CN1513988A - Production method of corn superoxide dismutase multienzyme - Google Patents
Production method of corn superoxide dismutase multienzyme Download PDFInfo
- Publication number
- CN1513988A CN1513988A CNA031338755A CN03133875A CN1513988A CN 1513988 A CN1513988 A CN 1513988A CN A031338755 A CNA031338755 A CN A031338755A CN 03133875 A CN03133875 A CN 03133875A CN 1513988 A CN1513988 A CN 1513988A
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- China
- Prior art keywords
- corn
- multienzyme
- superoxide dismutase
- production method
- filtrate
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 240000008042 Zea mays Species 0.000 title claims abstract description 109
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title claims abstract description 109
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 109
- 235000005822 corn Nutrition 0.000 title claims abstract description 109
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 17
- 102000019197 Superoxide Dismutase Human genes 0.000 title claims description 33
- 108010012715 Superoxide dismutase Proteins 0.000 title claims description 33
- 239000002994 raw material Substances 0.000 claims abstract description 16
- 238000001914 filtration Methods 0.000 claims abstract description 12
- 239000012535 impurity Substances 0.000 claims abstract description 11
- 238000001035 drying Methods 0.000 claims abstract description 10
- 238000002791 soaking Methods 0.000 claims abstract description 6
- 239000000706 filtrate Substances 0.000 claims description 30
- 239000007788 liquid Substances 0.000 claims description 30
- 230000035784 germination Effects 0.000 claims description 20
- 239000003963 antioxidant agent Substances 0.000 claims description 15
- 230000003078 antioxidant effect Effects 0.000 claims description 15
- 239000012528 membrane Substances 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 12
- 102000004190 Enzymes Human genes 0.000 claims description 10
- 108090000790 Enzymes Proteins 0.000 claims description 10
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 7
- 229910000397 disodium phosphate Inorganic materials 0.000 claims description 7
- 235000019800 disodium phosphate Nutrition 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 239000001488 sodium phosphate Substances 0.000 claims description 7
- 230000004913 activation Effects 0.000 claims description 5
- 229920002678 cellulose Polymers 0.000 claims description 5
- 239000001913 cellulose Substances 0.000 claims description 5
- 238000004890 malting Methods 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 239000008399 tap water Substances 0.000 claims description 5
- 235000020679 tap water Nutrition 0.000 claims description 5
- 238000000108 ultra-filtration Methods 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 238000007654 immersion Methods 0.000 claims description 4
- 229920002472 Starch Polymers 0.000 claims description 3
- 244000269722 Thea sinensis Species 0.000 claims description 3
- 239000000919 ceramic Substances 0.000 claims description 3
- 150000004676 glycans Chemical class 0.000 claims description 3
- 239000002184 metal Substances 0.000 claims description 3
- 229920001282 polysaccharide Polymers 0.000 claims description 3
- 239000005017 polysaccharide Substances 0.000 claims description 3
- 238000011084 recovery Methods 0.000 claims description 3
- 239000008107 starch Substances 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 238000001291 vacuum drying Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 5
- 241000894006 Bacteria Species 0.000 abstract 1
- 230000003213 activating effect Effects 0.000 abstract 1
- 238000004537 pulping Methods 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 description 9
- 239000000047 product Substances 0.000 description 4
- 229940032362 superoxide dismutase Drugs 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
A process for preparing corn SOD multienzyme includes providing raw materials, removing impurities, soaking activating, germinating, pulping, filtering for removing bacteria, dewatering and drying. Its advantages are low cost and high activity and thermal stability.
Description
Technical field
The present invention relates to the production method of superoxide-dismutase multienzyme, exactly is the production method of corn superoxide dismutase from corn multienzyme.
Background technology
The superoxide-dismutase multienzyme is meant superoxide-dismutase and Selenoperoxidase, catalatic mixture.This mixture is effectively to remove superoxide anion in the body---free radical to the main effect of human body, reduces cells in vivo damage or dead, thereby reduces the disease generation and delay senility.The method of existing production superoxide dismutase multienzyme mainly contains two classes, and a class is to extract from pluck, and another kind of is to extract from plants such as corn.In the drying products process, this method has adopted lyophilize or spraying. dry technology, and the former cost is higher, and the loss alive of latter's enzyme is bigger, and both all are not suitable for the needs of scale operation.
Summary of the invention
The objective of the invention is to provide a kind of production method of corn superoxide dismutase from corn multienzyme for people, so that produce in the described multienzyme process in this method of application, can reduce cost of goods manifactured, reduce enzyme loss alive, thereby overcome the problems referred to above that prior art exists.
The production method of corn superoxide dismutase from corn multienzyme provided by the invention is made up of following steps:
A, get the raw materials ready
Get an amount of raw material corn ready;
B, removal of impurities, immersion
Remove impurity in the raw material corn, this corn is inserted in the round-robin tap water soak again, soak time is 3-8 hour, takes out standby then;
C, activation, germination
It is that 0.0001-0.0003%, temperature are to soak in 15-30 ℃ the red enzyme cellulose solution that corn after will soaking through process B is inserted concentration, soak time is 2-10 hour, taking out corn then and it is inserted room temp is in 20-30 ℃ the malting floor, naturally deposited 15-24 hour, make it to germinate, take out standby thereafter;
D, slurrying
Adding concentration in the germination corn is the disodium phosphate soln of 0.08-0.15%, and to make the ratio of weight and number of germination corn and disodium phosphate soln be 1: 2-4, utilize disintegrating apparatus that germination corn and disodium phosphate soln powder mix are broken into corn steep liquor again;
E, filtration sterilization
With described corn steep liquor stir the companion evenly after, filter with first filter, remove filter residue, collect filtrate just, continue and with filter membrane first filtrate carried out essence and filter, and remove filter residue, collect smart filtrate; Smart filtrate is passed through in molecular weight is the ultrafiltration system of 15-30 ten thousand, remove filter residue again, collect final filtrate and add antioxidant therein, the add-on of this antioxidant is the 0.01-0.8% of final filtrate weight, makes sterile liquid thus.
F, removal moisture content
Make water evaporates in the described sterile liquid by vacuum-evaporator, regather remaining liquid, this liquid becomes corn superoxide dismutase from corn multienzyme liquid;
G, drying
With described corn superoxide dismutase from corn multienzyme liquid with insert in the loft drier through the abundant mixing of active carrier modified, after loose, under 40-90 ℃ of condition dry 1-6 hour, promptly make corn superoxide dismutase from corn multienzyme pulvis.
Above-mentioned antioxidant is the tea Dove.
Above-mentioned antioxidant is VC.
Above-mentioned active carrier is a starch.
Above-mentioned active carrier is a polysaccharide.
Above-mentioned loft drier is a vacuum drying oven.
Above-mentioned loft drier is common loft drier.
Above-mentioned filter membrane is a metal filtration film.
Above-mentioned filter membrane is a ceramic filtration membrane.
The present invention compared with prior art has following advantage after realizing: in the product drying process, because the present invention is drying plant with the loft drier, thereby can not only reduces drying cost significantly and can effectively improve enzyme activity.Test shows, when storing product of the present invention under 45 ℃ of conditions, its enzyme activity was not seen reduction in 60 days, and when storing product of the present invention at ambient temperature, its enzyme activity was not seen reduction in 24 months.
Embodiment
Embodiment one
A, get the raw materials ready
Get raw material corn 300kg ready;
B, removal of impurities, immersion
Remove impurity in the raw material corn, this corn is inserted in the round-robin tap water soak again, soak time is 8 hours, takes out standby then;
C, activation, germination
Corn after will soaking through process B inserts that concentration is 0.0001%, temperature is to soak in 30 ℃ the red enzyme cellulose solution, soak time is 2 hours, and taking out corn then and it is inserted room temp is in 30 ℃ the malting floor, deposits naturally 15 hours, make it to germinate, take out standby thereafter;
D, slurrying
In the germination corn, add concentration and be 0.11% disodium phosphate soln, and to make the ratio of weight and number of germination corn and above-mentioned solution be 1: 4, utilize pulverizer that germination corn and above-mentioned solution powder mix are broken into corn steep liquor again;
E, filtration sterilization
With described corn steep liquor stir the companion evenly after, filter with first filter, remove filter residue, collect filtrate just, continue and with filter membrane first filtrate carried out essence and filter, and remove filter residue, collect smart filtrate; Smart filtrate is passed through in molecular weight is the ultrafiltration system of 15-30 ten thousand, remove filter residue again, collect final filtrate and add antioxidant therein, the add-on of this antioxidant is 0.08% of a final filtrate weight, makes sterile liquid thus.
F, removal moisture content
Make water evaporates in the described sterile liquid by vacuum-evaporator, regather remaining liquid, this liquid becomes corn superoxide dismutase from corn multienzyme liquid;
G, drying
With described corn superoxide dismutase from corn multienzyme liquid with insert in the loft drier through the abundant mixing of active carrier modified, after loose, under 60 ℃ of conditions dry 6 hours, promptly make corn superoxide dismutase from corn multienzyme pulvis.
Embodiment two
A, get the raw materials ready
Get raw material corn 300kg ready;
B, removal of impurities, immersion
Remove impurity in the raw material corn, corn is immersed in the round-robin tap water soak again, soak time is 3 hours, takes out standby then;
C, activation, germination
Corn after will soaking through process B inserts that concentration is 0.0003%, temperature is to soak in 26 ℃ the red enzyme cellulose solution, soak time is 2 hours, and taking out corn then and it is inserted room temp is in 26 ℃ the malting floor, deposits naturally 24 hours, make it to germinate, take out standby thereafter;
D, slurrying
In the germination corn, add concentration and be 0.097% disodium phosphate soln, and to make the ratio of weight and number of germination corn and above-mentioned solution be 1: 2, utilize pulverizer that germination corn and above-mentioned solution powder mix are broken into corn steep liquor again, take out standby then;
E, filtration sterilization
With described corn steep liquor stir the companion evenly after, filter with first filter, remove filter residue, collect filtrate just, continue and with filter membrane first filtrate carried out essence and filter, and remove filter residue, collect smart filtrate; Smart filtrate is passed through in molecular weight is the ultrafiltration system of 15-30 ten thousand, remove filter residue again, collect final filtrate and add antioxidant therein, the add-on of this antioxidant is 0.8% of a final filtrate weight, makes sterile liquid thus.
F, recovery ethanol
Make water evaporates in the described sterile liquid by vacuum-evaporator, regather remaining liquid, this liquid becomes corn superoxide dismutase from corn multienzyme liquid;
G, drying
Will described corn superoxide dismutase from corn multienzyme liquid with through inserting in the loft drier behind the abundant mixing of modifying of active carrier, under 80 ℃ of conditions dry 5 hours, promptly make corn superoxide dismutase from corn multienzyme pulvis.
Embodiment three
A, get the raw materials ready
Get raw material corn 300kg ready;
B, removal of impurities
Remove impurity in the raw material corn, corn is inserted in the round-robin tap water soak again, soak time is 5 hours, takes out standby then;
C, activation, germination
Corn after will soaking through process B inserts that concentration is 0.0002%, temperature is to soak in 23 ℃ the red enzyme cellulose solution, soak time is 6 hours, and taking out corn then and it is inserted room temp is in 25 ℃ the malting floor, deposits naturally 20 hours, make it to germinate, take out standby thereafter;
D, slurrying
In the germination corn, add concentration and be 0.1% disodium phosphate soln, and to make the ratio of weight and number of germination corn and above-mentioned solution be 1: 3, utilize pulverizer that germination corn and above-mentioned solution powder mix are broken into corn steep liquor again, take out standby then;
E, filtration sterilization
With described corn steep liquor stir the companion evenly after, filter with first filter, remove filter residue, collect filtrate just, continue and with filter membrane first filtrate carried out essence and filter, and remove filter residue, collect smart filtrate; Smart filtrate is passed through in molecular weight is the ultrafiltration system of 15-30 ten thousand, remove filter residue again, collect final filtrate and add antioxidant therein, the add-on of this antioxidant is 0.4% of a final filtrate weight, makes sterile liquid thus.
F, removal moisture content
Make water evaporates in the described sterile liquid by vacuum-evaporator, and with its recovery, in order to reusing, regather remaining liquid, this liquid becomes corn superoxide dismutase from corn multienzyme liquid;
G, drying
With described corn superoxide dismutase from corn multienzyme liquid with insert in the loft drier through the abundant mixing of active carrier modified, after loose, under 70 ℃ of conditions dry 4 hours, promptly make corn superoxide dismutase from corn multienzyme pulvis.
Should illustrate that in implementing process of the present invention, described antioxidant promptly can be that the tea Dove also can be VC; Described active carrier promptly can be a starch, also can be polysaccharide; Described loft drier promptly can be a vacuum drying oven, also can be common loft drier; Described filter membrane promptly can be a metal filtration film, also can be ceramic filtration membrane.
Claims (9)
1, the production method of corn superoxide dismutase from corn multienzyme is characterized in that it is made up of following process:
A, get the raw materials ready
Get an amount of raw material corn ready;
B, removal of impurities, immersion
Remove impurity in the raw material corn, this corn is inserted in the round-robin tap water soak again, soak time is 3-8 hour, takes out standby then;
C, activation, germination
It is that 0.0001-0.0003%, temperature are to soak in 15-30 ℃ the red enzyme cellulose solution that corn after will soaking through process B is inserted concentration, soak time is 2-10 hour, taking out corn then and it is inserted room temp is in 20-30 ℃ the malting floor, naturally deposited 15-24 hour, make it to germinate, take out standby thereafter;
D, slurrying
Adding concentration in the germination corn is the disodium phosphate soln of 0.08-0.15%, and to make the ratio of weight and number of germination corn and above-mentioned solution be 1: 2-4, utilize disintegrating apparatus that germination corn and above-mentioned solution powder mix are broken into corn steep liquor again;
E, filtration sterilization
With described corn steep liquor stir the companion evenly after, filter with first filter, remove filter residue, collect filtrate just, continue and with filter membrane first filtrate carried out essence and filter, and remove filter residue, collect smart filtrate; Smart filtrate is passed through in molecular weight is the ultrafiltration system of 15-30 ten thousand, remove filter residue again, collect final filtrate and add antioxidant therein, the add-on of this antioxidant is the 0.01-0.8% of final filtrate weight, makes sterile liquid thus;
F, removal moisture content
Make water evaporates in the described sterile liquid by vacuum-evaporator, and with its recovery, in order to reusing, regather remaining liquid, this liquid becomes corn superoxide dismutase from corn multienzyme liquid;
G, drying
With described corn superoxide dismutase from corn multienzyme liquid with insert in the loft drier through the abundant mixing of active carrier modified, after loose, under 40-90 ℃ of condition dry 1-6 hour, promptly make corn superoxide dismutase from corn multienzyme pulvis.
2, the production method of corn superoxide dismutase from corn multienzyme according to claim 1 is characterized in that described antioxidant is the tea Dove.
3, the production method of corn superoxide dismutase from corn multienzyme according to claim 1 is characterized in that described antioxidant is VC.
4, the production method of corn superoxide dismutase from corn multienzyme according to claim 1 is characterized in that described active carrier is a starch.
5, the production method of corn superoxide dismutase from corn multienzyme according to claim 1 is characterized in that described active carrier is a polysaccharide.
6, the production method of corn superoxide dismutase from corn multienzyme according to claim 1 is characterized in that described loft drier is a vacuum drying oven.
7, the production method of corn superoxide dismutase from corn multienzyme according to claim 1 is characterized in that described loft drier is common loft drier.
8, the production method of corn superoxide dismutase from corn multienzyme according to claim 1 is characterized in that described filter membrane is a metal filtration film.
9, the production method of corn superoxide dismutase from corn multienzyme according to claim 1 is characterized in that described filter membrane is a ceramic filtration membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03133875 CN1234853C (en) | 2003-07-09 | 2003-07-09 | Production method of corn superoxide dismutase multienzyme |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03133875 CN1234853C (en) | 2003-07-09 | 2003-07-09 | Production method of corn superoxide dismutase multienzyme |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1513988A true CN1513988A (en) | 2004-07-21 |
| CN1234853C CN1234853C (en) | 2006-01-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03133875 Expired - Lifetime CN1234853C (en) | 2003-07-09 | 2003-07-09 | Production method of corn superoxide dismutase multienzyme |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101892204A (en) * | 2010-06-18 | 2010-11-24 | 李作红 | Method for preparing corn superoxide dismutase from corn |
| CN103223161A (en) * | 2013-05-13 | 2013-07-31 | 甘肃省人民医院 | Application of manganese superoxide dismutase stimulant in preparing liver-protecting drug |
| CN111041007A (en) * | 2019-12-04 | 2020-04-21 | 上海润日生物科技发展有限公司 | Preparation method of superoxide dismutase |
| CN113373122A (en) * | 2021-07-29 | 2021-09-10 | 辽宁未来生物科技有限公司 | Preparation method of novel corn superoxide dismutase multienzyme |
| WO2022068615A1 (en) * | 2020-09-29 | 2022-04-07 | 中国农业大学 | Formulation for improving activity and/or thermostability of superoxide dismutases and use thereof |
-
2003
- 2003-07-09 CN CN 03133875 patent/CN1234853C/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101892204A (en) * | 2010-06-18 | 2010-11-24 | 李作红 | Method for preparing corn superoxide dismutase from corn |
| CN103223161A (en) * | 2013-05-13 | 2013-07-31 | 甘肃省人民医院 | Application of manganese superoxide dismutase stimulant in preparing liver-protecting drug |
| CN111041007A (en) * | 2019-12-04 | 2020-04-21 | 上海润日生物科技发展有限公司 | Preparation method of superoxide dismutase |
| WO2022068615A1 (en) * | 2020-09-29 | 2022-04-07 | 中国农业大学 | Formulation for improving activity and/or thermostability of superoxide dismutases and use thereof |
| CN113373122A (en) * | 2021-07-29 | 2021-09-10 | 辽宁未来生物科技有限公司 | Preparation method of novel corn superoxide dismutase multienzyme |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1234853C (en) | 2006-01-04 |
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Owner name: LAIFU HEALTH BIOENGINEERING CO., LTD., ECONOMIC AN Free format text: FORMER OWNER: WANG JIANYING Effective date: 20110127 Owner name: LIAONING CHENGDA HEALTH FOOD CO., LTD. Free format text: FORMER OWNER: LAIFU HEALTH BIOENGINEERING CO., LTD., ECONOMIC AND TECHNOLOGICAL DEVELOPMENT AREA, DALIAN Effective date: 20110127 |
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Effective date of registration: 20110127 Address after: 116600 Anshan urban construction complex, Zhonghua Road, Dalian Development Zone, Liaoning, Dalian Patentee after: Dalian economic and Technological Development Zone Laifu Health Biotechnology Co.,Ltd. Address before: 115000, room 352, 25-3-2, Guangchang Road, Shenhe District, Liaoning, Shenyang, China Patentee before: Wang Jianying Effective date of registration: 20110127 Address after: 117004 commercial city, Benxi Development Zone, Liaoning Patentee after: Liaoning Cheng Da health food Co.,Ltd. Address before: 116600 Anshan urban construction complex, Zhonghua Road, Dalian Development Zone, Liaoning, Dalian Patentee before: Dalian economic and Technological Development Zone Laifu Health Biotechnology Co.,Ltd. |
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Address after: 117004, 1 East Road, Benxi economic and Technological Development Zone, Liaoning, Benxi Patentee after: LIAONING PROSPECTIVE BIOTECH Co.,Ltd. Address before: 117004 commercial city, Benxi Development Zone, Liaoning Patentee before: Liaoning Cheng Da health food Co.,Ltd. |
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