CN1420725A - Synergistic insect control - Google Patents
Synergistic insect control Download PDFInfo
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- CN1420725A CN1420725A CN00818240A CN00818240A CN1420725A CN 1420725 A CN1420725 A CN 1420725A CN 00818240 A CN00818240 A CN 00818240A CN 00818240 A CN00818240 A CN 00818240A CN 1420725 A CN1420725 A CN 1420725A
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- insect
- genetically modified
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- modified crops
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- 241000238631 Hexapoda Species 0.000 title claims abstract description 85
- 230000002195 synergetic effect Effects 0.000 title claims abstract description 8
- 238000000034 method Methods 0.000 claims abstract description 32
- 241000700605 Viruses Species 0.000 claims abstract description 27
- 244000037671 genetically modified crops Species 0.000 claims description 32
- 229920000742 Cotton Polymers 0.000 claims description 17
- 239000003053 toxin Substances 0.000 claims description 14
- 231100000765 toxin Toxicity 0.000 claims description 14
- 108700012359 toxins Proteins 0.000 claims description 14
- 241000256259 Noctuidae Species 0.000 claims description 6
- 241000193388 Bacillus thuringiensis Species 0.000 claims description 5
- 229940097012 bacillus thuringiensis Drugs 0.000 claims description 5
- 241000701443 Helicoverpa zea single nucleopolyhedrovirus Species 0.000 claims description 4
- 241000701366 unidentified nuclear polyhedrosis viruses Species 0.000 claims description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 208000003643 Callosities Diseases 0.000 claims description 2
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 2
- 244000299507 Gossypium hirsutum Species 0.000 claims 1
- 240000008042 Zea mays Species 0.000 claims 1
- 244000038559 crop plants Species 0.000 claims 1
- 230000009261 transgenic effect Effects 0.000 abstract description 4
- 101710182532 Toxin a Proteins 0.000 abstract 1
- 239000002919 insect venom Substances 0.000 abstract 1
- 241000219146 Gossypium Species 0.000 description 16
- 241000196324 Embryophyta Species 0.000 description 15
- 231100000419 toxicity Toxicity 0.000 description 12
- 230000001988 toxicity Effects 0.000 description 12
- 230000006378 damage Effects 0.000 description 8
- 230000001771 impaired effect Effects 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 239000000203 mixture Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 241000607479 Yersinia pestis Species 0.000 description 4
- 239000002917 insecticide Substances 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 239000007921 spray Substances 0.000 description 4
- 238000005507 spraying Methods 0.000 description 4
- 241000255967 Helicoverpa zea Species 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000003825 pressing Methods 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- 241001124076 Aphididae Species 0.000 description 2
- 241001148495 Cibotium barometz Species 0.000 description 2
- 241000241602 Gossypianthus Species 0.000 description 2
- 241000209149 Zea Species 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 235000019504 cigarettes Nutrition 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000008521 reorganization Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000004563 wettable powder Substances 0.000 description 2
- 241001367049 Autographa Species 0.000 description 1
- 241001203868 Autographa californica Species 0.000 description 1
- 108700003918 Bacillus Thuringiensis insecticidal crystal Proteins 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 241001147381 Helicoverpa armigera Species 0.000 description 1
- 241000256244 Heliothis virescens Species 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- 240000001140 Mimosa pudica Species 0.000 description 1
- 235000016462 Mimosa pudica Nutrition 0.000 description 1
- ABUGVBRDFWGJRD-CHOYNLESSA-N [9-[(2r,3r,4s,5r)-3,4-dihydroxy-5-methyloxolan-2-yl]-2-(2,4-dinitrophenyl)sulfanylpurin-6-yl] [hydroxy(phosphonooxy)phosphoryl] hydrogen phosphate Chemical compound O[C@@H]1[C@H](O)[C@@H](C)O[C@H]1N1C2=NC(SC=3C(=CC(=CC=3)[N+]([O-])=O)[N+]([O-])=O)=NC(OP(O)(=O)OP(O)(=O)OP(O)(O)=O)=C2N=C1 ABUGVBRDFWGJRD-CHOYNLESSA-N 0.000 description 1
- 238000000540 analysis of variance Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000006298 dechlorination reaction Methods 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 231100001228 moderately toxic Toxicity 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 238000010422 painting Methods 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/40—Viruses, e.g. bacteriophages
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
Abstract
There is provided a method for the synergistic control of insects which comprises applying to the locus of a transgenic crop which produces an insect toxin a synergistically effective amount of a recombinant insect virus containing a vector which is highly virulent to said insect.
Description
By chemical means control insect pest is that a kind of useful protection crops avoid attack of insect and the method for attacking the damage that is caused for a long time always.More recent, introduced the method that specificity is destroyed crop at the control insect of target insect, this method has been avoided and has been used relevant environment and the ecological hazard of conventional pesticides.A kind of use the in these methods can produce the insect specificity toxin, as the genetic modification crop of the Cry toxin of bacillus thuringiensis (Bacillus thuringiensis).Yet the crop of expression bacillus thuringiensis Cry toxin may show defense reaction in various degree to a large amount of lepidoptera pest species.For example, the cotton variety of expressing CryIA (c) is to tobacco budworm, be cigarette aphid noctuid (Heliothis virescens), high resistance is arranged, but the real noctuid of cotton bollworm paddy (Helicoverpa zea) only there are moderate resistance (J.H.Benedict etc., 1996, Journal ofEconomic Entomology, the 89th volume (1), the 230th page).
These class methods of another control insect are applying biological formulation example such as nucleopolyhedrosis virus (NPV) (United States Patent (USP) 4,668,511) or recombinant nuclear polyhedrosis virus (rNPV) (United States Patent (USP) 5,662,897 and United States Patent (USP) 5,858,353).Yet NPV and rNPV be at the virulence/effort levels of various insect species, can be according to the effectiveness of the toxin of the host range of the medicament that carries virus and insertion coded by said gene and different.For example, the real noctuid of insect species paddy is extremely sensitive to NPV and the rNPV of difference called after HzNPV and HzAaIT, but to autographa california (Autographa californica) NPV (AcNPV) or its rNPV, be the only medium susceptibility (Treacy etc. of tool of AcAaIT, 1999, Proceeding Beltwide Cotton Conf., the 1076-1083 page or leaf).Although will contain existing (the All and Treacy of description of combination that the recombinant nuclear polyhedrosis virus that the target insect species is had a carrier of poisoning toxicity is applied to the genetically modified crops strain, 1997, the 1294th page of Proceedings Beltwide Cotton Conf.), but these genetically modified crops and rNPV agent no matter separately still combine all not provide and prevent the required insect control level of crop loss on the commercial size.
Therefore, the purpose of this invention is to provide preventing crop destruction and the useful synergistic insect control method of economic loss that causes thus.
Another object of the present invention provides and strengthens the breaking-up that genetically modified crops defence attack of insect and invasion and attack are caused and the method for destruction.
A feature of the present invention is that the synergistic insect control and the crop protection method that are provided are special at the target insect species, and shows and have enhanced environmental and eco-compatibility, also provides the insect of commercial acceptable level to control and crop protection simultaneously.
From the following description and the appended claims, other purpose of the present invention and feature will be tangible for those skilled in the art.
The invention provides the method for synergistic insect control, comprise the recombinant insect virus that described insect is had supervirulent carrier that contains to genetically modified crops Zoned application cooperative effective quantity.
The present invention also provides the method that strengthens genetically modified crops defence attack of insect and attack the destruction of being caused.
Although the chemical method Pest Control is a kind of effective means of controlling important agricultural insect insect always, has introduced and more had the specific control method of target insect.Comprise that in these insect specificity methods use produces the genetically modified crops of insect toxins such as bacillus thuringiensis (Bt) toxin by hereditary change or uses natural viral such as nucleopolyhedrosis virus (NPV) or reorganization NPV (rNPV).Yet the genetically modified crops that produce the Bt toxin may show more not satisfied defence degree to the target insect.Similarly, the insect viruses of natural and reorganization when using as the independent method of control insect, the common effectiveness that also shows in various degree.
Although the target insect species had to containing being used in combination of the rNPV of moderately toxic carrier and genetically modified crops is existing describes, but, use described rNPV separately or when described rNPV is used with the genetically modified crops of genetically engineered generation insect toxins, do not obtaining gratifying result for commercial insect control.
Find now, give genetically modified crops, the genetically modified crops of preferred genetically engineered generation insect toxins (insecticide), use and contain the recombinant insect virus that the target insect species is had supervirulent carrier, demonstrate significant synergy (that is the insect that, obtained control effect can expect when using the toxicity recombinant insect virus separately or when using genetically modified crops separately insect control effect much higher).This synergy makes it possible to realize by biological means non-chemically the insect control of commercial useful level.And synergistic insect control method of the present invention makes it possible to achieve the effective resistance control that adapts with environment and ecological rational sustainable agriculture practical activity.
According to the inventive method, to the genetically modified crops kind, the genetically modified crops of preferred genetically engineered generation insect toxins, use the recombinant insect virus that the target insect species is had supervirulent carrier that contains of cooperative effective quantity, preferred recombinant nuclear polyhedrosis virus (rNPV) provides the Collaborative Control to insect pest.That is, use combination results that this toxicity recombinant insect virus causes insect composition extremely and kill insect effect (synergy) than what use killing of killing respectively that insect composition institute expectability arrives individually the insect effect is eager to excel to these genetically modified crops.
The recombinant insect virus that is applicable to strong toxicity carrier of containing of the inventive method comprises rNPV such as HzNPV, HzAzIT, EGTdel or their combination.
The genetically modified crops that are applicable to the generation insect toxins of the inventive method comprise corn and cotton strain (BTK system), for example the NuCotn 33B that expresses Bt
TM(a kind of Bollgard that passes through
TMTransformation event comes from Deltapine DP5415
TMThe transgenic cotton flower variety), or the transgenic corns kind is as expressing MON 810
TMThose of transformation event (YieldGard
TM, Monsanto Co.).
In the operation of reality, can be with the form of compositions formulated, for example wettable powder is applied to genetically modified crops with this toxicity recombinant insect virus, especially produces on zone, leaf or the stem of genetically modified crops of insect toxins, on the preferred leaf by hereditary change.Preferred preparation is described on the U.S. Patent Application Serial of submitting on June 9th, 1,998 09/094,279 common co-pending (incorporating this paper into as a reference).
The cooperative effective quantity of toxicity recombinant insect virus can be according to main condition, and for example the density of the insect-resistant degree of genetically modified crops, the selection of time of using, weather condition, mode of administration, insect colony, target crop species, target insect species wait and change.Usually, when with per hectare 1 * 10
10Individual occlusion body (OB/ha) is to 1 * 10
13OB/ha, preferred 5 * 10
10OB/ha to 12 * 10
11When the ratio of OB/ha is used the toxicity recombinant insect virus to genetically modified crops, the insect control that can obtain to work in coordination with.
In order to be beneficial to further understanding of the present invention, provide following examples, be mainly used in and illustrate its more specifically details.Except defined in the claim, should not think thus the present invention is construed as limiting.
In following examples, by Colby method (Colby, S.R., Weeds, 1967 (15), the 20-22 page or leaf) the definite pair of synergy that the approach insecticides make up, promptly, by each kills the result summation of insect composition gained to independent use, the product that deducts these two results then calculates expection (or prediction) result's (percentage of the insect of elimination) of this combination divided by the value after 100.This is described below by arithmetic mode, and wherein two approach combinations add that by component X component Y forms.
If during greater than the expected results that calculates from this formula, promptly there is synergy in the actual observation result.
In the present invention, the insect control percentage (not using external insecticide) that shows with respect to closely-related contrast crop genetically modified crops of the present invention can be expressed as X; The control percentage of recombinant insect virus of the present invention can be expressed as Y when being used to contrast on the crop.Can use the expection control percentage after aforementioned Colby formula calculates virus and genetically modified crops combination.If the observed result (actual control percentage) that is obtained by the combination of using the virus treated genetically modified crops is during greater than the expected results that calculates, then this combination has synergy.
Embodiment 1 estimates the Synergistic insecticidal effect of using the toxicity recombinant insect virus to genetically modified crops
In this estimated, the foliar spray that uses approaching typical case to run at the scene, cotton field spilt the test macro with the plant structure parameter.To (a) with 5 * 10
11OB/ha and 12 * 10
11Wettable powder (WP) preparation that the OB/ha ratio is used HzAaIT also (b) is expressed the cotton variety of bacillus thuringiensis CryIA (c) ' the insect effect of killing of NuCotn 33B ' is estimated, and makes comparisons with the combination of using conventional cotton variety ' DeltapineDP54151 '.
In being housed, 3.8 liters of plastic basin of commercial potting soil go out plant from seed culture.For purpose relatively, conventional Deltapine DP54151 cotton is included.About 1.5 months beginnings were used virus to cotton behind the cotton planting period.In the sealing chamber that the hanging and rotating type water jet is installed, spray plant.This water jet be furnished with three hollow taper nozzles (TX3, Spraying Systems, Wheaton, IL); A nozzle is provided for directly spraying on plant, and two nozzles are contained on the sag pipe of about 45 the side with the sprinkling plant.The bore of regulating this water jet is with by 3.5kg/cm
2Amount send 189 liters/ha; Use compressed air as spraying propeller.The rNPV insecticide of preparation is pressed 3.5L/ha together with taste stimulation agent Coax
TM(CCT Corp., Carlsbad CA) are suspended in the dechlorination water together.Sprayed plant 3 times every 7 days.On planar top, arrange potted plant vegetable lamb, repeat four times, and described planar top covers the dark water of 2cm of having an appointment and moves between plant to prevent larva with the design of completely random.Give each identical dosage of handling of two strain plants, from independently taking identical subsample the test.Be set at average every day of about 27 ℃ of minimum temperature in the environmental parameter of research process medium temperature chamber, and average every day, maximum temperature was about 32 ℃.
Spraying about 1 hour infection plant of after date with the real exigua larvae of the paddy of laboratory cultures at every turn.Use little painting brush that larva is placed on the leaf and square of the whole upper part of every strain vegetable lamb.Spray each after date of phase at three times and on every strain plant, place 30 fresh larvas that hatch altogether.Design is artificial mode of placing larva on plant, so as near natural distributed on cotton of the worm's ovum of this pest species and small grub (Farrar and Bradley, 1985, Environ.Entomol.).Use after date 7 days at the 3rd time, by writing down the impaired of every strain plant and not impaired square number, determine the efficient of these processing that cotton is carried out.By variance analysis (ANOVA, SAS Institute, 1989),, determine the significant difference between these processing with regard to of the damage of the real noctuid of paddy to cotton.Many arrangements test by Duncan (Duncan ' s multiple range tests) (DMRT; SAS Institute, 1989), the mean value of differentiating and processing.
Measured by the many arrangements test of Duncan, the mean value before the same letter does not have significant difference (P＜0.05; F[df5,18]=16.9); The percentage point data are carried out arcsine transformation to be used for analyzing.
7 days (the 3rd processing of 7DA3T=used back 7 days) estimates impaired and int square number on every strain plant after last using/infection period.The result
In this greenhouse research, infect the real exigua larvae of paddy weekly and cause untreated DP5415 cotton (susceptibility) to be subjected to significantly much bigger destruction, (53.0% and 20.8% impaired square is arranged respectively) than untreated NuCotn 33B (resistance).With 5 * 10
11OB/ha and 12 * 10
11Use HzAaIT on the consumption leaf of OB/ha and significantly reduced the destruction of insect two cotton varieties.When pressing 5 * 10 with HzAaIT
11OB/ha and 12 * 10
11When OB/ha handled, sensitive plant kind DP5415 produced 27.6% and 23.9% average impaired square number respectively.When pressing 5 * 10 with HzAaIT
11OB/ha and 12 * 10
11When OB/ha handled, resistance plant kind NuCotn33B produced 8.8% and 5.0% average impaired square number respectively.Data show is in table 1.
As what can from the data of table 1, see, with insect the destruction of untreated genetically modified crops is compared, with 12 * 10
11The consumption of OB/ha is used toxicity recombinant insect virus (HzAaIT) to genetically modified crops (NuCotn33B) leaf portion makes the destructiveness of insect descend 4.2 times, and compares with untreated susceptibility crop, with 12 * 10
11The consumption of OB/ha is used described toxicity recombinant insect virus to susceptibility crop (DP5415) only makes the destructiveness of insect descend 2.2 times.Therefore, the combined method that the toxicity recombinant insect virus is applied to genetically modified crops makes the destructiveness of insect, and is lower about 2 times than the insect destructiveness of using this toxicity recombinant insect virus separately or use genetically modified crops institute expectability to arrive separately.
Table 1 is by cotton bollworm, cigarette aphid noctuid on the administered recombinant nucleopolyhedrosis virus HzNPV of leaf portion (Egtdel)/DA26-ADK-AaIT (HaZaIT) control routine and the transgenic cotton flower variety
1Many arrangements of pressing Duncan are determined, and the mean value before the same letter does not have significant difference (P＜0.05; F[df 5,18]=16.9); The percentage point data are carried out arcsine transformation to be used for analyzing.
2 *Synergy=observed value＞desired value
| Cotton variety ﹠ leaf is handled | The impaired square mean value of % (± SD) 7DA3T | The % contrast 2 | |
| Observed value | Desired value | ||
| DP5415 | |||
| HZAaIT 5×10 11OB/ha | 27.6b (±7.5) | ????47.9 | ????NA |
| HZAaIT 12×10 11OB/ha | 23.9b (±6.8) | ????54.9 | ????NA |
| Untreated | 53.0b (±9.4) | ????NA | ????NA |
| NuCotn?33B | |||
| HZAaIT | 8.8c | ????83.4 * | ????79.6 |
| ??5×10 11OB/ha | ????(±5.6) | ||
| ??HZAaIT ??12×10 11OB/ha | ????5.0c ????(±3.4) | ????90.6 * | ????82.3 |
| Do not spray | ????20.8b ????(±10.4) | ????60.8 | ????NA |
Claims (10)
1. the method for synergistic insect control comprises the recombinant insect virus that described insect is had supervirulent carrier that contains of using zone from cooperative effective quantity to the genetically modified crops that produce insect toxins, leaf or stem.
2. according to the process of claim 1 wherein that described recombinant virus is a recombinant nuclear polyhedrosis virus.
3. according to the method for claim 2, wherein said recombinant virus is HzNPV, HzAIT, EGTdel or their combination.
4. according to the process of claim 1 wherein that described genetically modified crops are crop plants of genetically engineered expression bacillus thuringiensis toxin.
5. according to the method for claim 4, wherein said genetically modified crops are corns.
6. according to the method for claim 4, wherein said genetically modified crops are cottons.
7. according to the method for claim 6, wherein said crop is NuCotn 33B.
8. according to the method for claim 2, the cooperative effective quantity of wherein said recombinant insect virus is 1 * 10
10OB/ha to 1 * 10
13OB/ha.
9. method according to Claim 8, wherein insect is a lepidopterous insects.
10. according to the method for claim 9, wherein insect is the real noctuid of paddy.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US47950000A | 2000-01-07 | 2000-01-07 | |
| US09/479,500 | 2000-01-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1420725A true CN1420725A (en) | 2003-05-28 |
Family
ID=23904281
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN00818240A Pending CN1420725A (en) | 2000-01-07 | 2000-12-21 | Synergistic insect control |
Country Status (15)
| Country | Link |
|---|---|
| EP (1) | EP1244360A2 (en) |
| JP (1) | JP2003519638A (en) |
| KR (1) | KR20020065923A (en) |
| CN (1) | CN1420725A (en) |
| AR (1) | AR026806A1 (en) |
| AU (1) | AU3727001A (en) |
| BR (1) | BR0016924A (en) |
| CA (1) | CA2396562A1 (en) |
| CZ (1) | CZ20022301A3 (en) |
| EG (1) | EG22209A (en) |
| HU (1) | HUP0203815A2 (en) |
| IL (1) | IL150379A0 (en) |
| PL (1) | PL357639A1 (en) |
| SK (1) | SK9672002A3 (en) |
| WO (1) | WO2001050865A2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102981001B (en) * | 2012-11-22 | 2014-11-12 | 中国农业科学院植物保护研究所 | Method for evaluating cotton bollworm resistance control effect |
-
2000
- 2000-12-21 CZ CZ20022301A patent/CZ20022301A3/en unknown
- 2000-12-21 CA CA002396562A patent/CA2396562A1/en not_active Abandoned
- 2000-12-21 AU AU37270/01A patent/AU3727001A/en not_active Abandoned
- 2000-12-21 EP EP00991987A patent/EP1244360A2/en not_active Withdrawn
- 2000-12-21 JP JP2001551300A patent/JP2003519638A/en not_active Withdrawn
- 2000-12-21 HU HU0203815A patent/HUP0203815A2/en unknown
- 2000-12-21 IL IL15037900A patent/IL150379A0/en unknown
- 2000-12-21 BR BR0016924-2A patent/BR0016924A/en not_active Application Discontinuation
- 2000-12-21 SK SK967-2002A patent/SK9672002A3/en unknown
- 2000-12-21 WO PCT/EP2000/013094 patent/WO2001050865A2/en not_active Application Discontinuation
- 2000-12-21 PL PL00357639A patent/PL357639A1/en not_active Application Discontinuation
- 2000-12-21 CN CN00818240A patent/CN1420725A/en active Pending
- 2000-12-21 KR KR1020027008779A patent/KR20020065923A/en not_active Application Discontinuation
- 2000-12-30 EG EG20001602A patent/EG22209A/en active
-
2001
- 2001-01-05 AR ARP010100058A patent/AR026806A1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EG22209A (en) | 2002-10-31 |
| CA2396562A1 (en) | 2001-07-19 |
| WO2001050865A3 (en) | 2002-05-23 |
| SK9672002A3 (en) | 2003-01-09 |
| WO2001050865A2 (en) | 2001-07-19 |
| HUP0203815A2 (en) | 2003-04-28 |
| PL357639A1 (en) | 2004-07-26 |
| KR20020065923A (en) | 2002-08-14 |
| IL150379A0 (en) | 2002-12-01 |
| BR0016924A (en) | 2002-11-12 |
| AR026806A1 (en) | 2003-02-26 |
| EP1244360A2 (en) | 2002-10-02 |
| AU3727001A (en) | 2001-07-24 |
| JP2003519638A (en) | 2003-06-24 |
| CZ20022301A3 (en) | 2002-10-16 |
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