CN1506110A - Application of Bulgaria inquinans or its mycelium in preparing medicine - Google Patents
Application of Bulgaria inquinans or its mycelium in preparing medicine Download PDFInfo
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- CN1506110A CN1506110A CNA031285600A CN03128560A CN1506110A CN 1506110 A CN1506110 A CN 1506110A CN A031285600 A CNA031285600 A CN A031285600A CN 03128560 A CN03128560 A CN 03128560A CN 1506110 A CN1506110 A CN 1506110A
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Abstract
The present invention discloses the application of Bulgaria inquinans or its mycelium in preparing medicine. Bulgaria inquinans or its mycelium as medicinal material is soaked in ethanol to extract its effective components, with ethanol being recovered. The effective components is used in preparing medicine for psoriasis, dermatitis, leucoderma, eczema and other dermatosis. The medicine contains the ethanol extractive of Bulgaria inquinans or its mycelium and pharmaceutically acceptable supplementary material, and may be prepared into capsule, tablet, pill, soluble granule or oral liquid with excellent treating effect. Bulgaria inquinans or its mycelium may be cultured artificially.
Description
The application is that application number is: 00122191.4 the application divide an application.The applying date of original application is: on August 4th, 2000, name is called " Bulgaria inquinans or its mycelium artificial culture method and application ".
Technical field
The present invention relates to a kind of Bulgaria inquinans or its mycelium in the dermopathic medicinal application of preparation treatment.
Background technology
Nature has wild Bulgaria inquinans, but there is no both at home and abroad relevant for the report of using it for clinical treatment, does not also see the report of useful manual method cultivation cultivation Bulgaria inquinans, also it is not carried out remarkable work point from domestication work.Have very high clinical treatment to be worth owing to find Bulgaria inquinans, and at present natural wild and Bulgaria inquinans seldom, has significant application value so solve its artificial separation domesticating and cultivating technical problem.
Summary of the invention
Task of the present invention provides a kind of Bulgaria inquinans or the application of its mycelium in preparation treatment psoriasis, dermatitis, vitiligo or eczema dermatosis treating medicine, Bulgaria inquinans or its mycelium artificial culturing method and application, its available well Bulgaria inquinans is as the dermopathic specific medicament of treatment, be used for the treatment of dermatosiss such as psoriasis, vitiligo, dermatitis or eczema, and solved the problem that Bulgaria inquinans or its mycelium manually separate domesticating and cultivating, can artificially improve weight.
One of the object of the invention is achieved in that
A, carry out strain separating, from the whole sporophore of Bulgaria inquinans section fungus Bulgaria inquinans, isolate the Bulgaria inquinans mycelium, as the female original seed of planting of Bulgaria inquinans;
B, carry out female kind of first class inoculum and cultivate, adopt enriched medium to carry out mother and plant and separate the back switching, strain is sprouted the tailing edge media surface and is grown, form obvious bacterium colony, bacterium colony is grown more than 10 days at least, and as the Bulgaria inquinans strain, the parts by weight proportioning of above-mentioned enriched medium is:
Potato class 100-300 glucose or sucrose 10-30
Vitamin B1 5-15 agar 10-30 water 1000
Method is that the potato class is cut into little or thin slice, is put in that to add water boil in the pot thoroughly well cooked but not mushy to French fries, filters, and vitaminize B1 goes into filtrate in the pot in filtrate, and sugaring and agar with the slow fire heating, stir, and agar is dissolved, and add hot water and supply moisture.Be sub-packed in the incubator while hot;
Culture medium is made in c, Bulgaria inquinans secondary and three grades of cultivations earlier, the inoculation of sterilization back, and cultivation temperature is 15-35 ℃, waits to grow fine, and gets final product.
Above secondary and three grades of cultural methods are same, and three grades of cultivation results are to grow sporophore.
Above-mentioned culture medium can be used maize culture medium, and its parts by weight proportioning is: cook corn 60-95, Testa oryzae 10-30.Stone sound 0.5-1.5, material-water ratio are 1: 1.5-2.0, sterilization.
The also available sawdust medium of culture medium, its parts by weight proportioning is hardwood crumbs 60-90, Testa oryzae or Testa Tritici 10-30, sucrose 0.5-1.5, calcium carbonate or Gypsum Fibrosum powder 0.1-1.5, material-water ratio are 1: 1.5-2.0 takes by weighing raw material by proportioning, sugar is added in the entry, and other raw material blendings add sucrose solution.
The also available corn cob culture medium of culture medium, its parts by weight proportioning is corn cob 60-95, the sugared 10-30 of rice, Gypsum Fibrosum 0.5-1.5, material-water ratio are 1: 1.5-2.0, sterilization.
Two of the object of the invention is: Bulgaria inquinans or its mycelium medical material are extracted its effective ingredient with conventional Chinese medicine soak with ethanol method, reclaim ethanol, this effective ingredient is used for making treatment psoriasis, dermatitis, vitiligo or the dermopathic oral drugs of eczema.
One of the present invention's advantage is, the application in preparation treatment psoriasis, dermatitis, vitiligo or eczema dermatosis treating medicine of Bulgaria inquinans or its mycelium, and treatment psoriasis, dermatitis, vitiligo or the dermopathic medicine of eczema contain acceptable auxiliary on this Bulgaria inquinans or its mycelial ethanol extraction and the pharmaceutics, have good therapeutic effect.Bulgaria inquinans or its mycelial artificial separation domestication culture technique are reasonable, can reach predetermined purpose, and growth is fast, the output height, be fit to a large amount of cultivations, adopt manual method to solve the difficult problem that Bulgaria inquinans or its mycelium manually separate domestication first, have higher actual application value.
The specific embodiment:
Elaborate by the following examples, but not as a limitation of the invention.
Embodiment 1:
One, Bulgaria inquinans source and biological property.
(1) source: Bulgaria inquinans is the whole sporophore of Bulgaria inquinans section fungus Bulgaria inquinans.
(2) place of production: Jilin Province's Changbaishan area.
(3) morphological characteristic: immaturity apothecium intermediate recess, to go up slightly, end is thin, is the drabon color in the conical ascus back side, and the top color is shallow, and bottom color depth, card are pitchy.The sporophore list is given birth to or is grown thickly, grow thickly many persons 6-9, also has 3-4, ripe apothecium is open and flat, and the apothecium back side becomes pitchy, and color is darker, it is basidiospore that the black powder is arranged, many Dan Sheng, and it is less to grow thickly, the ascus card out-of-flatness that has, the edge undulate launches out a large amount of black ascus robes, powdery after the parachute-opening, it is short conical that the black handle is, and apothecium is as the open and flat cap shape of gill fungi.The high 1.93cm of immaturity sporophore, standby face width 1,34cm, ripe parachute-opening sporophore, high 2.13cm, the wide 3.95cm of card, the long 1.12cm of handle.
Two, strain separating
Adopt bark (length has mycelium), Ru Mu, meat bacteria organization and spore launch four kinds of modes,, wherein adopt meat bacteria organization's method and adopt strict sterile working from the Bulgaria inquinans mycelium in the sporophore intermediate section, cover with the female original seed of planting of Bulgaria inquinans of pipe in 20 days.
Three, the female kind cultivated (first class inoculum)
The employing enriched medium carries out mother and plants the switching of separation back, and strain sprouting tailing edge media surface has the place growth of the globule, and this bacterium sprouting is fast, and it is the obvious bacterium colony of basic point that strain connects 3-5 days interior the formation with globule point in back.Bacterium colony is not of uniform size, and the place is the most obvious with the inoculation point, and bacterium colony is bigger, bacterium colony point lamellar takes place, there is white lime color mycelium on the surface, sees that from the bacterium colony back side bacterium colony bottom becomes brown plaque, and the speckle size is close with the bacterium colony size, and old bacterium colony color depth, the petite color is shallow, and old bacterium colony has the big speckle associating of minority in the generation later stage, and petite independently becomes point-like, left and right sides bacterium colony covered with pipe in about 20 days, but was not in culture medium
Flat surfaced covers whole culture medium, still has the not long bacterium colony of more blank space, and colony growth progenesis speed is fast, and late growing stage speed is slack-off, may reduce relevant gradually with media surface moisture content.Separate female the kind and differentiate that mutually be defined as the Bulgaria inquinans strain, strain is insensitive to pH value by eating wood and sporophore,, now can carry out preservation and breed by the domestication that mother is planted.
Four, the Bulgaria inquinans second class inoculum is cultivated
Design 3 kinds of prescriptions: prescription 1 is a maize culture medium, and prescription 2 is the weed tree sawdust culture medium, and prescription 3 is corn cob culture medium (seeing appendix), and pH value is designed to 5,6,7 three numerical value normal-pressure sterilization cooling back inoculations, and cultivation temperature is 22-25 ℃ or 15 ℃-35 ℃.Can find out prescription 1 fast growth.Mycelia is strong, the general growth of 500ml Cans about 45 days, prescription 2 prescriptions 3 mycelia a little less than, but fast growth can cover with full bottle in general 35-40 days, found that the Bulgaria inquinans mycelia is insensitive to pH value, 5,6,7 three numerical value do not have obvious grown cultures.
Above-mentioned 4 kinds of formulation Example are as follows, and its compound method can be by aforesaid operations.
The enriched medium component
Annotate: Rhizoma Solani tuber osi can be used replacements such as Radix Ipomoeae, sweet potato, Rhizoma Dioscoreae.
The corn culture medium prescription
The sawdust medium prescription
| The g kind | Embodiment | |||
| ????1 | ????2 | ????3 | ????4 | |
| Hard assorted water bits | ????60 | ????70 | ????80 | ????90 |
| Testa oryzae | ????10 | ????30 | ||
| Or Testa Tritici | ????18 | ????22 | ||
| Sucrose | ????0.5 | ????1 | ????1.2 | ????1.5 |
| Calcium carbonate | ????0.1 | ????1.5 | ||
| Gypsum Fibrosum powder | ????0.5 | ????1 | ||
| Water | ????113 | ????161 | ????200 | ????185 |
The corn cob culture medium prescription
| The g kind | Embodiment | |||
| ????1 | ????2 | ????3 | ????4 | |
| Corn cob | ????60 | ????70 | ????80 | ????95 |
| Testa oryzae | ????10 | ????15 | ????20 | ????30 |
| Gypsum Fibrosum | ????0.5 | ????0.8 | ????1.2 | ????1.5 |
| Water | ????106 | ????154 | ????162 | ????202 |
Five, three grades of cultivations, the i.e. cultivation of Bulgaria inquinans.
Screen 3 prescriptions carry out respectively bottle plant and the bag plant.The same secondary of method, growth promoter is good.
Embodiment 2, extraction process:
Get Bulgaria inquinans or its fungal mycelium and pulverize (3-5 order), successively with 3 (2h of 5 times of amount alcohol reflux, 1h 1h) filters merging filtrate, ethanol is added in decompression, 1 part of taking liquid, 1.5 parts of mixing of adjuvant stir evenly, dry (about 60 ℃) pulverized 80 mesh sieves, and were encapsulated or make tablet, pill, electuary, oral liquid etc.The little hardship of this product, lung liver heart channel is returned in little acid, but heat-clearing and toxic substances removing, invigorating blood circulation dispels changes, and is used for the treatment of dermatosiss such as psoriasis, dermatitis, vitiligo, eczema.
Below be that the present invention is used for the treatment of dermopathic zoology test data:
Certain medical university's conclusion (of pressure testing): capsule is inhibited to epidermis cell model of hyperplasia (mice estrogen phase vaginal epithelial cell mitosis); (formation of mouse tail epidermis granular cell) has the promotion cutinization to epidermis cell parakeratosis model; And mix inhibited to a synthetic lymphocyte 3H-TdR of sending and receiving display list chrotoplast DNA; Explanation can alleviate maybe can reverse the psoriasis pathology change.
This capsule can obviously improve the thin amine itch-threshold of Cavia porcellus phosphoric acid; To rat chondrus ocellatus Holmes sufficient waste time swelling acute inflammation model and granuloma induced by implantation of cotton pellets chronic inflammation model, all has obvious antagonism; To the Mice Auricle blood capillary arteriole, thin vein bore are significantly increased, blood capillary is opened the quantity showed increased, and can obviously alleviate hemorheological abnormal change the blood stasis model rat; Show and have obviously antipruritic, antiinflammatory and invigorating blood circulation changed effect.Immunological testing shows that the skin delayed hypersensitivity to system's phagocytic function in the net of reflection nonspecific immunity function and reflection T cell-specific immunologic function does not have obvious influence, but the serum immunoglobulin IgG and the IgA of reflection humoral immunization are obviously increased, and IgM does not have obvious influence.
1, to the influence of psoriasis animal model
Purpose: psoriatic basic pathology characteristics are skin lesion position epidermis cell hyper-proliferative and parakeratosis.Certain medicine if can suppress the too fast hypertrophy of epidermis cell or induce the epidermis granular cell to generate, and promotes Keratoderma, then may have the psoriatic pharmacological action of treatment.It is active to be on the vagina of estrogen phase mice anti-cell proliferation, and the epithelial cell conversion is accelerated.The keratinization of mouse tail epidermis forms similar to people parapsoriasis person, lacks granular cell.Therefore, Chinese scholars as the psoriasis animal model, is estimated the effect of treatment psoriasis medicine with it.In addition, the synthetic synthetic and cell proliferation of skin epithelial cells DNA that reflects to a certain extent of lymphocyte DNA shows the influence to epidermis and hypodermal cell propagation indirectly.For this reason, choose above-mentioned three kinds of these capsules of experimental observation to psoriatic drug effect.
(1) to the mitotic influence of mice estrogen phase vaginal epithelial cell
Method: 60 of Kunming mouses, 20-30g.Lumbar injection diethylstilbestrol 0.2mg/ only every day 1 time, continuous 3 days, makes it be in the estrogen phase.Be divided into 5 groups at random on 4th, 12 every group.Every day, gastric infusion was 1 time, irritated stomach volume 0.25ml/10g, continuous 3 days.2 hours lumbar injection colchicine are put to death mice and are got vagina behind the last medicine after 2mg/k84 hour, 10% formalin fixed, the conventional film-making of paraffin embedding, HE dyeing.Light microscopic is the mitosis number of 300 vagina epithelium basal cells of counting down, and its percentage rate is a mitotic index.
Result: capsule 0.28 of the present invention, 0.56g/kg (7,14 times of quite clinical consumption per days, down together).Mice estrogen vaginal epithelial cell mitosis had obvious suppression effect (P＜0.05,0.01).
(2) influence that mouse tail epidermis granular cell is generated
Method: 60 of Kunming mouses, 20-30g.Be divided into 6 groups at random by table 2,10 every group, male and female half and half.Every day, gastric infusion was 1 time, and irritating the stomach volume is 0.25ml/10g, successive administration 20 days.Put to death whole mices in 2 hours after the last administration, get apart from Mus root of the tail portion 1.8cm place dorsal part skin one rectangularly, use 10% formalin fixed, the conventional film-making of paraffin embedding, HE dyeing.(400x) observes and counts eyepiece micrometer scope (132 μ m) endoparticle cell number in the scale (10) under light microscopic.
The result: this capsule 0.28,0.56g/kg have obvious facilitation (P＜0.05,0.01) to the generation of mouse tail granular cell.Increase 0.56g/kg add illumination group granular cell, and the Keratoderma layer also obviously thickens.
(3) mice T lymph is carefully run the synthetic influence of DNA
Method: 70 of Kunming mouses, 20-22g, male and female half and half.Be divided into 7 groups at random by sex, body weight.Blank group (the co-content distilled water is irritated stomach), (the co-content distilled water is irritated stomach to positive drug control group (cycli phosphate amine 15mg/kg lumbar injection) illumination matched group *, and accepts sun exposure 20 minutes after 2 hours, 8 joules/cm of illumination dose
2), this capsule is little, heavy dose adds illumination group * (capsule dosage and illumination condition are the same), 10 every group.Every day, gastric infusion was 1 time, and irritating the stomach volume is 0.25ml/10g), successive administration 7 days.Last administration and light are put to death, and get spleen.Mix method mensuration by ConA pouring commentaries on classics test 3H-TdR and mix radioactive intensity (cpm), reflect the DNA synthesis rate with this.
When this capsule was used for clinical treatment, patient's medication beyond the region of objective existence of taking off mourning clothes was also accepted sun exposure 20 minutes in back 12 hours in taking medicine.Add the illumination group so in this test, set up illumination group and capsule.
This capsule 0.56g/kg and 0.28,0.56g/kg add illumination person and all can obviously suppress lymphocyte DNA synthetic (P＜0.01 or 0.001) due to the ConA as a result.Adding its inhibitory action of illumination group strengthens to some extent.
Conclusion: this capsule has the inhibition proliferative effect to epidermis cell model of hyperplasia (mice estrogen phase vaginal epithelial cell mitosis); To epidermis cell parakeratosis model (generation of mouse tail epidermis granular cell), has the promotion cutinization; And the synthetic lymphocyte 3H-TdR of indirect demonstration epidermal dna being mixed inhibited, illumination can strengthen its inhibitory action behind the medicine.To sum up, this capsule can alleviate and maybe can reverse psoriasis pathology and change.
Claims (10)
1, Bulgaria inquinans or its mycelial medicinal application, wherein, with Bulgaria inquinans or its mycelium medical material soak with ethanol, extract its effective ingredient, reclaim ethanol, this effective ingredient is used for making treatment psoriasis, dermatitis, vitiligo or the dermopathic oral drugs of eczema.
2, the described application of claim 1, wherein, Bulgaria inquinans or its mycelial ethanol extraction and 1.5 parts of adjuvants are mixed and become described medicine, and Bulgaria inquinans or its mycelial ethanol extraction obtain by the following method: granularity is that 3-5 purpose Bulgaria inquinans or its fungal mycelium are measured alcohol reflux 3 times with 5 times successively, return time was respectively 2 hours, 1 hour, 1 hour, the merging filtrate decompression recycling ethanol is collected extract.
3, claim 1 or 2 described application, wherein, described treatment psoriasis, dermatitis, vitiligo or the dermopathic medicine of eczema contain acceptable auxiliary on this Bulgaria inquinans or its mycelial ethanol extraction and the pharmaceutics.
4, the described application of claim 3, wherein, described treatment psoriasis, dermatitis, vitiligo or the dermopathic medicine of eczema are peroral dosage form.
5, the described application of claim 4, wherein, described treatment psoriasis, dermatitis, vitiligo or the dermopathic medicine of eczema are capsule or tablet.
6, the described application of claim 4, wherein, described treatment psoriasis, dermatitis, vitiligo or the dermopathic medicine of eczema are pill, electuary or oral liquid.
7, claim 1 or 2 described application, wherein, Bulgaria inquinans or its mycelium medical material obtain by the artificial culture, and this breeding method comprises:
A, strain separating are isolated the Bulgaria inquinans mycelium from the whole sporophore of Bulgaria inquinans section fungus Bulgaria inquinans, as the female original seed of planting of Bulgaria inquinans;
B, the female kind of first class inoculum are cultivated, and adopt enriched medium to carry out mother's kind and separate the back switching, and strain is sprouted the tailing edge media surface and grown, form obvious bacterium colony, bacterium colony is grown more than 10 days at least, and as the Bulgaria inquinans strain, the parts by weight proportioning of above-mentioned enriched medium is:
Potato class 100-300 glucose or sucrose 10-30
Vitamin B1 5-15 agar 10-30 water 1000
C, Bulgaria inquinans secondary and three grades of cultivations, cultivation temperature is 15-35 ℃.
8, the described application of claim 7, wherein, the culture medium of secondary and three grades of incubation is a maize culture medium, its parts by weight proportioning is: cook corn 60-95, Testa oryzae 10-30, Gypsum Fibrosum 0.5-1.5, material-water ratio are 1: 1.5-2.0.
9, the described application of claim 7, wherein, the culture medium of secondary and three grades of incubation is a sawdust medium, its parts by weight proportioning is: hardwood crumbs 60-90, Testa oryzae or Testa Tritici 10-30, sucrose 0.5-1.5, calcium carbonate or Gypsum Fibrosum powder 0.1-1.5, material-water ratio are 1: 1.5-2.0.
10, the described application of claim 7, wherein the culture medium of secondary and three grades of incubation is the corn cob culture medium, its parts by weight proportioning is: corn cob 60-95, the sugared 10-30 of rice, Gypsum Fibrosum 0.5-1.5, material-water ratio are 1: 1.5-2.0.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031285600A CN1506110A (en) | 2000-08-04 | 2000-08-04 | Application of Bulgaria inquinans or its mycelium in preparing medicine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031285600A CN1506110A (en) | 2000-08-04 | 2000-08-04 | Application of Bulgaria inquinans or its mycelium in preparing medicine |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00122191 Division CN1279005A (en) | 2000-08-04 | 2000-08-04 | Artificial culture method and application of Jiaotuoluo or its mycelium |
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| Publication Number | Publication Date |
|---|---|
| CN1506110A true CN1506110A (en) | 2004-06-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA031285600A Pending CN1506110A (en) | 2000-08-04 | 2000-08-04 | Application of Bulgaria inquinans or its mycelium in preparing medicine |
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| CN (1) | CN1506110A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101757104B (en) * | 2009-12-22 | 2012-04-11 | 北大方正集团有限公司 | Medicament for treating psoriasis |
| CN103505535A (en) * | 2012-06-29 | 2014-01-15 | 北大方正集团有限公司 | Medicine used for treating psoriasis as well as extraction method, extract and application thereof |
-
2000
- 2000-08-04 CN CNA031285600A patent/CN1506110A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101757104B (en) * | 2009-12-22 | 2012-04-11 | 北大方正集团有限公司 | Medicament for treating psoriasis |
| CN103505535A (en) * | 2012-06-29 | 2014-01-15 | 北大方正集团有限公司 | Medicine used for treating psoriasis as well as extraction method, extract and application thereof |
| CN103505535B (en) * | 2012-06-29 | 2015-05-06 | 北大方正集团有限公司 | Medicine used for treating psoriasis as well as extraction method, extract and application thereof |
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