CN1433772A - Use of angelica polysaccharide in preparation of analgesic - Google Patents
Use of angelica polysaccharide in preparation of analgesic Download PDFInfo
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- CN1433772A CN1433772A CN 03118538 CN03118538A CN1433772A CN 1433772 A CN1433772 A CN 1433772A CN 03118538 CN03118538 CN 03118538 CN 03118538 A CN03118538 A CN 03118538A CN 1433772 A CN1433772 A CN 1433772A
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- radix angelicae
- angelicae sinensis
- analgesic
- sinensis polysaccharide
- pain
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Abstract
The present invention relates to an application of angelica polysaccharide in preparation of analgesic with obvious action of stopping pain and obvious therapeutic effect for curing "dysmenorrhea". The can be made into capsule, tablet, granules preparation and injection preparation.
Description
Technical field
The present invention relates to the application of Radix Angelicae Sinensis polysaccharide as the preparation analgesic.
Background technology
The angelica tonic is the dry root of Umbelliferae (umbuiferae) angelica herbaceos perennial Radix Angelicae Sinensis [Angelica Sinensis (Oliv) .Diels].Radix Angelicae Sinensis has benefiting blood and regulating blood circulation, blood circulation promoting and blood stasis dispelling, and the effect of menstruction regulating and pain relieving is often used Radix Angelicae Sinensis and other medicines compatibility according to disease clinically, is used for diseases such as menoxenia, dysmenorrhea, headache due to deficiency of blood, waist abdomen pain.
The chemical composition of Radix Angelicae Sinensis comprises volatile oil and water-soluble component two large divisions.Early stage research was once pointed out Radix Angelicae Sinensis oil to various animal isolated uterines, no matter the person of becoming pregnant or unpregnancy person have the effect of relaxing.It has made explanation for Radix Angelicae Sinensis is used for dysmenorrhea treatment to a certain extent.
Radix Angelicae Sinensis polysaccharide is the Radix Angelicae Sinensis water soluble part, is further to prepare gained from the angelol hypostasis.Radix Angelicae Sinensis polysaccharide has been proved to be and has had complicated biological activity in recent years, can regulate body's immunological function, the caused immunologic function of antagonism 17-hydroxy-11-dehydrocorticosterone suppresses, and prevents the minimizing of peripheral leukocytes and the irradiation after effect in the radiation damage, enhancing body hemopoietic function and antitumor action.But whether the Radix Angelicae Sinensis water-soluble portion has analgesic activity do not appear in the newspapers, the effective ingredient of Radix Angelicae Sinensis performance analgesic activity is still unclear at present in addition.
Pain is common clinical symptoms, and seek non-safely and effectively addicted analgesics thing is the problem that the medicine worker attaches great importance to always." dysmenorrhea " be common gynecological disease especially, and the traditional Chinese medical science thinks that its pathogeny mainly is a QI and blood being obstructed, due to the hematogenous blockage.How Radix Angelicae Sinensis searches out its effective therapeutic component to carry out modernization of Chinese medicine reform as gynecological's key medicine of the traditional Chinese medical science, makes it meet the modern formulation requirement, is the research focus of medicine educational circles.
Summary of the invention
Problem to be solved by this invention provides the application of a kind of Radix Angelicae Sinensis polysaccharide as the preparation analgesic.
Technical scheme provided by the invention is that Radix Angelicae Sinensis polysaccharide is used to prepare analgesic.
The present invention is used to prepare analgesic with Radix Angelicae Sinensis polysaccharide, is about to Radix Angelicae Sinensis polysaccharide and is used to prepare capsule, tablet, granule and injection as effective ingredient, and the gained medicine has significant analgesia role, and especially treatment " dysmenorrhea " effect is remarkable.
The specific embodiment
Embodiment 1: prepare the analgesic medicine with Radix Angelicae Sinensis polysaccharide: Radix Angelicae Sinensis polysaccharide adds excipient, makes tablet according to a conventional method.
Embodiment 2: prepare the analgesic medicine with Radix Angelicae Sinensis polysaccharide: Radix Angelicae Sinensis polysaccharide adds excipient, makes capsule according to a conventional method.
Embodiment 3: prepare the analgesic medicine with Radix Angelicae Sinensis polysaccharide: Radix Angelicae Sinensis polysaccharide is dissolved in the water for injection, makes the Radix Angelicae Sinensis polysaccharide injection of 2ml according to a conventional method, wherein contain Radix Angelicae Sinensis polysaccharide 0.25g.1. Radix Angelicae Sinensis polysaccharide the results are shown in Table 1 to the influence experiment of female mice writhing response due to pain model-acetic acid.
The influence of writhing response due to the table 1 Radix Angelicae Sinensis polysaccharide Dichlorodiphenyl Acetate.N (routine number)=10, x ± s (mean ± standard deviation)
Writhing response
Grouping dosage (gkg
-1)
Incubation period, (min) number of times was (inferior/30min) protective rate (%)
Model group/5.2 ± 1.5 31.2 ± 8.0/
Radix Angelicae Sinensis polysaccharide 0.075 7.2 ± 1.3
1)19.4 ± 6.6
1)37.8
0.250 20.1 ± 7.8
2)4.8 ± 4.1
2)84.6 annotate: compare with model control group,
1)P＜0.05,
2)P＜0.001.
2. Radix Angelicae Sinensis polysaccharide is tested the influence that hot plate method causes the pain effect, and this experiment selects for use classical medicine aspirin as positive control drug.The results are shown in Table 2.
Table 2 Radix Angelicae Sinensis polysaccharide causes the influence of pain to the female mice hot plate method.n＝10， x±s
Time threshold of pain raising rate is improved in the threshold of pain time threshold of pain behind the time administration of the preceding threshold of pain of dosed administration
Grouping
(g·kg
-1) (s) (s) (s) (％)
Model group/20.4 ± 5.8 19.8 ± 7.8 2.5 ± 3.2 12.2
Radix Angelicae Sinensis polysaccharide 0.075 21.4 ± 7.4 24.6 ± 9.7 6.3 ± 7.3 29.4
0.250 24.4±3.5 36.3±10.9 11.9±8.7
1) 48.7
Aspirin 0.250 24.8 ± 3.4 38.0 ± 12.0 12.3 ± 9.2
1)49.6 annotate: compare with matched group,
1)P＜0.05.
3. Radix Angelicae Sinensis polysaccharide is to " dysmenorrhea model "---and the influence experiment of Mouse Uterus spasm is brought out in diethylstilbestrol and oxytocin, and this experiment selects for use the sure YUEYUESHU CHONGJI of present curative effect as positive control drug.The results are shown in Table 3.
Table 3 Radix Angelicae Sinensis polysaccharide is to the influence of diethylstilbestrol and oxytocin induced mice hysterospasm.n＝10， x±s
Writhing response
Grouping dosage (gkg
-1)
Incubation period, (min) number of times was (inferior/30min) incidence rate (%)
Model group/14.1 ± 5.1 6.3 ± 4.6 100
Radix Angelicae Sinensis crude polysaccharides 0.075 24.3 ± 8.1
1)1.1 ± 1.1
1)60
0.250 23.2±6.9
1) 1.3±1.2
1) 40
1)
YUEYUESHU CHONGJI 2.600 24.5 ± 8.4
1)0.9 ± 2.0
1)40
1)
Annotate: compare with model group,
1)P＜0.05.
The above results shows that disposable oral administration 0.075g/kg of Radix Angelicae Sinensis polysaccharide and 0.250g/kg cause that to chemical substance and thermostimulation the model of pain has obvious analgesic activity, and obviously suppresses " dysmenorrhea " induced mice writhing response and can effectively resist estrogen and bring out " dysmenorrhea " caused pain reaction.Radix Angelicae Sinensis polysaccharide also has analgesic effect more widely when low dose.4. test used medicine and material: it is Powdered, soluble in water that (1) medicine Radix Angelicae Sinensis polysaccharide is dark-brown, and it is 65.5% that phenol-sulfuric acid process is surveyed sugared content, mainly contains D-galactose and D-arabinose.As if YUEYUESHU CHONGJI is the western drugmaker in Henan Province; Aspirin, Huaiyin, Jiangsu pharmaceutical factory; Diethylstilbestrol injection, Shanghai the 9th pharmaceutical factory; Oxytocin inj, the biochemical-pharmaceutical factory, Nanjing.(2) key instrument GL-8402 type hot plate dolorimeter, medicine instrument plant in Ninghai, Zhejiang produces.(3) animal health Kunming mouse, except that " dysmenorrhea model " selects for use the ♂ mice as the negative control, the ♀ mice is all used in other experiment, and body weight 18 ± 1g is provided by Hubei Province's Experimental Animal Center, the animal quality certification number: 19-082.5. experimental technique: the influence of female mice writhing response due to (1) ASP Dichlorodiphenyl Acetate
Animal grouping and drug dose see Table 1, and the 1h per os is irritated stomach (ig) and is subjected to the reagent thing before the experiment, and model group waits the capacity normal saline.1h lumbar injection (ip) 1% acetic acid 0.2ml/ only observes in administration mouse writhing response latency and the 30min and turns round the body number of times after the administration, and calculates the protection percentage rate.(2) ASP is to the influence of hot plate method analgesic activity
With 55 ℃ of hot plates the ♀ mice is screened in advance, the qualified mice that is of foot reaction in 10～30 seconds, occurred licking.Experiment grouping and drug dose see Table 2.1h per os filling stomach (ig) is subjected to reagent, model group to wait the capacity normal saline before the experiment.For avoiding the influence of threshold of pain cyclic fluctuation, experiment all o'clock is carried out 25 ± 1 ℃ of room temperatures in the morning 8～10.The variation of the threshold of pain respectively organized in record, and calculate the threshold of pain and improve percentage rate.If sufficient reaction appears licking in the end in 60 seconds, the threshold of pain is counted 60 seconds.(3) ASP the Mouse Uterus spasm is brought out in diethylstilbestrol and oxytocin influence 1. " dysmenorrhea model " set up---the female mice hysterospasm is brought out in diethylstilbestrol and oxytocin
30 of ♀ mices.The optimum reaction condition of " dysmenorrhea model " due to---diethylstilbestrol and oxytocin---for groping to set up estrogen is divided into 3 groups (1 group, 2 groups, 3 groups) with mice, and adopts the different disposal mode.The dosage and the natural law of subcutaneous injection diethylstilbestrol see Table 4 before the zoopery.Experiment ip oxytocin on the same day, dosage sees Table 4.10 of alternative ♂ mices in contrast, with the specificity of dysmenorrhea model due to the reflection estrogen.Observe in each treated animal writhing response incubation period and the 30min and turn round the body number of times.If writhing response do not occur in the 30min, count 30min incubation period.
Writhing response---" dysmenorrhea model " due to the Mouse Uterus spasm brought out in table 4 diethylstilbestrol and oxytocin.n＝10， x±s
Diethylstilbestrol dosage and oxytocin writhing response
Grouping
(mg/ only * d) (u/ only) (min) number of times incubation period is (inferior/30min) incidence rate (%) for natural law
1 group 0.2 * 3 0.3 14.5 ± 5.4 6.2 ± 4.1 100
2 group 0.2 * 3 0.2 20.4 ± 12.7 5.7 ± 4.7 40
1)
3 group 0.2 * 2 0.3 29.4 ± 0.8
4)0.6 ± 0.8
1)20
3)
♂ mice matched group 0.2 * 3 0.3 30.0 ± 0.0
4)0.0 ± 0.0
2)0
3)Annotate: with 1 group of comparison,
1)P＜0.05,
2)P＜0.01,
3)P＜0.005,
4)P＜0.001.
By table 4 as seen, 1 group of mouse subcutaneous injection diethylstilbestrol 0.2mg/ only, 3d continuously, the 4th day ip oxytocin 0.3u/ only, turning round the body number of times is 6.2 ± 4.1 times, turning round the body incidence rate is 100%, the result shows that this is an optimum experimental condition.During with 1 group, writhing response appears in none example through the processing mode of estrogen for ♂ mice group.The sex difference that writhing response occurs in this model illustrate that estrogen acts on the target organ uterus and causes that excited the generation turn round body, the specificity of pointing out this modeling " dysmenorrhea " to react.2. diethylstilbestrol and oxytocin are brought out the influence of Mouse Uterus spasm
♀ mice group and drug dose see Table 3.Animal gives the estrogen mode with reference to 1 group of dysmenorrhea model optimum condition of being set up in the table 4, and the 1h per os is irritated stomach (ig) and is subjected to reagent thing or YUEYUESHU CHONGJI before the experiment, and model group waits the capacity normal saline.Observe in mouse writhing response latency and the 30min and turn round the body number of times.
Claims (2)
1. Radix Angelicae Sinensis polysaccharide is as the application of preparation analgesic.
2. purposes according to claim 1 is characterized in that: Radix Angelicae Sinensis polysaccharide is used to prepare capsule, tablet, granule and injection as effective ingredient.
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|---|---|---|---|
| CN 03118538 CN1433772A (en) | 2003-01-27 | 2003-01-27 | Use of angelica polysaccharide in preparation of analgesic |
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| CN 03118538 CN1433772A (en) | 2003-01-27 | 2003-01-27 | Use of angelica polysaccharide in preparation of analgesic |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104397699A (en) * | 2014-12-04 | 2015-03-11 | 郭士平 | Health care product for conditioning syndromes before and after menstruation and dysmenorrhea and preparation technology of health care product |
-
2003
- 2003-01-27 CN CN 03118538 patent/CN1433772A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104397699A (en) * | 2014-12-04 | 2015-03-11 | 郭士平 | Health care product for conditioning syndromes before and after menstruation and dysmenorrhea and preparation technology of health care product |
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