CN1414383A - Bidirectional plate gel electrophoresis device - Google Patents
Bidirectional plate gel electrophoresis device Download PDFInfo
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- CN1414383A CN1414383A CN 02136744 CN02136744A CN1414383A CN 1414383 A CN1414383 A CN 1414383A CN 02136744 CN02136744 CN 02136744 CN 02136744 A CN02136744 A CN 02136744A CN 1414383 A CN1414383 A CN 1414383A
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Abstract
A two-way flat plate gel electrophoresis device includes an upper and a lower troughs. The upper trough contains the H-type bracket composed of two side walls and bottom plate. The circular hole and the channel for the electrode plug board are set up on the two side walls. There are the front and rear walls set up in the upper through. The front wall is a flat plate with double notch in upper and lower edges. The rear wall is a rectangular plate. The device also includes following three types of plates: the parallel double-concave plate, the vertical double-concave plate and the plate with three notches. One parallel double concave plate is put on the upper trough. The spaced bars are positioned on the left and right side of the other parallel double-concave plate being covered with glass and fixed by the clamp.
Description
The present invention relates to a kind of new design that is used for the electrophoretic apparatus in biological chemistry, biophysics, molecular biology, cell biology, biotech medicine product and proteomics field.Specifically, be the multi-usage electrophoretic apparatus of efficient, easy, the economical bidirectional plate polyacrylamide of a kind of self-supporting, Ago-Gel.
Polyacrylamide gel electrophoresis (PAGE) is that a kind of biological chemistry, biophysics, molecular biology, cell biology, molecular genetics, molecular medicine, the biotech medicine product that grow up nineteen fifties are learned the zone electrophoresis technology in the research experiment.Dull and stereotyped polyacrylamide gel electrophoresis device both domestic and external generally only is used for biomacromolecule protein, polypeptide and foranalysis of nucleic acids.In the past, be the plate electrophoresis of preparation purpose, glue is located, cut to adopt of people to the purpose component district band on the gel slab pre-" dye and show " more, and immersion then or energising wash-out make the purpose component enter solution; At last, concentrated, dry.Also someone's gel of haveing no alternative but to contain destination protein downcuts, grinds, is directly used in sensitized animal.
The present invention aims to provide a kind of multi-usage gel disk electrophoresis device.Enforcement of the present invention both can have been made vertical panel polyacrylamide gel (and Ago-Gel) analytical electrophoresis device, preparation electrophoretic apparatus, dielectrophoresis device, gel electroelution device, can be used as the electrodialysis device again.
So far, with regard to tabular gel electrophoresis apparatus, do not see other identical imagination and products as yet.For example, the V16 of Shanghai City lean factory, the PAGE device of the biochemical scientific research industry and trade of Xuhui District of Shanghai China swimming institute, the prompt MV-1 that steps in Daliang City pacifies the Mighty Small of agate West Asia company (Amersham Sciences) and He Fu company (Hoefer)
TMThe little 3 type gel slab electrophoresis tanks of the nucleic acid-protein of SE 245 and Bio Rad Laboratories (BioRad) (Protean mini 3 Cell).All belong to slab gel analytical electrophoresis device.A most tool special purpose.The little 2-D type electrophoresis tank (Mini2-D Electrophoresis Cell) of Bole company (BioRad) is two-way analytical electrophoresis device.Full glue elution instrument of Bole company (BioRad) (Whole Gel Eluter) and small-sized full glue elution instrument (Mini Whole Gel Eluter) are simple electroelution devices.491 preparation facilities of Bole company (BioRad) (Model Prep Cell) and small-sized preparation facilities (Model Mini PrepCell) are single column casing shape gel electrophoresis devices.This preparation electrophoretic apparatus requires height to the input of fund and equipment, uses quite to be not easy.
When the invention process inversion slab gel prepares electrophoretic apparatus, use the flat board of counterpart parallel double recess, implement to turn to the preparation electrophoresis.Have test condition and determine easily, reduced that the difficulty of exploring, heat exchanger effectiveness height, electrophoresis process are fast, good separating effect, easy, economic, practical characteristics.When being engaged in the research of purifying, preparation biomacromolecule, can make purpose component Direct Transfer under electric field action enter solution.Improve efficient, yield, saved the cost of fundamental research and applied research experiment, shortened the time.
During the two-way analytical electrophoresis device of the invention process vertical panel polyacrylamide gel, carry offset plate, implement second to electrophoresis with accessory single notching or vertical concave-concave mouth.Have easy and simple to handlely, especially use vertical concave-concave mouth and carry offset plate, can make purpose bar road keep somewhere original position, do two, can receive the effect of getting twice the result with half the effort to electrophoresis first after electrophoresis.
The present invention is achieved in that as a kind of multi-usage plate gel electrophoresis device and comprises groove under the electrophoresis that is provided with conventional electrical appliance socket and platinum electrode silk by top, two sides (being called for short groove down); And the electrophoresis adjusted direction that matches with this time groove can stand upright on the last groove of this time groove again.Should go up groove by two side and base plate, be all rectangular panel blocks by constitute H shape supporting structure.The dull and stereotyped permanent bonding upward groove integral body that forms of H shape support and forward and backward wall.This two side is provided with and contains plug circular hole and embedded electrode device socket plate slot.Should go up the groove front face is the flat board that recess is all established in a upper and lower end, and two recesses of this flat board are the trend that is parallel to each other (the parallel concave-concave flat board of groove on the abbreviation).The outline that should go up the parallel concave-concave flat board of groove is rectangular; Its vertical edges is contour with the vertical edges of the sidewall that should go up groove H shape support.The rear wall that should go up groove is a rectangular flat.
The present invention is during as conventional gel analysis electrophoretic apparatus, and bar between left and right sides vertical edges of the parallel concave-concave flat board of groove is placed on this is gone up coated with glass plate.The sub-clamping of two retable feed collets about mat.Stand on the organic glass shallow slot then.Unload the organic glass shallow slot behind the encapsulating, in groove under the groove shift discharge swimming on the lotus glue.At this moment, the hole of the last groove shutoff of jumping a queue; The hole of last groove hypomere is the uncork state.Movable embedded electrode device socket plate is inserted the conduit of going up groove.
The concave-concave flat board of recess is all opened on the upper and lower limit that the present invention is provided with for the enforcement gel electrophoresis, and recess is runs parallel mutually up and down, parallel concave-concave writing board shape with last groove and the identical flat board of area.The bag filter or other semi-permeable diaphragms that size are enough to cover dull and stereotyped recess place the dull and stereotyped recess, the concave-concave plate stack of this peripheral hardware is placed on it of the parallel concave-concave of groove; This dull and stereotyped left and right sides vertical edges balance be provided with thickness reach some millimeters between bar, go up coated with glass plate.Put into organic glass encapsulating shallow slot then, take off the encapsulating shallow slot behind the encapsulating, will go up groove and transfer to down in the groove.The shutoff of jumping a queue of the hole of last groove; The hole at the following position of last bottom land is the uncork state.Movable embedded electrode device socket plate is inserted the conduit of going up groove.
Use can be extracted movable embedded electrode device socket plate out from the conduit of last groove.After the inversion of whole upward groove, movable embedded electrode device socket plate is inserted the conduit of going up groove.The shutoff of jumping a queue of the hole of last groove; The hole at the following position of last bottom land is the uncork state.To finish the overall process of preparation electrophoresis.
The present invention relates to implement a concave-concave flat board that can be stacked and placed on the parallel concave-concave flat board of groove of dielectrophoresis device, this dull and stereotyped feature is that two recesses are capwise (being called for short vertical concave-concave flat board) mutually; This vertical concave-concave plate stack places the parallel concave-concave flat board of groove, and bar between its left and right sides vertical edges is placed is specially joined the recess that vertical edges is mended in a bar embedding with a slice, on cover glass plate, use the clip clamping then, stand on the organic glass shallow slot.Unload the organic glass groove behind the encapsulating.Groove under the last groove dislocation electrophoresis.The shutoff of jumping a queue of the hole of last groove; The hole at the following position of last bottom land is the uncork state.Movable embedded electrode device socket plate is inserted the conduit of going up groove.
The present invention is during as the gel analysis electrophoretic apparatus, and bar between left and right sides vertical edges of the parallel concave-concave flat board of groove is placed on this is gone up coated with glass plate.The sub-clamping of two retable feed collets stands on the organic glass glue-filling slot then about mat.Unload the organic glass groove behind the encapsulating, with groove under the groove shift discharge swimming on the lotus glue.The shutoff of jumping a queue of the hole of last groove; The hole at the following position of last bottom land is the uncork state.Movable embedded electrode device socket plate is inserted the conduit of going up groove.
The present invention is removably single recessed flat board as the one flat plate of implementing the dielectrophoresis device, is stacked and placed on the parallel concave-concave flat board of groove.The limit, the left and right sides of the recessed flat board of this list symmetry place thickness be about 1 millimeter between bar, go up coated with glass plate, the sub-clamping of two retable feed collets stands on the organic glass glue-filling slot then about mat.Unload the organic glass groove behind the encapsulating, in groove under the last groove shift discharge swimming of lotus glue.The shutoff of jumping a queue of the hole of last groove; The hole at the following position of last bottom land is the uncork state.Movable embedded electrode device socket plate is inserted the conduit of going up groove.
Implement another concave-concave flat board that can load and unload, be stacked and placed on the parallel concave-concave flat board of groove of dielectrophoresis device, two recesses of this flat board are capwise (being called for short vertical concave-concave flat board) mutually; After this vertical concave-concave plate stack places the parallel concave-concave flat board of groove, bar between its left and right sides vertical edges is placed, the recess on the vertical edges, embedding is mended a slice and is specially joined a bar, on cover glass plate, use the clip clamping, stand on the organic glass shallow slot.Unload the organic glass groove behind the encapsulating.Groove under the last groove dislocation electrophoresis.The shutoff of jumping a queue of the hole of last groove; The hole at the following position of last bottom land is the uncork state.Movable embedded electrode device socket plate is inserted the conduit of going up groove.
Implement one three a recessed flat board that can load and unload, be stacked and placed on the parallel concave-concave flat board of groove of dielectrophoresis device, three recesses of this flat board are ∩ trend (being called for short three recessed flat boards); After this three recessed plate stack places the parallel concave-concave flat board of groove, bar between its left and right sides vertical edges is placed, the recess on the vertical edges, embedding is mended a slice and is specially joined a bar, on cover glass plate, use the clip clamping, stand on the organic glass shallow slot.Unload the organic glass groove behind the encapsulating.Groove under the last groove dislocation electrophoresis.The shutoff of jumping a queue of the hole of last groove; The hole at the following position of last bottom land is the uncork state.Movable embedded electrode device socket plate is inserted the conduit of going up groove.
Further, get the recessed or concave-concave plate stack of the list of a peripheral hardware when enforcement of the present invention both can have been made vertical panel polyacrylamide gel wash-out device arbitrarily and place the parallel concave-concave flat board of groove, put a slice semi-permeable diaphragm (as bag filter) between two flat boards, dull and stereotyped side folded outside this is set up bar between some, again coated with glass plate, the clip clamping stands on the organic glass glue-filling slot then.Unload the organic glass groove behind the encapsulating, in groove under the last groove shift discharge swimming of lotus glue.The shutoff of jumping a queue of the hole of last groove; The hole of hypomere is the uncork state.Movable embedded electrode device socket plate is inserted the conduit of going up groove can carry out gel electroelution or electrodialysis.
Generally speaking, enforcement of the present invention both can have been made the vertical panel polyacrylamide gel and prepare electrophoretic apparatus, two-way analytical electrophoresis device, conventional analysis electrophoretic apparatus, gel electroelution device, can be used as the electrodialysis device again.
Accompanying drawing of the present invention is summarized as follows:
The schematic appearance of Fig. 1, the two-way flat gel electrophoresis apparatus of embodiments of the invention.
The perspective exploded view of the preparation electrophoretic apparatus of Fig. 2, embodiment of the invention bidirectional plate gel electrophoresis device.
The perspective exploded view of Fig. 3, two-way flat gel electrophoresis apparatus conventional analysis electrophoretic apparatus of the invention process.
The perspective exploded view of Fig. 4, the two-way analytical electrophoresis device of two-way flat gel electrophoresis apparatus of the invention process.
Another perspective exploded view of the two-way analytical electrophoresis device of the enforcement of Fig. 5, two-way flat gel electrophoresis apparatus of the invention process.
The H shape shelf structure synoptic diagram of groove on Fig. 6, the bidirectional plate gel analysis electrophoretic apparatus of the invention process.
Fig. 7, slab gel two-way system back-up of the invention process analyse the last groove H shape frame of electrophoretic apparatus bonding front and rear wall dull and stereotyped constitute go up groove body synoptic diagram.
The set last groove front and rear wall of the bidirectional plate gel electrophoresis device of Fig. 8, enforcement of the present invention reaches and the folded mutually dull and stereotyped synoptic diagram of last groove.
8-1, the last parallel concave-concave flat board of groove (antetheca); Fig. 8-2, last groove rear wall flat board;
Fig. 8-3, single recessed flat board; 8-4, parallel concave-concave flat board;
Fig. 8-5, vertical concave-concave flat board; Fig. 8-6, three recessed flat boards.
Below, provide better embodiment of the present invention according to Fig. 1~Fig. 8.This is not restriction enforcement of the present invention.Structure member of the present invention divides three parts, and as listed below: first comprises the ccontaining cushion block 11,12 of laying groove of 1 liang of side bottom of groove down for implementing conventional polyacrylamide gel electrophoresis device; Upper end receptacle 13,14 of following groove both sides and platinum filament 15.The ingredient plate 20 that comprises groove 2 simultaneously is the end; 21,22 are sidewall formation H shape support (accompanying drawing 2,6).Fig. 2,6 can see the conduit 211,221 that movable embedded electrode device socket plate inserts.Movable embedded electrode device socket plate 23, electrode socket 231, platinum filament 233.Fig. 6 is also shown in the groove two side and establishes some equal aperture holes and have 211,212 on top, 221,222; (214,215,224,225) are arranged at the bottom; The hole on top, last groove two side is all established some corresponding general sealing-plugs (216,217,226,227).Fig. 2 is illustrated in when implementing conventional plate electrophoresis, respectively puts a bar (26,27) at the dual-side of the parallel concave-concave flat board of last groove (Fig. 7), goes up coated with glass plate 28 usefulness clip (29,30) clamping.Stand on organic glass glue-filling slot 40 then.Unload the organic glass groove behind the encapsulating, with groove 1 under the last groove shift discharge swimming of lotus glue.216,217,226,227 shutoff of jumping a queue respectively of the hole 212,213,222,223 of last groove; The hole of hypomere is the uncork state.Movable embedded electrode device socket plate 23 is inserted the conduit of going up groove.
Second portion comprises the ccontaining cushion block 11,12 of laying groove of 1 liang of side bottom of groove down for implementing conventional polyacrylamide gel electrophoresis device; Upper end receptacle 13,14 of following groove both sides and platinum filament 15.The ingredient plate 20 that comprises groove 2 simultaneously is the end; 21,22 are sidewall formation H shape support (accompanying drawing 2,6).Fig. 2,6 can see the conduit 211,221 that movable embedded electrode device socket plate inserts.Movable embedded electrode device socket plate 23, electrode socket 231, platinum filament 233.Fig. 6 is also shown in the groove two side and establishes some equal aperture holes and have 211,212 on top, 221,222; (214,215,224,225) are arranged at the bottom; The hole on top, last groove two side is all established some corresponding general sealing-plugs (216,217,226,227).The parallel concave-concave flat board of last groove (Fig. 7) dual-side is respectively put bar between 1 (26,27), goes up coated with glass plate 28, with clip (29,30) clamping.Stand on organic glass glue-filling slot 40 then.Unload the organic glass groove behind the encapsulating, with groove 1 under the last groove shift discharge swimming of lotus glue.216,217,226,227 shutoff of jumping a queue respectively of the hole 212,213,222,223 of last groove; The hole of hypomere is the uncork state.Movable embedded electrode device socket plate 23 is inserted the conduit of going up groove.
Third part is illustrated in figure 4 as the two-way polyacrylamide gel analytical electrophoresis device of enforcement.Comprise the ccontaining cushion block 11,12 of laying groove of 1 liang of side bottom of groove down; Upper end receptacle 13,14 of following groove both sides and platinum filament 15.The ingredient plate 20 that comprises groove 2 simultaneously is the end; 21,22 are sidewall formation H shape support (accompanying drawing 2,6).Fig. 2,6 can see the conduit 211,221 that movable embedded electrode device socket plate inserts.Movable embedded electrode device socket plate 23, electrode socket 231, platinum filament 233.Fig. 6 is also shown in the groove two side and establishes some equal aperture holes and have 211,212 on top, 221,222; (214,215,224,225) are arranged at the bottom; The hole on top, last groove two side is all established some corresponding general sealing-plugs (216,217,226,227).At the stacked vertical concave-concave flat board of the parallel concave-concave flat board of last groove (Fig. 7) (Fig. 8-5), dual-side is respectively put bar between 1 (26,27) bar between its left and right sides vertical edges is placed, recess on the vertical edges, embedding are mended a slice and are specially joined bar between weak point (figure omits), go up coated with glass plate 28, with clip (29,30) clamping.Stand on organic glass glue-filling slot 40 then.Unload the organic glass groove behind the encapsulating, with groove 1 under the last groove shift discharge swimming of lotus glue.216,217,226,227 shutoff of jumping a queue respectively of the hole 212,213,222,223 of last groove; The hole of hypomere is the uncork state.Movable embedded electrode device socket plate 23 is inserted the conduit of going up groove.
Claims (7)
1, a kind of bidirectional plate gel electrophoresis device.Contain groove under the electrophoresis (be called for short down groove) and can stand on this time groove certainly, can be inverted groove (groove on the abbreviation) on the electrophoresis of use.Should go up groove and comprise three rectangular panel blocks that equate by width, i.e. two sidewalls, a horizontal stroke section placed in the middle constitutes laterally zygomorphic H shape support.Constitute upward trough floor with this centered rectangle plate, the last groove both sides internal face that is positioned at this base plate upper and lower sides is provided with conduit, and this conduit position is placed in the middle; This conduit can hold movable embedded electrode device socket plate.It is the flat board with character shape that these top and the bottom, two side all are provided with this front face of going up groove of same specification angle circular hole that can add general plug.On this flat board, can stackedly respectively have the recess flat board of different characteristic, thereby constitute the some kinds of different glue unit that carry, reach different and implement.
2, according to the last groove of the described bidirectional plate gel electrophoresis device of the present invention of claim 1.Should go up groove is made of H type support and front and rear wall.Should go up groove and be characterised in that its antetheca is that recess is all offered on the upper and lower limit of a rectangular flat, the upper and lower recess trend of this flat board is and is parallel to each other (the parallel concave-concave flat board of groove abbreviations on, under together).So the feature that should go up groove also one can be inverted use on groove.The vertical edges that should go up the parallel concave-concave flat board of groove is isometric with the sidewall that should go up groove H shape support.The rear wall that should go up groove is a rectangular flat.Its vertical edges is to have constituted one of the condition of being inverted in the dielectrophoresis device of implementing with isometric this feature of sidewall that should go up groove H shape support.
3, can be stacked and placed on the parallel concave-concave flat board of groove according to described of the present invention relates to of claim 1, the recess of this flat board tool feature is a dull and stereotyped identical flat board of parallel concave-concave with last groove.Bottom opens recessed parallel concave-concave feature on this flat board tool.This feature is to have constituted one of the condition of being inverted in the dielectrophoresis device of implementing.
4, establish the recess flat board according to what described of the present invention relates to of claim 1 can be stacked and placed on groove antetheca, tool feature, it is characterized in that the upper end open recess, both sides vertical edges establish fixing between the recessed flat board of list of bar.This feature is to have constituted to implement mobile adhesive tape, thereby replenishes one of condition of encapsulating dielectrophoresis device.
5, according to the described concave-concave mouth flat board that is stacked and placed on the parallel concave-concave flat board of groove that the present invention relates to of claim 1, this concave-concave flat board is characterised in that two recesses are the capwise relation.This feature is to have constituted enforcement original position adhesive tape to turn to one of condition of dielectrophoresis device.
6, establish the recess flat board according to described of being stacked and placed on the parallel concave-concave flat board of groove of the present invention of claim 1, this dull and stereotyped feature is all to establish three recessed flat boards of recess on three limits of rectangular flat.
7, according to the movable embedded electrode device socket plate of the set annex of the last groove of the described bidirectional plate gel electrophoresis device of the present invention of claim 1 and at the conduit of last groove.This feature is to have constituted one of the condition of being inverted in the dielectrophoresis device of implementing.
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CN 02136744 CN1414383A (en) | 2002-09-02 | 2002-09-02 | Bidirectional plate gel electrophoresis device |
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CN 02136744 CN1414383A (en) | 2002-09-02 | 2002-09-02 | Bidirectional plate gel electrophoresis device |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103278549A (en) * | 2004-02-25 | 2013-09-04 | 周伟明 | One-step use method of vertical piece type gel electrophoresis cell |
CN104090018A (en) * | 2014-06-30 | 2014-10-08 | 上海海洋大学 | Standard gel preparation device of nucleic acid mutation detection system and application method thereof |
CN104321126A (en) * | 2012-03-27 | 2015-01-28 | 加利福尼亚大学董事会 | Continuous whole-chip 3-dimensional DEP cell sorter and related fabrication method |
CN110186988A (en) * | 2019-06-18 | 2019-08-30 | 王尧 | A kind of high throughput agarose gel electrophoresis device |
-
2002
- 2002-09-02 CN CN 02136744 patent/CN1414383A/en active Pending
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103278549A (en) * | 2004-02-25 | 2013-09-04 | 周伟明 | One-step use method of vertical piece type gel electrophoresis cell |
CN104321126A (en) * | 2012-03-27 | 2015-01-28 | 加利福尼亚大学董事会 | Continuous whole-chip 3-dimensional DEP cell sorter and related fabrication method |
CN104321126B (en) * | 2012-03-27 | 2017-02-22 | 加利福尼亚大学董事会 | Continuous whole-chip 3-dimensional DEP cell sorter and related fabrication method |
CN104090018A (en) * | 2014-06-30 | 2014-10-08 | 上海海洋大学 | Standard gel preparation device of nucleic acid mutation detection system and application method thereof |
CN110186988A (en) * | 2019-06-18 | 2019-08-30 | 王尧 | A kind of high throughput agarose gel electrophoresis device |
CN110186988B (en) * | 2019-06-18 | 2022-07-05 | 安阳工学院 | High-flux agarose gel electrophoresis device |
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