CN1397156A - Method for culturing natural multi-colour lawn - Google Patents
Method for culturing natural multi-colour lawn Download PDFInfo
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- CN1397156A CN1397156A CN 02136506 CN02136506A CN1397156A CN 1397156 A CN1397156 A CN 1397156A CN 02136506 CN02136506 CN 02136506 CN 02136506 A CN02136506 A CN 02136506A CN 1397156 A CN1397156 A CN 1397156A
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- 238000000034 method Methods 0.000 title claims abstract description 28
- 238000012258 culturing Methods 0.000 title claims abstract description 8
- 231100000350 mutagenesis Toxicity 0.000 claims abstract description 33
- 238000002703 mutagenesis Methods 0.000 claims abstract description 33
- 238000012216 screening Methods 0.000 claims abstract description 22
- 238000009395 breeding Methods 0.000 claims abstract description 19
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims abstract description 10
- 230000001488 breeding effect Effects 0.000 claims abstract description 5
- 241001520823 Zoysia Species 0.000 claims abstract description 4
- 244000025254 Cannabis sativa Species 0.000 claims description 15
- 238000012360 testing method Methods 0.000 claims description 14
- 241000743339 Agrostis Species 0.000 claims description 12
- 238000011156 evaluation Methods 0.000 claims description 12
- 241000196324 Embryophyta Species 0.000 claims description 11
- 230000005855 radiation Effects 0.000 claims description 11
- 238000005286 illumination Methods 0.000 claims description 10
- 230000003505 mutagenic effect Effects 0.000 claims description 10
- 241000234643 Festuca arundinacea Species 0.000 claims description 9
- 210000001161 mammalian embryo Anatomy 0.000 claims description 8
- NNBFNNNWANBMTI-UHFFFAOYSA-M brilliant green Chemical compound OS([O-])(=O)=O.C1=CC(N(CC)CC)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](CC)CC)C=C1 NNBFNNNWANBMTI-UHFFFAOYSA-M 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 239000003471 mutagenic agent Substances 0.000 claims description 6
- 231100000707 mutagenic chemical Toxicity 0.000 claims description 6
- 238000009331 sowing Methods 0.000 claims description 6
- 241001104043 Syringa Species 0.000 claims description 5
- 235000004338 Syringa vulgaris Nutrition 0.000 claims description 5
- 239000002962 chemical mutagen Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 238000009396 hybridization Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 231100000219 mutagenic Toxicity 0.000 claims description 4
- 244000052363 Cynodon dactylon Species 0.000 claims description 3
- 240000004296 Lolium perenne Species 0.000 claims description 3
- 241000044532 Paspalum conjugatum Species 0.000 claims description 3
- 244000292693 Poa annua Species 0.000 claims description 3
- 241000209504 Poaceae Species 0.000 claims description 3
- 241001274961 Rubus repens Species 0.000 claims description 3
- 235000007218 Tripsacum dactyloides Nutrition 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 2
- 241000209140 Triticum Species 0.000 claims description 2
- 235000021307 Triticum Nutrition 0.000 claims description 2
- 230000006698 induction Effects 0.000 claims description 2
- 239000003086 colorant Substances 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 230000001360 synchronised effect Effects 0.000 abstract 1
- 239000007858 starting material Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 108700005079 Recessive Genes Proteins 0.000 description 1
- 102000052708 Recessive Genes Human genes 0.000 description 1
- 230000019552 anatomical structure morphogenesis Effects 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 235000019788 craving Nutrition 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
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- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
A method for culturing the lawn grass with different natural colours includes choosing dried seeds or explant, direct or in-vitro mutagenesis by Co60-gamma ray or sodium azide, multiple screenings and synchronous breeding.
Description
Technical field
The present invention relates to the breeding method of natural multi-colour lawn.
Background technology
The lawn have conserve water and soil, nature remodeling, multiple function such as beautify the environment, play an important role improving the urban human habitat environment and build aspect such as leisure place.Up to now, all can only utilize the single green turfgrass of color in physical culture place and the urban afforestation at home and abroad.In the real life, multiple places (closing) such as football field, golf course, sports ground and celebration have presented craving for multi-colour lawn.Owing to lack the turfgrass of other color, the have to chemical stain grass that adopts chemical dye to tint of some occasion people.
Summary of the invention
The object of the invention is to provide a kind of breeding method of pure natural turfgrass.For achieving the above object, concrete technical measures are such.
The breeding method of natural multi-colour lawn, starting material can adopt the dry seeds or the explant of green turfgrasss such as cold season, warm season or the four seasons are evergreen to carry out.According to the difference of dry seeds or explant two class starting materials, the breeding method of natural multi-colour lawn has two kinds.
Method one: with the dry seeds is the method that material is cultivated multi-colour lawn, it is characterized in that may further comprise the steps:
1) dry seeds of getting cold season, warm season or evergreen turfgrass of the four seasons that moisture content is 13%-13.5% carries out mutagenic treatment as test material with physics or chemical mutagen;
2) live mutagenesis (M in the present age
0) seed, gather in the crops a mutagenesis generation (M by bulk method or individual plant method when ripe
1) seed of plant, be the live mutagenesis of method two generations (M by population mixture method or strain
2) colony, and in two leaf stage with naked eyes screening and separating leaf variegation body;
3) from mutagenesis three generations (M
3) beginning, artificial illumination and temperature culturing room, field natural conditions interval sowing, multiple spot are tested respectively, identify in the strange land and stability observing between year, carrying out the susceptibility such as illumination, temperature, ecology of color leaf trait expression identifies, the multi-colour lawn resource of screening environment non-sensitive type stably express, and to the colored resource of some level grounds with the proterties difference, with it is hybrid strain, by conventional hybridization, backcross and in conjunction with the synchronization breedings such as dwarfing of primitive green kind, by repetition test and the multiplex screening of the above step, get natural multi-colour lawn through several years.
Physical mutagen in the said method is
60Co-gamma-rays, radiation dose are 500-900Gy.Chemical mutagen is sodium azide (NaN
3), treatment dosage is 0.5mMh-3.0mMh.The color that adopts the natural multi-colour lawn that this method obtains is pink, white edge, yellow, yellowish green, emerald green, lilac or their mixing striped.
Method two: with the explant is the method that starting material is cultivated multi-colour lawn, it is characterized in that may further comprise the steps:
1) with explants such as the mature embryo of cold season, warm season or evergreen turfgrass of the four seasons, immature embryo, young stem, young fringes as test material, use the physical mutagen mutagenic treatment;
2) mutagenesis is undertaken by any of following two kinds of methods, and a kind of is that first radiation explant is carried out tissue culture thereafter; Another kind is the first tissue culture callus that forms of radiation explant induction thereafter, after the mutagenesis respectively at a stripped mutagenesis generation (M
1R
1) and stripped mutagenesis two generations (M
2R
2) carry out leaf variegation body screening;
3) stripped certainly mutagenesis three generations (M
3R
3) beginning, respectively by artificial illumination and temperature culturing room, field natural conditions interval sowing, multiple spot test, strange land evaluation and stability observing between year, carrying out the susceptibility such as illumination, temperature, ecology of color leaf trait expression identifies, the multi-colour lawn resource of screening environment non-sensitive type stably express, and to the colored resource of some level grounds with the proterties difference, with it is hybrid strain, by conventional hybridization, backcross and the breeding wheat for semidwarfness of primitive green kind.By repetition test and the multiplex screening of the above step, get natural multi-colour lawn through several years.
Mutagen in this method are
60Co-gamma-rays, the radiation dose of mature embryo are 200-400Gy, and the radiation dose of immature embryo, young stem, young fringe is 1-20Gy.The color that adopts the natural multi-colour lawn that this method obtains is pink, white edge, yellow, yellowish green, emerald green, lilac or their mixing striped.
Said cool-season grasses normally Festuca Arundinacea, English ryegrass, bent grass, annual bluegrass in above-mentioned two kinds of methods, the warm season grass is Bermuda grass, Korea lawn grass, buffalograss normally, four seasons bent grass normally Bai Sanye and dewdrop grass.
The present invention has the advantage of following several respects:
1) the invention serial leaf look and normal green far different natural multi-colour lawn, realized the multiple color of same kind, be the defective of single green thereby filled up world's turfgrass.
2) with the painted grass of pure natural colorful grass instead of chemical, not only free from environmental pollution, and aspect physical culture place, afforestation and advertisement design, as commemorate the preparation of multicolour patterns such as literal, mascot and mark, can realize a series of intention application.
3) because color leaf proterties is controlled by the cell nucleus single recessive gene, colored grass will be in grass seeds be produced the quality and the purity of effective monitoring grass seeds, promptly big Tanaka as long as observe rejecting measure that whether the wounded leaf of careless seedling implement normal green seedling with the seed quality that improves colored grass or add up the prize ratio of Ye Miao and greenery seedling of grass seeds and identify the purity of grass seeds fast, easily.
Description of drawings
Fig. 1 is the mutagenesis and the screening process block diagram of colored Festuca Arundinacea
Fig. 2 is the mutagenesis and the screening process block diagram of colored bent grass
Embodiment
Embodiment 1
Cultivate the pure natural colorful Festuca Arundinacea with the dry seeds mutagenesis:
In September, 1998, get moisture content and be 13.5% Festuca Arundinacea dry seeds and be starting material for 0.5 kilogram, after distilled water seed soaking 16 hours, handle with the chemical mutagen sodium azide, the dosage of handling is that (concentration of sodium azide is 1.0mM to 2.0mMh, processing time is 2 hours, and treatment temperature is 25 ℃).Live mutagenesis M in the present age
0Seed mixes all mutagenesis generation M of receipts with bulk method by the end of May in next year
1The seed of plant.In mid-September, 1999, mixed planting mutagenesis two generations M
2Colony, when growth of seedling during to two leaf stage, screening can significantly have other leaf variegation body with normal green by clear identification leaf look.In May, 2000, press the ripe leaf variegation body seed of individual plant method results.In June, 2000, carry out temperature and photoperiod sensitivity evaluation by artificial illumination and temperature culturing room, reject responsive type, choose the leaf look mutant that keeps non-sensitive type.In September, 2000, respectively three test sites to M
3Non-sensitive type leaf look mutant carries out interval sowing and multiple spot evaluation test under the natural conditions, and screening keeps leaf look mutant.In May calendar year 2001, press leaf look mutant type and mix receipts mutagenesis three generations M
3The seed of plant.The calendar year 2001 6-9 month, get part mutagenesis four generations (M
4) seed carries out leaf look mutant different ecological Adaptability Evaluation in Lingshui, Hainan, keep leaf look mutant.In September calendar year 2001, further carry out M
4Stability, genetic development and morphogenesis research between the year of leaf look mutant.Through above-mentioned multiple evaluation and evaluation, filter out that overall characteristic is similar to the primitive green Festuca Arundinacea, stably express, reach mutagenesis five generations (M from generation to generation
5) colored mutant.Can cultivate pink, lilac, white edge, yellow, yellowish green, emerald green or their pure natural colorful Festuca Arundinaceas such as mixing striped.Concrete colored Festuca Arundinacea brings out sudden change and sees Fig. 1 with the Screening and Identification process.
Embodiment 2
Cultivate the pure natural colorful bent grass with young stem radioinduction:
In July, 1998, from the top of big Tanaka's clip bent grass children's stem (be about 2.0～3.5cm),, be cut into segment (being about 0.5cm), be inoculated in the 1/2MS solid culture medium through surface sterilization, wait to induce more after, give
60The radiation of Co-gamma-rays, the dosage of processing are 5Gy.Irradiation treatment successive transfer culture 1 time after one week goes to and makes it differentiation on the 1/2MS medium that does not contain hormone.After the seedling that differentiates fully grows, go to the greenhouse continued growth, plant division is transplanted to Honda then, isolation implant, results separately.At a stripped mutagenesis generation (M
1R
1) filter out the leaf variegation body.In September, 1999 is from more than 5000 stripped mutagenesis two generations (M
2R
2) in the colony that forms of individual plant, the strain of screening leaf variegation, and press the individual plant method and gather in the crops seed.In June, 2000, carry out temperature and photoperiod sensitivity evaluation by artificial illumination and temperature culturing room, reject responsive type, choose the leaf look mutant that keeps non-sensitive type.In September, 2000, respectively two test sites to exsomatizing mutagenesis three generations (M
3R
3) carry out interval sowing and multiple spot evaluation test under the natural conditions for non-sensitive type leaf look mutant, screening keeps leaf look mutant.In May calendar year 2001, press leaf look mutant type and mix receipts M
3R
3The seed of plant.The calendar year 2001 6-9 month, get the stripped mutagenesis four generations (M of part
4R
4) seed carries out the different ecological Adaptability Evaluation in Lingshui, Hainan, screening keeps leaf look mutant.In September calendar year 2001, further carry out M
4R
4Stability between the year of leaf look mutant.Through above-mentioned multiple evaluation and evaluation, filter out stably express, reach the mutagenesis five generations (M that exsomatizes from generation to generation
5R
5) colored bent grass mutant.Can cultivate white edge, yellow, emerald green or their pure natural colorful bent grass such as mixing striped.Concrete colored bent grass brings out sudden change and sees Fig. 2 with the Screening and Identification process.
Claims (9)
1. the breeding method of natural multi-colour lawn is characterized in that may further comprise the steps:
1) dry seeds of getting cold season, warm season or evergreen turfgrass of the four seasons that moisture content is 13%-13.5% carries out mutagenic treatment as test material with physics or chemical mutagen;
2) live mutagenesis (M in the present age
0) seed, gather in the crops a mutagenesis generation (M by bulk method or individual plant method when ripe
1) seed of plant, be the live mutagenesis of method two generations (M by population mixture method or strain
2) colony, and in two leaf stage with naked eyes screening and separating leaf variegation body;
3) from mutagenesis three generations (M
3) beginning, artificial illumination and temperature culturing room, field natural conditions interval sowing, multiple spot are tested respectively, identify in the strange land and stability observing between year, carrying out the susceptibility such as illumination, temperature, ecology of color leaf trait expression identifies, the multi-colour lawn resource of screening environment non-sensitive type stably express, and to the colored resource of some level grounds with the proterties difference, with it is hybrid strain, by conventional hybridization, backcross and in conjunction with the synchronization breedings such as dwarfing of primitive green kind, by repetition test and the multiplex screening of the above step, get natural multi-colour lawn through several years.
2. by the breeding method of the described natural multi-colour lawn of claim 1, it is characterized in that physical mutagen is
60Co-gamma-rays, the dosage of radiation are 500～900Gy.
3. by the breeding method of the described natural multi-colour lawn of claim 1, it is characterized in that chemical mutagen is a sodium azide, the dosage of processing is 0.5mMh～3.0mMh.
4. press the breeding method of the described natural multi-colour lawn of claim 1, it is characterized in that said cool-season grasses is Festuca Arundinacea, English ryegrass, bent grass, annual bluegrass, the warm season grass is Bermuda grass, Korea lawn grass, buffalograss, the four seasons bent grass be Bai Sanye and dewdrop grass.
5. by the breeding method of the described multi-colour lawn of claim 1, the color that it is characterized in that natural multi-colour lawn is pink, lilac, white edge, yellow, yellowish green, emerald green or their mixing striped.
6. the breeding method of natural multi-colour lawn is characterized in that may further comprise the steps:
1) with explants such as the mature embryo of cold season, warm season or evergreen turfgrass of the four seasons, immature embryo, young stem, young fringes as test material, use the physical mutagen mutagenic treatment;
2) mutagenesis is undertaken by any of following two kinds of methods, and a kind of is that first radiation explant is carried out tissue culture thereafter; Another kind is the first tissue culture callus that forms of radiation explant induction thereafter, after the mutagenesis respectively at a stripped mutagenesis generation (M
1R
1) and stripped mutagenesis two generations (M
2R
2) carry out leaf variegation body screening;
3) stripped certainly mutagenesis three generations (M
3R
3) beginning, respectively by artificial illumination and temperature culturing room, field natural conditions interval sowing, multiple spot test, strange land evaluation and stability observing between year, carrying out the susceptibility such as illumination, temperature, ecology of color leaf trait expression identifies, the multi-colour lawn resource of screening environment non-sensitive type stably express, and to the colored resource of some level grounds with the proterties difference, with it is hybrid strain, by conventional hybridization, backcross and the breeding wheat for semidwarfness of primitive green kind.By repetition test and the multiplex screening of the above step, get multi-colour lawn through several years.
7. by the breeding method of the described natural multi-colour lawn of claim 6, it is characterized in that mutagen are
60Co-gamma-rays, the radiation dose of mature embryo are 200-400Gy, and the radiation dose of immature embryo, young stem, young fringe is 1-20Gy;
8. press the breeding method of the described natural multi-colour lawn of claim 6, it is characterized in that said cool-season grasses is Festuca Arundinacea, English ryegrass, bent grass, annual bluegrass, the warm season grass is Bermuda grass, Korea lawn grass, buffalograss, the four seasons bent grass be Bai Sanye and dewdrop grass.
9. by the breeding method of the described natural multi-colour lawn of claim 6, the color that it is characterized in that multi-colour lawn is pink, lilac, white edge, yellow, yellowish green, emerald green or their mixing striped.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021365067A CN1188028C (en) | 2002-08-12 | 2002-08-12 | Method for culturing natural multi-colour lawn |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021365067A CN1188028C (en) | 2002-08-12 | 2002-08-12 | Method for culturing natural multi-colour lawn |
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| Publication Number | Publication Date |
|---|---|
| CN1397156A true CN1397156A (en) | 2003-02-19 |
| CN1188028C CN1188028C (en) | 2005-02-09 |
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ID=4748670
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|---|---|---|---|
| CNB021365067A Expired - Fee Related CN1188028C (en) | 2002-08-12 | 2002-08-12 | Method for culturing natural multi-colour lawn |
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| CN (1) | CN1188028C (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101889505A (en) * | 2010-06-22 | 2010-11-24 | 江西天顺生态农业有限公司 | Radiation breeding method for gardenia |
| CN103229642A (en) * | 2013-02-20 | 2013-08-07 | 张永清 | Breeding method for improved variety of Lonicera japonica Thunb No.1 |
| CN103733993A (en) * | 2013-12-17 | 2014-04-23 | 中国农业大学 | Inducing method of Buchloe dactyloides callus |
| CN104429926A (en) * | 2014-11-07 | 2015-03-25 | 江苏省中国科学院植物研究所 | Interspecific hybridization breeding method for new zoysia plant variety |
| CN110663544A (en) * | 2019-11-04 | 2020-01-10 | 北京市农林科学院 | Carex viridis seed radiation mutation breeding method |
| CN112715355A (en) * | 2020-12-29 | 2021-04-30 | 河南省锄禾园林草业服务有限公司 | Safe and efficient mutation method for bluegrass |
| CN114303814A (en) * | 2021-12-29 | 2022-04-12 | 北京市农林科学院 | Radiation mutation breeding method for 'purple leaf' pennisetum |
-
2002
- 2002-08-12 CN CNB021365067A patent/CN1188028C/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101889505A (en) * | 2010-06-22 | 2010-11-24 | 江西天顺生态农业有限公司 | Radiation breeding method for gardenia |
| CN101889505B (en) * | 2010-06-22 | 2012-02-15 | 江西天顺生态农业有限公司 | Radiation breeding method for gardenia |
| CN103229642A (en) * | 2013-02-20 | 2013-08-07 | 张永清 | Breeding method for improved variety of Lonicera japonica Thunb No.1 |
| CN103733993A (en) * | 2013-12-17 | 2014-04-23 | 中国农业大学 | Inducing method of Buchloe dactyloides callus |
| CN103733993B (en) * | 2013-12-17 | 2015-08-05 | 中国农业大学 | A kind of abductive approach of buffalograss callus |
| CN104429926A (en) * | 2014-11-07 | 2015-03-25 | 江苏省中国科学院植物研究所 | Interspecific hybridization breeding method for new zoysia plant variety |
| CN110663544A (en) * | 2019-11-04 | 2020-01-10 | 北京市农林科学院 | Carex viridis seed radiation mutation breeding method |
| CN110663544B (en) * | 2019-11-04 | 2021-04-20 | 北京市农林科学院 | Carex viridis seed radiation mutation breeding method |
| CN112715355A (en) * | 2020-12-29 | 2021-04-30 | 河南省锄禾园林草业服务有限公司 | Safe and efficient mutation method for bluegrass |
| CN114303814A (en) * | 2021-12-29 | 2022-04-12 | 北京市农林科学院 | Radiation mutation breeding method for 'purple leaf' pennisetum |
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|---|---|
| CN1188028C (en) | 2005-02-09 |
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