CN1488943A - Method for preparing diaghostic reagent of human immunodeficiency virus antibody - Google Patents
Method for preparing diaghostic reagent of human immunodeficiency virus antibody Download PDFInfo
- Publication number
- CN1488943A CN1488943A CNA021373574A CN02137357A CN1488943A CN 1488943 A CN1488943 A CN 1488943A CN A021373574 A CNA021373574 A CN A021373574A CN 02137357 A CN02137357 A CN 02137357A CN 1488943 A CN1488943 A CN 1488943A
- Authority
- CN
- China
- Prior art keywords
- human immunodeficiency
- immunodeficiency virus
- carrier
- reagent
- bag
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Coating fluid is prepared on a carrier by using pH=9.5 carbonate buffer solution to dilute 1/2 antigen of human immunol-deficiency virus adopted in prior art properly. The said carriers may be polystyrene, polyethylene, cellulose, cellulose nitrate and acetyl cellulose. Coated carriers are placed for 10-18 hrs. at temperature 2-8 deg.C, or for 1-3 hrs. at temperature 37 deg.C. Next, the carriers are cleaned up in cleaning solution fully. Finally, the carriers are dried for 4 hrs. at temperature 37 deg.C.. The precoated carriers together with additive dryer in sack are sealed by plastic bag and deposited at temperature 2-8 deg.C. Comparing with prior art, the invention possesses advantages of easy, quick, sensitivity, reliable operation and economic.
Description
Technical field
The present invention relates to a kind of immune biochemical field, particularly a kind of human immunodeficiency virus is a kind of preparation method of diaghostic reagent of human immunodeficiency virus antibody specifically.
Background technology
Overflow of drugs has become one of the most serious public hazards in the world today, because the patient of druggy infection human immunodeficiency virus is more and more, whether therefore when blood transfusion, carrying out to the blood donor, the detection of aids infection virus is vital.
Summary of the invention
Technical matters to be solved by this invention is: because in the prior art, whether detect costing an arm and a leg of aids infection virus, and the time is long, the present invention provides a kind of preparation method of diaghostic reagent of human immunodeficiency virus antibody for the technical scheme that above-mentioned technical matters adopted in the solution prior art, described method is human immunodeficiency virus-1/2 antigen that adopts in the prior art, make coating buffer with the suitable dilution of the carbonate buffer solution of PH=9.5, the coating buffer bag is made on carrier thus, wherein, described carrier can be a polystyrene, tygon, cellulose, cellulose nitrate, cellulose acetate, place temperature under 2-8 ℃ environment, to deposit 10-18 hour in the carrier behind the bag quilt, perhaps place temperature under 37 ℃ environment, to deposit 1-3 hour, fully wash its carrier with cleansing solution then, be placed on temperature again under 37 ℃ environment dry 4 hours, promptly become pre-bag suppressed by vector, should the additional sack drying agent of pre-bag suppressed by vector with plastic bag sealing after, deposit under 2-8 ℃ environment in temperature and to get final product.During use, above-mentioned pre-bag suppressed by vector is equipped with corresponding reagent such as enzyme mark bond, HIV-1/2 positive serum, HIV-1/2 negative serum, sample diluting liquid, cleansing solution, substrate solution and stop buffer, can carries out the detection of human immune defect virus antibody to blood donor or the person of having a medical check-up, suspicious crowd simultaneously by primary sample.The present invention and prior art contrast, have method of operating easy, quick, sensitive, reliably reach the significant advantage of economic dispatch.
Embodiment:
Embodiment 1
With the polystyrene micropore reaction plate is carrier, wraps quilt in advance: get HIV-1/2 antigen, use PH=9.5, carbonate buffer solution suitably dilution is made coating buffer, and on micro reaction plate, every hole adds coating buffer 100ul, put 4 ℃ 10-18 hour, or 37 ℃ after 3 hours, discard coating buffer, wash 3 times with cleansing solution, put 37 ℃ after 4 hours, add two drying prescription of being responsible for a task until it is completed, be loaded in the polybag and seal, put 2-8 ℃ of storage.Bag is equipped with reagent corresponding again by good micro reaction plate: enzyme mark bond, positive control, negative control thing, sample diluting liquid, cleansing solution, substrate solution (A, B), stop buffer promptly can be used for treating the detection of test sample product.
Embodiment 2
With the nitrocellulose membrane is that carrier wraps quilt in advance: get HIV-1/2 antigen, suitably dilute with the PH-9.5 carbonate buffer solution and make coating buffer.Get nitrocellulose membrane and be cut into suitable size, be divided into required little lattice, draw coating buffer, add in right amount in the middle of every lattice, after waiting to do, add drying agent 1 bag and be sealed in the polybag 2-8 ℃ of storage together with micro sample adding appliance.Above-mentioned bag is equipped with reagent corresponding by good nitrocellulose membrane: enzyme mark bond, positive control, negative control, sample diluting liquid, cleansing solution, substrate solution (A, B), stop buffer promptly can be used for treating the detection of test sample product.
Embodiment 3
During detection,, be used with corresponding reagent such as enzyme mark bond HIV-1/2 positive serum, HIV-1/2 negative serum, sample diluting liquid, cleansing solution, substrate solution (A, B) and stop buffers with the pre-bag suppressed by vector for preparing.During concrete operations, get a pre-bag suppressed by vector such as pre-bag by micro reaction plate, after 21 times of dilutions of testing sample usefulness sample diluting liquid, inject in the hole on the pre-bag suppressed by vector, its every hole is annotated and is added 100ul, add blank 1 hole, each 2 hole of 2 kinds of positive controls, each 100ul of negative control 3 holes simultaneously, put 37 ℃ and discard above-mentioned reactant after 1 hour, with cleansing solution flushing 4 times, the every hole 100ul of enzyme-added again mark bond puts 37 ℃ of washings after 1 hour 4 times, add substrate solution 100ul put 37 ℃ 30 minutes, add stop buffer 50ul.Survey every hole OD value with microplate reader in 450um.Positive with the average OD value of sample well OD value＞2.1 * negative control for this test, illustrate that promptly these test sample product infected HIV-1/2.
Claims (3)
1, a kind of preparation method of diaghostic reagent of human immunodeficiency virus antibody, it is characterized in that: described method is human immunodeficiency virus-1/2 antigen that adopts in the prior art, make coating buffer with the suitable dilution of the carbonate buffer solution of PH=9.5, the coating buffer bag is made on carrier thus, place temperature under 2-8 ℃ environment, to deposit 10-18 hour in the carrier behind the bag quilt, fully wash its carrier with cleansing solution then, be placed on temperature again under 37 ℃ environment dry 4 hours, promptly become pre-bag suppressed by vector, should the additional sack drying agent of pre-bag suppressed by vector with plastic bag sealing after, deposit under 2-8 ℃ environment in temperature and to get final product.
2, the preparation method of a kind of diaghostic reagent of human immunodeficiency virus antibody according to claim 1 is characterized in that: will wrap by after carrier place or place temperature under 37 ℃ environment, to deposit 1-3 hour.
3, the preparation method of a kind of diaghostic reagent of human immunodeficiency virus antibody according to claim 1 is characterized in that: said carrier is polystyrene, tygon, cellulose, cellulose nitrate or cellulose acetate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA021373574A CN1488943A (en) | 2002-10-09 | 2002-10-09 | Method for preparing diaghostic reagent of human immunodeficiency virus antibody |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA021373574A CN1488943A (en) | 2002-10-09 | 2002-10-09 | Method for preparing diaghostic reagent of human immunodeficiency virus antibody |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1488943A true CN1488943A (en) | 2004-04-14 |
Family
ID=34146984
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA021373574A Pending CN1488943A (en) | 2002-10-09 | 2002-10-09 | Method for preparing diaghostic reagent of human immunodeficiency virus antibody |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1488943A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106018805A (en) * | 2016-07-15 | 2016-10-12 | 中南大学湘雅三医院 | Non-diagnosis-targeted HIV antibody immunity detecting method and kit |
-
2002
- 2002-10-09 CN CNA021373574A patent/CN1488943A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106018805A (en) * | 2016-07-15 | 2016-10-12 | 中南大学湘雅三医院 | Non-diagnosis-targeted HIV antibody immunity detecting method and kit |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101377490B (en) | Magnetic microparticle separating chemiluminescence immune analysis determination reagent kit for detecting related sign object and preparing method thereof | |
| CN102614948B (en) | Micro-fluidic chip and manufacturing method thereof | |
| EP0084531B1 (en) | Assay for viruses | |
| Widell et al. | Detection of hepatitis C core antigen in serum or plasma as a marker of hepatitis C viraemia in the serological window‐phase | |
| CN1030300A (en) | Utilize the enzyme-labeled immunity detection method to measure antigen and/or antibody in the human body simultaneously | |
| CN101551397A (en) | Reagent box for detecting nature of third type hepatitis virus antibody by using chemiluminescence method | |
| CN101178404B (en) | Human immunodeficiency virus antibody chemiluminescence immune analyzing diagnose reagent box and method of producing the same | |
| CN101551394A (en) | Method for detecting third type hepatitis virus antibody by using magnetic micro-particle as transporting species | |
| CN108254554A (en) | Influenza A virus IgA antibody Immunofluorescence test test strips and preparation method thereof, detection method and application | |
| CN1254685C (en) | Test paper tape for detecting blood HIV 1/2 antibody by colloidal gold chromatographic analysis and its preparing method | |
| Poltavchenko et al. | Multiplex method for initial complex testing of antibodies to blood transmitted diseases agents | |
| CN101398430A (en) | Tumor-associated antigen 153 magnetic microparticle chemiluminescence immune assay determination kit and method for making same | |
| CN1488943A (en) | Method for preparing diaghostic reagent of human immunodeficiency virus antibody | |
| CN202049160U (en) | COxB (Coxsackievirus B) IgG/IgM (immunoglobulin G/immunoglobulin M) detection reagent kit by enzyme-linked immunosorbent assay method | |
| CN102590520A (en) | EDDP (ethylenediamine-dimethylphosphinic acid) colloidal gold detection kit and preparation method thereof | |
| CN1570638A (en) | SARS virus antibody detecting method, rapid diagnosis kit and preparation method | |
| CN102818892B (en) | Detection kit for prostate specific antigen and preparation method thereof | |
| CN109884294B (en) | Preparation method of high-precision fluorescent immune test strip | |
| CN101533025A (en) | Chemoluminescent immunoassay kit of hepatitis A virus IgM antibody and preparation method thereof | |
| CN106771218A (en) | A kind of kit of p24 antigens of detection HIV 1 and its application | |
| CN1062662C (en) | Process for preparing human immunodeficiency virus and hepatitis C virus antibody united diagnostic reagent | |
| CN102707073A (en) | Enzyme linked immunosorbent assay kit for detecting metandienone and using method of enzyme linked immunosorbent assay kit | |
| CN101082625B (en) | Method for measuring specificity HIV antibody generated by human immunodeficiency virus (1+2 type) different antigen epitope | |
| CN1122845C (en) | Test strip for detecting more antigens of typhoid bacillus | |
| CN102445540A (en) | Kit for quantitatively detecting HCV-aAg concentration in human serum by virtue of polystyrene microspheres |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |