CN1346058A - Apparatus for automatic treating liquid sample - Google Patents
Apparatus for automatic treating liquid sample Download PDFInfo
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- CN1346058A CN1346058A CN 01133935 CN01133935A CN1346058A CN 1346058 A CN1346058 A CN 1346058A CN 01133935 CN01133935 CN 01133935 CN 01133935 A CN01133935 A CN 01133935A CN 1346058 A CN1346058 A CN 1346058A
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Abstract
Provided is a system for treating samples, comprising a programmable controller working with a sampling mode and at least one cleaning mode. In the sampling mode, samples with predetermined volume are sucked into an optical article carrier assembly, and in the cleaning mode, cleaning fluid with predetermined volume is transferred to remove samples out of the carrier assembly. The programmable controller supervises the liquid pressure of each sampling and cleaning mode, and controllably delays response to trigger of an alarm system for an unacceptable high pressure detected after a pump assembly stops. The system has an optical indicating assembly, which changes its color controllably and gradually to indicate the performance level of each sampling and cleaning mode.
Description
Technical field
The present invention relates to a kind of method and apparatus that is used for automatic treating liquid sample.Especially, the present invention relates to a kind of Programmable Logic Controller, it carries out liquid sample successively and is sent to optics loading assembly, and sample is discharged from it, and cleans the liquid supplying part of this device.The invention still further relates to a kind of method that this device partly stops up with the FLUID TRANSPORTATION of timely sniffer that is used for controllably handling.
Background technology
The method that is used for the device of fluid analysis and is used to handle these devices in the clinic, laboratory and hospital be extensive use of.General these assemblies need efficient enough high with in the short relatively time and so that user and want directly to contact minimum secured fashion between the detected sample and carry out big relatively quantitative analysis.
Simple and the efficient system that is used to carry out urinalysis is at the United States Patent (USP) 5,393,494 and 5,895 of authorizing people such as Greenfield, and is open in 762.It has been described and has been used for the liquid sample suction is used for apparatus and method by fractographic loading assembly (slide assembly).This device comprises the hard-wired flogic system that allows device to carry out sequence of operations under the mode of monitoring.
This system has been proved effectively and is reliable.Yet because the limitation of hard-wired logic inherence, this system is not very flexible.Thereby the operation of liquid feeding assembly is only controlled directly with the reference value deposited and variable element comparison the time.For example, if the solid impurity that liquid transfer partly is present in the liquid sample blocks, in case pressure reaches predetermined value, the operation cycle of total system is interrupted.Yet frequent is that it not is the temporary transient peak value of expression system actual pressure that the fluctuation of pressure may have.Yet warning system is unlocked immediately, causes the operator to interfere after reaching predetermined value.
Equally, as mentioned above, suitable vision and audible indication that some known systems do not provide each device to handle performance level are no matter be cleaning model or decimation pattern.As a result, the operator is the operation of interference system unintentionally, and causes unnecessary the stopping of system at least.
Therefore, needed is a kind of device that is used for the fluid transfer sample, and it has the Programmable Logic Controller of the sequence of operation of supervising device.The device that is used for the fluid transfer sample has supervisory system, and it can minimize the appearance that needs the false alarm that the operator interferes.What need equally is a kind of device that is used for the fluid transfer sample, and it has supervisory system, and this system provides the vision that the increases progressively indication of workstation operation, and the roughly indication of remaining time that is provided for complete operation.
Summary of the invention
So the device that is used for automatic treating liquid sample of the present invention provides a series of programmable operations, according to different liquids transmission in device of its predetermined.
The inventive system comprises Programmable Logic Controller, it has reduced the risk of opening false alarm, even reached the reference value that is considered to threshold value at the pressure of system.This is by storage pressure limit up and down, and decline period of providing the pressure of system to allow to descend in tolerance interval realizes.If continue high or low pressure in the period in predetermined down after just cleaning or decimation pattern are finished, alarm is just opened, and requires the user to interfere.Thereby decline period of being used to trigger warning system has reduced mainly the generation that the liquid translator unit that is illustrated in device is taken place for the counteractive rub-out signal of lasting situation of blocking.
According to a further aspect of the invention, device of the present invention has the indication mechanism of counting down, and it helps the user clearly to see its time that must wait for before the operation of pattern is finished at present.This is along with the operation of present pattern is finished progress and changed its state successively and realize to it by a series of optical indicators such as LED.Each LED that extinguishes represents the performance level of this pattern, and provides the vision indication of what being finished and what also remained before whole pattern being finished to the user.
In a word, device according to the present invention provides a series of operations of monitoring and indication, and it prevents that the user from before device is cleaned and prepares to carry out another test, testing new liquid sample.
Description of drawings
On address other purpose, feature and advantage and will from the description of the illustrative embodiment of the present invention shown in the following drawings, become clearer, wherein
Fig. 1 is the overall calcspar that is used in the device that is used for automatic treating liquid sample in the liquid sample analysis according to the present invention;
Fig. 2 is the process flow diagram that the whole operation order of device is shown;
Fig. 3 is the process flow diagram that the sequence of operation that constitutes initialize mode is shown;
Fig. 4 is the process flow diagram that the sequence of operation that constitutes decimation pattern is shown;
Fig. 5 is the curve signal of explanation decimation pattern;
Fig. 6 is the process flow diagram that the sequence of operation that constitutes cleaning model is shown;
Fig. 7 is the curve signal of cleaning model;
Fig. 8 is the process flow diagram that the sequence of operation that constitutes the expansion cleaning model is shown;
Fig. 9 is the curve signal of expansion cleaning model.
Embodiment
With reference to Fig. 1-9, a device 10 is shown, by it liquid sample is taken out in holding container 12 via extracting assembly 14, and deliver to loading assembly 16 with at least one observation ward 18.The loading assembly is flat structure, its have with above-mentioned United States Patent (USP) 5393494 in specifically described consistent total shape.Therefore, this patent is herein incorporated as reference, and it is made improvement as described herein.
The loading assembly is installed on the standard microscope 20 and two mouths 22 and 24 that provide near observation ward is provided, and this observation ward is provided with elongated clear glass seamless box again.Mouthfuls 22 are connected to by flexible pipe 26 and extract on the assembly 14, and mouthfuls 24 be communicated with bleaching cistern 28 and washing fluid groove 30 fluids that preferably comprise salt solusion by electromagnetism controlled valve 32 and 34 respectively.Valve 32 is used to select or bleach circuit 29 or the circuit 31 that leads to the loading assembly.Valve 34 is safety valve, when it is closed, stops up the circuit that leads to the loading assembly, makes the residual pressure release that may residue in the helicoidal pump (peristaltic pump) 40 simultaneously, as will be described below.
The pump assembly that comprises stepper motor 38 and reciprocal helicoidal pump 40 is communicated with the loading assembly and with groove 28 and 30 fluids by each valve.Finally, the pressure transducer 42 that is suitable for pressure in the measuring system is connected on the inlet/outlet of pump 40.
Programmable Logic Controller 44 is used in the operation of system 10, it comprise the system pressure that is used for (storage) such as benchmark or expectation and pump 40 stop after the storer and the microprocessor of the desired fall off rate of this pressure.Usually, controller 44 supervisory systems 10 selectively basic, extract, clean and the expansion cleaning model under the operation river pagination followed.
Specifically, controller 44 is handled the valve-driving circuit 46 that is connected in monitor valve 32 and 34, by the pressure monitor circuit 48 sensing overpressure conditions of surveying by pressure transducer 42, and the stepper motor driving circuit 50 of monitoring drive stepping motor 38.
The starting of system mainly begins to move down normal wash switch 56 and power-on switch 52, because device is assumed that previous no initializtion, this is attended by LED54 and lights.As a result, controller 44 is powered in 66 steps (Fig. 2), and at first device is converted to initialize mode 58 (Fig. 2 and 3), and its purpose is for sending into salt solusion in all fluid conduction routes that extract between assembly 14 and the pump 40.
Especially, according to stored program, valve 32 and 34 is round-robin, and stepper motor 38 is in step 68 (Fig. 3) actuated pump 40, salt solusion being brought in pump, the pressure transducer 42, and through each valve, enter the loading assembly 70, and salt solusion is removed from this assembly.Equally, by washing away direction D rotation pump, salt solusion is admitted to the circuit 29 that leads to bleaching cistern 28, and in addition, is sample direction S by changing turning to of pump, and bleaching agent is extracted finally to discharge system in step 72 with salt from groove 28.At this moment, system is cleaned and prepares to be used to accept sample with in 74 times tests of decimation pattern, and this is provided with automatically by controller, even under the situation that device has been closed and opened after carrying out initialize mode.
The LED76 of sampling and normal mode lights, and indication mechanism is initialised.
In order to operate a sample, extract assembly 14 and be placed in the holding container 12, and when pressing sampling switch 80, sample takes out from extract assembly and enters observation ward 18.For fear of this transmission, valve 34 triggers in step 82 (Fig. 4), and pump 40 is in sample direction S running, to extract assembly 14 downstreams generation vacuum.According to program stored in processor, stepper motor is in the step number rotation of step 84 with some, and per step makes rotor rotate a uniform angle, for example 1.8 °.In case motor moved the step number that representative is drawn into the desirable sample size in the observation ward, the device shut-down operation, this extinguishes expression by sampling switch LED80.Device is programmed and makes that new sample can not be tested, removes nonsystematic and is cleaned, and this is represented by the cleaning LED86 that lights.
The feature of the beginning of decimation pattern is that also a plurality of LED90 are recycled clock driver circuit 92 power supplies, provide counting down of this operation to proceed to its end along with extraction operation.Finishing of an extraction operation part represented in extinguishing of each LED90 in turn.Thereby the user who watches these LED attentively can recognize the time that must wait for before whole pattern finishes.In case motor stops in step 96 (Fig. 4), last LED90 extinguishes in step 96.As a result, prevent from further to extract sample out, although valve 34 remains unlatching from decimation pattern.
Internal pressure is detected the predetermined reference value P that is lower than in the storer that is stored in controller 44 if the pump assembly stops the back system in order to prevent
Ref, producing a false alarm, the software of carrying out on controller provides a controlled delay period.As a result, under-voltage LED98 of system and audio alarm 100 can not activated, and remove non-pressure and are postponing period normalization not yet when finishing.
This is shown among Fig. 5 in proper order, has drawn the system pressure P of relative time T among the figure.Specifically, along with the pump assembly rotates predetermined step number producing negative pressure in sample direction, the sample of definite volume be sent in the loading assembly.In case at t
1Motor stops constantly, in order to determine whether system occurs blocking, and represents measured pressure P
M1Signal be converted into digital form and controlled device reads.If P
M1Less than reference value P
Ref, controller will be waited for the signal P that sends in such as 1/2 second predetermined time interval
M2If measure and point out system still under-voltage next time, another period t will programme
2-t
1, with the triggering of further delayed alarm system.Only when postponing under-voltage situation that the period represents that system takes place to block when finishing and still keep, warning system triggers.
Owing to, replace directly with reference value P by the dirigibility that Programmable Logic Controller provided
RefCompare with the system pressure that records, and supervisory system rate of pressure change dp/dt.If be slower than desired value in delay this speed in latter stage period, warning system is closed.
If at period t
1Back system pressure P
M1Recorded and be higher than datum pressure P
Ref, sampling LED opens again, and valve 34 is de-energized, and the operation of normal wash next time or expansion cleaning model is prepared by system.
With reference to Fig. 6 and 7, the normal wash pattern is programmed to similar to the sequence of operation of decimation pattern, except as shown in Figure 7.The liquid system pressure P that hope is produced by salt solusion
mRaise, so that driving is from the liquid sample of loading assembly.Specifically, in case normal wash mode switch 86 is pressed, and controller scans it, and valve 34 is in step 102 power supply, provides to the fluid path of loading assembly to clean being used for.Stepper motor rotates predetermined step number to discharge the salt solusion that remains to be discharged of predetermined from the salt solusion groove in step 104 to wash away direction.Stepper motor step 108 stop and circulation clock LED90 after step 106 is extinguished, system prepare to detect is stopped up.At the fixed time the section in measuring system pressure, and if its remain on expectation value or reference value P
RefOn, warning system is closed.Similar with decimation pattern, might supervisory system pressure fall off rate dp/dt, thus the closing of control electromagnetic valve.If under being longer than home its should time period of length in, the liquid system pressure P
mRemain unchanged or descend, obstruction has appearred in system so.
By moving down expansion cleaning model button 110 and moving down the normal wash mode switch, the user can be converted to mode of extension with system.Be different with normal rinse mode of mode of extension transmitted salt solusion with faster rate.In addition, when mode of extension began, valve 32 was powered bleaching agent being sent in the system between valve 32 and 34 in step 112, and further discharges from system.Similar with normal rinse mode, obstruction is to determine by the system pressure and/or the system pressure fall off rate of monitoring after the flushing liquid of volume of ideal is transmitted.
Although the present invention is described with reference to concrete arrangement of parts, feature etc., these also are not used in and comprise all possible layout or feature, and multiple for those skilled in the art modification and change all can be conceived really.
Claims (20)
1. device that is used to transmit the liquid suspension of certain volume comprises:
The sample storage assembly for storing that comprises the liquid sample of wanting tested;
With the optics loading assembly of sample storage assembly for storing fluid connection, to accept to be used for liquid sample by microscope direct observing;
The cleaning liquid reservoir that is used to deposit cleaning liquid and flows and be communicated with optics loading assembly;
In relative sampling with wash away the rotating pump assembly of direction;
Programmable Logic Controller;
The software of on Programmable Logic Controller, carrying out, be used for the pump assembly is changed between decimation pattern and cleaning model, in decimation pattern, the pump assembly rotates to extract the sample liquid of predetermined from the sample storage assembly for storing in sample direction, and in cleaning model, the pump assembly rotates with the flushing liquid that drives predetermined by optics loading assembly with from wherein removing sample washing away direction; And
By the color coding assembly of Controlled by Programmable Controller, with from a state to the order of another state according to pre-programmed, response is extracted and is cleaned finishing gradually of each pattern and change its color gradually.
2. device as claimed in claim 1, wherein, the color coding assembly comprise the circulation clock circuit and by the circulation clock driver circuit along with each pattern row's circulation clock LED of outage to the progress of finishing and in turn so that each in turn each pattern of expression of extinguishing of optical indicator finish last approximate time.
3. device as claimed in claim 1, wherein, the pump assembly comprises stepper motor and helicoidal pump, the step number rotation that stepper motor can be scheduled to is to extract the liquid sample and the cleaning liquid of pre-programmed volume in corresponding pattern.
4. device as claimed in claim 1 also comprises at least one first controlled valve between flushing liquid reservoir and optics loading assembly, described first controlled valve is energized the liquid that flows to the loading assembly to provide.
5. device as claimed in claim 1 also is included in the software of carrying out on the Programmable Logic Controller, is used for it is transformed into the expansion cleaning model.
6. device as claimed in claim 5 also comprises the bleaching agent accumulator tank and second controlled valve that have bleaching agent, and the liquid transmission route that described second controlled valve control bleaching agent flows between loading assembly and the cleaning liquid reservoir reaches from its outflow.
7. device as claimed in claim 5, wherein, second valve is powered with when controllably rotating the pump assembly according to the order of pre-programmed in mode of extension, permission is extracted bleaching agent from the bleaching agent accumulator tank, and the pump assembly at first rotates in sample direction, and is washing away the direction rotation so that bleaching agent is discharged by the loading assembly then.
8. device as claimed in claim 1 also is included in the pressure detector of measuring fluid pressure in each pattern, and is reaching the alarm assemblies that predetermined threshold value pressure makes generation sound and visual signal.
9. device as claimed in claim 8, also be included in the software of carrying out on the Programmable Logic Controller, the triggering of delayed alarm assembly controllably when being used for reaching threshold pressure after the rotation of stepper motor is stopped, described fluid pressure is postponing compartment of terrain measurement in the period according to the order of pre-programmed.
10. device as claimed in claim 9, also be included in the software of carrying out on the Programmable Logic Controller, be used for determining to postpone the speed that fluid pressure descends in the period and be used to monitor this speed, so that alarm assemblies is only postponing that the period finishes if pressure descends to be opened when being slower than threshold value, described first valve cuts off the power supply when finishing postponing the period.
11. a device that is used to transmit the liquid suspension of certain volume comprises:
Comprise the optics loading assembly of observation ward, be used to control the liquid sample that is used for by microscope direct observing;
The sample storage assembly for storing that comprises sample liquid and flow and be communicated with observation ward;
The cleaning liquid reservoir that is used to deposit cleaning liquid and flows and be communicated with observation ward;
Sample direction opposite with sample direction wash away the rotating pump assembly of direction;
Pressure transducer between loading assembly and pump assembly is used for the fluid pressure in the sniffer and produces the signal of echo probe;
Programmable Logic Controller;
The software of on controller, carrying out, be used for the pump assembly is changed between decimation pattern and cleaning model, in decimation pattern, the pump assembly rotates with the sample liquid suction optics loading assembly with predetermined in sample direction, and in cleaning model, the flushing liquid of predetermined passes through observation ward from the cleaning liquid reservoir, so that sample is removed from optics loading assembly;
The software of carrying out on controller is used for controllably interrupting the rotation of pump assembly after the sample of each described extraction and cleaning model predetermined and cleaning liquid are transmitted respectively; And
The software of carrying out on described Programmable Logic Controller is used for having stopped back monitoring fluid pressure in compartment of terrain in the preset time section at pump.
12. device as claimed in claim 11, also comprise the alert component that comprises sound and visual signal, after stepper motor stops, fluid pressure is surveyed with being spaced apart and is compared with reference value, with in scheduled time slot, if fluid pressure surpasses reference value, make described alarm assemblies can not produce sound and visual signal.
13. device as claimed in claim 12, wherein, at least 1/4 second compartment of terrain was surveyed three times between fluid pressure was surveyed with each time.
14. device as claimed in claim 13, wherein, described interval is uniform.
15. device as claimed in claim 13, wherein, described interval is heterogeneous.
16. device as claimed in claim 13, wherein, the software that is used for monitoring fluid pressure provides condition for monitoring at the fall off rate of each described extraction and cleaning model fluid pressure.
17. device as claimed in claim 11 also comprises first controlled valve that is inserted between pump assembly and the optics loading assembly, described first controlled valve is optionally powered with the liquid in the control inflow optics loading assembly.
18. device as claimed in claim 17, also comprise the bleaching agent memory module and prevent that in cleaning model bleaching agent from entering second controlled valve of optics loading assembly, be powered so that flowing between bleaching agent memory module and the optics loading assembly to be provided at second controlled valve described in the expansion cleaning model.
19. device as claimed in claim 12 also comprises software, it is used to prevent that decimation pattern from beginning to test new liquid sample, unless cleaning model has formerly been carried out.
20. one kind is used for the certain volume liquid suspension being conveyed into the loading assembly and being used for its device from that discharge is comprised;
Selectively in relative sampling with wash away the pump assembly that direction is rotated;
With the pressure transducer that the pump assembly flows and is communicated with, be used for the pressure of liquid in the sniffer;
The Programmable Logic Controller of between decimation pattern and at least one cleaning model, changing, in decimation pattern, the pump assembly rotates with the liquid sample suction loading assembly with predetermined in sample direction, and in described at least one cleaning model, the cleaning liquid of predetermined is driven through the loading assembly so that liquid sample is removed from the loading assembly; And
The software of carrying out on Programmable Logic Controller is used for extracting and one of cleaning model is determined the fall off rate of the fluid pressure after the pump assembly stops in the mode of time control.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US66798600A | 2000-09-22 | 2000-09-22 | |
| US09/667,986 | 2000-09-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1346058A true CN1346058A (en) | 2002-04-24 |
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ID=24680501
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01133935 Pending CN1346058A (en) | 2000-09-22 | 2001-08-16 | Apparatus for automatic treating liquid sample |
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| Country | Link |
|---|---|
| CN (1) | CN1346058A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101292186B (en) * | 2005-10-21 | 2010-06-09 | 株式会社尼康 | Liquid immersion microscope |
| CN103323611A (en) * | 2010-04-01 | 2013-09-25 | 株式会社东芝 | Automatic analyzer |
| CN103648649A (en) * | 2011-05-18 | 2014-03-19 | 3M创新有限公司 | Systems and methods for detecting the presence of a selected volume of material in a sample processing device |
| WO2020020169A1 (en) * | 2018-07-23 | 2020-01-30 | 深圳市帝迈生物技术有限公司 | Sample analyzing device and cleaning method thereof |
-
2001
- 2001-08-16 CN CN 01133935 patent/CN1346058A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101292186B (en) * | 2005-10-21 | 2010-06-09 | 株式会社尼康 | Liquid immersion microscope |
| CN103323611A (en) * | 2010-04-01 | 2013-09-25 | 株式会社东芝 | Automatic analyzer |
| US8999267B2 (en) | 2010-04-01 | 2015-04-07 | Kabushiki Kaisha Toshiba | Automatic analyzer |
| US9606134B2 (en) | 2010-04-01 | 2017-03-28 | Toshiba Medical Systems Corporation | Automatic analyzer |
| CN103648649A (en) * | 2011-05-18 | 2014-03-19 | 3M创新有限公司 | Systems and methods for detecting the presence of a selected volume of material in a sample processing device |
| US9168523B2 (en) | 2011-05-18 | 2015-10-27 | 3M Innovative Properties Company | Systems and methods for detecting the presence of a selected volume of material in a sample processing device |
| CN105170203B (en) * | 2011-05-18 | 2017-07-18 | 3M创新有限公司 | Material system and method present in sample processing apparatus of the selected volume of detection |
| US9725762B2 (en) | 2011-05-18 | 2017-08-08 | Diasorin S.P.A. | Systems and methods for detecting the presence of a selected volume of material in a sample processing device |
| WO2020020169A1 (en) * | 2018-07-23 | 2020-01-30 | 深圳市帝迈生物技术有限公司 | Sample analyzing device and cleaning method thereof |
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