CN1366813A - Method cultivating rat with idiopathic arterial pressure sensitivity reflex function defect - Google Patents
Method cultivating rat with idiopathic arterial pressure sensitivity reflex function defect Download PDFInfo
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- CN1366813A CN1366813A CN 02110894 CN02110894A CN1366813A CN 1366813 A CN1366813 A CN 1366813A CN 02110894 CN02110894 CN 02110894 CN 02110894 A CN02110894 A CN 02110894A CN 1366813 A CN1366813 A CN 1366813A
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Abstract
The present invention selects and uses hereditary hypertension rat and rat with normal blood pressure to make hybridization, and the rat whose blood pressure is normal and arterial pressure susceptivity reflection sensitivity is less than 0.3 ms/mm Hg can be screened out from filial generation, bred and propagated, and repeatedly bred and propagated, after above 12 generations so as to obtain the invented pure rat with stable hereditary property and spontaneous arterial pressure susceptivity reflection functional defect. The rat obtained by said invention can be used for further researching relationship of arterial pressure susceptivity reflection and cardiovascular disease and can be used for improving development of reflection function medicine.
Description
Technical field: the present invention relates to medical experiment animal model technical field, is the method for cultivating the purebred rat of a kind of idiopathic arterial pressure sensitivity reflex function defect that is used for the angiocardiopathy study on prevention.
Background technology: (arterial baroreflex ABR) is the most important regulation mechanism of cardiovascular activity to arterial baroreceptor reflex.In recent years, the relation of ABR dysfunction and angiocardiopathy is subject to the people's attention.1988, La Rovere etc. 78 male sex's myocardial infarction patients show effect for the first time the back around measured arterial baroreflex sensitivity (baroreflex sensitivity, BRS).Through 24 months follow up a case by regular visits to, 6 death are arranged.The BRS that found that dead person is lower than the survivor significantly.Zoopery also obtains similar result: this breadboard Schwartz etc. with the branch of coronary artery ligation of 192 dogs to cause the antetheca myocardial infarction, 4-5 week is measured BRS after infarct, block the coronary artery circumflex branch subsequently more momently, wherein there are 106 dog generating chamber to quiver, their BRS is lower than the dog that generating chamber not quivers significantly, and the easy dog that quivers of generation chamber, its BRS low than other animal just before the descending anterior branch ligation.La Rovere in 1998 etc. have reported the once more massive research of their tissue again, have observed 1284 patients, have obtained similar result.
The inventor has carried out a large amount of research to the relation of ABR function and organ damage.With the afferent nerve excision of ABR, (sinoaortic denervation SAD), studies the relation of ABR function and organ damage to be called the Dou Gong nerve.Found that the performance of a series of organ damages such as myocardial hypertrophy, nephridial tissue damage and vascular remodeling appears in the SAD rat.Also finding has very close correlation between the organ damage of the function of ABR and spontaneous hypertensive rat (SHR).As seen the ABR hypofunction is extremely important in angiocardiopathy.
Along with medical science applied development, the research of numerous disease or syndrome there is its corresponding animal pathological model.For example, to the research of high blood pressure, spontaneous hypertensive rat SHR etc. is arranged as animal model; To the research of diabetes, corresponding inheritance diabetes rat is arranged as animal model, or the like.And research ABR hypofunction does not still have animal model so far, and what can use at present is to remove Dou Gong nerve (SAD) with operating method, causes the ABR functional lesion, studies again.Clearly, SAD can't become the ideal animal model of research ABR hypofunction.And ideal animal model should be the animal model of a kind of inheritance, spontaneous ABR hypofunction.
Summary of the invention: the present invention is the breeding method of a kind of rat of heritable spontaneous ABR functional defect.The rat that the present invention cultivates can be used as the animal model of research ABR hypofunction.ABR functional defect major part sees and will obtain the day after tomorrow, can cause the ABR hypofunction as hypertension.But also the someone reported SHR before elevation of blood pressure, and going down of ABR function just arranged.Also have report, hypertensive patient's the next generation also had going down of ABR function before elevation of blood pressure.These illustrate that all the ABR function has certain inheritance.As long as the ABR functional defect has inheritance, in theory just should be able to cultivate the animal model of spontaneous ABR functional defect.
The inventor is in close relations according to spontaneous hypertensive rat and ABR hypofunction, thus select for use Hypertensive Rats SHR and normotensive common purebred rat SD (Sprague-Dawley) to hybridize, again from its filial generation screening.This is because the ABR hypofunction major part of SHR is influenced by hypertension, also has fraction to come from inherent cause.With SHR and the hybridization of SD rat, separate from hypertension for this part ABR functional defect that will wherein be subjected to inherent cause control exactly.In the filial generation of hybrid animal, it is normal and ABR hypofunction person further cultivates to select blood pressure, selects so again, breeding again, after some generations, just can cultivate into purebred ABR functional defect rat.This rat model is named as rat with idiopathic arterial pressure sensitivity reflex function defect (arterialbaroreflex-deficientrat is called for short ABR-DR).ABR-DR is that blood pressure is normal and the hypokinetic animal of ABR has very desirable genetic stability.This animal model can be used for studying ABR role in various angiocardiopathies; Research can improve the medicine of ABR function; And the genome of further research decision ABR function.
Embodiment: the concrete grammar of cultivating ABR-DR is as follows:
With 10 male SHR and 20 female sd inbred rats hybridization, about 120 of the F1 filial generation of generation.In addition with 10 SD male rats and 20 SHR female rats hybridization, about 80 of the F1 filial generation of generation.With about these 200 F1 filial generations as a colony.To have height to have low for blood pressure in the middle of their, the ABR function have have by force a little less than.BRS is as the criterion with the reflection function index, with blood pressure normal (＜140/90mmHg) and the F1 filial generation rat of BRS below 0.3ms/mmHg selected, and breed, the rat that bears is named as ABR-DR (the 1st generation).Again by same standard, promptly normal, the BRS of blood pressure is lower than 0.3ms/mmHg, screens, breeds, and produces the second generation in the 1st generation, and the like.More than 12 generations of repeated screening in this way, breeding, just can obtain the purebred rat of idiopathic arterial pressure sensitivity reflex function defect of genetic stability.As the contrast strain of ABR-DR, when cultivating ABR-DR, filter out blood pressure rat normal, that BRS is higher than 0.7ms/mmHg from the F1 filial generation and breed the same period.The offspring that these animals produce is named as ABR-NR (the arterial baroreflex-normal rats first generation).Screen, breed more than 12 generations by same standard repeatedly, get the purebred rat of ABR-NR, its genetic stability, special in the contrast strain of studying ABR-DR.
Claims (5)
1. the breeding method of rat with idiopathic arterial pressure sensitivity reflex function defect, it is characterized in that selecting for use the hybridization of genetic hypertension rat and normal arterial pressure rat, from filial generation, filter out blood pressure rat normal, that arterial baroreflex sensitivity is lower than 0.3ms/mmHg and carry out ectogenesis, and in its offspring, screen, breed with this standard repeatedly, cultivate more than 12 generations, just can obtain the rat with idiopathic arterial pressure sensitivity reflex function defect of genetic stability.
2. by the breeding method of the described rat with idiopathic arterial pressure sensitivity reflex function defect of claim 1, it is characterized in that said genetic hypertension rat is SHR.
3. by the breeding method of claim 1 or 2 described rat with idiopathic arterial pressure sensitivity reflex function defect, it is characterized in that described normal arterial pressure rat is the SD rat.
4. claim 1,2 described rat with idiopathic arterial pressure sensitivity reflex function defect breeding method gained rats are in order to the relation of research arterial pressure sensitivity reflex function and angiocardiopathy.Development improves the purposes of reflection function medicine.
5. the described rat with idiopathic arterial pressure sensitivity reflex function defect breeding method of claim 3 gained rat in order to the relation of research arterial pressure sensitivity reflex function and angiocardiopathy, is developed the purposes of improving the reflection function medicine.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB021108943A CN1168382C (en) | 2002-02-26 | 2002-02-26 | Method cultivating rat with idiopathic arterial pressure sensitivity reflex function defect |
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| CNB021108943A CN1168382C (en) | 2002-02-26 | 2002-02-26 | Method cultivating rat with idiopathic arterial pressure sensitivity reflex function defect |
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| CN1366813A true CN1366813A (en) | 2002-09-04 |
| CN1168382C CN1168382C (en) | 2004-09-29 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111418553A (en) * | 2020-04-16 | 2020-07-17 | 段为钢 | Hyperuricemia rat model and construction method thereof |
| CN115251007A (en) * | 2022-09-02 | 2022-11-01 | 山西医科大学 | New strain naked rat, and cultivation method and application thereof |
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2002
- 2002-02-26 CN CNB021108943A patent/CN1168382C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111418553A (en) * | 2020-04-16 | 2020-07-17 | 段为钢 | Hyperuricemia rat model and construction method thereof |
| CN115251007A (en) * | 2022-09-02 | 2022-11-01 | 山西医科大学 | New strain naked rat, and cultivation method and application thereof |
| CN115251007B (en) * | 2022-09-02 | 2024-01-26 | 山西医科大学 | New-line nude rat, and cultivation method and application thereof |
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| CN1168382C (en) | 2004-09-29 |
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