CN1455256A

CN1455256A - Method of discriminating medicinal ingredient of sculellaria barbata

Info

Publication number: CN1455256A
Application number: CN 03117343
Authority: CN
Inventors: 吴建滨
Original assignee: Dechangxiang Medical Industry Co Ltd Guiyang
Current assignee: Dechangxiang Medical Industry Co Ltd Guiyang
Priority date: 2003-02-19
Filing date: 2003-02-19
Publication date: 2003-11-12
Anticipated expiration: 2023-02-19
Also published as: CN1205477C

Abstract

The 1g powder of the medicinal material to be discriminated is prepared as the solution to be tested. The comparison solution of the medicinal material is prepared by fetching standard barbat skullcap medicinal material. Then, with methanol 1ml being added to scutellarin, the solution with 1mg scutellarin in 1ml methanol is prepared as the solution of the reference substance. By using thin-layer chromatography, 5% ethanol solution of ferric chloride is sprayed on the spread three solutions. When the chromatogram of the sample is positioned at the relevant position of the chromatogram of the medicinal material, if both fluorescent speckles are in same color as well as if the chromatogram of the sample and the chromatogram of the reference substance have speckles with same sap green at the relevant position, then, the medicinal material to be discriminated contains scutellarin component. The invention possesses the features of high accuracy, good specialization and repeatability.

Description

Differentiate the method for Sculellaria barbata medicinal ingredient

Technical field:

The present invention relates to a kind of method of differentiating the Sculellaria barbata medicinal ingredient, belong to and differentiate the medicinal ingredient technical field.

Background technology:

At present, the general method of differentiating the Sculellaria barbata medicinal ingredient in the prior art, mainly be that experience according to the people judges, therefore exist that poor accuracy, error are big, no specificity, shortcoming that reappearance is low, and existing discrimination method also requires the discriminating personnel that the Sculellaria barbata medicinal material is had special identification of experience, this is for general reviewer, be difficult to accurately differentiate and judge by the mirror medicine whether contain the Sculellaria barbata medicinal ingredient, therefore the discrimination method that existing experience by the people is differentiated the Sculellaria barbata medicinal ingredient in use, and is still very undesirable.

Summary of the invention:

The objective of the invention is: provide that a kind of specificity is strong, favorable reproducibility, simple to operate, and can accurately identify the method for the discriminating Sculellaria barbata medicinal ingredient that whether contains the Sculellaria barbata medicinal material, to overcome the deficiencies in the prior art.

The present invention is achieved in that it comprises the employing thin-layered chromatography, is characterized in that: get and differentiated medicinal powder 1g, porphyrize, 20ml adds diethyl ether, refluxed 30 minutes, and filtered, abandon ether solution, ether is removed in dregs of a decoction volatilization, add methyl alcohol 30ml, reflux 30 minutes is filtered, the filtrate evaporate to dryness, residue adds water 5ml makes its dissolving, and it is put the D-101 that handled well only on the product type macroporous adsorptive resins, takes off earlier with 40ml water, discard eluent, use 20% methyl alcohol 30ml wash-out again, collect eluent, evaporate to dryness, residue adds methyl alcohol 1ml dissolving, as need testing solution; Other gets standard Sculellaria barbata control medicinal material 1g, makes control medicinal material solution as stated above; Get the scutellarin reference substance again, add methyl alcohol and make the solution that every 1ml contains the 1mg scutellarin, in contrast product solution; Test according to thin-layered chromatography, draw each 5～10ul of above-mentioned three kinds of solution, putting in the same carboxymethylcellulose sodium solution that contains 4% sodium acetate respectively is on the silica gel g thin-layer plate of binder, with ethyl acetate-butanone-formic acid-water, its weight portion ratio is 5: 3: 1: 1, be developping agent, pre-equilibration 20 minutes launches, take out, dry, the spray with 5% ferric trichloride ethanolic solution, when in the test sample chromatogram with the corresponding position of control medicinal material chromatogram on, the spot that shows same color, and with the corresponding position of reference substance chromatogram on, when showing identical dirty-green spot, promptly differentiate to containing the Sculellaria barbata medicinal ingredient.

Owing to adopted technique scheme, the present invention compares with the method for existing discriminating Sculellaria barbata medicinal ingredient, the present invention not only has accuracy height, advantage that driscrimination error is little, but also have that specificity is strong, favorable reproducibility, advantage simple to operate, use the present invention not need veteran reviewer can identify tested medicinal material accurately, easily and whether contain the Sculellaria barbata medicinal ingredient.

Embodiment:

Embodiments of the invention: it comprises the employing thin-layered chromatography, it is characterized in that: get and differentiated medicinal powder 1g, porphyrize, 20ml adds diethyl ether, refluxed 30 minutes, and filtered, abandon ether solution, ether is removed in dregs of a decoction volatilization, add methyl alcohol 30ml, reflux 30 minutes is filtered, the filtrate evaporate to dryness, residue adds water 5ml makes its dissolving, and it is put the D-101 that handled well only on the product type macroporous adsorptive resins, takes off earlier with 40ml water, discard eluent, use 20% methyl alcohol 30ml wash-out again, collect eluent, evaporate to dryness, residue adds methyl alcohol 1ml dissolving, as need testing solution; Other gets standard Sculellaria barbata control medicinal material 1g, porphyrize, and the 20ml that adds diethyl ether refluxed 30 minutes, filter, abandon ether solution, ether is removed in dregs of a decoction volatilization, adds methyl alcohol 30ml, reflux 30 minutes is filtered the filtrate evaporate to dryness, residue adds water 5ml makes its dissolving, and it is put the D-101 that handled well only on the product type macroporous adsorptive resins, takes off earlier with 40ml water, discard eluent, use 20% methyl alcohol 30ml wash-out again, collect eluent, evaporate to dryness, residue add methyl alcohol 1ml dissolving, promptly make control medicinal material solution; Get the scutellarin reference substance again, add methyl alcohol and make the solution that every 1ml contains the 1mg scutellarin, in contrast product solution; According to " the method for the thin-layered chromatography test that an appendix VI of Chinese pharmacopoeia version in 2000 B is introduced, draw each 5～10ul of above-mentioned three kinds of solution, putting in the same carboxymethylcellulose sodium solution that contains 4% sodium acetate respectively is on the silica gel g thin-layer plate of binder, with ethyl acetate-butanone-formic acid-water, its weight portion ratio is 5: 3: 1: 1, be developping agent, pre-equilibration 20 minutes, launch, take out, dry, the spray with 5% ferric trichloride ethanolic solution, when in the test sample chromatogram with the corresponding position of control medicinal material chromatogram on, the spot that shows same color, and with the corresponding position of reference substance chromatogram on, when showing identical dirty-green spot, promptly differentiate to containing the Sculellaria barbata medicinal ingredient.

Claims

1, a kind of method of differentiating the Sculellaria barbata medicinal ingredient, it comprises the employing thin-layered chromatography, it is characterized in that: get and differentiated medicinal powder 1g, porphyrize, the 20ml that adds diethyl ether refluxed 30 minutes, filter, abandon ether solution, ether is removed in dregs of a decoction volatilization, adds methyl alcohol 30ml, reflux 30 minutes, filter, filtrate evaporate to dryness, residue add water 5ml makes its dissolving, it is put the D-101 that handled well only on the product type macroporous adsorptive resins, take off earlier with 40ml water, discard eluent, use 20% methyl alcohol 30ml wash-out again, collect eluent, evaporate to dryness, residue add methyl alcohol 1ml dissolving, as need testing solution; Other gets standard Sculellaria barbata control medicinal material 1g, makes control medicinal material solution as stated above; Get the scutellarin reference substance again, add methyl alcohol and make the solution that every 1ml contains the 1mg scutellarin, in contrast product solution; Test according to thin-layered chromatography, draw each 5～10ul of above-mentioned three kinds of solution, putting in the same carboxymethylcellulose sodium solution that contains 4% sodium acetate respectively is on the silica gel g thin-layer plate of binder, with ethyl acetate-butanone-formic acid-water, its weight portion ratio is 5: 3: 1: 1, be developping agent, pre-equilibration 20 minutes launches, take out, dry, the spray with 5% ferric trichloride ethanolic solution, when in the test sample chromatogram with the corresponding position of control medicinal material chromatogram on, the spot that shows same color, and with the corresponding position of reference substance chromatogram on, when showing identical dirty-green spot, promptly differentiate to containing the Sculellaria barbata medicinal ingredient.