CN1484959A - Sterilization formulation and wet tissue, preparation method and use - Google Patents
Sterilization formulation and wet tissue, preparation method and use Download PDFInfo
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- CN1484959A CN1484959A CNA021372438A CN02137243A CN1484959A CN 1484959 A CN1484959 A CN 1484959A CN A021372438 A CNA021372438 A CN A021372438A CN 02137243 A CN02137243 A CN 02137243A CN 1484959 A CN1484959 A CN 1484959A
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- wet tissue
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Abstract
It relates to a bactericide, comprising lysostaphin 0.0001-0.1wt%, lysozyme 10-50wt%, stabilizer 1-15wt%, surfactant 0.1-0.5wt%, water. It also relates to an antiseptic wet towel, its preparation method and applications in treatment and prevention of mammary gland infection of milch cow. The bactericide can be carried by a carrier, then an antiseptic wet towel is obtained. The antiseptic wet towel could kill all bacteria causing mammary gland infection of milch cow. The killing rate for bacillus coli, staphylococcus aureus and the like, is above 99%.
Description
Technical field
The present invention relates to a kind of biocide preparation and sterilization material, relate in particular to a kind of preparation and bactericidal wet tissue of killing the bacterium that causes that cow mammary gland infects.
Background technology
Cow mammary gland infects and is mainly caused by staphylococcus aureus, Escherichia coli, streptococcus, Pseudomonas aeruginosa etc., and the drove investigation shows that the cow of the U.S. 30% to 70% has an infected breast district at least.Cow mammary gland infects the mastitis that causes, and makes 0.5 kilogram of milk cow milk yield every day loss, and the milk yield of minimizing is estimated as the 69%-80% of mastitis total losses.The direct losses that U.S.'s dairy every year causes because of mastitis are above 2,000,000,000 dollars.As seen, mastitis is huge to the economic impact of dairy industry.
Often treating cow mammary gland with the antibiotic (as penicillin, streptomycin, gentamicin, chloramphenicol, neomycin etc.) of heavy dose both at home and abroad infects, though antibiotic has certain curative effect, but because above-mentioned pathogenic bacteria have mostly produced endurance strain, therefore, curative effect is also not obvious.Cause antibiotic residue in the milk on the contrary, it is serious that the milk that these oxen produced is sneaked into the consequence that edible milk causes.FAO (Food and Agriculture Organization of the United Nation) (FAO) and The World Health Organization (WHO) all advise, the milk of extruding at least 3 days after milk cow is accepted the antibiotic therapy drug withdrawal is not directly as edible milk material (preferably taking drug withdrawal 5-7 days).And tackle mastitis at present in the world except using antibiotic not to have its medicine.
U.S. Pat 5762948 discloses the wet tissue of a kind of prevention and the infection of treatment cow mammary gland, and its composition is aided with other antimicrobial components such as alcohol based on a kind of antibacterial peptide nisin.This wet tissue can be removed the various pathogenic bacteria that cause intramammary infection, but to the DeGrain of drug resistance staphylococcus aureus, and simultaneously because the synthesis technique complexity of antibacterial peptide, the production cost height is difficult to apply.
Summary of the invention
One of technical issues that need to address of the present invention provide a kind of preparation of killing the bacterium that causes that cow mammary gland infects, to overcome the above-mentioned defective that prior art exists;
Two of the technical issues that need to address of the present invention provide a kind of bactericidal wet tissue of killing the bacterium that causes that cow mammary gland infects.
Three of the technical issues that need to address of the present invention provide a kind of preparation method of bactericidal wet tissue.
Another technical problem that the present invention need solve discloses the application of described bactericidal wet tissue.
Inventive concept of the present invention is such:
Staphylococcus lysozyme (1ysostaphin) is a kind of metalloproteinases that contains zinc, the glycine peptide bond of single-minded degradation of cell wall, the cell wall of gram-positive bacterias such as energy cracking staphylococcus aureus, thereby play the purpose of thorough killing bacteria, be difficult for to produce drug resistance, especially the effect of the pathogenic bacteria that drug resistance staphylococcus aureus etc. is difficult to eradicate is very good.But staphylococcus lysozyme is only effective to gram-positive bacteria, and cause that bacterium that cow mammary gland infects is except that gram-positive bacteria, Gram-negative bacteria has also accounted for certain ratio, as Escherichia coli etc., so that the inventor carries out lysozyme and staphylococcus lysozyme is composite, the β-1 that lysozyme can the cracking bacterial cell wall, 4 glycosidic bonds, gram-positive bacteria and Gram-negative bacteria are all had killing action, and lysozyme can also be strengthened the bactericidal action of lysostaphin except having widened fungicidal spectrum simultaneously.Two kinds of enzymes all belong to protein, do not stimulate also can not produce any toxic and side effect, because production cost is low, the charge is small simultaneously.
Eliminate and the control cow mammary gland infects effectively that way is to kill the various bacteriums that cause infection, the various pathogenic bacteria in breast district particularly not only cause and self intramammary infection also can infect the milk cow to other simultaneously.The inventor is through test of many times, staphylococcus lysozyme and lysozyme is composite by a certain percentage, and the bactericidal wet tissue of making as raw material, the various pathogenic bacteria of killing milk cow's milk district that can be simple and effective, eliminate to infect, can also play the effect of recurring and preventing of preventing simultaneously.
Technical scheme of the present invention is such:
A kind of biocide preparation comprises staphylococcus lysozyme 0.0001～0.1wt%, lysozyme 10～50wt%, stabilizing agent 1～15wt%, surfactant 0.1～0.5wt%, water surplus.
The stabilizing agent of being addressed comprises a kind of in polyethylene glycol or the shitosan, and it mainly acts on is to keep the stability of enzyme under aqueous solution state, promptly keeps the activity of enzyme.
The surfactant of being addressed comprises non-ionic surface active agent or zwitterionic surfactant, 20 〉=HLB 〉=6 preferably, as polysorbate series, comprise polysorbate20, polysorbate40, polysorbate60 or polysorbate80 etc. and betain etc., it mainly acts on is the dirt of removing milk cow's milk district, make the active component in the preparation fully act on skin surface, help absorbing.
Above-mentioned biocide preparation can be carried on the carrier, make a kind of bactericidal wet tissue, carrier comprises non-woven material or cotton gauze.
The preparation method of the bactericidal wet tissue of being addressed comprises the steps:
1, take by weighing staphylococcus lysozyme, lysozyme according to the above ratio, stabilizing agent and surfactant mix with water;
2, with the solution spraying for preparing on the carrier of required specification or with carrier impregnation in solution, take out, promptly obtain bactericidal wet tissue of the present invention.
Preparation of the present invention and bactericidal wet tissue are used to kill the various bacteriums that cause that cow mammary gland infects, and comprise Gram-negative bacteria and gram-positive bacteria.Particularly Escherichia coli, staphylococcus aureus, Streptococcusagalactiae, streptococcus dysgalactiae, streptococcus uberis and corynebacterium pyogenes.Can treat by these bacterial various mastitiss, also can prevent the generation of mastitis.
Preparation of the present invention and wet tissue forward and backwardly can be carried out disinfection by smearing or wiping milk cow's milk district giving milk, guarantee the cleaning in breast district, reduce possibility of infection.
Preparation of the present invention or bactericidal wet tissue can be smeared or be covered in the breast district that the generation of milk cow is infected, its dosage can be according to age, the state of an illness decision of milk cow, is generally twice of every day.
Bactericidal wet tissue is tested by extracorporeal disinfecting, prove its The main pathogenic fungi to causing that cow mammary gland infects: the bactericidal effect of Escherichia coli, staphylococcus aureus, Streptococcusagalactiae, streptococcus dysgalactiae, streptococcus uberis, corynebacterium pyogenes is very good, and killing rate is all above 99%.
Bactericidal wet tissue shows that to the The acute toxicity tests of Kunming small white mouse the animal via intramuscular injection is after 7 days, and no abnormal reaction, activity are normally.Observed through 7 days, 60 small white mouses all survive.The proof free of toxic effects.
Bactericidal wet tissue is to the test of SD rat vagina mucosa irritation, and standards of grading scoring and skin irritatin strength grading with reference to skin irritatin response intensity in the disinfection technology standard (third edition) 1999.11 carry out the evaluation to the vaginal mucosa stimulus intensity.The result shows that bactericidal wet tissue is 0 to the highest total mark average of rat vagina mucosa irritation reaction, belongs to nonirritant.
Bactericidal wet tissue is to the therapeutic test of breast district acute infection, and in contrast with scotcil and streptomycin, after the administration 7 days, the result shows, bactericidal wet tissue can effectively be killed the various pathogens that cause intramammary infection, especially effective especially to staphylococcus aureus, two kinds of antibiotic then can not effectively be removed the pathogen in breast district.
The test of bactericidal wet tissue prevention intramammary infection, as can be seen, the bactericidal wet tissue cow's milk district that can effectively prevent to suckle is subjected to bacterial infection, can keep the cleaning in breast district.
Embodiment
Embodiment 1
The preparation of bactericidal wet tissue
1. preparation 100g is used for the solution of bactericidal wet tissue, staphylococcus lysozyme content 0.001g (0.001wt%) wherein, lysozyme content 20g (20wt%), polyethyleneglycol content 3g (3%), poly-Mountain of Pear alcohol ester 20 content 0.2g (0.2wt%).
2. to desktop, instrument, vessel, mixer sterilization.
3. on electronic balance, accurately take by weighing staphylococcus lysozyme, lysozyme, polyethylene glycol and poly-Mountain of Pear alcohol ester 20, place mixer, add water 76.80g in proportion, stir, and be heated to 60 ℃ of dissolvings, be required solution.
4. nonwoven is cut into 8cm * 8cm, after 20 minutes, immerses in the above-mentioned solution, pull out after waiting to soak into, drip and go redundant solution, can use through ultraviolet disinfection.
Embodiment 2
The test of bactericidal wet tissue extracorporeal disinfecting
1, the preparation of test specimen:
(1) bactericidal wet tissue sample: press the method preparation of embodiment 1, staphylococcus lysozyme content 0.001wt% wherein, lysozyme content 20wt%, polyethyleneglycol content 3%, poly-Mountain of Pear alcohol ester 20 content 0.2wt%.
(2) contrast wet tissue: wherein polyethyleneglycol content 3%, poly-Mountain of Pear alcohol ester 20 content 0.2wt%.
2, test strain: staphylococcus aureus, Streptococcusagalactiae, streptococcus dysgalactiae, streptococcus uberis, Escherichia coli and corynebacterium pyogenes (above bacterial strain comes from the military region, Shenyang army horse study on prevention institute).
3, test procedure:
(1) cultured test strain is washed fresh inclined-plane with 1% peptone PBS, and be diluted to suitable concentration, 10
7～10
8Cfu/ml.
(2) sample wet tissue and contrast wet tissue are made 1 * 3cm size, and be placed in the aseptic plate.
(3) get 20ul bacterium drop and be added on the wet tissue, smear evenly and pick up counting simultaneously.
(4) the wet tissue input is contained in the test tube of 5ml neutralizer with aseptic nipper after affacting the scheduled time, mixing fully vibrates.
(5) the contrast wet tissue drops into the neutralizer test tube, fully does suitable dilution after the vibration.
(6) get whole liquid 0.5ml and carry out pour plate, 37 constant temperature are inverted and were cultivated 48 hours.
(7) calculate contrast and reclaim bacterium amount and sample recovery bacterium amount, draw killing rate.
4, result of the test
Table 1: bactericidal wet tissue extracorporeal disinfecting test killing rate result
Test strain bactericidal wet tissue contrast wet tissue
Escherichia coli 99.92% 0
Staphylococcus aureus 99.98% 0
Streptococcusagalactiae 99.92% 0
Streptococcus dysgalactiae 99.97% 0
Streptococcus uberis 99.56% 0
Corynebacterium pyogenes 99.46% 0
Embodiment 3
The test of bactericidal wet tissue extracorporeal disinfecting
1, the preparation of test specimen:
(1) bactericidal wet tissue sample: press the method preparation of embodiment 1, staphylococcus lysozyme content 0.001wt% wherein, lysozyme content 20wt%, polyethyleneglycol content 3%, beet alkali content 0.2wt%.
(2) contrast wet tissue: wherein polyethyleneglycol content 3%, beet alkali content 0.2wt%.
2, test strain: staphylococcus aureus, Streptococcusagalactiae, streptococcus dysgalactiae, streptococcus uberis, Escherichia coli and corynebacterium pyogenes (above bacterial strain comes from the military region, Shenyang army horse study on prevention institute).
3, test procedure: undertaken by embodiment 2.
4, result of the test
Table 2: bactericidal wet tissue extracorporeal disinfecting test killing rate result
Test strain bactericidal wet tissue contrast wet tissue
Escherichia coli 99.95% 0
Staphylococcus aureus 99.97% 0
Streptococcusagalactiae 99.95% 0
Streptococcus dysgalactiae 99.98% 0
Streptococcus uberis 99.62% 0
Corynebacterium pyogenes 99.67% 0
Acute toxicity experience in embodiment 4 animal bodies
1, the preparation of sample: press the required solution of method preparation bactericidal wet tissue among the embodiment 1, wherein: staphylococcus lysozyme content 0.01wt% wherein, lysozyme content 30wt%, polyethyleneglycol content 5%, poly-Mountain of Pear alcohol ester 20 content 0.3wt%.
2, experimental animal: adopt 60 of Kunming small white mouses, male and female half and half, body weight 33.5 ± 0.25g is provided by laboratory animal portion of Fudan University.
3, test method: observed animal toxic reaction under maximum dose through the intramuscular injection small white mouse in continuous once a day 7 days.Experimental result shows that the animal via intramuscular injection is after 7 days, and no abnormal reaction, activity are normally.Observed through 7 days, 60 small white mouses all survive.The proof free of toxic effects.
Embodiment 5
Bactericidal wet tissue is to experimental animal vaginal mucosa irritant test
1, sample preparation: the preparation method of bactericidal wet tissue is with reference to embodiment 1, wherein: staphylococcus lysozyme content 0.001wt% wherein, lysozyme content 20wt%, polyethyleneglycol content 3%, poly-Mountain of Pear alcohol ester 20 content 0.2wt%.
2, experimental animal: the SD rat, female, regular grade, 18, body weight 230 grams-250 grams divide three groups at random, and bactericidal wet tissue group, vehicle group and blank group, are provided by laboratory animal portion of Fudan University by 6 every group.
3, test method: with reference to 3.8 in the disinfection technology standard (third edition) 1999.11.Bactericidal wet tissue is put in the animal vagina, contacted 4 hours with vaginal mucosa.Vehicle group is soaked with sterile saline with sliver 0.01 gram and is done same test.The blank group is left intact.Put to death animal in 24 hours, take out local vagina tissue, observation has or not hyperemia, oedema phenomenon.Standards of grading scoring and skin irritatin strength grading with reference to skin irritatin response intensity in the disinfection technology standard (third edition) 1999.11 carry out the evaluation to the vaginal mucosa stimulus intensity.
4, result of the test:
Table 3: bactericidal wet tissue is to experimental animal vaginal mucosa irritant test
The irritant reaction scoring
Group number of animals (only)
Erythema oedema total points
Bactericidal wet tissue group 6000
Vehicle group 6000
Blank group 6000
Bactericidal wet tissue is 0 to the highest total mark average of rat vagina mucosa irritation reaction, belongs to nonirritant.
Embodiment 6
Bactericidal wet tissue is to the therapeutic test of breast district acute infection
1, sample is prepared
5. the preparation of bactericidal wet tissue: the preparation method presses embodiment 1, staphylococcus lysozyme content 0.008wt% wherein, lysozyme content 20wt%, polyethyleneglycol content 2%, poly-Mountain of Pear alcohol ester 20 content 0.3wt%.
Scotcil: 800,000 units, North China Pharmaceutical Factory
Streptomycin sulphate: 1,000,000 units, North China Pharmaceutical Factory
2, the preparation of experimental animal
Select lactational 4-8 year green grass or young crops, prime of life high yield, Fresian, totally 20, acute intramammary infection causes inflammation,, its symptom is mammary swelling, milk is unusual: grumeleuse is arranged, flocculus or flocculus is arranged later on.But do not have pain, do not have other symptom.Be divided into 4 groups at random, 1 group is a bactericidal wet tissue treatment group, and 2 groups are scotcil treatment group, and 3 groups are streptomycin treatment group, and 4 groups are the blank group.
3, testing program:
1 group: every day sooner or later each one inferior to after extracting breast, with bactericidal wet tissue wiping and be covered in and infect the breast district repeatedly, until skin absorbs.2,3 groups: every day, each once after extracting breast, pressed penicillin 100,000 units, the intramammary injection administration of streptomycin 100,000 units sooner or later.4 groups: control group, do not carry out any treatment, administration 7 days.
4, observation index:
(1) recovery from illness: the symptom of clinical mastitis all disappears, and the breast tissue softness does not have grumeleuse in the milk, and the color of milk is with the same white at ordinary times.
Effectively: clinical mastitis symptom alleviates, and perhaps no longer worsens, and milk is normal, but mammary swelling does not have complete obiteration.
(2) bacteriological detection: separate, cultivate, identify to treating the forward and backward pathogen that infects the breast district, wherein because cow mammary gland infects based on Escherichia coli, staphylococcus aureus, Streptococcusagalactiae, streptococcus dysgalactiae, streptococcus uberis, six kinds of bacteriums of corynebacterium pyogenes, so this detection test is based on above-mentioned six kinds of bacteriums.The concrete operations step is as follows:
A, sampling: carry out with conventional aseptic sampling, use the 10ml test tube, 5-7ml sweet milk is gathered in each breast district of every ox, attention be that preceding 2-3 abandons breast not, perform mark, the fast as far as possible 4 ℃ of refrigerators of putting into are preserved.
B, cultivation, separation: cultivate and carry out with routine, 37 ℃ of temperature, relative temperature is more than 50%.Used medium mainly contains: 7% sheep blood agar; The sheep soup agar that adds 1% aesculin; The but graceful agar in Pu; Sodium azide crystal violet blood agar; Meat stream medium etc.(above medium is from the military region, Shenyang army horse study on prevention institute)
C, evaluation
The evaluation of staphylococcus aureus: adopt test tube method and slide method to carry out, utilize citric acid rabbit plasma solidification experiments to measure.
Streptococcic evaluation: adopt the Camp test to carry out, the Camp test positive and the negative person of aesculin promptly is a Streptococcusagalactiae, the Camp test positive and the positive person of aesculin promptly is a streptococcus uberis, it promptly is streptococcus dysgalactiae that Camp tests the also negative person of negative aesculin.
Colibacillary evaluation: adopt the U.S. blue medium separation and Culture in Yihong and carry out V, P test and M, R test, V, P test negative, and M, R test positive person and be Escherichia coli.
The evaluation of corynebacterium pyogenes: adopt blood agar culture-medium to cultivate, after 36 hours, as see that the about 1-2cm of diameter is arranged, figure, projection, milky, smooth surface is glossy, and the petite person of β Qiong Xuequan occurs, is corynebacterium pyogenes.
5, result of the test
Table 4: bactericidal wet tissue is to the therapeutic test result of breast district acute infection
Bactericidal wet tissue group scotcil group streptomycin group blank group
Clinical recovery (number) 4210
Performance is (number) 1220 effectively
Escherichia coli 99.7 97.2 92.3 0
Cause of disease staphylococcus aureus 99.8 65.3 41.2 0
Bacterium kills Streptococcusagalactiae 99.5 86.2 85.3 0
The rate of going out streptococcus dysgalactiae 99.3 78.2 86.4 0
(%) streptococcus uberis 99.1 89.6 91.3 0
Corynebacterium pyogenes 99.4 78.5 87.9 0
As can be seen from the above table, bactericidal wet tissue can effectively be killed the various pathogens that cause intramammary infection, and is especially effective especially to staphylococcus aureus, and two kinds of antibiotic then can not effectively be removed the pathogen in breast district.
Embodiment 7
The test of bactericidal wet tissue prevention intramammary infection
1, sample is prepared
The preparation of bactericidal wet tissue: the preparation method presses embodiment 1, staphylococcus lysozyme content 0.001wt% wherein, lysozyme content 20wt%, polyethyleneglycol content 3%, poly-Mountain of Pear alcohol ester 20 content 0.2wt%.
Iodophor solution: 100ml, Zhejiang Agricultural Univ.
Sample solution: 1% peptone, 0.5% sodium thiosulfate, 2% Tween-80, the PBS of 0.5% lecithin, every pipe 10ml.
2, the preparation of experimental animal
Select lactational 4-8 year green grass or young crops, prime of life high yield, Fresian, normally do not have any infection symptoms, totally 3,1 group is a bactericidal wet tissue prevention group, and 2 groups are the iodophor group, and 3 groups are the blank group.
3, test method:
An aseptic cotton is wiped away the adhesional wetting sample solution adopt the breast district, detect the natural clump count of each sample of breast district.Behind the contiguous position of 1 group with the former sampling of bactericidal wet tissue wiping, 1 minute, 30 minutes, 2 hours, respectively adopt sample one time; 2 little group of methods are with 1 group, residual natural bacteria number after the detection effect, the natural bacteria killing rate before and after the calculating effect.
4, result of the test
Table 5: the result of the test that bactericidal wet tissue prevention breast district infects
Killing rate (%) bactericidal wet tissue group iodophor group blank group
After 1 minute 99.9 94.2 13.5
After 30 minutes 99.2 92.1 15.2
After 2 hours 98.4 62.3 14.6
From above-mentioned test as can be seen, the bactericidal wet tissue cow's milk district that can effectively prevent to suckle is subjected to bacterial infection, can keep the cleaning in breast district.
Claims (14)
1. a biocide preparation is characterized in that comprising staphylococcus lysozyme 0.0001～0.1wt%, lysozyme 10～50wt%, stabilizing agent 1～15wt%, surfactant 0.1～0.5wt%, water surplus.
2. biocide preparation according to claim 1 is characterized in that the stabilizing agent of being addressed is a kind of in polyethylene glycol or the shitosan.
3. biocide preparation according to claim 1 is characterized in that glass or plastic containers or the zwitterionic surfactant addressed.
4. biocide preparation according to claim 3 is characterized in that the non-ionic surface active agent of being addressed is the non-ionic surface active agent of 20 〉=hydrophilic lipophilic balance 〉=6.
5. preparation according to claim 4 is characterized in that non-ionic surface active agent is a polysorbate series.
6. biocide preparation according to claim 3 is characterized in that the zwitterionic surfactant of being addressed is a betain.
7. a bactericidal wet tissue is characterized in that being made up of carrier and the arbitrary described bactericide of load claim 1～6 thereon.
8. according to the bactericidal wet tissue of stating of claim 7, it is characterized in that described carrier comprises non-woven material or cotton gauze.
9. the preparation method of bactericidal wet tissue according to claim 7 is characterized in that comprising according to step down:
(1) take by weighing staphylococcus lysozyme, lysozyme in proportion, stabilizing agent and surfactant mix with water;
(2) with the solution spraying for preparing on the carrier of required specification or with carrier impregnation in solution, take out, promptly obtain bactericidal wet tissue of the present invention.
10. claim 7 or the 8 described bactericidal wet tissues application in killing the bacterium that causes the cow mammary gland infection.
11. the application of bactericidal wet tissue according to claim 10 is characterized in that described bacterium is Gram-negative bacteria and gram-positive bacteria.
12. the application of bactericidal wet tissue according to claim 11 is characterized in that described bacterium comprises Escherichia coli, staphylococcus aureus, Streptococcusagalactiae, streptococcus dysgalactiae, streptococcus uberis and corynebacterium pyogenes.
13., it is characterized in that in the forward and backward wiping milk cow's milk district of giving milk according to the application of the arbitrary described bactericidal wet tissue of claim 9-11.
14., it is characterized in that wiping or be covered in the breast district that the generation of milk cow is infected according to the application of the arbitrary described bactericidal wet tissue of claim 9-11.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02137243 CN1209972C (en) | 2002-09-28 | 2002-09-28 | Sterilization formulation and wet tissue, preparation method and use |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02137243 CN1209972C (en) | 2002-09-28 | 2002-09-28 | Sterilization formulation and wet tissue, preparation method and use |
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| Publication Number | Publication Date |
|---|---|
| CN1484959A true CN1484959A (en) | 2004-03-31 |
| CN1209972C CN1209972C (en) | 2005-07-13 |
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|---|---|---|---|
| CN 02137243 Expired - Lifetime CN1209972C (en) | 2002-09-28 | 2002-09-28 | Sterilization formulation and wet tissue, preparation method and use |
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| CN102575207A (en) * | 2009-08-21 | 2012-07-11 | 约翰逊父子公司 | Water-activated "green" cleaning wipe |
| CN1596991B (en) * | 2004-07-06 | 2012-08-08 | 上海高科生物工程有限公司 | Sterilization bamboo fiber gauze and its preparation method and application |
| CN103861092A (en) * | 2014-02-26 | 2014-06-18 | 金红叶纸业集团有限公司 | Skin care composition, paper towel and preparation method of paper towel |
| CN105662909A (en) * | 2016-01-29 | 2016-06-15 | 江苏天佑医用科技有限公司 | Wiping wet tissue for dairy cows and preparation method of wiping wet tissue |
| CN106087419A (en) * | 2016-07-04 | 2016-11-09 | 安徽省曾氏保洁品有限公司 | A kind of cotton mop treatment fluid and its preparation method and application |
| CN108014032A (en) * | 2018-01-02 | 2018-05-11 | 深圳市芭格美生物科技有限公司 | A kind of hip pad wet tissue and preparation method thereof |
| CN108125797A (en) * | 2018-01-02 | 2018-06-08 | 深圳市芭格美生物科技有限公司 | A kind of hand mouth wet tissue and preparation method thereof |
| CN108186403A (en) * | 2018-01-02 | 2018-06-22 | 深圳市芭格美生物科技有限公司 | A kind of impregnated wipe and preparation method thereof |
| CN111749023A (en) * | 2019-03-28 | 2020-10-09 | 深圳市芭格美生物科技有限公司 | Lysozyme non-woven fabric and preparation method thereof |
| CN111764174A (en) * | 2019-04-01 | 2020-10-13 | 深圳市芭格美生物科技有限公司 | Non-woven fabric containing enzyme and preparation method thereof |
| CN113336602A (en) * | 2021-07-28 | 2021-09-03 | 广西易邦科技有限公司 | Novel fertilizer containing lysozyme |
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2002
- 2002-09-28 CN CN 02137243 patent/CN1209972C/en not_active Expired - Lifetime
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1596991B (en) * | 2004-07-06 | 2012-08-08 | 上海高科生物工程有限公司 | Sterilization bamboo fiber gauze and its preparation method and application |
| CN102575207A (en) * | 2009-08-21 | 2012-07-11 | 约翰逊父子公司 | Water-activated "green" cleaning wipe |
| CN103861092A (en) * | 2014-02-26 | 2014-06-18 | 金红叶纸业集团有限公司 | Skin care composition, paper towel and preparation method of paper towel |
| CN103861092B (en) * | 2014-02-26 | 2016-07-13 | 金红叶纸业集团有限公司 | A kind of skin care compositions, napkin and preparation method thereof |
| CN105662909A (en) * | 2016-01-29 | 2016-06-15 | 江苏天佑医用科技有限公司 | Wiping wet tissue for dairy cows and preparation method of wiping wet tissue |
| CN106087419A (en) * | 2016-07-04 | 2016-11-09 | 安徽省曾氏保洁品有限公司 | A kind of cotton mop treatment fluid and its preparation method and application |
| CN108014032A (en) * | 2018-01-02 | 2018-05-11 | 深圳市芭格美生物科技有限公司 | A kind of hip pad wet tissue and preparation method thereof |
| CN108125797A (en) * | 2018-01-02 | 2018-06-08 | 深圳市芭格美生物科技有限公司 | A kind of hand mouth wet tissue and preparation method thereof |
| CN108186403A (en) * | 2018-01-02 | 2018-06-22 | 深圳市芭格美生物科技有限公司 | A kind of impregnated wipe and preparation method thereof |
| CN111749023A (en) * | 2019-03-28 | 2020-10-09 | 深圳市芭格美生物科技有限公司 | Lysozyme non-woven fabric and preparation method thereof |
| CN111749023B (en) * | 2019-03-28 | 2023-01-24 | 深圳市芭格美生物科技有限公司 | Lysozyme non-woven fabric and preparation method thereof |
| CN111764174A (en) * | 2019-04-01 | 2020-10-13 | 深圳市芭格美生物科技有限公司 | Non-woven fabric containing enzyme and preparation method thereof |
| CN113336602A (en) * | 2021-07-28 | 2021-09-03 | 广西易邦科技有限公司 | Novel fertilizer containing lysozyme |
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|---|---|
| CN1209972C (en) | 2005-07-13 |
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