CN1492758A

CN1492758A - Method and formula for anti-tumor and anti-matastatic effect

Info

Publication number: CN1492758A
Application number: CNA018229263A
Authority: CN
Inventors: C��ֶ�; 布赖恩·C·贾尔斯
Original assignee: Individual
Current assignee: Individual
Priority date: 2001-02-28
Filing date: 2001-02-28
Publication date: 2004-04-28
Also published as: NO20033797D0; JP2004530659A; EP1372631A1; MXPA03007783A; EP1372631A4; WO2002069955A1; NO20033797L; CA2442396A1; US20050260277A1

Abstract

The invention discloses an expeditious method and formula for treating cancer patients that reaulst in tumor suppression and remission, the suppression of mobilization and adhesion of cancer cells, and repression of pathogens, such as infectious bacteria and viruses. Administration is by oral ingestion or direct injection into tumors or intravenous drip etc. The invention employs control of intracellular and extracelular ionic physiology by administering alkaline salts, thereby restoring localize and/or systemic cellular ionic physiology, depriving cancerous cells of their ability to grow rapidly, and simultaneously normalizing their local environment, thereby inhibiting angiogenesis, enabling immune responses, and simultaneously reducing pain. The method further promotes correction of acidotic conditions often associated with cancer and other diseases, thereby potentiating biological functions of immunity and repair.

Description

The method and formulation of antitumor and metastasis effect

Technical field

The present invention relates generally to pharmacology and drug world, and more specific be specifically related to be used for will the treatment effective dose therapeutic agent deliver medicine to method and formulation in cancer patient's the body, the activity of wherein said therapeutic agent matter by changing whole body, part and/or cell plasma physiology and have metastasis, disappear and suppress pernicious and non-malignant tumors and anti-adhesive and anti-mobilization.For the recurrence of prophylaxis of cancer and regrowing of tumor, provide a large amount of pain relief means simultaneously.

Background technology

Cancer cell is different with normal cell in some respects.In fact the aspect that all cancer cell are different from the normal health cell is that most of energy of cancerous cell all is obtained from glycolysis.And the oxidative metabolism that the normal health cell uses has only the sub-fraction energy from glycolysis.Only in the situation of exception, for example normal cell just can obtain its most energy from glycolysis in extreme muscle strength outburst process, but under normal condition, they use oxidative metabolism.Therefore, energy metabolism is the generality difference between normal cell and cancerous cell.

One of metabolic feature of cancerous cell incorrect energy is that they can produce acid, as lactic acid or the like.This can make near the pH value cancerous cell (pHe that compares with general pH as whole biosystem pH, or the pH of outside) well below normal pH (the negative logarithm of hydrogen ion activity: pH=-log (H+)).The lower pHe value that cancer showed generally can increase rapidly and more may be fatal for patients.Low pHe can be used as angiopoietic triggering agent, thereby makes the blood that flows to the tumor material increase.Low pHe has reduced the usefulness of immunne response to cancer.The metabolic energy charge of incorrect energy (ATP/ (ADP+Pi)) generally is lower than the energy charge of normal energy metabolism.Cancer cell has usually and is lower than pH (pHi, or the pH of cell interior) in the Normocellular born of the same parents.That is, it comprises more hydrion than normal cell.In addition, cancer cell generally has and is different from Normocellular cell plasma and distributes, and has excessive sodium and very excessive calcium in the normal cell usually, and the general deficiency of potassium.Cancerous cell generally has and is different from Normocellular ion flow.Cancerous cell generally has the transmembrane potential lower than Normocellular elecrtonegativity (inner with respect to the outside).But unusual ion concentration is as low pHi, intracellular high sodium or high potassium inducing apoptosis and/or cause the identification of immune system to this cancer cell.

The development of cancer relate to the growth of neoplastic cell and the destruction that causes by regulation process between balance.Follow the gene alteration that cancer takes place to cause the great interest of people, and they have been had a lot of understandings.Such change is the prerequisite that disease develops, but it also is not enough to overcome the natural defence of body.Therefore, the treatment of the natural defending system by strengthening body can prophylaxis of cancer, wherein said natural defending system such as immunne response and programmed cell death, such treatment have been changed the balance between cancer development and the cancer disappearance, and this balance is moved towards the direction that promotes cancer to disappear.

Be to find that as the key of the method for selectively targeted cancerous cell a kind of normal cell toxicity for health is very little or do not have toxicity but but make nonviable preparation of cancerous cell and Therapeutic Method with unusual energy metabolism.A kind of ideal Therapeutic Method can also reduce the acidization that is produced by the cancerous cell material, so that physiological pHe is near 7.37 to 7.40.If pHe approaches normally, then metabolic function can not be subjected to acidosic harm, and immune system has active anticancer in the biochemistry/ionic environment of the best, and can not occur in the situation that pH is reduced the promotion neovascularization that occurs when reacting.Alkalization fully also will relax the acidosis effect of neoplasm necrosis.Such treatment is obviously all very useful to many degenerative diseases relevant with acidosis.

Caesium is to have the chemistry similar to potassium and the alkali metal of physical property with rubidium, but the atomic weight of the two is higher.In fact it does not promptly exist in food and the water at biosphere.Potassium is main cation in the living cells, and potassium current pair cell ion physiology is very important.Mammalian cell generally responds to the situation by the inductive glycolysis energy metabolism of a large amount of potassium currents.The membrane flow of striding of caesium and rubidium is to be controlled by the celelular mechanism identical with the active celelular mechanism of control potassium ion with cells accumulation, but they move with lower speed and can be accumulate to different degree, therefore can change the ion physiology of cell, comprise the transmembrane movement that can suppress potassium.

The form of therapy prior art

1. operation: mainly be to rely on operation to remove this tumor load to treatment for cancer in the prior art.There are the stress relevant and the excessive risk of high cost and transfer, removed (remaining usually cancerous cell can be survived and be carried out activity) fully so be difficult to guarantee cancerous tissue, so generally need carry out other treatment with operation.Operation also needs to reduce pain with independent medicine, and this can impel chemistry or physiology to go up addiction.

2. chemotherapy: prior art is mainly come that for kinetics penetrating pharmaceutical or radiation serious degenerative disease such as cancer are treated institute by medication and is formed, do not consider the electrophysiology reaction of system's biology, therefore this treatment also can further increase the weight of acidosis situation, inhibition or destroy the physiological health that normally carries out quick fissional cell and/or harm patient.

The onset inherently of the method for these prior aries and medicine is slow and efficient is low, particularly all the more so in the situation of latter stage or terminal cancer, and serious adverse is arranged, as antigenic action, and the treatment window that provides is very narrow, the ion physiology there is adverse effect and can disturbs the activity of necessary pH balance and key enzyme and assimilation of essential nutrient substance or the like, thereby greatly reduce the probable life of metabolic function and cell and organ, can reduce its biological function and usefulness in addition.

3. radiation: the radiation of using in the prior art can cause nonvolatil infringement and also can further promote gathering of acid toxin, can produce harm to biotic environment.

4. treat relevant treatment with the ion physiology:, proposed some chemical drugss as antitumor agent based on the peculiar property of cancer energy metabolism.For example, proposed to be used as antitumor agent, but these materials only show common antitumor action with Na/H exchanger inhibitor and membranous sub-pump inhibitor.They are different from theme material of the present invention and poorer than theme material of the present invention.

Utilization has been carried out some previous works based on the treatment of caesium and rubidium.But the carrying out of this class work is unprofitable to the latest development aspect the ion result of the energy metabolism of understanding the cancerous cell uniqueness.Although early stage research has certain hope, the technology that usefulness cannot not obtain previously only may correctly be prepared and accurately give reliable therapeutic dose and treat with caesium and/or rubidium.

5. by the caused disease of microorganism: virus, antibacterial can progressively develop immunity to drugs when contact with many antibiotic with other infectious microorganism or even the generation immunity, thereby make this antibiotic become invalid at last.Microorganism usually has and can have been eliminated its probability that pharmaceutically active is developed immunity to drugs by the acidosic energy metabolism of ion physiology institute targeting.

The biotic environment of all cancer cell all has very narrow and special existence district, and this existence district is confined to narrow pH scope and oxidation-reduction potential (ORP) (Fig. 1).But, notice healthy people's cell outside the physiological environment of cancer the pH scope and ORP in have best function.By helping to remove disacidify from body fluid, this method and formulation will make these fluids carry out circulation time towards moving with aerobic corresponding to ORP of homeostatic metabolic function and pH scope when it by biosystem.Therefore, from system, eliminate remaining cancerous cell and tumor mass and other acidic materials that gather by normal metabolism discharging (kidney, breathing, digestive tract) subsequently.Such reaction can be used as the means of prevent disease recurrence and tumor regrowth.

Degenerative disease demonstrates the identical imbalance of acute maladjustment disease reaction with the biological environment of anoxia, acidity-hypoxia.Ion adjusted biology more also can ease the pain and swelling and many imbalances relevant with these diseases.Therefore, negative charge has reduced neuronic being overexcited property, to stress sexual biology inflammatory complex such as superoxides, peroxide or the like handle, thereby pHi normalization and stabilisation and contratoxin are handled.

The purpose and the advantage of invention

The present invention is intended to disclose a kind of nontoxic pharmaceutically useful medicine, it can be delivered medicine to people or other mammal that suffers from cancer, to increase pHe and pHi and to reduce general acidity, can also treat the metastatic tumour on whole body and the one or more primary tumor site with extremely low toxicity.Be noted that such extremely low toxicity is rarely found or do not exist in the metastasis of " prior art " and anticarcinogenesis derivant.The present invention has used a kind of manufacture method and preparation, it comprises the electrolyzable saline of use, this saline can produce the pharmaceutically acceptable accurately preparation that active component is had optimum utilization, and the effective treatment supplementary means of uniting use with treatment of cancer such as surgical intervention, radiation or chemotherapy or the like of various prior aries can be treated or can be used as to wherein said active component effectively separately.In addition, for this Therapeutic Method and the most responsive cancerous cell of preparation is that these have the product cell that acid metabolic is maximum, hypertrophy is fastest and to conventional therapy of the prior art such as chemotherapy and/or radiation and the most obstinate cell of operation, therefore these cancers of elimination might be to sensitivity of the present invention with other treatment.The present invention has also promoted the hydration of body fluid and has stimulated the drainage of acid toxin.Cancerous cell can not form the Drug resistance that resembles many treatments of the prior art formed to this treatment/preparation.Because the formation of neovascularity betides in the low pH reaction, so can suppress the growth of neovascularity in tumor by near the acidization that reduces or eliminates the tumor cell.Regulating dosage makes in the scope of its pHi that drops on institute's targeting and pHe, a kind of effect of controllable degree is provided, make the stabilisation of pernicious and non-malignant tumors take place, avoided the misery or the mortality rate that accompany and take place with the necrosis of tumor with disappearing and eliminating in mode predictable and progressively.The physiological change of ion has reduced the secretion activity of cancerous cell.The ability that the two has all reduced its secretion or has resisted cancer therapy drug, this is many drug-fast bases, and it has also suppressed the ability of the oncoprotein enzyme of secretion promotion neoplasm metastasis.

Describe a kind of method that can assess the curative effect of particular patient in detail, this method is to adopt the physiological observation of relevant cancer ion, wherein said cancer ion physiology such as acid generation, lactate produce, calcium gathers, sodium gathers or the like, and this observation makes predictability ground adjust and curative effect is assessed dosage.In addition, it is very suitable for first kind of selection as intervention means, its reduction greatly or eliminate pain and the cancer that can disappear, postpone expensive test and other only be used for the treatment of refractory cancers, effectively stop part and whole body the acidosis circulation, be provided for treating that many cancers are rapid-action, expense economy, effective preparation and can reduce the effective dose of active component.

And the growing environment of prevention and/or elimination cancer with the various treatment compatibilitys of the prior art and/or synergism, can be kept iuntercellular useful in the ionic environment and change.

Of the present invention open

The invention provides the metastasis effect and to the disappearing of cancer, and suppressed the ability that secretion promotes the active protease of neoplasm metastasis, and very effective in prophylaxis of cancer survival environment.It can use separately or be used in combination with operation, radiation or chemotherapy or the like, treatment means or the secular means of keeping as a kind of short-term, disclose use a kind of specific substantially Therapeutic Method and in the past not used preparation with and the effective ways making and use, it has stoped the origin of cancer forming process at cellular level with a kind of nontoxic preparation.This method and formulation has more physiological, it has prevented acidosic bounce-back, acid gastritis and heart failure, available nutrient when cancerous cell and tumor material have been changed into the healthy cell metabolism, simultaneously treat one or more pernicious and/or non-pernicious (optimum) tumors effectively, as muscular tumor, adenoma, polyp, cyst and general (transitivity) cancer.The water base preparation of processing with electrolysis of cost economy can strengthen the disease resistance in the intracellular environment, the ion of intracellular environment is changed, change the cytochemistry that comprises pHe and pHi, simultaneously the various cancerous cell and the ionic environment that alkalizes be can kill safely, whole body, part and/or cell physiological function changed.The effect of caesium and/or rubidium is included in and has suppressed because the effect of institute's secondary behind a large amount of potassium transmembrane movements that anoxia caused.Such effect comprises that change, the excessive sodium of pH regulation and control gather, reduce the ability of transmembrane potential and minimizing sodium/calcium exchange mechanism.It has low potassium stream at the cell that contains under the oxygen normal condition, even also can be kept under the situation that has caesium or rubidium.In fact, this treatment and preparation have extremely cancer activity (stable, inhibition and regressing tumors) under the situation that all healthy cells are nontoxic in to living things system.Remaining malignant cell can cause the recurrence of disease.The present invention a kind ofly eradicates the useful especially method of remaining tumor cell by obtaining beneficially altering in the cellular environment, and wherein said elimination tumors remaining cell carries out after handling in surgical intervention, chemotherapy and/or radiotherapy or the like.Advantage comprises the speed of effectiveness, and generally in continuous 15 to 30 days, also antagonism usually is the secondary infection that causes cancer patient's main causes of death very effectively, and can improve and the availability of eliminating or significantly reduce bonded hydration of pain and oxygen.

The present invention also to toxicity than having very high effect, thereby make it might on the outpatient basis, implement a part of treatment of cancer, thereby can reduce expenses greatly.

This active component can be formulated into the form that is suitable for carrying out intravenous administration to the cancer patient in latter stage in stupor or late period, or be formulated into the form of injecting the balance solution of effective dose to early stage cancer patient that is applicable to, and can be formulated into outpatient's preparation in some cases as the preparation of oral administration voluntarily, or be formulated into the treatment maintenance dose, giving is had the patient or the high cancer of cancer return danger (heredity, diet, environment or the like) to send out the patient of risk, or is formulated into the maintenance dose that is used for preventing forming the acidosis disease of degeneration.

1. the preparation that is used for ion physiology treatment

The main active component of alkali metal salt is made up of the salt of alkali metal caesium and rubidium.The anionicsite of this salt can be the element or the chemical compound of any nontoxic biological utilisation that does not hinder caesium and rubidium substantially.Can use caesium of the present invention and rubidium compound separately or use to obtain required anticancer and antimetastatic activity and various therapeutical effect with the form of various combinations.

Auxiliary compounds is selected other active component to supply or is strengthened this alkali-metal effect.These other components can also change the ion metabolism of cancerous cell and it can't be survived, and they can reduce the vitality of cancerous cell in incoherent mode, or they can increase the toleration of patient's pair stress relevant with treatment of cancer.As the example of auxiliary compounds, can use angiogenesis inhibitor to come used alkali metal is replenished.When treating or preventing other disease, can select different auxiliary elements.

Water can be handled the availability pure, aseptic and/or that come the enhanced activity composition so that it becomes to used water in the manufacturing preparation of the present invention.Especially, with enough persistent period and voltage water is carried out electrolytic treatments to change its chemistry and physical parameter during fabrication.

Water to electrolytic treatments carries out enough processing so that it has minus oxidation-reduction potential, makes in its energy and the electrophilic toxin, and keeps a kind of redox environment, and active component is available and has very high effectiveness in this environment.Water is preferably handled so that it has 55 to 68 dyne/cm ², most preferably 60 to 68 dyne/cm ²Surface tension and-350 to-560 millivolts ORP, and pH is preferably in 8.5 to 9.7 scope.

2. the application in treatment of cancer

Effect particularly to the observation of ion physiology treatment, comes the effect of preparation and treatment is monitored by the pharmacy observation of standard.Such observation comprises the monitoring to pHe, pHi and whole body pH.

Toxicity depends on the method for transmission, and observation and the treatment relevant with the whole body acidosis with the downright bad speed of tumor cell are used for preventing toxicity.

Physiological stress is used for preventing and treating relevant stress with the observation relevant with the physiological stress of ion physiology treatment and treatment.

3. the application in disease treatment

The observation relevant with effect can be specific to specific disease, but for any known disease, this technical staff who is observed medical domain is known.

4. the application in prophylaxis of cancer

Can come preventative-therapeutic effect is assessed with the statistics character of observation, this be known to those skilled in the art.In theory, the ability that this treatment can be by reducing its environment of cell acidify pre-cancer or carry out the ability of quick copy or the formation that two kinds of abilities that all have prevent cancer with the glycolysis energy metabolism.Can assess each patient's therapeutic efficiency by the reaction that observation is attacked the medicament that can induce the acid generation with one or more at cellular level, for example can induce acid to produce by inducing a kind of of short duration chemical hypoxia.Perhaps, can measure metabolic other artifact of ion at cellular level, as cytoplasmic pH.The toxicity relevant with necrosis is not main cause in prophylactic use or in using.The dosage that is used for preventative purpose is lower, does not therefore have the remarkable stress relevant with ion physiology cancer prevention.

Brief description of drawings

Fig. 1 has described the ORP/pHe (iuntercellular fluid) and the carcinogenic environment of various cancers.

Preferred forms of the present invention

Embodiment preferred provides method and formulation in a kind of body that serious degenerative disease such as cancer is carried out ion treatment, it is used for stablizing effectively tumor, suppresses and regressing tumors, and wherein said tumor non-limiting instance has: pulmonary carcinoma, breast carcinoma, colon cancer, carcinoma of prostate, hepatocarcinoma or the like.

The invention discloses a kind of method and formulation that does not have in the past, it adopts the alkali salt in the electrolyte that meets some electrochemistry and electrophysiology requirement to come suffering from cancer, the human patients or other mammal that have one or more pernicious or non-pernicious (optimum) tumors are treated, and it provides the caesium and/or the rubidium ion for the treatment of effective sufficient dosage for this cell.The present invention also further discloses a kind of being used for and has carried out preventative and keep the method and formulation of administration for various treatment for cancer.

This method and formulation can alkalize extracellular fluid body in the cancerous tumour whole body and partial acidity and change ionic environment in the born of the same parents of this cell.The caesium and the rubidium that are absorbed in the cell tend to be released very slowly.In these ions some are stayed in this cell in the survival period of this cell always, so they have long persistent effect.When the acidity of cancerous tumour was lowered on more normal physiology's level, this patient's metabolic function and immune system (comprising antibody, macrophage or the like) function was incited somebody to action more effective and can be reduced and anticancer duplicating in this tumor mass.This kind reaction is the means of prevent disease recurrence.

Because various secondary infection, the percentage ratio of cancer mortality is very high, and said secondary infection normally has infective microorganism caused by pathogen such as antibacterial with other.If acidosic biological environment bacteria-induction or that tumor produces can suppress patient's immune system, treatment then described here will stimulate this immune system to the level of stable state by recovering pH and pHe, thereby can help the patient to resist various secondary infection and promote this immunologic function reaction.

Manufacture method

Main active the present invention has used salt or its combination of caesium, rubidium in its preparation.Operable cesium salt and rubidium salt is for example cesium chloride and Rubinorm (Ifi). without limitation.The invention discloses method and formulation in a kind of body for the treatment of cancer, it uses by the formed alkaline salt solution of following prescription: MA, wherein MA in aqueous solution basic solution from, thereby formation M+ and A-.M is the alkali metal part, and it can be caesium and/or rubidium.A is an anionicsite, and it can be any compatible nontoxic inorganic matter such as chloride, sulfate, carbonate or phosphate; Or it can be any nontoxic Organic substance such as lactate, citrate or acetate.

If desired the alkali metal part is combined with the anionicsite that is difficult to obtain, then it is easy to realize.For example, alkali-metal hydroxide is combined with required anionic acid form, thereby:

MOH+HA forms MA+H ₂O

Be higher than in the hydrionic situation dissociating, final product can be protonated by part, for example MHCO ₂Or M ₂CO ₂, carbon dioxide bicarbonate or carbonate.Stoichiometry that can be by controlling this reaction or come final product is controlled to obtain required final pH with any known manufacture method.

When needs reduce general acidity, preferred carbonate or can be by metabolic Organic substance.For example, can come the solution of Cesium hydrate. is neutralized,, maybe the Cesium hydrate. of exact magnitude can be mixed with the citric acid of predetermined quantity, with the physiology pH that when dissolving, can obtain to be scheduled to until obtaining nearly neutral pH value with citric acid.If measure to tumor metabolism to monitor by lactate or lactic acid dehydrogenase (LDH), it can preferably avoid using lactate so that background lactate or LDH signal are minimum.For oral formulations and administration, taste will influence one or more anionic selections, and can use one or more flavoring agents.

Control the ratio or the ratio of used caesium and rubidium by the effect considering tumor is stablized, suppressed and disappears and patient's physiological stress.A kind of ionic high dose is being had under the situation of physiological stress, and various being combined in when keeping enough therapeutical effect can be reduced stress.

If necessary, active component can be mixed with the form that can the optimum position in digestive tract discharges feels sick to avoid producing when keeping effectiveness.Perhaps can it be designed to postpone or the form of controllable release with dosage form commonly known in the art and technology.

Second kind of active component selected these active component to replenish with the effect to this active component or strengthened.The example that provides some compositions that potentiation is arranged instructs the doctor as the principle of selecting, but is not NM component will be foreclosed.Can by comprise enhancing by the ion physiology the inductive one-tenth that tends to take place programmed cell death assign to strengthen the effect of caesium/rubidium.Can stimulate cumulative examples for compounds of calcium such as calcium complement agent and magnesium, vitamin D, selenium salt, calcitonin, Calcium ionophore or the like, can obstruct the chemical compound of the elimination of sodium in the cancerous cell such as the inhibitor of monensin or sodium/potassium exchange, can change chemical compound such as nigericin, amiloride and the derivant thereof, 4 of pH regulator characteristic, 4-two isocyanide sulfenyl stilbenes 2,2-disulfonic acid and bafilomycin.Other example is to reduce the chemical compound of tumor cell to the utilization of glucose, as lonidamine, and the activatory chemical compound that can increase programmed cell death independently.The active another kind of composition that can strengthen main active component is that those can stimulate or support immune chemical compound, and especially those are as chemical compounds of the secondary the possibility of result shortage of cancer, as magnesium, zinc, vitamin B2 and B12.But the factor that can replenish the treatment of caesium and/or rubidium is those to work by incoherent method and still can be used to reduce the factor of cancer vigor.These factors comprise not being the various chemotherapy of target with the ion physiology.Because the cancer growth is a kind of balance between propagation of cancer cells and the death, any other factors that reduce breeding or increase cancer cell death all may be useful in the cancer situation of using caesium and/or rubidium to be difficult to treat separately.The application of toxicity chemotherapy is dropped on the minimum degree, and preferably under careful medical supervision, carry out.Another kind of factor of replenishing the therapeutical effect of caesium and/or rubidium is that those can be with the minimized factor of the toxic action of neoplasm necrosis.These factors comprise hydration, other alkalization treatment, the toxic treatment that reduces neoplasm necrosis and nutrition and diet intervention or are suitable for the fill-in of the physiological stress relevant with cancer or neoplasm necrosis.The another kind of composition that replenishes rubidium and/or caesium therapeutical effect is potassium and other inorganic fill-in and diet supplement and antioxidant, is used to compensate potassium and other material damage of causing owing to the slight diuresis of this treatment.Inorganic fill-in comprises trace quantity mineral and ion, also can be used to obtain and keep the pH scope and the cellular metabolism of required body fluid.

If water solidifies crystallization in order to need salt formation as described herein as the purpose of transportation or storage or oral administration, then can come it is prepared with the method for routine, comprise one or more buffered salt.

If preferably, this active component can dissolvedly in advance carry out oral administration, maybe can be made into a kind of solution of absorbing or injecting of being suitable for a kind of carrier liquid of aqueous.For example injection solution should can be it and balance is provided and is that the Chemical composition that injectable is used is prepared with a kind of.Injectable solution generally be included in the isoosmotic aseptic buffer saline of blood in active component.

Used water can be the water with any source of suitable purity.As a kind of non-limiting instance, preferred manufacture method is the water of using by processing as electrolytic processing method, no matter be 100% throughput or independently anode and negative electrode output stream, within a certain period of time (contact) use enough voltage/current or external energy field (electromagnetism, magnetic, radiation, sound wave or the like), the manufacturing that can advantageously provide pharmaceutically useful dosage homogeneity and be suitable for using the various concentrate that are used for various application to carry out through the water of handling like this.The electric flux field can change the electric viscous characteristics of water cluster, thereby this water of reconstruct changes the characteristic of this water.Can set water velocity in the combination of various adjustment such as active component content, the reative cell, hydraulic pressure difference, controlled voltage/current density and time of contact to obtain pharmacy manufacture method accurately.The active component that adds various control concentration dissolves formed suspension, produce a kind of aqueous mixture that is used to carry out electrolytic treatments by adding alkali salt to the aqueous mixture of this reconstruction, it is and belongs to effective quantity or the concentration of treatment for the responsive various cancers of such treatment.

This high density field electrochemically reconstruct this aqueous carrier fluid, make this aqueous carrier fluid activate into less metastable hydrogen bond between the water bunch, said water bunch forms or compiles with around the inorganic ions, thereby its yardstick has been reduced about 50%, or it is processed to obtain less bunch, if necessary, reduce the viscosity and the surface tension of water.As an example, this surface tension is from 73 dyne/cm ²Reduce to 55 and 68 dyne/cm ²Between.Should be noted that reducing surface tension can improve solubilization and ion utilization rate.Reduce the viscosity of water and can improve general hydration or the like.

Syringe via high-speed computer control is introduced in active water soluble salt in the said preparation (caesium and/or rubidium), the injected dose of this syringe control accurate concentration and the water velocity in the preparation, just be expelled in the flow of liquid before one or more these cell reaction chambers, water is by electrochemically in addition reconstruct in said chamber.Be noted that after this electrolytic process injection is included in some inorganic salts and the activator in this concentrate formulation.Select aquatic product parameter so that this product has last acceptable pH of in fact nontoxic physiology and ORP, thereby the optimum utilization and the held water that are provided at active substance in the solution turn usefulness and hindered acid poisoning circulation into.The ORP value of said preparation is-350 values-700 millivolt, is most preferably-350 to-560 millivolts, to avoid healthy cell and to organize contingent infringement.For orally using, this pH can be 8.5 to 9.7, is preferably 8.6 to 9.5.Depend on the characteristic of water and patient's physiological conditions, the availability of generally estimating said preparation and active component is about 2 times or more times of non-brine electrolysis preparation.

Using method

Administering mode can directly be administered into alkaline salt solution of the present invention in the blood flow with any suitable method, wherein said method is as (without limitation) periodically injection, intravenous infusion, rectally, new stomach (neogastric), percutaneous dosing, intraperitoneal administration (peritonial), subcutaneous administration, intramuscular administration, intrathecal drug delivery (intrathecial), sublingual administration or topical, and it can use the miniature osmotic pumps of implantation or other release method or device or the like to carry out administration.Terminology used here " injection " comprises any method that active component or chemical compound can be introduced directly in the blood circulation.Injectable preparation preferably should ooze with blood etc., and should have about pH value of 7.3 to 7.4.Such preparation can be designed to provide delay or sustained release with known preparation and technology in the medical domain.Can be expelled to other position, as be injected directly in the tumor or be expelled near it or the like.

If necessary, basic salt as described herein can also carry out oral administration, perhaps carries out administration with tablet or capsular form, or can be used as the administration of nutrient additive.Oral administration most preferably is to be ingested with suitable carbohydrate.

If necessary, can use other preparation, as comprise the pharmaceutically acceptable compositions of active component, it can be gel, oils, binder/dressing, local lotion, irrigation solution, suppository, coloclysis solution, Sublingual drop or drop dispersant.

Generally speaking, the required prescribed dose of curative effect will depend on following factor, as patient's body weight, age, diet, sex, physical symptom and situation, persistent period and frequency, selected route of administration and various cancer and the progress stage thereof of administration.The deadly limit (caesium) of mice is about 10mEq/ kilogram, but not painful when the 5mEq/ kilogram have some to suppress active.The deadly limit (rubidium) of mice is about 1.93mEq/ kilogram.

Instruct as generality, for treatment for cancer, the dose therapeutically effective of caesium is kilogram every day 0.1 to 5mEq/, and for the prevention of cancer, be per 24 hours 0.005 to 0.1mEq/ kilograms, and, be per 24 hours 0.00001 to 0.01mEq/ kilograms for for the prevention of the degenerative disease of related to cancer.Only in absolute demand, just use the dosage that is higher than the 1mEq/ kilogram, and will carefully monitor symptoms of stress.Teen-age dosage should be lower, according to body weight etc., generally is about 1/2 of adult's dosage.When further not studying, caesium or rubidium should not delivered medicine to anemia of pregnant woman or women breast-feeding their children or baby at present.Directly the dosage that tumor is injected can be quite little, and every day, the per kilogram tumor quality used high dosage to 300mg.Therefore, any stress of required antitumor degree, required alkalization degree and existence has determined to treat the dosage or the quantity of used caesium and/or rubidium.Along with preparation and dosage that the treatment process is renderd a service the best are adjusted.As a kind of non-limiting instance, the patient who suffers from acute acid poison can treat this symptom with rubidium in principle, increases caesium gradually then or any treatment is effectively made up, to obtain to be enough to provide a kind of anti-tumor activity of the neoplasm necrosis that stress not spend.Preferably, selected dosage only provides gentle effectiveness in beginning, suitably increase dosage then to avoid causing shock owing to the excessive release of acid toxin, wherein the excessive release of said acid toxin for example is because the necrosis of massive tumor volume causes.Be noted that then this treatment is the most effective if diet has enough nutrition and can not help acidosic stress.As an example, should be fully edible pH be lower than 2.5 F﹠B, and pH should be lower than beverage and the edible of food that 3 food and low pH can produce mineral acid such as phosphoric acid and minimize or substantially edible this group food.It should be noted that and during treating, encourage edible neutrality or be alkaline food slightly.

Effect is also monitored vital sign such as temperature and blood pressure simultaneously no matter administering mode how, should be monitored patient's tumor.In therapeutic process, should monitor the pH value of patient's saliva, urine and blood, and dosage is carried out suitable adjustment learn the needs of situation to adapt to patient physiological, so that whole body and part tumor locus or whole tumor locus return to 7.3 to 7.4 the appropriate pH e of being about near physiology's level, cause in the cell and the release of the acid that the extracellular is stored.Any possible in, should monitor to fluidic pH within the tumor or from the pH of the effusive blood of tumor with feasible pattern the most accurately.As an example, can or comprise sample of tissue and other suitable scheme of analyzing comes pHe, pHi and other are shown that the level of the index of ion physiologic characters such as tumor sodium, potassium, magnesium and calcium and metabolite such as lactate monitor with nuclear magnetic resonance spectroscopy.Perhaps can carry out the monitoring of Noninvasive to pH with near infrared spectrum.Further the effect indication has the stabilisation of tumor, the labelling that shrinks or disappear and appear at the neoplasm necrosis in blood or other body fluid.With opposite, can reduce pHe and it is dropped to the short-term indication that normal dosage and preparation are the treatment effectiveness at the metabolic carcinous ionic reaction of glycolysis.Be noted that this method and formulation is applicable to the large volume tumor that must be disappeared and be confirmed by lump.PHe and other index underaction are represented a kind of invalid dosage.

The excessive meeting of the dosage of stress rubidium and cesium salt causes physiological stress, and it for example is that caused by slight diuresis or that take place as the result of potassium consumption or is caused by hypopiesia or excessively alkalization of whole body.Need in therapeutic process under the most powerful situation, the upper limit of this dosage is set by the stress symptom.Be noted that this maximal dose must be lower than at the hurtful dosage of this electrolytic equilibrium interference.PH during above-mentioned definite effect is determined as the doctor who practises medicine provides the information that physiological stress is assessed.The pH of blood should not be elevated to the level more than 7.4, and the pH of saliva should only rise to the level more than 7.7 momently, and the pH of urine can not be higher than 7.1 or be lower than 5.0 substantially, perhaps is lower than 4.5 momently.It is numb around myalgia and/or the mouth that pH increases over-drastic another symptom.This shows that needs reduce dosage or stop to treat stress to avoid excess base to poison and brought out momently, and the dosage that increases potassium until this sx to acceptable level.The blood potassium content should not drop to and be lower than tolerable level.Should monitor the creatine level.If should monitor and dehydration takes place it be corrected dehydration.The patient is preferably by abundant aquation before beginning to treat.Should monitor blood pressure.The numb initial stage effect of feeling to show to nervous tissue's ionic condition.Dosage should not surpass those can cause the dosage of very slight feeling of numbness.

Terminology used here " treatment " and " treatment " refer to and can help patient's body antagonism or opposing treatment for cancer, and do not consider whether the ad hoc fashion of effect of basic salt disclosed herein and the cancer of particular patient are disappeared fully.For example, even it can not provide disappearing of life length for specific patient, also be very useful but can prolong survival or help improve treatment of pain.

Come may using of this technology is illustrated with following embodiment.Technical staff in these medical domains will recognize and can to these preparations and method many variations take place with other situation according to each patient's needs.

Embodiment 1

The oral administration dosage of caesium and/or rubidium treatment cancer is in the electrolytic aqueous solution that comprises caesium and/or rubidium salt and other component, the quantity that every vial formulation is 4 ounces is: citric acid caesium and/or citric acid rubidium or its any combination, scope be 500mg/24 hour to 5,000mg/24 hour, preferred 2,500mg/24 hour;

Potassium (being preferably the form of phosphate, gluconate and acetate) 500-2000mg; Calcium 2,500mg; Magnesium citrate 200-1500mg; Sodium chloride; Iodine; Selenium (selenomethionine) 50-200mcg; Vanadium (vanadium oxysulfate) 2-10mg; Zinc gluconate 30-200mg;

Vitamin D 2,000 to 4,000 IU; Vitamin A 2,000 to 5,000 IU; Buffered vitamin C-(L-ascorbic acid) 1,000 to 5,000mg; Malic acid 100-500mg; CO enzyme Q10 ubiquinone 25-50mg; DHEA (dehydroepiandrosterone) 5-50mg; B3 methyl nicotinate 20-30mg; B6 25-100mg; With B12 20-50mg.

Carry out administration with per 24 hours twice forms of 4 ounces.As mentioned above, reply patient's stress and effect are monitored, and treatment is adjusted to obtain to have the tumor regression and the inhibitory reaction of minimum physiological stress.After beginning or favourable reaction period, fail to react and show the component that to consider to use supplemental components or potentiation.

Embodiment 2

Treat by caesium/rubidium that the I.V. administration is carried out

I.V. solution and dosage: the scope of preferred embodiment be generally with the isoosmotic buffer salt of blood in 200mg to 10 grams per liter CsCl, be 1 more electedly, 200mg/ liter, and/or 200mg to 10 grams per liter RbCl.Can also use any combination of CsCl and RbCl under the above-mentioned concentration.

As an example, can carry out successive intravenous drip (2x) by per 24 hours and carry out administration, be generally 250 as required to 1000cc.Be noted that if patient's physiological signs is that death is expected at any moment as the cancer in latter stage of stupor, then need to use higher dosage, for example per 24 hours 1,000cc (2x).Make the patient be under the aquation situation and for it provides the adequate diet that comprises vitamin and mineral fill-in, thereby obtain following oral diet supplement: potassium (being preferably the form of phosphate, gluconate and acetate) 150-1,200mg; Calcium 1,500-2,500mg; Magnesium citrate 200-1500mg; Iodine; Selenium (selenomethionine) 50-200mcg; Vanadium (vanadium oxysulfate) 2-10mg; Zinc gluconate 50-200mg; Vitamin D 2,000 to 4,000 IU; Vitamin A 2,000 to 5,000 IU; Buffered vitamin C-(L-ascorbic acid) 1,000 to 5,000mg; Malic acid 3-5mg; CO enzyme Q 10 ubiquinone 25-50mg; Every day B3 methyl nicotinate 5-20mg; B6 25-100mg; B12 20-50mg.

As mentioned above, the patient is carried out the monitoring of stress and effect, and the positive reaction that has minimum stress to provide is adjusted in treatment.After beginning or favourable reaction period, fail to react and show the component that to consider to use supplemental components or potentiation.

Embodiment 3

Caesium/rubidium disappears and suppresses the dosage and/or the long term maintenance dosage of tumor

With every or capsular quantity is administered once every day or twice, preferably carry out administration with carbohydrate, scope is 250 to 1000 milligrams of every days, is preferably 500mg every day, a kind of non-limiting instance is: citric acid caesium 400mg; Citric acid rubidium 100mg; Potassium (being preferably the form of phosphate, gluconate and acetate) 150-1200mg; Calcium 2,500mg; Magnesium citrate 200-1,500mg; Iodine; Selenium (selenomethionine) 50-200mcg; Vanadium (vanadium oxysulfate) 2-10mg; Zinc gluconate 50-200mg; Vitamin D 2,000 to 4,000 IU; Vitamin A 2,000 to 5,000 IU; Buffered vitamin C-(L-ascorbic acid) 1,000 to 5,000mg; Malic acid 3-5mg; CO enzyme Q10 ubiquinone 25-50mg; Every day B3 methyl nicotinate 5-20mg; B6 25-100mg; B12 20-50mg, DHEA (dehydroepiandrosterone) 5-50mg.

According to per 24 hours dosage or capsule, with the form administration of a slice or a plurality of tablets.Wish to use this preparation and should carry out periodic test with suitable medical approaches to it by the doctor who practises medicine to the very high patient of risk of relapse.The pH of saliva should be preferably 7.37 in 7.2 to 7.4 scope, so that the integrity of gene and reparation maximization.If have some can by medical symptom that this treatment worsened as with harm or unusual mineral absorption or the relevant symptom of hypotension, then point out stress is monitored.

The character that application had of new method of the present invention and preparation is appreciated that the application comprises scope and the others wideer than these cited embodiment.

The fact of being discussed in disclosure thing and theory are to be used for instructing the reader how to use the present invention.But theory of the present invention is not the part of claim, and the inventor does not wish to limit the invention with any explanation particular theory of the present invention.In fact, can reckon with that fully supporting theory of the present invention will constantly develop with maturation along with oncology's the reach of science.

Though invention has been described in conjunction with embodiment preferred, those skilled in the art obviously can also carry out various modifications, variation or replacement to it, and these modifications, variation or replacement can not break away from the spirit and scope of the invention.Therefore, scope of the present invention only is subjected to the restriction of claims and legal equivalents thereof.

Claims

1. composition of matter, it comprises the aqueous base metal salt solution with following general formula that is used for the treatment of mammalian cancer:

MA (aq), wherein

MA dissociates in water and forms M+ and A-;

M a kind ofly is selected from the alkali metal of caesium and rubidium and comprises caesium separately and rubidium or comprise caesium and rubidium with its any combining form; With

A is a kind of anion that is selected from chloride, sulfate, carbonate, phosphate, lactate, citrate and acetate.

2. composition of matter as claimed in claim 1, it further comprises:

The material that at least a stimulation calcium gathers, said material is selected from vitamin D, selenium salt, calcitonin and Calcium ionophore;

The material that sodium is eliminated at least a minimizing cancerous cell, said material is selected from monensin and sodium/potassium exchanger inhibitor;

The material of at least a change pH, said material is selected from nigericin, amiloride, 4,4 '-two isocyanide sulfenyl stilbenes 2,2-disulfonic acid and bifilomycin, the quantity of this material is enough to reduce the acidity at patient tumors position and the acidity of whole body; With

At least a reduction tumor cell is to the material of the utilization of glucose.

3. composition of matter as claimed in claim 1, it further comprises:

At least a quantity is enough to increase the material of the activation of programmed cell death in patient's body;

At least a quantity is enough to the material of stimulating immune system, and said material is selected from magnesium, zinc, vitamin B2 and vitamin B12;

At least a by incoherent mode but the mode of survival that can be used for reducing cancer to the material that the treatment of caesium and/or rubidium replenishes, comprise in the prior art well-known and in chemotherapy the physiological chemical compound of not targeting ion commonly used; With

At least a quantity is enough to compensate the material of the potassium loss that any diuresis owing to this treatment causes, the mineral fill-in that it is selected from potassium, antioxidant and comprises trace quantity mineral.

4. the dosage that composition of matter as claimed in claim 1, wherein said solution are suitable for four ounces carries out oral administration one day twice, and said alkali metal salt is that quantity is 250mg to 2, the citric acid caesium of 500mg and/or citric acid rubidium or its any combination; And wherein said solution further comprises 125 to 1000mg the potassium salt that is selected from potassium phosphate, potassium gluconate and potassium acetate; 1,250mg calcium; 100 to 1, the 250mg magnesium citrate; Iodine; 50mcg to 150mcg selenomethionine; 1 to 5mcg vanadium oxysulfate; 25 to 100mg zinc gluconate; 1,000 to 2,000 IU vitamin D; 1,000 to 2,500 IU vitamin A; 500 to 2, the buffered vitamin C of 500mg (L-ascorbic acid); 50 to 250mg malic acids; 12.5 to 25mg COq; 2.5 to 25mg DHEA (dehydroepiandrosterone); 10 to 15mg B3 methyl nicotinates; 12.5 to 50mg B6; With 10 to 25mcg B12.

5. composition of matter as claimed in claim 1, wherein said alkali metal salt is cesium chloride and/or Rubinorm (Ifi)., it can use separately or with its any being used in combination, its quantity is 200mg to 10 gram basic salt in every premium on currency, and said solution is buffer and oozes with blood etc. that said solution is suitable for carrying out administration by intravenous drip, carried out twice instillation in per 24 hours, according to patient's needs, quantitative range is 250 to 2,000cc.

6. composition of matter as claimed in claim 5, it further comprises 125 to the 1000mg potassium salt that are selected from potassium phosphate, potassium gluconate and potassium acetate; 1,250mg calcium; 100 to 1, the 250mg magnesium citrate; Iodine; 50mcg to 150mcg selenomethionine; 1 to 5mcg vanadium oxysulfate; 25 to 100mg zinc gluconate; 1,000 to 2,000 IU vitamin D; 1,000 to 2,500 IU vitamin A; 500 to 2, the buffered vitamin C of 500mg (L ascorbic acid); 50 to 250mg malic acids; 12.5 to 25mg COq; 2.5 to 25mg DHEA (dehydroepiandrosterone); 10 to 15mg B3 methyl nicotinates; 12.5 to 50mg B6; With 10 to 25mcgB12.

7. composition of matter as claimed in claim 1, wherein said alkali metal salt comprise 400mg citric acid caesium and 100mg citric acid rubidium; And further comprise 125 to the 1000mg potassium salt that are selected from potassium phosphate, potassium gluconate and potassium acetate; 1,250mg calcium; 100 to 1, the 250mg magnesium citrate; Iodine; 50mcg to 150mcg selenomethionine; 1 to 5mcg vanadium oxysulfate; 25 to 100mg zinc gluconate; 1,000 to 2,000 IU vitamin D; 1,000 to 2,500 IU vitamin A; 500 to 2, the buffered vitamin C of 500mg (L ascorbic acid); 50 to 250mg malic acids; 12.5 to 25mg COq; 2.5 to 25mg DHEA (dehydroepiandrosterone); 10 to 15mg B3 methyl nicotinates; 12.5 to 50mg B6; 10 to 25mcgB12, and wherein aqueous solution is handled tablet or the powder type done that is used for being administered orally in for twice every day the cancer patient to be mixed with.

8. method for the treatment of mammalian cancer, the step that this method comprises has:

Give a kind of aqueous base metal salt solution of patient's administering therapeutic effective dose, wherein said basic salt has following general formula:

MA (aq) and MA dissociate in water and form M+ and A-;

M a kind ofly is selected from the alkali metal of caesium and rubidium and comprises caesium separately and rubidium or comprise caesium and rubidium with its combination; With

9. method as claimed in claim 8, wherein said aqueous base metal salt solution further comprises the material that at least a stimulation calcium gathers, said material is selected from vitamin D, selenium salt, calcitonin and Calcium ionophore, with the material that the sodium at least a reduction cancerous cell is eliminated, said material is selected from monensin and sodium/potassium exchanger inhibitor.

10. method as claimed in claim 8, wherein said aqueous base metal salt solution further comprises:

The material of at least a change pH, said material is selected from nigericin, amiloride, 4,4 '-two isocyanide sulfenyl stilbenes 2,2-disulfonic acid and bifilomycin, the quantity of this material is enough to reduce the acidity at patient tumors position and the acidity of whole body;

At least a reduction tumor cell is to the material of the utilization of glucose, and comprises that further at least a quantity is enough to increase the material of the activation of programmed cell death in patient's body;

At least a quantity is enough to the material of stimulating immune system, and said material is selected from magnesium, zinc, vitamin B2 and vitamin B12; With the material that at least a quantity is enough to compensate the potassium loss that any diuresis owing to this treatment causes, the mineral fill-in that it is selected from potassium, antioxidant and comprises trace quantity mineral.

11. method as claimed in claim 8, wherein said alkali metal salt soln is by oral administration.

12. method as claimed in claim 8, wherein said alkali metal salt soln carries out administration by injection.

13. method as claimed in claim 8, wherein said alkali metal salt soln carries out administration by said material is incorporated in the body cavity.

14. method as claimed in claim 8, wherein said alkaline salt solution is applied directly on the carcinous tumor.

15. method for the treatment of mammalian cancer, this method comprises the step of a kind of aqueous base metal salt solution of drug treatment effective dose, wherein said alkali metal salt is selected from citric acid caesium, cesium chloride, citric acid rubidium and Rubinorm (Ifi)., this salt can be used separately or with its any applied in any combination, quantitative range is 250mg to 2,500mg; And wherein said solution comprises that further 125 to 1000mg are selected from the potassium salt of potassium phosphate, potassium gluconate and potassium acetate; 1,250mg calcium; 100 to 1, the 250mg magnesium citrate; Iodine; 50mcg to 150mcg selenomethionine; 1 to 5mcg vanadium oxysulfate; 25 to 100mg zinc gluconate; 1,000 to 2,000 IU vitamin D; 1,000 to 2,500 IU vitamin A; 500 to 2, the buffered vitamin C of 500mg (L-ascorbic acid); 50 to 250mg malic acids; 12.5 to 25mg COq; 2.5 to 25mg DHEA (dehydroepiandrosterone); 10 to 15mg B3 methyl nicotinates; 12.5 to 50mg B6; With 10 to 25mcg B12.

16. method as claimed in claim 15, wherein said alkali metal salt are citric acid caesium and citric acid rubidium, it can be used separately or with its any applied in any combination, quantitative range is per four ounces of solution 250 to 2, and 500mg carries out oral administration twice every day.

17. method as claimed in claim 15, wherein said alkali metal salt soln comprises watersoluble chlorinated caesium and/or Rubinorm (Ifi)., it can use separately or can be employed with the form of its any combination, quantitative range is every premium on currency 200mg to 10 gram basic salt, said solution is buffer and oozes with blood etc. that according to patient's needs, said solution carries out administration by intravenous drip, every day 250 to 2,000cc.

18. method as claimed in claim 17, wherein said aqueous base metallic solution comprise that further 125 to 1000mg are selected from the potassium salt of potassium phosphate, potassium gluconate and potassium acetate; 1,250mg calcium; 100 to 1, the 250mg magnesium citrate; Iodine; 50mcg to 150mcg selenomethionine; 1 to 5mcg vanadium oxysulfate; 25 to 100mg zinc gluconate; 1,000 to 2,000 IU vitamin D; 1,000 to 2,500 IU vitamin A; 500 to 2, the buffered vitamin C of 500mg (L-ascorbic acid); 50 to 250mg malic acids; 12.5 to 25mg COq; 2.5 to 25mg DHEA (dehydroepiandrosterone); 10 to 15mg B3 methyl nicotinates; 12.5 to 50mg B6; With 10 to 25mcg B12.

19. method as claimed in claim 15, wherein said alkali metal salt comprise 400mg citric acid caesium and 100mg citric acid rubidium; Wherein said solution further comprises 125 to the 1000mg potassium salt that are selected from potassium phosphate, potassium gluconate and potassium acetate; 1,250mg calcium; 100 to 1, the 250mg magnesium citrate; Iodine; 50mcg to 150mcg selenomethionine; 1 to 5mcg vanadium oxysulfate; 25 to 100mg zinc gluconate; 1,000 to 2,000 IU vitamin D; 1,000 to 2,500 IU vitamin A; 500 to 2, the buffered vitamin C of 500mg (L ascorbic acid); 50 to 250mg malic acids; 12.5 to 25mg COq; 2.5 to 25mg DHEA (dehydroepiandrosterone); 10 to 15mg B3 methyl nicotinates; 12.5 to 50mg B6; 10 to 25mcgB12; Said method comprises further said solution is mixed with and is used for the tablet of doing of oral administration and the step of Sprinkle Caps that said tablet or capsule are suitable for mammalian cancer is carried out long-term treatment.

20. the method for treatment mammalian cancer as claimed in claim 8, this method further comprise the step that pH is monitored and treatment is adjusted, so that whole body pH, tumor pHe and tumor pHi fall in the predetermined scope.