CN1493697A - Pesticide residue enzymatic detection method using same kind deactivated enzyme as reference and its device - Google Patents
Pesticide residue enzymatic detection method using same kind deactivated enzyme as reference and its device Download PDFInfo
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- CN1493697A CN1493697A CNA021376832A CN02137683A CN1493697A CN 1493697 A CN1493697 A CN 1493697A CN A021376832 A CNA021376832 A CN A021376832A CN 02137683 A CN02137683 A CN 02137683A CN 1493697 A CN1493697 A CN 1493697A
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Abstract
A process for detecting the residual agricultural chemicals by using the deactivated same enzyme as reference includes making the color comparison utensil holder in constant-temp state, loading the specimen respectively in reaction chambers and reference chambers, which contain respectively the active enzyme, deactive enzyme, substrate and coloring liquid, stirring, light irradiation, receiving optical signals, and signal processing to detecte the residual agricultural chemicals. Its equipment is composed of light source, constant-temp color comparison utensil holder including reaction chambers, reference chamber and storage, photosensing tube and signal processing device. Its advantages are low cost and fast detection.
Description
Technical field
The present invention relates to a kind of pesticide residue enzymatic detection method and device thereof of making reference with same kind deactivated enzyme.
Background technology
Organophosphorus pesticide is the agricultural chemicals that generally uses at present, the organophosphorus pesticide property accumulated in animal body is little, have the fast and residual low characteristics of degraded, but organophosphorus pesticide residual quantity in vegetable food product is higher, especially the easiest absorption organophosphorus of fruits and vegetables, has stronger acute toxicity, general provision some days planted agents before results forbid using, yet illegal orchard worker, vegetable grower are the major causes that produce acute toxicity when causing the human consumer edible at vegetables, a large amount of spraying pesticides of fruit before sales.Prevent the agricultural-food listing that pesticide residue exceeds standard, forbid exceed standard one of the important means of sale of agricultural-food of agricultural chemicals to be exactly frequent, form institutionalization the listing agricultural-food are detected.What the detection pesticide residue was the most frequently used at present is vapor-phase chromatography and high performance liquid chromatography, needs several days time.In this conventional detection method exist test procedure numerous and diverse, waste time and energy, need the professional to operate, detect shortcomings such as cost height, can not be used for on-the-spot the detection and rapid detection.In recent years, though relevant in the world paper publishing is not seen to have to supply the method and apparatus of enforcement.
Summary of the invention
Purpose of the present invention is for the residual quantity of rapid detection organophosphorus pesticide in vegetable food product, provides to make the pesticide residue enzymatic detection method and the device thereof of reference with same kind deactivated enzyme for what implement.
Enzyme can catalysis its substrate decompose, hydrolysate may itself have color, or colourless hydrolysate and certain developer generation coloring matter that reacts, how much can the judging by the depth of color of hydrolysate.The depth of judging color changes and can realize with the reference method.Enzymic catalytic reaction is very responsive to temperature, answers control reaction temperature during reaction, utilizes the reference method and reaction solution is carried out thermostatically controlled Detecting Pesticide method the steps include:
1, makes thermostatical color comparison ware frame reach temperature constant state, will move into the constant temperature storage tank, make it reach temperature constant state with base fluid, demonstration liquid and the damping fluid that substrate is equipped with;
2, the solution of testing sample after being processed into processing such as juice, filtration places the reaction chamber that is injected with enzyme alive, substrate and colour developing liquid respectively and is injected with the reference chamber of deactivated enzyme, substrate and colour developing liquid;
3, stir;
4, by reaction chamber and reference chamber irradiation in the light source while contrasting colour ware of single wavelength;
5, the optical signals photosensitive tube through reaction chamber and the transmission of reference chamber institute receives, and signal relatively detects the residual quantity of agricultural chemicals through amplifying, handling according to example reaction chamber that includes enzyme alive and the absorbancy that includes the reference chamber of fermentoid.
For realizing that the device that aforesaid method designs comprises: light source, thermostatical color comparison ware frame, fan, photosensitive tube and the signal processor unit formed by reaction chamber, reference chamber, storage tank and semiconductor cooler.Signal processor unit is made up of amplifier, A/D converter, micro-chip, printer, indicating meter, keyboard, classification combined aural and visual alarm.Signal processor unit is classification combined aural and visual alarm that is connected and is formed by differentiating amplifier, comparer and acousto-optic generator also.
The advantage of present method and device is: the detection step is simple, time saving and energy saving, the detection cost is low, available field quick detection.
Description of drawings
Fig. 1 is for making the pesticide residue enzymatic proofing unit schematic diagram of reference with same kind deactivated enzyme;
Fig. 2 is a classification combined aural and visual alarm schematic diagram
Fig. 3 is reaction chamber/reference chamber structural representation;
Fig. 4 is a cuvette radiator valve structural representation.
Embodiment
The device that present method adopted is by shown in Fig. 1,2, comprise light source 1, by thermostatical color comparison ware frame 16, fan 4, photosensitive tube 5 and signal processor unit that reaction chamber 2, reference chamber 3, storage tank 13,14,15 are formed, signal processor unit is to be made up of amplifier 6, A/D converter 7, micro-chip 8, printer 9, indicating meter 10, keyboard 11, classification combined aural and visual alarm 12.Signal processor unit also can be a classification combined aural and visual alarm that is connected by differentiating amplifier 17, comparer 18 and the acousto-optic generator of being made up of rattler 20 and photodiode 21 and forms.Light source 1 is formed double light path by photodiode, and the two-way light of identical wavelength is simultaneously by reaction chamber 2 and reference chamber 3.By shown in Figure 3, be equipped with miniature magnetic agitator 22 below reaction chamber 2/ reference chamber 3, stirrer 23 places reaction chamber 2/ reference chamber 3, plays the purpose to liquid agitation in reaction chamber/reference chamber cuvette, and churning time is by keyboard to set up.Cell rack reaches constant temperature when detecting for making, the cell rack of design as shown in Figure 4, it comprises thermoelectric refrigeration/well heater 25, radiator element 26, opticglass barrier sheet 27, lagging material 28, base plate 29 and temperature sensor 30.The cuvette internal frame of being made by the high material of thermal conducting rate such as aluminium alloy, stainless steel 24 directly contacts with thermoelectric refrigeration/well heater 25, and temperature sensor 30 places the bottom of internal frame.The principle of work of thermoelectric refrigeration/well heater is based on Peltier effect: during loop that the electro-conductive material that constitutes by two kinds of different electro-conductive materials when direct current constitutes, will produce the neither endothermic nor exothermic phenomenon on the contact.When temperature was higher than design temperature, internal frame needed cooling, and at this moment, semiconductor cooler should be operated in refrigerating state, and the end face that contacts with internal frame is a cold junction, and the end face that contacts with scatterer is the hot junction.And when the internal frame temperature is lower than design temperature, needing heating, the end face that contact with internal frame this moment is the hot junction, and the end face that contacts with scatterer is a cold junction.Change the sense of current that flows through TEC, just can realize the thermal conversion of freezing/add.
Method of the present invention realizes by said apparatus: at first make cell rack reach temperature constant state, the solution of testing sample after breaking into processing such as juice, filtration places the reaction chamber 2 that is injected with enzyme alive, substrate and colour developing liquid respectively and is injected with the reference chamber 3 of deactivated enzyme, substrate and colour developing liquid; 20 short period of time of miniature magnetic agitator that are equipped with below by reaction chamber 2/ reference chamber 3 stir, as 5 seconds; By reaction chamber 2 and reference chamber 3 irradiations in 1 while of the light source contrasting colour ware of single wavelength; Optical signals photosensitive tube 5 through reaction chamber 2 and 3 transmissions of reference chamber receives, detected signal amplifies the back to signal processing means processes, the present invention has designed two kinds of processing schemes to detected result: a kind of is as shown in Figure 1, photosignal shows on indicating meter 9 and by mini-printer 8 printouts after micro-chip 7 is handled; And report to the police by classification sound and light alarm 11 simultaneously.The another kind micro-chip does not have indicating meter and printout.As shown in Figure 2, the output signal of two photo-sensors 5 links to each other with differentiating amplifier 17, and the output of differentiating amplifier links to each other with three comparers 18, and the other end 19 of comparer is different standard input.According to detected result, put azarin, Huang, green three photodiodes 21 respectively, with the prompting agricultural chemicals degree that whether exceeds standard and exceed standard, also activate 20, three rattlers of corresponding rattler simultaneously and have different audio frequency or point out with language " severe overweight, exceed standard, qualified ".Like this, according to the absorbancy comparison of example reaction chamber that includes enzyme alive and the reference chamber that includes fermentoid, detect the residual quantity of agricultural chemicals.Used enzyme can adopt enzyme powder, enzyme sheet or solidify enzyme in reaction chamber/reference chamber, and the source of enzyme is Pseudocholinesterase, plant enzyme or organophosphor hydrolytic enzyme.
Claims (7)
1, a kind ofly makes the pesticide residue enzymatic detection method of reference, be characterised in that, the steps include: with same kind deactivated enzyme
(1) make cell rack reach temperature constant state
(2) the testing sample economy-combat becomes solution after the processing such as fruit juice, filtration to place the reaction chamber that is injected with enzyme alive, substrate and colour developing liquid respectively and is injected with the reference chamber of deactivated enzyme, substrate and colour developing liquid;
(3) stir;
(3) by reaction chamber and reference chamber irradiation in the light source while contrasting colour ware of single wavelength;
(4) the optical signals photosensitive tube through reaction chamber and the transmission of reference chamber institute receives, and signal relatively detects the residual quantity of agricultural chemicals through amplifying, handling according to example reaction chamber that includes enzyme alive and the absorbancy that includes the reference chamber of fermentoid.
2, a kind of device that provides for aforesaid method, it is characterized in that, comprising: light source (1), thermostatical color comparison ware frame (16), fan (4), photosensitive tube (5) and the signal processor unit formed by reaction chamber (2), reference chamber (3), storage tank (13), (14), (15).
3, device according to claim 2, it is characterized in that described signal processor unit is connected to form by amplifier (6), A/D converter (7), micro-chip (8), printer (9), indicating meter (10), keyboard (11), classification combined aural and visual alarm (12).
4, device according to claim 2, it is characterized in that described signal processor unit also can be the classification combined aural and visual alarm that is connected and is formed by differentiating amplifier (17), comparer (18) and the acousto-optic generator of being made up of rattler (20) and photodiode (21).
5, device according to claim 2 is characterized in that, is equipped with miniature magnetic agitator (22) below described reaction chamber (2)/reference chamber (1).
6, device according to claim 2, it is characterized in that, described cell rack comprises thermoelectric refrigeration/well heater (25), radiator element (26), opticglass barrier sheet (27), lagging material (28), base plate (29) and temperature sensor (30 in cell rack, cuvette internal frame (24) directly contacts with thermoelectric refrigeration/well heater (25), and temperature sensor (30) places the bottom of internal frame.
7, device according to claim 2 is characterized in that, described light source (1) is formed double light path by photodiode, and the two-way light of identical wavelength is simultaneously by reaction chamber (2) and reference chamber (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021376832A CN100500862C (en) | 2002-10-29 | 2002-10-29 | Pesticide residue enzymatic detection method using same kind deactivated enzyme as reference and its device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021376832A CN100500862C (en) | 2002-10-29 | 2002-10-29 | Pesticide residue enzymatic detection method using same kind deactivated enzyme as reference and its device |
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| Publication Number | Publication Date |
|---|---|
| CN1493697A true CN1493697A (en) | 2004-05-05 |
| CN100500862C CN100500862C (en) | 2009-06-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021376832A Expired - Fee Related CN100500862C (en) | 2002-10-29 | 2002-10-29 | Pesticide residue enzymatic detection method using same kind deactivated enzyme as reference and its device |
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| CN (1) | CN100500862C (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1908622B (en) * | 2005-08-05 | 2010-07-07 | 五鼎生物技术股份有限公司 | Analysis method of enzyme inhibiting agent and module thereof |
| CN105319212A (en) * | 2015-11-13 | 2016-02-10 | 无锡艾科瑞思产品设计与研究有限公司 | Automatic pesticide residue detector |
| CN106706865A (en) * | 2016-11-18 | 2017-05-24 | 百奥森（江苏）食品安全科技有限公司 | Novel rapid detection card of plant enzyme source pesticide residues |
-
2002
- 2002-10-29 CN CNB021376832A patent/CN100500862C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1908622B (en) * | 2005-08-05 | 2010-07-07 | 五鼎生物技术股份有限公司 | Analysis method of enzyme inhibiting agent and module thereof |
| CN105319212A (en) * | 2015-11-13 | 2016-02-10 | 无锡艾科瑞思产品设计与研究有限公司 | Automatic pesticide residue detector |
| CN106706865A (en) * | 2016-11-18 | 2017-05-24 | 百奥森（江苏）食品安全科技有限公司 | Novel rapid detection card of plant enzyme source pesticide residues |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100500862C (en) | 2009-06-17 |
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Granted publication date: 20090617 Termination date: 20111029 |