CN1403584A - Casein enzymolyzing process with compound proteinase to prepare casein phosphopeptide and the use of casein phosphopeptide - Google Patents
Casein enzymolyzing process with compound proteinase to prepare casein phosphopeptide and the use of casein phosphopeptide Download PDFInfo
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- CN1403584A CN1403584A CN01130900A CN01130900A CN1403584A CN 1403584 A CN1403584 A CN 1403584A CN 01130900 A CN01130900 A CN 01130900A CN 01130900 A CN01130900 A CN 01130900A CN 1403584 A CN1403584 A CN 1403584A
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- casein
- compound protease
- cpps
- human body
- phosphopeptide caseinate
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The casein phosphopeptide product (CPPS) has molecular weight below 1000, can promote the absorption of human body to Ca, Fe, Co, Zn, Mn, Se and other beneficial trace elements. It may be used in product for replenishing body's trace elements as well as medicine, functional food and tonic article. The present invention provides one kind of preparation process suitable for industrial production.
Description
The invention provides a kind of easy biotechnology technology, promptly make phosphopeptide caseinate with the compound protease casein hydrolysis.Molecular weight is below 1000.These goods can promote the absorption of human body to beneficial trace elements such as calcium, iron, zinc, manganese, copper, selenium.It is a kind of good metal binding peptide.(CPPs) can form soluble complex to human body beneficial's trace element with calcium, iron etc., solubility calcium, concentration of iron are improved, strengthen the absorption of enteron aisle calcium iron.Because must be in dissolved state during metal ions such as absorption of human body calcium iron on mucous membrane of small intestine could be absorbed by the body effectively, but the PH gradient that exists in the human body, the small intestine environmental PH that particularly is in absorber portion is a meta-alkalescence, make calcium, iron easily be formed insoluble salt, thereby greatly reduce the specific absorption of calcium, iron by phosphoric acid.The present invention separates the CPPs that obtains with compound protease with ox, sheep, mare's milk casease, even also can keep the soluble status of calcium iron under alkaline condition.This is because the absorption of CPPs molecule by the phosphorylation structure from the propylhomoserin residue and calcium, iron.CPPs is the peptide that contains the phosphoserine of cluster, can be used as the carrier of mineral ion, promotes goldbeater's skin to absorption and the utilization to the body healthy trace elements with household such as calcium, iron, zinc, manganese, copper, selenium.
The purpose of this invention is to provide the biological high-tech zymolysis technique of a kind of utilization, ox, sheep, mare's milk casein are resolved into phosphopeptide caseinate and amino acid whose mixture.The preparation method is simple and easy, less investment, and instant effect, purposes is wide, the economic worth height.
Another object of the present invention provides a kind of preparation method that above-mentioned casein hydrolysis makes phosphopeptide caseinate for preparing: get the water that casein adds 1～8 times of weight, reacted 1～50 hour down at 30～90 ℃ with compound protease, stir enzymolysis.Hour carry out in PH1～12 during enzymolysis, after enzymolysis is intact, adopt the inactivation treatment of heating, this solution is through concentrating, spraying drying or lyophilize dry powder.
Phosphopeptide caseinate that enzymolysis ox of the present invention, horse, goat milk casein make and aminoacid mixture molecular weight are below 1000.
Phosphopeptide caseinate that casein hydrolysis of the present invention makes and aminoacid mixture, without any side effects.
Enzymolysis ox of the present invention, sheep, mare's milk casein make phosphopeptide caseinate and aminoacid mixture, are a kind of easy, and lower-cost biological high-tech zymolysis technique production technique is a kind of method of suitable suitability for industrialized production.
The invention provides the purposes of goods of the present invention: be used for medicine, nutritious prod, functional foodstuff, food; Its efficacy effect: promote human body to the absorption to people's healthy trace elements with household such as calcium, iron, zinc, manganese, copper, selenium.
Claims (9)
1, makes phosphopeptide caseinate (CPPs) with the compound protease casein hydrolysis, it is characterized in that a kind of phosphopeptide caseinate (CPPs) and amino acid whose mixture.
2, the phospho-peptide that makes the junket egg with the compound protease casein hydrolysis according to claim 1 includes 20 seed amino acids, altogether in 8 kinds of needed by human and can not synthetic amino acid.
3, goods according to claim 2 is characterized in that molecular weight is below 1000.
4, goods according to claim 3, its feature can promote calcium, iron, zinc, manganese, copper, selenium etc. to human body beneficial's trace elements absorbed, are a kind of good metal binding peptides.
5, goods according to claim 4 is characterized in that the solution of enzymolysis is heated, boils postcooling, solidifies, precipitates, filters.
6, goods according to claim 5 are the powder that concentrated solution, lyophilize or spraying drying are made.
7, the compound protease casein hydrolysis of using according to claim 6 makes phosphopeptide caseinate (CPPs) and aminoacid mixture, and used casein comprises milk, goat milk, mare's milk casein.
8, the used enzyme of phosphopeptide caseinate that makes with the compound protease enzymolysis according to claim 7 is a compound protease.
9, be to be used for medicine, nutritious prod, functional foodstuff, food according to the described junket egg phospho-peptide that makes with the compound protease casein hydrolysis of claim 1～8 and the purposes of aminoacid mixture goods.Its efficacy effect is to promote and strengthen human body calcium, iron, zinc, manganese, copper, selenium etc. are absorbed and utilization human body beneficial's trace element.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN01130900A CN1403584A (en) | 2001-08-31 | 2001-08-31 | Casein enzymolyzing process with compound proteinase to prepare casein phosphopeptide and the use of casein phosphopeptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN01130900A CN1403584A (en) | 2001-08-31 | 2001-08-31 | Casein enzymolyzing process with compound proteinase to prepare casein phosphopeptide and the use of casein phosphopeptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1403584A true CN1403584A (en) | 2003-03-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN01130900A Pending CN1403584A (en) | 2001-08-31 | 2001-08-31 | Casein enzymolyzing process with compound proteinase to prepare casein phosphopeptide and the use of casein phosphopeptide |
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| CN (1) | CN1403584A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1947601B (en) * | 2006-11-10 | 2010-05-12 | 徐佳立 | Application of casein polypeptide phosphate |
| CN101755995B (en) * | 2010-01-08 | 2012-02-08 | 安徽农业大学 | Preparation method of casein phosphopeptide ferrum |
| CN104046673A (en) * | 2013-03-14 | 2014-09-17 | 中国食品发酵工业研究院 | Industrial manufacturing method and use of CPPs-containing hypoallergenic casein peptide whole-powder |
-
2001
- 2001-08-31 CN CN01130900A patent/CN1403584A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1947601B (en) * | 2006-11-10 | 2010-05-12 | 徐佳立 | Application of casein polypeptide phosphate |
| CN101755995B (en) * | 2010-01-08 | 2012-02-08 | 安徽农业大学 | Preparation method of casein phosphopeptide ferrum |
| CN104046673A (en) * | 2013-03-14 | 2014-09-17 | 中国食品发酵工业研究院 | Industrial manufacturing method and use of CPPs-containing hypoallergenic casein peptide whole-powder |
| CN104046673B (en) * | 2013-03-14 | 2020-07-21 | 中国食品发酵工业研究院 | Industrial manufacturing method and application of low-sensitization casein peptide whole powder containing CPPs |
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |