CN1436842A - Purple glossy ganoderma with high polysaccharide content and its fermenting production process - Google Patents
Purple glossy ganoderma with high polysaccharide content and its fermenting production process Download PDFInfo
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Abstract
The present invention belongs to the field of microbe technology, and is especially one strain of purple glossy ganoderma with high polysaccharide content in the preservation number of CGMCC No.0742. The purple glossy ganoderma culture in potato culture medium or soybean culture medium has mycelial filament powder with polysaccharide content as high as 12-14 wt%. Now, the technology of the present invention has been used in industrial production.
Description
Technical field the invention belongs to microbial technology field.Specifically, relate to the purple sesame that a plant height produces polysaccharide, adopt the method for semi-solid ferment, can make to contain purple sesame polysaccharide in the per 100 gram hypha powders of purple sesame polysaccharide of its generation and restrain up to 12-14.At present, the technology of the present invention has entered suitability for industrialized production and has used, and product is by the approval of the The People's Republic of China Ministry of Health, and card number is: No. the 0469th, hygienic food and health number (2002), now to produce in batches, and product puts goods on the market.
The background technology glossy ganoderma is a kind of medicinal, high large-scale basidiomycete of the value that keeps healthy.Present known Ganoderma has kind more than 100 approximately, and is wherein extensive with red sesame and purple sesame purposes.Both at home and abroad the research of red sesame bacterial classification, red sesame Products Development, pharmacological research are all done a lot of work in recent years, the result shows: the glossy ganoderma clinical application in neurasthenic efficient be 96%, it has analgesia, calmness, stable, hypnosis, antibechic, relievings asthma, phlegm-dispelling functions; Glossy ganoderma can strengthen scheming convergent force, coronary artery dilator; That ganoderan, peptidoglycan have is hypoglycemic, endocrine regulation and metabolic effect; The protecting liver and detoxication effect, how 95.18%[comes Ying Dengzhu with glossy ganoderma treatment chronic viral hepatitis total effective rate " research and the progress-11 (summary) of glossy ganoderma ", institute is checked in Ministry of Health's Food Hygiene Surveillance].Purple sesame is the elaboration of the traditional glossy ganoderma of China.Therefore purple sesame seldom can see the product of purple sesame because more difficult worker tames cultivation on market.April calendar year 2001, the development of medicinal fungi that file encourages to comprise three kinds of lucidum varieties of purple sesame was assigned by the Ministry of Health.
At present, domestic and international research to ganoderan generally all rests in fruitbody polysaccharide extraction and the extraction of liquid mycelium polysaccharides.Along with the rise of modern biotechnology, cultivate Ganoderma mycelium by biofermentation technique and formed industrialized scale.China's achievement in research in this respect is mainly reflected in: the mycelial culture condition of liquid towards fermentation culture is studied, and the mycelium meta-bolites is studied in the liquid towards culturing process, but great majority are research object with red sesame polysaccharide, to the research of purple sesame but seldom.It is domestic that research yet there are no report to purple sesame solid state fermentation working condition.Be that the research of mycelium meta-bolites and secondary metabolite is more rare in the research of research object, the solid-state culturing process of purple sesame particularly with purple sesame.
The present invention adopts the edible soybean to be substratum, to carry out mycelium polysaccharides and purple sesame solid state fermentation mycelium meta-bolites and the secondary metabolite that solid state fermentation produces with purple sesame bacterial classification YTZ9K1, and present technique is that the purple sesame protective foods that integrates protective foods was not also met report at home.
Summary of the invention the purpose of this invention is to provide the purple sesame bacterial strain that a plant height produces polysaccharide, utilizes the semi-solid ferment method, and the mycelium polysaccharides that this bacterium produces is well beyond the level of general report.
The present invention adopts Co
60The method of induced mutations, seed selection filter out the purple sesame bacterial classification of strain YTZ9kl, are accredited as purple sesame (Ganoderma sinense J.D.Zhao, L.W.Hsu ﹠amp through Institute of Microorganism, Academia Sinica; X.Q.Zhang). this bacterial classification has been deposited in Chinese microbial preservation council common micro-organisms preservation administrative center on April 30th, 2002, the purple sesame (Ganoderma sinense YTZ9K1) of preservation bacterial classification, preserving number: CGMCC NO.0742, carry out semi-solid ferment with this bacterium, per 100 gram hypha powders can obtain purple sesame polysaccharide up to 14 grams about, than former bacterial classification and reported that the polysaccharide yield of glossy ganoderma had improved 50-100%.
Another object of the present invention provides the optimal conditions of fermentation of purple sesame bacterial classification CGMCC NO.0742.Female kind and first order seed adopt the potato culture that contains potato 20-35%, glucose 5-10%, peptone 2-5%; Secondary seed and fermentative production are to adopt the soya broth that contains soya bean 10-20%, Semen Maydis powder 5-10%, in addition, also add the inorganic salts auxiliary material in substratum, as 1 sal epsom, 2 potassium primary phosphates and 3 lime carbonate.Substratum through 120 ℃, 1.4Kg/cm2,30 minutes the sterilization.Inoculum size is 5-10%, 28 ± 2 ℃ of female kind, first order seed and secondary seed culture temperature, relative humidity 80 ± 5%.
Female kind and first order seed incubation time are 5-7 days, and the secondary seed incubation time is 7-10 days, and the incubation time of fermentative production is 12-15 days, 26 ± 2 ℃ of culture temperature.
CGMCC NO.
0742The mycelium that purple sesame bacterial strain is produced, be that industrialization liquid submerged fermentation technical development from common application becomes semi-solid state shallow-layer fermentation technique, research according to growth conditions, fermentation condition and the fermentation kinetics of the semi-solid ferment of purple sesame, design the semi-automatic semi-solid state hypha fermentation production line of 10 tonnes of more complete annual outputs, seen the technical process accompanying drawing for details.
The separation of ganoderan and the step of purifying are: Ganoderma powder is through flooding, remove protein with lead plumbate, 80% dehydrated alcohol precipitation polysaccharide, acetone then, alcohol replaces washing polysaccharide, use distilled water heating for dissolving polysaccharide again, add dehydrated alcohol, make to contain alcohol amount and reach 20%, centrifugal removal impurity, obtain polysaccharide liquid, continue to add dehydrated alcohol, make to contain alcohol amount and reach 80%, standing over night, centrifugal 20 minutes of 4000r/m, get ganoderma lucidum crude polysaccharide, behind the water dissolution polysaccharide, flowing water dialysis liquid glucose 3 days, add dehydrated alcohol then successively, make and contain alcohol amount and reach 50%, 60%, 70%, 80% standing over night, natural air drying gets four kinds of ganoderan I, II, III, IV.
Embodiment is further specified by following examples in order to understand the present invention better.Embodiment 1:CGMCC NO.
0742The mycelium substratum that purple sesame bacterial strain is produced:
1) stock mare tinkling of pieces of jade potato substratum: potato 20%～35%; Glucose 5%～10%; Peptone 2%～5% etc.120 ℃, 1.4 kg/cm, autoclaving 30 minutes.
2) first order seed substratum: with 1).
3) secondary seed soya broth: soya bean 10%～20%; Semen Maydis powder 5%～10%; 1 auxiliary material 0.5%～1%; 2 auxiliary materials 0.5%～1%; 3 auxiliary materials 0.5%～1%.120 ℃, 1.4 kg/cm, autoclaving 30 minutes.
4) produce with kind of a substratum: soya bean 10%～20%; Semen Maydis powder 5%～10%; 1 auxiliary material 0.5%～1%; 2 auxiliary materials 0.5%～1%; 0.5%～1%, 120 ℃ of 3 auxiliary materials, 1.4 kg/cm, autoclaving 90 minutes.Inoculation:
The female kind: super quiet worktable aseptic technique, inoculum size 1～2% is put and is left standstill cultivation in the incubator.
The one-level kind: super quiet worktable aseptic technique, inoculum size 5～10% is put shaking table cultivation in the incubator.
The secondary kind: super quiet worktable aseptic technique, inoculum size 5～10% is put aseptic shaking table and is cultivated.
Produce inoculation: aseptic aseptic technique, inoculum size 5～10% is put aseptic and is left standstill cultivation.Culture temperature, time and humidity:
Female level is planted: 28 ℃ ± 2 ℃, and incubation time, 5～7 days, humidity 85～90%
The one-level kind: 28 ℃ ± 2 ℃, incubation time 5～7 days, humidity 85～90%.
The secondary kind: 27 ℃ ± 2 ℃, incubation time 7～10 days, humidity 80～85%.
Fermentative production: 26 ℃ ± 2 ℃, incubation time 12～15 days, humidity 75～85%.The separation and the purifying of embodiment 2 purple sesame polysaccharide
The separation of purple sesame polysaccharide and the step of purifying are: the purple sesame hypha powder by fermentative preparation is after flooding, remove protein with lead plumbate, 80% dehydrated alcohol precipitation polysaccharide, acetone then, alcohol replaces washing polysaccharide, use distilled water heating for dissolving polysaccharide again, add dehydrated alcohol, make to contain alcohol amount and reach 20%, centrifugal removal impurity, obtain polysaccharide liquid, continue to add dehydrated alcohol, make to contain alcohol amount and reach 80%, after the standing over night, through 4000rpm centrifugal 20 minutes, get ganoderma lucidum crude polysaccharide, behind the water dissolution polysaccharide, flowing water dialysis liquid glucose 3 days, add dehydrated alcohol then successively, make and contain alcohol amount and reach 50%, 60%, 70%, 80% standing over night, natural air drying gets four kinds of purple sesame polysaccharide I, II, III, IV.
Claims (7)
1. a purple sesame bacterium that produces ganoderan is CGMCC NO.0742.
2. purple sesame bacterium CGMCC NO.0742 according to claim 1, female kind and one-level neutron adopt the potato culture that contains potato 20-35%, glucose 5-10%, peptone 2-5%; Secondary seed and fermentative production are to adopt the soya broth that contains soya bean 10-20%, Semen Maydis powder 5-10%, also will add auxiliary material sal epsom, potassium primary phosphate and the lime carbonate of 0.5-1.0% and cultivate.
3. the cultivation of purple sesame bacterium CGMCC NO.0742 according to claim 2, inoculum size is 5-10%, 28 ± 2 ℃ of female kind, first order seed and secondary seed culture temperature, female kind and first order seed incubation time are 5-7 days, the secondary seed incubation time is 7-10 days, the incubation time of fermentative production is 12-15 days, 26 ± 3 ℃ of culture temperature, relative humidity 80 ± 5%.
4. purple sesame bacterium CGMCC NO.0742 fermentative production according to claim 1, the purple sesame polysaccharide of average per 100 gram hypha powders is not less than 14 grams.
5. purple sesame hypha powder polysaccharide according to claim 4, the separation of this purple sesame polysaccharide and the purifying step is: through the purple sesame hypha powder of fermentative preparation after flooding, remove protein with lead plumbate, 80% dehydrated alcohol precipitation polysaccharide, acetone then, alcohol replaces washing polysaccharide, uses distilled water heating for dissolving polysaccharide again, adds dehydrated alcohol, make and contain alcohol amount and reach 20%, centrifugal removal impurity obtains polysaccharide liquid, continues to add dehydrated alcohol, make and contain alcohol amount and reach 80%, after the standing over night, through 4000rpm centrifugal 20 minutes, purple sesame Crude polysaccharides, behind the water dissolution polysaccharide, flowing water dialysis liquid glucose 3 days adds dehydrated alcohol then successively, makes to contain the alcohol amount and reach 50%, 60%, 70%, 80% standing over night, natural air drying gets four kinds of ganoderan I, II, III, IV.
6. the application of purple sesame bacterium CGMCC NO.0742 according to claim 1 in producing purple sesame polysaccharide.
7. according to claim 2,3 and/or 4 described methods, the application in producing ganoderan.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1317391C (en) * | 2004-07-01 | 2007-05-23 | 阎波 | Method for extracting ganoderan by solid fermentation |
| CN100372923C (en) * | 2006-01-20 | 2008-03-05 | 中山大学 | Method for promoting Chinese Ganoderma spore gemination |
| CN101831472A (en) * | 2010-06-09 | 2010-09-15 | 福建省农业科学院农业工程技术研究所 | Method for preparing edible fungi soluble polysaccharide through solid fermentation by taking bean dregs as raw materials |
| CN101376873B (en) * | 2007-08-29 | 2011-03-30 | 上海医药工业研究院 | Chinese Ganoderma fermentation method and Chinese Ganoderma mycelium prepared thereby |
| CN102771769A (en) * | 2012-07-20 | 2012-11-14 | 黄晓青 | Ganoderma sinensis health product and preparation method thereof |
| CN102827298A (en) * | 2011-06-13 | 2012-12-19 | 上海医药工业研究院 | Uniform polysaccharide of ganoderma sinensis liquid deep layer fermentation mycelium, and preparation method thereof |
| CN110558155A (en) * | 2016-10-14 | 2019-12-13 | 北京京诚生物科技有限公司 | Ganoderma sinensis and high-yield and high-efficiency cultivation method thereof |
| CN112662566A (en) * | 2020-12-15 | 2021-04-16 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Ganoderma lucidum spore-less variety with high yield of polysaccharide and artificial cultivation method thereof |
| CN113999777A (en) * | 2021-12-13 | 2022-02-01 | 东莞市英芝堂生物工程有限公司 | Ganoderma sinensis with high polysaccharide yield and fermentation production process thereof |
-
2002
- 2002-10-26 CN CN 02134967 patent/CN1202238C/en not_active Expired - Lifetime
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1317391C (en) * | 2004-07-01 | 2007-05-23 | 阎波 | Method for extracting ganoderan by solid fermentation |
| CN100372923C (en) * | 2006-01-20 | 2008-03-05 | 中山大学 | Method for promoting Chinese Ganoderma spore gemination |
| CN101376873B (en) * | 2007-08-29 | 2011-03-30 | 上海医药工业研究院 | Chinese Ganoderma fermentation method and Chinese Ganoderma mycelium prepared thereby |
| CN101831472A (en) * | 2010-06-09 | 2010-09-15 | 福建省农业科学院农业工程技术研究所 | Method for preparing edible fungi soluble polysaccharide through solid fermentation by taking bean dregs as raw materials |
| CN101831472B (en) * | 2010-06-09 | 2012-05-23 | 福建省农业科学院农业工程技术研究所 | Method for preparing edible fungi soluble polysaccharide through solid fermentation by taking bean dregs as raw materials |
| CN102827298A (en) * | 2011-06-13 | 2012-12-19 | 上海医药工业研究院 | Uniform polysaccharide of ganoderma sinensis liquid deep layer fermentation mycelium, and preparation method thereof |
| CN102827298B (en) * | 2011-06-13 | 2015-12-02 | 上海医药工业研究院 | Purple sesame liquid submerged fermentation mycelium homogeneous polysaccharide and preparation method thereof |
| CN102771769A (en) * | 2012-07-20 | 2012-11-14 | 黄晓青 | Ganoderma sinensis health product and preparation method thereof |
| CN102771769B (en) * | 2012-07-20 | 2015-05-27 | 北京厚文知识产权顾问有限公司 | Ganoderma sinensis health product and preparation method thereof |
| CN110558155A (en) * | 2016-10-14 | 2019-12-13 | 北京京诚生物科技有限公司 | Ganoderma sinensis and high-yield and high-efficiency cultivation method thereof |
| CN112662566A (en) * | 2020-12-15 | 2021-04-16 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Ganoderma lucidum spore-less variety with high yield of polysaccharide and artificial cultivation method thereof |
| CN113999777A (en) * | 2021-12-13 | 2022-02-01 | 东莞市英芝堂生物工程有限公司 | Ganoderma sinensis with high polysaccharide yield and fermentation production process thereof |
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