CN1403565A - Fermentation process of producing agaric and its culture medium - Google Patents
Fermentation process of producing agaric and its culture medium Download PDFInfo
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Abstract
The fermentation process of producing agaric includes the steps of: culturing agaric under proper conditions is seed culture medium to obtain fermented agaric seed liquid; inoculating the agaric seed liquid in fermenting tank for further culture under proper conditions in fermented culture medium; and separating agaric mycelium. The present invention also provides culture medium and its usage. The present invention has short fermentation period, high yield, low cost and high agaric polysaccharide content in the product.
Description
Technical field
The present invention relates to the zymotechnique of fungi.More specifically, the present invention relates to fermentation method for producing of umbellate pore furgus and the method for producing polyporusum bellatus, and the substratum that is exclusively used in described method.
Background technology
Umbellate pore furgus (polyporus umbellatus), another name maple Siberian cocklebur, the last of the twelve Earthly Branches Siberian cocklebur, be the polyporaceae fungi, under the native state with the honey mushroom symbiosis.Wild umbellate pore furgus is grown in usage tree root, and spring, Qiu Erji excavate, and remove silt, and drying just can be used.The dry sclerotia of umbellate pore furgus is a kind of Chinese medicine commonly used, since ancient times some regional traditional Chinese medical science of China and working people just umbellate pore furgus in order to the treatment a lump in the abdomen causing distension and pain and tumour, have the medicinal history in more than 2500 year.Cure mainly that acute nephritis, hepatitis, acute urinary tract infection, hot summer weather watery diarrhea, stranguria with turbid discharge, leukorrhea, spleen are wet, four limbs edema (tire is swollen), dysuria etc.
Umbellate pore furgus more and more is subject to people's attention as a kind of Chinese medicine for the treatment of tumour.The water decoction extract of umbellate pore furgus is to treat chronic viral hepatitis and antineoplastic main component at present.The seventies T.Miyazaki and N.oikawa extract polyporusum bellatus first from sclerotia, have immunostimulation through clinical proof, can improve the immunologic function of human body.Polyporusum bellatus is used for the assisting therapy of chemicotherapies such as primary lung cancer, liver cancer, cervical cancer, nasopharyngeal carcinoma, esophagus cancer and leukemia clinically.
Research recently also shows: polyporusum bellatus is a kind of adaptogen (adaptogen), and it can help body to stress adapting to, growth that can also anticancer and kill hiv virus.In addition, it can also impel the main immunocyte CD of control hiv virus
4The breeding growth.At present, polyporusum bellatus has given the dietary supplement sale on the foreign market.
China Academy Of Traditional Chinese Medicine Traditional Chinese Medicine Research Institute has obtained the umbellate pore furgus mycelia in 1979 from the sclerotia separation, among the people's such as Zhu Qin of " bulletin of Chinese materia medica " publication in 1988 the article ＂ umbellate pore furgus ferment filtrate polysaccharide fraction and the mensuration ＂ of mol ratio, with glucose as a standard the sugared peak area of each that records multiply by f/M (M is a molecular weight), the mol ratio of gained filtrate polysaccharide is the D-seminose: the D-semi-lactosi: D-glucose=20: 4: 1, this article is by U.S. CA, MEDLINE, BIOSIS Previews includes (" hunting the mensuration of Siberian cocklebur ferment filtrate polysaccharide fraction and mol ratio ", bulletin of Chinese materia medica 1988 (9) 32～33).
Britain CPI nineteen ninety-five has been introduced by the material of mycelia of extracting umbellate pore furgus or sporophore preparation treatment hepatopathy (" mycelia by extracting umbellate pore furgus or the material of sporophore preparation treatment hepatopathy ", CPI (B) 1995 (9504): 48).From umbellate pore furgus, extract the method for cancer-resisting substance and the antitumor dextran of extraction, also be disclosed in Japanese Patent (patent No. JP55159796) and " the Duction Of Antitumor Glucans By Polyporus Umbellatus Culture " of AMANO PHARMACEUT company limited application in 1979 respectively, Jpn.KokaiTokyo Koho 81 76, in 401,1981.
At present, obtain umbellate pore furgus three kinds of approach arranged:
One, wild excavating.Because therefore wild umbellate pore furgus poor growth excavates difficulty, resource scarcity.
Its two, artificial culture.Umbellate pore furgus is adopted the semi-wild cultivation, because of umbellate pore furgus and honey mushroom battalion cohabitation, so the forest that selects honey mushroom to grow is cultivated.More than two kinds of methods all have the possibilities that heavy metal easily exceeds standard in the umbellate pore furgus bacterium, and output is limited, can not satisfy the demand of growing domestic and international market to umbellate pore furgus far away;
Its three, produce with the biotechnology artificial fermentation.The application ＂ (Chinese Pharmaceutical Journal 1993 (8) 466-468s) of ＂ Armillaria mellea fermented solution in umbellate pore furgus bacterium fermenting process of people such as the Wang Qiu of Inst. of Exploiting of Medical Plant Resource, Chinese Academy of Medical Scienc grain husk report in 1993, substratum is wheat bran-dextrose culture-medium, its composition is wheat bran 50g, glucose 20g, potassium primary phosphate 1.5g, sal epsom 1g, tap water 1000ml, pH6.0～6.5.The processing of fermented liquid: get fermented liquid 10ml to be measured and filter, its filtrate is removed albumen with the Svag method.The proteic liquid to be measured of Ex-all is centrifugal under the condition of 3000rpm, remove starch, check the mensuration that is used for sugar in the supernatant liquor after the complete Ex-all of starch with potassium iodide reagents.The measuring method of sugar: adopt 3.5-dinitrosalicylic acid method.The substratum that contains the fermented liquid of different honey mushroom bacterial strains is cultivated the umbellate pore furgus bacterium in pH5.5～6.0, temperature under 25 ℃ the condition, survey umbellate pore furgus bacterium ball dry weight behind 324h, and bacterium ball dry weight reaches 3.441mg/ml in the fermented liquid, just reaches best up to 324h and produces the sugar phase.The polysaccharide that produces in the treated umbellate pore furgus fermented liquid is separated, purifies, after infrared measurement, this collection of illustrative plates is consistent with the standard polyporusum bellatus collection of illustrative plates of document.
The achievement ＂ that Shandong University identified in 1993 is rich in the fungi series health-care products ＂ of needed by human, utilize solution fermentation to produce large-scale medicine (food) radicula byssoidea and meta-bolitess thereof such as umbellate pore furgus, in fermention medium, on purpose add simultaneously calcium, iron, zinc, selenium, iodine, germanium, human essential elementses such as chromium (trivalent) cobalt, these elements are absorbed in the fungal growth process, change into organic, the radicula byssoidea fermented liquid that obtains in this way contains abundant fungus polysaccharide, plurality of enzymes, multivitamin, reach effective components such as multiple human essential elements, can be at different crowd (old man, child, the pregnant woman, patient) and various disease adopt different ingredients to make oral liquid, tablet, electuary, healthcare products or medicines (" being rich in the fungi series health-care products of human essential elements ", Chinese Appropriate technology achievement 9893701668) such as capsule.
Adding false pseudomonas bacillus, glossy ganoderma, acupuncture needle in the umbellate pore furgus bacterium fermentation culture process of people such as Wang Qiuying 1997 report the fermented liquid of several bacterium such as eats and can make that umbellate pore furgus bacterium nodule number amount increases, weight is big, polysaccharide content is high.Treatment effect the best with false pseudomonas bacillus fermented liquid; When flat board is cultivated, cultivate 25 days colony diameters and reach 72.25mm, in the fermentation culture process, the Crude polysaccharides amount reaches 7.800mg/ml (" fermented liquid of several merits such as false pseudomonas bacillus, glossy ganoderma is to the influence of umbellate pore furgus bacteria growing ", Chinese Pharmaceutical Journal 1997 (8) 459～462) in the best product sugar phase fermented liquid.
The fermentation method for producing of present umbellate pore furgus not only needs to cultivate simultaneously with other bacterium (as honey mushroom), and defective such as the cycle that exists long (10 days), productive rate low (about 0.3%) and/or polyporusum bellatus content is few, therefore, this area presses for the method for the new production umbellate pore furgus of exploitation.
Summary of the invention
Purpose of the present invention just provides the method for a kind of new fermentative production umbellate pore furgus, short, mycelium productive rate height of the cycle of this fermentation process and/or polyporusum bellatus content height.
Another object of the present invention provides a kind of substratum that is exclusively used in this fermentation process.
The inventor is through for many years extensive and deep research, and the key factor of having found the fermentative Production umbellate pore furgus is its medium component, by the repeated screening to each composition of substratum, has developed the new substratum that is used for the umbellate pore furgus fermentation finally.On this basis to the technology of fermentative Production polyporus mycelium and polyporusum bellatus is improved, thereby finished the present invention.
In a first aspect of the present invention, a kind of substratum that is used for the umbellate pore furgus fermentation is provided, by the gross weight of substratum, it contains 0.5-6% bean cake powder, 1～5% carbon source, 0.00005～0.005% VITMAIN B1.
Preferably, described substratum contains the 1-5% bean cake powder; More preferably, described substratum contains the 2-3% bean cake powder.
In a preference, described substratum also contains 0.1-1% yeast powder, 0.3%KH
2PO
4, 0.15%Mg
2SO
4, and described carbon source is 1.5～3.5% glucose.
In second aspect present invention, a kind of method of producing umbellate pore furgus is provided, it comprises step:
(a) under the condition that is fit to the umbellate pore furgus growth, in seed culture medium, cultivate umbellate pore furgus, thereby make the umbellate pore furgus fermentation seed liquid, contain the 0.5-6% bean cake powder in the wherein said seed culture medium, by the weight of substratum;
(b) the umbellate pore furgus fermentation seed liquid that makes in the step (a) is inoculated in fermentor tank, under the condition that is fit to the umbellate pore furgus growth, in fermention medium, the umbellate pore furgus fermentation seed liquid is continued fermentation culture, contain the 0.5-6% bean cake powder in the wherein said fermention medium, by the weight of substratum;
(c) isolate polyporus mycelium.
Preferably, in the method, substratum contains the 1-5% bean cake powder; More preferably, described substratum contains the 2-3% bean cake powder.
In a preference, described substratum also contains 0.1-1% yeast powder, 0.3%KH
2PO
4, 0.15%Mg
2SO
4, and described carbon source is 1.5～3.5% glucose.
In another preference, in step (a) before, also comprise step: with microbiotic umbellate pore furgus is carried out pre-treatment, to kill or to remove assorted bacterium.
In another preference, in step (a), culture temperature is 25 ± 2 ℃, pH5-7.0 (more preferably being 5.5-6.5); In step (b), leavening temperature is 25 ± 2 ℃, pH5.5-7.5 (more preferably being pH5.5-7.0), and air quantity is about 1: 0.3.
In another preference, described method is further comprising the steps of: (d) isolate polyporusum bellatus from polyporus mycelium.More preferably, step (d) comprising: polyporus mycelium is pulverized, and hot-water extraction is filtered, and clear liquid is carried out ultrafiltration, and spraying drying, thereby makes polyporusum bellatus.
In third aspect present invention, a kind of purposes of dregs of beans is provided, it is used to the fermentative production of umbellate pore furgus.
Embodiment
Fermention medium technology, prescription are the key points of umbellate pore furgus bacterium production technology.How to make umbellate pore furgus bacterial classification (polyporus mycelium) under optimum nutritional condition, with the fastest speed breeding growth, the accumulation polyporusum bellatus is a key of the present invention.
As everyone knows, the umbellate pore furgus bacterium is in the symbiosis growth of nature and honey mushroom battalion, and more domestic research units are when research fermentative production umbellate pore furgus, and research emphasis is two bacterium symbiosis condition researchs.The purebred cultivation of umbellate pore furgus bacterium is adopted in this patent invention, selects best Recipe, satisfies the breeding growth needs of umbellate pore furgus bacterium, produces polyporus mycelium to greatest extent, the accumulation polyporusum bellatus.
Umbellate pore furgus decompose utilization better as peptone, but the animal proteinum price is more expensive to animal proteinum.The present invention is through discovering, can use some vegetable-protein also to can be used as the nitrogenous source of umbellate pore furgus bacterium, especially convenient sources, cheap and good-quality dregs of beans (comprising analysis for soybean powder) are particularly suitable as the nitrogenous source of umbellate pore furgus fermentation, and this is the crucial part of umbellate pore furgus fermention medium of the present invention and production technique.
Dregs of beans be beans such as soya bean in producing the soya-bean oil process, oily substance is isolated the formed byproduct in back.The main component of dregs of beans is a protein, contains compositions such as Mierocrystalline cellulose, trace element in addition, not only can provide the umbellate pore furgus fermentation required nitrogenous source, but also can play the effect of inducing the umbellate pore furgus growth.
In umbellate pore furgus fermentation process of the present invention,, as broad as long with the fermentation process of routine except adopting dregs of beans as the nitrogenous source.In the fermentation production process of umbellate pore furgus, can adopt common two step method, promptly cultivate the umbellate pore furgus bacterial classification earlier and obtain seed liquor, then seed liquor is seeded to big fermentor tank, carry out large scale fermentation.In addition, before the umbellate pore furgus bacterial classification is using, should be with carrying out pre-treatment, so that kill or remove the assorted bacterium that may sneak into such as microbiotic such as penicillin, Streptomycin sulphates.
In seed liquor preparation and these two stages (especially in the large scale fermentation stage) of large scale fermentation, all can add dregs of beans as nitrogenous source.Dregs of beans can use separately, also can be used in combination with other nitrogenous sources (as peptone).Dregs of beans can directly be added in culture vessel and the fermentor tank, but preferred mode is to prepare substratum in advance.Soybean meal amount by the gross weight of substratum or fermented liquid, is 0.5-6%, preferably is 1-5%, more preferably is 2-3%.
Behind large scale fermentation, obtain polyporus mycelium according to a conventional method.In addition, also can handle, so that from polyporus mycelium, isolate polyporusum bellatus polyporus mycelium.A kind of method of preferred separation polyporusum bellatus comprises step: polyporus mycelium is pulverized, and hot-water extraction is filtered, and clear liquid is carried out ultrafiltration, and spraying drying, thereby makes polyporusum bellatus.
On the other hand, the present invention also provides a kind of substratum that is used for the umbellate pore furgus fermentation, and by the gross weight of substratum, it contains 0.5-6% bean cake powder, 1～5% carbon source, 0.0005～0.005% VITMAIN B1.
Soybean meal amount by the gross weight of substratum or nutrient solution, is 0.5-6%, preferably is 1-5%, more preferably is 2-3%.
In substratum of the present invention, carbon source is not particularly limited.Its kind and consumption all are conventional.A kind of preferred carbon source is a glucose, and its preferable amount is 1.5-3.5%.
VITMAIN B1 is a kind of material that promotes the umbellate pore furgus growth, and its consumption is generally 0.00005～0.005% or higher, preferably is 0.0005～0.002%, more preferably is 0.0008～0.0015%.
As for other compositions in substratum, for example yeast powder and salt are not particularly limited.Can use corresponding composition and consumption in the conventional umbellate pore furgus substratum always, 0.1-1% yeast powder for example, 0.3%KH
2PO
4, 0.15%Mg
2SO
4And trace element.In addition, in substratum, also can not add these compositions, because contained abundant various materials in the dregs of beans.
The pH value of substratum is generally 5.0-7.5, preferably is 5.5-7.0.
Umbellate pore furgus fermentation process of the present invention and substratum have an outstanding advantage, wherein mainly comprise following advantage:
(1) fermentation period is short
Generally only can finish large scale fermentation in 36 hours, and prior art is generally 10 days.Like this, not only improve the turnover rate of equipment, also significantly lowered production cost, improved output.
(2) productive rate height
Polyporus mycelium is usually greater than 2%, and prior art generally only 2%.
(3) polyporusum bellatus content height
With Fehling method assay method (total reducing sugar-reducing sugar=polysaccharide), polyporusum bellatus content of the present invention is greater than 25%, and polyporusum bellatus content only 0.5% in the wild umbellate pore furgus.
(4) needn't carry out symbiosis culture with other bacterium
In fermenting process of the present invention, needn't cultivate with honey mushroom or other bacterium, be convenient to separate, also be convenient to improve polyporus mycelium purity.
(5) cost is low
Dregs of beans was exactly to make the by product that produces in the oily process originally, and cost of material is far below peptone, thereby had further lowered production cost.
(6) culture medium prescription is simple, and is easy to use
In substratum of the present invention, only need 3 kinds of main components to get final product (being bean cake powder, carbon source and VITMAIN B1), do not need to add other materials usually.In addition, substratum of the present invention has also been realized effective comprehensive utilization of dregs of beans.Usually during the fermentation, needn't regulate pH.
Embodiment further sets forth the present invention below in conjunction with specific embodiment.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example is usually according to the normal condition or the condition of advising according to manufacturer.
Embodiment 1
Orthogonal Method is determined umbellate pore furgus fermentation optimal medium
On the basis of initial medium, change glucose, the concentration of bean cake powder and yeast extract (1-3 sees the following form) is prepared different substratum.With same umbellate pore furgus bacterial classification Polyporus umbellatus (Pers) the Fries umbellate pore furgus 5.173 of equal amts (a ring) (available from Institute of Microorganism, Academia Sinica, Beijing, China) be inoculated in the 500ml triangular flask, in the bottle 75ml nutrient solution is housed, at 25 ℃ ± 1 ℃, the reciprocating type shaking table concussion of 100rpm was cultivated about 7-10 days, measured the biomass of polyporus mycelium then.
Initial medium is the substratum of this area routine, wherein contains following composition:
Component content (%)
Glucose 2.5
Corn steep liquor 1.0
KH
2PO
4????????????????????????0.1
Sal epsom 0.05
Calcium chloride 0.006
Zinc sulfate 0.00004
Manganous sulfate 0.00005
Ferrous sulfate 0.00001
Microorganism B1 0.00005
Table 1 orthogonal test gauge outfit
*Bean cake powder is available from Shanghai Oil Factory No.1.
| ???????A | ??????B | ??????C | |
| Glucose (%) | Bean cake powder *(％) | Yeast extract (%) | |
| ????1 | ?????1.5 | ????1 | ?????0.05 |
| ????2 | ?????2.5 | ????2 | ?????0.1 |
| ????3 | ?????3.5 | ????3 | ?????0.15 |
Table 2 orthogonal experimental design table and result
| ???A | ???B | ???C | Biomass (g/75ml) | |
| ???1 | ???1 | ???1 | ????1 | ????1.2171 |
| ???2 | ???1 | ???2 | ????2 | ????1.6306 |
| ???3 | ???1 | ???3 | ????3 | ????1.9239 |
| ???4 | ???2 | ???1 | ????3 | ????1.0756 |
| ???5 | ???2 | ???2 | ????1 | ????1.4472 |
| ???6 | ???2 | ???3 | ????2 | ????1.9698 |
| ???7 | ???3 | ???1 | ????2 | ????1.3760 |
| ???8 | ???3 | ???2 | ????3 | ????1.6131 |
| ???9 | ???3 | ???3 | ????1 | ????2.1897 |
Table 3 orthogonal experiments analytical table
| ?????A | ?????B | ?????C | |
| ????K1 | ????4.7715 | ????3.6686 | ????4.8539 |
| ????K2 | ????4.4926 | ????4.6908 | ????4.9763 |
| ????K3 | ????5.1787 | ????6.0834 | ????4.6125 |
| ????R | ????0.4072 | ????2.4148 | ????0.3638 |
Orthogonal test analysis is the result show:
(1) best factors combine is A
3B
3C
2, promptly glucose 3.5%, bean cake powder 3.0%, yeast extract 0.1%.
(2) the factor affecting size be B (bean cake powder)＞＞A (glucose)=C (yeast extract), i.e. the effect of bean cake powder is much larger than glucose and yeast extract, and the effect of glucose and yeast extract is close.
Embodiment 2
The preparation of substratum
Press the prescription of table 4, the weighing each component, and mix, behind the adjusting pH, at 1.0kg/cm
2Following High Temperature Sterilization 20 minutes.
Table 4 culture medium prescription
| ????No.1 | ???No.2 | ???No.3 | ???No.4 | ???No.5 | ???No.6 | |
| Glucose | ????2.5 | ????3.0 | ???4.0 | ???1.0 | ???3.0 | ???5.0 |
| Bean cake powder | ????2.0 | ????2.0 | ???1.0 | ???1.0 | ???5.0 | ???0.5 |
| Yeast powder | ????0.2 | ????0.2 | ???1.0 | ???0.2 | ???0.2 | ???0.5 |
| ??KH 2PO 4 | ????0.3 | ????0.3 | ???0.3 | ???0.3 | ???0.3 | ???0.3 |
| ??MgSO 4 | ????0.15 | ????0.15 | ???0.15 | ???0.15 | ???0.15 | ???0.15 |
| VITMAIN B1 | ????0.001 | ????0.001 | ???0.002 | ???0.0001 | ???0.00005 | ???0.0005 |
| Water | Surplus | Surplus | Surplus | Surplus | Surplus | Surplus |
| ??pH | ????5.5 | Uncomfortable pH | Uncomfortable pH | Uncomfortable pH | Uncomfortable pH | Uncomfortable pH |
Embodiment 3
The fermentative production of umbellate pore furgus
(1) preparation of umbellate pore furgus fermentation seed liquid
(A) substratum and bacterial classification
Bacterial classification: umbellate pore furgus bacterial classification Polyporus nmbellatus (Pers) Fries umbellate pore furgus 5.173 (available from Institute of Microorganism, Academia Sinica, Beijing, China)
Slant medium: PDA substratum (potato-glucose agar medium)
Liquid seed culture medium: the No.1 substratum in the table 4.The pH value of this substratum is low to be for the ease of suppressing the growth of assorted bacterium.
(B) seed liquor preparation
Purebred umbellate pore furgus bacterial classification on the inclined-plane is transferred in a plurality of 500ml triangular flasks, the 75ml substratum wherein is housed.Then at 25 ℃ ± 1 ℃, under the 100rpm condition, cultivated about 7-10 days in reciprocating type shaking table concussion, treat the mycelial growth stalwartness, the special light fragrance of umbellate pore furgus bacterium is arranged, when being thick shape, illustrate that seed grows.
In addition, earlier to before inoculation, also available penicillin and/or Streptomycin sulphate microbiotic carry out pre-treatment to the bacterial classification bacterial classification, killing or to remove the assorted bacterium that may sneak into, thereby obtain not purebred umbellate pore furgus bacterial classification with the bacterium of mixing.
(2) large scale fermentation is cultivated
Fermention medium: the No.2 in the table 4, do not regulate pH
Umbellate pore furgus fermentation seed liquid with the rapid middle preparation of previous step is inoculated in 1 ton of fermentor tank by the 5-10% inoculum size.And ferment in following condition, and do not regulate pH during the fermentation:
The jar temperature: 25 ℃ ± 1 ℃,
Air quantity: 1: 0.3,
Rotating speed: 100rpm,
Fermentation period: about 36 hours.
Fermentative production result is as follows: dried mycelia yield is 2.3%, and the cycle is 36 hours.To the infrared check analysis that umbellate pore furgus sporophore and polyporus mycelium carry out, show that the product of large scale fermentation gained conforms to the infared spectrum of contrast umbellate pore furgus, be same species.Through amino acid whose check analysis, show that the aminoacid component of large scale fermentation product is similar to natural umbellate pore furgus with quantity again.
This embodiment shows that zymotechnique of the present invention has possessed big throughput.
Embodiment 4
The preparation of polyporusum bellatus and character check
The polyporus mycelium that makes among the embodiment 3 is ground into particulate below a centimeter, add 17 times of water, under Asia boiling condition, hot-water extraction 2 hours, centrifugal or filtration, clear liquid carries out ultrafiltration with 10,000 molecular weight films, concentrate or 1: 2 volume, 165 ℃ of air intakes, spraying drying under 90 ℃ of conditions of air-out gets polyporusum bellatus.Polyporusum bellatus content is 310mg/ml.
Through Shanghai Normal University microorganism and the check of immunology institute, the polyporusum bellatus molecular weight of acquisition is 29054 dalton.Show that by the immunomodulatory experiment described polyporusum bellatus kills and wounds obviously Lewis lung cancer and HAC liver cancer, has the adjusting immunologic function.
All quote in this application as a reference at all documents that the present invention mentions, just quoted as a reference separately as each piece document.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after having read above-mentioned teachings of the present invention, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Claims (10)
1. a substratum that is used for the umbellate pore furgus fermentation is characterized in that by the gross weight of substratum, it contains 0.5-6% bean cake powder, 1～5% carbon source, 0.00005～0.005% VITMAIN B1.
2. substratum as claimed in claim 1 is characterized in that it contains the 1-5% bean cake powder.
3. substratum as claimed in claim 1 is characterized in that it contains the 2-3% bean cake powder.
4. substratum as claimed in claim 1 is characterized in that it also contains 0.1-1% yeast powder, 0.3%KH
2PO
4, 0.15%Mg
2SO
4, and described carbon source is 1.5～3.5% glucose.
5. method of producing umbellate pore furgus is characterized in that it comprises step:
(a) under the condition that is fit to the umbellate pore furgus growth, in seed culture medium, cultivate umbellate pore furgus, thereby make the umbellate pore furgus fermentation seed liquid, contain the 0.5-6% bean cake powder in the wherein said seed culture medium, by the weight of substratum;
(b) the umbellate pore furgus fermentation seed liquid that makes in the step (a) is inoculated in fermentor tank, under the condition that is fit to the umbellate pore furgus growth, in fermention medium, the umbellate pore furgus fermentation seed liquid is continued fermentation culture, contain the 0.5-6% bean cake powder in the wherein said fermention medium, by the weight of substratum;
(c) isolate polyporus mycelium.
6. method as claimed in claim 5 is characterized in that, in step (a) before, also comprises step: with microbiotic umbellate pore furgus is carried out pre-treatment, to kill or to remove assorted bacterium.
7. method as claimed in claim 5 is characterized in that, in step (a), culture temperature is 25 ± 2 ℃, pH5-7.0; In step (b), leavening temperature is 25 ± 2 ℃, and pH5.5-7.5, air quantity are 1: 0.3.
8. method as claimed in claim 5 is characterized in that, and is further comprising the steps of:
(d) from polyporus mycelium, isolate polyporusum bellatus.
9. method as claimed in claim 8 is characterized in that, step (d) comprising: polyporus mycelium is pulverized, and hot-water extraction is filtered, and clear liquid is carried out ultrafiltration, and spraying drying, thereby makes polyporusum bellatus.
10. the purposes of a dregs of beans is characterized in that, it is used to the fermentative production of umbellate pore furgus.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1332019C (en) * | 2004-05-26 | 2007-08-15 | 张鑫 | Grifola umbellate zymophyte powder and grifola umbellate polysaccharide producing method and products therefrom |
| CN101948760A (en) * | 2010-09-17 | 2011-01-19 | 西北农林科技大学 | Method for producing grifola sterides and polysaccharides by liquid suspension cultivation |
| CN1912100B (en) * | 2005-08-08 | 2011-11-16 | 上海瑞丰农业科技公司 | Fermentation method of polyporus and production method of polyrorus polysaccharide |
| CN103820334A (en) * | 2014-03-13 | 2014-05-28 | 曾化伟 | Method for producing polyporus umbellatus powder through submerged fermentation |
| CN110521490A (en) * | 2016-10-14 | 2019-12-03 | 北京京诚生物科技有限公司 | Lucidum spore powder breeding method |
| CN112840949A (en) * | 2021-01-25 | 2021-05-28 | 中国医学科学院药用植物研究所 | Artificial cultivation method of polyporus umbellatus |
-
2001
- 2001-09-10 CN CNB011267054A patent/CN1173029C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1332019C (en) * | 2004-05-26 | 2007-08-15 | 张鑫 | Grifola umbellate zymophyte powder and grifola umbellate polysaccharide producing method and products therefrom |
| CN1912100B (en) * | 2005-08-08 | 2011-11-16 | 上海瑞丰农业科技公司 | Fermentation method of polyporus and production method of polyrorus polysaccharide |
| CN101948760A (en) * | 2010-09-17 | 2011-01-19 | 西北农林科技大学 | Method for producing grifola sterides and polysaccharides by liquid suspension cultivation |
| CN101948760B (en) * | 2010-09-17 | 2012-05-09 | 西北农林科技大学 | Method for producing grifola sterides and polysaccharides by liquid suspension cultivation |
| CN103820334A (en) * | 2014-03-13 | 2014-05-28 | 曾化伟 | Method for producing polyporus umbellatus powder through submerged fermentation |
| CN110521490A (en) * | 2016-10-14 | 2019-12-03 | 北京京诚生物科技有限公司 | Lucidum spore powder breeding method |
| CN112840949A (en) * | 2021-01-25 | 2021-05-28 | 中国医学科学院药用植物研究所 | Artificial cultivation method of polyporus umbellatus |
| CN112840949B (en) * | 2021-01-25 | 2022-05-27 | 中国医学科学院药用植物研究所 | Artificial cultivation method of polyporus umbellatus |
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| Publication number | Publication date |
|---|---|
| CN1173029C (en) | 2004-10-27 |
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