CN1483319A - Acquired antiviral inductive agent of compound oligose plant system and preparation thereof - Google Patents
Acquired antiviral inductive agent of compound oligose plant system and preparation thereof Download PDFInfo
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- CN1483319A CN1483319A CNA02130937XA CN02130937A CN1483319A CN 1483319 A CN1483319 A CN 1483319A CN A02130937X A CNA02130937X A CN A02130937XA CN 02130937 A CN02130937 A CN 02130937A CN 1483319 A CN1483319 A CN 1483319A
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Abstract
The present invention relates to a new type plant protection product capable of making crops obtain resistance to disease and its preparation method. Its key is characterized by combining some active oligosaccharide together with a kind of specific plant signal transducing factor to form a kind of acquired disease-resisting inducing agent with higher activity for plant system.
Description
This patent is the novel plant protection product and the preparation thereof that can make crops obtain resistance against diseases about a kind of, the key of this patent is that several active oligosaccharides and a kind of specific plant signal transduction molecule are combined, and forms the acquired disease-resistant derivant of the higher botanical system of a class activity.
Botanical system obtains resistance, and (systemic acquired resistance is to be infected or after some biochemical preparation induces processing by downright bad type pathogen SAR), and plant is infected or into treatment sites produces the resistance that pathogen is subsequently infected.This resistance has the 3 big characteristics that other resistances form that are different from: the one, and systematicness, promptly SAR can show the non-inducible factor into treatment sites of plant; The 2nd, persistence, promptly SAR keeps several weeks even some months after producing; The 3rd, broad spectrum activity, promptly SAR produces inhibitory action to disease due to fungi, bacterium, the virus simultaneously.Can excite the chemical substance of making deposits yields SAR to be known as crop activator or derivant (Elicitor), it is representing a kind of brand-new chemistry in agricultural chemicals, more representing a kind of brand-new plant protection technology and notion, changed the direct sterilization principle that traditional bactericide is all followed, at the beginning of the release, promptly be described as " change of dynasties " and have the novel pesticide of changing meaning.
The molecular biology basis that botanical system obtains resistance be plant with the interactional long process of pathogene in, various and opposing pathogene relevant material have been formed by various metabolic pathways, as lignin, protective plant protecting agent, active oxygen etc., these materials are that itself exists in the original plant corpus a bit, and some is to induce formation behind the pathogenic infection.Is pathogene the resistance system that how to start plant so? studies show that, the active ingredient of energy activated plant defensive avionics system is pathogen cells ancient piece of jade, round, flat and with a hole in its centre polysaccharide [J.K.Sharp in the pathogene, B.Valent, P.Albersheim, J.Biol.Chem, 1984,259:11312.], this discovery also is that to meet carbohydrate be the so generally acknowledged modern scientific research rule of information transmitter substance in the organism.Further research discloses later on, and the highest active position is the oligose fragment that special construction is arranged that constitutes polysaccharide in the polysaccharide.Oligose fragment can be used as early stage informational molecule in plant, to the disease-resistant bacterium of plant infect, grow, morphogenesis etc. has significant impact.In disease-resistant bacterium infection processs, oligosaccharides can excite the multiple defense reaction that comprises that protective plant protecting agent synthesizes.
Oligosaccharides can be [the J.K.Sharp that U.S. Albersheim found in 84 years as the activator (Elicitor) of plant ego defense system, B.Valent, P.Albersheim, J.Biol.Chem, 1984,259:11321.] the oligosaccharides activator is that a class can be at the molecule of the special metabolism of DNA transcriptional level adjusted, gene expression.Grape seven sugar that β in the cell wall polysaccharides of fungal pathogens Pmg (Phytophthora megasperma f.sp.glycinea) connects are found first oligosaccharides activator, and 10ng (1 * 10
-8Gram) be applied to 1 gram plant tissue and can produce enough protective plant protecting agents, be equivalent to 1 gram oligosaccharides activator and can make the tissue of 1000 tons of crops produce the protective plant protecting agent of q.s, up to now it be found in the protective plant protecting agent activator most effective.Studies show that afterwards, the methyl of six sugar of the few glucose unit of reducing end and pi-allyl glycosides have the function that is similar to seven sugar (Cote F, Hahn M, Plant Mol.Biol.1994,26:1379.).These discoveries have caused organic chemist's great interest, the study on the synthesis of oligosaccharides activator has all successively been carried out in several famous laboratories, but because used reagent costliness, reactions steps are various, only can be used for the checking research of structure, activity relationship, be difficult to prepared in batches.Ecological Environment Research Center, Chinese Academy of Sciences has obtained up-to-date progress to the research of oligosaccharides activator, and the synthetic method of the oligosaccharides activator of being invented is succinct, effectively (Ning Jun, Kong Fanzuo, plant ego defense system activating agent 6 sugar simple and easy synthetic.Chinese patent application number: 99119757.7; Ning Jun, Kong Fanzuo can be used as prevent and treat the disease agricultural chemicals, can make plant produce the grape oligosaccharides of protective plant protecting agent.Chinese patent application number: 00100713.0; Ning Jun, Kong Fanzuo, a kind of preparation method of six sugar, world patent application number: PC 00253).Synthetic oligosaccharides inducing plant is being produced on the basis that disease-resistant mechanism furthers investigate, after we find that a few class natural polysaccharides are handled through certain chemical technology, can obtain the composite oligosaccharide product, combine with a kind of specific plant signal transduction molecule again, can form the higher composite oligosaccharide preparation of a kind of inducing plant anti-disease activity, said preparation also has and significantly to promote plant growing, improves other critical functions such as the quality of fruit and mouthfeel.
In achieving the above object, this patent provides a class can make crops obtain the novel plant protection product of resistance against diseases, this product is that some active oligosaccharides and a kind of specific plant signal transduction molecule are combined, form the acquired disease-resistant derivant of the higher botanical system of a class activity, directly act on the crops that growing, thereby make crops obtain the ability of opposing disease.
Described active oligosaccharides mainly comprises ligoglucoside from the catabolite of natural polysaccharide, amino ligoglucoside and acetylamino ligoglucoside or the like.
The described oligosaccharides degree of polymerization is 2-30.
Described plant signal transduction molecule mainly is a salicylic acid, acetylsalicylic acid and higher fatty acid etc.
Described composite oligosaccharide preparation can be a solid, also can be certain density aqua, if liquid, the concentration of oligosaccharides is 0.5-5%, and salicylic concentration is 0.01-1%.
Described active oligosaccharides and a kind of specific plant signal transduction molecule such as salicylic acid are to combine by the chemical bonding effect, form the oligosaccharides compound.
Specify this product with example below, the preparation of product and on crops, prevent and treat the effect of disease.
Example 1. obtains amino grape oligosaccharides that the degree of polymerization is 2-30 and salicylic acid with shitosan through chemical degradation and is mixed with 2% composite oligosaccharide solution, and wherein salicylic content is 0.2%.Directly be sprayed onto by on the leaf of 45 centimetres of the ash arrhizus bacteria low-grade infection high tomatoes behind 200 times of its dilute with waters, control group for only spray clear water by the leaf of ash arrhizus bacteria low-grade infection, by the ratio of metering experimental group, determine anti-effect of curing the disease after three days with the lesion area of control group.The result is as follows:
The result of the anti-tomato gray mold of described composite oligosaccharide
Disease index (%) relative control effect (%)
Ash arrhizus bacteria infects clear water control group 98.6 0
Composite oligosaccharide solution 20.1 76.2
Ten thousand mould clever experimental group (500ppm) 18.3 81.4
200 times of composite oligosaccharide liquid directly spray the tomato of catching an illness as seen from the above table, can reach the effect of well preventing and treating gray mold, and are suitable with chemical pesticide ten thousand mould spirits.
Example 2. is with example 1 gained composite oligosaccharide solution, directly be sprayed onto behind 200 times of the dilute with waters by Huang and wither on the leaf of cotton of bacterium low-grade infection, control group is the leaf that only sprays the bacterium low-grade infection of being withered by Huang of clear water, by the ratio of metering experimental group, determine anti-effect of curing the disease after three days with the lesion area of control group.The result is as follows:
The wither result of bacterium disease of the anti-cotton Huang of described composite oligosaccharide
Disease index (%) relative control effect (%)
The Huang bacterium that withers infects clear water control group 97.9 0
Composite oligosaccharide solution 21.3 78.1
Carbendazim experimental group (400ppm) 19.1 81.2
200 times of composite oligosaccharide liquid directly spray the cottons that catch an illness as seen from the above table, can reach the well anti-effect of bacterium disease of withering of harnessing the Yellow River, and are suitable with the chemical pesticide carbendazim.
Example 3. is with the composite oligosaccharide solution of example 1 gained, directly be sprayed onto by on the leaf of the capsicum of anthrax bacteria low-grade infection behind 200 times of the dilute with waters, control group is for only to spray clear water by the leaf of anthracnose low-grade infection, by the ratio of metering experimental group, determine anti-effect of curing the disease after three days with the lesion area of control group.The result is as follows:
The result of the anti-pepper anthracnose of described composite oligosaccharide
Disease index (%) relative control effect (%)
Anthracnose infects clear water control group 90.2 0
Composite oligosaccharide liquid 20.3 79.6
Carbendazim experimental group (200ppm) 20.0 82.2
Composite oligosaccharide liquid directly sprays the capsicum of dying anthracnose as seen from the above table, can reach the effect of good control anthrax bacteria, and is suitable with the chemical pesticide carbendazim.
Claims (6)
1. some active oligosaccharides and a kind of specific plant signal transduction molecule combine, and form the acquired disease-resistant derivant of the higher botanical system of a class activity.
2. according to claim 1, described active oligosaccharides mainly comprises ligoglucoside from the catabolite of natural polysaccharide, amino ligoglucoside and acetylamino ligoglucoside or the like.
3. according to claim 1, the described oligosaccharides degree of polymerization is 2-30.
4. according to claim 1, described plant signal transduction molecule mainly is a salicylic acid, acetylsalicylic acid and higher fatty acid etc.
5. according to claim 1, described composite oligosaccharide preparation can be a solid, also can be certain density aqua, if liquid, the concentration of oligosaccharides is 0.5-5%, and salicylic concentration is 0.01-1%.
6. according to claim 1, active oligosaccharides and a kind of specific plant signal transduction molecule such as salicylic acid are to combine by the chemical bonding effect, form the oligosaccharides compound.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA02130937XA CN1483319A (en) | 2002-09-19 | 2002-09-19 | Acquired antiviral inductive agent of compound oligose plant system and preparation thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA02130937XA CN1483319A (en) | 2002-09-19 | 2002-09-19 | Acquired antiviral inductive agent of compound oligose plant system and preparation thereof |
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| CN1483319A true CN1483319A (en) | 2004-03-24 |
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| CNA02130937XA Pending CN1483319A (en) | 2002-09-19 | 2002-09-19 | Acquired antiviral inductive agent of compound oligose plant system and preparation thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103449892A (en) * | 2013-01-24 | 2013-12-18 | 杜新明 | Novel plant vaccine |
| CN113993380A (en) * | 2019-06-17 | 2022-01-28 | 昭和电工株式会社 | Plant vigor agent comprising exogenous elicitor and endogenous elicitor, and use thereof |
-
2002
- 2002-09-19 CN CNA02130937XA patent/CN1483319A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103449892A (en) * | 2013-01-24 | 2013-12-18 | 杜新明 | Novel plant vaccine |
| CN113993380A (en) * | 2019-06-17 | 2022-01-28 | 昭和电工株式会社 | Plant vigor agent comprising exogenous elicitor and endogenous elicitor, and use thereof |
| CN113993380B (en) * | 2019-06-17 | 2023-12-22 | 株式会社力森诺科 | Plant vigor agent comprising exogenous and endogenous elicitors, and uses thereof |
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