CN1431044A - Adsorbent based on montmorillonite for purifying blood and its preparing method - Google Patents
Adsorbent based on montmorillonite for purifying blood and its preparing method Download PDFInfo
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- CN1431044A CN1431044A CN 03115017 CN03115017A CN1431044A CN 1431044 A CN1431044 A CN 1431044A CN 03115017 CN03115017 CN 03115017 CN 03115017 A CN03115017 A CN 03115017A CN 1431044 A CN1431044 A CN 1431044A
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- montmorillonite
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Abstract
An adsorbent based on montmorillonite for purifying blood is a microsoftgel is prepared from purified montmorillonite, chitosan and gelatin through proportional mixing, spraying it in cold coagulating liquid, separation, water washing, sterilizing and baking. Its advantages are high biologic affinity, compability to blood and high adsorption power to toxin.
Description
Technical field
The present invention relates to a kind of blood-purifying adsorbing agent and preparation method thereof.
Background technology
Blood purification therapy (Blood Purification) is along with bio-medical engineering material and cellular elements biology and industrial expansion thereof and the new technology that fast development is got up, blood perfusion (Hemoperfusion as one of blood purification important component part, HP) indication is extensive, cost is lower, can promote the disease recovery from illness rapidly, save patient's life.Improvement along with adsorbent kind and performance, clinical practice is extensive day by day, by generally being used for handling anxious, severe medicine toxic poisoning, develop into treatment acute and chronic liver failure (artificial liver), acute or chronic renal failure (artificial kidney), thyroid crisis, schizophrenia, systemic loupus erythematosus, psoriasis, septicopyemia etc.
Blood perfusion is the important component part of biomedical engineering, be between medical science, material science and chemical engineering science, to have intercrossing, a marginal subject, its precise meaning is blood absorption, promptly by extracorporal circulatory system, by adsorbent units (perfusion device) with wide spectrum adsorpting detoxication effect or ligands specific, remove endogenous or exogenous morbid substance (adsorbate) in the blood, regulate the stable of the interior microenvironment of body, reach the purpose of alleviating clinical symptoms, cure diseases, the reduction death rate.Adsorbent commonly used at present mainly is active carbon and various resin, exist in varying degrees that adsorptive selectivity is bad, blood compatibility is poor, problem such as Hemodynamics instability in the adsorption process, can not satisfy the clinical demand of blood perfusion far away, research and development are efficient, high selectivity, can realize that the miniaturization of perfusion device, differential adsorbent are the important topics in blood purification field.
Summary of the invention
The purpose of this invention is to provide a kind of blood-purifying adsorbing agent based on montmorillonite and preparation method thereof.
Blood-purifying adsorbing agent based on montmorillonite of the present invention is a kind of montmorillonite micro-capsule with shitosan and the embedding of gelatin class bag.
Its preparation method comprises the steps:
1) with distilled water shitosan being configured to concentration is 1～8% the aqueous solution, adds and the equiponderant gelatin class of shitosan again, stirs also heating, dissolves fully until solid phase;
2) the na-montmorillonite powder that weight is equivalent to solution weight 15～35% joins in the above-mentioned solution, stirs and makes suspension, then 30～60 ℃ of constant temperature 2～6 hours, and stirs frequently;
3) pressurization of above-mentioned suspension is sprayed in the cold solidification liquid, be stirred to it is solidified;
4) centrifugal or isolated by filtration, washing, sterilization, oven dry below 80 ℃, packing gets final product.
Among the present invention, said gelatin class is one or more in gelatin, agar and the agarose.Solidification liquid can be to be 0.1～5% solution with one or more concentration that are configured in sodium phosphate trimer, sodium sulphate, sodium carbonate, natrium citricum and the NaOH.The sterilization of final products should be heated to 110～120 ℃ in steam, and keeps 20～30 minutes.
Among the present invention, said na-montmorillonite powder can be natural na-montmorillonite powder, also can be that the montmorillonite of other type is made according to the following steps:
1) will be the sodium salt of montmorillonite 5～15% through separating the montmorillonite and the weight of purifying, and add water and stir, make the suspension ore pulp of concentration about 10%;
2) under 40～100 ℃ of temperature, ion-exchange reactions at least six hours, during stir frequently;
3) precipitation is removed the particle greater than 2 microns, and the upper strata suspension obtains na-montmorillonite behind centrifugal or filtering means dehydration, and water cleans for several times, and dehydration again;
4) gains are ground to less than 300 orders in oven dry below 80 ℃, obtain water content less than 10% na-montmorillonite powder.Here, the used sodium salt of preparation na-montmorillonite can be sodium chloride, sodium nitrate, sodium sulphate.
Advantage of the present invention is:
1) raw materials used wide material sources and with low cost;
2) preparation technology is simple, required small investment;
3) montmorillonite is much higher than existing blood perfusion sorbing material spheric active carbon to the adsorption capacity of cationic poisonous substance, and is roughly suitable to the adsorption capacity and the spheric active carbon of anionic and electroneutral poisonous substance;
4) montmorillonite, shitosan and gelatin class are biological affine material, have good blood compatibility, and be safe in utilization.
The specific embodiment
The raw material that the present invention uses is the natural na-montmorillonite of purifying through separating, and has another name called sodium bentonite.Or the montmorillonite of other type, through sodium ion exchange, the na-montmorillonite of making.The purification technique of montmorillonite is well-known, and it comprises that suspension is made in pulverizing, making beating, the steps such as particle less than 2 microns are collected in classification.This highly purified montmorillonite is the clay mineral of a kind of light green color, light yellow tool layered crystal structure.Separate purification process and can select conventional mining equiment for use.
Bag embedding material selection shitosan and gelatin class mainly are because they have good bioaffinity and blood compatibility, and in addition, these two kinds of materials all obtain easily, and with low cost.
Select na-montmorillonite for use, or the montmorillonite of other type carried out sodium ion ion-exchange, be because na-montmorillonite has higher molecular activity, it has stronger selective adsorption capacity to each cationoid in the blood, and the sodium ion that discharges does not have obvious influence to the electrolyte balance and the blood chemistry composition of human body after the reaction.
Na-montmorillonite can be selected the conventional drying method for use, and baking temperature should be above 80 ℃; Also can the natural drying drying at the dry climate environment, again it is ground to granularity and is not more than 300 orders.
When configuration shitosan and gelatin class solution, preferably adopt the water-bath heating, to accelerate dissolution velocity.
The concentration of na-montmorillonite in shitosan and gelatin class solution preferably is controlled at about 25%, and overrich then is difficult to carry out next step operation, crosses rare performance that then can influence final products.
The outstanding liquid that mixes of na-montmorillonite, shitosan and gelatin class is sprayed in the solidification liquid, can select spraying apparatus routinely for use.The size of montmorillonite micro-capsule can be regulated by atomisation pressure and shower nozzle micro-pore diameter.
The sterilization of final products recommends to use Steam Heating, the decomposition in order to avoid montmorillonite dewaters because of heating-up temperature is too high.
Following example will further specify the present invention.
Embodiment 1
1) takes by weighing 1Kg through separating the ca-montmorillonite of purifying, add 9 liters in water, stir and make suspension.Add 150g NaCl, the back that stirs (needing 20 minutes approximately) is heated to about 60 ℃, ion-exchange reactions six hours, during stir frequently.Centrifugation then, the supernatant liquor that inclines adds 9 liters in water again, stirs, and continues centrifugation, and supernatant liquor inclines.Repeat 3～4 times, dehydration again, 80 ℃ of temperature oven dry are ground to less than 300 orders, get the na-montmorillonite powder.
2) add shitosan and each 40g of gelatin in 1 liter of distilled water, heating and stirring in 60 ℃ of water-baths are until dissolving fully.
3) take by weighing 300g na-montmorillonite powder, join in shitosan and the gelatin solution, stir, and in 60 ℃ of water-baths constant temperature 3 hours, during stir frequently.
4) concentration that adding prepares in advance in container is 1% NaOH solution, when stirring, hanging of na-montmorillonite, shitosan and gelatin is mixed liquid spray in the container.
5) continue gentle agitation after 20 minutes, centrifugal or isolated by filtration is washed to neutrality, moise-heat sterilization, and 80 ℃ of temperature oven dry, packing gets final product.
Embodiment 2
1) add shitosan and each 40g of agar in 1 liter of distilled water, heating and stirring in 50 ℃ of water-baths are until dissolving fully.
2) take by weighing the na-montmorillonite powder that 300g separate to purify, join in shitosan and the agar solution, stir, and in 50 ℃ of water-baths constant temperature 3 hours, during stir frequently.
3) concentration that adding prepares in advance in container is 1% sodium tripolyphosphate solution, when stirring, hanging of na-montmorillonite, shitosan and agar is mixed liquid spray in the container.
4) continue gentle agitation after 20 minutes, centrifugal or isolated by filtration is washed to neutrality, moise-heat sterilization, and 80 ℃ of temperature oven dry, packing gets final product.
Embodiment 3
1) add shitosan and each 40g of agar in 1 liter of distilled water, heating and stirring in 50 ℃ of water-baths are until dissolving fully.
2) take by weighing the na-montmorillonite powder that 300g separate to purify, join in shitosan and the agarose solution, stir, and in 50 ℃ of water-baths constant temperature 3 hours, during stir frequently.
3) concentration that adding prepares in advance in container is 1.5% metabisulfite solution, when stirring, hanging of na-montmorillonite, shitosan and agarose is mixed liquid spray in the container.
4) continue gentle agitation after 30 minutes, centrifugal or isolated by filtration is washed to neutrality, moise-heat sterilization, and 80 ℃ of temperature oven dry, packing gets final product.
Embodiment 4
1) add shitosan and each 30g of agar in 1 liter of distilled water, heating and stirring in 40 ℃ of water-baths are until dissolving fully.
2) take by weighing 250g na-montmorillonite powder, join in shitosan and the agar solution, stir, and in 40 ℃ of water-baths constant temperature 4 hours, during stir frequently.
3) concentration that adding prepares in advance in container is 1.5% sodium citrate solution, when stirring, hanging of na-montmorillonite, shitosan and agarose is mixed liquid spray in the container.
4) continue gentle agitation after 30 minutes, centrifugal or isolated by filtration is washed to neutrality, moise-heat sterilization, and 80 ℃ of temperature oven dry, packing gets final product.
Claims (9)
1. blood-purifying adsorbing agent based on montmorillonite is characterized in that it is a montmorillonite micro-capsule with shitosan and the embedding of gelatin class bag.
2. the blood-purifying adsorbing agent based on montmorillonite according to claim 1 is characterized in that said gelatin class is one or more in gelatin, agar and the agarose.
3. the preparation method of the blood-purifying adsorbing agent based on montmorillonite according to claim 1 is characterized in that its step is as follows:
1) with distilled water shitosan being configured to concentration is 1～8% the aqueous solution, adds and the equiponderant gelatin class of shitosan again, stirs also heating, dissolves fully until solid phase;
2) the na-montmorillonite powder that weight is equivalent to solution weight 15～35% joins in the above-mentioned solution, stirs and makes suspension, then 30～60 ℃ of constant temperature 2～6 hours, and stirs frequently;
3) pressurization of above-mentioned suspension is sprayed in the cold solidification liquid, be stirred to it is solidified;
4) centrifugal or isolated by filtration, washing, sterilization, oven dry below 80 ℃, packing gets final product.
4. the preparation method of the blood-purifying adsorbing agent based on montmorillonite according to claim 3 is characterized in that said gelatin class is one or more in gelatin, agar and the agarose.
5. the preparation method of the blood-purifying adsorbing agent based on montmorillonite according to claim 3 is characterized in that said na-montmorillonite powder is natural na-montmorillonite powder.
6. the preparation method of the blood-purifying adsorbing agent based on montmorillonite according to claim 3 is characterized in that said na-montmorillonite powder is to make according to the following steps:
1) will be the sodium salt of montmorillonite 5～15% through separating the montmorillonite and the weight of purifying, and add water and stir, make the suspension ore pulp of concentration about 10%;
2) under 40～100 ℃ of temperature, ion-exchange reactions at least six hours, during stir frequently;
3) precipitation is removed the particle greater than 2 microns, and the upper strata suspension obtains na-montmorillonite behind centrifugal or filtering means dehydration, and water cleans for several times, and dehydration again;
4) gains are ground to less than 300 orders in oven dry below 80 ℃, obtain water content less than 10% na-montmorillonite powder.
7. the preparation method of the blood-purifying adsorbing agent based on montmorillonite according to claim 6 is characterized in that said sodium salt is sodium chloride, sodium nitrate, sodium sulphate.
8. the preparation method of the blood-purifying adsorbing agent based on montmorillonite according to claim 3 is characterized in that said solidification liquid is is 0.1～5% solution with one or more concentration that are configured in sodium phosphate trimer, sodium sulphate, sodium carbonate, natrium citricum and the NaOH.
9. the preparation method of the blood-purifying adsorbing agent based on montmorillonite according to claim 3 is characterized in that said sterilization is meant to be heated to 110～120 ℃ in steam, and kept 20～30 minutes.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031150179A CN1169612C (en) | 2003-01-20 | 2003-01-20 | Adsorbent based on montmorillonite for purifying blood and its preparing method |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB031150179A CN1169612C (en) | 2003-01-20 | 2003-01-20 | Adsorbent based on montmorillonite for purifying blood and its preparing method |
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| CN1431044A true CN1431044A (en) | 2003-07-23 |
| CN1169612C CN1169612C (en) | 2004-10-06 |
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| CNB031150179A Expired - Fee Related CN1169612C (en) | 2003-01-20 | 2003-01-20 | Adsorbent based on montmorillonite for purifying blood and its preparing method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1314406C (en) * | 2003-04-17 | 2007-05-09 | 周良震 | Application of montmorillonite in pharmacy |
| CN100434463C (en) * | 2006-09-08 | 2008-11-19 | 武汉大学 | Chitosan quaternary ammonium salt/organic montmorillonite nano composite material and preparation method thereof |
| WO2017075946A1 (en) * | 2015-11-02 | 2017-05-11 | 李建中 | Adsorption filler for dialysate, and manufacturing method and use thereof |
| CN114904489A (en) * | 2022-05-31 | 2022-08-16 | 佛山市博新生物科技有限公司 | Blood perfusion adsorbent for removing uric acid and preparation method thereof |
| CN117696017A (en) * | 2024-02-05 | 2024-03-15 | 四川大学华西医院 | Blood purification adsorption modified material and preparation method thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100393369C (en) * | 2006-09-20 | 2008-06-11 | 天津大学 | Gelatin, montmorillonite and chitosan multi-porous tissue engineering supporting material and preparation method thereof |
-
2003
- 2003-01-20 CN CNB031150179A patent/CN1169612C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1314406C (en) * | 2003-04-17 | 2007-05-09 | 周良震 | Application of montmorillonite in pharmacy |
| CN100434463C (en) * | 2006-09-08 | 2008-11-19 | 武汉大学 | Chitosan quaternary ammonium salt/organic montmorillonite nano composite material and preparation method thereof |
| WO2017075946A1 (en) * | 2015-11-02 | 2017-05-11 | 李建中 | Adsorption filler for dialysate, and manufacturing method and use thereof |
| CN114904489A (en) * | 2022-05-31 | 2022-08-16 | 佛山市博新生物科技有限公司 | Blood perfusion adsorbent for removing uric acid and preparation method thereof |
| CN117696017A (en) * | 2024-02-05 | 2024-03-15 | 四川大学华西医院 | Blood purification adsorption modified material and preparation method thereof |
| CN117696017B (en) * | 2024-02-05 | 2024-05-07 | 四川大学华西医院 | Blood purification adsorption modified material and preparation method thereof |
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| Publication number | Publication date |
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| CN1169612C (en) | 2004-10-06 |
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