CN1358865A - Method for accurate identifying eight-digit code of various kinds of baterium of enterobacter - Google Patents
Method for accurate identifying eight-digit code of various kinds of baterium of enterobacter Download PDFInfo
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- CN1358865A CN1358865A CN 00129411 CN00129411A CN1358865A CN 1358865 A CN1358865 A CN 1358865A CN 00129411 CN00129411 CN 00129411 CN 00129411 A CN00129411 A CN 00129411A CN 1358865 A CN1358865 A CN 1358865A
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Abstract
The 8-bit code accurate determination method of various bacteria of enteric bacilli includes the following steps: ordering 24 biochemical reactions of indole, methyl red and others which can act on various bacteria of enteric bacilli, dividing them into 8 groups and making standard reagent strips, according to the positive or negative result produced by every biochemical reaction to act on some bacterium, forming 8-bit standard code, then centralizing all the standard codes to make standard code book or computer application program to implement accurate determination.
Description
Each belongs to kind of a bacteria analysis authentication method, the quick method for accurate identifying of specifically a kind of eight-digit code of various kinds of baterium of enterobacter to the present invention relates to a kind of enterobacteria.
Manual Analysis and Identification is adopted in evaluation in the medical circle microorganism for many years always, also have and use the microorganism identification instrument to do to identify, but existing deficiency is that identification equipment costs an arm and a leg in application process, the cost of use height, make a Bacteria Identification expense about 40-80 unit, strengthened patient's medical treatment burden, each medical institutions both domestic and external still adopt manual each of carrying out enterobacteria to belong to the evaluation of kind of bacterium.With regard to bacterium is planted in craft to each genus of enterobacteria authentication method, in use existing deficiency is a poor accuracy, the problem of omission or flase drop happens occasionally, and has had a strong impact on medical worker's correct treatment, brings spiritual misery and loss economically to the patient.
The enterobacteria of prior art each to belong to kind of 8 figure place method for determining bacteria be to select enterobacteria each to belong to biochemical reaction that kind of bacterium the works numbering that sorts, per in order then three kinds of biochemical reactions are one group and divide into groups successively to arrange, the lower-order digit of every group of biochemical reaction sequence code is 4, next is 2, last is 1, form 421 basis numberings with this, in the Bacteria Detection process, can be positive in every group with the biochemical reaction that bacterium is worked, inoperative negative, the basis that shows the biochemical reaction of feminine gender is numbered 0, it is constant to show male basis numbering, to show feminine gender or the male basis packet numbering numbering addition and that be this group biochemical reaction, use many group biochemical reactions to carry out analyzing and testing and just can draw a plurality of packet numberings, a plurality of packet numberings are arranged in the standard numbering of just forming this bacterium together for a certain bacterium.According to aforesaid method, use the biochemical reaction of serialization grouping that the standard model that enterobacteria respectively belongs to all bacteriums in kind of bacterium is carried out analyzing and testing, each belongs to the standard code basis of all bacteriums in kind bacterium just to obtain enterobacteria, all bacterium standard codes are originally made benchmark in each genus kind of bacterium of use enterobacteria, just all these contrasts of bacterium standard code in each genus kind of bacterium of bacterial identification that clinical detection can be gone out and enterobacteria, be that any germ is being caused a disease in the patient of the place of the looking for body, the doctor just can suit the remedy to the case exactly, and treatment is played a multiplier effect.
At present, the biochemical reaction of each selected use of hospital both at home and abroad is different, thereby the method for ordering grouping also is different, domestic each hospital is employed to have only 5 figure places to each genus of enterobacteria kind of Bacteria Detection standard code, also can only reach 7 figure places abroad, obviously, the coding figure place is high more, the precision that detects is high more, and omission and false drop rate are low more.Invention and use higher figure place enterobacteria each belong to kind of bacterial analysis and detect the height that coding techniques then reflects this field medical science level.
The purpose of this invention is to provide an a kind of eight-digit code of various kinds of baterium of enterobacter method for accurate identifying that is used for each genus of enterobacteria kind of bacterium Rapid identification.
The objective of the invention is to realize in the following manner, adopt 24 kinds of biochemical reactions commonly used (following will " reaction: the omission of two words): indoles; methyl red; V-P; citrate; hydrogen sulfide; urea, phenylalanine, Methionin, ornithine, malonate, lactic acid, sucrose, N.F,USP MANNITOL, inositol, sorbyl alcohol, raffinose, gill fungus sugar, the β semi-lactosi, rhamnosyl, maltose, Pentitol, glucose produces acid, the glucose aerogenesis, each belongs to kind of bacteria analysis and identifies that a biochemical reaction carries out a science ordering power as enterobacteria, and putting in order of above-mentioned biochemical reaction is 1 indoles, 2 methyl reds, 3V-P, 4 citrates, 5 hydrogen sulfide, 6 urea, 7 phenylalanines, 8 Methionins, 9 ornithines, 10 malonates, 11 lactic acid, 12 sucrose, 13, N.F,USP MANNITOL, 14 inositols, 15 sorbyl alcohols, 16 raffinoses, 17 gill fungus sugar, 18 β semi-lactosis, 19 rhamnosyls, 20 maltose, 21 Pentitols, 22 glucose produce acid, 23 glucose aerogenesis, 24 power.
The eight-digit code of various kinds of baterium of enterobacter method for accurate identifying is characterized in that being grouped into of above-mentioned biochemical reaction:
Basic number of group number biochemical reaction title
First group of 1 indoles 2 methyl reds 3 V-P 421
Second group of 4 citrate 5 hydrogen sulfide 6 urea 421
The 3rd group: 7 phenylalanines, 8 Methionins, 9 ornithines 421
The 4th group: 10 malonates, 11 lactic acid, 12 sucrose 421
The 5th group: 13 N.F,USP MANNITOL, 14 inositols, 15 sorbyl alcohols 421
The 6th group: 16 raffinoses, 17 gill fungus sugar, 18 β semi-lactosis 421
The 7th group: 19 rhamnosyls, 20 maltose, 21 Pentitols 421
The 8th group: 22 glucose produce sour 23 glucose aerogenesis, 24 power 421
The preparation method of biochemical reaction reagent is: the reagent of 1-21 kind biochemical reaction is injected the reagent strip of being with number container, and reaching through aseptic dehydration rear enclosed is product, and back 3 kinds reagent is instant preparation.
Embodiment: eight-digit code of various kinds of baterium of enterobacter method for accurate identifying of the present invention, application very simple and convenient, to sort earlier: 1 indoles, 2 methyl reds, 3V-P, 4 citrates, 5 hydrogen sulfide, 6 urea, 7 phenylalanines, 8 Methionins, 9 ornithines, 10 malonates, 11 lactic acid, 12 sucrose, 13, N.F,USP MANNITOL, 14 inositols, 15 sorbyl alcohols, 16 raffinoses, 17 gill fungus sugar, 18 β semi-lactosis, 19 rhamnosyls, 20 maltose, 21 Pentitols, 22 glucose produce acid, 23 glucose aerogenesis, 24 kinds of biochemical reactions of 24 power are divided into 8 groups, every group three kinds, the bacterium standard model that each genus of enterobacteria is planted is identified then, according to each bacteria sample each reaction result of organizing biochemical reaction is obtained the standard numbering, the order of standard being numbered by group number is arranged in the standard code that promptly obtains each genus kind of bacterium together again, and the standard code of last various bacteriums is corresponding with its title makes each genus of enterobacteria kind of bacterium 8 figure place standard codes originally.Need only during use and use 8 groups of biochemical reactions can obtain the coding of one group of 8 figure place, as long as on the standard code basis, find identical coding can find and this corresponding bacterium of encoding the sample analysis that any one contains bacterium.If this standard code is input to makes a file or program in the computer,, apace sample is identified as long as utilize the query function of computer just can inquire about each bacterium that belongs to kind easily simply.For example: the sample result of unknown bacterium is as shown in the table: unit: name: sex: the age: sample number into spectrum: identify the date on date
| The reagent sequence number | 1 | 2 | 3 | 4 | 5 | 6 | ?7 | ?8 | ?9 | ?10 | 11 | ?12 | ?13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | ?23 | ?24 |
| Reagent name | Indoles | Methyl red | V I P | Citrate | Hydrogen sulfide | Urea | Phenylalanine | Methionin | Ornithine | Malonate | Lactic acid | Sucrose | N.F,USP MANNITOL | Inositol | Sorbose | Raffinose | The gill fungus breast | β half sugar | Rhamnosyl | Maltose | Pentitol | Glucose produces acid | The glucose aerogenesis | Power |
| Qualification result | 4 | ?2 | ?1 | ?4 | ?2 | ?1 | ?4 | ?2 | ?1 | ?4 | ?2 | ?1 | ?4 | ?2 | ?1 | ?4 | ??2 | 1 | ?4 | ?2 | ?1 | ?4 | ?2 | ?1 |
| - | ?+ | ?- | ?- | ?+ | ?- | ?- | ?+ | ?- | ?- | ?- | ?- | ?+ | ?- | ?+ | ?- | ??+ | - | ?- | ?+ | ?- | ?+ | ?- | ?+ | |
| ??????2 | ??????2 | ??????2 | ??????0 | ??????5 | ??????2 | ?????2 | ??????5 | |||||||||||||||||
| Bacterial identification: | ????22205225 | |||||||||||||||||||||||
| Bacteria name: | Salmonella typhi | |||||||||||||||||||||||
Because the standard code of salmonella typhi on the standard code basis is 22205225, so the bacterium in the sample is a salmonella typhi.
The preparation method of biochemical reaction reagent is: the reagent of 1-21 kind biochemical reaction is injected the reagent strip of being with number container, and being reached by rear enclosed through aseptic dehydration bag is product, and back 3 kinds reagent is instant preparation.
Above-mentioned 24 kinds of biochemical reactions and agents useful for same are biochemical reaction commonly used and common reagent.
Eight-digit code of various kinds of baterium of enterobacter method for accurate identifying of the present invention is compared with prior art, have reasonable in design, easy to use, identify the precision height, identify quick characteristics such as rapid, thereby, have good value for applications.
Claims (3)
1. eight-digit code of various kinds of baterium of enterobacter method for accurate identifying, comprise the ordering of biochemical reaction, the preparation of grouping and biochemical reaction reagent is characterized in that the ordering of biochemical reaction is: 1 indoles, 2 methyl reds, 3V-P, 4 citrates, 5 hydrogen sulfide, 6 urea, 7 phenylalanines, 8 Methionins, 9 ornithines, 10 malonates, 11 lactic acid, 12 sucrose, 13, N.F,USP MANNITOL, 14 inositols, 15 sorbyl alcohols, 16 raffinoses, 17 gill fungus sugar, 18 β semi-lactosis, 19 rhamnosyls, 20 maltose, 21 Pentitols, 22 glucose produce acid, 23 glucose aerogenesis, 24 power.
2. eight-digit code of various kinds of baterium of enterobacter method for accurate identifying according to claim 1 is characterized in that being grouped into of biochemical reaction:
Basic number of group number biochemical reaction title
First group of 1 indoles 2 methyl reds 3 V-P 421
Second group; 4 citrates, 5 hydrogen sulfide, 6 urea 421
The 3rd group: 7 phenylalanines, 8 Methionins, 9 ornithines 421
The 4th group: 10 malonates, 11 lactic acid, 12 sucrose 421
The 5th group: 13 N.F,USP MANNITOL, 14 inositols, 15 sorbyl alcohols 421
The 6th group: 16 raffinoses, 17 gill fungus sugar, 18 β semi-lactosis 421
The 7th group: 19 rhamnosyls, 20 maltose, 21 Pentitols 421
The 8th group: 22 glucose produce sour 23 glucose aerogenesis, 24 power 421
3. eight-digit code of various kinds of baterium of enterobacter method for accurate identifying according to claim 1, the preparation method who it is characterized in that biochemical reaction reagent is: the reagent strip that the reagent of 1-21 kind biochemical reaction is injected the band number container, reaching through aseptic dehydration rear enclosed is product, and back 3 kinds reagent is instant preparation.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB001294113A CN1134543C (en) | 2000-12-13 | 2000-12-13 | Method for accurate identifying eight-digit code of various kinds of baterium of enterobacter |
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| CNB001294113A CN1134543C (en) | 2000-12-13 | 2000-12-13 | Method for accurate identifying eight-digit code of various kinds of baterium of enterobacter |
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| CN1358865A true CN1358865A (en) | 2002-07-17 |
| CN1134543C CN1134543C (en) | 2004-01-14 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103760159A (en) * | 2014-01-24 | 2014-04-30 | 山东鑫科生物科技股份有限公司 | Method and system for identifying bacteria and analyzing drug sensitivity |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103760159A (en) * | 2014-01-24 | 2014-04-30 | 山东鑫科生物科技股份有限公司 | Method and system for identifying bacteria and analyzing drug sensitivity |
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