CN1415381A - Method for eliminating pollution of nucleic acid - Google Patents
Method for eliminating pollution of nucleic acid Download PDFInfo
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- CN1415381A CN1415381A CN 02133044 CN02133044A CN1415381A CN 1415381 A CN1415381 A CN 1415381A CN 02133044 CN02133044 CN 02133044 CN 02133044 A CN02133044 A CN 02133044A CN 1415381 A CN1415381 A CN 1415381A
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- acid
- nucleic acid
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Abstract
A method for eliminating nucleic acid pollution features that the instrument, utensils, consumable materials and lab are fumigated by the vapor of volatile acid to eliminte the polluted DNA and RNA, the clean air is used to carry out said volatile acid, and the air is cleaned by acid absorber for using it cyclically.
Description
The present invention relates to biology field, specifically is a kind of precautionary measures of pollution of nucleic acid.
PCR (polymerase chain reaction, polymerase chain reaction) and deutero-RT-PCR (reverse transcription-PCR, RT-polymerase chain reaction) thereof are extremely sensitive molecular biology experiment means.Can detect one nucleic acid molecules in theory, and with low cost, and its superiority is not sayed and is resided.But meanwhile, the atomic little pollution of nucleic acid of existence such as instrument, vessel, consumptive material, experiment place all may cause false positive results.And PCR, RT-PCR etc. itself depend on the purpose nucleic acid fragment are carried out billions of times of amplifications, and this has further increased contamination of heavy.
The existing method of eliminating pollution of nucleic acid mainly is ultraviolet irradiation, acid soak method and a large amount of clear water infinite dilution method.The shortcoming of ultraviolet irradiation is that ultraviolet straightline propagation has been doomed to stay a large amount of dead angles.The shortcoming of acid soak method is serious to metal erosion; Must be aided with washing during end, and washing may bring new pollution of nucleic acid; Pollute the environment behind the discharge of wastewater; Whole process is difficult to realize automatization.The shortcoming of a large amount of clear water infinite dilution methods is waste water resources; And be difficult to guarantee thoroughly remove pollution of nucleic acid.At present, China forbids PCR method is used for clinical diagnosis, and it is one of the main reasons that pollution problem is difficult to solve.
The objective of the invention is to effective eliminating pollution of nucleic acid, thereby guarantee the reliability of PCR, RT-PCR, in situ hybridization equimolecular biological experimental method, for the clinical practice of ratifying in the future PCR lays the first stone.
The objective of the invention is to adopt following technical proposals to realize:
At first, utilize steam stifling experimental apparatus, vessel, consumptive material, experiment place etc. under normal pressure, room temperature or hot a little (≤100 ℃) condition of volatile acid (hydrochloric acid, nitric acid, acetic acid etc.), utterly destroy the DNA and the RNA of pollution.I found through experiments: 37.5% hydrochloric acid full closed the steam effect 15 seconds or 65% nitric acid full closed the steam effect 30 seconds or the saturated vapor effect of glacial acetic acid can be utterly destroyed the DNA and the RNA of pollution in 4 hours under normal pressure, the room temperature.
Then, be blown into through ultraviolet radiation and the pure air that filter to obtain or nitrogen thoroughly take away acid stifling after experimental apparatus, vessel, consumptive material, residual volatile acid and the steam thereof in experiment place.This step also can or be carried out under heat (≤100 ℃) condition a little in normal pressure, room temperature.
At last, utilize water absorption method or alkali absorb method (soda lime etc.) to handle the air of band acid, make its recycling, or repeatedly deacidification, emptying behind the assurance anacidity.
Can directly coming into operation in experimental apparatus after above processing, vessel, consumptive material, experiment place etc., need not further wash.When washing if necessary, can wash earlier and utilize step eliminating pollution of nucleic acid of the present invention then.
The invention has the advantages that:
1. acid can arrive each corner, does not stay the dead angle.
2. eliminating pollution of nucleic acid efficient height.
3. applied widely, less demanding for fumigated article heat-resisting quantity, corrosion resistance etc.
4. be applicable to the removing of pollution of nucleic acid such as large-scale instrument, vessel, consumptive material, room, also be applicable to miniature biochip.
Step simple, avoid the secondary pollution of nucleic acid.
6. easily be automated.
7. with low cost.
Claims (3)
1. the method for an eliminating pollution of nucleic acid: it is characterized in that utilizing volatile acid (hydrochloric acid, nitric acid, acetic acid etc.) to fumigate experimental apparatus, vessel, consumptive material, experiment place etc., utterly destroy the DNA and the RNA of pollution.
2. afterwards utilize pure air or nitrogen that volatile acid is taken away according to described the fumigating of claim 1.
3. air that has a volatile acid according to claim 2 or nitrogen recycle after removing acid treatment or emptying after thoroughly deacidifying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021330441A CN100408104C (en) | 2002-09-26 | 2002-09-26 | Method for eliminating pollution of nucleic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021330441A CN100408104C (en) | 2002-09-26 | 2002-09-26 | Method for eliminating pollution of nucleic acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1415381A true CN1415381A (en) | 2003-05-07 |
| CN100408104C CN100408104C (en) | 2008-08-06 |
Family
ID=4747039
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021330441A Expired - Fee Related CN100408104C (en) | 2002-09-26 | 2002-09-26 | Method for eliminating pollution of nucleic acid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100408104C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104894277A (en) * | 2015-06-19 | 2015-09-09 | 许昌学院 | Method for preventing aerosol pollution caused by nucleic acid isothermal amplification product |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3071972D1 (en) * | 1979-11-02 | 1987-06-25 | Theurer Karl | Process for concentration of tumor-inhibiting substances |
| US5527508A (en) * | 1992-11-12 | 1996-06-18 | American Sterilizer Company | Method of enhanced penetration of low vapor pressure chemical vapor sterilants during sterilization |
| CN2321442Y (en) * | 1997-11-08 | 1999-06-02 | 林章建 | Heating device for evaporating acetic acid solution |
| DE19801209A1 (en) * | 1998-01-15 | 1999-07-22 | Schaefer Thomas Dr | Use of vinegar to kill bacteria |
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2002
- 2002-09-26 CN CNB021330441A patent/CN100408104C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104894277A (en) * | 2015-06-19 | 2015-09-09 | 许昌学院 | Method for preventing aerosol pollution caused by nucleic acid isothermal amplification product |
| CN104894277B (en) * | 2015-06-19 | 2020-09-11 | 许昌学院 | Method for preventing aerosol pollution of isothermal amplification product of nucleic acid |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100408104C (en) | 2008-08-06 |
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| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080806 Termination date: 20091026 |