern or southern trace, wherein this device comprises one by the multiple power supplies that constitutes to the parallel multiple power source that DC voltage is provided of a plurality of computer controlled conversion, and wherein each power conversion module comprises that an output that is used for being independent of other power conversion module controls the control device of the output of this module.

2. device as claimed in claim 1, wherein container is the box body form, this device has determined to be used to hold the housing of a plurality of box bodys, each box body has a pair of electrode separated by a distance and the outer contact that is used for providing electric current to those electrodes, each box body installs independent addressing by this, so that provide the voltage and current that is different from another box body in the unit to a box body.

3. device as claimed in claim 2, this device define the well or the groove that wherein can hold a plurality of box bodys.

4. device as claimed in claim 3, wherein be suitable for be limited to one or more box bodys on the outer contact that contacts of electric contact be limited in the well or in the groove.

5. as claim 3 or 4 described devices, wherein this device comprises the hinged lid that is used for closed recess when operation.

6. any as described above described device of claim, wherein this container is positioned on the substrate that is limited by housing in use, and this substrate comprises the heating radiator with Bohr's card array.

7. device as claimed in claim 6 wherein provides a series of fans that are used for cooling radiator in the space below heating radiator.

8. any as described above described device of claim, wherein this container comprises the box body with box-like structure, for example the good heat conductive of metal and the material of conduction constitute by a slice to small part in the bottom of box body, the box body bottom has the effect of electrode like this, and can be from the inner heat of deriving of box body.

9. device as claimed in claim 8, wherein the bottom of this container comprises that the upper surface towards internal tank is covered with the steel disc of platinum.

10. install as claimed in claim 8 or 9, wherein this box body has the wall and the top of being made by molded plastic material, and wherein box body wall has hinge, so that can open or close box body, thus allow the absorption carrier interlayer is inserted into box body inside.

11. device as claimed in claim 10 wherein is provided with O shape O-ring between the top and the bottom of box body, so that the top and the bottom of box body are closed together with the close form of abundant liquid.

12. as any described device in the claim 8 to 11, wherein the bottom of box body is made of graphite.

13. as any described device in the claim 8 to 12, wherein electrode is outstanding in down box body inside from the downside at box body top, and is suspended on for example top of the polarising means of spring.

14. as any described device in the claim 8 to 13, wherein guide finger protrudes upward from the box body bottom, with realization the absorption carrier interlayer in the box body is accurately located.

15. as any described device in the claim 8 to 14, wherein on box body top vent port is set, wherein retaining valve only allows gas to discharge box body.

16. as any described device in the claim 8 to 15, wherein the contact of two electrodes in the box body all makes progress, and is positioned near the box body top.

17. device as claimed in claim 16 is wherein outstanding down from lid by the contact that this device provides.

18. as any described device in the claim 8 to 15, wherein the contact that is provided by this device is positioned on the wall of groove.

19. device as claimed in claim 17, further comprise the removable guiding device that can be positioned at the housing further groove, be used for realizing the accurate location of a plurality of box bodys at groove, so that when cap closure, the contact in the lid is accurately located with the electric contact that limits by box body and is contacted.

20. any as described above described device of claim, wherein:

Each power conversion module comprises control device and switching regulator transformer,

Wherein the switching regulator transformer comprise elementary winding, secondary winding and with the switchgear of elementary windings in series, be used for controlling the operation of power transformer in the flyback mode, thereby when switchgear when opening, electric current increase in the elementary winding and the energy in the transformer increase, when switchgear is the pass, energy in the transformer emit as the electric current in the secondary winding and

Wherein control device is controlled the electric energy of transferring to secondary winding from elementary winding by the load cycle of by-pass cock device.

21. multiple power supplies as claimed in claim 20 further comprises by optoisolator being connected on the power supply and being used for the Computer Control Unit of all output channels of control transformation module.

22. as claim 20 or 21 described multiple power supplies, comprise the device that is used to monitor secondary winding electric current and voltage output, and if output voltage and/or electric current exceeded default parameter group, by the load cycle of closed-loop control device by-pass cock device.

23. multiple power supplies as claimed in claim 22, wherein closed-loop control device comprises digital potentiometer.

24. multiple power supplies as claimed in claim 22, wherein closed-loop control system comprises digital to analog converter.

25. as any described multiple power supplies in the claim 22 to 24, wherein this group parameter preset is defined by the user and be input in the Computer Control Unit.

26. as any described multiple power supplies in the claim 21 to 25, wherein by Computer Control Unit real-time collecting power supply and consumption data, with storage and the graphical operating parameter that shows each test/operation.

27. as any described multiple power supplies in the claim 20 to 26, the current monitoring device of the resistance in series that for example is connected to voltage threshold detector wherein is provided, if so that the electric current in the secondary winding surpasses preset maximum value, photo-coupler sends signal with temporarily disconnected switching regulator transformer to control device.

28. as any described multiple power supplies in the claim 20 to 26, the electric current of wherein telling from the secondary winding end of flowing through is connected to resistance on the constant temperature relaxation oscillator, so that stream of pulses is sent to photo-coupler, pulsation rate increases with the increase of output current, if the electric current in the secondary winding surpasses preset maximum value like this, photo-coupler sends to control device with the temporarily disconnected transformer with signal.

29. a box body that is used in any one device of claim 1 to 7, this box body comprises:

The bottom of heat conduction;

The outstanding down electrode in container from the box body top; With

Be used for electric current is offered the electric contact of bottom electrode and top electrode, described contact is limited on the box body outside.

30. box body as claimed in claim, wherein the bottom of container comprises that the upper surface towards internal tank is covered with the steel disc of platinum.

31. as claim 29 or 30 described box bodys, wherein this box body has the wall and the top of being made by molded plastic material, wherein box body wall has hinge, so that can open or close box body, thus allow the absorption carrier interlayer is inserted into box body inside.

32. box body as claimed in claim 31 wherein is provided with O shape O-ring between the top and the bottom of box body, so that the top and the bottom of box body are closed together with the close form of abundant liquid.

33. box body as claimed in claim 29, wherein the bottom of box body includes graphite.

34. as any described box body in the claim 29 to 33, wherein bridged electrode is suspended on the top of the polarising means of a spring for example or a plurality of springs.

35. as any described box body in the claim 29 to 34, wherein guide finger protrudes upward from the box body bottom, with realization the absorption carrier interlayer in the box body is accurately located.

36. as any described box body in the claim 29 to 35, wherein on box body top vent port is set, wherein retaining valve only allows gas to discharge box body.

37. as any described box body in the claim 29 to 36, wherein the contact of two electrodes in the box body all makes progress, and is positioned near the box body top.

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