CN1387922A - Quick-hemagglutination hemostasis sponge - Google Patents
Quick-hemagglutination hemostasis sponge Download PDFInfo
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- CN1387922A CN1387922A CN 02109638 CN02109638A CN1387922A CN 1387922 A CN1387922 A CN 1387922A CN 02109638 CN02109638 CN 02109638 CN 02109638 A CN02109638 A CN 02109638A CN 1387922 A CN1387922 A CN 1387922A
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- chitosan
- collagen protein
- hemagglutination
- hemostasis
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Abstract
The quick-hemagglutination hemostasis sponge is one absorbable hemostasis material used in surgical operation. It contains collagen and chitosan in the weight ratio of 1-4 to 6-12. It is spongy solid prepared through dissolving collagen and chitosan separately in glacial acetic acid, mixing and vacuum freeze drying. It has the advantages of strong hemostasis function capable of arresting bleeding even in the case of reduced thrombocyte; antibiosis performance owing to the bacteriostasis of chitosan; excellent biocompatibility without toxic side effect; and good toughness without breaking in blood.
Description
Technical field: the present invention relates in a kind of surgical operation the absorbable hemostatic material that uses at tissue surface.
Background technology: in surgical operation, hemostatic material plays an important role.In the last few years collagen protein be studies show that collagen protein was best in naturalness lower hemostasia effect.Collagen hemostasis agent hemostasis efficient height, few side effects is a kind of ideal hemostatic material.Find that in the research aspect chitosan chitin has and sticks and the pain relieving effect its derivant chitosan, as N, O-carboxymethyl chitosan and chitosan acetas have hemostasia effect, and chitosan produces haemostatic effect with the less erythrocyte effect that comes into the picture in coagulation process.The hemostasis of chitosan, antibacterial, antibiotic property, biocompatibility, promotion organization healing and the character that is easy to form gel have all been given it and have been used for the superperformance of bleeding-stopping dressing or hemorrhage.Domestic preparation about collagen haemostatic sponge at present is less.Domestic absorbable gelatin sponge commonly used only under platelet and the normally functioning situation of thrombin, just can finish hemostatic function, and its anti-microbial property is poor at present; The toughness of gelfoam is also relatively poor, and is after especially soaking in blood, broken easily.
Summary of the invention: the present invention develops a kind of quick-hemagglutination hemostasis sponge, and what this sthptic sponge had solved that gelfoam exists only just can finish hemostatic function under platelet and thrombin function normal condition, with and anti-microbial property is poor, toughness is relatively poor problem.Oxazepan sthptic sponge of the present invention comprises collagen protein and two kinds of compositions of chitosan, the weight ratio of chitosan and collagen protein is (1～4): (6～12), above-mentioned oxazepan sthptic sponge is to be dissolved in respectively in the glacial acetic acid solution by chitosan and collagen protein, then through the spongiform solid that mixes, lyophilization is made.Used chitosan is N, O-carboxymethyl chitosan or chitosan acetas.The degree of deacetylation of above-mentioned chitosan is 40%～85%.Above-mentioned collagen protein is the collagen protein of cattle heel string or the preparation of pig heel string enzymatic isolation method, or the collagen protein of Corii Bovis seu Bubali enzymatic isolation method preparation, and the optimum weight ratio of above-mentioned chitosan and collagen protein is (3～4): (7～8).The manufacture method of oxazepan sthptic sponge of the present invention is: 1, by chitosan: collagen protein==(1～4): the weight ratio of (6～12) is measured two kinds of raw materials respectively; 2, be that to be mixed with concentration be 0.5%～3% solution for 0.5%～5% glacial acetic acid with chitosan concentration; 3, be that to be mixed with concentration be 1%～4% solution for 0.5%～3% glacial acetic acid with collagen protein concentration; 4, chitosan and two kinds of solution of collagen protein are mixed, then mixed liquor is promptly obtained the oxazepan sthptic sponge through vacuum lyophilization.The comparative study result such as the following table of this oxazepan sthptic sponge and gelfoam are listed:
Rabbit ear portion vein, liver, spleen haemostatic effect contrast table:
| Hemostatic material | The example number | Average bleeding stopping period (S) | Hemostasis percentage rate (%) | ||
| Ear | Liver | Spleen | |||
| Quick-hemagglutination hemostasis sponge | 20 | ????55±8 | ??58±6 | ????62±10 | ????100 |
| Gelfoam | 20 | ????63±11 | ??704±15 | ????65±8 | ????100 |
Through discovering of comparative, this quick-hemagglutination hemostasis sponge all is being better than gelfoam aspect the bio-physical property of haemostatic effect and hemostatic material.This quick-hemagglutination hemostasis sponge has following advantage: the one, and powerful hemostatic function, it can be issued to the hemostasis purpose in the thrombocytopenia situation; The 2nd, it has anti-microbial property, and chitosan can bacteria growing inhibiting, so this quick-hemagglutination hemostasis sponge also has anti-microbial property; The 3rd, it has excellent biological compatibility, absorbs well in vivo, does not have toxic and side effects; The 4th, its toughness is good, soaks the back and be difficult for broken in blood.
The specific embodiment one: the quick-hemagglutination hemostasis sponge of present embodiment is that 0.5%～5% glacial acetic acid solution is made by chitosan, collagen protein and concentration.The weight ratio of chitosan and collagen protein is (1～3): (6～9).Its manufacture method is: 1, take by weighing chitosan and collagen protein by above-mentioned weight ratio; 2, chitosan being mixed with concentration with glacial acetic acid solution is 0.5%～3% solution; 3, collagen protein being mixed with concentration with glacial acetic acid solution is 1%～4% solution; 4, above-mentioned two kinds of solution are mixed, then mixed liquor is promptly obtained quick-hemagglutination hemostasis sponge through vacuum lyophilization.The extraction process of above-mentioned collagen protein is: 1, that the cattle heel string is extremely translucent with the 5%NaOH solution soaking.2, discard fatty tissue, the heel string of hardening is cut into the fritter of the about 4mm of diameter; 3, with organizing pulverizer that heel string is twisted into pasty state at a high speed; 4, the centrifugal moisture content of removing, 5, use Na
2HPO
4(0.1M), water respectively gives a baby a bath on the third day after its birth time, leaves and takes precipitate, 6, enzymolysis, it is in 2 the hydrochloric acid solution that pepsin is dissolved in pH value, and precipitate is immersed in the pepsin solution, treats that solution becomes the transparent adhesive tape shape, show that enzymolysis finishes: 7, stop enzymolysis, reconcile making PH 〉=6 with 20%NoOH; 8, saltout, it is in 2 the hydrochloric acid solution that zymolyte is dissolved in pH value, adds 8%NaCl and produces white precipitate; 9, the centrifugal part of anhydrating is dissolved precipitate with 2%HCl, repeats to saltout; 10,, get white powder with the precipitate lyophilization.The preparation technology of above-mentioned chitosan is: 1, the 25g carapace is placed 20%NaOH (300ml) solution to be heated to 110 ℃, return and heat up in a steamer 4hr; 2, the lixiviating liquid that inclines, and washing; 3, add 50%NaOH, 1%NaBH
4Solution quickly heats up to 145 ℃, returns and heats up in a steamer 1hr; 4, the lixiviating liquid that inclines, tap water flushing is to neutral, and the reuse distilled water is given a baby a bath on the third day after its birth time; 5, in 80 ℃ of baking ovens, dry, can get colourless chitosan product; 6, products obtained therefrom is dissolved in 2% acetum, is neutralized to alkalescence with 20%NaOH then, the adularescent flocculent deposit generates, and precipitation separation is washed to neutrality, and product is got final product in 80 ℃ of oven dry.
The specific embodiment two: what present embodiment and the specific embodiment one were different is: the weight ratio of chitosan and collagen protein is (1～3): (9～12).Other composition and manufacture method are identical with the specific embodiment one.
The specific embodiment three: what present embodiment and the specific embodiment one were different is: the weight ratio of chitosan and collagen protein is (3～4): (6～9).Other composition and manufacture method are identical with the specific embodiment one.
The specific embodiment four: what present embodiment and the specific embodiment one were different is: the weight ratio of chitosan and collagen protein is (3～4): (9～12).Other composition and manufacture method are identical with the specific embodiment one.
Claims (4)
1, quick-hemagglutination hemostasis sponge, it is characterized in that it comprises collagen protein and two kinds of compositions of chitosan, the weight ratio of chitosan and collagen protein is (1～4): (6～12), it is to be dissolved in respectively in the glacial acetic acid solution by chitosan and collagen protein, then through the spongiform solid that mixes, vacuum lyophilization is made.
2, quick-hemagglutination hemostasis sponge according to claim 1 is characterized in that used chitosan is N, O-carboxymethyl chitosan or chitosan acetas.
3, according to claim 1,2 described quick-hemagglutination hemostasis sponges, the degree of deacetylation that it is characterized in that above-mentioned chitosan is 40%～85%.
4, quick-hemagglutination hemostasis sponge according to claim 1 is characterized in that above-mentioned collagen protein is the collagen protein of cattle heel string or the preparation of pig heel string enzymatic isolation method, or the collagen protein of Corii Bovis seu Bubali enzymatic isolation method preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02109638 CN1387922A (en) | 2002-04-30 | 2002-04-30 | Quick-hemagglutination hemostasis sponge |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02109638 CN1387922A (en) | 2002-04-30 | 2002-04-30 | Quick-hemagglutination hemostasis sponge |
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| CN1387922A true CN1387922A (en) | 2003-01-01 |
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| CN 02109638 Pending CN1387922A (en) | 2002-04-30 | 2002-04-30 | Quick-hemagglutination hemostasis sponge |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1321704C (en) * | 2004-03-24 | 2007-06-20 | 华东理工大学 | Method for fabricating activated artificial skin tissue in bilayer by using bioreactor |
| CN100415304C (en) * | 2006-03-13 | 2008-09-03 | 西北大学 | Biological degradable hemostatic sponge material and its preparing method |
| CN101816802A (en) * | 2010-02-05 | 2010-09-01 | 佘振定 | Chitosan-based medical dressing |
| CN102049061A (en) * | 2010-12-08 | 2011-05-11 | 苏州同科生物材料有限公司 | Method for preparing chitosan collagen sponge burn dressing |
| CN103848926A (en) * | 2012-11-30 | 2014-06-11 | 江春平 | Preparation method and applications of carboxylation chitosan |
| CN106139238A (en) * | 2016-07-07 | 2016-11-23 | 浙江大学 | A kind of chitosan collagen sponge dressing and preparation method thereof |
| CN109303930A (en) * | 2018-09-27 | 2019-02-05 | 湖南然元医用高科技蛋白线有限公司 | A kind of Medical absorbable antiseptic dressing and preparation method thereof |
| CN109701065A (en) * | 2019-01-03 | 2019-05-03 | 中国人民解放军总医院 | A kind of preparation process for the chitin biodressing being coated with small molecule lithium chloride |
| CN110368518A (en) * | 2019-03-19 | 2019-10-25 | 易杨华 | Rubidium salt styptic sponge and its application |
| CN110585139A (en) * | 2019-10-12 | 2019-12-20 | 青岛世泽生物技术有限公司 | Preparation process of bacteriostatic balanced adsorption dry and comfortable velvet for women |
| CN113995882A (en) * | 2021-12-13 | 2022-02-01 | 山东鑫珂海洋生物技术股份有限公司 | Preparation method of chitin medical dressing for rapid hemostasis |
| CN114350009A (en) * | 2021-10-29 | 2022-04-15 | 北京健康广济生物技术有限公司 | Hemostatic sponge and preparation method thereof |
-
2002
- 2002-04-30 CN CN 02109638 patent/CN1387922A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1321704C (en) * | 2004-03-24 | 2007-06-20 | 华东理工大学 | Method for fabricating activated artificial skin tissue in bilayer by using bioreactor |
| CN100415304C (en) * | 2006-03-13 | 2008-09-03 | 西北大学 | Biological degradable hemostatic sponge material and its preparing method |
| CN101816802A (en) * | 2010-02-05 | 2010-09-01 | 佘振定 | Chitosan-based medical dressing |
| CN102049061A (en) * | 2010-12-08 | 2011-05-11 | 苏州同科生物材料有限公司 | Method for preparing chitosan collagen sponge burn dressing |
| CN103848926A (en) * | 2012-11-30 | 2014-06-11 | 江春平 | Preparation method and applications of carboxylation chitosan |
| CN103848926B (en) * | 2012-11-30 | 2016-03-02 | 江春平 | A kind of preparation method of using carboxyl chitosan and purposes |
| CN106139238A (en) * | 2016-07-07 | 2016-11-23 | 浙江大学 | A kind of chitosan collagen sponge dressing and preparation method thereof |
| CN109303930A (en) * | 2018-09-27 | 2019-02-05 | 湖南然元医用高科技蛋白线有限公司 | A kind of Medical absorbable antiseptic dressing and preparation method thereof |
| CN109701065A (en) * | 2019-01-03 | 2019-05-03 | 中国人民解放军总医院 | A kind of preparation process for the chitin biodressing being coated with small molecule lithium chloride |
| CN109701065B (en) * | 2019-01-03 | 2020-07-17 | 中国人民解放军总医院 | Preparation process of chitosan biological dressing coated with micromolecular lithium chloride |
| CN110368518A (en) * | 2019-03-19 | 2019-10-25 | 易杨华 | Rubidium salt styptic sponge and its application |
| CN110585139A (en) * | 2019-10-12 | 2019-12-20 | 青岛世泽生物技术有限公司 | Preparation process of bacteriostatic balanced adsorption dry and comfortable velvet for women |
| CN114350009A (en) * | 2021-10-29 | 2022-04-15 | 北京健康广济生物技术有限公司 | Hemostatic sponge and preparation method thereof |
| CN113995882A (en) * | 2021-12-13 | 2022-02-01 | 山东鑫珂海洋生物技术股份有限公司 | Preparation method of chitin medical dressing for rapid hemostasis |
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Owner name: YANG BAOFENG Free format text: FORMER OWNER: NONE Effective date: 20030703 |
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Effective date of registration: 20030703 Address after: The Post Street Nangang District 150001 Heilongjiang city of Harbin Province, Harbin Medical University No. 23 hospital neurosurgery Applicant after: Yue Wu Co-applicant after: Yang Baofeng Address before: The Post Street Nangang District 150001 Heilongjiang city of Harbin Province, Harbin Medical University No. 23 hospital neurosurgery Applicant before: Yue Wu |
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