CN1346892A - Biochip for joint detection of gastrosis - Google Patents
Biochip for joint detection of gastrosis Download PDFInfo
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- CN1346892A CN1346892A CN 01126896 CN01126896A CN1346892A CN 1346892 A CN1346892 A CN 1346892A CN 01126896 CN01126896 CN 01126896 CN 01126896 A CN01126896 A CN 01126896A CN 1346892 A CN1346892 A CN 1346892A
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Abstract
A biochip for detecting pylorospirobacillus, its toxin factor and the gastric ulcer and cancer caused by it is disclosed. The biochip kit is composed of carrier box and protein test paper. Said carrier box has at least 5 carrier slots for recepting different test paper to detect Hp-IgG, Hp-IgM, CagA-HpIgG, VacA-HpIgG and pepsinogen-I.
Description
The present invention relates to a kind of stomach trouble joint-detection diagnostic device, particularly relate to the biochip of a kind of parallel joint-detection helicobacter pylori, Peptic Ulcers and cancer of the stomach differential diagnosis.
Helicobacter pylori (Helicobacter, Hp) at the infection rate of China's population up to 50-80%, foreign study finds have the Hp bacterial strain of cytotoxic associated protein (CagA) to be high strain, and it is the important paathogenic factor of chronic active gastritis, peptide ulceration.Numerous retrospective and prospective epizootic disease researchs show that also Hp still causes the important virulence factor of a class of cancer of the stomach.Can make that stomach reactivity inflammation disappears, ulcer healing and eradicate Hp, and significantly reduce the ulcer recurrence rate, make malignant lymphoma of stomach obviously dwindle or disappears, therefore convenient, effectively detect and prevent extremely arduousness of task that Hp infects.
Often adopt radioimmunoassay (RIA) or enzyme linked immunosorbent assay analysis method (ELISA) clinically, by detecting anti-Hp-IgG in the serum, anti-Hp-IgM is with the infection conditions of diagnosis HP, and with CagA-HpIgG, VacA-HpIgG is as the index of diagnosis Hp virulence factor, peptic ulcer disease patients serum propepsin-I obviously raise (existence of ulcer is described) is also found in research recently, and reduce all the time in the level of patients with gastric cancer Serum Pepsinogen-I, so propepsin-I can be used as the diagnosis index of differentiating Peptic Ulcers and cancer of the stomach, two kinds of methods have sensitivity preferably, specificity and stability, but exist inspection comparatively loaded down with trivial details, last longer, unhandy deficiency.Simultaneously, detect single albumen test strip of HP antibody because it is easy and simple to handle, reaction fast, and have characteristics such as certain specificity, stability and sensitivity, the analysis and the detection of the various samples in medical diagnosis field have been applied to, but also can only analyze a kind of detection index, the amount of diagnostic information that is provided is limited and not comprehensive at every turn.
The objective of the invention is to provide a kind of biochip that quick and precisely detects helicobacter pylori and virulence factor, Peptic Ulcers and cancer of the stomach differential diagnosis, by parallel joint-detection, can not only make diagnosis to infection conditions and the virulence factor of helicobacter pylori, realize early discovery, early diagnosis, early treatment, and to the early diagnosis and the discriminating of closely-related Peptic Ulcers and cancer of the stomach all have significant values with it.
For achieving the above object, the present invention has adopted following technical scheme: a kind of rapid detection helicobacter pylori, the biochip of Peptic Ulcers and cancer of the stomach differential diagnosis, it is made up of carrier box and protein test strip, described carrier box contains carrier box panel and carrier box backboard, be provided with the carrier channels that to place the test strip that (contains five) more than five in the described carrier box backboard, described test strip is by the suction zones of upper end, the observation district of middle-end and the sample introduction district of lower end constitute, described carrier box panel bottom is an injection port, the middle part is the control line-transect between porthole and injection port and the porthole, carrier channels more than five is arranged in the described carrier box backboard, place anti-Hp-IgG and the anti-Hp-IgM that is used to detect helicobacter pylori infection in the carrier channels respectively, the CagA-HpIgG of diagnosis Hp virulence factor, the test strip of five indexs such as propepsin-I of VacA-HpIgG and discriminating Peptic Ulcers and cancer of the stomach, utilize chip technology integrated, high-throughput, the principle of parallelization, with these protein test paper with parallel or fan-shaped mode arbitrary combination, integrate, form biochip for joint detection of gastrosis, these five kinds of indexs in the disposable detection testing sample of its energy.
The present invention has not only improved the operation inconvenience of radioactive immunoassay (RIA) and enzyme-linked immunosorbent assay method (ELISA), time-consuming, deficiency such as cost an arm and a leg, and than the abundant information that obtains from single HP antibody protein test strip and comprehensively.By disposable joint-detection, not only can make correct judgement to infection and the virulence factor thereof of helicobacter pylori fast, can also realize to the diagnosis and the discriminating of closely-related Peptic Ulcers of helicobacter pylori infection and cancer of the stomach.Therefore all has very high use value at aspects such as the clinical detection of general health self check, hospital and diagnosis.
Below in conjunction with accompanying drawing the embodiment of the invention is done further statement.
Fig. 1 shows biochip for joint detection of gastrosis carrier box base synoptic diagram.
Fig. 2 shows biochip for joint detection of gastrosis carrier box synoptic diagram.
Fig. 3 shows protein test strip synoptic diagram.
With reference to Fig. 1, Fig. 2 and Fig. 3, rapid detection helicobacter pylori of the present invention, the biochip of Peptic Ulcers and cancer of the stomach differential diagnosis is made up of carrier box (1) and protein test strip (8), described carrier box (1) contains carrier box panel (2) and carrier box backboard (3), described carrier box backboard (3) is fan-shaped, be provided with the carrier channels (4) that to place five protein test strip in the carrier box, described test strip has the suction zones (11) of upper end, the observation district (10) of middle-end and the sample introduction district (9) of lower end constitute, described panel bottom is injection port (5), the middle part is the control line-transect (7) between porthole (6) and injection port (5) and the porthole (6), places respectively in the carrier channels and can be used in anti-Hp-IgG and the anti-Hp-IgM that detects helicobacter pylori infection, the CagA-HpIgG of diagnosis Hp virulence factor, the test strip (8) of five indexs such as propepsin-I of VacA-HpIgG and discriminating Peptic Ulcers and cancer of the stomach.Used protein test strip also can add other protein-specific antibody on the basis that contains above five kinds of diagnosis indexs, form the stomach trouble joint-detection chip that contains five above protein test strip.
During use, when adding the sample that to detect from injection port (5), the detection index that is wherein contained can combine with the golden labeling antibody in the sample introduction district (9) on the protein test strip of this index of biochip relevant detection, distinguish (10) along the membrane chromatographic of each test paper to observing respectively, and with detection line on the bag quilt specific antibody combine, in porthole (6), form macroscopic red stripes, excessive mixture or unconjugated gold mark thing can continue chromatography, the macroscopic red lines of formation combine with the antibody of control line (quality control line), article two, red lines then show the positive, if have only the quality control line to form red lines then show feminine gender, if detection line and quality line all not colour developing show invalid.So just can realize the differential diagnosis of disposable parallel detection helicobacter pylori and virulence factor, Peptic Ulcers and cancer of the stomach.
Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example, usually according to the condition described in normal condition such as the laboratory manual, or the condition of advising according to manufacturer.
Claims (2)
1. parallel joint-detection helicobacter pylori, the biochip of Peptic Ulcers and cancer of the stomach differential diagnosis, it is made up of carrier box (1) and protein test strip (8), described carrier box (1) contains carrier box panel (2), carrier box backboard (3), the middle part of described carrier box panel (2) is provided with porthole (6), the bottom is provided with injection port (5), be provided with control line-transect (7) between porthole and the injection port, the upper end of protein test strip is suction zones (11), middle-end is for observing district (10), the lower end is sample introduction district (9), it is characterized in that described carrier box backboard has the carrier channels that (comprises five) more than five and deposit the protein test strip, lays at least in the described carrier channels and can detect anti-Hp-IgG, Hp-IgM, CagA-HpIgG, VacA-HpIgG, the protein test strip of five kinds of indexs of propepsin-I.
2. according to the biochip of the described parallel joint-detection helicobacter pylori of claim 1, Peptic Ulcers and cancer of the stomach differential diagnosis, it is characterized in that in the carrier channels in the described carrier box backboard protein test strip of can also addition making with other protein-specific antibody.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01126896 CN1346892A (en) | 2001-09-28 | 2001-09-28 | Biochip for joint detection of gastrosis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01126896 CN1346892A (en) | 2001-09-28 | 2001-09-28 | Biochip for joint detection of gastrosis |
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| CN1346892A true CN1346892A (en) | 2002-05-01 |
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| CN 01126896 Pending CN1346892A (en) | 2001-09-28 | 2001-09-28 | Biochip for joint detection of gastrosis |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104142399A (en) * | 2013-05-08 | 2014-11-12 | 北京美康生物技术研究中心有限责任公司 | Test paper strip using colloidal gold immunochromatographic technology for quantitative determination of serum pepsinogen and preparation method and application thereof |
| CN104422772A (en) * | 2013-09-10 | 2015-03-18 | 江苏省原子医学研究所 | Time resolution immunochromatographic test strip for quantitatively detecting pepsinogen I as well as preparation method of time resolution immunochromatographic test strip |
| CN104422775A (en) * | 2013-08-22 | 2015-03-18 | 朱建安 | Fluorescent immunochromatography test paper for combined detection of human PGI protein and human PGII protein, and production method |
| CN104422773A (en) * | 2013-08-22 | 2015-03-18 | 朱建安 | Immunochromatography test paper for detecting human PGI protein and preparation method thereof |
| CN104422774A (en) * | 2013-08-22 | 2015-03-18 | 朱建安 | Immunochromatography test paper for detecting human PGII protein and preparation method thereof |
| CN108061807A (en) * | 2018-02-02 | 2018-05-22 | 江苏出入境检验检疫局动植物与食品检测中心 | It is a kind of to be used to measure immunofluorescent reagent item of CagA+ Hp antibody and its preparation method and application |
| CN109001461A (en) * | 2018-06-26 | 2018-12-14 | 安阳工学院 | The immune quick testing reagent box of PCV-3 immunity evaluation and wild virus infection diagnosis |
| CN111192653A (en) * | 2020-01-20 | 2020-05-22 | 张学堂 | Family helicobacter pylori decision self-guiding system |
-
2001
- 2001-09-28 CN CN 01126896 patent/CN1346892A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104142399B (en) * | 2013-05-08 | 2016-03-02 | 北京美康生物技术研究中心有限责任公司 | A kind ofly utilize colloidal gold immunochromatographimethod technology quantitatively test strips detecting Serum Pepsinogen and its preparation method and application |
| CN104142399A (en) * | 2013-05-08 | 2014-11-12 | 北京美康生物技术研究中心有限责任公司 | Test paper strip using colloidal gold immunochromatographic technology for quantitative determination of serum pepsinogen and preparation method and application thereof |
| CN104422775B (en) * | 2013-08-22 | 2016-05-18 | 朱建安 | Fluorescence immune chromatography test paper and the method for making of joint-detection people PGI albumen and people PGII albumen |
| CN104422773A (en) * | 2013-08-22 | 2015-03-18 | 朱建安 | Immunochromatography test paper for detecting human PGI protein and preparation method thereof |
| CN104422774A (en) * | 2013-08-22 | 2015-03-18 | 朱建安 | Immunochromatography test paper for detecting human PGII protein and preparation method thereof |
| CN104422775A (en) * | 2013-08-22 | 2015-03-18 | 朱建安 | Fluorescent immunochromatography test paper for combined detection of human PGI protein and human PGII protein, and production method |
| CN104422774B (en) * | 2013-08-22 | 2016-07-06 | 朱建安 | Fluorescence immune chromatography test paper of detection people's PGII albumen and preparation method thereof |
| CN104422773B (en) * | 2013-08-22 | 2016-08-24 | 朱建安 | Fluorescence immune chromatography test paper of detection people's PGI albumen and preparation method thereof |
| CN104422772A (en) * | 2013-09-10 | 2015-03-18 | 江苏省原子医学研究所 | Time resolution immunochromatographic test strip for quantitatively detecting pepsinogen I as well as preparation method of time resolution immunochromatographic test strip |
| CN108061807A (en) * | 2018-02-02 | 2018-05-22 | 江苏出入境检验检疫局动植物与食品检测中心 | It is a kind of to be used to measure immunofluorescent reagent item of CagA+ Hp antibody and its preparation method and application |
| CN109001461A (en) * | 2018-06-26 | 2018-12-14 | 安阳工学院 | The immune quick testing reagent box of PCV-3 immunity evaluation and wild virus infection diagnosis |
| CN109001461B (en) * | 2018-06-26 | 2024-02-02 | 安阳工学院 | Immune rapid test kit for PCV-3 immune evaluation and wild virus infection diagnosis |
| CN111192653A (en) * | 2020-01-20 | 2020-05-22 | 张学堂 | Family helicobacter pylori decision self-guiding system |
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