CN1341587A - Method for preparing resveratrol from giant knotweed rhizome - Google Patents
Method for preparing resveratrol from giant knotweed rhizome Download PDFInfo
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- CN1341587A CN1341587A CN 01126769 CN01126769A CN1341587A CN 1341587 A CN1341587 A CN 1341587A CN 01126769 CN01126769 CN 01126769 CN 01126769 A CN01126769 A CN 01126769A CN 1341587 A CN1341587 A CN 1341587A
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- resveratrol
- giant knotweed
- ethyl acetate
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- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The method for preparing resveratrol by using bushy knotweed root includes the following steps: using fresh bushy knotweed root and pulping, placing the obtained pulp material in a container, storing for 24-120 hr. at 10-50 deg.C, and stirring, then using ethyl acetate to make extraction, recovering ethyl acetate, further making purification treatment so as to obtain resveratrol. Because the resveratrol glycoside being in the bushy knotweed root can be converted into resveratrol under the condition without foreign enzyme added. so it has the advantages of simple operation, high yield and low cost.
Description
Technical field
The present invention relates to a kind of from giant knotweed the method for extraction separation trans-resveratrol.
Background technology
Contain trans-resveratrol (resveratrol) and glycoside thereof in the giant knotweed, trans-resveratrol has reducing blood-fat, anti-oxidant, microcirculation improvement, hypotensive and suppress effects such as staphylococcus, suis.
The method of extracting trans-resveratrol mainly contains two classes, and a class at first adopts the interpolation prozyme that the glycoside of trans-resveratrol is carried out enzymolysis, and with solvent extraction, separation and purifying, preparation cost is higher again.Another kind ofly directly extract with solvent, with component separating such as trans-resveratrol glycoside, product yield is low.
Summary of the invention
Purpose of the present invention is exactly to provide the method for preparing trans-resveratrol from giant knotweed that a kind of technology is simple, cost is low, recovery rate is high for the defective that overcomes above-mentioned prior art existence.
Purpose of the present invention can be achieved through the following technical solutions: a kind of method for preparing trans-resveratrol from giant knotweed, it is characterized in that, comprise following processing step, at first, place container fresh giant knotweed making beating, placed 24~120 hours down at 10~50 ℃, and stir, use ethyl acetate extraction then, reclaim ethyl acetate, through being further purified processing, make trans-resveratrol again.
Adopt identical giant knotweed to compare test with the present invention and existing direct extraction method respectively, test-results shows that the recovery rate of the trans-resveratrol that the present invention makes can improve more than 2 times than direct extraction method.The present invention since giant knotweed in the trans-resveratrol glycoside under the situation that need not add exogenous enzyme, be converted into trans-resveratrol, thereby have simple to operate, recovery rate is high, low cost and other advantages.
Description of drawings
Fig. 1 is a process flow sheet of the present invention.
The specific embodiment
Tie below specific embodiment and accompanying drawing, the invention will be further described.
Embodiment 1
Get bright giant knotweed I, to contain resveratrol (not comprising glycoside) be 0.16g to 100g after measured, takes by weighing 500g, Pulverize pulping, 30 ± 2 ℃ of lower placements 96 hours, per hour stirred in the put procedure 5 minutes, use acetic acid Solvent is reclaimed in the ethyl ester extraction, and more purified, getting white crystals is resveratrol 1.89g. If should tiger The cane slurry is directly used ethyl acetate extraction, by the same procedure purifying, gets resveratrol 0.61g.
Embodiment 2
Get bright giant knotweed I, to contain resveratrol (not comprising glycoside) be 0.16g to 100g after measured, takes by weighing 500g, Pulverize pulping, 40 ± 2 ℃ of lower placements 72 hours, stirred 5 minutes in per 2 hours in the put procedure, use second Solvent is reclaimed in the acetoacetic ester extraction, and more purified, getting white crystals is resveratrol 1.80g.
Embodiment 3
Get bright giant knotweed II, to contain resveratrol (not comprising glycoside) be 0.19g to 100g after measured, takes by weighing 500g, Pulverize pulping, 20 ± 2 ℃ of lower placements 96 hours, stirred 10 minutes in per 3 hours in the put procedure, use Ethyl acetate extraction reclaims solvent, and more purified, getting white crystals is resveratrol 2.02g. If will This giant knotweed slurry is directly used ethyl acetate extraction, by the same procedure purifying, gets resveratrol 0.65g.
Embodiment 4
Get bright giant knotweed II, to contain resveratrol (not comprising glycoside) be 0.19g to 100g after measured, takes by weighing 500g, Pulverize pulping, 20 ± 2 ℃ of lower placements 96 hours, stirred 10 minutes in per 3 hours in the put procedure, use Ethyl acetate extraction reclaims solvent, and more purified, getting white crystals is resveratrol 2.01g.
Embodiment 5
Get bright giant knotweed III, to contain resveratrol (not comprising glycoside) be 0.15g to 100g after measured, takes by weighing 500g, Pulverize pulping, 35 ± 2 ℃ of lower placements 96 hours, stirred 5 minutes in per 2 hours in the put procedure, use second Solvent is reclaimed in the acetoacetic ester extraction, and more purified, getting white crystals is resveratrol 1.75g. If should The giant knotweed slurry is directly used ethyl acetate extraction, by the same procedure purifying, gets resveratrol 0.55g.
Embodiment 6
Get bright giant knotweed III, to contain resveratrol (not comprising glycoside) be 0.15g to 100g after measured, takes by weighing 500g, Pulverize pulping, 25 ± 2 ℃ of lower placements 120 hours, stirred 5 minutes in per 2 hours in the put procedure, use Ethyl acetate extraction reclaims solvent, and more purified, getting white crystals is resveratrol 1.81g.
Embodiment 7
Get bright giant knotweed IV, to contain resveratrol (not comprising glycoside) be 0.12g to 100g after measured, takes by weighing 500g, Pulverize pulping, 35 ± 2 ℃ of lower placements 96 hours, stirred 5 minutes in per 2 hours in the put procedure, use second Solvent is reclaimed in the acetoacetic ester extraction, and more purified, getting white crystals is resveratrol 1.40g. If should The giant knotweed slurry is directly used ethyl acetate extraction, by the same procedure purifying, gets resveratrol 0.45g.
Embodiment 8
Get bright giant knotweed IV, to contain resveratrol (not comprising glycoside) be 0.12g to 100g after measured, takes by weighing 500g, Pulverize pulping, 25 ± 2 ℃ of lower placements 120 hours, stirred 5 minutes in per 2 hours in the put procedure, use Ethyl acetate extraction reclaims solvent, and more purified, getting white crystals is resveratrol 1.45g.
Claims (1)
1. method that from giant knotweed, prepares trans-resveratrol, it is characterized in that, comprise following processing step, at first, place container fresh giant knotweed making beating, placed 24~120 hours down at 10~50 ℃, and stir, use ethyl acetate extraction then, reclaim ethyl acetate, through being further purified processing, make trans-resveratrol again.
Priority Applications (1)
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CN 01126769 CN1341587A (en) | 2001-09-17 | 2001-09-17 | Method for preparing resveratrol from giant knotweed rhizome |
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CN 01126769 CN1341587A (en) | 2001-09-17 | 2001-09-17 | Method for preparing resveratrol from giant knotweed rhizome |
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CN1341587A true CN1341587A (en) | 2002-03-27 |
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CN 01126769 Pending CN1341587A (en) | 2001-09-17 | 2001-09-17 | Method for preparing resveratrol from giant knotweed rhizome |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100340536C (en) * | 2002-12-31 | 2007-10-03 | 曹庸 | Technology of super critical caron dioxide extraction of resveratrol from polygonum cuspidatum |
CN100436392C (en) * | 2005-12-31 | 2008-11-26 | 曹庸 | Method of culturing and extracting veralkaol using polygonum cuspidatum produced verakaol endogenic bacteria |
CN1896256B (en) * | 2006-06-29 | 2010-07-28 | 大连理工大学 | Method for converting resveratrolside into resveratrol by microbion |
CN101805755A (en) * | 2010-04-14 | 2010-08-18 | 卢昶年 | Process for extracting and purifying resveratrol from giant knot weed |
CN102250969A (en) * | 2011-05-16 | 2011-11-23 | 张家界湘汇生物有限责任公司 | Process for preparing high-purity resveratrol from giant knotweed |
CN101348805B (en) * | 2007-07-20 | 2011-11-30 | 上海诺德生物实业有限公司 | Process for extracting resveratrol from plant |
-
2001
- 2001-09-17 CN CN 01126769 patent/CN1341587A/en active Pending
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100340536C (en) * | 2002-12-31 | 2007-10-03 | 曹庸 | Technology of super critical caron dioxide extraction of resveratrol from polygonum cuspidatum |
CN100436392C (en) * | 2005-12-31 | 2008-11-26 | 曹庸 | Method of culturing and extracting veralkaol using polygonum cuspidatum produced verakaol endogenic bacteria |
CN1896256B (en) * | 2006-06-29 | 2010-07-28 | 大连理工大学 | Method for converting resveratrolside into resveratrol by microbion |
CN101348805B (en) * | 2007-07-20 | 2011-11-30 | 上海诺德生物实业有限公司 | Process for extracting resveratrol from plant |
CN101805755A (en) * | 2010-04-14 | 2010-08-18 | 卢昶年 | Process for extracting and purifying resveratrol from giant knot weed |
CN101805755B (en) * | 2010-04-14 | 2012-01-25 | 卢昶年 | Process for extracting and purifying resveratrol from giant knot weed |
CN102250969A (en) * | 2011-05-16 | 2011-11-23 | 张家界湘汇生物有限责任公司 | Process for preparing high-purity resveratrol from giant knotweed |
CN102250969B (en) * | 2011-05-16 | 2013-01-16 | 张家界湘汇生物有限责任公司 | Process for preparing high-purity resveratrol from giant knotweed |
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