CN1357618A - Biological bacteriophagic Bdellovibrio prepn and its production process - Google Patents
Biological bacteriophagic Bdellovibrio prepn and its production process Download PDFInfo
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- CN1357618A CN1357618A CN00129401A CN00129401A CN1357618A CN 1357618 A CN1357618 A CN 1357618A CN 00129401 A CN00129401 A CN 00129401A CN 00129401 A CN00129401 A CN 00129401A CN 1357618 A CN1357618 A CN 1357618A
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- biological
- bacteriophagic bdellovibrio
- preparation
- substratum
- bacteriophagic
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Abstract
The present invention belongs to the field of biological technology and the biological bacteriophagic Bdellovibrio preparation is produced through a no-host bacteria process. The preparation of the present invention has low production cost and high use effect.
Description
The invention belongs to biological product technical field, relate to a kind of biological bacteriophagic Bdellovibrio preparation and and production method thereof.
Biological bacteriophagic Bdellovibrio preparation in the prior art, as the disclosed application number of Patent Office of the People's Republic of China is 93111749., 6 patent applications, the patent application document that a kind of title is bactericide prepared from biology and production method thereof is disclosed, a kind of biotechnological formulation of bacteriophagic Bdellovibrio production and production method of this biotechnological formulation utilized is provided, and its preparation is the aqua that bacteriophagic Bdellovibrio and host bacterium are made after cultivating; This production method is that Bdellovibrio is produced culture with deactivation host bacterium cultural method, and produces its biotechnological formulation.
The purpose of this invention is to provide a kind of biological bacteriophagic Bdellovibrio preparation and production method thereof, thereby a kind of production cost is low, production efficiency is high biological bacteriophagic Bdellovibrio preparation and production method thereof are provided, to overcome the deficiencies in the prior art.
Biotechnological formulation of the present invention contains: bacteriophagic Bdellovibrio and substratum thereof, substratum mainly are Yeast diffusion juices and albumen freezes and/or meat soup.
Because it is facultative that bacteriophagic Bdellovibrio has, therefore can carry out the preparation of biotechnological formulation with the substratum of no host bacterium.The production method of biological bacteriophagic Bdellovibrio preparation of the present invention is: liquid nutrient medium and bacteriophagic Bdellovibrio with no host bacterium are produced biological bacteriophagic Bdellovibrio preparation together.
Describe preparation of the present invention and production method below in detail
Biotechnological formulation of the present invention is following composition: bacteriophagic Bdellovibrio and substratum thereof and stablizer, substratum is that albumen freezes, Yeast diffusion juice, or Yeast diffusion juice and meat soup, or albumen freezes, Yeast diffusion juice, meat soup, stablizer can be a SODIUM PHOSPHATE, MONOBASIC.This preparation can be an aqua, also can make lyophilized powder, also can cooperate with other useful bacterial strain such as Su Yun bacillus, Bacillus cereus, Bacillus subtilus, streptococcus faecium etc. and make mixed preparation.
Production method of the present invention is:
By fermentation volume is that the inoculum size of 1-2% is put into the fermentor tank that fills substratum, the substratum composition is: albumen freezes 2-10%, Yeast diffusion juice 2-10%, sodium-chlor 2-10%, SODIUM PHOSPHATE, MONOBASIC 0.5-2%, Sodium phosphate dibasic 1-3%, all the other are water, PH is 7-7.6, maintain the temperature at 25-32 degree centigrade, hold-time 15-20 hour, after detecting, sampling promptly makes the aqua of biological bacteriophagic Bdellovibrio preparation, and can make lyophilized powder or make mixed preparation or lyophilized powder with above-mentioned aqua with other useful bacterial strain.
Preparation of the present invention has the advantage that production cost is low, result of use is good, and method of the present invention is simple, easy handling.
Claims (4)
1, a kind of biological bacteriophagic Bdellovibrio preparation, contain following composition: bacteriophagic Bdellovibrio and substratum thereof, substratum composition are that Yeast diffusion juice and albumen freeze.
2, a kind of biological bacteriophagic Bdellovibrio preparation according to claim 1 is characterized in that: also contain stablizer in the preparation, stablizer is SODIUM PHOSPHATE, MONOBASIC and/or Sodium phosphate dibasic.
3, a kind of production method of biological bacteriophagic Bdellovibrio preparation is: liquid nutrient medium and bacteriophagic Bdellovibrio are put into container together, and controlled temperature was cultivated 15-22 hour at 25-32 degree centigrade, promptly made biological bacteriophagic Bdellovibrio preparation.
4, the production method of a kind of biological bacteriophagic Bdellovibrio preparation according to claim 3, it is characterized in that: by fermentation volume is that the inoculum size of 1-2% is put into the fermentor tank that fills substratum, the substratum composition is: albumen freezes 2-10%, Yeast diffusion juice 2-10%, sodium-chlor 2-10%, SODIUM PHOSPHATE, MONOBASIC 0.5-2%, Sodium phosphate dibasic 1-3%, all the other are water, PH is 7-7.6, maintains the temperature at 25-32 degree centigrade, hold-time 15-20 hour, after sampling detects, promptly make the aqua of biological bacteriophagic Bdellovibrio preparation, can make lyophilized powder or make mixed preparation or lyophilized powder with above-mentioned aqua with other useful bacterial strain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00129401A CN1357618A (en) | 2000-12-15 | 2000-12-15 | Biological bacteriophagic Bdellovibrio prepn and its production process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00129401A CN1357618A (en) | 2000-12-15 | 2000-12-15 | Biological bacteriophagic Bdellovibrio prepn and its production process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1357618A true CN1357618A (en) | 2002-07-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN00129401A Pending CN1357618A (en) | 2000-12-15 | 2000-12-15 | Biological bacteriophagic Bdellovibrio prepn and its production process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1357618A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100394926C (en) * | 2006-04-06 | 2008-06-18 | 薛恒平 | Production process of bdellophage preparation |
| CN101845403A (en) * | 2010-04-26 | 2010-09-29 | 杨雄 | Yeast strain and method for preparing phage bdellovibro preparation by using the same |
-
2000
- 2000-12-15 CN CN00129401A patent/CN1357618A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100394926C (en) * | 2006-04-06 | 2008-06-18 | 薛恒平 | Production process of bdellophage preparation |
| CN101845403A (en) * | 2010-04-26 | 2010-09-29 | 杨雄 | Yeast strain and method for preparing phage bdellovibro preparation by using the same |
| CN101845403B (en) * | 2010-04-26 | 2011-09-21 | 杨雄 | Yeast strain and method for preparing phage bdellovibro preparation by using the same |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |