CN1481675A - Minitype quick breeding method for 'Huaye' japan buddleia - Google Patents
Minitype quick breeding method for 'Huaye' japan buddleia Download PDFInfo
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- CN1481675A CN1481675A CNA031178103A CN03117810A CN1481675A CN 1481675 A CN1481675 A CN 1481675A CN A031178103 A CNA031178103 A CN A031178103A CN 03117810 A CN03117810 A CN 03117810A CN 1481675 A CN1481675 A CN 1481675A
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Abstract
The mini fast propagation process of Buddleja japonica 'Variegata' includes the steps of starting culture, proliferation culture, rooting culture and transplantation. The present invention has theoretical and practical significance in the research of genetic stability of leaf spot plant variety and the extracorporeal maintenance of the variety resource and lays down the foundation for the commercial production of Buddleja japonica 'Variegata'.
Description
Technical field: the present invention relates to the miniature quick-breeding method of a plant species, particularly, relate to miniature quick-breeding method a kind of 1, ' floral leaf ' Japanese Lindley Butterflybush Herb (Buddleja japonica ' Variegata ').
Technical background: Japanese floral leaf Lindley Butterflybush Herb (Buddleja japonica ' Variegata ') is the evergreen undershrub that the Loganiaceae Lindley Butterflybush Herb belongs to, and is a kind of new varieties of viewing and admiring of suit potted plant or flower bed layout.Its strain shape grace, flourishing, the leaf margin golden yellow, the leaf central part bottle green has ornamental value.Both at home and abroad to the part Lindley Butterflybush Herb belong to view and admire kind and kind thereof nourishing and generating and seminal propagation is studied, but Shang Weijian carries out miniature fast numerous report to this kind.
Summary of the invention: the present invention is directed to prior art and Japanese floral leaf Lindley Butterflybush Herb (Buddlejajaponica ' Variegata ') is not carried out miniature fast numerous research, stem apex or stem section with ' floral leaf ' Japanese Lindley Butterflybush Herb are explant, have realized miniature fast numerous purpose of its floral leaf feature stability heredity.In order to realize purpose of the present invention, the invention provides following technical scheme:
The miniature quick-breeding method of ' floral leaf ' Japanese Lindley Butterflybush Herb (Buddleja japonica ' Variegata ') comprises starting cultivation, enrichment culture, culture of rootage, transplant step that condition of culture selects to start medium: MS+6-BA0.05mgL
-1(unit down together)+IBA0.05+NAA0.05; Proliferated culture medium: 1. MS+6-BA0.1+NAA0.1; 2. MS+6-BA0.2+NAA0.1; 3. MS+6-BA0.5+NAA0.1; Root media: MS+IAA0.1+IBA0.05, above medium all add 3% sucrose, 0.65% agar, PH5.8, and cultivation temperature is (23 ± 1) ℃, light application time 12hd
-1, illuminance 1000～1500lx, start cultivating is that the stem section that cuts terminal bud and band axillalry bud is at first cleaned with soap and running water, then with distilled water flushing clean after, on super-clean bench,, use 0.1% HgCl again with 70% alcohol disinfecting 30s
2Solution disinfection 3～5min, aseptic water washing 5～6 times is inoculated on the startup medium, and through the cultivation of 10～15d, stem segment with axillary buds begins to sprout, and stem apex obviously extends, and the 30d bud can grow up to the young sprout of 3～5cm, does not have the bud of growing thickly and produces; Enrichment culture be the aseptic seedling that will start to cultivate obtain under aseptic condition, remove radical leaves, be cut into the stem section or the stem apex that contain 1 axillalry bud about long 1cm, be seeded in proliferated culture medium respectively and 1. 2. 3. go up; Culture of rootage is the clump bud that enrichment culture is produced, choose the long terminal bud of 2-3cm and be inoculated in upward root induction of root media MS+IAA0.1+IBA0.05, transplanting is when adventive root grows to 0.5～1cm, do not take out seedling after in the laboratory, not opening bottle cap hardening 2～3d under the natural lighting condition, the medium of careful flush away root, soak 15min with 1: 1000 carbendazim solution, planting in the greenhouse with the perlite is in the seedbed of matrix, adding a cover plastic film preserves moisture, throw off film after one week, the seedling behind two all transplant survivals can change nature laterite over to: leaf mould: carry out strong seedling culture and blooming cultivation in the mixed-matrix of perlite=4: 2: 1 (V/V).
Above-mentioned cultural method keeps ' floral leaf ' Japanese Lindley Butterflybush Herb to view and admire the miniature fast numerous at 6-BA0.1mgL of stability of characteristics heredity
-1The MS medium in continuous culture more than 10 generations.
Embodiment: come further essentiality content of the present invention to be described below in conjunction with embodiments of the invention, but content of the present invention is not limited thereto.
Embodiment 1:
The Japanese Lindley Butterflybush Herb of 1 botanical name ' floral leaf ' (Buddlejajaponica ' Variegata ').
The stem section of 2 material classification stem apexs and band axillalry bud.
3 condition of culture start medium: MS+6-BA0.05mgL
-1(unit down together)+IBA0.05+NAA0.05; Proliferated culture medium: 1. MS+6-BA0.1+NAA0.1; 2. MS+6-BA0.2+NAA0.1; 3. MS+6-BA0.5+NAA0.1; Root media: MS+IAA0.1+IBA0.05.Above medium all adds 3% sucrose, 0.65% agar, PH5.8.Cultivation temperature is (23 ± 1) ℃, light application time 12hd
-1, illuminance 1000～1500lx.
4 growths and differentiation situation
At first clean 4.1 start to cultivate the stem section that cuts terminal bud and band axillalry bud with soap and running water, then with distilled water flushing clean after, on super-clean bench,, use 0.1% HgCl again with 70% alcohol disinfecting 30s
2Solution disinfection 3～5min, aseptic water washing 5～6 times is inoculated on the startup medium, and through the cultivation of 10～15d, stem segment with axillary buds begins to sprout, and stem apex obviously extends, and the 30d bud can grow up to the young sprout of 3～5cm, does not have the bud of growing thickly and produces.
4.2 enrichment culture will start the aseptic seedling of cultivation acquisition under aseptic condition, remove radical leaves, be cut into the stem section or the stem apex that contain 1 axillalry bud about long 1cm, being seeded in proliferated culture medium respectively 3. 2. 1. goes up, the statistics of enrichment culture 30d shows: 1. the growth of the sprout in number medium is best, forms the bud of growing thickly, and bastem portion has few callus to form, floral leaf remains unchanged, and about 10% propagation bud can grow 1～3 adventive root.Growth coefficient can reach 4; 2. the short and small and blade of the sprout in number medium obviously diminishes, and has callus to produce, and has a spot of green seedling and etiolated seedling to occur in the bud of growing thickly of base portion, and growth coefficient is 6; 3. the sprout in number medium propagation is grown thickly, and the formation rosette-stape that has has the glass seedling about 10% to produce, and the floral leaf proterties changes, and the grow thickly green portion on budling blade face of base portion reduces, and there is the symptom that is tending towards yellow-white on the blade face, and the base portion callus increases.
View and admire the miniature quick-breeding method of stability of characteristics heredity 4.3 keep ' floral leaf ' Japanese Lindley Butterflybush Herb: the result of above enrichment culture shows that ' floral leaf ' proterties of this Lindley Butterflybush Herb mainly is subjected to the influence of 6-BA, and 6-BA concentration is higher than 0.1mgL
-1More than ' floral leaf ' proterties morph.Experimental result is as follows: at 6-BA0.1mgL
-1The MS medium in continuous culture more than 10 generations, the offspring still can keep ' floral leaf ' Japanese Lindley Butterflybush Herb to view and admire the genetic stability of characteristic.Its multiplication rate is 4n (n for cultivate algebraically), 1 year aseptic plant can breed 1,000,000 with maternal living offspring.
4.4 culture of rootage with the clump bud that enrichment culture produces, is chosen the long terminal bud of 2 ~ 3cm and is inoculated in root media: the last root induction of MS+IAA0.1+IBA0.05.12d left and right sides sprout begins to take root, and every budling can take root 4～6 behind the 17d, and rooting rate reaches more than 95%.
4.5 transplant when adventive root grows to 0.5～1cm, do not take out seedling after in the laboratory, not opening bottle cap hardening 2～3d under the natural lighting condition, the medium of careful flush away root, soak 15min with 1: 1000 carbendazim solution, planting in the greenhouse with the perlite is in the seedbed of matrix, add a cover plastic film and preserve moisture, throw off film after the week, survival rate reaches 97% after two weeks.Seedling behind the transplant survival can change nature laterite over to: leaf mould: carry out strong seedling culture and blooming cultivation in the mixed-matrix of perlite=4: 2: 1 (V/V).
The present invention has theoretical realistic meaning to the steady characteristic of heredity of research tikka class fancy breed and the {in vitro} conservation of this variety source of realization, lays a good foundation for commercially producing of Japanese floral leaf Lindley Butterflybush Herb simultaneously.
Claims (2)
1, the miniature quick-breeding method of ' floral leaf ' Japanese Lindley Butterflybush Herb (Buddlija japonica ' Variegata '), comprise starting cultivation, enrichment culture, culture of rootage, transplant step, it is characterized in that the condition of culture selection starts medium: MS+6-BA0.05mgL
-1(unit down together)+IBA0.05+NAA0.05; Proliferated culture medium: 1. MS+6-BA0.1+NAA0.1; 2. MS+6-BA0.2+NAA0.1; 3. MS+6-BA0.5+NAA0.1; Root media: MS+IAA0.1+IBA0.05, above medium all add 3% sucrose, 0.65% agar, PH5.8, and cultivation temperature is (23 ± 1) ℃, light application time 12hd
-1, illuminance 1000～1500lx, start cultivating is that the stem section that cuts terminal bud and band axillalry bud is at first cleaned with soap and running water, then with distilled water flushing clean after, on super-clean bench,, use 0.1% HgCl again with 70% alcohol disinfecting 30s
2Solution disinfection 3～5min, aseptic water washing 5～6 times is inoculated on the startup medium, and through the cultivation of 10～15d, stem segment with axillary buds begins to sprout, and stem apex obviously extends, and the 30d bud can grow up to the young sprout of 3～5cm, does not have the bud of growing thickly and produces; Enrichment culture be the aseptic seedling that will start to cultivate obtain under aseptic condition, remove radical leaves, be cut into the stem section or the stem apex that contain 1 axillalry bud about long 1cm, be seeded in proliferated culture medium respectively and 1. 2. 3. go up; Culture of rootage is the clump bud that enrichment culture is produced, choose the long terminal bud of 2-3cm and be inoculated in upward root induction of root media MS+IAA0.1+IBA0.05, transplanting is when adventive root grows to 0.5～1cm, do not take out seedling after in the laboratory, not opening bottle cap hardening 2～3d under the natural lighting condition, the medium of careful flush away root, soak 15min with 1: 1000 carbendazim solution, planting in the greenhouse with the perlite is in the seedbed of matrix, adding a cover plastic film preserves moisture, throw off film after one week, the seedling behind two all transplant survivals can change nature laterite over to: leaf mould: carry out strong seedling culture and blooming cultivation in the mixed-matrix of perlite=4: 2: 1 (V/V).
2, cultural method according to claim 1 is characterized in that keeping ' floral leaf ' Japanese Lindley Butterflybush Herb to view and admire the miniature fast numerous at 6-BA0.1mgL of stability of characteristics heredity
-1The MS medium in continuous culture more than 10 generations.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB031178103A CN1194604C (en) | 2003-04-30 | 2003-04-30 | Minitype quick breeding method for 'Huaye' japan buddleia |
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| CNB031178103A CN1194604C (en) | 2003-04-30 | 2003-04-30 | Minitype quick breeding method for 'Huaye' japan buddleia |
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| CN1481675A true CN1481675A (en) | 2004-03-17 |
| CN1194604C CN1194604C (en) | 2005-03-30 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115633639A (en) * | 2022-11-02 | 2023-01-24 | 中国科学院昆明植物研究所 | Culture medium combination for tissue culture and rapid propagation of buddleja plants and application thereof |
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2003
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115633639A (en) * | 2022-11-02 | 2023-01-24 | 中国科学院昆明植物研究所 | Culture medium combination for tissue culture and rapid propagation of buddleja plants and application thereof |
| CN115633639B (en) * | 2022-11-02 | 2023-09-22 | 中国科学院昆明植物研究所 | Culture medium combination for tissue culture and rapid propagation of buddleia plants and application thereof |
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| CN1194604C (en) | 2005-03-30 |
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