CN1502626A - Novel human protein with cancer-inhibiting function and coding sequence thereof - Google Patents
Novel human protein with cancer-inhibiting function and coding sequence thereof Download PDFInfo
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Abstract
The present invention discloses a novel human protein with the function of inhibiting cancer, polynucleotide for coding said polypeptide and method for producing said polypeptide by means of recombination technology. Said invention also discloses the method for curing several diseases, such as cancer, etc. by using said polypeptide. Said invention also discloses the agonist resisting said polypeptide and its therapeutic action, and also discloses the application of the polynucleotide for coding said human protein with anti-cancer function.
Description
Technical field
The invention belongs to biological technical field, specifically, the present invention relates to new coding and have the proteic polynucleotide of people of cancer suppressing function and the polypeptide of this polynucleotide encoding.The invention still further relates to the purposes and the preparation of these polynucleotide and polypeptide.
Background technology
The research of people's gene group is international focus at present, removes human chromosome DNA large scale sequencing, outside the method for expressed sequence order-checking (EST), also lacks the screening that begins from function and has the high-throughout method of functional gene.
Cancer is one of principal disease of harm humans health.In order to treat effectively and prophylaxis of tumours, people more and more pay close attention to genetic treatment of tumor at present.Therefore, this area presses for people's albumen and the agonist/inhibitor thereof that development research has cancer suppressing function.
Summary of the invention
The purpose of this invention is to provide the new people's protein polypeptide of a class with cancer suppressing function with and fragment, analogue and derivative.
Another object of the present invention provides the polynucleotide of these polypeptide of coding.
Another object of the present invention provides the method for these polypeptide of production and the purposes of this polypeptide and encoding sequence.
In a first aspect of the present invention, novel isolated protein polypeptide with cancer suppressing function is provided, it comprises the polypeptide of the aminoacid sequence with the group of being selected from down: SEQ ID NO:3,6,9,12,15,18,21,24,27,30; Or its conservative property variation polypeptide or its active fragments or its reactive derivative.Preferably, this polypeptide is the polypeptide with aminoacid sequence of the group of being selected from down: SEQ ID NO:3,6,9,12,15,18,21,24,27,30.
In a second aspect of the present invention, a kind of isolating polynucleotide are provided, it comprises a nucleotide sequence, and this nucleotide sequence is shown at least 85% homogeny with a kind of nucleotides sequence that is selected from down group: the polynucleotide of the above-mentioned protein polypeptide with cancer suppressing function of (a) encoding; (b) with polynucleotide (a) complementary polynucleotide.Preferably, the polypeptide of this polynucleotide encoding has the aminoacid sequence of the group of being selected from down: SEQ ID NO:3,6,9,12,15,18,21,24,27,30.More preferably, the sequence of these polynucleotide is selected from down group: SEQ ID NO:2,5,8,11,14,17,20,23,26,29 coding region sequence or full length sequence.
In a third aspect of the present invention, the carrier that contains above-mentioned polynucleotide is provided, and has been transformed or host cell of transduceing or the host cell that is directly transformed or transduce by above-mentioned polynucleotide by this carrier.
In a fourth aspect of the present invention, the preparation method who prepares the polypeptide of the protein-active with cancer suppressing function is provided, this method comprises: (a) have under the proteic condition of cancer suppressing function suitable the expression, cultivate the above-mentioned host cell that is transformed or transduce: the polypeptide of (b) isolating the protein-active with cancer suppressing function from culture.
In a fifth aspect of the present invention, provide and above-mentioned protein polypeptide specificity bonded antibody with cancer suppressing function.The nucleic acid molecule that can be used for detecting also is provided, and it contains, and continuous 10 Nucleotide are to full length nucleotide in the above-mentioned polynucleotide, and preferably it contains the about 15-1000 of a successive Nucleotide.
In a sixth aspect of the present invention, a kind of pharmaceutical composition is provided, it contains the protein polypeptide and the pharmaceutically acceptable carrier with cancer suppressing function of the present invention of safe and effective amount.These pharmaceutical compositions can be treated illnesss such as cancer and cellular abnormality propagation.
Others of the present invention are because the disclosure of this paper is conspicuous to those skilled in the art.
Embodiment
The present invention adopts large-scale cDNA clone transfection cancer cells, has on the basis of cancer suppressing action in acquisition, proves new gene through order-checking, further obtains full length cDNA clone.DNA transfection evidence, the albumen with cancer suppressing function of the present invention has the effect that suppresses clone's formation, its inhibiting rate 〉=50% to liver cancer cell 7721.
As used herein, " isolating " is meant that material separates (if natural substance, primal environment promptly is a natural surroundings) from its primal environment.Do not have separation and purification as polynucleotide under the native state in the active somatic cell and polypeptide, but same polynucleotide or polypeptide as from native state with in other materials that exist separately, then for separation and purification.
As used herein, " isolating albumen or polypeptide with cancer suppressing function " is meant that the protein polypeptide with cancer suppressing function is substantially free of natural relative other albumen, lipid, carbohydrate or other material.Those skilled in the art can have the albumen of cancer suppressing function with the purified technology of protein purifying of standard.Basically pure polypeptide can produce single master tape on non-reduced polyacrylamide gel.
Polypeptide of the present invention can be recombinant polypeptide, natural polypeptides, synthetic polypeptide, preferred recombinant polypeptide.Polypeptide of the present invention can be the product of natural purifying, or the product of chemosynthesis, or uses recombinant technology to produce from protokaryon or eucaryon host (for example, bacterium, yeast, higher plant, insect and mammalian cell).The host used according to the recombinant production scheme, polypeptide of the present invention can be glycosylated, maybe can be nonglycosylated.Polypeptide of the present invention also can comprise or not comprise initial methionine residues.
The present invention also comprises the proteic fragment of the people with cancer suppressing function, derivative and analogue.As used herein, term " fragment ", " derivative " are meant with " analogue " and keep natural identical biological function or the active polypeptide of people's albumen with cancer suppressing function of the present invention basically.Polypeptide fragment of the present invention, derivative or analogue can be that (i) has one or more conservative or substituted polypeptide of non-conservation amino-acid residue (preferred conservative amino acid residue), and the amino-acid residue of such replacement can be also can not encoded by genetic code, or (ii) in one or more amino-acid residues, has a polypeptide of substituted radical, or (iii) mature polypeptide and another compound (such as the compound that prolongs the polypeptide transformation period, polyoxyethylene glycol for example) merge formed polypeptide, or (iv) additional aminoacid sequence is fused to this peptide sequence and the polypeptide that forms (as leader sequence or secretion sequence or be used for the sequence or the proteinogen sequence of this polypeptide of purifying).According to the instruction of this paper, these fragments, derivative and analogue belong to the known scope of those skilled in the art.
Polynucleotide of the present invention can be dna form or rna form.Dna form comprises the DNA of cDNA, genomic dna or synthetic.DNA can be strand or double-stranded.DNA can be coding strand or noncoding strand.Be example with LP4790 albumen (in this application, its clone numbering is adopted in proteinic name), the coding region sequence of encoding mature polypeptide can be identical with the coding region sequence shown in the SEQ ID NO:2 or the varient of degeneracy.As used herein, " varient of degeneracy " is meant that for LP4790 coding has the protein of SEQ ID NO:3, but with the differentiated nucleotide sequence of coding region sequence shown in the SEQ ID NO:2.Be example with LP4791 albumen again, the coding region sequence of encoding mature polypeptide can be identical with the coding region sequence shown in the SEQ ID NO:5 or the varient of degeneracy; " varient of degeneracy " is meant that for LP4791 coding has the protein of SEQ ID NO:6, but with the differentiated nucleotide sequence of coding region sequence shown in the SEQ ID NO:5.Have the albumen of cancer suppressing function for of the present invention other, can the rest may be inferred.
The polynucleotide of encoding mature polypeptide comprise: the encoding sequence of an encoding mature polypeptide; The encoding sequence of mature polypeptide and various additional code sequence; Encoding sequence of mature polypeptide (with optional additional code sequence) and non-coding sequence.
Term " polynucleotide of coded polypeptide " can be the polynucleotide that comprise this polypeptide of encoding, and also can be the polynucleotide that also comprise additional code and/or non-coding sequence.
The invention still further relates to the varient of above-mentioned polynucleotide, its coding has the polypeptide of identical aminoacid sequence or fragment, analogue and the derivative of polypeptide with the present invention.The varient of these polynucleotide can be the allelic variant of natural generation or the varient that non-natural takes place.These nucleotide diversity bodies comprise and replace varient, deletion mutation body and insert varient.As known in the art, allelic variant is the replacement form of polynucleotide, and it may be replacement, disappearance or the insertion of one or more Nucleotide, but can be from not changing the function of its encoded polypeptides in fact.
The invention still further relates to and above-mentioned sequence hybridization and two sequences between have at least 50%, preferably at least 70%, the polynucleotide of at least 80% homogeny more preferably.The present invention be more particularly directed under stringent condition and the interfertile polynucleotide of polynucleotide of the present invention.In the present invention, " stringent condition " is meant: (1) than hybridization under low ionic strength and the comparatively high temps and wash-out, as 0.2 * SSC, and 0.1%SDS, 60 ℃; Or (2) hybridization the time is added with denaturing agent, as 50% (v/v) methane amide, 0.1% calf serum/0.1%Ficoll, 42 ℃ etc.; Or (3) only at the homogeny between the two sequences at least more than 95%, be more preferably 97% and just hybridize when above.And, the polypeptide of interfertile polynucleotide encoding and SEQ IDNO; Mature polypeptide shown in 3 has identical biological function (is example with LP4790 albumen) and activity.
The invention still further relates to nucleic acid fragment with above-mentioned sequence hybridization.As used herein, the length of " nucleic acid fragment " contains 15 Nucleotide at least, better is at least 30 Nucleotide, is more preferably at least 50 Nucleotide, preferably more than at least 100 Nucleotide.The amplification technique (as PCR) that nucleic acid fragment can be used for nucleic acid has the proteic polynucleotide of cancer suppressing function to determine and/or to separate to encode.
Polypeptide among the present invention and polynucleotide preferably provide with isolating form, more preferably are purified to homogeneous.
Dna sequence dna of the present invention can obtain with several method.For example, with hybridization technique DNA isolation well known in the art.These technology including, but not limited to; 1) with probe and genome or the hybridization of cDNA library to detect homology nucleotide sequence and 2) antibody screening of expression library to be to detect the dna fragmentation of the clone with common structure feature.
The proteic specific DNA fragment sequence that coding has cancer suppressing function produces also and can obtain with following method: 1) separate double chain DNA sequence from genomic dna; 2) the chemical synthesising DNA sequence is to obtain the double-stranded DNA of required polypeptide.
When the whole aminoacid sequence of the polypeptide product of needs was known, the direct chemical of dna sequence dna is synthetic to be the method for often selecting for use.When if required amino acid whose whole sequence is not known, the direct chemical of dna sequence dna is synthetic to be impossible, and the method for selecting for use is the separation of cDNA sequence.The standard method that separates interested cDNA is from the donorcells separating mRNA of this gene of high expression level and carries out reverse transcription, forms plasmid or phage cDNA library.Extract the existing multiple proven technique of method of mRNA, test kit also can obtain (Qiagene) from commercial channels.And the construction cDNA library also is usual method (Sambrook, et al., Molecular Cloning, A Laboratory Manual, Cold SpringHarbor Laboratory.New York, 1989).Also can obtain the cDNA library of commercial offers, as the different cDNA library of Clontech company.When being used in combination the polymeric enzyme reaction technology, even few expression product also can be cloned.
Available ordinary method is screened gene of the present invention from these cDNA libraries.These methods include, but is not limited to: (1) DNA-DNA or DNA-RNA hybridization; (2) function of marker gene occurs or forfeiture; (3) mensuration has the level of the proteic transcript of cancer suppressing function; (4), detect the protein product of genetic expression by immunological technique or mensuration biologic activity.Aforesaid method can singly be used, but also several different methods combined utilization.
In (1) kind method, hybridizing used probe is and any a part of homology of polynucleotide of the present invention that at least 15 Nucleotide of its length better are at least 30 Nucleotide, are more preferably at least 50 Nucleotide, preferably at least 100 Nucleotide.In addition, the length of probe within 2kb, preferably is within the 1kb usually.Probe used herein is the dna sequence dna of chemosynthesis on the basis of gene DNA sequence information of the present invention normally.Gene of the present invention itself or fragment are certainly as probe.The mark of dna probe can be used radio isotope, fluorescein or enzyme (as alkaline phosphatase) etc.
In (4) kind method, detect the protein product of protein gene expression and can use immunological technique such as Western blotting, radioimmunoprecipitation, enzyme-linked immunosorbent assay (ELISA) etc. with cancer suppressing function.
Use method (Saiki, the et al.Science 1985 of round pcr DNA amplification/RNA; 230:1350-1354) be optimized for acquisition gene of the present invention.When particularly being difficult to obtain the cDNA of total length from the library, can preferably use RACE method (the terminal rapid amplifying method of RACE-cDNA), the primer that is used for PCR can suitably be selected according to sequence information of the present invention disclosed herein, and available ordinary method is synthetic.Available ordinary method is as the DNA/RNA fragment by gel electrophoresis separation and purifying amplification.
The gene of the present invention that obtains as mentioned above, perhaps the available ordinary method of mensuration of the nucleotide sequence of various dna fragmentations etc. such as dideoxy chain termination (Sanger et al.PNAS, 1977,74:5463-5467).This class nucleotide sequencing is available commercial sequencing kit etc. also.In order to obtain the cDNA sequence of total length, order-checking need be carried out repeatedly.Sometimes need to measure a plurality of clones' cDNA sequence, just can be spliced into the cDNA sequence of total length.
The present invention also relates to comprise the carrier of polynucleotide of the present invention, and the host cell that produces through genetically engineered with carrier of the present invention or albumen coded sequence with cancer suppressing function, and the method that produces polypeptide of the present invention through recombinant technology.
By the recombinant DNA technology of routine, can utilize polymerized nucleoside acid sequence of the present invention to can be used to express or produce the protein polypeptide with cancer suppressing function of reorganization.In general following steps are arranged:
(1). have the proteic polynucleotide of people (or varient) of cancer suppressing function with coding of the present invention, or transform or the transduction proper host cell with the recombinant expression vector that contains these polynucleotide;
(2). the host cell of in suitable medium, cultivating;
(3). separation, protein purification from substratum or cell.
Among the present invention, the people's albumen polynucleotide sequence with cancer suppressing function can be inserted in the recombinant expression vector.Term " recombinant expression vector " refers to that bacterial plasmid well known in the art, phage, yeast plasmid, vegetable cell virus, mammalian cell virus are as adenovirus, retrovirus or other carriers.The carrier of Shi Yonging includes but not limited in the present invention: the expression vector based on T7 of expressing in bacterium; The pMSXND expression vector of in mammalian cell, expressing and at the carrier that derives from baculovirus of expressed in insect cells.In a word, as long as can duplicate in host and stablize, any plasmid and carrier can be used.A key character of expression vector is to contain replication orgin, promotor, marker gene and translation controlling elements usually.
Method well-known to those having ordinary skill in the art can be used to make up and contains people's encoding histone dna sequence dna with cancer suppressing function and suitable transcribing/the translate expression vector of control signal.These methods comprise extracorporeal recombinant DNA technology, DNA synthetic technology, the interior recombinant technology of body etc.Described dna sequence dna can effectively be connected on the suitable promotor in the expression vector, and is synthetic to instruct mRNA.The representative example of these promotors has: colibacillary lac or trp promotor; Lambda particles phage P
LPromotor; Eukaryotic promoter comprises LTRs and some other known may command gene expression promoter in protokaryon or eukaryotic cell or its virus of CMV immediate early promoter, early stage and late period SV40 promotor, retrovirus.Expression vector also comprises ribosome bind site and the transcription terminator that translation initiation is used.
In addition, expression vector preferably comprises one or more selected markers, to be provided for selecting the phenotypic character of transformed host cells, cultivate Tetrahydrofolate dehydrogenase, neomycin resistance and the green fluorescent protein (GFP) of usefulness as eukaryotic cell, or be used for colibacillary tsiklomitsin or amicillin resistance.
Comprise the carrier of above-mentioned suitable dna sequence dna and suitable promotor or control sequence, can be used to transform appropriate host cell, so that it can marking protein.
Host cell can be a prokaryotic cell prokaryocyte, as bacterial cell; Or eukaryotic cell such as low, as yeast cell; Or higher eucaryotic cells, as mammalian cell.Representative example has: intestinal bacteria, streptomyces; The bacterial cell of Salmonella typhimurium; Fungal cell such as yeast; Vegetable cell; The insect cell of fruit bat S2 or Sf9; The zooblast of CHO, COS or Bowes melanoma cells etc.
When polynucleotide of the present invention are expressed in higher eucaryotic cells, be enhanced if will make to transcribe when in carrier, inserting enhancer sequence.Enhanser is the cis acting factor of DNA, and nearly 10 to 300 base pairs act on promotor transcribing with enhancing gene usually.Can for example be included in the SV40 enhanser of 100 to 270 base pairs of replication origin side in late period one, at the polyoma enhanser of replication origin side in late period one and adenovirus enhanser etc.
Persons skilled in the art all know how to select appropriate carriers, promotor, enhanser and host cell.
Can carry out with routine techniques well known to those skilled in the art with the recombinant DNA transformed host cell.When the host was prokaryotic organism such as intestinal bacteria, the competent cell that can absorb DNA can be used CaCl in exponential growth after date results
2Method is handled, and used step is well-known in this area.Alternative is to use MgCl
2If desired, transforming also the method for available electroporation carries out.When the host is an eukaryote, can select following DNA transfection method for use: coprecipitation of calcium phosphate method, conventional mechanical method such as microinjection, electroporation, liposome packing etc.
The transformant that obtains can be cultivated with ordinary method, expresses the polypeptide of coded by said gene of the present invention.According to used host cell, used substratum can be selected from various conventional substratum in the cultivation.Under the condition that is suitable for the host cell growth, cultivate.After host cell grows into suitable cell density, induce the promotor of selection with suitable method (as temperature transition or chemical induction), cell is cultivated for some time again.
Recombinant polypeptide in the above methods can wrap by in cell, extracellular or on cytolemma, express or be secreted into the extracellular.If desired, can utilize its physics, the separating by various separation methods with other characteristic and the albumen of purification of Recombinant of chemistry.These methods are well-known to those skilled in the art.The example of these methods includes, but are not limited to: conventional renaturation handles, with protein precipitant handle (salt analysis method), centrifugal, the broken bacterium of infiltration, superly handle, the combination of super centrifugal, sieve chromatography (gel-filtration), adsorption chromatography, ion exchange chromatography, high performance liquid chromatography (HPLC) and other various liquid chromatography (LC) technology and these methods.
The people's albumen or the polypeptide with cancer suppressing function of reorganization are of use in many ways.These purposes include, but is not limited to: directly have the disease (as cancer) due to the low or forfeiture of the protein function of cancer suppressing function as pharmacological agent and be used to screen and promote or antagonism has antibody, polypeptide or other part of the protein function of cancer suppressing function.For example, antibody can be used for activating or suppressing to have the proteic function of people of cancer suppressing function.The people's protein screening peptide library that has a cancer suppressing function with the reorganization of expressing can be used for seeking the peptide molecule that can suppress or stimulate the people's protein function with cancer suppressing function of therapeutic value.
The present invention also provides screening of medicaments to improve (agonist) or check the method that (antagonist) has the proteic medicament of people of cancer suppressing function to identify.Agonist improves the biological function such as stimulate cellular proliferation of the people's albumen with cancer suppressing function, and antagonist prevention disorder such as the various cancer relevant with cell hyperproliferation with treatment.For example, can be in the presence of medicine, the proteic film preparation of people that mammalian cell or expression is had cancer suppressing function is cultivated with the people's albumen with cancer suppressing function of mark.Measure the medicine raising then or check this interactional ability.
The proteic antagonist of people with cancer suppressing function comprises antibody, compound, acceptor disappearance thing and the analogue etc. that filter out.The proteic antagonist of people with cancer suppressing function can and be eliminated its function with the people's protein binding with cancer suppressing function, or suppresses to have the proteic generation of people of cancer suppressing function, or combines with the avtive spot of polypeptide and to make polypeptide can not bring into play biological function.The proteic antagonist of people with cancer suppressing function can be used for therepic use.
In screening during as the compound of antagonist, albumen of the present invention can be added during bioanalysis measures, determine by measuring albumen and the interaction between its acceptor that compounds affect has cancer suppressing function whether compound is antagonist.With the same quadrat method of above-mentioned SCREENED COMPOUND, can filter out the acceptor disappearance thing and the analogue of antagonist action.
Polypeptide of the present invention can be directly used in disease treatment, for example, and various malignant tumours and cellular abnormality propagation etc.
Polypeptide of the present invention, and fragment, derivative, analogue or their cell can be used as antigen to produce antibody.These antibody can be polyclone or monoclonal antibody.Polyclonal antibody can obtain by the method with this polypeptide direct injection animal.The technology of preparation monoclonal antibody comprises hybridoma technology, three knurl technology, people B-quadroma technology, EBV-hybridoma technology etc.
Can be with polypeptide of the present invention and antagonist and suitable pharmaceutical carrier combination back use.These carriers can be water, glucose, ethanol, salt, damping fluid, glycerine and their combination.Composition comprises the polypeptide or the antagonist of safe and effective amount and carrier and the vehicle that does not influence effect of drugs.These compositions can be used as medicine and are used for disease treatment.
The present invention also provides medicine box or the test kit that contains one or more containers, and one or more medicinal compositions compositions of the present invention are housed in the container.With these containers, can have by the given indicative prompting of government authorities of making, using or selling medicine or biological products, the government authorities that this prompting reflects production, uses or sells permits it to use on human body.In addition, polypeptide of the present invention can be used in combination with other treatment compound.
Pharmaceutical composition can be with mode administration easily, as by in part, intravenously, intraperitoneal, intramuscular, subcutaneous, the nose or the route of administration of intracutaneous.Albumen with cancer suppressing function comes administration with the amount that treats and/or prevents concrete indication effectively.The proteic amount with cancer suppressing function and the dosage range that are applied to the patient will depend on many factors, as administering mode, person's to be treated healthiness condition and diagnostician's judgement.
The proteic polynucleotide of people with cancer suppressing function also can be used for multiple therapeutic purpose.Gene therapy technology can be used for treating since have that the proteic nothing of cancer suppressing function is expressed or the proteic expression with cancer suppressing function of unusual/non-activity due to cell proliferation, growth or metabolic disturbance.The gene therapy vector of reorganization can be used for treating the protein expression with cancer suppressing function or the disease of active caused by abnormal.Deriving from the expression vector of virus such as protein gene that retrovirus, adenovirus, adeno-associated virus (AAV), hsv, parvovirus etc. can be used for having cancer suppressing function is transferred in the cell.The method that structure carries the recombinant viral vector of the protein gene with cancer suppressing function be found in existing document (Sambrook, etal.).The people protein gene of reorganization with cancer suppressing function can be packaged in the liposome and be transferred in the cell in addition.
Suppress to have cancer suppressing function people's protein mRNA oligonucleotide (comprising sense-rna and DNA) and ribozyme also within the scope of the invention.Ribozyme is the enzyme sample RNA molecule that a kind of energy specificity is decomposed specific RNA, and its mechanism of action is to carry out the endonuclease effect after ribozyme molecule and the hybridization of complementary target RNA-specific.The RNA of antisense and DNA and ribozyme can obtain with existing any RNA or DNA synthetic technology, as the technology widespread use of solid phase phosphoamide chemical synthesis synthetic oligonucleotide.Antisense rna molecule can be transcribed acquisition by the dna sequence dna of this RNA that encodes in external or body.This dna sequence dna has been incorporated into the downstream of rna polymerase promoter of carrier.In order to increase the stability of nucleic acid molecule, available several different methods is modified it, and as increasing the sequence length of both sides, the connection between the ribonucleoside is used phosphoric acid thioester bond or peptide bond but not phosphodiester bond.
Polynucleotide imports tissue or intracellular method comprises: directly be injected into polynucleotide in the in-vivo tissue; Or external by carrier (as virus, phage or plasmid etc.) earlier with the polynucleotide transfered cell in, again cell is transplanted in the body etc.
Polypeptide of the present invention also can be used as the peptide spectrum analysis, for example, the polypeptide available physical, chemistry or enzyme carry out the specificity cutting, and carry out the two-dimentional or three-dimensional gel electrophoresis analysis of one dimension.
The present invention also provides the antibody at the people's proteantigen determinant with cancer suppressing function.These antibody include, but is not limited to: the fragment that polyclonal antibody, monoclonal antibody, chimeric antibody, single-chain antibody, Fab fragment and Fab expression library produce.These antibody can prepare with ordinary method.The anti-proteic antibody of people with cancer suppressing function can be used in the immunohistochemistry technology, detects the people's albumen with cancer suppressing function in the biopsy specimen.
With the also available labelled with radioisotope of the protein bound monoclonal antibody of the people with cancer suppressing function, inject in the body and can follow the tracks of its position and distribution.Antibody among the present invention can be used for treating or prevents and the relevant disease of people's albumen with cancer suppressing function.The antibody that gives suitable dosage can stimulate or block proteic generation of the people with cancer suppressing function or activity.
Antibody also can be used for designing the immunotoxin at a certain privileged sites in the body.As have cancer suppressing function people's albumen high-affinity monoclonal antibody can with bacterium or plant poison (as diphtheria toxin, ricin, abrine etc.) covalent attachment.
Available people's albumen or the polypeptide immune animal of the production of polyclonal antibody with cancer suppressing function, as rabbit, mouse, rat etc.Multiple adjuvant can be used for the enhancing immunity reaction, includes but not limited to freund's adjuvant etc.
Have cancer suppressing function people's protein monoclonal antibody can with hybridoma technology production (Kohler and Milstein.Nature, 1975,256:495-497).With the variable region bonded chimeric antibody in human constant region and inhuman source can with existing technology production (Morrison et al, PNAS, 1985,81:6851).And the technology of existing manufacture order chain antibody (U.S.PatNo.4946778) also can be used for producing the anti-proteic single-chain antibody of people with cancer suppressing function.
Can be incorporated into the rondom polypeptide storehouse that solid formation forms by the various amino acid that may make up by screening with the protein bound peptide molecule of the present invention obtains.During screening, must carry out mark to people's protein molecular with cancer suppressing function.
The invention still further relates to quantitatively and detection and localization has the diagnostic testing process of people's protein level of cancer suppressing function.These tests are known in the art, and comprise that FISH measures and radioimmunoassay.The people's protein level that is detected in the test with cancer suppressing function, the disease that can have the importance of people's albumen in various diseases of cancer suppressing function with laying down a definition and be used to diagnose albumen to work with cancer suppressing function.
Proteic polynucleotide with cancer suppressing function can be used for having the diagnosis and the treatment of the protein related diseases of cancer suppressing function.Aspect diagnosis, the proteic polynucleotide with cancer suppressing function can be used for detecting have cancer suppressing function proteic expression whether or under morbid state, have an abnormal exprssion of cancer suppressing function.As the protein D NA sequence with cancer suppressing function can be used for the hybridization of biopsy specimen is had with judgement the proteic abnormal expression of cancer suppressing function.Hybridization technique comprises the Southern blotting, Northern blotting, in situ hybridization etc.These technological methods all are disclosed mature technologies, and relevant test kit all can obtain from commercial channels.Part or all of polynucleotide of the present invention can be used as probe stationary on microarray (Microarray) or DNA chip (being called " gene chip " again), is used for analyzing the differential expression analysis and the gene diagnosis of tissue gene.Carry out RNA-polymerase chain reaction (RT-PCR) amplification in vitro with the special primer of the albumen with cancer suppressing function and also can detect proteic transcription product with cancer suppressing function.
The sudden change that detection has the protein gene of cancer suppressing function also can be used for diagnosing the relevant disease of albumen with cancer suppressing function.Form with protein mutation of cancer suppressing function comprises that to have point mutation that the protein D NA sequence of cancer suppressing function compares, transposition, disappearance, reorganization and other any unusual etc. with normal wild type.Available existing technology such as Southern blotting, dna sequence analysis, PCR and in situ hybridization detect sudden change.In addition, sudden change might influence proteic expression, therefore can judge indirectly that with Northern blotting, Western blotting gene has or not sudden change.
Sequence of the present invention identifies it also is valuable to karyomit(e).These sequences can be specifically at certain bar human chromosome particular location and and can with its hybridization.At present, need to identify the concrete site of each gene on the karyomit(e).Yet have only chromosomal marker thing seldom to can be used for the marker chromosomes position now based on actual sequence data (repetition polymorphism).For these sequences are associated with disease related gene.The first step is positioned dna sequence dna of the present invention on the karyomit(e) exactly.
In brief, prepare PCR primer (preferred 15-35bp), sequence can be positioned on the karyomit(e) according to cDNA.Then, these primers are used for the somatocyte hybrid cell that the PCR screening contains each bar human chromosome.Have only those hybrid cells that contain corresponding to the people's gene of primer can produce the fragment of amplification.
The PCR localization method of somatocyte hybrid cell is that DNA is navigated to concrete chromosomal quick method.Use Oligonucleolide primers of the present invention,, can utilize one group to realize inferior location from specific chromosomal fragment or a large amount of genomic clone by similar approach.Other the similar strategy that can be used for chromosomal localization comprises in situ hybridization, uses the karyomit(e) prescreen and the hybridization preliminary election of the airflow classification of mark, thereby makes up the special cDNA storehouse of karyomit(e).
The cDNA clone is carried out fluorescence in situ hybridization (FISH) with Metaphase Chromosome, can in a step, accurately carry out chromosomal localization.The summary of this technology is referring to Verma etc., Human Chromosomes:a Manual of BasicTechniques, Pergamon Press, New York (1988).
In case sequence is positioned to chromosome position accurately, the physical location of this sequence on karyomit(e) just can be associated with the gene map data.These data for example are found in, V.Mckusick, Mendelian Inheritance in Man (can by with the online acquisition of Johns Hopkins University Welch Medical Library).Can pass through linkage analysis then, determine gene and navigated to relation between the disease on the chromosomal region already.
Then, need to measure ill and not cDNA between diseased individuals or genome sequence difference.If observe certain sudden change in some or all of diseased individuals, and this sudden change is not observed in any normal individual, then this sudden change may be the cause of disease of disease.More ill and diseased individuals not is usually directed at first seek the variation of structure in the karyomit(e), as from the horizontal visible of karyomit(e) or use based on detectable disappearance of the PCR of cDNA sequence or transposition.
Pyrenoids thuja acid full length sequence or its fragment with cancer suppressing function of the present invention can obtain with the method for pcr amplification method, recombination method or synthetic usually.For the pcr amplification method, can be disclosed according to the present invention about nucleotide sequence, especially open reading frame sequence designs primer, and with commercially available cDNA storehouse or by the prepared cDNA storehouse of ordinary method well known by persons skilled in the art as template, amplification and must relevant sequence.When sequence is longer, usually needs to carry out twice or pcr amplification repeatedly, and then the fragment that each time amplifies is stitched together by proper order.
In case obtained relevant sequence, just can obtain relevant sequence in large quantity with recombination method.This normally is cloned into carrier with it, changes cell again over to, separates obtaining relevant sequence then from the host cell after the propagation by ordinary method.
In addition, also the method for available synthetic is synthesized relevant sequence, especially fragment length more in short-term.Usually, by first synthetic a plurality of small segments, and then connect and to obtain the very long fragment of sequence.
At present, can be fully come the dna sequence dna of code book invention albumen (or its fragment, or derivatives thereof) by chemosynthesis.This dna sequence dna can be introduced then in the various dna moleculars (as carrier) and cell in this area.In addition, also can will suddenly change and introduce in the protein sequence of the present invention by chemosynthesis.
In addition, because the albumen with cancer suppressing function of the present invention has the natural acid sequence that is derived from the people, therefore, compare with the albumen of the same clan that derives from other species, estimate to have higher active and/or lower side effect (for example in the intravital immunogenicity of people lower or do not have) being applied to man-hour.
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example, usually according to people such as normal condition such as Sambrook, molecular cloning: laboratory manual (New York:Cold Spring Harbor LaboratoryPress, 1989) condition described in, or the condition of advising according to manufacturer.Notice that in Nucleotide and amino acid composite sequence, (1) provides the position that initial sum stops first Nucleotide of coding, (2) molecular weight unit is dalton.
The acquisition of embodiment 1:cDNA gene and the restraining effect that the cancer cells clone is formed
LP4790, LP4791, LP4941, LP5085, LP5161, LP5852, LP5910, LP7057, LP9056 and LP9167 come from the normal hepatocytes cDNA library that makes up with ordinary method.Get normal liver tissue (LP clone), (GIBCO BRL company) extracts total RNA by manufacturer's specification sheets with Trizol reagent, extracts mRNA with the mRNA test kit (Pharmacia company) of purifying.Make up the cDNA library of above-mentioned mRNA with pCMV-script TMXR cDNA library construction test kit (Stratagene company).Wherein ThermoScript II is used MMLV-RT-Superscript II (GIBCO BRL) instead, and reverse transcription reaction carries out at 42 ℃.Transform the XL10-Gold recipient cell, obtained the cDNA library of 1 * 106cfu/ μ g cDNA titre.The first round is picking cDNA clone at random, is probe with high abundance cDNA clone with the cDNA clone who has proved cancer inhibitor cell growth function thereafter, screening by hybridization cDNA library, weak positive and negative clone of picking.With Qiagen 96 orifice plate plasmid extraction test kits, carry out the extraction of plasmid DNA by shop instruction.Plasmid DNA and empty carrier transfection simultaneously hepatoma cell line 7721.After the 100ng DNA alcohol precipitation drying, add 6 μ l H2O dissolving, treat transfection.Add 0.74 μ l liposome and 9.3 μ l serum-free mediums in every part of DNA sample, behind the mixing, room temperature was placed 10 minutes.Add 150 μ l serum-free mediums in every pipe, divide equally and add 3 holes and grow in 7721 cells of 96 orifice plates, placed 2 hours for 37 ℃, every hole adds 50 μ l serum-free mediums again, 37 ℃ 24 hours.Every hole is changed 100 μ l and is trained liquid entirely, 37 ℃ 24 hours, change the full training liquid 100 μ l that contain G418,37 ℃ 24-48 hour, the limit is observed, the training liquid that G418 concentration does not wait is changed on the limit.After about 2-3 time, there is the clone to form up to the microscopy cell, counting.Find that above-mentioned clone has the cell clone of inhibition formation effect, the result is as shown in the table.
CDNA clone's transfectional cell (7721) clone formation situation
| CDNA clones title | CDNA clones number (three repetitions) | Empty carrier clone number (three repetitions) | ||||
| ????LP4790 | ????1 | ????0 | ????2 | ????29 | ????30 | ????35 |
| ????LP4791 | ????1 | ????1 | ????0 | ????29 | ????30 | ????35 |
| ????LP4941 | ????1 | ????2 | ????1 | ????32 | ????20 | ????36 |
| ????LP5085 | ????0 | ????0 | ????0 | ????39 | ????44 | ????42 |
| ????LP5161 | ????0 | ????1 | ????1 | ????20 | ????18 | ????22 |
| ????LP5852 | ????3 | ????1 | ????1 | ????38 | ????32 | ????26 |
| ????LP5910 | ????1 | ????2 | ????0 | ????51 | ????29 | ????48 |
| ????LP7057 | ????0 | ????1 | ????4 | ????25 | ????22 | ????18 |
| ????LP9056 | ????1 | ????0 | ????0 | ????36 | ????40 | ????36 |
| ????LP9167 | ????0 | ????0 | ????0 | ????10 | ????13 | ????8 |
The cDNA clone is adopted two deoxidation cessation method, on the ABI377 automatic dna sequencer, measure the nucleotide sequence of the nearly 500bp of one end.After the analysis, be defined as novel gene cloning, carry out the other end order-checking, do not obtain full length cDNA sequence yet, the design primer checks order once more, up to obtaining full length sequence (SEQ ID NO:1,4,7,10,13,16,19,22,25,28).
Embodiment 2: PCR obtains full-length gene from liver cDNA:
Get normal liver tissue (LP clone), (GIBCO BRL company) extracts total RNA by manufacturer's specification sheets with Trizol reagent, extracts mRNA with the mRNA test kit (Pharmacia company) of purifying.With MMLV-RT-Superscript II (GIBCOBRL), ThermoScript II is carried out reverse transcription reaction at 42 ℃, obtains placenta cDNA.Utilize the special primer (as shown in the table) of each gene, by 97 ℃ of 3 ' 1 circulation.72 ℃ of 1 ' 35 circulations of 60 ℃ of 30 ∥ of 94 ℃ of 30 ∥, pcr amplification is carried out in 72 ℃ of 10 ' 1 circulations, and acquisition contains the amplified production of each protein gene of complete open reading frame sequence.Amplified production is through sequence verification, and the sequence that records with embodiment 1 conforms to, and changes amplified production over to host cell with routine techniques subsequently, obtains recombinant protein (SEQ ID NO:3,6,9,12,15,18,21,24,27,30).
Gene specific primer
| Clone's title | Special primer 1 (5 ' → 3 ') | ?SEQ?ID ???NO： | Special primer 2 (3 ' → 5 ') | ?SEQ?ID ???NO： |
| LP4790 | (3)GGCACCTCAGCAACCAGT | ???31 | CGAAACTAACTAAACCCGA(3584) | ???32 |
| LP4791 | (145)CAGTCTTCGCTCACTACAGCC | ???33 | CCAATCCAAAATGACCCCGT(2325) | ???34 |
| LP4941 | (15)AAGATGAATCCGTTGATCTCG | ???35 | TCGTTTGACAGGTCTTCCCT(2813) | ???36 |
| LP5085 | (139)AAGCCCTACAGTGCAGAGGA | ???37 | GAGAACCACAAAGTACGACG(2233) | ???38 |
| LP5161 | (61)GAAGAGGAGGAGGAAGAGGAG | ???39 | GTGGAAGGGACACTTTGTTC(3651) | ???40 |
| LP5852 | (66)CTTTCAGCTCGGCTGCTC | ???41 | GTACCTCATATCGCAGTAGGG(2587) | ???42 |
| LP5910 | (78)GAGGGTGCGGAGGACAAC | ???43 | CAAGGACGGTGACATGAGGT(3218) | ???44 |
| LP7057 | (59)CTTGTTCCTGCCGCTTTC | ???45 | TGTCTACTACTGACGTTTTACT(2579) | ???46 |
| LP9056 | (26)ATCGTCGGCCAGTTTATCC | ???47 | AATGGGGAACGACAAGTCTC(2682) | ???48 |
| LP9167 | (1)GGGAGGGCAGACTCTGGG | ???49 | AGTGCGGTACGGGTGGTG(2742) | ???50 |
Annotate: in the bracket is the correspondence position of primer in each gene DNA sequence.
Embodiment 3:cDNA cloned sequence is analyzed
1.LP4790
A: nucleotide sequence (SEQ ID NO:1) length: 3657 bases
B: aminoacid sequence (SEQ ID NO:3) length: 185 amino acid
C. Nucleotide and amino acid composite sequence (SEQ ID NO:2) clone number and protein name: LP4790
Start code: 2441 ATG stop coding: 2996 TAG protein molecular weights: 20778.75
2.LP4791
A: nucleotide sequence (SEQ ID NO:4) length: 2350 bases
B: aminoacid sequence (SEQ ID NO:6) length: 101 amino acid
C. Nucleotide and amino acid composite sequence (SEQ ID NO:5) clone number and protein name: LP4791
Start code: 647 ATG stop coding: 950 TAG protein molecular weights: 11200.29
3.LP4941
A: nucleotide sequence (SEQ ID NO:7) length: 2837 bases
B: aminoacid sequence (SEQ ID NO:9) length: 303 amino acid
C. Nucleotide and amino acid composite sequence (SEQ ID NO:8) clone number and protein name: LP4941
Start code: 134 ATG stop coding: 1043 TGA protein molecular weights: 34340.95
4.LP5085
A: nucleotide sequence (SEQ ID NO:10) length: 2280 bases
B: aminoacid sequence (SEQ ID NO:12) length: 148 amino acid
C. Nucleotide and amino acid composite sequence (SEQ ID NO:11) clone number and protein name: LP5085
Start code: 627 ATG stop coding: 1071 TAG protein molecular weights: 16212.66
5.LP5161
A: nucleotide sequence (SEQ ID NO:13) length: 3750 bases
B: aminoacid sequence (SEQ ID NO:15) length: 383 amino acid
C. Nucleotide and amino acid composite sequence (SEQ ID NO:14) clone number and protein name: LP5161
Start code: 688 ATG stop coding: 1837 TGA protein molecular weights: 42218.46
6.LP5852
A: nucleotide sequence (SEQ ID NO:16) length: 2730 bases
B: aminoacid sequence (SEQ ID NO:18) length: 101 amino acid
C. Nucleotide and amino acid composite sequence (SEQ ID NO.17) clone number and protein name: LP5852
Start code: 1216 ATG stop coding: 1519 TGA protein molecular weights: 10843.61
7.LP5910
A: nucleotide sequence (SEQ ID NO:19) length: 3260 bases
B: aminoacid sequence (SEQ ID NO:21) length: 187 amino acid
C. Nucleotide and amino acid composite sequence (SEQ ID NO:20) clone number and protein name: LP5910
Start code: 1310 ATG stop coding: 1871 TGA protein molecular weights: 19866.41
8.LP7057
A: nucleotide sequence (SEQ ID NO:22) length: 2692 bases
B: aminoacid sequence (SEQ ID NO:24) length: 113 amino acid
C. Nucleotide and amino acid composite sequence (SEQ ID NO:23) clone number and protein name: LP7057
Start code: 2238 ATG stop coding: 2577 TGA protein molecular weights: 12875.58
9.LP9056
A: nucleotide sequence (SEQ ID NO:25) length: 2769 bases
B: aminoacid sequence (SEQ ID NO:27) length: 563 amino acid
C. Nucleotide and amino acid composite sequence (SEQ ID NO:26) clone number and protein name: LP9056
Start code: 742 ATG stop coding: 2431 TGA protein molecular weights: 63543.64
10.LP9167
A: nucleotide sequence (SEQ ID NO:28) length: 3226 bases
B: aminoacid sequence (SEQ ID NO:30) length: 137 amino acid
C. Nucleotide and amino acid composite sequence (SEQ ID NO:29) clone number and protein name: LP9167
Start code: 38 ATG stop coding: 449 TGA protein molecular weights: 15172.09
All quote in this application as a reference at all documents that the present invention mentions, just quoted as a reference separately as each piece document.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after having read above-mentioned teachings of the present invention, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Sequence table
＜110〉Shanghai Xinshijie Gene Techn Development Co., Ltd.
＜120〉have new the people's albumen and the encoding sequence thereof of cancer suppressing function
<130>026810
<160>50
<170>PatentIn?version?3.1
<210>1
<211>3657
<212>DNA
＜213〉homo sapiens (Homo sapiens)
<400>1
ggggcacctc?agcaaccagt?agccatgcgc?ggcttggagg?agtcggggcc?tcggcctaca?????60
gcgaccccgt?gcggctgcgt?taagccggct?ctggagacag?ggaatctttt?aactgagcca????120
gtcggctact?tggaatcttg?tttctcggcc?aagaatggta?ctccaagaca?gccatccatt????180
tgtagctatt?ctcgagcctg?tttgaggatt?agaaagagga?tctttaataa?tcctgaacat????240
tccttgatgg?gcctagaaca?gtttttctca?tgtttggaag?gtctagtcat?gccaaatgga????300
aaaagaaagg?aaggtctggt?aaaggatttt?gtttgttttt?cacaaaaatg?gtcatttgag????360
ctgtaaggca?aaagtgcagc?ctcctaggct?gaatggtgca?aagactggag?ttttttccac????420
aaggagccct?catcgtccca?atgcaatagg?actgaccctg?gccaagctgg?aaaaggtaga????480
agccttgggg?aaatggacag?atatcatggc?tgcagtgctg?atgtcaactg?tgctgtgtgg????540
caggagtctg?ggatgaatca?cactacaagg?acaaacattg?gtcaggttgt?agaagtttta????600
aactagtttt?aactgtgtgt?tcgtatataa?cctagttacg?catttatgga?aagcattcca????660
ttctgaccta?acagatgcat?ttgttataga?ggcagcagat?gaaatggcag?gtaaactagg????720
agtcaagaga?ccattccagg?cttcagcctg?ctggttgtga?agtgggcaaa?ggagaaggac????780
ctgcttcctc?tgtgattcca?cagaatattt?tgagaatctt?gtaagagaga?agttctaaaa????840
ctactttttc?aggctattgg?acactaaata?gatgagagca?gttattttgc?ttgttctttc????900
ccagaacttg?aactctccca?ctggattgta?ctcaactggg?ccttaggtag?ttttctcaga????960
aaaattcagt?agctgatcca?ccttgaattt?tatcaatttg?taatggctaa?caaacttgga???1020
tagcaagtac?tagaagattt?tatgattatt?cagggacata?ttttctagca?ctgaatatgt???1080
gccagacatt?gtcctaggtg?ctagagctat?agtgataaac?aaatcagatg?aaagtagaaa???1140
tccatgccct?aaagaactta?atgtttcaat?tgggaaggtc?aagtcagata?ggttaaaagg???1200
caaacacagt?agcactgtag?acattactat?ataagcttta?tggcaactag?tgtttgtgca???1260
gtgctttaca?gttagtaaac?agtgttcacg?taccttatct?cacagtggct?ttgtgggttt???1320
gacaaagtag?caatcctcat?ctcataggtg?aggaaactag?ggctctttaa?gattagataa???1380
tctgtctaat?agcatccagc?caggtggtca?tctgactcta?aatcctttga?agtcttactt???1440
atgctacagt?gcctttcttt?ttcctacaga?tattgttagg?cataattcat?catctagcaa???1500
gtccagaatc?atctttccag?agttccagct?cattcataat?aacatgtttc?agaaatcagg???1560
gtcctgtatt?agttacgttt?ttcttaaatg?gagacggaat?caaatgtgac?tgcatgtagt???1620
ttttctgttc?tcatttggcc?tattttccaa?gagtaaggtt?agtagatggc?tatttgtagc???1680
agtaactgga?cagcatctgt?ccagtgggac?agctggagac?ttgtctttag?tatttggtaa???1740
tgctgactta?tgtgctgctt?aatcttttga?gtcttgatgg?atgtattaga?tttctattgt???1800
tgtgtaacaa?attaccacag?aatcatcagt?ttaaacaaca?cacatgtatt?atgtgtttga???1860
aacattatta?tgtatctgtg?ggtcagaagg?atgggcacag?cttaactagg?tcatctgctt???1920
aggtctcact?agactgcaat?taaattgtca?gctgatatga?gttcttatct?ggaggcttga???1980
ccatggaaga?atctgcttaa?gctcactcag?actgttggcc?acattgattt?ccttgccatc???2040
gtagtaggac?tgggggcccc?agattcttgc?tggttgttgg?ctggaggctg?ccctcagtgt???2100
ctaaaggctg?tttgcagttc?cttgctatgt?gggcttccta?gcacgcctgc?ctacttcagc???2160
aagccttcaa?ggagagtctc?tcgagtgagt?cttccagcca?gcagcagtgc?taatgaaaag???2220
catagtcact?ggagggacat?catacctctt?ttgccatatt?ctgttggtta?gaagccagtc???2280
acactcaagg?ggaaggatta?tataagggca?tgaacttcag?gaggtgcggg?tcacagggcc???2340
acttttggat?ctgtttgcca?cagtaagaac?cttcattggt?cccacagaga?tatactgagg???2400
actactatat?ccctggaaca?gaagcaccag?tgatacaaag?atgagttggt?cccagcctgt???2460
gttaaggggc?ctgcctgcgt?ctagggatga?agactctaag?caggtcacta?ccctaccgtg???2520
gggaaagcgc?tctagcagag?gtggagctat?atacctttct?ggaattgaca?tgatacatgg???2580
cacacccgta?ctagacatca?agccctacat?agctgagtat?gactcaccgc?aaaatgtgat???2640
ggagccttta?gcagacttta?atttacagaa?taaccaacat?acaccaaaca?ctgtgtccca???2700
gtctgacagc?aagactgaca?gctgtgacca?gcgacagctc?tcagggtgtg?atgagccaca???2760
accccaccat?agcactaaga?ggaaacctaa?atgtcctgaa?gacagaactt?cagaagaaaa???2820
ctacctgaca?cacagtgaca?cagccagaat?tcagcaagca?tttcctatgc?acagggagat???2880
agcagtggat?tttggtttgg?aatcaagacg?tgatcagagt?tccagcgtgg?cagaagaaca???2940
aattggccca?tattgcccag?agaagagctt?ttcagagaaa?ggtacagaca?gaagctagaa???3000
agagtggaag?gagcagcagt?cttgcaagga?agcagggcag?agacacagcc?catggcccct???3060
cactgccctg?ctggaagggc?tgatggagct?ccccgcagca?tggttcctgc?ctgggtgaca???3120
gaggctcctg?tggccacttt?agaagtgcgg?tttactcctc?atgccgagat?ggaccttggg???3180
cagctcagtt?cacaagatgt?tggtcaggcg?tcatttaaat?attttcagtc?agcagaggaa???3240
gcaaagcgtg?ccattgaggc?tgtgctgtca?gcggatcctc?ggtctgtgta?ccgccggaag???3300
ctttgccagg?accgcctttt?ctactttact?gtagacatag?cgcatgtcac?ttgctggttt???3360
ggtgatggct?ttgcagaggt?gctgaggatc?aagccggctt?ctgagcctgt?tcatatgact???3420
ggccctgtgg?ggtccttggt?gtctctaggg?tcttaaggag?cctccctcat?gtctttaagg???3480
tagcatcatt?gatctttgga?tgtggctttt?ggattttctg?aacaagctaa?tgttgtgtca???3540
agaagcaaca?ctttgtgatc?tcatggcttt?gattgatttg?ggctgttcaa?aatgtttatt???3600
tgaaaaacgt?ataccttaat?aaacttaaca?aagagatata?aaaaaaaaaa?aaaaaaa??????3657
<210>2
<211>3657
<212>DNA
＜213〉homo sapiens (Homo sapiens)
<220>
<221>CDS
<222>(2441)..(2995)
<223>
<400>2
ggggcacctc?agcaaccagt?agccatgcgc?ggcttggagg?agtcggggcc?tcggcctaca?????60
gcgaccccgt?gcggctgcgt?taagccggct?ctggagacag?ggaatctttt?aactgagcca????120
gtcggctact?tggaatcttg?tttctcggcc?aagaatggta?ctccaagaca?gccatccatt????180
tgtagctatt?ctcgagcctg?tttgaggatt?agaaagagga?tctttaataa?tcctgaacat????240
tccttgatgg?gcctagaaca?gtttttctca?tgtttggaag?gtctagtcat?gccaaatgga????300
aaaagaaagg?aaggtctggt?aaaggatttt?gtttgttttt?cacaaaaatg?gtcatttgag????360
ctgtaaggca?aaagtgcagc?ctcctaggct?gaatggtgca?aagactggag?ttttttccac????420
aaggagccct?catcgtccca?atgcaatagg?actgaccctg?gccaagctgg?aaaaggtaga????480
agccttgggg?aaatggacag?atatcatggc?tgcagtgctg?atgtcaactg?tgctgtgtgg????540
caggagtctg?ggatgaatca?cactacaagg?acaaacattg?gtcaggttgt?agaagtttta????600
aactagtttt?aactgtgtgt?tcgtatataa?cctagttacg?catttatgga?aagcattcca????660
ttctgaccta?acagatgcat?ttgttataga?ggcagcagat?gaaatggcag?gtaaactagg????720
agtcaagaga?ccattccagg?cttcagcctg?ctggttgtga?agtgggcaaa?ggagaaggac????780
ctgcttcctc?tgtgattcca?cagaatattt?tgagaatctt?gtaagagaga?agttctaaaa????840
ctactttttc?aggctattgg?acactaaata?gatgagagca?gttattttgc?ttgttctttc????900
ccagaacttg?aactctccca?ctggattgta?ctcaactggg?ccttaggtag?ttttctcaga????960
aaaattcagt?agctgatcca?ccttgaattt?tatcaatttg?taatggctaa?caaacttgga???1020
tagcaagtac?tagaagattt?tatgattatt?cagggacata?ttttctagca?ctgaatatgt???1080
gccagacatt?gtcctaggtg?ctagagctat?agtgataaac?aaatcagatg?aaagtagaaa???1140
tccatgccct?aaagaactta?atgtttcaat?tgggaaggtc?aagtcagata?ggttaaaagg???1200
caaacacagt?agcactgtag?acattactat?ataagcttta?tggcaactag?tgtttgtgca???1260
gtgctttaca?gttagtaaac?agtgttcacg?taccttatct?cacagtggct?ttgtgggttt???1320
gacaaagtag?caatcctcat?ctcataggtg?aggaaactag?ggctctttaa?gattagataa???1380
tctgtctaat?agcatccagc?caggtggtca?tctgactcta?aatcctttga?agtcttactt???1440
atgctacagt?gcctttcttt?ttcctacaga?tattgttagg?cataattcat?catctagcaa???1500
gtccagaatc?atctttccag?agttccagct?cattcataat?aacatgtttc?agaaatcagg???1560
gtcctgtatt?agttacgttt?ttcttaaatg?gagacggaat?caaatgtgac?tgcatgtagt???1620
ttttctgttc?tcatttggcc?tattttccaa?gagtaaggtt?agtagatggc?tatttgtagc???1680
agtaactgga?cagcatctgt?ccagtgggac?agctggagac?ttgtctttag?tatttggtaa???1740
tgctgactta?tgtgctgctt?aatcttttga?gtcttgatgg?atgtattaga?tttctattgt???1800
tgtgtaacaa?attaccacag?aatcatcagt?ttaaacaaca?cacatgtatt?atgtgtttga???1860
aacattatta?tgtatctgtg?ggtcagaagg?atgggcacag?cttaactagg?tcatctgctt???1920
aggtctcact?agactgcaat?taaattgtca?gctgatatga?gttcttatct?ggaggcttga???1980
ccatggaaga?atctgcttaa?gctcactcag?actgttggcc?acattgattt?ccttgccatc???2040
gtagtaggac?tgggggcccc?agattcttgc?tggttgttgg?ctggaggctg?ccctcagtgt???2100
ctaaaggctg?tttgcagttc?cttgctatgt?gggcttccta?gcacgcctgc?ctacttcagc???2160
aagccttcaa?ggagagtctc?tcgagtgagt?cttccagcca?gcagcagtgc?taatgaaaag???2220
catagtcact?ggagggacat?catacctctt?ttgccatatt?ctgttggtta?gaagccagtc???2280
acactcaagg?ggaaggatta?tataagggca?tgaacttcag?gaggtgcggg?tcacagggcc???2340
acttttggat?ctgtttgcca?cagtaagaac?cttcattggt?cccacagaga?tatactgagg???2400
actactatat?ccctggaaca?gaagcaccag?tgatacaaag?atg?agt?tgg?tcc?cag?????2455
Met?Ser?Trp?Ser?Gln
1???????????????5
cct?gtg?tta?agg?ggc?ctg?cct?gcg?tct?agg?gat?gaa?gac?tct?aag?cag?????2503
Pro?Val?Leu?Arg?Gly?Leu?Pro?Ala?Ser?Arg?Asp?Glu?Asp?Set?Lys?Gln
10??????????????????15??????????????????20
gtc?act?acc?cta?ccg?tgg?gga?aag?cgc?tct?agc?aga?ggt?gga?gct?ata?????2551
Val?Thr?Thr?Leu?Pro?Trp?Gly?Lys?Arg?Ser?Ser?Arg?Gly?Gly?Ala?Ile
25??????????????????30??????????????????35
tac?ctt?tct?gga?att?gac?atg?ata?cat?ggc?aca?ccc?gta?cta?gac?atc?????2599
Tyr?Leu?Ser?Gly?Ile?Asp?Met?Ile?His?Gly?Thr?Pro?Val?Leu?Asp?Ile
40??????????????????45??????????????????50
aag?ccc?tac?ata?gct?gag?tat?gac?tca?ccg?caa?aat?gtg?atg?gag?cct?????2647
Lys?Pro?Tyr?Ile?Ala?Glu?Tyr?Asp?Ser?Pro?Gln?Asn?Val?Met?Glu?Pro
55??????????????????60??????????????????65
tta?gca?gac?ttt?aat?tta?cag?aat?aac?caa?cat?aca?cca?aac?act?gtg?????2695
Leu?Ala?Asp?Phe?Asn?Leu?Gln?Asn?Asn?Gln?His?Thr?Pro?Asn?Thr?Val
70??????????????????75??????????????????80??????????????????85
tcc?cag?tct?gac?agc?aag?act?gac?agc?tgt?gac?cag?cga?cag?ctc?tca?????2743
Ser?Gln?Ser?Asp?Ser?Lys?Thr?Asp?Ser?Cys?Asp?Gln?Arg?Gln?Leu?Ser
90??????????????????95??????????????????100
ggg?tgt?gat?gag?cca?caa?ccc?cac?cat?agc?act?aag?agg?aaa?cct?aaa?????2791
Gly?Cys?Asp?Glu?Pro?Gln?Pro?His?His?Ser?Thr?Lys?Arg?Lys?Pro?Lys
105?????????????????110?????????????????115
tgt?cct?gaa?gac?aga?act?tca?gaa?gaa?aac?tac?ctg?aca?cac?agt?gac?????2839
Cys?Pro?Glu?Asp?Arg?Thr?Ser?Glu?Glu?Asn?Tyr?Leu?Thr?His?Ser?Asp
120?????????????????125?????????????????130
aca?gcc?aga?att?cag?caa?gca?ttt?cct?atg?cac?agg?gag?ata?gca?gtg?????2887
Thr?Ala?Arg?Ile?Gln?Gln?Ala?Phe?Pro?Met?His?Arg?Glu?Ile?Ala?Val
135?????????????????140?????????????????145
gat?ttt?ggt?ttg?gaa?tca?aga?cgt?gat?cag?agt?tcc?agc?gtg?gca?gaa?????2935
Asp?Phe?Gly?Leu?Glu?Ser?Arg?Arg?Asp?Gln?Ser?Ser?Ser?Val?Ala?Glu
150?????????????????155?????????????????160?????????????????165
gaa?caa?att?ggc?cca?tat?tgc?cca?gag?aag?agc?ttt?tca?gag?aaa?ggt?????2983
Glu?Gln?Ile?Gly?Pro?Tyr?Cys?Pro?Glu?Lys?Ser?Phe?Ser?Glu?Lys?Gly
170?????????????????175?????????????????180
aca?gac?aga?agc?tagaaagagt?ggaaggagca?gcagtcttgc?aaggaagcag?????????3035
Thr?Asp?Arg?Ser
185
ggcagagaca?cagcccatgg?cccctcactg?ccctgctgga?agggctgatg?gagctccccg???3095
cagcatggtt?cctgcctggg?tgacagaggc?tcctgtggcc?actttagaag?tgcggtttac???3155
tcctcatgcc?gagatggacc?ttgggcagct?cagttcacaa?gatgttggtc?aggcgtcatt???3215
taaatatttt?cagtcagcag?aggaagcaaa?gcgtgccatt?gaggctgtgc?tgtcagcgga???3275
tcctcggtct?gtgtaccgcc?ggaagctttg?ccaggaccgc?cttttctact?ttactgtaga???3335
catagcgcat?gtcacttgct?ggtttggtga?tggctttgca?gaggtgctga?ggatcaagcc??3395
ggcttctgag?cctgttcata?tgactggccc?tgtggggtcc?ttggtgtctc?tagggtctta??3455
aggagcctcc?ctcatgtctt?taaggtagca?tcattgatct?ttggatgtgg?cttttggatt??3515
ttctgaacaa?gctaatgttg?tgtcaagaag?caacactttg?tgatctcatg?gctttgattg??3575
atttgggctg?ttcaaaatgt?ttatttgaaa?aacgtatacc?ttaataaact?taacaaagag??3635
atataaaaaa?aaaaaaaaaa?aa???????????????????????????????????????????3657
<210>3
<211>185
<212>PRT
＜213〉homo sapiens (Homo sapiens)
<400>3
Met?Ser?Trp?Ser?Gln?Pro?Val?Leu?Arg?Gly?Leu?Pro?Ala?Ser?Arg?Asp
1???????????????5???????????????????10??????????????????15
Glu?Asp?Ser?Lys?Gln?Val?Thr?Thr?Leu?Pro?Trp?Gly?Lys?Arg?Ser?Ser
20??????????????????25??????????????????30
Arg?Gly?Gly?Ala?Ile?Tyr?Leu?Ser?Gly?Ile?Asp?Met?Ile?His?Gly?Thr
35??????????????????40??????????????????45
Pro?Val?Leu?Asp?Ile?Lys?Pro?Tyr?Ile?Ala?Glu?Tyr?Asp?Ser?Pro?Gln
50??????????????????55??????????????????60
Asn?Val?Met?Glu?Pro?Leu?Ala?Asp?Phe?Asn?Leu?Gln?Asn?Asn?Gln?His
65??????????????????70??????????????????75??????????????????80
Thr?Pro?Asn?Thr?Val?Ser?Gln?Ser?Asp?Ser?Lys?Thr?Asp?Ser?Cys?Asp
85??????????????????90??????????????????95
Gln?Arg?Gln?Leu?Ser?Gly?Cys?Asp?Glu?Pro?Gln?Pro?His?His?Ser?Thr
100?????????????????105?????????????????110
Lys?Arg?Lys?Pro?Lys?Cys?Pro?Glu?Asp?Arg?Thr?Ser?Glu?Glu?Asn?Tyr
115?????????????????120?????????????????125
Leu?Thr?His?Ser?Asp?Thr?Ala?Arg?Ile?Gln?Gln?Ala?Phe?Pro?Met?His
130?????????????????135?????????????????140
Arg?Glu?Ile?Ala?Val?Asp?Phe?Gly?Leu?Glu?Ser?Arg?Arg?Asp?Gln?Ser
145?????????????????150?????????????????155?????????????????160
Ser?Ser?Val?Ala?Glu?Glu?Gln?Ile?Gly?Pro?Tyr?Cys?Pro?Glu?Lys?Ser
165?????????????????170?????????????????175
Phe?Ser?Glu?Lys?Gly?Thr?Asp?Arg?Ser
180?????????????????185
<210>4
<211>2350
<212>DNA
＜213〉homo sapiens (Homo sapiens)
<400>4
gcgggcttac?atccaaatac?agatttaccc?cagaagagaa?tgaaacaatg?ttcagaaccc?????60
ctgaaaactg?tgttcaagga?agtcagctgg?atttttttga?gacaggggtc?ttgctcttgt????120
tgcccaggct?ggaatgcaat?ggtgcagtct?tcgctcacta?cagcctctac?cgctcctgct????180
caggtaatcc?ttccacctca?gccccctgag?tagctgggac?tgcaggcaca?cgccaccatg????240
ccaggctgat?ttttgtattt?tttgtagaga?ctgtgtttca?ccgtattgcc?cgggctggtc????300
ttgaactctt?ggactcaagc?aatccacctg?cctcggcctc?ccaaagtgct?cacaggcatg????360
aggcaacgtg?cccagcctca?tttgatactt?ttaaaataca?tttttaatat?ttttttcctc????420
caagatgcct?tccccattgt?gtaagtttca?ggtcttacaa?acctggatcc?accccagcag????480
tctgagttgc?atatatacat?acctcctatt?tacatgtccc?tcctcccact?attatgtcac????540
ctttctaaac?accgcaattg?ggagatgaga?caatgcgcag?gaacgaagaa?ctgttctggt????600
gaagaggacc?caggggcagt?gagagggggc?tagggacaaa?aggtgaatgg?ggagctggtt????660
caggttagaa?gaatgggtgc?atgtgagggg?catttggaga?atgcgagctg?ggggagggag????720
agtggctgag?cctgtaggca?cagtagacac?atgtagtaat?gacggggatg?tgttatgccc????780
cacaccaggt?gttgtcctac?atcaatgggg?tcacaacaag?caaacctgga?gtatccttgg????840
tctactccat?gccctcccgg?aacctgtccc?tgcggctgga?gggtctccag?gagaaagact????900
ctggccccta?cagctgctcc?gtgaatgtgc?aagacaaaca?aggcaaatct?aggggccaca????960
gcatcaaaac?cttagaactc?aatgtactgg?gtgagtgaga?agcagatttc?cggacccctc???1020
cccacctgca?ctgggaggtc?tggtgagtct?ctctcctaaa?tgacaaaagt?tggaggagga???1080
acaaatgaag?cgacagaagg?gtgcagggca?gagggagagg?atctgtgggt?ctcctgggtg???1140
gtgggtttta?atctgtctcc?ccctgcccag?ttcctccagc?tcctccatcc?tgccgtctcc???1200
agggtgtgcc?ccatgtgggg?gcaaacgtga?ccctgagctg?ccagtctcca?aggagtaagc???1260
ccgctgtcca?ataccagtgg?gatcggcagc?ttccatcctt?ccagactttc?tttgcaccag???1320
cattagatgt?catccgtggg?tctttaagcc?tcaccaacct?ttcgtcttcc?atggctggag???1380
tctatgtctg?caaggcccac?aatgaggtgg?gcactgccca?atgtaatgtg?acgctggaag???1440
tgagcacagg?tcagtgaggg?ggcctggagc?tgcagtggtt?gctggagctg?ttgtgggtac???1500
cctggttgga?ctggggttgc?tggctgggct?ggtcctcttg?taccaccgcc?ggggcaaggc???1560
cctggaggag?ccagccaatg?atatcaagga?ggatgccatt?gctccccgga?ccctgccctg???1620
gcccaagagc?tcagacacaa?tctccaagaa?tgggaccctt?tcctctgtca?cctccgcacg???1680
agccctccgg?ccaccccatg?gccctcccag?gcctggtgca?ttgaccccca?cgcccagtct???1740
ctccagccag?gccctgccct?caccaagact?gcccacgaca?gatggggccc?accctcaacc???1800
aatatccccc?atccctggtg?gggtttcttc?ctctggcttg?agccgcatgg?gtgctgtgcc???1860
tgtgatggtg?cctgcccaga?gtcaagctgg?ctctctggta?tgatgacccc?accactcatt???1920
ggctaaagga?tttggggtct?ctccttccta?taggggtcac?ctctagcaca?gaggcctgag???1980
tcatgggaaa?gagtcacact?cctgaccctt?agtactctgc?ccccacctct?ctttactgtg???2040
ggaaaaccat?ctcagtaaga?cctaagtgtc?caggagacag?aaggagaaga?ggaagtggat???2100
ctggaattgg?gaggagcctc?cacccacccc?tgactcctcc?ttatgaagcc?agctgctgaa???2160
attagctact?caccaagagt?gaggggcaga?gacttccagt?cactgagtct?cccaggcccc???2220
cttgatctgt?accccacccc?tatctaacac?cacccttggc?tcccactcca?gctccctgta???2280
ttgatataac?ctgtcaggct?ggcttggtta?ggttttactg?gggcagagga?tagggaatct???2340
cttattaaaa??????????????????????????????????????????????????????????2350
<210>5
<211>2350
<212>DNA
＜213〉homo sapiens (Homo sapiens)
<220>
<221>CDS
<222>(647)..(949)
<223>
<400>5
gcgggcttac?atccaaatac?agatttaccc?cagaagagaa?tgaaacaatg?ttcagaaccc?????60
ctgaaaactg?tgttcaagga?agtcagctgg?atttttttga?gacaggggtc?ttgctcttgt????120
tgcccaggct?ggaatgcaat?ggtgcagtct?tcgctcacta?cagcctctac?cgctcctgct????180
caggtaatcc?ttccacctca?gccccctgag?tagctgggac?tgcaggcaca?cgccaccatg????240
ccaggctgat?ttttgtattt?tttgtagaga?ctgtgtttca?ccgtattgcc?cgggctggtc????300
ttgaactctt?ggactcaagc?aatccacctg?cctcggcctc?ccaaagtgct?cacaggcatg????360
aggcaacgtg?cccagcctca?tttgatactt?ttaaaataca?tttttaatat?ttttttcctc????420
caagatgcct?tccccattgt?gtaagtttca?ggtcttacaa?acctggatcc?accccagcag????480
tctgagttgc?atatatacat?acctcctatt?tacatgtccc?tcctcccact?attatgtcac????540
ctttctaaac?accgcaattg?ggagatgaga?caatgcgcag?gaacgaagaa?ctgttctggt????600
gaagaggacc?caggggcagt?gagagggggc?tagggacaaa?aggtga?atg?ggg?agc???????655
Met?Gly?Ser
1
tgg?ttc?agg?tta?gaa?gaa?tgg?gtg?cat?gtg?agg?ggc?att?tgg?aga?atg??????703
Trp?Phe?Arg?Leu?Glu?Glu?Trp?Val?His?Val?Arg?Gly?Ile?Trp?Arg?Met
5???????????????????10??????????????????15
cga?gct?ggg?gga?ggg?aga?gtg?gct?gag?cct?gta?ggc?aca?gta?gac?aca??????751
Arg?Ala?Gly?Gly?Gly?Arg?Val?Ala?Glu?Pro?Val?Gly?Thr?Val?Asp?Thr
20??????????????????25??????????????????30??????????????????35
tgt?agt?aat?gac?ggg?gat?gtg?tta?tgc?ccc?aca?cca?ggt?gtt?gtc?cta??????799
Cys?Ser?Asn?Asp?Gly?Asp?Val?Leu?Cys?Pro?Thr?Pro?Gly?Val?Val?Leu
40??????????????????45??????????????????50
cat?caa?tgg?ggt?cac?aac?aag?caa?acc?tgg?agt?atc?ctt?ggt?cta?ctc??????847
His?Gln?Trp?Gly?His?Asn?Lys?Gln?Thr?Trp?Ser?Ile?Leu?Gly?Leu?Leu
55??????????????????60??????????????????65
cat?gcc?ctc?ccg?gaa?cct?gtc?cct?gcg?gct?gga?ggg?tct?cca?gga?gaa??????895
His?Ala?Leu?Pro?Glu?Pro?Val?Pro?Ala?Ala?Gly?Gly?Ser?Pro?Gly?Glu
70??????????????????75??????????????????80
aga?ctc?tgg?ccc?cta?cag?ctg?ctc?cgt?gaa?tgt?gca?aga?caa?aca?agg??????943
Arg?Leu?Trp?Pro?Leu?Gln?Leu?Leu?Arg?Glu?Cys?Ala?Arg?Gln?Thr?Arg
85??????????????????90??????????????????95
caa?atc?taggggccac?agcatcaaaa?ccttagaact?caatgtactg?ggtgagtgag???????999
Gln?Ile
100
aagcagattt?ccggacccct?ccccacctgc?actgggaggt?ctggtgagtc?tctctcctaa???1059
atgacaaaag?ttggaggagg?aacaaatgaa?gcgacagaag?ggtgcagggc?agagggagag???1119
gatctgtggg?tctcctgggt?ggtgggtttt?aatctgtctc?cccctgccca?gttcctccag???1179
ctcctccatc?ctgccgtctc?cagggtgtgc?cccatgtggg?ggcaaacgtg?accctgagct???1239
gccagtctcc?aaggagtaag?cccgctgtcc?aataccagtg?ggatcggcag?cttccatcct???1299
tccagacttt?ctttgcacca?gcattagatg?tcatccgtgg?gtctttaagc?ctcaccaacc???1359
tttcgtcttc?catggctgga?gtctatgtct?gcaaggccca?caatgaggtg?ggcactgccc???1419
aatgtaatgt?gacgctggaa?gtgagcacag?gtcagtgagg?gggcctggag?ctgcagtggt???1479
tgctggagct?gttgtgggta?ccctggttgg?actggggttg?ctggctgggc?tggtcctctt???1539
gtaccaccgc?cggggcaagg?ccctggagga?gccagccaat?gatatcaagg?aggatgccat???1599
tgctccccgg?accctgccct?ggcccaagag?ctcagacaca?atctccaaga?atgggaccct???1659
ttcctctgtc?acctccgcac?gagccctccg?gccaccccat?ggccctccca?ggcctggtgc???1719
attgaccccc?acgcccagtc?tctccagcca?ggccctgccc?tcaccaagac?tgcccacgac???1779
agatggggcc?caccctcaac?caatatcccc?catccctggt?ggggtttctt?cctctggctt???1839
gagccgcatg?ggtgctgtgc?ctgtgatggt?gcctgcccag?agtcaagctg?gctctctggt???1899
atgatgaccc?caccactcat?tggctaaagg?atttggggtc?tctccttcct?ataggggtca???1959
cctctagcac?agaggcctga?gtcatgggaa?agagtcacac?tcctgaccct?tagtactctg???2019
cccccacctc?tctttactgt?gggaaaacca?tctcagtaag?acctaagtgt?ccaggagaca???2079
gaaggagaag?aggaagtgga?tctggaattg?ggaggagcct?ccacccaccc?ctgactcctc???2139
cttatgaagc?cagctgctga?aattagctac?tcaccaagag?tgaggggcag?agacttccag???2199
tcactgagtc?tcccaggccc?ccttgatctg?taccccaccc?ctatctaaca?ccacccttgg???2259
ctcccactcc?agctccctgt?attgatataa?cctgtcaggc?tggcttggtt?aggttttact???2319
ggggcagagg?atagggaatc?tcttattaaa?a??????????????????????????????????2350
<210>6
<211>101
<212>PRT
＜213〉homo sapiens (Homo sapiens)
<400>6
Met?Gly?Ser?Trp?Phe?Arg?Leu?Glu?Glu?Trp?Val?His?Val?Arg?Gly?Ile
1???????????????5???????????????????10??????????????????15
Trp?Arg?Met?Arg?Ala?Gly?Gly?Gly?Arg?Val?Ala?Glu?Pro?Val?Gly?Thr
20??????????????????25??????????????????30
Val?Asp?Thr?Cys?Ser?Asn?Asp?Gly?Asp?Val?Leu?Cys?Pro?Thr?Pro?Gly
35??????????????????40??????????????????45
Val?Val?Leu?His?Gln?Trp?Gly?His?Asn?Lys?Gln?Thr?Trp?Ser?Ile?Leu
50??????????????????55??????????????????60
Gly?Leu?Leu?His?Ala?Leu?Pro?Glu?Pro?Val?Pro?Ala?Ala?Gly?Gly?Ser
65??????????????????70??????????????????75??????????????????80
Pro?Gly?Glu?Arg?Leu?Trp?Pro?Leu?Gln?Leu?Leu?Arg?Glu?Cys?Ala?Arg
85??????????????????90??????????????????95
Gln?Thr?Arg?Gln?Ile
100
<210>7
<211>2837
<212>DNA
＜213〉homo sapiens (Hono sapiens)
<400>7
ggtcgaggtg?tgcaaagatg?aatccgttga?tctcgaggaa?tttcgaagct?acctggagaa?????60
gcgttttgac?tttgagcaag?ttactgtgaa?aaaattcagg?acttgggctg?agcggcggca????120
attcaatcgg?gaaatgaagc?ggaagcaggc?ggagtccgag?aggcccatct?tgccagccaa????180
tcagaagctc?attactttat?cagtgcaaga?tgcacccaca?aagaaagagt?ttgttattaa????240
ccccaacggg?aaatccgagg?tctgcatcct?gcacgagtac?atgcagcgtg?tcctcaaggt????300
ccgccctgtc?tataatttct?ttgaatgtga?gaacccaagt?gagccttttg?gtgcctcggt????360
gaccattgat?ggtgtgactt?acggatctgg?aactgcaagc?agcaaaaaac?ttgcgaagaa????420
taaagctgcc?cgagctacac?tggaaatcct?catccctgac?tttgttaaac?agacctctga????480
agagaagccc?aaagacagtg?aagaactcga?gtattttaac?cacatcagca?tcgaggactc????540
gcgggtctac?gagctgacca?gcaaggctgg?gctgttgtct?ccatatcaga?tcctccacga????600
gtgccttaaa?agaaaccatg?ggatgggtga?cacgtctatc?aagtttgaag?tggttcctgg????660
gaaaaaccag?aagagtgaat?acgtcatggc?gtgtggcaag?cacacagtgc?gcgggtggtg????720
taagaacaag?agagttggaa?agcagttagc?ctcacagaag?atccttcagc?tgctgcaccc????780
acatgtcaag?aactgggggt?ctttactgcg?catgtatggc?cgtgagagca?gcaagatggt????840
caagcaggag?acatcggaca?agagtgtgat?tgagctgcag?cagtatgcca?agaagaacaa????900
gcccaacctg?cacatcctca?gcaagctcca?agaggagatg?aagaggctag?ctgaggaaag????960
ggaggagact?cgaaagaagc?ccaagatgtc?cattgtggcg?tccgcccagc?ctggcggtga???1020
gcccctgtgc?accgtggacg?tgtgagggag?gtggcacggg?ccagggcgcg?ggggccgcca???1080
gccgcacttc?tgaggagacc?agcagtcatg?catcgtgcac?cacagtgtca?ggcctccaac???1140
ccacgctcct?tccctgtggc?caacctgtgg?gcccggcctt?agggtggagg?ctttagtgta???1200
cagggacagc?catggccaca?cagcacacat?gtggagcagc?ggctctccct?ggaaagctcc???1260
aggcctgaat?ggatggactc?agcgactgca?ccagtggcag?ctggtgactg?tggacagtgg???1320
tggaccctgc?ttctgtgcac?ctgctgcagg?ctctttttat?gaaggctttc?atgaatttta???1380
gtatgtaata?cgcactgacg?acacatgatg?cttggatgac?agatgagagg?ggatggctga???1440
gtcctgtggc?tggcccgtga?tgccaggtgg?cccatgtgcc?cagggcgcct?gcagggctgc???1500
tacagggacc?tggtcaggag?gtgcacatgg?tgccctgccc?tcacccaccc?tctgtgtttc???1560
cccttctttg?aaaaggtaga?agagaaagga?atattttaaa?cctttttggc?ttaaacagaa???1620
ttttagcatc?agaactagct?ttctgggatt?ggaggcaaac?catcaaggtg?gtccctctcc???1680
agtctggaca?cgatgccagc?aaggatgacg?tcctgccacc?tcctggagtt?accctggcct???1740
cctagggtcc?ctttttctga?tgaagtctta?attccctaaa?agcgcctctt?tggacactga???1800
ggccctctct?gcctttcctg?gcctccggca?acagtttttt?acaaagattt?tttgcagtcg???1860
agtccatatg?tccacccatt?gatttttaaa?gcttttgtga?tattttagca?ttttgaaaga???1920
ctttcacagt?gagagtagaa?ggtagatttg?gaatcatgca?ttttagcaag?tggacttgtt???1980
gaaacaggaa?gcaagagcct?tcagtgtagc?ccattcttga?tccagagctg?ttgcctgtga???2040
cagcggtttc?tctggatgtc?aaaggcagct?gcctggtgcc?cagcttgctt?ctcgactggt???2100
ggcccctatg?ggtgggtgtg?cgatggaaat?gtgttcctgc?cggagtctga?ggcaccaggg???2160
tgtgctcaaa?ggctggccct?ggtggtggac?tggcacctgt?gcagagtgcc?gtgtgcttgt???2220
ggtgcgccat?ctgaagcaag?agtccagcgt?tctgccgtgt?ctgtccccca?ccatgccccc???2280
tacaggcggt?actgatggcg?cttttttttt?ttttttttct?gtcaggaaaa?caatgttggc???2340
ctgtgggccg?cccacaacat?atccttccct?cactacctgt?gtgaccaagg?ttggcttctg???2400
ttgaccttta?aaaaagaaac?cctcaactca?aattgctata?attagacact?tgcttctgtc???2460
ttgcctcctg?tctgcagctg?tgaatagtca?tttgactgtg?actgttgccc?ttagccagcc???2520
agatgcgcct?gtgaaccaaa?gctttgtgca?catgtgttcc?cctaaaggtt?ggggagcctc???2580
gctgtgtttt?gctgttccca?ggcaccacca?cagcaggtgc?tgccatactc?ttgtggtctc???2640
tgtgcgcccc?ccccccccca?cccgtctgcc?aagcatgggt?atgaatcgtg?cacacagcca???2700
tgcttcaagg?ccggggcagg?ggagcctgtg?ctgatgccat?ccagggcact?gggctgtgcc???2760
tggaaggcga?gccttgattg?tctgaacaca?taaagcaaac?tgtccagaag?ggaaaaaaaa???2820
aaaaaaaaaa?aaaaaaa??????????????????????????????????????????????????2837
<210>8
<211>2837
<212>DNA
＜213〉homo sapiens (Homo sapiens)
<220>
<221>CDS
<222>(134)..(1042)
<223>
<400>8
ggtcgaggtg?tgcaaagatg?aatccgttga?tctcgaggaa?tttcgaagct?acctggagaa?????60
gcgttttgac?tttgagcaag?ttactgtgaa?aaaattcagg?acttgggctg?agcggcggca????120
attcaatcgg?gaa?atg?aag?cgg?aag?cag?gcg?gag?tcc?gag?agg?ccc?atc???????169
Met?Lys?Arg?Lys?Gln?Ala?Glu?Ser?Glu?Arg?Pro?Ile
1???????????????5???????????????????10
ttg?cca?gcc?aat?cag?aag?ctc?att?act?tta?tca?gtg?caa?gat?gca?ccc??????217
Leu?Pro?Ala?Asn?Gln?Lys?Leu?Ile?Thr?Leu?Ser?Val?Gln?Asp?Ala?Pro
15??????????????????20??????????????????25
aca?aag?aaa?gag?ttt?gtt?att?aac?ccc?aac?ggg?aaa?tcc?gag?gtc?tgc??????265
Thr?Lys?Lys?Glu?Phe?Val?Ile?Asn?Pro?Asn?Gly?Lys?Ser?Glu?Val?Cys
30??????????????????35??????????????????40
atc?ctg?cac?gag?tac?atg?cag?cgt?gtc?ctc?aag?gtc?cgc?cct?gtc?tat??????313
Ile?Leu?His?Glu?Tyr?Met?Gln?Arg?Val?Leu?Lys?Val?Arg?Pro?Val?Tyr
45??????????????????50??????????????????55??????????????????60
aat?ttc?ttt?gaa?tgt?gag?aac?cca?agt?gag?cct?ttt?ggt?gcc?tcg?gtg??????361
Asn?Phe?Phe?Glu?Cys?Glu?Asn?Pro?Ser?Glu?Pro?Phe?Gly?Ala?Ser?Val
65??????????????????70??????????????????75
acc?att?gat?ggt?gtg?act?tac?gga?tct?gga?act?gca?agc?agc?aaa?aaa??????409
Thr?Ile?Asp?Gly?Val?Thr?Tyr?Gly?Ser?Gly?Thr?Ala?Ser?Ser?Lys?Lys
80??????????????????85??????????????????90
ctt?gcg?aag?aat?aaa?gct?gcc?cga?gct?aca?ctg?gaa?atc?ctc?atc?cct??????457
Leu?Ala?Lys?Asn?Lys?Ala?Ala?Arg?Ala?Thr?Leu?Glu?Ile?Leu?Ile?Pro
95??????????????????100?????????????????105
gac?ttt?gtt?aaa?cag?acc?tct?gaa?gag?aag?ccc?aaa?gac?agt?gaa?gaa??????505
Asp?Phe?Val?Lys?Gln?Thr?Ser?Glu?Glu?Lys?Pro?Lys?Asp?Ser?Glu?Glu
110?????????????????115?????????????????120
ctc?gag?tat?ttt?aac?cac?atc?agc?atc?gag?gac?tcg?cgg?gtc?tac?gag??????553
Leu?Glu?Tyr?Phe?Asn?His?Ile?Ser?Ile?Glu?Asp?Ser?Arg?Val?Tyr?Glu
125?????????????????130?????????????????135?????????????????140
ctg?acc?agc?aag?gct?ggg?ctg?ttg?tct?cca?tat?cag?atc?ctc?cac?gag??????601
Leu?Thr?Ser?Lys?Ala?Gly?Leu?Leu?Ser?Pro?Tyr?Gln?Ile?Leu?His?Glu
145?????????????????150?????????????????155
tgc?ctt?aaa?aga?aac?cat?ggg?atg?ggt?gac?acg?tct?atc?aag?ttt?gaa??????649
Cys?Leu?Lys?Arg?Asn?His?Gly?Met?Gly?Asp?Thr?Ser?Ile?Lys?Phe?Glu
160?????????????????165?????????????????170
gtg?gtt?cct?ggg?aaa?aac?cag?aag?agt?gaa?tac?gtc?atg?gcg?tgt?ggc??????697
Val?Val?Pro?Gly?Lys?Asn?Gln?Lys?Ser?Glu?Tyr?Val?Met?Ala?Cys?Gly
175?????????????????180?????????????????185
aag?cac?aca?gtg?cgc?ggg?tgg?tgt?aag?aac?aag?aga?gtt?gga?aag?cag??????745
Lys?His?Thr?Val?Arg?Gly?Trp?Cys?Lys?Asn?Lys?Arg?Val?Gly?Lys?Gln
190?????????????????195?????????????????200
tta?gcc?tca?cag?aag?atc?ctt?cag?ctg?ctg?cac?cca?cat?gtc?aag?aac??????793
Leu?Ala?Ser?Gln?Lys?Ile?Leu?Gln?Leu?Leu?His?Pro?His?Val?Lys?Asn
205?????????????????210?????????????????215?????????????????220
tgg?ggg?tct?tta?ctg?cgc?atg?tat?ggc?cgt?gag?agc?agc?aag?atg?gtc??????841
Trp?Gly?Ser?Leu?Leu?Arg?Met?Tyr?Gly?Arg?Glu?Ser?Ser?Lys?Met?Val
225?????????????????230?????????????????235
aag?cag?gag?aca?tcg?gac?aag?agt?gtg?att?gag?ctg?cag?cag?tat?gcc??????889
Lys?Gln?Glu?Thr?Ser?Asp?Lys?Ser?Val?Ile?Glu?Leu?Gln?Gln?Tyr?Ala
240?????????????????245?????????????????250
aag?aag?aac?aag?ccc?aac?ctg?cac?atc?ctc?agc?aag?ctc?caa?gag?gag??????937
Lys?Lys?Asn?Lys?Pro?Asn?Leu?His?Ile?Leu?Ser?Lys?Leu?Gln?Glu?Glu
255?????????????????260?????????????????265
atg?aag?agg?cta?gct?gag?gaa?agg?gag?gag?act?cga?aag?aag?ccc?aag??????985
Met?Lys?Arg?Leu?Ala?Glu?Glu?Arg?Glu?Glu?Thr?Arg?Lys?Lys?Pro?Lys
270?????????????????275?????????????????280
atg?tcc?att?gtg?gcg?tcc?gcc?cag?cct?ggc?ggt?gag?ccc?ctg?tgc?acc?????1033
Met?Ser?Ile?Val?Ala?Ser?Ala?Gln?Pro?Gly?Gly?Glu?Pro?Leu?Cys?Thr
285?????????????????290?????????????????295?????????????????300
gtg?gac?gtg?tgagggaggt?ggcacgggcc?agggcgcggg?ggccgccagc?????????????1082
Val?Asp?Val
cgcacttctg?aggagaccag?cagtcatgca?tcgtgcacca?cagtgtcagg?cctccaaccc???1142
acgctccttc?cctgtggcca?acctgtgggc?ccggccttag?ggtggaggct?ttagtgtaca???1202
gggacagcca?tggccacaca?gcacacatgt?ggagcagcgg?ctctccctgg?aaagctccag???1262
gcctgaatgg?atggactcag?cgactgcacc?agtggcagct?ggtgactgtg?gacagtggtg???1322
gaccctgctt?ctgtgcacct?gctgcaggct?ctttttatga?aggctttcat?gaattttagt???1382
atgtaatacg?cactgacgac?acatgatgct?tggatgacag?atgagagggg?atggctgagt???1442
cctgtggctg?gcccgtgatg?ccaggtggcc?catgtgccca?gggcgcctgc?agggctgcta???1502
cagggacctg?gtcaggaggt?gcacatggtg?ccctgccctc?acccaccctc?tgtgtttccc???1562
cttctttgaa?aaggtagaag?agaaaggaat?attttaaacc?tttttggctt?aaacagaatt???1622
ttagcatcag?aactagcttt?ctgggattgg?aggcaaacca?tcaaggtggt?ccctctccag???1682
tctggacacg?atgccagcaa?ggatgacgtc?ctgccacctc?ctggagttac?cctggcctcc???1742
tagggtccct?ttttctgatg?aagtcttaat?tccctaaaag?cgcctctttg?gacactgagg???1802
ccctctctgc?ctttcctggc?ctccggcaac?agttttttac?aaagattttt?tgcagtcgag???1862
tccatatgtc?cacccattga?tttttaaagc?ttttgtgata?ttttagcatt?ttgaaagact???1922
ttcacagtga?gagtagaagg?tagatttgga?atcatgcatt?ttagcaagtg?gacttgttga???1982
aacaggaagc?aagagccttc?agtgtagccc?attcttgatc?cagagctgtt?gcctgtgaca???2042
gcggtttctc?tggatgtcaa?aggcagctgc?ctggtgccca?gcttgcttct?cgactggtgg???2102
cccctatggg?tgggtgtgcg?atggaaatgt?gttcctgccg?gagtctgagg?caccagggtg???2162
tgctcaaagg?ctggccctgg?tggtggactg?gcacctgtgc?agagtgccgt?gtgcttgtgg???2222
tgcgccatct?gaagcaagag?tccagcgttc?tgccgtgtct?gtcccccacc?atgcccccta???2282
caggcggtac?tgatggcgct?tttttttttt?ttttttctgt?caggaaaaca?atgttggcct???2342
gtgggccgcc?cacaacatat?ccttccctca?ctacctgtgt?gaccaaggtt?ggcttctgtt???2402
gacctttaaa?aaagaaaccc?tcaactcaaa?ttgctataat?tagacacttg?cttctgtctt???2462
gcctcctgtc?tgcagctgtg?aatagtcatt?tgactgtgac?tgttgccctt?agccagccag???2522
atgcgcctgt?gaaccaaagc?tttgtgcaca?tgtgttcccc?taaaggttgg?ggagcctcgc???2582
tgtgttttgc?tgttcccagg?caccaccaca?gcaggtgctg?ccatactctt?gtggtctctg???2642
tgcgcccccc?cccccccacc?cgtctgccaa?gcatgggtat?gaatcgtgca?cacagccatg???2702
cttcaaggcc?ggggcagggg?agcctgtgct?gatgccatcc?agggcactgg?gctgtgcctg???2762
gaaggcgagc?cttgattgtc?tgaacacata?aagcaaactg?tccagaaggg?aaaaaaaaaa???2822
aaaaaaaaaa?aaaaa????????????????????????????????????????????????????2837
<210>9
<211>303
<212>PRT
＜213〉homo sapiens (Homo sapiens)
<400>9
Met?Lys?Arg?Lys?Gln?Ala?Glu?Ser?Glu?Arg?Pro?Ile?Leu?Pro?Ala?Asn
1???????????????5???????????????????10??????????????????15
Gln?Lys?Leu?Ile?Thr?Leu?Set?Val?Gln?Asp?Ala?Pro?Thr?Lys?Lys?Glu
20??????????????????25??????????????????30
Phe?Val?Ile?Asn?Pro?Asn?Gly?Lys?Ser?Glu?Val?Cys?Ile?Leu?His?Glu
35??????????????????40??????????????????45
Tyr?Met?Gln?Arg?Val?Leu?Lys?Val?Arg?Pro?Val?Tyr?Asn?Phe?Phe?Glu
50??????????????????55??????????????????60
Cys?Glu?Asn?Pro?Ser?Glu?Pro?Phe?Gly?Ala?Ser?Val?Thr?Ile?Asp?Gly
65??????????????????70??????????????????75??????????????????80
Val?Thr?Tyr?Gly?Ser?Gly?Thr?Ala?Ser?Ser?Lys?Lys?Leu?Ala?Lys?Asn
85??????????????????90??????????????????95
Lys?Ala?Ala?Arg?Ala?Thr?Leu?Glu?Ile?Leu?Ile?Pro?Asp?Phe?Val?Lys
100?????????????????105?????????????????110
Gln?Thr?Ser?Glu?Glu?Lys?Pro?Lys?Asp?Ser?Glu?Glu?Leu?Glu?Tyr?Phe
115?????????????????120?????????????????125
Asn?His?Ile?Ser?Ile?Glu?Asp?Ser?Arg?Val?Tyr?Glu?Leu?Thr?Ser?Lys
130?????????????????135?????????????????140
Ala?Gly?Leu?Leu?Ser?Pro?Tyr?Gln?Ile?Leu?His?Glu?Cys?Leu?Lys?Arg
145?????????????????150?????????????????155?????????????????160
Asn?His?Gly?Met?Gly?Asp?Thr?Ser?Ile?Lys?Phe?Glu?Val?Val?Pro?Gly
165?????????????????170?????????????????175
Lys?Asn?Gln?Lys?Ser?Glu?Tyr?Val?Met?Ala?Cys?Gly?Lys?His?Thr?Val
180?????????????????185?????????????????190
Arg?Gly?Trp?Cys?Lys?Asn?Lys?Arg?Val?Gly?Lys?Gln?Leu?Ala?Ser?Gln
195?????????????????200?????????????????205
Lys?Ile?Leu?Gln?Leu?Leu?His?Pro?His?Val?Lys?Asn?Trp?Gly?Ser?Leu
210?????????????????215?????????????????220
Leu?Arg?Met?Tyr?Gly?Arg?Glu?Ser?Ser?Lys?Met?Val?Lys?Gln?Glu?Thr
225?????????????????230?????????????????235?????????????????240
Ser?Asp?Lys?Ser?Val?Ile?Glu?Leu?Gln?Gln?Tyr?Ala?Lys?Lys?Asn?Lys
245?????????????????250?????????????????255
Pro?Asn?Leu?His?Ile?Leu?Ser?Lys?Leu?Gln?Glu?Glu?Met?Lys?Arg?Leu
260?????????????????265?????????????????270
Ala?Glu?Glu?Arg?Glu?Glu?Thr?Arg?Lys?Lys?Pro?Lys?Met?Ser?Ile?Val
275?????????????????280?????????????????285
Ala?Ser?Ala?Gln?Pro?Gly?Gly?Glu?Pro?Leu?Cys?Thr?Val?Asp?Val
290?????????????????295?????????????????300
<210>10
<211>2280
<212>DNA
＜213〉homo sapiens (Homo sapiens)
<400>10
gcgtctttta?cagaatgtgg?gatcctcgag?ctaagatgag?ggcatccctc?acgttcacac?????60
ccctggtggc?atctgccagc?cctgttctgg?ggacaaggcg?ggctttcgtg?ggagccatgc????120
tcagcctgcc?aggaagccaa?gccctacagt?gcagaggaaa?cagaatttca?acgggaagct????180
ggtttgcttc?ataccattgg?gatctgctgg?taaagctgtt?atttgggttt?agggactgat????240
cccttgcagt?ttacttctgg?atcaccatga?atggccaaga?tggtggcaga?acacgctgtg????300
gaccctgagt?tagagacaat?gcaaatgttg?gattgggtgt?aattcttttt?gaatcccaga????360
tccagtctgt?acttgaatat?gagcagaaga?tctacaagaa?tgctgacagg?gaaccgtgtt????420
aagacccagc?acccctattc?ccaggagctt?ctggcctgac?catctgcagc?caaagcacta????480
acagggacag?atatgggaat?gtccaccttt?gatccgcatc?ctgcacaata?gtggtcccac????540
catggctgcc?acttttttat?actatttgga?gaaaagacct?tgtataaatt?cgaggcccga????600
gtgactaacg?tctctgtcac?acggaaatgg?gtacttggtg?gcatagagaa?acacaattag????660
ccactttttc?agctacactt?ctcactcagc?tgcaccctac?acttctcact?caggtgcacc????720
cccttctgct?gtcctttccc?caacgtactg?ggtcccgagc?gtggtgggta?tttgccacac????780
tgggtgccag?ctcagcagcc?ccccacctct?ctttattctc?tccaaagctg?gtctttctga????840
ctatcattgt?ggtaggggga?ggacagatgc?taaaggtgga?agctgacctg?gagaaagaga????900
cacacggggt?gactgtggca?aaggacagct?ggaaaagaaa?ctctatcact?tcttcattgg????960
caaccacaag?gcacccgagg?ccatggcact?cccagaggct?gtgcgcagag?ccaagcctct???1020
caacctcttc?tggccctgcg?tcctgcagcg?aagtctctgc?tgtaagacag?tagactcctt???1080
cgatgaggtg?ctcaaaaatg?ctacccgggg?tggtggtgtc?tggcttgcag?tctggcccag???1140
ttcagagaaa?gttgcagaga?tcaggggcca?aggatgtcat?agccccaggt?tgtcctcagg???1200
gtcgcaatcc?tagggcaggg?tgtgcatgga?agcaagaact?atggaaacct?agctccagtc???1260
tgcaggctct?gagcccctag?ttcctcactc?cagcggggct?ccctcactgc?acagaaccca???1320
ccccttctgt?gtgggcactg?ctgaccacac?agatgaccca?gacccaaaga?gcctggcaga???1380
agctctgtgg?ttggagctgg?gctccgtctc?caggtctggt?tcagggggat?caggaaggct???1440
cttttccacc?tgtggcttca?ctggcccttt?gagatttcct?atctcaccgt?tacttcagtt???1500
acccttgcag?ggggccaggg?agtcaagaat?ataccgtgtt?cctccagggt?ttaagccggc???1560
catgccttcc?cgagagcata?accaacttga?caggggtgcc?cagttacccc?acaaactgaa???1620
ggaaggagat?ccttcccccg?tccccaggag?tgctctcaac?cagcctcaga?aagcttgaga???1680
agatggaccc?tttgcccacc?agggttaatt?cctggtgggg?cagctcggct?gtgatcaggg???1740
caaccaaacc?tataggaagc?cttccagtgt?gagctggaat?tagactgaac?atgtgcttgg???1800
gcctgcctct?ccctagacgc?agttgcgggg?cactccaggg?aatgaaccag?ctcaagtgtg???1860
tccctaacag?cagcctggag?ctacccccaa?tccctcacag?cctgaccctc?ctcattccat???1920
cagatgcatt?tgtagaatcg?gggcaaattt?ccttatttat?ttatggccca?tgcctttccc???1980
cctcttcatc?ctgatcccgt?tttgctttga?agagacccca?gtaaccaaaa?aacagcctcc???2040
agaagccaaa?accatgcctg?gatctcccat?agcttctcct?ttgcttccag?gagaaagttc???2100
actgaaaaaa?aaatatcttc?tggcttcttg?tgtgtacaga?gacaacagaa?ctcggtgggg???2160
aaacgggaat?cttttctgca?ccaaagctgc?ttctaaagca?gaaagcagtg?gggctcttgg???2220
tgtttcatgc?tgccttattt?atattaaagg?aagaattaaa?tcttaaaaaa?aaaaaaaaaa???2280
<210>11
<211>2280
<212>DNA
＜213〉homo sapiens (Homo sapiens)
<220>
<221>CDS
<222>(627)..(1070)
<223>
<400>11
gcgtctttta?cagaatgtgg?gatcctcgag?ctaagatgag?ggcatccctc?acgttcacac???60
ccctggtggc?atctgccagc?cctgttctgg?ggacaaggcg?ggctttcgtg?ggagccatgc??120
tcagcctgcc?aggaagccaa?gccctacagt?gcagaggaaa?cagaatttca?acgggaagct??180
ggtttgcttc?ataccattgg?gatctgctgg?taaagctgtt?atttgggttt?agggactgat??240
cccttgcagt?ttacttctgg?atcaccatga?atggccaaga?tggtggcaga?acacgctgtg????300
gaccctgagt?tagagacaat?gcaaatgttg?gattgggtgt?aattcttttt?gaatcccaga????360
tccagtctgt?acttgaatat?gagcagaaga?tctacaagaa?tgctgacagg?gaaccgtgtt????420
aagacccagc?acccctattc?ccaggagctt?ctggcctgac?catctgcagc?caaagcacta????480
acagggacag?atatgggaat?gtccaccttt?gatccgcatc?ctgcacaata?gtggtcccac????540
catggctgcc?acttttttat?actatttgga?gaaaagacct?tgtataaatt?cgaggcccga????600
gtgactaacg?tctctgtcac?acggaa?atg?ggt?act?tgg?tgg?cat?aga?gaa?aca?????653
Met?Gly?Thr?Trp?Trp?His?Arg?Glu?Thr
1???????????????5
caa?tta?gcc?act?ttt?tca?gct?aca?ctt?ctc?act?cag?ctg?cac?cct?aca??????701
Gln?Leu?Ala?Thr?Phe?Ser?Ala?Thr?Leu?Leu?Thr?Gln?Leu?His?Pro?Thr
10??????????????????15??????????????????20??????????????????25
ctt?ctc?act?cag?gtg?cac?ccc?ctt?ctg?ctg?tcc?ttt?ccc?caa?cgt?act??????749
Leu?Leu?Thr?Gln?Val?His?Pro?Leu?Leu?Leu?Ser?Phe?Pro?Gln?Arg?Thr
30??????????????????35??????????????????40
ggg?tcc?cga?gcg?tgg?tgg?gta?ttt?gcc?aca?ctg?ggt?gcc?agc?tca?gca??????797
Gly?Ser?Arg?Ala?Trp?Trp?Val?Phe?Ala?Thr?Leu?Gly?Ala?Ser?Ser?Ala
45??????????????????50??????????????????55
gcc?ccc?cac?ctc?tct?tta?ttc?tct?cca?aag?ctg?gtc?ttt?ctg?act?atc??????845
Ala?Pro?His?Leu?Ser?Leu?Phe?Ser?Pro?Lys?Leu?Val?Phe?Leu?Thr?Ile
60??????????????????65??????????????????70
att?gtg?gta?ggg?gga?gga?cag?atg?cta?aag?gtg?gaa?gct?gac?ctg?gag??????893
Ile?Val?Val?Gly?Gly?Gly?Gln?Met?Leu?Lys?Val?Glu?Ala?Asp?Leu?Glu
75??????????????????80??????????????????85
aaa?gag?aca?cac?ggg?gtg?act?gtg?gca?aag?gac?agc?tgg?aaa?aga?aac??????941
Lys?Glu?Thr?His?Gly?Val?Thr?Val?Ala?Lys?Asp?Ser?Trp?Lys?Arg?Asn
90??????????????????95??????????????????100?????????????????105
tct?atc?act?tct?tca?ttg?gca?acc?aca?agg?cac?ccg?agg?cca?tgg?cac??????989
Ser?Ile?Thr?Ser?Ser?Leu?Ala?Thr?Thr?Arg?His?Pro?Arg?Pro?Trp?His
110?????????????????115?????????????????120
tcc?cag?agg?ctg?tgc?gca?gag?cca?agc?ctc?tca?acc?tct?tct?ggc?cct?????1037
Ser?Gln?Arg?Leu?Cys?Ala?Glu?Pro?Ser?Leu?Ser?Thr?Ser?Ser?Gly?Pro
125?????????????????t30?????????????????135
gcg?tcc?tgc?agc?gaa?gtc?tct?gct?gta?aga?cag?tagactcctt?cgatgaggtg???1090
Ala?Ser?Cys?Ser?Glu?Val?Ser?Ala?Val?Arg?Gln
140?????????????????145
ctcaaaaatg?ctacccgggg?tggtggtgtc?tggcttgcag?tctggcccag?ttcagagaaa???1150
gttgcagaga?tcaggggcca?aggatgtcat?agccccaggt?tgtcctcagg?gtcgcaatcc???1210
tagggcaggg?tgtgcatgga?agcaagaact?atggaaacct?agctccagtc?tgcaggctct???1270
gagcccctag?ttcctcactc?cagcggggct?ccctcactgc?acagaaccca?ccccttctgt???1330
gtgggcactg?ctgaccacac?agatgaccca?gacccaaaga?gcctggcaga?agctctgtgg???1390
ttggagctgg?gctccgtctc?caggtctggt?tcagggggat?caggaaggct?cttttccacc???1450
tgtggcttca?ctggcccttt?gagatttcct?atctcaccgt?tacttcagtt?acccttgcag???1510
ggggccaggg?agtcaagaat?ataccgtgtt?cctccagggt?ttaagccggc?catgccttcc???1570
cgagagcata?accaacttga?caggggtgcc?cagttacccc?acaaactgaa?ggaaggagat???1630
ccttcccccg?tccccaggag?tgctctcaac?cagcctcaga?aagcttgaga?agatggaccc???1690
tttgcccacc?agggttaatt?cctggtgggg?cagctcggct?gtgatcaggg?caaccaaacc???1750
tataggaagc?cttccagtgt?gagctggaat?tagactgaac?atgtgcttgg?gcctgcctct???1810
ccctagacgc?agttgcgggg?cactccaggg?aatgaaccag?ctcaagtgtg?tccctaacag???1870
cagcctggag?ctacccccaa?tccctcacag?cctgaccctc?ctcattccat?cagatgcatt???1930
tgtagaatcg?gggcaaattt?ccttatttat?ttatggccca?tgcctttccc?cctcttcatc???1990
ctgatcccgt?tttgctttga?agagacccca?gtaaccaaaa?aacagcctcc?agaagccaaa???2050
accatgcctg?gatctcccat?agcttctcct?ttgcttccag?gagaaagttc?actgaaaaaa???2110
aaatatcttc?tggcttcttg?tgtgtacaga?gacaacagaa?ctcggtgggg?aaacgggaat???2170
cttttctgca?ccaaagctgc?ttctaaagca?gaaagcagtg?gggctcttgg?tgtttcatgc???2230
tgccttattt?atattaaagg?aagaattaaa?tcttaaaaaa?aaaaaaaaaa??????????????2280
<210>12
<211>148
<212>PRT
＜213〉homo sapiens (Homo sapiens)
<400>12
Met?Gly?Thr?Trp?Trp?His?Arg?Glu?Thr?Gln?Leu?Ala?Thr?Phe?Ser?Ala
1???????????????5???????????????????10??????????????????15
Thr?Leu?Leu?Thr?Gln?Leu?His?Pro?Thr?Leu?Leu?Thr?Gln?Val?His?Pro
20??????????????????25??????????????????30
Leu?Leu?Leu?Ser?Phe?Pro?Gln?Arg?Thr?Gly?Ser?Arg?Ala?Trp?Trp?Val
35??????????????????40??????????????????45
Phe?Ala?Thr?Leu?Gly?Ala?Ser?Ser?Ala?Ala?Pro?His?Leu?Ser?Leu?Phe
50??????????????????55??????????????????60
Ser?Pro?Lys?Leu?Val?Phe?Leu?Thr?Ile?Ile?Val?Val?Gly?Gly?Gly?Gln
65??????????????????70??????????????????75??????????????????80
Mer?Leu?Lys?Val?Glu?Ala?Asp?Leu?Glu?Lys?Glu?Thr?His?Gly?Val?Thr
85??????????????????90??????????????????95
Val?Ala?Lys?Asp?Ser?Trp?Lys?Arg?Asn?Ser?Ile?Thr?Ser?Ser?Leu?Ala
100?????????????????105?????????????????110
Thr?Thr?Arg?His?Pro?Arg?Pro?Trp?His?Ser?Gln?Arg?Leu?Cys?Ala?Glu
115?????????????????120?????????????????125
Pro?Ser?Leu?Ser?Thr?Ser?Ser?Gly?Pro?Ala?Ser?Cys?Ser?Glu?Val?Ser
130?????????????????135?????????????????140
Ala?Val?Arg?Gln
145
<210>13
<211>3750
<212>DNA
＜213〉homo sapiens (Homo sapiens)
<400>13
gtcgaagcgg?ctgcagagcc?ggtaacggtg?gtggcggctg?ttgggccaaa?ggcgaaagac?????60
gaagaggagg?aggaagagga?gccgctgcca?ccgtgcgagg?cggtgcgctg?ggccccagtg????120
ggggcggtgg?cggaggcccg?gcctggggca?accgcgtttt?tagaagaggc?gacggccgag????180
gagcctggcg?cggccccggg?ctccccgccg?gattcgccgg?gccggacgct?gcggcggctg????240
cgggcagagc?ggcggcggct?ggactcggcg?ctgctggcgc?tgtcctcgca?cttcgcgcag????300
gtgcagttcc?gcctgcgcca?ggtggtgcgc?ggggcgccgg?cggagcagca?gcgccttctg????360
cgggagctcg?aagacttcgc?cttccgcggc?tgccctcacg?tcctaggtta?cgaagggccc????420
ggcgaccccg?ccagcgatga?gggcgatggg?ctgccagggg?accggccacg?gttgcggggc????480
gaggaccagg?gcttgagtga?gcaggaaaag?caagagcgtc?tggaaaccca?aagggagaag????540
cagaaagaac?tgatactgca?gctcaagacc?cagctagatg?acctggaaac?gtttgcctat????600
caagagggca?gttatgactc?gctgccacag?tccgtggtgt?tggaaagaca?gcgggtgatc????660
atagatgagt?taataaagaa?actggacatg?aatctgaatg?aggacatcag?ttccctgtcc????720
actgaagagc?ttcgtcagcg?tgtagatgca?gcagtggctc?agatcgtcaa?cccagcccga????780
gtcaaagaac?agttggttga?gcaactgaaa?actcagatcc?gagaccttga?gatgtttatc????840
aacttcatcc?aagatgaagt?gggaagcccc?ttgcagacag?gtggtggaca?ctgtgagtgc????900
aaggccggtg?ggaagacagg?aaatggctgc?agcagaacag?gcagcagcag?aacgcctcca????960
ggaaacagca?aaacaaaggc?agaggatgtg?aagaaagtcc?gggagacggg?gctgcacctg???1020
atgcggcgag?cgctggccgt?gctccagatc?tttgctgtta?gccagtttgg?ttgtgccaca???1080
ggccagatcc?ctccaaccct?gtggcagagg?gtccaggctg?acagagacta?ctctcccttg???1140
ctgaagaggc?tggaggtgtc?agtggacaga?gtgaagcagc?tagccttgag?gcagcagcca???1200
catgaccatg?tcatcacctc?tgccaacctc?caggacctct?ctctgggagg?caaggatgag???1260
ctgactatgg?ctgtgcggaa?ggagctaacg?gtggctgtga?gggacctgct?ggcccatgga???1320
ctgtatgcct?cctccccagg?gatgagcctt?gttatggctc?ctattgcttg?tttgctgcca???1380
gccttctcct?cggccccaga?ggccatgcac?ccgtgggagc?tctttgtaaa?gtactaccat???1440
gctaagaacg?gccgtgctta?tgtggaatcc?ccagcccgga?agctctccca?gtccttcgcc???1500
cttcctgtta?cgggaggcac?tgttgtcacc?cccaaacaga?gcctactgac?agccatccac???1560
atggtgctga?cagagcatga?cccttttaag?cgcagtgcag?actcagaatt?gaaggccttg???1620
gtgtgcatgg?cactgaatga?gcagcgtctg?gtgtcctggg?tgaacctcat?ctgcaagtcc???1680
gggtcactca?tcgagcctca?ctaccagccc?tggagctaca?tggcacacac?aggctttgag???1740
agtgccctca?acctgctcag?tcgcctcagc?agcctcaagt?ttagcctccc?tgtagatctg???1800
gctgtgcgcc?agctcaaaaa?catcaaagat?gccttttgat?gagagtgccc?taaccccaga???1860
caagctcctt?gttcagtagg?gatagatgtg?ctagtcttct?agcataggag?caaggaatca???1920
gaggtggggt?taaaggcatt?tttcccagac?cctgctcagg?cagtcggcca?aatgagtgca???1980
aagtctgttt?tccccaccaa?cttagctctc?ggaaagatgt?gctggaggga?ccctcttgtt???2040
aagaaggttc?tgccaggccg?ggcgcggtgg?ctcacacctg?taatcccagc?actttgggag???2100
gccgaggcgg?gcggatcaca?aggtcaggag?ttcgagacca?tcctgtctaa?cacggtgaaa???2160
ccccatctct?actaaaaata?caaaaaaatt?agtcggacat?ggtggcaggc?acctgtagtc???2220
ccagctactc?gggaggctga?ggcaggagaa?tggcgtgaac?ccaggaggcg?gagcttgcag???2280
tgagccaaga?tcgcgccact?gcactccagc?ctgggcgaca?gagtgagact?ccgtctcaaa???2340
aaaaaaaaag?aaggttgtac?ccacagtata?cgtgtgggca?cttgtgcttg?agcctgtatt???2400
aaaggaatca?ggccaggcac?agtggctcac?gcctgtaatc?tcagcacatt?gggaggctga???2460
ggtgggcgga?tcacctgagg?tcaggagttc?aagaccagcc?tggccaacat?ggtgaaaccc???2520
catctctact?aaaaatacaa?aaattagcta?ggcggggtag?tgcatgcctg?taatcccagc???2580
tactcaggag?gctgaggccg?gagaatcgct?tgagcccgag?aggtggagac?tgcagtaagc???2640
caagatcatg?ccactgcact?ccagcctggg?caacacaggg?agactccatc?tcaaaaaaaa???2700
aaaaaaaaaa?aaaaagacat?tcaacttgag?gctcctgtta?gttaagctat?cttctttcac???2760
ttgaagcagg?tttgagaggc?ctaggccaga?atttaaattc?cttttatgaa?tagatttccc???2820
tttcttcctg?accccaaggt?cagaggagac?tatatattcc?atggctgcct?ctaagactag???2880
gaataggaat?atctgaaaac?agcatttcta?agggtggtaa?ccacaggtcg?attttaatac???2940
gagtcctttt?tcttgtagag?gtaagtaaaa?tcttcctgac?aaggtagtcc?tcttttcacg???3000
gcacagacaa?tgggctttct?gtttatgagg?ggtgagaagt?gatgtttgtt?actatgttct???3060
ccagcaagta?aacattcctc?tgctcacctc?ccaacaagac?taacagtctt?tttagaagta???3120
aatatattca?agacaaacga?gaaaatcctg?gctacccaag?tcgagtatat?acaggaatac???3180
aaatcggtaa?ccagcagctg?ttcctcaggt?tgtgactcac?tgagcaccac?ttgtcctgga???3240
ggctggatga?tggaggacat?ctggttatag?gtactgtagc?agggataggt?gccacaggag???3300
gataggaact?acagcagatc?gctgtttcca?gaacggggag?gagtatctca?ttgtgaaaca???3360
gactctagag?tggttctatt?tggtcttcag?tgttttagcc?tcattagttc?atatttggca???3420
tgcagcttgt?ggtgagtact?gttctaggac?tggccaaaaa?tgggcaaaat?gtatcactcc???3480
aaacactact?gattcagcat?tgttttcatg?tcttaaaatt?gccacctgca?ctttgtttct???3540
gcactattat?gtagtgcatt?ttaacttaaa?ttttttccag?caacatgtta?cttatttaag???3600
atacattact?gatatttcat?tataattagt?tcaccttccc?tgtgaaacaa?gagaattgta???3660
aaatgttgtg?gaaaatgata?catatgtgga?tgctaatgaa?atcatagtat?tttgtgtagc???3720
ttctctgaaa?aaaaaaaaaa?aaaaaaaaaa????????????????????????????????????3750
<210>14
<211>3750
<212>DNA
＜213〉homo sapiens (Homo sapiens)
<220>
<221>CDS
<222>(688)..(1836)
<223>
<400>14
gtcgaagcgg?ctgcagagcc?ggtaacggtg?gtggcggctg?ttgggccaaa?ggcgaaagac?????60
gaagaggagg?aggaagagga?gccgctgcca?ccgtgcgagg?cggtgcgctg?ggccccagtg????120
ggggcggtgg?cggaggcccg?gcctggggca?accgcgtttt?tagaagaggc?gacggccgag????180
gagcctggcg?cggccccggg?ctccccgccg?gattcgccgg?gccggacgct?gcggcggctg????240
cgggcagagc?ggcggcggct?ggactcggcg?ctgctggcgc?tgtcctcgca?cttcgcgcag????300
gtgcagttcc?gcctgcgcca?ggtggtgcgc?ggggcgccgg?cggagcagca?gcgccttctg????360
cgggagctcg?aagacttcgc?cttccgcggc?tgccctcacg?tcctaggtta?cgaagggccc????420
ggcgaccccg?ccagcgatga?gggcgatggg?ctgccagggg?accggccacg?gttgcggggc????480
gaggaccagg?gcttgagtga?gcaggaaaag?caagagcgtc?tggaaaccca?aagggagaag????540
cagaaagaac?tgatactgca?gctcaagacc?cagctagatg?acctggaaac?gtttgcctat????600
caagagggca?gttatgactc?gctgccacag?tccgtggtgt?tggaaagaca?gcgggtgatc????660
atagatgagt?taataaagaa?actggac?atg?aat?ctg?aat?gag?gac?atc?agt?tcc????714
Met?Asn?Leu?Asn?Glu?Asp?Ile?Ser?Ser
1???????????????5
ctg?tcc?act?gaa?gag?ctt?cgt?cag?cgt?gta?gat?gca?gca?gtg?gct?cag??????762
Leu?Ser?Thr?Glu?Glu?Leu?Arg?Gln?Arg?Val?Asp?Ala?Ala?Val?Ala?Gln
10??????????????????15??????????????????20??????????????????25
atc?gtc?aac?cca?gcc?cga?gtc?aaa?gaa?cag?ttg?gtt?gag?caa?ctg?aaa??????810
Ile?Val?Asn?Pro?Ala?Arg?Val?Lys?Glu?Gln?Leu?Val?Glu?Gln?Leu?Lys
30??????????????????35??????????????????40
act?cag?atc?cga?gac?ctt?gag?atg?ttt?atc?aac?ttc?atc?caa?gat?gaa??????858
Thr?Gln?Ile?Arg?Asp?Leu?Glu?Met?Phe?Ile?Asn?Phe?Ile?Gln?Asp?Glu
45??????????????????50??????????????????55
gtg?gga?agc?ccc?ttg?cag?aca?ggt?ggt?gga?cac?tgt?gag?tgc?aag?gcc??????906
Val?Gly?Ser?Pro?Leu?Gln?Thr?Gly?Gly?Gly?His?Cys?Glu?Cys?Lys?Ala
60??????????????????65??????????????????70
ggt?ggg?aag?aca?gga?aat?ggc?tgc?agc?aga?aca?ggc?agc?agc?aga?acg??????954
Gly?Gly?Lys?Thr?Gly?Asn?Gly?Cys?Ser?Arg?Thr?Gly?Ser?Ser?Arg?Thr
75??????????????????80??????????????????85
cct?cca?gga?aac?agc?aaa?aca?aag?gca?gag?gat?gtg?aag?aaa?gtc?cgg?????1002
Pro?Pro?Gly?Asn?Ser?Lys?Thr?Lys?Ala?Glu?Asp?Val?Lys?Lys?Vat?Arg
90??????????????????95??????????????????100?????????????????105
gag?acg?ggg?ctg?cac?ctg?atg?cgg?cga?gcg?ctg?gcc?gtg?ctc?cag?atc?????1050
Glu?Thr?Gly?Leu?His?Leu?Met?Arg?Arg?Ala?Leu?Ala?Val?Leu?Gln?Ile
110?????????????????115?????????????????120
ttt?gct?gtt?agc?cag?ttt?ggt?tgt?gcc?aca?ggc?cag?atc?cct?cca?acc?????1098
Phe?Ala?Val?Ser?Gln?Phe?Gly?Cys?Ala?Thr?Gly?Gln?Ile?Pro?Pro?Thr
125?????????????????130?????????????????135
ctg?tgg?cag?agg?gtc?cag?gct?gac?aga?gac?tac?tct?ccc?ttg?ctg?aag?????1146
Leu?Trp?Gln?Arg?Val?Gln?Ala?Asp?Arg?Asp?Tyr?Ser?Pro?Leu?Leu?Lys
140?????????????????145?????????????????150
agg?ctg?gag?gtg?tca?gtg?gac?aga?gtg?aag?cag?cta?gcc?ttg?agg?cag?????1194
Arg?Leu?Glu?Val?Ser?Val?Asp?Arg?Val?Lys?Gln?Leu?Ala?Leu?Arg?Gln
155?????????????????160?????????????????165
cag?cca?cat?gac?cat?gtc?atc?acc?tct?gcc?aac?ctc?cag?gac?ctc?tct?????1242
Gln?Pro?His?Asp?His?Val?Ile?Thr?Ser?Ala?Asn?Leu?Gln?Asp?Leu?Ser
170?????????????????175?????????????????180?????????????????185
ctg?gga?ggc?aag?gat?gag?ctg?act?atg?gct?gtg?cgg?aag?gag?cta?acg?????1290
Leu?Gly?Gly?Lys?Asp?Glu?Leu?Thr?Met?Ala?Val?Arg?Lys?Glu?Leu?Thr
190?????????????????195?????????????????200
gtg?gct?gtg?agg?gac?ctg?ctg?gcc?cat?gga?ctg?tat?gcc?tcc?tcc?cca?????1338
Val?Ala?Val?Arg?Asp?Leu?Leu?Ala?His?Gly?Leu?Tyr?Ala?Ser?Ser?Pro
205?????????????????210?????????????????215
ggg?atg?agc?ctt?gtt?atg?gct?cct?att?gct?tgt?ttg?ctg?cca?gcc?ttc?????1386
Gly?Met?Ser?Leu?Val?Met?Ala?Pro?Ile?Ala?Cys?Leu?Leu?Pro?Ala?Phe
220?????????????????225?????????????????230
tcc?tcg?gcc?cca?gag?gcc?atg?cac?ccg?tgg?gag?ctc?ttt?gta?aag?tac?????1434
Ser?Ser?Ala?Pro?Glu?Ala?Met?His?Pro?Trp?Glu?Leu?Phe?Val?Lys?Tyr
235?????????????????240?????????????????245
tac?cat?gct?aag?aac?ggc?cgt?gct?tat?gtg?gaa?tcc?cca?gcc?cgg?aag?????1482
Tyr?His?Ala?Lys?Asn?Gly?Arg?Ala?Tyr?Val?Glu?Ser?Pro?Ala?Arg?Lys
250?????????????????255?????????????????260?????????????????265
ctc?tcc?cag?tcc?ttc?gcc?ctt?cct?gtt?acg?gga?ggc?act?gtt?gtc?acc?????1530
Leu?Ser?Gln?Ser?Phe?Ala?Leu?Pro?Val?Thr?Gly?Gly?Thr?Val?Val?Thr
270?????????????????275?????????????????280
ccc?aaa?cag?agc?cta?ctg?aca?gcc?atc?cac?atg?gtg?ctg?aca?gag?cat?????1578
Pro?Lys?Gln?Ser?Leu?Leu?Thr?Ala?Ile?His?Met?Val?Leu?Thr?Glu?His
285?????????????????290?????????????????295
gac?cct?ttt?aag?cgc?agt?gca?gac?tca?gaa?ttg?aag?gcc?ttg?gtg?tgc?????1626
Asp?Pro?Phe?Lys?Arg?Ser?Ala?Asp?Set?Glu?Leu?Lys?Ala?Leu?Val?Cys
300?????????????????305?????????????????310
atg?gca?ctg?aat?gag?cag?cgt?ctg?gtg?tcc?tgg?gtg?aac?ctc?atc?tgc?????1674
Met?Ala?Leu?Asn?Glu?Gln?Arg?Leu?Val?Ser?Trp?Val?Asn?Leu?Ile?Cys
315?????????????????320?????????????????325
aag?tcc?ggg?tca?ctc?atc?gag?cct?cac?tac?cag?ccc?tgg?agc?tac?atg?????1722
Lys?Ser?Gly?Set?Leu?Ile?Glu?Pro?His?Tyr?Gln?Pro?Trp?Ser?Tyr?Met
330?????????????????335?????????????????340?????????????????345
gca?cac?aca?ggc?ttt?gag?agt?gcc?ctc?aac?ctg?ctc?agt?cgc?ctc?agc?????1770
Ala?His?Thr?Gly?Phe?Glu?Ser?Ala?Leu?Asn?Leu?Leu?Ser?Arg?Leu?Ser
350?????????????????355?????????????????360
agc?ctc?aag?ttt?agc?ctc?cct?gta?gat?ctg?gct?gtg?cgc?cag?ctc?aaa?????1818
Ser?Leu?Lys?Phe?Ser?Leu?Pro?Val?Asp?Leu?Ala?Val?Arg?Gln?Leu?Lys
365?????????????????370?????????????????375
aac?atc?aaa?gat?gcc?ttt?tgatgagagt?gccctaaccc?cagacaagct????????????1866
Asn?Ile?Lys?Asp?Ala?Phe
380
ccttgttcag?tagggataga?tgtgctagtc?ttctagcata?ggagcaagga?atcagaggtg???1926
gggttaaagg?catttttccc?agaccctgct?caggcagtcg?gccaaatgag?tgcaaagtct???1986
gttttcccca?ccaacttagc?tctcggaaag?atgtgctgga?gggaccctct?tgttaagaag???2046
gttctgccag?gccgggcgcg?gtggctcaca?cctgtaatcc?cagcactttg?ggaggccgag???2106
gcgggcggat?cacaaggtca?ggagttcgag?accatcctgt?ctaacacggt?gaaaccccat???2166
ctctactaaa?aatacaaaaa?aattagtcgg?acatggtggc?aggcacctgt?agtcccagct???2226
actcgggagg?ctgaggcagg?agaatggcgt?gaacccagga?ggcggagctt?gcagtgagcc???2286
aagatcgcgc?cactgcactc?cagcctgggc?gacagagtga?gactccgtct?caaaaaaaaa???2346
aaagaaggtt?gtacccacag?tatacgtgtg?ggcacttgtg?cttgagcctg?tattaaagga???2406
atcaggccag?gcacagtggc?tcacgcctgt?aatctcagca?cattgggagg?ctgaggtggg???2466
cggatcacct?gaggtcagga?gttcaagacc?agcctggcca?acatggtgaa?accccatctc???2526
tactaaaaat?acaaaaatta?gctaggcggg?gtagtgcatg?cctgtaatcc?cagctactca???2586
ggaggctgag?gccggagaat?cgcttgagcc?cgagaggtgg?agactgcagt?aagccaagat???2646
catgccactg?cactccagcc?tgggcaacac?agggagactc?catctcaaaa?aaaaaaaaaa???2706
aaaaaaaaag?acattcaact?tgaggctcct?gttagttaag?ctatcttctt?tcacttgaag???2766
caggtttgag?aggcctaggc?cagaatttaa?attcctttta?tgaatagatt?tccctttctt???2826
cctgacccca?aggtcagagg?agactatata?ttccatggct?gcctctaaga?ctaggaatag???2886
gaatatctga?aaacagcatt?tctaagggtg?gtaaccacag?gtcgatttta?atacgagtcc???2946
tttttcttgt?agaggtaagt?aaaatcttcc?tgacaaggta?gtcctctttt?cacggcacag???3006
acaatgggct?ttctgtttat?gaggggtgag?aagtgatgtt?tgttactatg?ttctccagca???3066
agtaaacatt?cctctgctca?cctcccaaca?agactaacag?tctttttaga?agtaaatata???3126
ttcaagacaa?acgagaaaat?cctggctacc?caagtcgagt?atatacagga?atacaaatcg???3186
gtaaccagca?gctgttcctc?aggttgtgac?tcactgagca?ccacttgtcc?tggaggctgg???3246
atgatggagg?acatctggtt?ataggtactg?tagcagggat?aggtgccaca?ggaggatagg???3306
aactacagca?gatcgctgtt?tccagaacgg?ggaggagtat?ctcattgtga?aacagactct???3366
agagtggttc?tatttggtct?tcagtgtttt?agcctcatta?gttcatattt?ggcatgcagc???3426
ttgtggtgag?tactgttcta?ggactggcca?aaaatgggca?aaatgtatca?ctccaaacac???3486
tactgattca?gcattgtttt?catgtcttaa?aattgccacc?tgcactttgt?ttctgcacta???3546
ttatgtagtg?cattttaact?taaatttttt?ccagcaacat?gttacttatt?taagatacat???3606
tactgatatt?tcattataat?tagttcacct?tccctgtgaa?acaagagaat?tgtaaaatgt???3666
tgtggaaaat?gatacatatg?tggatgctaa?tgaaatcata?gtattttgtg?tagcttctct???3726
gaaaaaaaaa?aaaaaaaaaa?aaaa??????????????????????????????????????????3750
<210>15
<211>383
<212>PRT
＜213〉homo sapiens (Homo sapiens)
<400>15
Met?Asn?Leu?Asn?Glu?Asp?Ile?Ser?Ser?Leu?Ser?Thr?Glu?Glu?Leu?Arg
1???????????????5???????????????????10??????????????????15
Gln?Arg?Val?Asp?Ala?Ala?Val?Ala?Gln?Ile?Val?Asn?Pro?Ala?Arg?Val
20??????????????????25??????????????????30
Lys?Glu?Gln?Leu?Val?Glu?Gln?Leu?Lys?Thr?Gln?Ile?Arg?Asp?Leu?Glu
35??????????????????40??????????????????45
Met?Phe?Ile?Asn?Phe?Ile?Gln?Asp?Glu?Val?Gly?Ser?Pro?Leu?Gln?Thr
50??????????????????55??????????????????60
Gly?Gly?Gly?His?Cys?Glu?Cys?Lys?Ala?Gly?Gly?Lys?Thr?Gly?Asn?Gly
65??????????????????70??????????????????75??????????????????80
Cys?Ser?Arg?Thr?Gly?Ser?Ser?Arg?Thr?Pro?Pro?Gly?Asn?Ser?Lys?Thr
85??????????????????90??????????????????95
Lys?Ala?Glu?Asp?Val?Lys?Lys?Val?Arg?Glu?Thr?Gly?Leu?His?Leu?Met
100?????????????????105?????????????????110
Arg?Arg?Ala?Leu?Ala?Val?Leu?Gln?Ile?Phe?Ala?Val?Ser?Gln?Phe?Gly
115?????????????????120?????????????????125
Cys?Ala?Thr?Gly?Gln?Ile?Pro?Pro?Thr?Leu?Trp?Gln?Arg?Val?Gln?Ala
130?????????????????135?????????????????140
Asp?Arg?Asp?Tyr?Ser?Pro?Leu?Leu?Lys?Arg?Leu?Glu?Val?Ser?Val?Asp
145?????????????????150?????????????????155?????????????????160
Arg?Val?Lys?Gln?Leu?Ala?Leu?Arg?Gln?Gln?Pro?His?Asp?His?Val?Ile
165?????????????????170?????????????????175
Thr?Ser?Ala?Asn?Leu?Gln?Asp?Leu?Ser?Leu?Gly?Gly?Lys?Asp?Glu?Leu
180?????????????????185?????????????????190
Thr?Met?Ala?Val?Arg?Lys?Glu?Leu?Thr?Val?Ala?Val?Arg?Asp?Leu?Leu
195?????????????????200?????????????????205
Ala?His?Gly?Leu?Tyr?Ala?Ser?Ser?Pro?Gly?Met?Ser?Leu?Val?Met?Ala
210?????????????????215?????????????????220
Pro?Ile?Ala?Cys?Leu?Leu?Pro?Ala?Phe?Ser?Ser?Ala?Pro?Glu?Ala?Met
225?????????????????230?????????????????235?????????????????240
His?Pro?Trp?Glu?Leu?Phe?Val?Lys?Tyr?Tyr?His?Ala?Lys?Asn?Gly?Arg
245?????????????????250?????????????????255
Ala?Tyr?Val?Glu?Ser?Pro?Ala?Arg?Lys?Leu?Ser?Gln?Ser?Phe?Ala?Leu
260?????????????????265?????????????????270
Pro?Val?Thr?Gly?Gly?Thr?Val?Val?Thr?Pro?Lys?Gln?Ser?Leu?Leu?Thr
275?????????????????280?????????????????285
Ala?Ile?His?Met?Val?Leu?Thr?Glu?His?Asp?Pro?Phe?Lys?Arg?Ser?Ala
290?????????????????295?????????????????300
Asp?Ser?Glu?Leu?Lys?Ala?Leu?Val?Cys?Met?Ala?Leu?Asn?Glu?Gln?Arg
305?????????????????310?????????????????315?????????????????320
Leu?Val?Ser?Trp?Val?Asn?Leu?Ile?Cys?Lys?Ser?Gly?Ser?Leu?Ile?Glu
325?????????????????330?????????????????335
Pro?His?Tyr?Gln?Pro?Trp?Ser?Tyr?Met?Ala?His?Thr?Gly?Phe?Glu?Ser
340?????????????????345?????????????????350
Ala?Leu?Asn?Leu?Leu?Ser?Arg?Leu?Ser?Ser?Leu?Lys?Phe?Ser?Leu?Pro
355?????????????????360?????????????????365
Val?Asp?Leu?Ala?Val?Arg?Gln?Leu?Lys?Asn?Ile?Lys?Asp?Ala?Phe
370?????????????????375?????????????????380
<210>16
<211>2730
<212>DNA
＜213〉homo sapiens (Homo sapiens)
<400>16
gcgctcctcc?ggtggccgaa?cgtcgctttc?gggagctgtg?gtccccgcaa?gccgcggttc?????60
ccggcctttc?agctcggctg?ctccctgggt?ggtggggccg?aggcgcaccc?ccttctgccc????120
gggcccggag?tgcggaggcg?cagcccggga?gaggggctga?aggggggcag?gcagtcctcg????180
ggcgcgagtg?tgcagcgccc?tctgggcgtg?gacgcggagc?gcgctccagg?tgcgcaggac????240
tcctagctct?cttggggaac?gctttctggg?gcagggcggg?gacccggggg?cgatggggcc????300
ttcaaccgac?gggcaaacac?acacagacac?accccggctg?tcctccagct?gcgatgcggc????360
tcgctgcgcc?ccgccggccc?agattctccc?gtacgctcgc?gttcttcctg?cactccacaa????420
agtgctggag?acttggggcc?ttagtttaaa?cagctgcacc?cgcacggaag?ggctgcaaga????480
aaaaggggga?cgggggcgga?gttcgcagcg?tctttctgtg?gaagacaacg?ccctctctct????540
tcatctgagc?taaaaggaaa?aataagtaac?ttctttgttc?cccggtgacc?tcgggcacgc????600
ggatctgagg?agcaccgtgc?agttgctgct?ccttcgagat?cccctgcgag?gccctgggcc????660
tcagttcaaa?aagctgtgga?aggcctgcgg?gtgtagggga?aacggccgag?agagcaaagg????720
ggaagaaccg?gacgggccct?gcccacatct?ggcaggcagg?agagatggtg?ccaaacgacc????780
ttcgcacctg?ggccctgaga?ccaaagatgg?cgtcgtcctc?ccctgtcttc?cctaggcgga????840
aggcaagaac?ttgggtcggc?ctgagctggg?gaccaagaaa?atatcgggaa?gtgtccagtg????900
ttcctcttta?aaagaattaa?accttcctgg?gccacgcagg?gactgaccgt?gggaccctgg????960
actctcctcc?accttccacc?gtggagcagg?ctctggagcc?gagcagcctc?ctgcgtcccc???1020
atctcatccc?cccactcctc?ctcacagcac?taaaagatga?tggcctgctt?gtacccctgc???1080
tcctgagcac?ctgtttgacc?tcaaagagct?ctcgcttttg?gggtgtccgt?ctccccaagt???1140
ctgtctaggg?ggcaggaaga?ccttcatcag?ggttcttggg?ggccgatgtg?ggctcgctga???1200
gtgcctgagt?gatggatggt?ctcacagcac?ggcaccacgg?aggtccagcc?gagctcgagg???1260
agtccctgtg?gattaaaaat?gcccttttcg?aaggttctac?ctctaccaga?gggtggctcc???1320
acagatccct?tcaacccagt?atggtggaaa?aataggaatt?ttggaatctt?acctttctct???1380
actgctcaca?acagaggaaa?ctggggtcag?ggaggatttg?atggagtctt?ttcatttttt???1440
aaaagtcact?cacggccaag?cgcggtggct?cacgcctgtg?accccagcac?tttgggaggc???1500
cgaggcgggc?agatcacctg?aggtcaggag?ttctcgacca?gcctggctaa?cacggtgaat???1560
ccccatctct?actaaaaata?caaaaattag?ccggatatgg?tggcaggtgc?tataatccca???1620
gctactcggg?aggctgaggc?agggagaatc?gcctcgacct?ctcgggttca?aacaattctc???1680
ccgcctcagc?ctcctaaata?gctgggatta?cagtcgcacg?ccaccacagg?ccggctaatt???1740
ttttatttat?ttatttattt?tgatttttag?tagagacaag?gtttcaccat?attggtcagg???1800
ctggtcttga?actcccaacc?tcaggtgata?cacccgtctc?ggcctcccaa?agcgctggga???1860
ttgcaggcgt?gagccaccgt?gcctggccct?tatacttgtc?ttcttgagtg?catgtgtgag???1920
gctttctcta?gaagtagagc?tctggattat?aggtataaac?atcttcaact?ttctcaggaa???1980
ttgttgtaag?caattgctct?aagcaaattg?ctctccaaag?ggcttatagt?aatttacact???2040
cccaacagca?gttatgagca?tttccatttt?tctacatcct?cctatgactt?ggtgttgggc???2100
cttttatatt?ttgccagctg?atgcaagtaa?aattatatct?tattgctgtt?tgagtctgac???2160
catccttccc?attaggaaat?gttttgctga?agcctttagt?tgccgactgg?ttgggcagac???2220
acttctgttt?acttatcaca?tctgttttcc?tctttctcag?taagagattc?cttaaatttc???2280
aattgggctt?atggctattt?aaaataaaga?ttacctttcc?ctgcctctct?tacagccacg???2340
cgtagccaca?tgattagggt?cttgccagtg?gcacatgagc?agaagtggta?catgcacttt???2400
cagaaaggtg?tcaagcatgc?tctccttccc?ttttctgttt?tctcctgggc?ggaatacaga???2460
tctgatggct?gcctctgaag?cagccttctt?ggaccatgaa?gtgaacatgg?gaacagaggc???2520
catccacagc?agaggaacaa?gttaaaggaa?gcatgggtcc?ctgccccatg?gagtatagcg???2580
tcatccctgg?actgcccacg?cagacttata?catgatggga?aaatgtctct?ttttgtttgt???2640
ttaggacact?gtttctctga?attttctctc?acttgccagc?aattctaatc?ctaaaaatac???2700
actgatgaat?tcaaaaaaaa?aaaaaaaaaa????????????????????????????????????2730
<210>17
<211>2730
<212>DNA
＜213〉homo sapiens (Homo sapiens)
<220>
<221>CDS
<222>(1216)..(1518)
<223>
<400>17
gcgctcctcc?ggtggccgaa?cgtcgctttc?gggagctgtg?gtccccgcaa?gccgcggttc?????60
ccggcctttc?agctcggctg?ctccctgggt?ggtggggccg?aggcgcaccc?ccttctgccc????120
gggcccggag?tgcggaggcg?cagcccggga?gaggggctga?aggggggcag?gcagtcctcg????180
ggcgcgagtg?tgcagcgccc?tctgggcgtg?gacgcggagc?gcgctccagg?tgcgcaggac????240
tcctagctct?cttggggaac?gctttctggg?gcagggcggg?gacccggggg?cgatggggcc????300
ttcaaccgac?gggcaaacac?acacagacac?accccggctg?tcctccagct?gcgatgcggc????360
tcgctgcgcc?ccgccggccc?agattctccc?gtacgctcgc?gttcttcctg?cactccacaa????420
agtgctggag?acttggggcc?ttagtttaaa?cagctgcacc?cgcacggaag?ggctgcaaga????480
aaaaggggga?cgggggcgga?gttcgcagcg?tctttctgtg?gaagacaacg?ccctctctct????540
tcatctgagc?taaaaggaaa?aataagtaac?ttctttgttc?cccggtgacc?tcgggcacgc????600
ggatctgagg?agcaccgtgc?agttgctgct?ccttcgagat?cccctgcgag?gccctgggcc????660
tcagttcaaa?aagctgtgga?aggcctgcgg?gtgtagggga?aacggccgag?agagcaaagg????720
ggaagaaccg?gacgggccct?gcccacatct?ggcaggcagg?agagatggtg?ccaaacgacc????780
ttcgcacctg?ggccctgaga?ccaaagatgg?cgtcgtcctc?ccctgtcttc?cctaggcgga????840
aggcaagaac?ttgggtcggc?ctgagctggg?gaccaagaaa?atatcgggaa?gtgtccagtg????900
ttcctcttta?aaagaattaa?accttcctgg?gccacgcagg?gactgaccgt?gggaccctgg????960
actctcctcc?accttccacc?gtggagcagg?ctctggagcc?gagcagcctc?ctgcgtcccc???1020
atctcatccc?cccactcctc?ctcacagcac?taaaagatga?tggcctgctt?gtacccctgc???1080
tcctgagcac?ctgtttgacc?tcaaagagct?ctcgcttttg?gggtgtccgt?ctccccaagt???1140
ctgtctaggg?ggcaggaaga?ccttcatcag?ggttcttggg?ggccgatgtg?ggctcgctga???1200
gtgcctgagt?gatgg?atg?gtc?tca?cag?cac?ggc?acc?acg?gag?gtc?cag?ccg????1251
Met?Val?Ser?Gln?His?GIy?Thr?Thr?Glu?Val?Gln?Pro
1???????????????5???????????????????10
agc?tcg?agg?agt?ccc?tgt?gga?tta?aaa?atg?ccc?ttt?tcg?aag?gtt?cta?????1299
Ser?Ser?Arg?Ser?Pro?Cys?Gly?Leu?Lys?Met?Pro?Phe?Ser?Lys?Val?Leu
15??????????????????20??????????????????25
cct?cta?cca?gag?ggt?ggc?tcc?aca?gat?ccc?ttc?aac?cca?gta?tgg?tgg?????1347
Pro?Leu?Pro?Glu?Gly?Gly?Ser?Thr?Asp?Pro?Phe?Asn?Pro?Val?Trp?Trp
30??????????????????35??????????????????40
aaa?aat?agg?aat?ttt?gga?atc?tta?cct?ttc?tct?act?gct?cac?aac?aga?????1395
Lys?Asn?Arg?Asn?Phe?Gly?Ile?Leu?Pro?Phe?Ser?Thr?Ala?His?Asn?Arg
45??????????????????50??????????????????55??????????????????60
gga?aac?tgg?ggt?cag?gga?gga?ttt?gat?gga?gtc?ttt?tca?ttt?ttt?aaa?????1443
Gly?Asn?Trp?Gly?Gln?Gly?Gly?Phe?Asp?Gly?Val?Phe?Ser?Phe?Phe?Lys
65??????????????????70??????????????????75
agt?cac?tca?cgg?cca?agc?gcg?gtg?gct?cac?gcc?tgt?gac?ccc?agc?act?????1491
Ser?His?Ser?Arg?Pro?Ser?Ala?Val?Ala?His?Ala?Cys?Asp?Pro?Ser?Thr
80??????????????????85??????????????????90
ttg?gga?ggc?cga?ggc?ggg?cag?atc?acc?tgaggtcagg?agttctcgac???????????1538
Leu?Gly?Gly?Arg?Gly?Gly?Gln?Ile?Thr
95??????????????????100
cagcctggct?aacacggtga?atccccatct?ctactaaaaa?tacaaaaatt?agccggatat???1598
ggtggcaggt?gctataatcc?cagctactcg?ggaggctgag?gcagggagaa?tcgcctcgac???1658
ctctcgggtt?caaacaattc?tcccgcctca?gcctcctaaa?tagctgggat?tacagtcgca???1718
cgccaccaca?ggccggctaa?ttttttattt?atttatttat?tttgattttt?agtagagaca???1778
aggtttcacc?atattggtca?ggctggtctt?gaactcccaa?cctcaggtga?tacacccgtc???1838
tcggcctccc?aaagcgctgg?gattgcaggc?gtgagccacc?gtgcctggcc?cttatacttg???1898
tcttcttgag?tgcatgtgtg?aggctttctc?tagaagtaga?gctctggatt?ataggtataa???1958
acatcttcaa?ctttctcagg?aattgttgta?agcaattgct?ctaagcaaat?tgctctccaa???2018
agggcttata?gtaatttaca?ctcccaacag?cagttatgag?catttccatt?tttctacatc???2078
ctcctatgac?ttggtgttgg?gccttttata?ttttgccagc?tgatgcaagt?aaaattatat???2138
cttattgctg?tttgagtctg?accatccttc?ccattaggaa?atgttttgct?gaagccttta???2198
gttgccgact?ggttgggcag?acacttctgt?ttacttatca?catctgtttt?cctctttctc???2258
agtaagagat?tccttaaatt?tcaattgggc?ttatggctat?ttaaaataaa?gattaccttt???2318
ccctgcctct?cttacagcca?cgcgtagcca?catgattagg?gtcttgccag?tggcacatga???2378
gcagaagtgg?tacatgcact?ttcagaaagg?tgtcaagcat?gctctccttc?ccttttctgt???2438
tttctcctgg?gcggaataca?gatctgatgg?ctgcctctga?agcagccttc?ttggaccatg???2498
aagtgaacat?gggaacagag?gccatccaca?gcagaggaac?aagttaaagg?aagcatgggt???2558
ccctgcccca?tggagtatag?cgtcatccct?ggactgccca?cgcagactta?tacatgatgg???2618
gaaaatgtct?ctttttgttt?gtttaggaca?ctgtttctct?gaattttctc?tcacttgcca???2678
gcaattctaa?tcctaaaaat?acactgatga?attcaaaaaa?aaaaaaaaaa?aa???????????2730
<210>18
<211>101
<212>PRT
＜213〉homo sapiens (Homo sapiens)
<400>18
Met?Val?Ser?Gln?His?Gly?Thr?Thr?Glu?Val?Gln?Pro?Ser?Ser?Arg?Ser
1???????????????5???????????????????10??????????????????15
Pro?Cys?Gly?Leu?Lys?Met?Pro?Phe?Ser?Lys?Val?Leu?Pro?Leu?Pro?Glu
20??????????????????25??????????????????30
Gly?Gly?Ser?Thr?Asp?Pro?Phe?Asn?Pro?Val?Trp?Trp?Lys?Asn?Arg?Asn
35??????????????????40??????????????????45
Phe?Gly?Ile?Leu?Pro?Phe?Ser?Thr?Ala?His?Asn?Arg?Gly?Asn?Trp?Gly
50??????????????????55??????????????????60
Gln?Gly?Gly?Phe?Asp?Gly?Val?Phe?Ser?Phe?Phe?Lys?Ser?His?Ser?Arg
65??????????????????70??????????????????75??????????????????80
Pro?Ser?Ala?Val?Ala?His?Ala?Cys?Asp?Pro?Ser?Thr?Leu?Gly?Gly?Arg
85??????????????????90??????????????????95
Gly?Gly?Gln?Ile?Thr
100
<210>19
<211>3260
<212>DNA
＜213〉homo sapiens (Homo sapiens)
<400>19
gggggatccg?tgctgccatg?gagcgtgccg?gcaagcagga?gatgctgctg?aagccacata?????60
gccgtgtcca?ggtattcgag?ggtgcggagg?acaacctccc?ggaccgcgat?gcgctgcggg????120
ctgcactggc?catccagcaa?ctggctgagg?gactcacagc?tgatgacctg?ctgctcgtgc????180
tgatctcagg?tgtggtacca?cattggccca?agactgttgg?tggggggtgc?accacctgat????240
ctctcaggtc?ttcgagagat?caggtctgag?cccccggggt?cacagggtaa?agttaggagc????300
aagggaggtg?gtgaggagcc?tggatggtga?ctcctgggtt?gccttgggct?agtcgtctgg????360
cctcccctga?gctgtggagt?cctccccctg?agctgacctt?ttcggtgaca?gtgagagggt????420
gcatggggag?cacataatgc?agggcctgac?acatccgtag?agtccttgga?aaatgtcact????480
gctattgctg?ctgtctgtac?taatgtcacc?tggggctagg?ccctcagacc?tcatggagtc????540
ctctccttga?tcttccattg?tgtgccccct?gccctgcctc?ctgttctagt?ctcttaagga????600
gtagttagtt?cttccggtgc?ctactgggac?ctggggtacc?ccaaggcagg?ggcaacctca????660
ggtggccagg?atgtggtgac?tgtacacata?ttgcatatcc?acaggggggc?accctaaccc????720
cacctttcca?gccccctttt?tctaatgtgg?gcttgtcacc?cctgcttgcc?tggttccctg????780
ggttgggata?gcccctctac?ctgataccct?cattgtcttg?agaggtgggg?gttcagctct????840
gctgcctgcc?cccatcccac?ctgtcacact?ggaggagaag?cagacactca?ctagactgct????900
ggcagcccgt?ggagccacca?tccaggagtt?gaacaccatt?cggaaggccc?tgtcccagct????960
caagggtggg?gggctggctc?aggccgccta?ccctgcccag?gtggtgagcc?tcatcctgtc???1020
agatgtggtg?gggggggggc?accctgtgga?ggtgattgcc?agtggcccca?ccgtggccag???1080
ttcccacaat?gtgcaagatt?gcctgcatat?cctcaatcgc?tacggcctcc?gtgcagccct???1140
gccacgttct?gtgaagactg?tgctgtctcg?ggccgactct?gacccccatg?ggccacacac???1200
ctgtggccat?gtcctgaatg?tgatcattgg?ctctaatgtg?ctggcgctag?ctgaggccca???1260
gcggcaggcc?gaggcactgg?gctaccaggc?tgtgttgctg?agtgcagcca?tgcaaggtga???1320
tgtaaaaagt?atggcccagt?tctacgggct?gctggcccat?gtggctagaa?cccgcctcac???1380
cccatccatg?gctggggctt?ctgtggagga?agatgcacag?ctccatgagc?tggcagctga???1440
gcttcagatc?ccagacctgc?agctggagga?ggctctggag?accatggcat?ggggaagggg???1500
cccagtctgc?ctgctggctg?gtggcgagcc?cacagtacag?ctgcagggct?cgggcagggg???1560
tggccggaac?caggaactgg?ccctgcgtgt?tggagcagag?ttgagaaggt?ggccgctggg???1620
gccgatagat?gtgctgtttt?tgagcggtgg?caccgatggg?caggatgggc?ccacagaggc???1680
tgctggggcc?tgggtcacac?ctgagcttgc?cagccaggct?gcagctgagg?gcctggacat???1740
agccaccttc?ctagcccaca?atgactcaca?taccttcttc?tgctgcctcc?agggtggggc???1800
acacctgctg?cacacaggga?tgacaggtac?caatgtcatg?gacacccacc?tcttgttcct???1860
gcggcctcgg?tgatggcata?ggtcacattt?tgggagttca?gaggaggcct?acaagggcaa???1920
gccagactgg?cagatggggg?cttcccccta?cccctgagga?tgaggacaag?cccctcggcc???1980
agttcagcgt?tcccgtgctt?ctcccttggg?cagcctctct?cttgagcccc?tcaccctgtt???2040
tctttctgtg?aagcgagaat?gtctgaaaat?aaataggacc?atgccatggg?aaaaaaaaaa???2100
aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa???2160
aaaaacggca?cgaggcggca?cgaggcaagc?ttgtagtcag?aagaactcgc?ctgggaggga???2220
tcgctcctgg?gagatccgaa?cgcgggaggg?cctggagcgc?ggcggcagct?ggatagcatt???2280
cgggcccatc?cgaataagcg?gtaaatggat?catttctggg?gcctccctga?ctcctcttcc???2340
agagcctcct?accgacctgc?tctgtcccct?ccttccttca?tgccctccac?tcttccctaa???2400
atgcaagtct?cctccccaag?cccatgtcct?cctcggcttc?ccctctgctc?tcctcaccgg???2460
tctcactcag?tcaacttcaa?gtctgagcct?tctctgcctg?tctccttcca?ttctcacctt???2520
tcctgtcaca?ccagatcact?cttctcccag?gctgtccctc?gtgccaaact?tttttggtaa???2580
atcttcctta?atgatctaga?aacagtctct?cccttctttc?tattccataa?tggtccttca???2640
aaaccccaac?cctcgccggg?cgcggtggct?cacgcctgta?atcccagcac?tttggaaggc???2700
cgaggtgggc?ggatcataag?gtcaggagat?cgagaccatc?ctggctaaca?aggtgaaaca???2760
ctatctctac?taaaaataca?ggaaaaaaaa?aaattagccg?ggcgtggggg?cgggcccctg???2820
tagtcccagg?tactcgggag?gctgaggcag?gagaatggcg?tgaacccggg?aggcggagct???2880
tgcaatgagc?caagatcttg?ccactgcact?ccagcctggg?cgacagagca?agactctgtc???2940
tcaaaaaaaa?aaaaagttaa?atttggccag?gcacagtggc?tcacacctgt?aatcccaaca???3000
ctttgggagg?ccaaggtgtg?ccagatagct?tgagccccag?agtttgagag?cagtctgggc???3060
aacatagtga?gaccttcttg?agaccttctt?ctctacaaaa?aatttaaaaa?ttagccagga???3120
acctgtagtt?ccagctactc?gggaggctga?ggtgggagaa?tcacctgagc?ccaggaggtc???3180
aaggctgcag?tgagctgtgt?tcctgccact?gtactccagc?ctgggtgaca?gtaagacctt???3240
gtaaaaaaaa?aaaaaaaaaa???????????????????????????????????????????????3260
<210>20
<211>3260
<212>DNA
＜213〉homo sapiens (Homo sapiens)
<220>
<221>CDS
<222>1310)..(1870)
<223>
<400>20
gggggatccg?tgctgccatg?gagcgtgccg?gcaagcagga?gatgctgctg?aagccacata?????60
gccgtgtcca?ggtattcgag?ggtgcggagg?acaacctccc?ggaccgcgat?gcgctgcggg????120
ctgcactggc?catccagcaa?ctggctgagg?gactcacagc?tgatgacctg?ctgctcgtgc????180
tgatctcagg?tgtggtacca?cattggccca?agactgttgg?tggggggtgc?accacctgat????240
ctctcaggtc?ttcgagagat?caggtctgag?cccccggggt?cacagggtaa?agttaggagc????300
aagggaggtg?gtgaggagcc?tggatggtga?ctcctgggtt?gccttgggct?agtcgtctgg????360
cctcccctga?gctgtggagt?cctccccctg?agctgacctt?ttcggtgaca?gtgagagggt????420
gcatggggag?cacataatgc?agggcctgac?acatccgtag?agtccttgga?aaatgtcact????480
gctattgctg?ctgtctgtac?taatgtcacc?tggggctagg?ccctcagacc?tcatggagtc????540
ctctccttga?tcttccattg?tgtgccccct?gccctgcctc?ctgttctagt?ctcttaagga????600
gtagttagtt?cttccggtgc?ctactgggac?ctggggtacc?ccaaggcagg?ggcaacctca????660
ggtggccagg?atgtggtgac?tgtacacata?ttgcatatcc?acaggggggc?accctaaccc????720
cacctttcca?gccccctttt?tctaatgtgg?gcttgtcacc?cctgcttgcc?tggttccctg????780
ggttgggata?gcccctctac?ctgataccct?cattgtcttg?agaggtgggg?gttcagctct????840
gctgcctgcc?cccatcccac?ctgtcacact?ggaggagaag?cagacactca?ctagactgct????900
ggcagcccgt?ggagccacca?tccaggagtt?gaacaccatt?cggaaggccc?tgtcccagct????960
caagggtggg?gggctggctc?aggccgccta?ccctgcccag?gtggtgagcc?tcatcctgtc???1020
agatgtggtg?gggggggggc?accctgtgga?ggtgattgcc?agtggcccca?ccgtggccag???1080
ttcccacaat?gtgcaagatt?gcctgcatat?cctcaatcgc?tacggcctcc?gtgcagccct???1140
gccacgttct?gtgaagactg?tgctgtctcg?ggccgactct?gacccccatg?ggccacacac???1200
ctgtggccat?gtcctgaatg?tgatcattgg?ctctaatgtg?ctggcgctag?ctgaggccca???1260
gcggcaggcc?gaggcactgg?gctaccaggc?tgtgttgctg?agtgcagcc?atg?caa?ggt???1318
Met?Gln?Gly
1
gat?gta?aaa?agt?atg?gcc?cag?ttc?tac?ggg?ctg?ctg?gcc?cat?gtg?gct?????1366
Asp?Val?Lys?Ser?Met?Ala?Gln?Phe?Tyr?Gly?Leu?Leu?Ala?His?Val?Ala
5???????????????????10??????????????????15
aga?acc?cgc?ctc?acc?cca?tcc?atg?gct?ggg?gct?tct?gtg?gag?gaa?gat?????1414
Arg?Thr?Arg?Leu?Thr?Pro?Ser?Met?Ala?Gly?Ala?Ser?Val?Glu?Glu?Asp
20??????????????????25??????????????????30??????????????????35
gca?cag?ctc?cat?gag?ctg?gca?gct?gag?ctt?cag?atc?cca?gac?ctg?cag?????1462
Ala?Gln?Leu?His?Glu?Leu?Ala?Ala?Glu?Leu?Gln?Ile?Pro?Asp?Leu?Gln
40??????????????????45??????????????????50
ctg?gag?gag?gct?ctg?gag?acc?atg?gca?tgg?gga?agg?ggc?cca?gtc?tgc?????1510
Leu?Glu?Glu?Ala?Leu?Glu?Thr?Met?Ala?Trp?Gly?Arg?Gly?Pro?Val?Cys
55??????????????????60??????????????????65
ctg?ctg?gct?ggt?ggc?gag?ccc?aca?gta?cag?ctg?cag?ggc?tcg?ggc?agg?????1558
Leu?Leu?Ala?Gly?Gly?Glu?Pro?Thr?Val?Gln?Leu?Gln?Gly?Ser?Gly?Arg
70??????????????????75??????????????????80
ggt?ggc?cgg?aac?cag?gaa?ctg?gcc?ctg?cgt?gtt?gga?gca?gag?ttg?aga?????1606
Gly?Gly?Arg?Asn?Gln?Glu?Leu?Ala?Leu?Arg?Val?Gly?Ala?Glu?Leu?Arg
85??????????????????90??????????????????95
agg?tgg?ccg?ctg?ggg?ccg?ata?gat?gtg?ctg?ttt?ttg?agc?ggt?ggc?acc?????1654
Arg?Trp?Pro?Leu?Gly?Pro?Ile?Asp?Val?Leu?Phe?Leu?Ser?Gly?Gly?Thr
100?????????????????105?????????????????110?????????????????115
gat?ggg?cag?gat?ggg?ccc?aca?gag?gct?gct?ggg?gcc?tgg?gtc?aca?cct?????1702
Asp?Gly?Gln?Asp?Gly?Pro?Thr?Glu?Ala?Ala?Gly?Ala?Trp?Val?Thr?Pro
120?????????????????125?????????????????130
gag?ctt?gcc?agc?cag?gct?gca?gct?gag?ggc?ctg?gac?ata?gcc?acc?ttc?????1750
Glu?Leu?Ala?Ser?Gln?Ala?Ala?Ala?Glu?Gly?Leu?Asp?Ile?Ala?Thr?Phe
135?????????????????140?????????????????145
cta?gcc?cac?aat?gac?tca?cat?acc?ttc?ttc?tgc?tgc?ctc?cag?ggt?ggg?????1798
Leu?Ala?His?Asn?Asp?Ser?His?Thr?Phe?Phe?Cys?Cys?Leu?Gln?Gly?Gly
150?????????????????155?????????????????160
gca?cac?ctg?ctg?cac?aca?ggg?atg?aca?ggt?acc?aat?gtc?atg?gac?acc?????1846
Ala?His?Leu?Leu?His?Thr?Gly?Met?Thr?Gly?Thr?Asn?Val?Met?Asp?Thr
165?????????????????170?????????????????175
cac?ctc?ttg?ttc?ctg?cgg?cct?cgg?tgatggcata?ggtcacattt?tgggagttca????1900
His?Leu?Leu?Phe?Leu?Arg?Pro?Arg
180?????????????????185
gaggaggcct?acaagggcaa?gccagactgg?cagatggggg?cttcccccta?cccctgagga???1960
tgaggacaag?cccctcggcc?agttcagcgt?tcccgtgctt?ctcccttggg?cagcctctct???2020
cttgagcccc?tcaccctgtt?tctttctgtg?aagcgagaat?gtctgaaaat?aaataggacc???2080
atgccatggg?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa???2140
aaaaaaaaaa?aaaaaaaaaa?aaaaacggca?cgaggcggca?cgaggcaagc?ttgtagtcag???2200
aagaactcgc?ctgggaggga?tcgctcctgg?gagatccgaa?cgcgggaggg?cctggagcgc???2260
ggcggcagct?ggatagcatt?cgggcccatc?cgaataagcg?gtaaatggat?catttctggg???2320
gcctccctga?ctcctcttcc?agagcctcct?accgacctgc?tctgtcccct?ccttccttca???2380
tgccctccac?tcttccctaa?atgcaagtct?cctccccaag?cccatgtcct?cctcggcttc???2440
ccctctgctc?tcctcaccgg?tctcactcag?tcaacttcaa?gtctgagcct?tctctgcctg???2500
tctccttcca?ttctcacctt?tcctgtcaca?ccagatcact?cttctcccag?gctgtccctc???2560
gtgccaaact?tttttggtaa?atcttcctta?atgatctaga?aacagtctct?cccttctttc???2620
tattccataa?tggtccttca?aaaccccaac?cctcgccggg?cgcggtggct?cacgcctgta???2680
atcccagcac?tttggaaggc?cgaggtgggc?ggatcataag?gtcaggagat?cgagaccatc???2740
ctggctaaca?aggtgaaaca?ctatctctac?taaaaataca?ggaaaaaaaa?aaattagccg???2800
ggcgtggggg?cgggcccctg?tagtcccagg?tactcgggag?gctgaggcag?gagaatggcg???2860
tgaacccggg?aggcggagct?tgcaatgagc?caagatcttg?ccactgcact?ccagcctggg???2920
cgacagagca?agactctgtc?tcaaaaaaaa?aaaaagttaa?atttggccag?gcacagtggc???2980
tcacacctgt?aatcccaaca?ctttgggagg?ccaaggtgtg?ccagatagct?tgagccccag???3040
agtttgagag?cagtctgggc?aacatagtga?gaccttcttg?agaccttctt?ctctacaaaa???3100
aatttaaaaa?ttagccagga?acctgtagtt?ccagctactc?gggaggctga?ggtgggagaa???3160
tcacctgagc?ccaggaggtc?aaggctgcag?tgagctgtgt?tcctgccact?gtactccagc???3220
ctgggtgaca?gtaagacctt?gtaaaaaaaa?aaaaaaaaaa?????????????????????????3260
<210>21
<211>187
<212>PRT
＜213〉homo sapiens (Homo sapiens)
<400>21
Met?Gln?Gly?Asp?Val?Lys?Ser?Met?Ala?Gln?Phe?Tyr?Gly?Leu?Leu?Ala
1???????????????5???????????????????10??????????????????15
His?Val?Ala?Arg?Thr?Arg?Leu?Thr?Pro?Ser?Met?Ala?Gly?Ala?Ser?Val
20??????????????????25??????????????????30
Glu?Glu?Asp?Ala?Gln?Leu?His?Glu?Leu?Ala?Ala?Glu?Leu?Gln?Ile?Pro
35??????????????????40??????????????????45
Asp?Leu?Gln?Leu?Glu?Glu?Ala?Leu?Glu?Thr?Met?Ala?Trp?Gly?Arg?Gly
50??????????????????55??????????????????60
Pro?Val?Cys?Leu?Leu?Ala?Gly?Gly?Glu?Pro?Thr?Val?Gln?Leu?Gln?Gly
65??????????????????70??????????????????75??????????????????80
Ser?Gly?Arg?Gly?Gly?Arg?Asn?Gln?Glu?Leu?Ala?Leu?Arg?Val?Gly?Ala
85??????????????????90??????????????????95
Glu?Leu?Arg?Arg?Trp?Pro?Leu?Gly?Pro?Ile?Asp?Val?Leu?Phe?Leu?Ser
100?????????????????105?????????????????110
Gly?Gly?Thr?Asp?Gly?Gln?Asp?Gly?Pro?Thr?Glu?Ala?Ala?Gly?Ala?Trp
115?????????????????120?????????????????125
Val?Thr?Pro?Glu?Leu?Ala?Ser?Gln?Ala?Ala?Ala?Glu?Gly?Leu?Asp?Ile
130?????????????????135?????????????????140
Ala?Thr?Phe?Leu?Ala?His?Asn?Asp?Ser?His?Thr?Phe?Phe?Cys?Cys?Leu
145?????????????????150?????????????????155?????????????????160
Gln?Gly?Gly?Ala?His?Leu?Leu?His?Thr?Gly?Met?Thr?Gly?Thr?Asn?Val
165?????????????????170?????????????????175
Met?Asp?Thr?His?Leu?Leu?Phe?Leu?Arg?Pro?Arg
180?????????????????185
<210>22
<211>2692
<212>DNA
＜213〉homo sapiens (Homo sapiens)
<400>22
gcggaagcgg?cggccgggcc?gccctgcgcc?cgggcggggc?cctgcggtgt?ggccgtggct?????60
tgttcctgcc?gctttcgcac?cctgcggccc?cccacccagt?gcagcagtgc?gggcgggcgt????120
gagcctcggt?gcaccaggag?gcccttcccg?cgggaggcgc?tgggctcgcg?ctaattgggg????180
cggggggggg?ggcggcgggg?gaggagggaa?ctggcgcgcg?gcttggtttc?cattagagac????240
gcaaagtttc?tgctccggga?ggaggcggcg?gcgccgcggg?ctcgtcgcct?gggggagcag????300
aagcgggtgg?gaggtgcggg?tggccttggc?cgcagccctg?gtgcgcgggg?gccgggggtg????360
gtgaccctcc?tggccgagga?ggggcggcgt?ccagacgccc?gctcgggggc?cgccttcccc????420
cccacgcctg?cccccgggca?cgcgccctgc?ccggtccctc?gccccgcgcc?acttccagtc????480
cgcagagaga?tgccctccac?gtttctgctt?tctctgcagc?ctctagattg?ccagatgcga????540
ctgtgcgcct?cgctgggtgt?gttttccaca?gccccttcct?cctcggcgtg?cagggctgac????600
atcaccgact?gcgtttctgg?tttggcgggt?ggggagatgg?ttccccgcag?ggttctggta????660
cacctttgcc?cccagggcta?gcgccatttg?ggggaggagg?ttttcgttgt?cgagaaagtt????720
ggatgctcct?ggtaacccct?ctaacaagag?agttctgtag?cgaggtggga?ctgttctccc????780
cataaggtga?cagtttctct?tgcgaggtgt?ggcagcgctt?cctgttgtac?aagacagatg????840
ttgccttggc?gttacgtaaa?tcatcgtgtc?tccgtcattt?aaagaaagcc?aatttttagt????900
gattgaggta?gaaagaaaga?tccgtttata?atttgtaaaa?acaaattttc?acccagaatc????960
aatatattgg?aacaccattc?ctactgttaa?agttttcact?taagagcata?aacttcatca???1020
gctttctatt?aggacttatt?ttgtaattgg?cttcttaggc?atccttcttt?aaaagagaaa???1080
tccacgttag?ctctccttga?ggtctcgagt?tccctcggct?ggaggcacag?gttcagtgga???1140
gaccaaataa?tgcaggtgaa?ttaccttcgt?ggccattact?gcctcgaacg?aagtgtgttt???1200
attaagaaca?gttcttatgt?cattcttaag?gtaggtaggg?ttaatactct?ccagcaaatt???1260
tagtagatac?tgtttgccag?aaaagagagg?agtatatata?gtttgataat?tattgtgtag???1320
ttttctgtgt?acttaatttt?tgcagttttg?taacacttca?tttgtaagat?ggtaccattt???1380
tttcctggct?tctgaatcat?aggatagttt?gacccagggc?attagccatt?gtaatggtag???1440
gcttttaaca?aataactgcc?taatttaaag?gattggaaag?catttgttac?atggaaatga???1500
agttggtggc?gtacccagtt?gctgtatctt?tattttttgt?acttaattat?ttctcataaa???1560
atggatataa?aagcctgtta?atccaaccca?atgccattat?gtaacgccag?tttggagatt???1620
tcgagggcct?ggagcagtgc?gcaaggtgcg?ctgaaagcct?gcccctggat?gagatcctta???1680
tcctggctgt?gatggcagtg?gcagtgggct?gggtcccttg?ttgagtggaa?agggggactg???1740
cggtgtccat?ggtgcagtag?gtggcgctct?tctgtcttag?agcctgccgc?cactgcagct???1800
ggtgccaagg?ggccttctgc?cactagaggt?gccatttttc?acatgatgaa?cttagcctag???1860
ttagatcgca?gagcaagctg?taagccatgg?gcccagaaaa?gaaaacttga?agtgagcaga???1920
tgttgtcact?tccttgtaat?cctttgttaa?aatagcataa?ggagttttct?ttattctatt???1980
tactttcatt?aaatgaccgt?gctacaggtt?tcaaaggatt?ttaagattga?tttttgaaag???2040
atcacaatat?taaaagtata?actggaaaat?ctatgttgaa?atcaaccaaa?catgtcgtgg???2100
actgaatgat?aaccttttct?ttcttcatat?aggctgatca?gctgaccgaa?gaacagattg???2160
ctgaattcaa?ggaagccttc?tccctatttg?ataaagatgg?cgatggcacc?atcacaacaa???2220
aggaacttgg?aactgtcatg?aggtcactgg?gtcagaaccc?aacagaagct?gaattgcagg???2280
atatgatcaa?tgaagtggat?gctgatggta?atggcaccat?tgacttcccc?gaatttttga???2340
ctatgatggc?tagaaaaatg?aaagatacag?atagtgaaga?agaaatccgt?gaggcattcc???2400
gagtctttga?caaggatggc?aatggttata?tcagtgcagc?agaactacgt?cacgtcatga???2460
caaacttagg?agaaaaacta?acagatgaag?aagtagatga?aatgatcaga?gaagcagata???2520
ttgatggaga?cggacaagtc?aactatgaag?aattcgtaca?gatgatgact?gcaaaatgaa???2580
gacctacttt?caactccttt?ttcccccctc?tagaagaatc?aaattgaatc?ttttacttac???2640
ctcttgccaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aa???????????2692
<210>23
<211>2692
<212>DNA
＜213〉homo sapiens (Homo sapiens)
<220>
<221>CDS
<222>(2238)..(2576)
<223>
<400>23
gcggaagcgg?cggccgggcc?gccctgcgcc?cgggcggggc?cctgcggtgt?ggccgtggct?????60
tgttcctgcc?gctttcgcac?cctgcggccc?cccacccagt?gcagcagtgc?gggcgggcgt????120
gagcctcggt?gcaccaggag?gcccttcccg?cgggaggcgc?tgggctcgcg?ctaattgggg????180
cggggggggg?ggcggcgggg?gaggagggaa?ctggcgcgcg?gcttggtttc?cattagagac????240
gcaaagtttc?tgctccggga?ggaggcggcg?gcgccgcggg?ctcgtcgcct?gggggagcag????300
aagcgggtgg?gaggtgcggg?tggccttggc?cgcagccctg?gtgcgcgggg?gccgggggtg????360
gtgaccctcc?tggccgagga?ggggcggcgt?ccagacgccc?gctcgggggc?cgccttcccc????420
cccacgcctg?cccccgggca?cgcgccctgc?ccggtccctc?gccccgcgcc?acttccagtc????480
cgcagagaga?tgccctccac?gtttctgctt?tctctgcagc?ctctagattg?ccagatgcga????540
ctgtgcgcct?cgctgggtgt?gttttccaca?gccccttcct?cctcggcgtg?cagggctgac????600
atcaccgact?gcgtttctgg?tttggcgggt?ggggagatgg?ttccccgcag?ggttctggta????660
cacctttgcc?cccagggcta?gcgccatttg?ggggaggagg?ttttcgttgt?cgagaaagtt????720
ggatgctcct?ggtaacccct?ctaacaagag?agttctgtag?cgaggtggga?ctgttctccc????780
cataaggtga?cagtttctct?tgcgaggtgt?ggcagcgctt?cctgttgtac?aagacagatg????840
ttgccttggc?gttacgtaaa?tcatcgtgtc?tccgtcattt?aaagaaagcc?aatttttagt????900
gattgaggta?gaaagaaaga?tccgtttata?atttgtaaaa?acaaattttc?acccagaatc????960
aatatattgg?aacaccattc?ctactgttaa?agttttcact?taagagcata?aacttcatca???1020
gctttctatt?aggacttatt?ttgtaattgg?cttcttaggc?atccttcttt?aaaagagaaa???1080
tccacgttag?ctctccttga?ggtctcgagt?tccctcggct?ggaggcacag?gttcagtgga???1140
gaccaaataa?tgcaggtgaa?ttaccttcgt?ggccattact?gcctcgaacg?aagtgtgttt???1200
attaagaaca?gttcttatgt?cattcttaag?gtaggtaggg?ttaatactct?ccagcaaatt???1260
tagtagatac?tgtttgccag?aaaagagagg?agtatatata?gtttgataat?tattgtgtag???1320
ttttctgtgt?acttaatttt?tgcagttttg?taacacttca?tttgtaagat?ggtaccattt???1380
tttcctggct?tctgaatcat?aggatagttt?gacccagggc?attagccatt?gtaatggtag???1440
gcttttaaca?aataactgcc?taatttaaag?gattggaaag?catttgttac?atggaaatga???1500
agttggtggc?gtacccagtt?gctgtatctt?tattttttgt?acttaattat?ttctcataaa???1560
atggatataa?aagcctgtta?atccaaccca?atgccattat?gtaacgccag?tttggagatt???1620
tcgagggcct?ggagcagtgc?gcaaggtgcg?ctgaaagcct?gcccctggat?gagatcctta???1680
tcctggctgt?gatggcagtg?gcagtgggct?gggtcccttg?ttgagtggaa?agggggactg???1740
cggtgtccat?ggtgcagtag?gtggcgctct?tctgtcttag?agcctgccgc?cactgcagct???1800
ggtgccaagg?ggccttctgc?cactagaggt?gccatttttc?acatgatgaa?cttagcctag???1860
ttagatcgca?gagcaagctg?taagccatgg?gcccagaaaa?gaaaacttga?agtgagcaga???1920
tgttgtcact?tccttgtaat?cctttgttaa?aatagcataa?ggagttttct?ttattctatt???1980
tactttcatt?aaatgaccgt?gctacaggtt?tcaaaggatt?ttaagattga?tttttgaaag???2040
atcacaatat?taaaagtata?actggaaaat?ctatgttgaa?atcaaccaaa?catgtcgtgg???2100
actgaatgat?aaccttttct?ttcttcatat?aggctgatca?gctgaccgaa?gaacagattg???2160
ctgaattcaa?ggaagccttc?tccctatttg?ataaagatgg?cgatggcacc?atcacaacaa???2220
aggaacttgg?aactgtc?atg?agg?tca?ctg?ggt?cag?aac?cca?aca?gaa?gct??????2270
Met?Arg?Ser?Leu?Gly?Gln?Asn?Pro?Thr?Glu?Ala
1???????????????5???????????????????10
gaa?ttg?cag?gat?atg?atc?aat?gaa?gtg?gat?gct?gat?ggt?aat?ggc?acc?????2318
Glu?Leu?Gln?Asp?Met?Ile?Asn?Glu?Val?Asp?Ala?Asp?Gly?Asn?Gly?Thr
15??????????????????20??????????????????25
att?gac?ttc?ccc?gaa?ttt?ttg?act?atg?atg?gct?aga?aaa?atg?aaa?gat?????2366
Ile?Asp?Phe?Pro?Glu?Phe?Leu?Thr?Met?Met?Ala?Arg?Lys?Met?Lys?Asp
30??????????????????35??????????????????40
aca?gat?agt?gaa?gaa?gaa?atc?cgt?gag?gca?ttc?cga?gtc?ttt?gac?aag?????2414
Thr?Asp?Ser?Glu?Glu?Glu?Ile?Arg?Glu?Ala?Phe?Arg?Val?Phe?Asp?Lys
45??????????????????50??????????????????55
gat?ggc?aat?ggt?tat?atc?agt?gca?gca?gaa?cta?cgt?cac?gtc?atg?aca?????2462
Asp?Gly?Asn?Gly?Tyr?Ile?Ser?Ala?Ala?Glu?Leu?Arg?His?Val?Met?Thr
60??????????????????65??????????????????70??????????????????75
aac?tta?gga?gaa?aaa?eta?aca?gat?gaa?gaa?gta?gat?gaa?atg?atc?aga?????2510
Asn?Leu?Gly?Glu?Lys?Leu?Thr?Asp?Glu?Glu?Val?Asp?Glu?Met?Ile?Arg
80??????????????????85??????????????????90
gaa?gca?gat?att?gat?gga?gac?gga?caa?gtc?aac?tat?gaa?gaa?ttc?gta?????2558
Glu?Ala?Asp?Ile?Asp?Gly?Asp?Gly?Gln?Val?Asn?Tyr?Glu?Glu?Phe?Val
95??????????????????100?????????????????105
cag?atg?atg?act?gca?aaa?tgaagaccta?ctttcaactc?ctttttcccc????????????2606
Gln?Met?Met?Thr?Ala?Lys
110
cctctagaag?aatcaaattg?aatcttttac?ttacctcttg?ccaaaaaaaa?aaaaaaaaaa???2666
aaaaaaaaaa?aaaaaaaaaa?aaaaaa????????????????????????????????????????2692
<210>24
<211>113
<212>PRT
＜213〉homo sapiens (Homo sapiens)
<400>24
Met?Arg?Ser?Leu?Gly?Gln?Asn?Pro?Thr?Glu?Ala?Glu?Leu?Gln?Asp?Met
1???????????????5???????????????????10??????????????????15
Ile?Asn?Glu?Val?Asp?Ala?Asp?Gly?Asn?Gly?Thr?Ile?Asp?Phe?Pro?Glu
20??????????????????25??????????????????30
Phe?Leu?Thr?Met?Met?Ala?Arg?Lys?Met?Lys?Asp?Thr?Asp?Ser?Glu?Glu
35??????????????????40??????????????????45
Glu?Ile?Arg?Glu?Ala?Phe?Arg?Val?Phe?Asp?Lys?Asp?Gly?Asn?Gly?Tyr
50??????????????????55??????????????????60
Ile?Ser?Ala?Ala?Glu?Leu?Arg?His?Val?Met?Thr?Asn?Leu?Gly?Glu?Lys
65??????????????????70??????????????????75??????????????????80
Leu?Thr?Asp?Glu?Glu?Val?Asp?Glu?Met?Ile?Arg?Glu?Ala?Asp?Ile?Asp
85??????????????????90??????????????????95
Gly?Asp?Gly?Gln?Val?Asn?Tyr?Glu?Glu?Phe?Val?Gln?Met?Met?Thr?Ala
100?????????????????105?????????????????110
Lys
<210>25
<211>2769
<212>DNA
＜213〉homo sapiens (Homo sapiens)
<400>25
gtcttggtct?tctggcctgg?cggcgatcgt?cggccagttt?atccctcgga?gttgcactgg?????60
cagacacgcc?gctactttgt?agcgggtttc?gggcgggcca?cgcgtgcggc?gacaggaacc????120
caaccccggc?cgaccttggg?ctccaggaat?tcgttgtcta?cgtctgcgga?ggtgcggcag????180
cctcagtttt?aagcgcagga?aggcaataga?ggctgtgctc?tgaacctttc?cctcacgatg????240
atcttctgcc?tgttgaccat?cctggtgtct?ttcccatgtg?gccctgtatg?gttggcatgg????300
ttcctgtctt?taggacttgt?gatcaagtat?atctgaaagt?ttttagaaga?aaagatgtgt????360
tgtaacctcc?ctggaaaaag?aagagacctt?atgaagtact?gctaaccacc?tacatagtca????420
tcaaaataaa?attcctgaat?ttgtacaagc?cacaggaagc?tagattgaga?tcattatatg????480
acaactggaa?ggccaaggct?atgggttacc?tcaaattgag?gaattttggc?acctactcac????540
aggctccatg?agcagatgaa?gtagacagct?ttactcagta?tctcagacca?agaacttcat????600
ctccatctcc?aactagctga?aacatcttcc?ctcctcaacc?tggaaaattc?tctgacttag????660
aaatttaaac?aaaaccctcc?cctttcattg?aatctccatt?gtctggagtt?tgcttgtttt????720
aatctagcct?gttcctccac?tatgggctcc?ctttcaaact?atgccctgct?tcaactaacc????780
cttactgctt?ttttgacaat?tctagtacaa?cctcagcacc?tgcttgctcc?agttttccgg????840
acactatcta?tcttgactaa?tcagtctaat?tgctggttat?gtgaacatct?agataatgca????900
gaacaacccg?aactagtttt?tgttcctgcc?agtgcaagca?cctggtggac?ctattctgga????960
caatggatgt?atgaaagggt?gtggtatcca?caagcagaag?tacagaatca?ctctacttcc???1020
tcctatcgta?aagtgacttg?gcactgggaa?gcctccatgg?aagctcaagg?tctatccttt???1080
gctcaagtaa?ggttattgga?gggaaatttt?tctctttgcg?tagaaaataa?aaatggcagt???1140
ggacccttcc?taggtaatat?acctaaacaa?tactgtaatc?aaatactatg?gtttgattct???1200
acagatggca?ccttcatgcc?ctctatagat?gttacaaatg?aatccaggaa?cgatgatgat???1260
gatacaagtg?tttgcctagg?cactagacaa?tgttcctggt?ttgcaggttg?cacaaaccgg???1320
acctggaaca?gctcagctgt?tcccttgatt?ggtctgccca?atacccaaga?ctacaaatgg???1380
gtagatcgaa?attctggatt?gacctggtca?ggtaatgaca?cctgtctcta?tagctgccaa???1440
aaccaaacca?aaggccttct?gtaccagcta?tttcgcaacc?tattttgctc?ttatggcctg???1500
acagaggcac?atgggaaatg?gagatgtgca?gatgccagca?taactaatga?caaaggtcat???1560
gatggacacc?ggacccccac?ctggtggctc?acaggttcca?atctgacctt?gtctgtgaac???1620
aactctggcc?tctttttttt?gtgcggcaat?ggggtgtaca?aagggtttcc?acctaaatgg???1680
tctgggcgat?gtggacttgg?gtatcttgta?ccttccctca?ccagatacct?caccttaaat???1740
gctagccaaa?ttacaaacct?gagatccttc?attcataaag?taacaccgca?tagatgcacc???1800
caaggagaca?cagacaatcc?acctctgtat?tgcaacccca?aggacaattc?aacaataagg???1860
gccctttttc?caagtttggg?aacttatgat?ttagaaaagg?caattctaaa?catttccaaa???1920
gcaatggaac?aggaattcag?tgccactaag?cagaccttgg?aagcacacca?atcaaaagtt???1980
agcagtttag?cctctgcatc?ccgaaaggat?catgtcttgg?atataccgac?cacccaacga???2040
caaacggctt?gtggaactgt?tggcaaacag?tgttgcctct?atataaatta?ttcggaagaa???2100
ataaagtcta?atatacagcg?tctccacgaa?gcatccgaga?acctgaagaa?tgtaccgtta???2160
cttgattggc?aaggcatatt?tgcaaaagtg?ggagactggt?tcagatcatg?gggctatgtg???2220
cttttaattg?ttcttttctg?cttattcatc?tttgttttaa?tctatgttcg?tgtctttcgc???2280
aaatctcgca?gatcccttaa?ctcccaacct?ctgaacctag?ccttatctcc?acagcaatca???2340
gcacagctcc?ttgtcagtga?aacttcatgt?caagtttcaa?atagggcaat?gaagggacta???2400
acaacccatc?aatatgacac?aagtctactt?tgagaatatt?tgaacaaaca?gcagctgcag???2460
acaaaaagcc?ttagctaaac?tttgatgagt?aaagcaggtc?ttaccgagaa?ttcagctgcc???2520
aaaaccctcc?tctgagtgtt?cctcttataa?gggcacttag?cactaggacc?tcccaaggta???2580
ttgtaaataa?gccttatcag?aactttttgt?agtttcattc?tgaagcctta?agacacacac???2640
cataaagctg?atctgtaaac?ccttacccct?tgctgttcag?agagctactc?tttgtagtgt???2700
tcttgcatgc?atatataata?aatgtttttt?ctattgatct?gttaaaaaaa?aaaaaaaaaa???2760
aaaaaaaaa???????????????????????????????????????????????????????????2769
<210>26
<211>2769
<212>DNA
＜213〉homo sapiens (Homo sapiens)
<220>
<221>CDS
<222>(742)..(2430)
<223>
<400>26
gtcttggtct?tctggcctgg?cggcgatcgt?cggccagttt?atccctcgga?gttgcactgg?????60
cagacacgcc?gctactttgt?agcgggtttc?gggcgggcca?cgcgtgcggc?gacaggaacc????120
caaccccggc?cgaccttggg?ctccaggaat?tcgttgtcta?cgtctgcgga?ggtgcggcag????180
cctcagtttt?aagcgcagga?aggcaataga?ggctgtgctc?tgaacctttc?cctcacgatg????240
atcttctgcc?tgttgaccat?cctggtgtct?ttcccatgtg?gccctgtatg?gttggcatgg????300
ttcctgtctt?taggacttgt?gatcaagtat?atctgaaagt?ttttagaaga?aaagatgtgt????360
tgtaacctcc?ctggaaaaag?aagagacctt?atgaagtact?gctaaccacc?tacatagtca????420
tcaaaataaa?attcctgaat?ttgtacaagc?cacaggaagc?tagattgaga?tcattatatg????480
acaactggaa?ggccaaggct?atgggttacc?tcaaattgag?gaattttggc?acctactcac????540
aggctccatg?agcagatgaa?gtagacagct?ttactcagta?tctcagacca?agaacttcat????600
ctccatctcc?aactagctga?aacatcttcc?ctcctcaacc?tggaaaattc?tctgacttag????660
aaatttaaac?aaaaccctcc?cctttcattg?aatctccatt?gtctggagtt?tgcttgtttt????720
aatctagcct?gttcctccac?t?atg?ggc?tcc?ctt?tca?aac?tat?gcc?ctg?ctt??????771
Met?Gly?Ser?Leu?Ser?Asn?Tyr?Ala?Leu?Leu
1???????????????5???????????????????10
caa?cta?acc?ctt?act?gct?ttt?ttg?aca?att?cta?gta?caa?cct?cag?cac??????819
Gln?Leu?Thr?Leu?Thr?Ala?Phe?Leu?Thr?Ile?Leu?Val?Gln?Pro?Gln?His
15??????????????????20??????????????????25
ctg?ctt?gct?cca?gtt?ttc?cgg?aca?cta?tct?atc?ttg?act?aat?cag?tct??????867
Leu?Leu?Ala?Pro?Val?Phe?Arg?Thr?Leu?Ser?Ile?Leu?Thr?Asn?Gln?Ser
30??????????????????35??????????????????40
aat?tgc?tgg?tta?tgt?gaa?cat?cta?gat?aat?gca?gaa?caa?ccc?gaa?cta??????915
Asn?Cys?Trp?Leu?Cys?Glu?His?Leu?Asp?Asn?Ala?Glu?Gln?Pro?Glu?Leu
45??????????????????50??????????????????55
gtt?ttt?gtt?cct?gcc?agt?gca?agc?acc?tgg?tgg?acc?tat?tct?gga?caa??????963
Val?Phe?Val?Pro?Ala?Ser?Ala?Ser?Thr?Trp?Trp?Thr?Tyr?Ser?Gly?Gln
60??????????????????65??????????????????70
tgg?atg?tat?gaa?agg?gtg?tgg?tat?cca?caa?gca?gaa?gta?cag?aat?cac?????1011
Trp?Met?Tyr?Glu?Arg?Val?Trp?Tyr?Pro?Gln?Ala?Glu?Val?Gln?Asn?His
75??????????????????80??????????????????85??????????????????90
tct?act?tcc?tcc?tat?cgt?aaa?gtg?act?tgg?cac?tgg?gaa?gcc?tcc?atg?????1059
Ser?Thr?Ser?Ser?Tyr?Arg?Lys?Val?Thr?Trp?His?Trp?Glu?Ala?Ser?Met
95??????????????????100?????????????????105
gaa?gct?caa?ggt?cta?tcc?ttt?gct?caa?gta?agg?tta?ttg?gag?gga?aat?????1107
Glu?Ala?Gln?Gly?Leu?Ser?Phe?Ala?Gln?Val?Arg?Leu?Leu?Glu?Gly?Asn
110?????????????????115?????????????????120
ttt?tct?ctt?tgc?gta?gaa?aat?aaa?aat?ggc?agt?gga?ccc?ttc?cta?ggt?????1155
Phe?Ser?Leu?Cys?Val?Glu?Asn?Lys?Asn?Gly?Ser?Gly?Pro?Phe?Leu?Gly
125?????????????????130?????????????????135
aat?ata?cct?aaa?caa?tac?tgt?aat?caa?ata?cta?tgg?ttt?gat?tct?aca?????1203
Asn?Ile?Pro?Lys?Gln?Tyr?Cys?Asn?Gln?Ile?Leu?Trp?Phe?Asp?Ser?Thr
140?????????????????145?????????????????150
gat?ggc?acc?ttc?atg?ccc?tct?ata?gat?gtt?aca?aat?gaa?tcc?agg?aac?????1251
Asp?Gly?Thr?Phe?Met?Pro?Ser?Ile?Asp?Val?Thr?Asn?Glu?Ser?Arg?Asn
155?????????????????160?????????????????165?????????????????170
gat?gat?gat?gat?aca?agt?gtt?tgc?cta?ggc?act?aga?caa?tgt?tcc?tgg?????1299
Asp?Asp?Asp?Asp?Thr?Ser?Val?Cys?Leu?Gly?Thr?Arg?Gln?Cys?Ser?Trp
175?????????????????180?????????????????185
ttt?gca?ggt?tgc?aca?aac?cgg?acc?tgg?aac?agc?tca?gct?gtt?ccc?ttg?????1347
Phe?Ala?Gly?Cys?Thr?Asn?Arg?Thr?Trp?Asn?Ser?Ser?Ala?Val?Pro?Leu
190?????????????????195?????????????????200
att?ggt?ctg?ccc?aat?acc?caa?gac?tac?aaa?tgg?gta?gat?cga?aat?tct?????1395
Ile?Gly?Leu?Pro?Asn?Thr?Gln?Asp?Tyr?Lys?Trp?Val?Asp?Arg?Asn?Ser
205?????????????????210?????????????????215
gga?ttg?acc?tgg?tca?ggt?aat?gac?acc?tgt?ctc?tat?agc?tgc?caa?aac?????1443
Gly?Leu?Thr?Trp?Ser?Gly?Asn?Asp?Thr?Cys?Leu?Tyr?Ser?Cys?Gln?Asn
220?????????????????225?????????????????230
caa?acc?aaa?ggc?ctt?ctg?tac?cag?cta?ttt?cgc?aac?cta?ttt?tgc?tct?????1491
Gln?Thr?Lys?Gly?Leu?Leu?Tyr?Gln?Leu?Phe?Arg?Asn?Leu?Phe?Cys?Ser
235?????????????????240?????????????????245?????????????????250
tat?ggc?ctg?aca?gag?gca?cat?ggg?aaa?tgg?aga?tgt?gca?gat?gcc?agc?????1539
Tyr?Gly?Leu?Thr?Glu?Ala?His?Gly?Lys?Trp?Arg?Cys?Ala?Asp?Ala?Ser
255?????????????????260?????????????????265
ata?act?aat?gac?aaa?ggt?cat?gat?gga?cac?cgg?acc?ccc?acc?tgg?tgg?????1587
Ile?Thr?Asn?Asp?Lys?Gly?His?Asp?Gly?His?Arg?Thr?Pro?Thr?Trp?Trp
270?????????????????275?????????????????280
ctc?aca?ggt?tcc?aat?ctg?acc?ttg?tct?gtg?aac?aac?tct?ggc?ctc?ttt?????1635
Leu?Thr?Gly?Ser?Asn?Leu?Thr?Leu?Ser?Val?Asn?Asn?Ser?Gly?Leu?Phe
285?????????????????290?????????????????295
ttt?ttg?tgc?ggc?aat?ggg?gtg?tac?aaa?ggg?ttt?cca?cct?aaa?tgg?tct?????1683
Phe?Leu?Cys?Gly?Asn?Gly?Val?Tyr?Lys?Gly?Phe?Pro?Pro?Lys?Trp?Ser
300?????????????????305?????????????????310
ggg?cga?tgt?gga?ctt?ggg?tat?ctt?gta?cct?tcc?ctc?acc?aga?tac?ctc?????1731
Gly?Arg?Cys?Gly?Leu?Gly?Tyr?Leu?Val?Pro?Ser?Leu?Thr?Arg?Tyr?Leu
315?????????????????320?????????????????325?????????????????330
acc?tta?aat?gct?agc?caa?att?aca?aac?ctg?aga?tcc?ttc?att?cat?aaa?????1779
Thr?Leu?Asn?Ala?Ser?Gln?Ile?Thr?Asn?Leu?Arg?Ser?Phe?Ile?His?Lys
335?????????????????340?????????????????345
gta?aca?ccg?cat?aga?tgc?acc?caa?gga?gac?aca?gac?aat?cca?cct?ctg?????1827
Val?Thr?Pro?His?Arg?Cys?Thr?Gln?Gly?Asp?Thr?Asp?Asn?Pro?Pro?Leu
350?????????????????355?????????????????360
tat?tgc?aac?ccc?aag?gac?aat?tca?aca?ata?agg?gcc?ctt?ttt?cca?agt?????1875
Tyr?Cys?Asn?Pro?Lys?Asp?Asn?Ser?Thr?Ile?Arg?Ala?Leu?Phe?Pro?Ser
365?????????????????370?????????????????375
ttg?gga?act?tat?gat?tta?gaa?aag?gca?att?cta?aac?att?tcc?aaa?gca?????1923
Leu?Gly?Thr?Tyr?Asp?Leu?Glu?Lys?Ala?Ile?Leu?Asn?Ile?Ser?Lys?Ala
380?????????????????385?????????????????390
atg?gaa?cag?gaa?ttc?agt?gcc?act?aag?cag?acc?ttg?gaa?gca?cac?caa?????1971
Met?Glu?Gln?Glu?Phe?Ser?Ala?Thr?Lys?Gln?Thr?Leu?Glu?Ala?His?Gln
395?????????????????400?????????????????405?????????????????410
tca?aaa?gtt?agc?agt?tta?gcc?tct?gca?tcc?cga?aag?gat?cat?gtc?ttg?????2019
Ser?Lys?Val?Ser?Ser?Leu?Ala?Ser?Ala?Ser?Arg?Lys?Asp?His?Val?Leu
415?????????????????420?????????????????425
gat?ata?ccg?acc?acc?caa?cga?caa?acg?gct?tgt?gga?act?gtt?ggc?aaa?????2067
Asp?Ile?Pro?Thr?Thr?Gln?Arg?Gln?Thr?Ala?Cys?Gly?Thr?Val?Gly?Lys
430?????????????????435?????????????????440
cag?tgt?tgc?ctc?tat?ata?aat?tat?tcg?gaa?gaa?ata?aag?tct?aat?ata?????2115
Gln?Cys?Cys?Leu?Tyr?Ile?Asn?Tyr?Ser?Glu?Glu?Ile?Lys?Ser?Asn?Ile
445?????????????????450?????????????????455
cag?cgt?ctc?cac?gaa?gca?tcc?gag?aac?ctg?aag?aat?gta?ccg?tta?ctt?????2163
Gln?Arg?Leu?His?Glu?Ala?Ser?Glu?Asn?Leu?Lys?Asn?Val?Pro?Leu?Leu
460?????????????????465?????????????????470
gat?tgg?caa?ggc?ata?ttt?gca?aaa?gtg?gga?gac?tgg?ttc?aga?tca?tgg?????2211
Asp?Trp?Gln?Gly?Ile?Phe?Ala?Lys?Val?Gly?Asp?Trp?Phe?Arg?Ser?Trp
475?????????????????480?????????????????485?????????????????490
ggc?tat?gtg?ctt?tta?att?gtt?ctt?ttc?tgc?tta?ttc?atc?ttt?gtt?tta?????2259
Gly?Tyr?Val?Leu?Leu?Ile?Val?Leu?Phe?Cys?Leu?Phe?Ile?Phe?Val?Leu
495?????????????????500?????????????????505
atc?tat?gtt?cgt?gtc?ttt?cgc?aaa?tct?cgc?aga?tcc?ctt?aac?tcc?caa?????2307
Ile?Tyr?Val?Arg?Val?Phe?Arg?Lys?Ser?Arg?Arg?Ser?Leu?Asn?Ser?Gln
510?????????????????515?????????????????520
cct?ctg?aac?cta?gcc?tta?tct?cca?cag?caa?tca?gca?cag?ctc?ctt?gtc?????2355
Pro?Leu?Asn?Leu?Ala?Leu?Ser?Pro?Gln?Gln?Ser?Ala?Gln?Leu?Leu?Val
525?????????????????530?????????????????535
agt?gaa?act?tca?tgt?caa?gtt?tca?aat?agg?gca?atg?aag?gga?cta?aca?????2403
Ser?Glu?Thr?Ser?Cys?Gln?Val?Ser?Asn?Arg?Ala?Met?Lys?Gly?Leu?Thr
540?????????????????545?????????????????550
acc?cat?caa?tat?gac?aca?agt?cta?ctt?tgagaatatt?tgaacaaaca???????????2450
Thr?His?Gln?Tyr?Asp?Thr?Ser?Leu?Leu
555?????????????????560
gcagctgcag?acaaaaagcc?ttagctaaac?tttgatgagt?aaagcaggtc?ttaccgagaa???2510
ttcagctgcc?aaaaccctcc?tctgagtgtt?cctcttataa?gggcacttag?cactaggacc???2570
tcccaaggta?ttgtaaataa?gccttatcag?aactttttgt?agtttcattc?tgaagcctta???2630
agacacacac?cataaagctg?atctgtaaac?ccttacccct?tgctgttcag?agagctactc???2690
tttgtagtgt?tcttgcatgc?atatataata?aatgtttttt?ctattgatct?gttaaaaaaa???2750
aaaaaaaaaa?aaaaaaaaa????????????????????????????????????????????????2769
<210>27
<2l1>563
<212>PRT
＜213〉homo sapiens (Homo sapiens)
<400>27
Met?Gly?Ser?Leu?Ser?Asn?Tyr?Ala?Leu?Leu?Gln?Leu?Thr?Leu?Thr?Ala
1???????????????5???????????????????10??????????????????15
Phe?Leu?Thr?Ile?Leu?Val?Gln?Pro?Gln?His?Leu?Leu?Ala?Pro?Val?Phe
20??????????????????25??????????????????30
Arg?Thr?Leu?Ser?Ile?Leu?Thr?Asn?Gln?Ser?Asn?Cys?Trp?Leu?Cys?Glu
35??????????????????40??????????????????45
His?Leu?Asp?Asn?Ala?Glu?Gln?Pro?Glu?Leu?Val?Phe?Val?Pro?Ala?Ser
50??????????????????55??????????????????60
Ala?Ser?Thr?Trp?Trp?Thr?Tyr?Ser?Gly?Gln?Trp?Met?Tyr?Glu?Arg?Val
65??????????????????70??????????????????75??????????????????80
Trp?Tyr?Pro?Gln?Ala?Glu?Val?Gln?Asn?His?Ser?Thr?Ser?Ser?Tyr?Arg
85??????????????????90??????????????????95
Lys?Val?Thr?Trp?His?Trp?Glu?Ala?Ser?Met?Glu?Ala?Gln?Gly?Leu?Ser
100?????????????????105?????????????????110
Phe?Ala?Gln?Val?Arg?Leu?Leu?Glu?Gly?Asn?Phe?Ser?Leu?Cys?Val?Glu
115?????????????????120?????????????????125
Asn?Lys?Asn?Gly?Ser?Gly?Pro?Phe?Leu?Gly?Asn?Ile?Pro?Lys?Gln?Tyr
130?????????????????135?????????????????140
Cys?Asn?Gln?Ile?Leu?Trp?Phe?Asp?Ser?Thr?Asp?Gly?Thr?Phe?Met?Pro
145?????????????????150?????????????????155?????????????????160
Ser?Ile?Asp?Val?Thr?Asn?Glu?Ser?Arg?Asn?Asp?Asp?Asp?Asp?Thr?Ser
165?????????????????170?????????????????175
Val?Cys?Leu?Gly?Thr?Arg?Gln?Cys?Ser?Trp?Phe?Ala?Gly?Cys?Thr?Asn
180?????????????????185?????????????????190
Arg?Thr?Trp?Asn?Ser?Ser?Ala?Val?Pro?Leu?Ile?Gly?Leu?Pro?Asn?Thr
195?????????????????200?????????????????205
Gln?Asp?Tyr?Lys?Trp?Val?Asp?Arg?Asn?Ser?Gly?Leu?Thr?Trp?Ser?Gly
210?????????????????215?????????????????220
Asn?Asp?Thr?Cys?Leu?Tyr?Ser?Cys?Gln?Asn?Gln?Thr?Lys?Gly?Leu?Leu
225?????????????????230?????????????????235?????????????????240
Tyr?Gln?Leu?Phe?Arg?Asn?Leu?Phe?Cys?Ser?Tyr?Gly?Leu?Thr?Glu?Ala
245?????????????????250?????????????????255
His?Gly?Lys?Trp?Arg?Cys?Ala?Asp?Ala?Ser?Ile?Thr?Asn?Asp?Lys?Gly
260?????????????????265?????????????????270
His?Asp?Gly?His?Arg?Thr?Pro?Thr?Trp?Trp?Leu?Thr?Gly?Ser?Asn?Leu
275?????????????????280?????????????????285
Thr?Leu?Ser?Val?Asn?Asn?Ser?Gly?Leu?Phe?Phe?Leu?Cys?Gly?Asn?Gly
290?????????????????295?????????????????300
Val?Tyr?Lys?Gly?Phe?Pro?Pro?Lys?Trp?Ser?Gly?Arg?Cys?Gly?Leu?Gly
305?????????????????310?????????????????315?????????????????320
Tyr?Leu?Val?Pro?Ser?Leu?Thr?Arg?Tyr?Leu?Thr?Leu?Asn?Ala?Ser?Gln
325?????????????????330?????????????????335
Ile?Thr?Asn?Leu?Arg?Ser?Phe?Ile?His?Lys?Val?Thr?Pro?His?Arg?Cys
340?????????????????345?????????????????350
Thr?Gln?Gly?Asp?Thr?Asp?Asn?Pro?Pro?Leu?Tyr?Cys?Asn?Pro?Lys?Asp
355?????????????????360?????????????????365
Asn?Ser?Thr?Ile?Arg?Ala?Leu?Phe?Pro?Ser?Leu?Gly?Thr?Tyr?Asp?Leu
370?????????????????375?????????????????380
Glu?Lys?Ala?Ile?Leu?Asn?Ile?Ser?Lys?Ala?Met?Glu?Gln?Glu?Phe?Ser
385?????????????????390?????????????????395?????????????????400
Ala?Thr?Lys?Gln?Thr?Leu?Glu?Ala?His?Gln?Ser?Lys?Val?Ser?Ser?Leu
405?????????????????410?????????????????415
Ala?Ser?Ala?Ser?Arg?Lys?Asp?His?Val?Leu?Asp?Ile?Pro?Thr?Thr?Gln
420?????????????????425?????????????????430
Arg?Gln?Thr?Ala?Cys?Gly?Thr?Val?Gly?Lys?Gln?Cys?Cys?Leu?Tyr?Ile
435?????????????????440?????????????????445
Asn?Tyr?Ser?Glu?Glu?Ile?Lys?Ser?Asn?Ile?Gln?Arg?Leu?His?Glu?Ala
450?????????????????455?????????????????460
Ser?Glu?Asn?Leu?Lys?Asn?Val?Pro?Leu?Leu?Asp?Trp?Gln?Gly?Ile?Phe
465?????????????????470?????????????????475?????????????????480
Ala?Lys?Val?Gly?Asp?Trp?Phe?Arg?Ser?Trp?Gly?Tyr?Val?Leu?Leu?Ile
485?????????????????490?????????????????495
Val?Leu?Phe?Cys?Leu?Phe?Ile?Phe?Val?Leu?Ile?Tyr?Val?Arg?Val?Phe
500?????????????????505?????????????????510
Arg?Lys?Ser?Arg?Arg?Ser?Leu?Asn?Ser?Gln?Pro?Leu?Asn?Leu?Ala?Leu
515?????????????????520?????????????????525
Ser?Pro?Gln?Gln?Ser?Ala?Gln?Leu?Leu?Val?Ser?Glu?Thr?Ser?Cys?Gln
530?????????????????535?????????????????540
Val?Ser?Asn?Arg?Ala?Met?Lys?Gly?Leu?Thr?Thr?His?Gln?Tyr?Asp?Thr
545?????????????????550?????????????????555?????????????????560
Ser?Leu?Leu
<210>28
<211>3226
<212>DNA
＜213〉homo sapiens (Homo sapiens)
<400>28
gggagggcag?actctgggcg?ccactcccgg?gccggtcatg?aacgggccgg?cggacggcga?????60
agtggactac?aaaaaaaaat?accggaatct?gaagcggaag?ctcaagttcc?tcatctacga????120
gcacgagtgc?ttccaggagg?agctgaggaa?agcgcaaagg?aaattactga?aggtgtcccg????180
ggacaagagt?ttcctcctag?accgacttct?gcagtacgag?aacgtggatg?aagactcttc????240
ggactcagat?gccactgcat?catcagataa?cagcgagacg?gaggggacac?ccaagttgtc????300
tgacacaccg?gcccctaaga?ggaagagaag?ccctccgctg?gggggcgccc?cctctccctc????360
cagcctctcc?ctgcctcctt?caacagggtt?tccccttcag?gcctccgggg?tcccctcccc????420
atacctgagc?tcggaacggg?gccaggcctg?actgaggaga?gcaaagccca?ggacagcatc????480
ctgctgctgc?ccggcccttc?tgtgctcggg?agccccggga?gccgcactca?tcaccatctc????540
ttttgttttt?ccatcctcct?tctgtttgca?tttcttcccc?caccctcacc?ccggtccatt????600
ccgcctccca?tctccatccc?cgctcccgcc?cgcagctggc?ctcctcccgc?taccccccat????660
tcccttctga?ctacctggcc?ctgcagctgc?ccgagcccag?tcccctgagg?cccaagcggg????720
agaaacggcc?ccgcctgccc?cggaaactca?aggtaccctg?acgtgggggt?gctagggagg????780
ggcaggacgg?caggaggaca?gtcacctgag?ggaggctgaa?ggcgggggcc?tgtcagagaa????840
ccatgaacag?ttaatttggg?gacccagtca?gcacttagca?ctgaggggtg?gatcacagaa????900
gtctcggctt?acaggcttgt?atggagacaa?tcagggcaga?accagccttg?gaggacccgg????960
tgtgcagatg?ccagggatcc?cgccctttca?tggctgcagt?gtagcggggc?caggctcatt???1020
ttcccagggg?ggcgtgttgt?gtgatgccag?gtagccccag?accgggtcag?gattatggga???1080
tctagagtca?agtcaagact?gcctgttaga?aaccacgtgg?ccttgagcag?gtctctgcta???1140
ccctctaggc?ctcctaggct?tggcacctat?agggcgagtg?gtcctactcg?atgtgaccag???1200
caagcgcccc?tggagcgcct?cctgttacac?ggcagtgcac?aaagcagacc?agtgcctgcc???1260
ctcacagagc?tcacagtcta?gcaagatagg?cagaaaacac?accagaaaag?taggtacttg???1320
tggagaagaa?cagagcgagg?tccagagcat?ggggacgcca?gggggacgag?gctgacactt???1380
agggcccagc?agtctgggat?gttcttgatc?agagacttac?aggaaatggc?agcaagctgt???1440
gtggctcctg?ggcagtgttt?ctgtcgggca?gagtctgtgt?ttgggagaaa?agtaagcagg???1500
gaattgggtc?ccggagcagg?tcagaggggg?agctcatgag?gtgccagtgt?gcaggattcg???1560
ctgtgaatga?agccttggag?acagcagttc?cctggcgggg?cgcaggttgc?aggtgtggag???1620
ggggagtgat?ctaattgcat?cttaacaaaa?ttgcagacac?tggtggtcag?agaacagact???1680
gagcggtgga?gtgagttggg?acgggagtga?ggaggccacc?acggtaaacc?aggccaaggg???1740
cagagggtcc?agagggagct?gcggacccat?ttcagagaat?gagcatggga?gttcctggag???1800
gacctggtgt?gggctgtgcg?ggagagaggc?caggatgcct?ccagggcttt?agtctgagca???1860
cgagtggagt?ctccctccct?ggagatggga?gggctgtggg?accacaggct?tggaggaaaa???1920
ctgagtctgg?ccgcggatgt?gtcagatgcc?atctggaaag?gcagctagat?agcggcccgt???1980
gaggggctgc?gcatttggaa?gccgccaggc?tggcggtaga?tgcaagcctt?gggagagatg???2040
gggatgggct?gaggggcaga?gcggagtggg?gaccaactgg?gcggctccca?gggttctgga???2100
gccagaagca?gaatgagagg?ccgcatttgc?ggtggtctgg?agtacaggat?ctggagctct???2160
aatcccaact?ctgccgtttt?ctccgtgtga?ccttggccct?gtgaggcttc?acctcttgag???2220
cctgagaagc?agaggggagc?gggagtccct?gaagggccag?gggagtgagg?agatggaagg???2280
aagggagtcc?aagcagagga?aggatagctg?tgaagacaga?agcagtggcg?gcattgtgcg???2340
gagaatgtga?gtgaagggat?gaaaatcgct?cggcccgtgc?ctggccccta?gtctgtgttt???2400
tagagcgcgc?gctctggctg?ccgtgcggag?gagctggatc?tgggagaggc?tgcgggcagg???2460
gagtccagtt?aggaggctgg?gaccgcagcc?cagatggcgg?tgggaccccc?cgactgccct???2520
gtgggagggc?cgctgacctt?ccctggccgg?ggttctgggg?ctggggtcgg?acaaaaccct???2580
taaccccctt?ccacccccta?agatgccccc?ccccacgatc?ctgagcacgg?tccctcggca???2640
gatgttcagc?gatgcaggta?gcggggacga?tgccttggat?ggagacgatg?acctggtgat???2700
cgacatcccg?gagtgaccgt?gacatcacgc?catgcccacc?acggccccgc?ccggcgccct???2760
ccccgtgcca?gcacacacga?gtccagcttc?ctcggaggtg?tttattgatg?cccagctgcc???2820
atgctccggc?cactgacaca?accagaaaag?gcgtaaacat?gcacgggtgt?cccccaggag???2880
ggtggcaggg?gccctgcctt?caaaccccgg?ccccctccag?gggacagtta?tttaaacgag???2940
tggccgggag?catctgccac?ctgctgggga?ggcagagacc?ctgcaatggc?cacctcttta???3000
aaagggcagc?tgtacagggc?taggtttttt?caatgaagtt?tctgtattaa?aggagtggct???3060
ctggataaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa???3120
aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa???3180
aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaa??????????????????3226
<210>29
<211>3226
<212>DNA
＜213〉homo sapiens (Homo sapiens)
<220>
<221>CDS
<222>(38)..(448)
<223>
<400>29
gggagggcag?actctgggcg?ccactcccgg?gccggtc?atg?aac?ggg?ccg?gcg?gac??????55
Met?Asn?Gly?Pro?Ala?Asp
1???????????????5
ggc?gaa?gtg?gac?tac?aaa?aaa?aaa?tac?cgg?aat?ctg?aag?cgg?aag?ctc??????103
Gly?Glu?Val?Asp?Tyr?Lys?Lys?Lys?Tyr?Arg?Asn?Leu?Lys?Arg?Lys?Leu
10??????????????????15??????????????????20
aag?ttc?ctc?atc?tac?gag?cac?gag?tgc?ttc?cag?gag?gag?ctg?agg?aaa??????151
Lys?Phe?Leu?Ile?Tyr?Glu?His?Glu?Cys?Phe?Gln?Glu?Glu?Leu?Arg?Lys
25??????????????????30??????????????????35
gcg?caa?agg?aaa?tta?ctg?aag?gtg?tcc?cgg?gac?aag?agt?ttc?ctc?cta??????199
Ala?Gln?Arg?Lys?Leu?Leu?Lys?Val?Ser?Arg?Asp?Lys?Ser?Phe?Leu?Leu
40??????????????????45??????????????????50
gac?cga?ctt?ctg?cag?tac?gag?aac?gtg?gat?gaa?gac?tct?tcg?gac?tca??????247
Asp?Arg?Leu?Leu?Gln?Tyr?Glu?Asn?Val?Asp?Glu?Asp?Ser?Ser?Asp?Ser
55??????????????????60??????????????????65??????????????????70
gat?gcc?act?gca?tca?tca?gat?aac?agc?gag?acg?gag?ggg?aca?ccc?aag??????295
Asp?Ala?Thr?Ala?Ser?Ser?Asp?Asn?Ser?Glu?Thr?Glu?Gly?Thr?Pro?Lys
75??????????????????80??????????????????85
ttg?tct?gac?aca?ccg?gcc?cct?aag?agg?aag?aga?agc?cct?ccg?ctg?ggg??????343
Leu?Ser?Asp?Thr?Pro?Ala?Pro?Lys?Arg?Lys?Arg?Ser?Pro?Pro?Leu?Gly
90??????????????????95??????????????????100
ggc?gcc?ccc?tct?ccc?tcc?agc?ctc?tcc?ctg?cct?cct?tca?aca?ggg?ttt??????391
Gly?Ala?Pro?Ser?Pro?Ser?Ser?Leu?Ser?Leu?Pro?Pro?Ser?Thr?Gly?Phe
105?????????????????110?????????????????115
ccc?ctt?cag?gcc?tcc?ggg?gtc?ccc?tcc?cca?tac?ctg?agc?tcg?gaa?cgg??????439
Pro?Leu?Gln?Ala?Ser?Gly?Val?Pro?Ser?Pro?Tyr?Leu?Ser?Ser?Glu?Arg
120?????????????????125?????????????????130
ggc?cag?gcc?tgactgagga?gagcaaagcc?caggacagca?tcctgctgct??????????????488
Gly?Gln?Ala
135
gcccggccct?tctgtgctcg?ggagccccgg?gagccgcact?catcaccatc?tcttttgttt????548
ttccatcctc?cttctgtttg?catttcttcc?cccaccctca?ccccggtcca?ttccgcctcc????608
catctccatc?cccgctcccg?cccgcagctg?gcctcctccc?gctacccccc?attcccttct????668
gactacctgg?ccctgcagct?gcccgagccc?agtcccctga?ggcccaagcg?ggagaaacgg????728
ccccgcctgc?cccggaaact?caaggtaccc?tgacgtgggg?gtgctaggga?ggggcaggac????788
ggcaggagga?cagtcacctg?agggaggctg?aaggcggggg?cctgtcagag?aaccatgaac????848
agttaatttg?gggacccagt?cagcacttag?cactgagggg?tggatcacag?aagtctcggc????908
ttacaggctt?gtatggagac?aatcagggca?gaaccagcct?tggaggaccc?ggtgtgcaga????968
tgccagggat?cccgcccttt?catggctgca?gtgtagcggg?gccaggctca?ttttcccagg???1028
ggggcgtgtt?gtgtgatgcc?aggtagcccc?agaccgggtc?aggattatgg?gatctagagt???1088
caagtcaaga?ctgcctgtta?gaaaccacgt?ggccttgagc?aggtctctgc?taccctctag???1148
gcctcctagg?cttggcacct?atagggcgag?tggtcctact?cgatgtgacc?agcaagcgcc???1208
cctggagcgc?ctcctgttac?acggcagtgc?acaaagcaga?ccagtgcctg?ccctcacaga???1268
gctcacagtc?tagcaagata?ggcagaaaac?acaccagaaa?agtaggtact?tgtggagaag???1328
aacagagcga?ggtccagagc?atggggacgc?cagggggacg?aggctgacac?ttagggccca???1388
gcagtctggg?atgttcttga?tcagagactt?acaggaaatg?gcagcaagct?gtgtggctcc???1448
tgggcagtgt?ttctgtcggg?cagagtctgt?gtttgggaga?aaagtaagca?gggaattggg???1508
tcccggagca?ggtcagaggg?ggagctcatg?aggtgccagt?gtgcaggatt?cgctgtgaat???1568
gaagccttgg?agacagcagt?tccctggcgg?ggcgcaggtt?gcaggtgtgg?agggggagtg???1628
atctaattgc?atcttaacaa?aattgcagac?actggtggtc?agagaacaga?ctgagcggtg???1688
gagtgagttg?ggacgggagt?gaggaggcca?ccacggtaaa?ccaggccaag?ggcagagggt???1748
ccagagggag?ctgcggaccc?atttcagaga?atgagcatgg?gagttcctgg?aggacctggt???1808
gtgggctgtg?cgggagagag?gccaggatgc?ctccagggct?ttagtctgag?cacgagtgga???1868
gtctccctcc?ctggagatgg?gagggctgtg?ggaccacagg?cttggaggaa?aactgagtct???1928
ggccgcggat?gtgtcagatg?ccatctggaa?aggcagctag?atagcggccc?gtgaggggct???1988
gcgcatttgg?aagccgccag?gctggcggta?gatgcaagcc?ttgggagaga?tggggatggg???2048
ctgaggggca?gagcggagtg?gggaccaact?gggcggctcc?cagggttctg?gagccagaag???2108
cagaatgaga?ggccgcattt?gcggtggtct?ggagtacagg?atctggagct?ctaatcccaa???2168
ctctgccgtt?ttctccgtgt?gaccttggcc?ctgtgaggct?tcacctcttg?agcctgagaa???2228
gcagagggga?gcgggagtcc?ctgaagggcc?aggggagtga?ggagatggaa?ggaagggagt???2288
ccaagcagag?gaaggatagc?tgtgaagaca?gaagcagtgg?cggcattgtg?cggagaatgt???2348
gagtgaaggg?atgaaaatcg?ctcggcccgt?gcctggcccc?tagtctgtgt?tttagagcgc???2408
gcgctctggc?tgccgtgcgg?aggagctgga?tctgggagag?gctgcgggca?gggagtccag???2468
ttaggaggct?gggaccgcag?cccagatggc?ggtgggaccc?cccgactgcc?ctgtgggagg???2528
gccgctgacc?ttccctggcc?ggggttctgg?ggctggggtc?ggacaaaacc?cttaaccccc???2588
ttccaccccc?taagatgccc?ccccccacga?tcctgagcac?ggtccctcgg?cagatgttca???2648
gcgatgcagg?tagcggggac?gatgccttgg?atggagacga?tgacctggtg?atcgacatcc???2708
cggagtgacc?gtgacatcac?gccatgccca?ccacggcccc?gcccggcgcc?ctccccgtgc???2768
cagcacacac?gagtccagct?tcctcggagg?tgtttattga?tgcccagctg?ccatgctccg???2828
gccactgaca?caaccagaaa?aggcgtaaac?atgcacgggt?gtcccccagg?agggtggcag???2888
gggccctgcc?ttcaaacccc?ggccccctcc?aggggacagt?tatttaaacg?agtggccggg???2948
agcatctgcc?acctgctggg?gaggcagaga?ccctgcaatg?gccacctctt?taaaagggca???3008
gctgtacagg?gctaggtttt?ttcaatgaag?tttctgtatt?aaaggagtgg?ctctggataa???3068
aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa???3128
aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa???3188
aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaa???????????????????????????3226
<210>30
<211>137
<212>?PRT
＜213〉homo sapiens (Homo sapiens)
<400>30
Met?Asn?Gly?Pro?Ala?Asp?Gly?Glu?Val?Asp?Tyr?Lys?Lys?Lys?Tyr?Arg
1???????????????5???????????????????10??????????????????15
Asn?Leu?Lys?Arg?Lys?Leu?Lys?Phe?Leu?Ile?Tyr?Glu?His?Glu?Cys?Phe
20??????????????????25??????????????????30
Gln?Glu?Glu?Leu?Arg?Lys?Ala?Gln?Arg?Lys?Leu?Leu?Lys?Val?Ser?Arg
35??????????????????40??????????????????45
Asp?Lys?Ser?Phe?Leu?Leu?Asp?Arg?Leu?Leu?Gln?Tyr?Glu?Asn?Val?Asp
50??????????????????55??????????????????60
Glu?Asp?Ser?Ser?Asp?Ser?Asp?Ala?Thr?Ala?Ser?Ser?Asp?Asn?Ser?Glu
65??????????????????70??????????????????75??????????????????80
Thr?Glu?Gly?Thr?Pro?Lys?Leu?Ser?Asp?Thr?Pro?Ala?Pro?Lys?Arg?Lys
85??????????????????90??????????????????95
Arg?Ser?Pro?Pro?Leu?Gly?Gly?Ala?Pro?Ser?Pro?Ser?Ser?Leu?Ser?Leu
100?????????????????105?????????????????110
Pro?Pro?Ser?Thr?Gly?Phe?Pro?Leu?Gln?Ala?Ser?Gly?Val?Pro?Ser?Pro
115?????????????????120?????????????????125
Tyr?Leu?Ser?Ser?Glu?Arg?Gly?Gln?Ala
130?????????????????135
<210>31
<211>18
<212>DNA
＜213〉primer
<400>31
ggcacctcag?caaccagt????????????????????????????????????????????????18
<210>32
<211>19
<212>DNA
＜213〉primer
<400>32
cgaaactaac?taaacccga???????????????????????????????????????????????19
<210>33
<211>21
<212>DNA
＜213〉primer
<400>33
cagtcttcgc?tcactacagc?c????????????????????????????????????????????21
<210>34
<211>20
<212>DNA
＜213〉primer
<400>34
ccaatccaaa?atgaccccgt??????????????????????????????????????????????20
<210>35
<211>21
<212>DNA
＜213〉primer
<400>35
aagatgaatc?cgttgatctc?g????????????????????????????????????????????21
<210>36
<211>20
<212>DNA
＜213〉primer
<400>36
tcgtttgaca?ggtcttccct??????????????????????????????????????????????20
<210>37
<211>20
<212>DNA
＜213〉primer
<400>37
aagccctaca?gtgcagagga??????????????????????????????????????????????20
<210>38
<211>20
<212>DNA
＜213〉primer
<400>38
gagaaccaca?aagtacgacg???????????????????????????????????????????????20
<210>39
<211>21
<212>DNA
＜213〉primer
<400>39
gaagaggagg?aggaagagga?g?????????????????????????????????????????????21
<210>40
<211>20
<212>DNA
＜213〉primer
<400>40
gtggaaggga?cactttgttc???????????????????????????????????????????????20
<210>41
<211>18
<212>DNA
＜213〉primer
<400>41
ctttcagctc?ggctgctc?????????????????????????????????????????????????18
<210>42
<211>21
<212>DNA
＜213〉primer
<400>42
gtacctcata?tcgcagtagg?g?????????????????????????????????????????????21
<210>43
<211>18
<212>DNA
＜213〉primer
<400>43
gagggtgcgg?aggacaac?????????????????????????????????????????????????18
<210>44
<211>20
<212>DNA
＜213〉primer
<400>44
caaggacggt?gacatgaggt???????????????????????????????????????????????20
<210>45
<211>18
<212>DNA
＜213〉primer
<400>45
cttgttcctg?ccgctttc?????????????????????????????????????????????????18
<210>46
<211>22
<212>DNA
＜213〉primer
<400>46
tgtctactac?tgacgtttta?ct?????????????????????????????????????????????22
<210>47
<211>19
<212>DNA
＜213〉primer
<400>47
atcgtcggcc?agtttatcc?????????????????????????????????????????????????19
<210>48
<211>20
<212>DNA
＜213〉primer
<400>48
aatggggaac?gacaagtctc????????????????????????????????????????????????20
<210>49
<211>18
<212>DNA
＜213〉primer
<400>49
gggagggcag?actctggg??????????????????????????????????????????????????18
<210>50
<211>18
<212>DNA
＜213〉primer
<400>50
agtgcggtac?gggtggtg??????????????????????????????????????????????????18
Claims (10)
1. isolating people's albumen with cancer suppressing function is characterized in that, it comprises the polypeptide of the aminoacid sequence with the group of being selected from down: SEQ ID NO:3,6,9,12,15,18,21,24,27,30;
Or its conservative property variation polypeptide or its active fragments or its reactive derivative.
2. polypeptide as claimed in claim 1 is characterized in that, this polypeptide is the polypeptide with aminoacid sequence of the group of being selected from down: SEQ ID NO:3,6,9,12,15,18,21,24,27,30.
3. isolating polynucleotide is characterized in that, it comprises a nucleotide sequence, and this nucleotide sequence is shown at least 85% homogeny with a kind of nucleotides sequence that is selected from down group:
(a) coding is as the polynucleotide of polypeptide as described in claim 1 and 2;
(b) with polynucleotide (a) complementary polynucleotide.
4. polynucleotide as claimed in claim 3 is characterized in that, the polypeptide of this polynucleotide encoding has the aminoacid sequence of the group of being selected from down: SEQ ID NO:3,6,9,12,15,18,21,24,27,30.
5. polynucleotide as claimed in claim 3 is characterized in that, the sequence of these polynucleotide is selected from down group:
SEQ ID NO:2,5,8,11,14,17,20,23,26,29 coding region sequence or full length sequence.
6. a carrier is characterized in that, it contains the described polynucleotide of claim 3.
7. a genetically engineered host cell is characterized in that, it is a kind of host cell that is selected from down group:
(a) host cell that transforms or transduce with the described carrier of claim 6;
(b) host cell that transforms or transduce with the described polynucleotide of claim 3.
8. the preparation method of the polypeptide of the people's protein-active with cancer suppressing function is characterized in that this method comprises:
(a) have under the proteic condition of people of cancer suppressing function suitable the expression, cultivate the described host cell of claim 7;
(b) from culture, isolate the polypeptide of people's protein-active with cancer suppressing function.
9. energy and the described people's protein-specific bonded antibody of claim 1 with cancer suppressing function.
10. a pharmaceutical composition is characterized in that, it contains the described polypeptide of claim 1 and the pharmaceutically acceptable carrier of safe and effective amount.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021454353A CN1222616C (en) | 2002-11-20 | 2002-11-20 | Novel human protein with cancer-inhibiting function and coding sequence thereof |
| PCT/CN2003/000639 WO2004014946A1 (en) | 2002-08-07 | 2003-08-07 | A novel homo protein with cancer suppressing function and its coding sequence |
| AU2003255098A AU2003255098A1 (en) | 2002-08-07 | 2003-08-07 | A novel homo protein with cancer suppressing function and its coding sequence |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021454353A CN1222616C (en) | 2002-11-20 | 2002-11-20 | Novel human protein with cancer-inhibiting function and coding sequence thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1502626A true CN1502626A (en) | 2004-06-09 |
| CN1222616C CN1222616C (en) | 2005-10-12 |
Family
ID=34232437
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021454353A Expired - Fee Related CN1222616C (en) | 2002-08-07 | 2002-11-20 | Novel human protein with cancer-inhibiting function and coding sequence thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110913897A (en) * | 2017-06-22 | 2020-03-24 | 古斯达威罗斯研究所 | Human endogenous retroviral proteins |
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2002
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110913897A (en) * | 2017-06-22 | 2020-03-24 | 古斯达威罗斯研究所 | Human endogenous retroviral proteins |
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| CN1222616C (en) | 2005-10-12 |
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