CN1387868A - Prepn process and new use of general asiaticoside - Google Patents
Prepn process and new use of general asiaticoside Download PDFInfo
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- CN1387868A CN1387868A CN 01113014 CN01113014A CN1387868A CN 1387868 A CN1387868 A CN 1387868A CN 01113014 CN01113014 CN 01113014 CN 01113014 A CN01113014 A CN 01113014A CN 1387868 A CN1387868 A CN 1387868A
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Abstract
The present invention relates to medicinal technology field and is the preparation of total asiaticoside and its application for treating depression. Its preparation includes three steps of extraction, purification and crystallization. During the improved purification, the total asiaticoside extraction liquid is absorbed with macroporous resin, depurated with eluting distillated water and eluted with water containing alcohol. The purification process is simple and has less environmental pollution caused by organic solvent. The total asiaticoside may become one kind of natural medicine for treating depression.
Description
The present invention relates to medical technical field, is a kind of new method of preparation Total Of Triterpenes and the new purposes of treatment depression thereof.
Depression be with remarkable and persistent depressed, behavior is numb and pessimistic and worldweary be one group of disease of principal character.In fact it is very common, so that be called as " common cold " in the mental sickness, it still is serious, life-threatening disease simultaneously, is tormenting the people who counts in the world in necessarily.The medicine that is used for the treatment of depression at present mainly contains tricyclic antidepressants, oxidase inhibitor and 5-hydroxy tryptamine cell reabsorption inhibitor, but side effect is in various degree arranged all, as drowsiness, blurred vision, hypertension, convulsions and hyposexuality etc., be extensive use of thereby limited it.
Herba Centellae Centella asiatica (L.) Urban is a samphire, and wide geographic area all has distribution on the south China the Changjiang river, and its extract Total Of Triterpenes has the effect that promotes wound healing, reduces the keloid generation, and effect is remarkable.Medicine for external use (as the accumulated snow frost) and oral medicine (as the accumulated snow sheet) Total Of Triterpenes have been prepared into now, but this medicine only limits to treat wound, do not see the report that Total Of Triterpenes is used for the treatment of depression up to now as yet, finding that through experiment Total Of Triterpenes has antidepressant effect preferably, is a kind of more rising natural antidepressant.The preparation method step complexity of the Total Of Triterpenes of having reported, with an organic solvent kind and quantity are more, easily cause environmental pollution.
One of purpose of the present invention is to provide that a kind of technology is simple, yield is high, is difficult for causing the Total Of Triterpenes novel preparation method of environmental pollution.
Another purpose of the present invention is to provide a kind of new purposes for the treatment of depression to Total Of Triterpenes.
The preparation method of Total Of Triterpenes comprises extraction, purification, crystallization three steps.Extraction of the present invention is identical with former technology with crystallization process, but the purge process difference.Former purifying process adopts halogenating agent (chloroform, dichloromethane etc.) extraction Herba Centellae hydrous alcohol extraction thing, abandon the halogenating agent layer, with Total Of Triterpenes in higher alcohol (n-butyl alcohol, the sec-butyl alcohol etc.) aqueous layer extracted, with sodium hydrate aqueous solution and solution washing higher alcohol layer extract, and with its concentrating under reduced pressure, its shortcoming is the method complexity, process is loaded down with trivial details, use more organic solvent such as halogenating agent, higher alcohol, environmental pollution is serious, also is harmful to healthy (preparation method sees patent CN for details 1194154A number) of actual operator.The present invention then with the Total Of Triterpenes extracting solution through Saponin absorbent-type macroporous resin adsorption, behind distilled water eluting removal impurity,, collect eluent and also be evaporated to dried with aqueous alcohol eluting Total Of Triterpenes, obtain thick thing, reuse aqueous alcohol dissolving back is with regard to the in addition crystallization of available ethyl acetate.This process steps is simple, and kind and quantity that organic solvent uses obviously reduce, thereby have both shortened technological process, has reduced again to the pollution of environment with to the harm of operator's health.Preparation method concrete steps of the present invention are as follows: one, the extraction 1 of Total Of Triterpenes, soak Herba Centellae with aqueous alcohol, obtain the Total Of Triterpenes extracting solution; 2, freezing extracting solution, filtration under diminished pressure is removed precipitate, gets filtrate; Two, the purification 1 of Total Of Triterpenes, according to a conventional method with said extracted liquid concentrating under reduced pressure, obtain concentrated solution; 2, the filtration under diminished pressure concentrated solution obtains filtrate; 3, filtrate is through Saponin absorbent-type macroporous resin adsorption, and the distilled water eluting is removed impurity, again to contain the methanol or the ethanol water eluting of alcohol amount 5%～95%, obtain eluent, (Qi Chen edits, and the People's Health Publisher publishes, 1993:70) to see " herbal pharmacology research methodology " for details; 4, according to a conventional method eluent is evaporated to driedly, obtains thick thing; 5, dissolve thick thing with aqueous alcohol, ratio is: thick thing weight (kilogram): the volume of aqueous alcohol (milliliter)=1: 1～200 obtain Total Of Triterpenes solution.Three, the crystallization 1 of Total Of Triterpenes, with above-mentioned Total Of Triterpenes solution with the ethyl acetate crystallization, ratio is: the volume of Total Of Triterpenes solution (milliliter): the volume of ethyl acetate (milliliter)=1: 1～200, be evaporated to driedly according to a conventional method, obtain settled powder grass general glycoside.
Being used for aqueous alcohol solutions of the present invention, to contain alcohol amount be 5%～95% methanol or ethanol.Warp
13C and
1H magnetic resonance detection, testing result illustrate that the asiatic centella triterpenoid total saponins composition that obtains mainly is made up of Asiaticoside and madecassoside as shown in Table 1 and Table 2, and two kinds of compositions account for more than 60% of this extract.Asiaticoside and madecassoside in the table 1 extract sample
13C chemical shift of NMR value and standard substance are relatively
(being dissolved in pyridine) carbon Herba Centellae Asiaticoside hydroxyl accumulated snow hydroxyl Herba Centellae carbon Herba Centellae Asiaticoside hydroxyl accumulated snow hydroxyl Herba Centellae
1 48.1 48.2 49.9 49.9 27 23.4 23.7 23.7 23.82 68.6 68.9 68.8 69.0 28 176.0 176.2 176.0 179.83 78.4 78.7 78.3 78.5 29 17.1 17.3 23.5 24.04 43.4 43.1 42.9 43.1 30 21.0 21.1 21.0 21.35 47.9 48.7 48.2 48.6 6 19.1 19.3 67.3 67.6 1 95.3 95.5 95.5 95.57 32.9 33.0 39.1 39.0 2 75.0 73.6 75.1 73.68 39.9 39.5 39.4 39.5 3 77.6 78.3 77.6 78.39 48.1 48.3 48.3 48.9 4 70.0 70.1 70.0 70.410 38.0 37.9 37.8 38.0 5 76.2 76.6 76.2 76.711 24.3 24.3 24.5 25.0 6 69.1 71.0 70.7 71.012 125.7 126.0 126.1 126.3 ’13 138.2 137.7 137.5 138.6 1 104.6 104.4 104.6 104.014 42.3 44.2 42.3 44.3 2 73.5 75.0 73.7 75.015 28.4 28.7 28.5 28.7 3 78.4 77.6 78.3 77.616 24.3 24.5 24.5 26.0 4 77.7 78.3 78.0 78.317 49.5 48.4 48.4 48.0 5 76.9 76.3 76.8 76.318 52.9 53.3 53.1 53.3 6 61.0 61.4 61.0 61.619 38.8 38.0 37.9 38.1 20 39.0 39.0 38.8 39.1 1 102.4 102.4 102.4 102.421 30.5 31.0 30.5 31.0 2 72.5 72.4 72.5 72.422 36.5 37.5 36.6 37.5 3 72.3 72.1 72.3 72.123 66.2 66.5 63.7 66.6 4 73.7 73.6 73.5 73.624 14.1 15.5 15.8 15.5 5 70.7 69.4 69.1 69.425 17.9 17.5 17.1 17.2 6 17.4 18.1 18.3 18.126 18.3 18.5 19.0 18.62 1H chemical shift of NMR value and standard substance are relatively
(being dissolved in pyridine)
Hydrogen Herba Centellae Asiaticoside hydrogen hydroxyl accumulated snow hydroxyl Herba Centellae
The careless glycoside sample of glycoside sample standard product glycoside standard substance
2 3.70ddd 3.67ddd 2 3.70ddd 3.71ddd
3 3.36d 3.34d 3 3.36d 3.38d
12 5.24t 5.24t 12 5.24t 5.23t
18 2.20d 2.24d 18 2.20d 2.23d
19 1.36m 1.38m 19 1.36m 1.36m
23 3.28d; 3.51d 3.46d, 3.26d 23 3.37d 3.39d methyl-24 0.69s 0.71s methyl-24 0.73s 0.77s methyl-25 1.04s 1.04s methyl-25 1.04s 1.07s methyl-26 0.84s 0.82s methyl-26 0.81s 0.82s methyl-27 1.13s 1.13s methyl-27 1.10s 1.10s methyl-29 0.92s 0.91d methyl-29 0.92s 0.92s methyl-30 0.96s 1.00s methyl-30 0 95s 0.95s glucose glucose
1 5.29d 5.29d 1 5.29d 5.29d
2 3.33m 3.31m 2 3.31m 3.31m
3 3.37m 3.39m 3 3.39m 3.39m
4 3.42m 3.42m 4 3.40m 3.42m
5 3.48m 3.49m 5 3.47m 3.49m
6a 4.06dd 4.06dd 6a 4.06dd 4.06dd
6b 3.76dd 3.77dd 6b 3.76dd 3.77dd glucose ' glucose '
1 4.37d 4.37d 1 4.36d 4.37d
2 3.23t 3.23t 2 3.23t 3.23t
3 3.43t 3.45t 3 3.44t 3.45t
4 3.51t 3.53t 4 3.51t 3.53t
5 3.29m 3.29m 5 3.29m 3.29m
6a 3.81d 3.80d 6a 3.80d 3.80d
6b 3.65dd 3.65dd 6b 3.65dd 3.65dd rhamnose rhamnose
1 4.83d 4.84d 1 4.84d 4.84d
2 3.83dd 3.83dd 2 3.81dd 3.83dd
3 3.60dd 3.62dd 3 3.62dd 3.62dd
4 3.39t 3.40t 4 3.41t 3.40t
5 3.96d 3.96d, 5 3.97d 3.96d methyl 1.26d 1.27d methyl 1.27d 1.27d four, Total Of Triterpenes are to the checking of experimental depression therapeutical effect
Forced swimming experiment (forced swimming test) and the long-term stress stimulation experiment (chronicunpredictable stress test) of precognition be that the checking medicine has antidepressant effect zoopery commonly used, so verify the preventive and therapeutic effect of Total Of Triterpenes to the experimental animal models depression with it.1, laboratory animal
The SD rat, male, 160～200 grams, per 4/cage group support is freely looked for food and is drunk water 23 ± 2 ℃ of room temperatures, natural lighting.2, experimental technique (1) forced swimming experiment: rat is put into 15 centimetres of the depth of waters, and swimming in the graduated cylinder that water temperature is 25 ℃ (height is 40 centimetres, and diameter is 18 centimetres) makes it produce the feared state of mind, takes out after 15 minutes, does hairy down for 32 ℃.In 24 hours, to laboratory animal injection or gastric infusion, again rat is put into above-mentioned environment respectively, measure rat and in 5 minutes, keeps the motionless time of swimming, with the length of dead time standard as judgement medicine antidepressant effect.Method sees " modern pharmacology experimental technique " (Zhang Juntian chief editor, combined publication society of China Concord Medical Science University of Beijing Medical University publishes, first volume 1998:1064) for details.This experiment is divided into 5 groups with rat, 8 every group.1. the blank group gives and the normal saline of drug study group with dosage; 2. positive controls gives the medicine imipramine, and dosage is 20mg/kg, and this is a kind of clinical antidepressants with better therapeutical effect that are applied to; 3. experimental group gives the medicine Total Of Triterpenes, is divided into basic, normal, high three dosage groups, and dosage is respectively 30mg/kg, 60mg/kg, 120mg/kg, and each is organized administration time and is the hairy identical time of doing in back 24 hours of rat.Route of administration is divided into once abdominal cavity injection administration and three gastric infusions.(2) the not long-term stress stimulation experiment of precognition: adopting not, the long-term stress stimulation of precognition can make animal produce depression, that is laboratory animal is accepted various stimulation in 21 days usually, comprise electric shock vola, cold water swimming, thermostimulation, rock, press from both sides tail, taboo water, fasting and stimulation such as put upside down round the clock, just can produce depression.Method sees " modern pharmacology experimental technique " (the same) for details.This laboratory animal is grouped into, 1. blank group, normal rat does not give any stimulation, give with the drug study group with the dosage distilled water; 2. depression model group, normal rat give to stimulate in 21 days but do not give any medicine, observe the situation that it produces depression; 3. positive controls; 4. experimental group, the gives stimulation after 3., 4. group is the rat administration again, and medicine and dosage are tested with forced swimming, observe the preventive and therapeutic effect of medicine to depression.Route of administration is for irritating stomach.1) preceding 1 day of experiment and experiment beginning back are the 22nd day, measure rat body weight and the amount of drinking of 1% sucrose solution in 24 hours, the relatively variation of its weight increase amount and the sucrose solution amount of drinking.2) spacious case activeness experiment: preceding 1 day respectively in experiment, the the 7th, 14 and 21 day of experiment beginning, rat is placed high 40 centimetres, 80 centimetres of diameters, perisporium is a black, in the spacious case of the cylinder that 25 lattice that the bottom surface is equated by area are formed, illumination is 60 watts, the behavior of record rat, to pass through bottom surface lattice number is the horizontal anomalous movement score, upright number of times is the score that moves both vertically, and measures reason hair time, waiting time and defecation frequency simultaneously, and minute is 3 minutes.Method sees " modern pharmacology experimental technique " (the same) for details.3) observe the variation of respectively organizing rat brain parietal cortex and hippocampal neuron form from histopathologic slide, relatively the difference between each group.Method sees " histopathologic slide's technology " (height is engraved all, the Huang Yilu work, and hall publishing house in South Mountain publishes, and 1988:124, Nissl staining prepare rat brain nervous tissue pathological section) for details.4) experiment is the 22nd day, adopts to put the method for exempting from and measure thyroliberin (ACTH) in the rat blood serum and thyrotropin (TSH), the trilute (T in the blood plasma
3), thyroxine (T
4), 3,3,5-trilute (rT
3) secretion, observe the function effect of Total Of Triterpenes to rat hypothalamus-hypophysis-adrenal cortex axle and hypothalamus-hypophysis-hypothalamic pituitary thyroidal axis.3, experimental result 1) the forced swimming experimental result sees Table 3
Table 3 is respectively organized the variation of rat forced swimming dead time
Group intraperitoneal injection non-swimming time gastric infusion non-swimming time
(second) (second)
Blank group 175.25 ± 9.50 153.38 ± 10.17
Positive controls 107.50 ± 13.36
*41.25 ± 17.68
*Total Of Triterpenes low dose group 161.67 ± 16.46 124.88 ± 11.64
*Dosage group 153.38 ± 21.17 in the Total Of Triterpenes
*92.63 ± 13.26
*Total Of Triterpenes high dose group 152.50 ± 24.64
*45.88 ± 9.35
*
Compare with the blank group,
*P＜0.05,
*P＜0.01
By result in the table 3 as can be seen, the once abdominal cavity injection administration, the middle and high dosage group of Total Of Triterpenes reduces the forced swimming dead time more significantly, and is though low dose group has also reduced the forced swimming dead time, not remarkable.Gastric infusion, Total Of Triterpenes not only significantly reduce the forced swimming dead time, and high, medium and low dosage group has also shown certain dose dependent relation.Show that Total Of Triterpenes has the effect of control animal depression.2) the long-term stress stimulation experimental result of precognition does not see Table 4
The rat body weight recruitment respectively organized by table 4 and 1% sucrose is drunk quantitative changeization
Group weight increase amount 1% sucrose solution is drunk quantitative changeization (milliliter)
The 22nd day blank matched group 122.90 ± 8.57 38.63 ± 4.58 40.56 ± 3.91 of (gram) experiment experiment in preceding 1 day
*Depression model group 46.35 ± 5.75 40.50 ± 3.25 13.81 ± 3.86 positive controls 99.98 ± 12.31
*39.38 ± 2.97 31.94 ± 6.57
*Dosage group 74.48 ± 10.73 in Total Of Triterpenes low dose group 53.06 ± 9.13 39.75 ± 3.21 18.63 ± 6.88 Total Of Triterpenes
*36.38 ± 4.18 19.56 ± 8.14 Total Of Triterpenes high dose group 86.11 ± 8.71
*40.25 ± 4.36 23.00 ± 8.38
*Compare with the depression model group
*P＜0.05,
*P＜0.01
As can be seen from Table 4, depression model group rat body weight recruitment and the 1% sucrose solution amount of drinking have extremely significantly decline than the blank group, show that the model that we adopt makes rat be in depressive state; Positive controls changes tool to above-mentioned two kinds and has a better role, and illustrates that the antidepressants imipramine has shown the obvious treatment effect in this model; The Total Of Triterpenes low dose group does not have obvious change to the recruitment of depression the weight of animals and the amount of drinking of sucrose solution, the middle and high dosage group of Total Of Triterpenes has more significantly increased body weight and the 1% aqueous sucrose solution amount of drinking of animal, shows that the high dose Total Of Triterpenes has preventive and therapeutic effect to the animal depression.
Can observe from spacious case experiment, the minimizing of depression model group motor activity comprises the rat horizontal anomalous movement, moves both vertically and manages the minimizing of mao time, and the increase of waiting time and times of defecation shows that the model that we adopt makes rat be in depressive state; Positive controls significantly increases the activity of depression rat, not only shows imipramine to depression therapeutical effect preferably, and shows that it has preferably with reference to property as positive control; The Total Of Triterpenes low dose group does not improve significantly to the depressive state of animal, the middle and high dosage group of Total Of Triterpenes has then shown effect more significantly to the change of 5 kinds of behaviors more than the depressed rat, comprising horizontal anomalous movement after 1 week, move both vertically and manage the increase gradually of mao time, the minimizing gradually of waiting time and times of defecation shows that the middle and high dosage of Total Of Triterpenes has therapeutical effect preferably to the laboratory animal depression.3) variation of respectively organizing rat brain parietal cortex and hippocampal neuron form is observed from the Nissl of rat brain histopathologic slide coloration result:
Blank group rat parietal cortex neuron Nissl body is clear, the neurocyte marshalling, and layering is clear, visible from outside to inside molecular layer, external granular layer, external pyramidal layer, internal granular layer, internal pyramidal layer and multi-layer cellular layer.Hippocampus CA1, CA3 and dentate gyrus neuron Nissl body are clear, the neurocyte marshalling, and level is more, and neuron morphology is complete.
Depression model group rat parietal cortex neuron Nissl body reduces, the neurocyte arrangement disorder, and layering is unclear, and is obvious with external pyramidal layer, internal granular layer, internal pyramidal layer cellular change especially.Hippocampus CA1, CA3 and dentate gyrus neuron Nissl body are unintelligible, and neurocyte is arranged mixed and disorderly, and level is less, and visible more neurocyte has vacuolation and karyopycnosis, and the part cell detachment has notable difference with the blank group.
Positive controls rat parietal cortex neuron Nissl body is more clear, and neurocyte is arranged more neat, and layering is clearer, visible from outside to inside molecular layer, external granular layer, external pyramidal layer, internal granular layer, internal pyramidal layer and multi-layer cellular layer.Hippocampus CA1, CA3 and dentate gyrus neuron Nissl body are more clear, and neurocyte is arranged more neat, and level is many than model group, and neuron morphology is more complete, and the fraction cell detachment is arranged.
Each dosage group rat parietal cortex neuron Nissl body of Total Of Triterpenes is more clear, and neurocyte is arranged more neat, and layering is clear, visible from outside to inside molecular layer, external granular layer, external pyramidal layer, internal granular layer, internal pyramidal layer and multi-layer cellular layer.Hippocampus CA1, CA3 and dentate gyrus neuron Nissl body are more clear, and neurocyte is arranged more neat, and neuron morphology is more complete, and neurocyte has vacuolation, and the fraction cell detachment is arranged, and have compared with the depression model group more significantly and have improved.
From the section result find out Total Of Triterpenes each the group improved depressed animal brain cortex, Hippocampus and phenomenons such as dentate gyrus neuron arrangement disorder, karyopycnosis and cavityization, show that Total Of Triterpenes has certain preventive effect to the pathological change of depressed rat cerebral tissue form aspect.4) respectively organize the rat hormonal change and the results are shown in Table 5
Table 5 is respectively organized rat hormonal change group ACTH TSH T
3T
4RT
3
(mg/L) (mU/L) (nmol/L) (nmol/L) (mmol/L) blank group 0.726 ± 0.094
*0.288 ± 0.069
*0.96 ± 0.17
*84.27 ± 16.58
*0.243 ± 0.025
*Depression model group 0.242 ± 0.049 0.078 ± 0.016 1.31 ± 0.18 52.40 ± 11.55 0.145 ± 0.019 positive controls 0.594 ± 0.234 0.202 ± 0.036
*1.00 ± 0.17
*77.41 ± 5.31
*0.195 ± 0.024
*Total Of Triterpenes low dose group 0.411 ± 0.092
*0.168 ± 0.025
*0.82 ± 0.38
*61.18 dosage group 0.476 in ± 15.44 0.159 ± 0.052 Total Of Triterpenes ± 0.210 0.186 ± 0.022
*1.01 ± 0.23 73.38 ± 7.08
*0.179 ± 0.047 Total Of Triterpenes high dose group 0.478 ± 0.152
*0.146 ± 0.015
*0.96 ± 0.17
*82.70 ± 6.31
*0.184 ± 0.018
*Compare with the depression model group
*P＜0.05,
*P＜0.01
As can be seen from Table 5, compare depression model group rat ACTH, TSH, T with blank group rat
4And rT
34 endocrine indexes significantly reduce, and T
3Then obviously increase, show that the model that we adopt makes the rat endocrine dysfunction; Positive controls has been improved depression rat endocrine regulation preferably; Each dosage group increase in various degree of Total Of Triterpenes ACTH, TSH, T
4And rT
3Secretion, reduce T
3Secretion, show that Total Of Triterpenes improves the endocrine disorder of depression rat preferably, has antidepressant effect.
Embodiment 1
To soak 48 hours in 1 kilogram of exsiccant Herba Centellae of 8 liter of 70% ethanol water adding, with the extracting solution decompression recycling ethanol, obtain 2.5 liters of spissated extracting solution, freezing precipitation, filtration under diminished pressure, remove precipitation, the D101 type Saponin that kilogram pretreatment of filtrate application of sample to 0.5 is good adsorbs on the macroporous resin column, removes impurity with 2 liters of distilled water eluting earlier, become light to the eluent color, with 3 liter 70% ethanol water eluting Total Of Triterpenes, collect ethanol elution, eluent is evaporated to dried, with 250 milliliters of analytical pure dissolve with methanol, the crystallization of 2 liters of analytical pure ethyl acetate of reuse in 45 ℃ of vacuum dryings, obtains 19.6 gram pale yellow powder shape solids.
Embodiment 2
To soak 54 hours in 2 kilograms of exsiccant Herba Centellaes of 16 liter of 75% ethanol water adding, with the extracting solution decompression recycling ethanol, obtain 5 liters of spissated extracting solution, freezing precipitation, filtration under diminished pressure, remove precipitation, the ZTC-1 type Saponin that kilogram pretreatment of filtrate application of sample to 1 is good adsorbs on the macroporous resin column, removes impurity with 4 liters of distilled water eluting earlier, become light to the eluent color, with 8 liter 70% ethanol water eluting Total Of Triterpenes, collect eluent, eluent is evaporated to dried, with 400 milliliters of analytical pure dissolve with methanol, the crystallization of 3 liters of analytical pure ethyl acetate of reuse in 45 ℃ of vacuum dryings, obtains 29.8 gram pale yellow powder shape solids.
Embodiment 3
To soak 58 hours in 5 kilograms of exsiccant Herba Centellaes of 50 liter of 75% ethanol water adding, the extracting solution decompression recycling ethanol, obtain 12 liters of spissated extracting solution, freezing precipitation, filtration under diminished pressure, the ZTC-1 type Saponin that kilogram pretreatment of filtrate application of sample to 2 is good adsorbs on the macroporous resin column, remove impurity with 7 liters of distilled water eluting, become to the eluent color light, with 10 liter 75% ethanol water eluting Total Of Triterpenes, collect eluent, eluent is evaporated to dried, with 500 milliliters of analytical pure dissolve with methanol, the crystallization of 4 liters of analytical pure ethyl acetate of reuse, in 45 ℃ of vacuum dryings, obtain 105 gram yellow powder shape solids.
Advantage of the present invention and good effect:
Total Of Triterpenes preparation technology of the present invention is simple, has not only shortened technological process, has improved yield, has reduced one-tenth This has reduced environmental pollution, and Total Of Triterpenes has been used for the treatment of depression, might provide one for patients with depression Plant effective natural drug.
Claims (2)
1, a kind of method for preparing Total Of Triterpenes, extraction, purification and three steps of crystallization of comprising Total Of Triterpenes, the purification that it is characterized in that Total Of Triterpenes adopts Saponin absorbent-type macroporous resin, that is the Total Of Triterpenes extracting solution is by this resin absorption extract, remove impurity through the distilled water eluting, get the Total Of Triterpenes eluent with the aqueous alcohol eluting, the concentrating under reduced pressure eluent is to doing, with after the aqueous alcohol dissolving, use the ethyl acetate crystallization again.
2, Total Of Triterpenes is in order to the purposes of treatment depression.
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| CN1313096C (en) * | 2004-06-22 | 2007-05-02 | 神威药业有限公司 | Asiaticoside use of medicinal preparation for treating and preventing chronic renal failure |
| CN101652143A (en) * | 2007-02-12 | 2010-02-17 | Indus生物技术私人有限公司 | Be used for compositions and method thereof that the selectivity serotonin reuptake transporter suppresses |
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| CN101396384B (en) * | 2007-09-28 | 2012-11-21 | 天津天士力现代中药资源有限公司 | Asiatic centella extract and preparation methode thereof |
| CN101200487B (en) * | 2007-11-27 | 2010-09-29 | 浙江大学 | Method for preparing asiatic centella total saponins by using macroporous adsorption resin |
| CN101323637B (en) * | 2008-07-29 | 2010-12-22 | 卢照凯 | Asiaticoside and preparation thereof |
| CN101407536B (en) * | 2008-11-27 | 2011-05-18 | 浙江大学 | Process for preparing high-purity asiaticoside by solvent crystallization |
| CN102443036A (en) * | 2010-10-09 | 2012-05-09 | 苏州宝泽堂医药科技有限公司 | Method for purifying asiatic acid in asiatic pennywort herb |
| CN102617696A (en) * | 2012-03-16 | 2012-08-01 | 广州汉方现代中药研究开发有限公司 | Preparation method of asiaticoside |
| CN102617696B (en) * | 2012-03-16 | 2014-10-22 | 广州白云山汉方现代药业有限公司 | Preparation method of asiaticoside |
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