CN1352306A - Establishing medicine screening gene expression data base by utilizing animal models - Google Patents
Establishing medicine screening gene expression data base by utilizing animal models Download PDFInfo
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- CN1352306A CN1352306A CN 00120549 CN00120549A CN1352306A CN 1352306 A CN1352306 A CN 1352306A CN 00120549 CN00120549 CN 00120549 CN 00120549 A CN00120549 A CN 00120549A CN 1352306 A CN1352306 A CN 1352306A
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Abstract
The present invention, establishing medicine screening gene expression bata by utilizing animal models, relates to inspection of effect of medicine on animal gene expression by using the combination of DNA chip and pharmacology, The system provides animal model for medicine screening, high quality mRNA extraction, high efficiency marking and detection, medicine relative gene expression data base and medicine relative enzyme biological information analysis method. The system may be used in the screening and estimation of medicine and destination compound as well as medicine design.
Description
The present invention relates to utilize animal model to set up medicine screening gene expression data base, purpose is a kind of brand-new medicament sifting motion system.
Drug screening is a costly process consuming time.Every new drug that successfully goes on the market needs synthetic 5,000～10,000 new compound, and about 10 years consuming time, expensive multi-billion dollar.This wherein the biological activity specificity screening of lead compound be the most important thing, and screening conditions and information analysis quality are to influence the crucial determinative that drug target is found.Moreover, many drug targets are after paying multi-billion dollar through the research of many decades, because of toxicity before clinical and side effect are failed greatly.The fast development of new combinatorial chemistry, making at synthetic a large amount of compounds of short period of time becomes possibility, and obviously present drug screening method can not be satisfied the demand far away.Therefore, the invention a kind of can shorten drug discovery research cycle and the reduction research expenditure the drug screening instrument extremely important.
Usually pharmacy industry develops into the vitro enzyme system that sets up recently with the animal screening drug target of flood tide, and U.S. Pat 6033860 is by the differential screening drug target of drug target inhibitory enzyme activity.The present invention sets up a kind ofly to determine more rapidly and efficiently that with gene expression profile the variation that acts on the drug target protein matter of genes encoding by drug target learns which gene is come primary dcreening operation that the drug target of pharmacologically active is arranged by the repressed information of drug-induced which gene with a series of lead compound relative specificities and the toxic screening system of drug target.Concrete technical scheme of the present invention is that animal pattern is handled through drug target, sets up the animal model for drug effect genes involved transcription response; Extract its relevant mRNA, mRNA compares with standard animal gene chip of expression spectrum through amplification, sets up animal pattern gene expression profile database.Because the information high-throughput of gene chip provides instrument for utilizing animal pattern gene expression profile database design, screening drug target.
The present invention can also provide the mechanism of every kind of drug target compounds affect expression of target gene.
The inventor is theoretical through studies confirm that according to genome and related science, and the animal pattern gene expression data base is a kind of brand-new high-throughout medicament sifting motion system.
Therefore, the object of the invention provides a kind of brand-new medicinal design and high-flux medicaments sifting system, shortens the drug development research cycle and reduces the drug development research expense.
The object of the invention also is to provide the saved system of toxicological analysis system, medicine bioinformation.
The object of the invention also is to provide preparation production technique of the present invention.
Production technique of the present invention is as follows:
(1) sets up the animal model of specified disease;
(2) foundation is for the animal model of drug target effect genes involved transcription response
(3) mRNA of extraction model animal related tissue;
(4) mRNA or cDNA amplification;
(5) set up the animal pattern gene expression profile;
(6) compare with standard animal gene chip of expression spectrum;
(7) analytical model animal gene express spectra is set up gene expression data base screening drug target.
Production method of the present invention is as follows:
Accompanying drawing 1 is technological process of production figure of the present invention.
Claims (6)
1. set up animal model for drug effect genes involved transcription response.This animal model comprises Live Animals, animal organ, tissue, cell, and medicine comprises known drug and target compound.
2. according to claim 1, wherein animal refers to be used for the animal of pharmacology, toxicological experiment.
3. according to claim 1, set up the animal pattern gene expression profile, this animal pattern gene expression profile comprises to be composed the drug effect related gene expression.
4. according to claim 3, set up animal model and the animal pattern gene expression profile may further comprise the steps:
(1) sets up the animal model of specified disease;
(2) foundation is for the animal model of drug effect genes involved transcription response;
(3) extract its mRNA;
(4) mRNA or cDNA amplification;
(5) compare with standard animal gene chip of expression spectrum;
(6) set up the animal pattern gene expression profile;
(7) analytical model animal gene express spectra is set up gene expression data base.
5. according to claim 1, animal model, animal pattern gene expression profile are used for clinical preceding drug screening.
6. according to claim 1, animal model, animal pattern gene expression profile are used for toxicological analysis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 00120549 CN1352306A (en) | 2000-11-06 | 2000-11-06 | Establishing medicine screening gene expression data base by utilizing animal models |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 00120549 CN1352306A (en) | 2000-11-06 | 2000-11-06 | Establishing medicine screening gene expression data base by utilizing animal models |
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| CN1352306A true CN1352306A (en) | 2002-06-05 |
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| CN 00120549 Pending CN1352306A (en) | 2000-11-06 | 2000-11-06 | Establishing medicine screening gene expression data base by utilizing animal models |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101784895A (en) * | 2007-06-26 | 2010-07-21 | 协乐民公司 | Method for predicting biological systems responses in hepatocytes |
| CN108182346A (en) * | 2016-12-08 | 2018-06-19 | 杭州康万达医药科技有限公司 | Predict method for building up and its application of the siRNA for the machine learning model of the toxicity of certain class cell |
| CN108664769A (en) * | 2017-03-31 | 2018-10-16 | 中国科学院上海生命科学研究院 | Large-scale medicine method for relocating based on cancer gene group and non-specific gene label |
-
2000
- 2000-11-06 CN CN 00120549 patent/CN1352306A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101784895A (en) * | 2007-06-26 | 2010-07-21 | 协乐民公司 | Method for predicting biological systems responses in hepatocytes |
| CN108182346A (en) * | 2016-12-08 | 2018-06-19 | 杭州康万达医药科技有限公司 | Predict method for building up and its application of the siRNA for the machine learning model of the toxicity of certain class cell |
| CN108182346B (en) * | 2016-12-08 | 2021-07-30 | 杭州康万达医药科技有限公司 | Establishment method and application of machine learning model for predicting toxicity of siRNA to certain cells |
| CN108664769A (en) * | 2017-03-31 | 2018-10-16 | 中国科学院上海生命科学研究院 | Large-scale medicine method for relocating based on cancer gene group and non-specific gene label |
| CN108664769B (en) * | 2017-03-31 | 2021-09-21 | 中国科学院上海营养与健康研究所 | Drug relocation method based on cancer genome and non-specific gene tag |
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Addressee: Fama Gene Science and Technology Co., Ltd., Chengdu Document name: Deemed as a notice of withdrawal |
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