CN1480391A - Method for preparing colloidal nano granules of selenium - Google Patents
Method for preparing colloidal nano granules of selenium Download PDFInfo
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- CN1480391A CN1480391A CNA021293600A CN02129360A CN1480391A CN 1480391 A CN1480391 A CN 1480391A CN A021293600 A CNA021293600 A CN A021293600A CN 02129360 A CN02129360 A CN 02129360A CN 1480391 A CN1480391 A CN 1480391A
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- Prior art keywords
- xitix
- electroselenium
- selenium
- selenous acid
- nano granules
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Abstract
A colloidal Se nanoparticle is prepared from seleninic acid and ascorbic acid at ordinary temp. It can be used as the marker in immunochromatography with high sensitivity and speed. Its advantages are short preparing period and high output.
Description
Technical field
The present invention relates to a kind of method for preparing colloidal nano granules of selenium at normal temperatures.
Background technology
Need rapid detection in many cases, as emergency treatment, on-the-spot extensive examination, on-the-spot blood test and family's self check etc.In existing many method for quickly detecting, highly sensitive, high specificity, the required time of first-elected immunochromatography are the shortest.Comparatively commonly used is colloid gold label immunochromatography detection method, and the gordian technique of this method has synthetic, the proteic mark of colloidal solid and the making treatment process of chromatographic apparatus.Colloid gold particle can be by the reduction reaction preparation of Trisodium Citrate to gold trichloride, and the size of colloid gold particle can change by the concentration of adjusting Trisodium Citrate.Also useful abroad colloid gold particle and electroselenium particle mixed mark immunochromatography detection method (WO9723774).The employed raw material of external preparation electroselenium particle is SeO
2, need under heating condition, carry out, reaction needed is about 1 hour, time is longer, and OD520 reaches 1.457, and (US5348891) yields poorly, it is lower that marker is used for the sensitivity of immunochromatography, is limited to 2.5ng/mL under the detection of detection hepatitis B surface antigen(HBsAg) (HBsAg).
Summary of the invention
The present invention is a kind of electroselenium particulate preparation method, it is characterized in that being that raw material is prepared with selenous acid and xitix at normal temperatures.Its preparation method may further comprise the steps:
(1) in reactor, adds xitix, selenous acid and xitix successively;
(2) dripped selenous acid and xitix at interval on the 4th minute from the reaction beginning, carry out spectral scan then, be advisable to occur maximum absorption band between 500-600nm;
(3) add 10% dextran termination reaction;
(4) the collection product is standby.
The raw material that the bright described electroselenium preparation method of this law adopts is a selenous acid, and reaction is carried out at normal temperatures, does not need heating.Reaction only needs 20 minutes, and output OD520 can reach more than 30, has shortened the time.It is higher to adopt the marker of the inventive method to be used for the sensitivity that immunochromatography detects, and detects under the detection of hepatitis B surface antigen(HBsAg) and is limited to 0.2ng/mL.
Electroselenium preparation method of the present invention has conveniently, saves time, highly sensitive, output is high, low 10 times of labelled protein amount ratio ordinary method consumption; Freeze Drying Technique strengthens the stability of marker greatly, but room temperature preservation is more than 1 year.
Embodiment embodiment colloid nanometer selenium synthetic method
1. reagent
3.3% selenous acid, 10% xitix, 10% Dextran T 70
2. synthesis step: with synthetic 100ml electroselenium is example.
2.1 go to select the stirrer that is fit to size for use from place clean glassware (but the silication of having ready conditions) in water 100ml, middling speed stirs.
Add xitix, selenous acid, each 1ml of xitix 2.2 be docile and obedient preface.
2.3 dripped 0.5ml selenous acid and 1ml xitix at interval from the 4th minute, repeat altogether to drip 20 times.Carry out spectral scan then, be advisable to occur maximum absorption band between 500～600nm.
2.4 add the 10% Dextran T 70 termination reaction of 1ml.
3. the processing of product: product can be preserved 2 months for 4 ℃.Embodiment two immunochromatographyassay assay HBsAg
1. antibody labeling
1.1 reagent 0.2mol/L K
2CO
3, 10%PEG20000,10%BSA, TBS preserves liquid (0.02mol/L Tris, 0.15mmol/L NaCl, 1%BSA, 0.05%NaN
3)
1.2 the processing of electroselenium
1.2.1 centrifugal 1 minute of 1000r/min removes macrobead.
1.2.2 adjusting electroselenium OD value with deionized water is 15.0.
1.2.3 with 0.2mol/L K
2CO
3Adjust electroselenium pH=7.5.
1.3 the mark of antibody
1.3.1 in 1ml electroselenium solution, add the antibody to be marked of 5～20 μ g, mixing, mark 10 minutes.
1.3.2 adding 10% BSA is 1% to final concentration, mixing was placed 10 minutes.
1.3.3 centrifugal 5 minutes of 7000r/min, the supernatant of running quickly is with TBS liquid washing 1 time.
1.3.4 use with the isopyknic TBS of former electroselenium resuspended.
2. the freeze-drying of marker is preserved
2.1 in above-mentioned resuspended liquid, add final concentration and be 0.5% casein and the trehalose of 0.15mol/L.
The thing 15 μ l/ bars 2.2 on the glassmat of 0.3 * 0.5cm size, label ,-70 ℃ freezing 30 minutes, negative pressure was drained 2 hours.
3. the bag quilt of antibody
3.1 on nitrocellulose filter, use specific antibody specking or line, 1 μ l/ bar.Placed 15 minutes for 37 ℃, then sealing.
3.2 sealing: chromatographic film is dipped in the confining liquid, and 37 ℃ were sealed 30 minutes.
3.3, each 5 minutes, use the distilled water rinsing at last five times, 37 ℃ of dryings 30 minutes with rinsing liquid rinsing twice.
3.4 4 ℃ of dry sealings are preserved.
3.5 be used to detect HBsAg: with 100 μ l serum or whole bloods, no more than 15 minutes of time spent, sensitivity can reach 0.2ng/ml.
Claims (1)
1. electroselenium particulate preparation method may further comprise the steps:
(1) in reactor, adds xitix, selenous acid and xitix successively;
(2) dripped selenous acid and xitix at interval on the 4th minute from the reaction beginning, carry out spectral scan then, be advisable to occur maximum absorption band between 500～600nm;
(3) add 10% dextran termination reaction;
(4) the collection product is standby.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA021293600A CN1480391A (en) | 2002-09-06 | 2002-09-06 | Method for preparing colloidal nano granules of selenium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA021293600A CN1480391A (en) | 2002-09-06 | 2002-09-06 | Method for preparing colloidal nano granules of selenium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1480391A true CN1480391A (en) | 2004-03-10 |
Family
ID=34144129
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA021293600A Pending CN1480391A (en) | 2002-09-06 | 2002-09-06 | Method for preparing colloidal nano granules of selenium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1480391A (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100389058C (en) * | 2005-04-19 | 2008-05-21 | 北京天天硒旺纳米生物技术有限公司 | Load type nano selenium and preparation method |
| CN1980736B (en) * | 2004-06-21 | 2010-05-05 | 约翰森·马瑟公开有限公司 | A sol comprising hybrid transition metal oxide nanoparticles |
| US20110053766A1 (en) * | 2008-02-28 | 2011-03-03 | Brown University | Nanostructured sorbent materials for capturing environmental mercury vapor |
| CN1871955B (en) * | 2005-06-01 | 2011-09-14 | 中国卫虎有限公司 | Production method of cigarette filter-tip containing nanomaterial and application of such namomaterial in manufacturing cigarette |
| US8491865B2 (en) | 2008-02-28 | 2013-07-23 | Brown University | Nanostructured sorbent materials for capturing environmental mercury vapor |
| CN104555951A (en) * | 2014-12-31 | 2015-04-29 | 烟台佳隆纳米产业有限公司 | Method for preparing and storing nano-selenium through cryogenic technology |
| CN104807999A (en) * | 2014-01-28 | 2015-07-29 | 兰州大学 | Electroselenium rapid detection test strip |
| CN107167615A (en) * | 2017-05-16 | 2017-09-15 | 河南大学 | A kind of electroselenium detected for clenobuterol hydrochloride, colloidal selenium marked thing, detection card and preparation method thereof, application |
| CN108477618A (en) * | 2018-02-05 | 2018-09-04 | 江苏大学 | A kind of controllable method for preparing of pectin nanometer selenium |
| CN113383960A (en) * | 2021-06-10 | 2021-09-14 | 广州大学 | High-stability protein polypeptide-nano selenium and preparation method and application thereof |
-
2002
- 2002-09-06 CN CNA021293600A patent/CN1480391A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1980736B (en) * | 2004-06-21 | 2010-05-05 | 约翰森·马瑟公开有限公司 | A sol comprising hybrid transition metal oxide nanoparticles |
| CN100389058C (en) * | 2005-04-19 | 2008-05-21 | 北京天天硒旺纳米生物技术有限公司 | Load type nano selenium and preparation method |
| CN1871955B (en) * | 2005-06-01 | 2011-09-14 | 中国卫虎有限公司 | Production method of cigarette filter-tip containing nanomaterial and application of such namomaterial in manufacturing cigarette |
| US9038829B2 (en) | 2007-10-07 | 2015-05-26 | Brown University | Nanostructured sorbent materials for capturing environmental mercury vapor |
| US8869992B2 (en) | 2008-02-28 | 2014-10-28 | Brown University | Lighting apparatus for capturing and stabilizing mercury |
| US8506923B2 (en) * | 2008-02-28 | 2013-08-13 | Brown University | Nanostructured sorbent materials for capturing environmental mercury vapor |
| US8491865B2 (en) | 2008-02-28 | 2013-07-23 | Brown University | Nanostructured sorbent materials for capturing environmental mercury vapor |
| US20110053766A1 (en) * | 2008-02-28 | 2011-03-03 | Brown University | Nanostructured sorbent materials for capturing environmental mercury vapor |
| CN104807999A (en) * | 2014-01-28 | 2015-07-29 | 兰州大学 | Electroselenium rapid detection test strip |
| CN104555951A (en) * | 2014-12-31 | 2015-04-29 | 烟台佳隆纳米产业有限公司 | Method for preparing and storing nano-selenium through cryogenic technology |
| CN104555951B (en) * | 2014-12-31 | 2016-08-24 | 烟台佳隆纳米产业有限公司 | A kind of method utilizing cryogenic technique preparation and preservation nanometer selenium |
| CN107167615A (en) * | 2017-05-16 | 2017-09-15 | 河南大学 | A kind of electroselenium detected for clenobuterol hydrochloride, colloidal selenium marked thing, detection card and preparation method thereof, application |
| CN108477618A (en) * | 2018-02-05 | 2018-09-04 | 江苏大学 | A kind of controllable method for preparing of pectin nanometer selenium |
| CN113383960A (en) * | 2021-06-10 | 2021-09-14 | 广州大学 | High-stability protein polypeptide-nano selenium and preparation method and application thereof |
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Addressee: Inst. of Field Blood Transfusion, Academy of Military Medical Sciences, PLA Document name: Qualified notice of first instance |
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