CN1463756A - Method for preparation of artificial bone antigen for biological origin - Google Patents
Method for preparation of artificial bone antigen for biological origin Download PDFInfo
- Publication number
- CN1463756A CN1463756A CN 02120968 CN02120968A CN1463756A CN 1463756 A CN1463756 A CN 1463756A CN 02120968 CN02120968 CN 02120968 CN 02120968 A CN02120968 A CN 02120968A CN 1463756 A CN1463756 A CN 1463756A
- Authority
- CN
- China
- Prior art keywords
- surfactant
- bone
- artificial bone
- biogenic
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Materials For Medical Uses (AREA)
- Detergent Compositions (AREA)
- Peptides Or Proteins (AREA)
Abstract
Biogenous artificial bone tissue is set inside the solution of serial surfactants for full wetting and penetration, the solution enters to the bone tissue and the fat tissue falls down to disperse in the surfactant solution and to be eliminated via water rinsing. The surfactant used may be biodegraded inside body and is non-toxic and harmless. The said process has high defatting effect and efficient antigenicity reducing effect. The processed artificial bone has even high mechanical performance, the production process has no pollution, and the product has no residual toxic matter and is convenient in storing and transportation.
Description
Affiliated technical field:
The present invention relates to bone transplanting active bio source property artificial bone technical field in the medical science Orthopeadic Surgery, relate to a kind of biogenic artificial bone that is used for specifically and go antigenic preparation method.
Background technology:
As everyone knows, it is that osteoarthrosis is because of one of most common therapeutic method such as disappearance reconstruction after the damaged and wound of treatment bone in the orthopaedics that bone is transplanted, and it is also a lot of to be used for the kind of bone materials implanted.At this wherein, property artificial bone in active bio source is because the superiority of each side such as its physicochemical property, space structure and functional adaptation is that bone is transplanted the maximum material of clinical use.And the key of transplanting success or not is to transplant the immunoreation of active bio source, back property artificial bone, causing immunoreactive is the antigen that is had in the biogenic artificial bone, how removing antigen and reduce the success that the immunoreation guarantee bone after transplanting is transplanted, is the important topic of the academia of bone transplanting in recent years.
Reducing immunoreation technology commonly used clinically has following several:
(1) deep-frozen method or freeze-drying: be that the widest allograph bone of using is at present handled and storage procedures, can effectively reduce xenogenesis, allograph bone immunogenicity, wherein more obvious with the effect of lyophilization.But protein also has been saved simultaneously inside and outside the enzyme of this method degraded bone matrix and the cell relevant with delayed hypersensitivity reaction, thereby cause the degraded of bone Induced substance in the bone matrix, damage the induced osteogenesis activity of bone, still can bring out immunoreation in various degree simultaneously.
(2) decalcification method: the decalcification bone has stronger bone induction force, can induction of vascular around undifferentiated mesenchymal cell and marrow stromal cell to chondroblast and osteoblast differentiation, but this method reduces the mechanical strength of bone.
(3) antigen extraction method: i.e. organic solvent degreasing such as chloroform methanol liquid, hydrochloric acid decalcification, H
2O
2Deproteinization, lyophilizing and sterilization treatment are as the AAA bone that Urist created.This method had not only been removed antigenicity but also had been kept the ability of induced osteogenesis, but because the toxicity of fears are entertained that chloroform/methanol, clinical practice is restricted.
(4) irradiation method, radiation technique are handled: can reduce the antigenicity of allosome (kind) bone preferably, but strong excessively radiation dose has infringement to the mechanical strength of allosome (kind).
(5) boiling method, high pressure, calcining and formaldehyde, thimerosal method are handled: though above method can reduce the immunogenicity of bone, heavy damage the biology performance of bone, or residual toxicity is bigger, or influence the induced osteogenesis activity of bone, all few at present usefulness.
For example Chinese patent CN11880115A discloses the method that a kind of defatted antigen-removing prepares bone grafting material, employing be that chemical treatment and decalcification method remove antigen, just exist above-mentioned shortcoming.
Summary of the invention:
In order to overcome the defective that exists separately in the said method, the invention provides a kind of biogenic artificial bone that is used for and go antigenic preparation method, this method had both helped remedying the shortcoming of several method, helped better reducing the immunogenicity of homogeneous allogenic bone again.The surfactant that this method adopted meets the national health food security standard, and biodegradation in vivo, and is therefore to human body safety, nontoxic; The surfactant of series has than the better effect of organic solvent degreasing, can effectively reduce the antigenicity of bone; The allosome xenogenesis bone mechanical performance of handling with this method is better than allosome (kind) bone that art methods is handled; Production process is pollution-free, the residual and storage convenient transportation of product avirulence material.
Concrete scheme of the present invention is to utilize the principle of surfactant defat, pending biogenic artificial bone osseous tissue is put into surfactant solution, through fully moistening, infiltration, make solution enter osseous tissue inside, fatty tissue comes off thereupon, surfactant is scattered in the fat and the organic substance emulsifying that split away off in the solution, sloughs through the tri-distilled water rinsing.Be characterized in comprising the following steps:
1, biogenic artificial bone bone piece is made required size
2, make the degreaser that total concentration is 20%-60% with series of surfactants, its compound method is as follows:
A, use anion surfactant: 1%-70% sodium alkyl benzene sulfonate (ABS) and 1%-80% polyoxyethylenated alcohol sodium sulfate (AES) proportioning
B, usefulness 1%-10% non-ionic surface active agent and 1%-90% yin, yang, amphoteric ionic surfactant proportioning
C, usefulness 1%-90% zwitterionic surfactant and 1%-90% yin, yang, zwitterionic surfactant proportioning
3, pending bone piece is soaked in the degreaser that is disposed
4, be 20-60 ℃ in temperature, power is that sonic oscillation washs or stirred 24-240 hour under the 60%-100% condition
5, with tri-distilled water vibration washing 10-20 time
6, lyophilizing 2-200 hour
7, vacuum packaging, nucleic radiation or oxirane disinfection sterilization
Above-mentioned kinds of surfactants can be: anion surfactant, non-ionic surface active agent, cationic surfactant, zwitterionic surfactant.
The specific embodiment:
Embodiment 1:
1. biogenic artificial bone bone piece is made required size
2, use anion surfactant: it is 50% degreaser that 1%-70% sodium alkyl benzene sulfonate (ABS) and 1%-80% polyoxyethylenated alcohol sodium sulfate (AES) proportioning are made total concentration
3, pending bone piece is soaked in the degreaser that is disposed
4, be 20-60 ℃ in temperature, power is that sonic oscillation washs or stirred 24-240 hour under the 60%-100% condition
5, with tri-distilled water vibration washing 10-20 time
6, lyophilizing 2-200 hour
7, vacuum packaging, nucleic radiation or oxirane disinfection sterilization
Embodiment 2:
The present embodiment difference from Example 1 is the configuration of degreaser, and making total concentration with 1%-10% non-ionic surface active agent and 1%-90% yin, yang, amphoteric ionic surfactant proportioning is 60% degreaser.Other steps are the same.
Embodiment 3:
With last two embodiment differences also be the configuration that is degreaser, making total concentration with 1%-90% zwitterionic surfactant and 1%-90% yin, yang, zwitterionic surfactant proportioning is 40% degreaser.Other steps are the same.
Claims (2)
1. one kind is used for the biogenic artificial bone and goes antigenic preparation method, pending biogenic artificial bone osseous tissue is put into surfactant solution, through fully moistening, infiltration, make solution enter osseous tissue inside, fatty tissue comes off thereupon, surfactant is scattered in the fat and the organic substance emulsifying that split away off in the solution, sloughs through the tri-distilled water rinsing.Be characterized in comprising the following steps:
(1) biogenic artificial bone bone piece is made required size
(2) make the degreaser that total concentration is 20%-60% with series of surfactants, its compound method is as follows:
D, use anion surfactant: 1%-70% sodium alkyl benzene sulfonate (ABS) and 1%-80% polyoxyethylenated alcohol sodium sulfate (AES) proportioning
E, usefulness 1%-10% non-ionic surface active agent and 1%-90% yin, yang, amphoteric ionic surfactant proportioning
F, usefulness 1%-90% zwitterionic surfactant and 1%-90% yin, yang, zwitterionic surfactant proportioning
(3) pending bone piece is soaked in the degreaser that is disposed
(4) be 20-60 ℃ in temperature, power is that sonic oscillation washs or stirred 24-240 hour under the 60%-100% condition
(5) with tri-distilled water vibration washing 10-20 time
(6) lyophilizing 2-200 hour
(7) vacuum packaging, nucleic radiation or oxirane disinfection sterilization
2, preparation method according to claim 1, the above kinds of surfactants can be: anion surfactant, non-ionic surface active agent, cationic surfactant, zwitterionic surfactant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02120968 CN1216651C (en) | 2002-06-06 | 2002-06-06 | Method for preparation of artificial bone antigen for biological origin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02120968 CN1216651C (en) | 2002-06-06 | 2002-06-06 | Method for preparation of artificial bone antigen for biological origin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1463756A true CN1463756A (en) | 2003-12-31 |
| CN1216651C CN1216651C (en) | 2005-08-31 |
Family
ID=29742683
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02120968 Expired - Fee Related CN1216651C (en) | 2002-06-06 | 2002-06-06 | Method for preparation of artificial bone antigen for biological origin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1216651C (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100496623C (en) * | 2006-06-14 | 2009-06-10 | 中国人民解放军第三军医大学第一附属医院 | Heterogenic deproteinized osseous stent material and its preparation method |
| CN1839773B (en) * | 2005-04-01 | 2011-05-04 | 祝天经 | Method for making heterogeneous bone free of antibody |
| CN104174067A (en) * | 2013-05-22 | 2014-12-03 | 烟台正海生物技术有限公司 | Natural inorganic bone matrix and preparation method |
| CN107096070A (en) * | 2017-03-09 | 2017-08-29 | 中国医学科学院阜外医院 | A kind of de- cell pulmonary branches frame and preparation method thereof |
| CN111069149A (en) * | 2019-12-20 | 2020-04-28 | 河北鑫康辰生物技术有限公司 | Medical method for cleaning allogeneic bone |
| CN115645617A (en) * | 2022-09-09 | 2023-01-31 | 北京鑫康辰医学科技发展有限公司 | Preparation method of bone matrix gel with biological activity |
-
2002
- 2002-06-06 CN CN 02120968 patent/CN1216651C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1839773B (en) * | 2005-04-01 | 2011-05-04 | 祝天经 | Method for making heterogeneous bone free of antibody |
| CN100496623C (en) * | 2006-06-14 | 2009-06-10 | 中国人民解放军第三军医大学第一附属医院 | Heterogenic deproteinized osseous stent material and its preparation method |
| CN104174067A (en) * | 2013-05-22 | 2014-12-03 | 烟台正海生物技术有限公司 | Natural inorganic bone matrix and preparation method |
| CN107096070A (en) * | 2017-03-09 | 2017-08-29 | 中国医学科学院阜外医院 | A kind of de- cell pulmonary branches frame and preparation method thereof |
| CN107096070B (en) * | 2017-03-09 | 2020-08-21 | 中国医学科学院阜外医院 | Decellularized lung scaffold and preparation method thereof |
| CN111069149A (en) * | 2019-12-20 | 2020-04-28 | 河北鑫康辰生物技术有限公司 | Medical method for cleaning allogeneic bone |
| CN115645617A (en) * | 2022-09-09 | 2023-01-31 | 北京鑫康辰医学科技发展有限公司 | Preparation method of bone matrix gel with biological activity |
| CN115645617B (en) * | 2022-09-09 | 2023-07-25 | 北京鑫康辰医学科技发展有限公司 | Preparation method of bone matrix gel with bioactivity |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1216651C (en) | 2005-08-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2167148B1 (en) | Method of treating tissue | |
| TWI600660B (en) | Preparation of high purity collagen particles and used thereof | |
| AU2002309898B2 (en) | EB matrix production from fetal tissues and its use for tissue repair | |
| CN1072970C (en) | Process for treating bone tissue and corresponding implantable biomaterials | |
| JP2002529201A (en) | How to pool organizations | |
| RU2665962C1 (en) | Bioresorable biological matrix for substitution of bone tissue defects and method of its obtaining | |
| CN108478868B (en) | Preparation method and application of injectable allogeneic adipose acellular matrix particles | |
| EP1929862A1 (en) | Allograft tissue purification process for cleaning bone | |
| AU2002309898A1 (en) | EB matrix production from fetal tissues and its use for tissue repair | |
| CN1214821C (en) | Preparing method for heteroossein base materials | |
| CA2263557C (en) | Implant consisting of a carrier material containing medical substances and method to produce such an implant | |
| CN101380488B (en) | Preparation method of bovine cortical bone grafting material | |
| CN1216651C (en) | Method for preparation of artificial bone antigen for biological origin | |
| EP2236544A1 (en) | Collagen Implant | |
| RU2691983C1 (en) | Method for purification, modification and sterilization of bone tissue and skin matrix derivatives using supercritical fluid | |
| CN112957533A (en) | Preparation method of imitation cortical bone repair material | |
| WO2012121448A1 (en) | Bone graft substitute production method | |
| CN1200740C (en) | Method for preparing artificial bone | |
| RU2619870C1 (en) | Method for biologically active implants obtaining | |
| KR20030067676A (en) | Volume maintaining osteoinductive/osteoconductive compositions | |
| EP0683681B1 (en) | Injectable implant for correcting wrinkles and skin hollows | |
| CN105169493B (en) | A kind of preparation method of artificial xenogeneic skin | |
| EP3927388A1 (en) | Decellularized muscle matrices and methods for making and using same | |
| EP1414368A2 (en) | Eb matrix production from fetal tissues and its use for tissue repair |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20050831 Termination date: 20190606 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |