CN1488637A - Method for preparing total aglycone extract of radix picrorrhizae - Google Patents
Method for preparing total aglycone extract of radix picrorrhizae Download PDFInfo
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- CN1488637A CN1488637A CNA031563732A CN03156373A CN1488637A CN 1488637 A CN1488637 A CN 1488637A CN A031563732 A CNA031563732 A CN A031563732A CN 03156373 A CN03156373 A CN 03156373A CN 1488637 A CN1488637 A CN 1488637A
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- rhizoma picrorhizae
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- organic solvent
- anhydrous organic
- picrorhiza rhizome
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Abstract
The invention refers to a manufacturing method of medical Radix picrorrhizae total glucoside extracted substances. The method uses water less organic solvent to extract the dry and coarse extracted substances. The waterless organic extracted liquid is condensed to be dry, and gets the product.
Description
Technical field
The present invention relates to a kind of preparation method of medicinal raw material picrorhiza rhizome total glycoside extract.
Background technology
Picrorhiza rhizome total glycoside extract is the effective ingredient in Chinese extract of extraction separation from Chinese medicine Rhizoma Picrorhizae (Picrorrhiza Scrophulariflora Pennell).Wherein main effective constituent is iridoid such as Rhizoma Picrorhizae glucoside II, kutkin I and other micro-iridoid, contains a small amount of cucurbitacin glycoside composition in addition.Picrorhiza rhizome total glycoside extract has pharmacologically actives such as resistance of hepatitis B, anti-liver injury, can be used for treating hepatitis and other liver injury.The existing method that prepare picrorhiza rhizome total glycoside extract has two kinds, and a kind of is that the Rhizoma Picrorhizae crude extract is crossed polyamide column, and collection contains the Rhizoma Picrorhizae total glucosides part, is concentrated into dried picrorhiza rhizome total glycoside extract crude product.Total glycosides crude product extract concentrated solution can contain Rhizoma Picrorhizae glucoside II, the higher picrorhiza rhizome total glycoside extract of kutkin I through alumina treatment.Polymeric amide is used to prepare Chinese medical extract and does not pass through safety evaluation, and technology is also more loaded down with trivial details.Another kind is that the Rhizoma Picrorhizae crude extract is crossed macroporous resin treatment, obtains containing the picrorhiza rhizome total glycoside extract of Rhizoma Picrorhizae glucoside II more than 40%.Total glycosides content of the picrorhiza rhizome total glycoside extract that this method is prepared can not reach more than 50%.
Summary of the invention
Purpose of the present invention is exactly a kind of new operational path that provides for the defective that overcomes prior art.This operational path is that to have technology simple, and cost is low, and what the total glycosides content of extract was high prepares the method for picrorhiza rhizome total glycoside extract from Rhizoma Picrorhizae.
Purpose of the present invention can be achieved through the following technical solutions: a kind ofly prepare the method for Rhizoma Picrorhizae total glucosides effective part extract from Rhizoma Picrorhizae, it is characterized in that the dried crude extract with Rhizoma Picrorhizae or Rhizoma Picrorhizae extracts with anhydrous organic solvent.The anhydrous organic solvent extracting solution is concentrated into dried, promptly gets picrorhiza rhizome total glycoside extract, and the content of its Rhizoma Picrorhizae glucoside II is greater than 50%.Anhydrous organic solvent can be methyl alcohol, ethanol, propyl alcohol, Virahol, acetone, ethyl acetate, butylacetate, tetrahydrofuran (THF), perhaps the mixed solvent of being made up of them.
Embodiment
[embodiment one] gets Rhizoma Picrorhizae 1kg, water 8000ml decocted 0.5 hour, the dregs of a decoction add water 6000ml and decocted 1 hour, merge decoction liquor, decoction liquor is crossed D101 type macroporous resin column (the D101 type macroporous resin column 2kg that has handled well, column diameter 10cm), be washed to colourless after, the ethanol elution with 70% is to colourless, 70% ethanol eluate vacuum concentration is to doing, residue is used dehydrated alcohol 2000ml refluxing extraction 1 hour, and leaching extracting solution, residue are used dehydrated alcohol 1000ml refluxing extraction 1 hour again, leaching extracting solution and united extraction liquid, vacuum concentration gets picrorhiza rhizome total glycoside extract 85.4 grams, the content 81.3% of Rhizoma Picrorhizae glucoside II to doing.
[embodiment two] get Rhizoma Picrorhizae 1kg, with 70% ethanol 5000ml refluxing extraction 2 hours, the dregs of a decoction were with 70% ethanol 3000ml refluxing extraction 1 hour, united extraction liquid, vacuum concentration is to 1000ml, add alcohol 3000ml, left standstill 12 hours, the elimination precipitation, the supernatant liquor vacuum concentration is to doing, residue is with the mixed solution 3000ml refluxing extraction 1.5 hours of dehydrated alcohol-ethyl acetate (3: 7), and leaching extracting solution, residue are used the mixed solution 2000ml refluxing extraction 1 hour of dehydrated alcohol-ethyl acetate (3: 7) again, leaching extracting solution and united extraction liquid, vacuum concentration gets picrorhiza rhizome total glycoside extract 89.5 grams, the content 73.2% of Rhizoma Picrorhizae glucoside II to doing.
[embodiment three] get Rhizoma Picrorhizae 1kg, with 50% ethanol 6000ml refluxing extraction 2 hours, the dregs of a decoction were with 50% ethanol 3000ml refluxing extraction 1 hour, united extraction liquid, vacuum concentration is to 1000ml, and do not contain ethanol, with water saturated propyl carbinol 1500ml, 1000ml extraction 2 times, the butanol extraction liquid vacuum concentration is to doing, the mixed solution 2000ml refluxing extraction of residue usefulness dehydrated alcohol-ethyl acetate (1: 1) 1.5 hours, the leaching extracting solution, residue is used the mixed solution 1000ml refluxing extraction 1 hour of dehydrated alcohol-ethyl acetate (3: 7) again, leaching extracting solution and united extraction liquid, and vacuum concentration is to doing, get picrorhiza rhizome total glycoside extract 82.3 grams, the content 78.4% of Rhizoma Picrorhizae glucoside II.
[embodiment four] get Rhizoma Picrorhizae 1kg, and with 85% ethanol 4000ml refluxing extraction 3 hours, the dregs of a decoction were with 85% ethanol 3000ml refluxing extraction 2 hours, united extraction liquid, vacuum concentration is to doing, and residue extracts 4 times with ethyl acetate backflow, uses ethyl acetate 2000ml at every turn, each 1.5 hours, incline while hot and get extracting solution, united extraction liquid, vacuum concentration is to doing, get picrorhiza rhizome total glycoside extract 65.7 grams, the content 60.4% of Rhizoma Picrorhizae glucoside II.
[embodiment five] get Rhizoma Picrorhizae 1kg, with 80% methanol aqueous solution 4000ml refluxing extraction 3 hours, the dregs of a decoction were with 80% methanol aqueous solution 3000ml refluxing extraction 2 hours, united extraction liquid, vacuum concentration is to doing, the mixed solution 2000ml refluxing extraction of residue usefulness dehydrated alcohol-ethyl acetate (7: 3) 1.5 hours, the leaching extracting solution, residue is used the mixed solution 1000ml refluxing extraction 1 hour of dehydrated alcohol-ethyl acetate (7: 3) again, leaching extracting solution and united extraction liquid, vacuum concentration gets picrorhiza rhizome total glycoside extract 92.5 grams, the content 70.5% of Rhizoma Picrorhizae glucoside II to doing.
[embodiment six] get Rhizoma Picrorhizae 1kg, use dehydrated alcohol 4000ml refluxing extraction 3 hours, and the dregs of a decoction are used dehydrated alcohol 3000ml refluxing extraction 2 hours, united extraction liquid, vacuum concentration gets picrorhiza rhizome total glycoside extract 101.5 grams, the content 65.5% of Rhizoma Picrorhizae glucoside II to doing.
[embodiment seven] get Rhizoma Picrorhizae 1kg, use methyl alcohol 3000ml refluxing extraction 3 hours, and the dregs of a decoction are used methyl alcohol 2000ml refluxing extraction 2 hours, and united extraction liquid, vacuum concentration get picrorhiza rhizome total glycoside extract 122.5 and restrain the content 53.4% of Rhizoma Picrorhizae glucoside II to doing.
[embodiment eight] get Rhizoma Picrorhizae 1kg, and with acetone 4000ml refluxing extraction 3 hours, the dregs of a decoction were with acetone 3000ml refluxing extraction 2 hours, united extraction liquid, vacuum concentration as for, picrorhiza rhizome total glycoside extract 98.7 grams, the content 63.4% of Rhizoma Picrorhizae glucoside II.
Claims (5)
1, a kind ofly prepares the method for picrorhiza rhizome total glycoside extract, it is characterized in that the dried crude extract of Rhizoma Picrorhizae or Rhizoma Picrorhizae is extracted with anhydrous organic solvent from Rhizoma Picrorhizae.The anhydrous organic solvent extracting solution is concentrated into dried, promptly gets picrorhiza rhizome total glycoside extract.
2, be acetone according to the described anhydrous organic solvent of claim 1.
3, be dehydrated alcohol according to the described anhydrous organic solvent of claim 1.
4, be ethyl acetate according to the described anhydrous organic solvent of claim 1.
5, be the mixed solvent that ethyl acetate and dehydrated alcohol are formed according to the described anhydrous organic solvent of claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031563732A CN1488637A (en) | 2003-09-05 | 2003-09-05 | Method for preparing total aglycone extract of radix picrorrhizae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CNA031563732A CN1488637A (en) | 2003-09-05 | 2003-09-05 | Method for preparing total aglycone extract of radix picrorrhizae |
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| CN1488637A true CN1488637A (en) | 2004-04-14 |
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| CNA031563732A Pending CN1488637A (en) | 2003-09-05 | 2003-09-05 | Method for preparing total aglycone extract of radix picrorrhizae |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1325079C (en) * | 2005-03-28 | 2007-07-11 | 拓普中兴(北京)医药科技发展有限公司 | Chinese medicine picrorhiza extract possessing anti hepatitis B virus activity and its preparation method |
| CN102462772A (en) * | 2010-11-15 | 2012-05-23 | 天津药物研究院 | Application of extract of total glucosides of picrorhiza in preparing medicines for preventing and treating fatty liver |
| CN102481328A (en) * | 2009-08-12 | 2012-05-30 | M·么达萨尼 | Extract Of Picrorhiza Kurroa Plants And A Process For Making The Same |
| CN103792307A (en) * | 2012-10-26 | 2014-05-14 | 中国石油化工股份有限公司 | Extraction liquid for testing of phthalic acid ester content in polyvinyl chloride (PVC) product and its applications |
| CN104693253A (en) * | 2015-03-30 | 2015-06-10 | 济南东源生物医药技术有限公司 | Industrial preparation method for high-purity 6'-O-trans-cinnamyl catalpol |
-
2003
- 2003-09-05 CN CNA031563732A patent/CN1488637A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1325079C (en) * | 2005-03-28 | 2007-07-11 | 拓普中兴(北京)医药科技发展有限公司 | Chinese medicine picrorhiza extract possessing anti hepatitis B virus activity and its preparation method |
| CN102481328A (en) * | 2009-08-12 | 2012-05-30 | M·么达萨尼 | Extract Of Picrorhiza Kurroa Plants And A Process For Making The Same |
| CN102462772A (en) * | 2010-11-15 | 2012-05-23 | 天津药物研究院 | Application of extract of total glucosides of picrorhiza in preparing medicines for preventing and treating fatty liver |
| CN102462772B (en) * | 2010-11-15 | 2014-03-12 | 天津药物研究院 | Application of extract of total glucosides of picrorhiza in preparing medicines for preventing and treating fatty liver |
| CN103792307A (en) * | 2012-10-26 | 2014-05-14 | 中国石油化工股份有限公司 | Extraction liquid for testing of phthalic acid ester content in polyvinyl chloride (PVC) product and its applications |
| CN104693253A (en) * | 2015-03-30 | 2015-06-10 | 济南东源生物医药技术有限公司 | Industrial preparation method for high-purity 6'-O-trans-cinnamyl catalpol |
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