CN1341587A - Method for preparing resveratrol from giant knotweed rhizome - Google Patents

Method for preparing resveratrol from giant knotweed rhizome Download PDF

Info

Publication number
CN1341587A
CN1341587A CN 01126769 CN01126769A CN1341587A CN 1341587 A CN1341587 A CN 1341587A CN 01126769 CN01126769 CN 01126769 CN 01126769 A CN01126769 A CN 01126769A CN 1341587 A CN1341587 A CN 1341587A
Authority
CN
China
Prior art keywords
resveratrol
giant knotweed
ethyl acetate
hours
trans
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN 01126769
Other languages
Chinese (zh)
Inventor
侯团章
曾建国
梁桦
曾洪斌
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SHANGHAI HUIGU BIOLOGICAL TECHNOLOGY Co Ltd
Original Assignee
SHANGHAI HUIGU BIOLOGICAL TECHNOLOGY Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SHANGHAI HUIGU BIOLOGICAL TECHNOLOGY Co Ltd filed Critical SHANGHAI HUIGU BIOLOGICAL TECHNOLOGY Co Ltd
Priority to CN 01126769 priority Critical patent/CN1341587A/en
Publication of CN1341587A publication Critical patent/CN1341587A/en
Pending legal-status Critical Current

Links

Images

Landscapes

  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

The method for preparing resveratrol by using bushy knotweed root includes the following steps: using fresh bushy knotweed root and pulping, placing the obtained pulp material in a container, storing for 24-120 hr. at 10-50 deg.C, and stirring, then using ethyl acetate to make extraction, recovering ethyl acetate, further making purification treatment so as to obtain resveratrol. Because the resveratrol glycoside being in the bushy knotweed root can be converted into resveratrol under the condition without foreign enzyme added. so it has the advantages of simple operation, high yield and low cost.

Description

A kind of method that from giant knotweed, prepares from veratryl alcohol
Technical field
The present invention relates to a kind of from giant knotweed the method for extraction separation trans-resveratrol.
Background technology
Contain trans-resveratrol (resveratrol) and glycoside thereof in the giant knotweed, trans-resveratrol has reducing blood-fat, anti-oxidant, microcirculation improvement, hypotensive and suppress effects such as staphylococcus, suis.
The method of extracting trans-resveratrol mainly contains two classes, and a class at first adopts the interpolation prozyme that the glycoside of trans-resveratrol is carried out enzymolysis, and with solvent extraction, separation and purifying, preparation cost is higher again.Another kind ofly directly extract with solvent, with component separating such as trans-resveratrol glycoside, product yield is low.
Summary of the invention
Purpose of the present invention is exactly to provide the method for preparing trans-resveratrol from giant knotweed that a kind of technology is simple, cost is low, recovery rate is high for the defective that overcomes above-mentioned prior art existence.
Purpose of the present invention can be achieved through the following technical solutions: a kind of method for preparing trans-resveratrol from giant knotweed, it is characterized in that, comprise following processing step, at first, place container fresh giant knotweed making beating, placed 24~120 hours down at 10~50 ℃, and stir, use ethyl acetate extraction then, reclaim ethyl acetate, through being further purified processing, make trans-resveratrol again.
Adopt identical giant knotweed to compare test with the present invention and existing direct extraction method respectively, test-results shows that the recovery rate of the trans-resveratrol that the present invention makes can improve more than 2 times than direct extraction method.The present invention since giant knotweed in the trans-resveratrol glycoside under the situation that need not add exogenous enzyme, be converted into trans-resveratrol, thereby have simple to operate, recovery rate is high, low cost and other advantages.
Description of drawings
Fig. 1 is a process flow sheet of the present invention.
The specific embodiment
Tie below specific embodiment and accompanying drawing, the invention will be further described.
Embodiment 1
Get bright giant knotweed I, to contain resveratrol (not comprising glycoside) be 0.16g to 100g after measured, takes by weighing 500g, Pulverize pulping, 30 ± 2 ℃ of lower placements 96 hours, per hour stirred in the put procedure 5 minutes, use acetic acid Solvent is reclaimed in the ethyl ester extraction, and more purified, getting white crystals is resveratrol 1.89g. If should tiger The cane slurry is directly used ethyl acetate extraction, by the same procedure purifying, gets resveratrol 0.61g.
Embodiment 2
Get bright giant knotweed I, to contain resveratrol (not comprising glycoside) be 0.16g to 100g after measured, takes by weighing 500g, Pulverize pulping, 40 ± 2 ℃ of lower placements 72 hours, stirred 5 minutes in per 2 hours in the put procedure, use second Solvent is reclaimed in the acetoacetic ester extraction, and more purified, getting white crystals is resveratrol 1.80g.
Embodiment 3
Get bright giant knotweed II, to contain resveratrol (not comprising glycoside) be 0.19g to 100g after measured, takes by weighing 500g, Pulverize pulping, 20 ± 2 ℃ of lower placements 96 hours, stirred 10 minutes in per 3 hours in the put procedure, use Ethyl acetate extraction reclaims solvent, and more purified, getting white crystals is resveratrol 2.02g. If will This giant knotweed slurry is directly used ethyl acetate extraction, by the same procedure purifying, gets resveratrol 0.65g.
Embodiment 4
Get bright giant knotweed II, to contain resveratrol (not comprising glycoside) be 0.19g to 100g after measured, takes by weighing 500g, Pulverize pulping, 20 ± 2 ℃ of lower placements 96 hours, stirred 10 minutes in per 3 hours in the put procedure, use Ethyl acetate extraction reclaims solvent, and more purified, getting white crystals is resveratrol 2.01g.
Embodiment 5
Get bright giant knotweed III, to contain resveratrol (not comprising glycoside) be 0.15g to 100g after measured, takes by weighing 500g, Pulverize pulping, 35 ± 2 ℃ of lower placements 96 hours, stirred 5 minutes in per 2 hours in the put procedure, use second Solvent is reclaimed in the acetoacetic ester extraction, and more purified, getting white crystals is resveratrol 1.75g. If should The giant knotweed slurry is directly used ethyl acetate extraction, by the same procedure purifying, gets resveratrol 0.55g.
Embodiment 6
Get bright giant knotweed III, to contain resveratrol (not comprising glycoside) be 0.15g to 100g after measured, takes by weighing 500g, Pulverize pulping, 25 ± 2 ℃ of lower placements 120 hours, stirred 5 minutes in per 2 hours in the put procedure, use Ethyl acetate extraction reclaims solvent, and more purified, getting white crystals is resveratrol 1.81g.
Embodiment 7
Get bright giant knotweed IV, to contain resveratrol (not comprising glycoside) be 0.12g to 100g after measured, takes by weighing 500g, Pulverize pulping, 35 ± 2 ℃ of lower placements 96 hours, stirred 5 minutes in per 2 hours in the put procedure, use second Solvent is reclaimed in the acetoacetic ester extraction, and more purified, getting white crystals is resveratrol 1.40g. If should The giant knotweed slurry is directly used ethyl acetate extraction, by the same procedure purifying, gets resveratrol 0.45g.
Embodiment 8
Get bright giant knotweed IV, to contain resveratrol (not comprising glycoside) be 0.12g to 100g after measured, takes by weighing 500g, Pulverize pulping, 25 ± 2 ℃ of lower placements 120 hours, stirred 5 minutes in per 2 hours in the put procedure, use Ethyl acetate extraction reclaims solvent, and more purified, getting white crystals is resveratrol 1.45g.

Claims (1)

1. method that from giant knotweed, prepares trans-resveratrol, it is characterized in that, comprise following processing step, at first, place container fresh giant knotweed making beating, placed 24~120 hours down at 10~50 ℃, and stir, use ethyl acetate extraction then, reclaim ethyl acetate, through being further purified processing, make trans-resveratrol again.
CN 01126769 2001-09-17 2001-09-17 Method for preparing resveratrol from giant knotweed rhizome Pending CN1341587A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 01126769 CN1341587A (en) 2001-09-17 2001-09-17 Method for preparing resveratrol from giant knotweed rhizome

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 01126769 CN1341587A (en) 2001-09-17 2001-09-17 Method for preparing resveratrol from giant knotweed rhizome

Publications (1)

Publication Number Publication Date
CN1341587A true CN1341587A (en) 2002-03-27

Family

ID=4666774

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 01126769 Pending CN1341587A (en) 2001-09-17 2001-09-17 Method for preparing resveratrol from giant knotweed rhizome

Country Status (1)

Country Link
CN (1) CN1341587A (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100340536C (en) * 2002-12-31 2007-10-03 曹庸 Technology of super critical caron dioxide extraction of resveratrol from polygonum cuspidatum
CN100436392C (en) * 2005-12-31 2008-11-26 曹庸 Method of culturing and extracting veralkaol using polygonum cuspidatum produced verakaol endogenic bacteria
CN1896256B (en) * 2006-06-29 2010-07-28 大连理工大学 Method for converting resveratrolside into resveratrol by microbion
CN101805755A (en) * 2010-04-14 2010-08-18 卢昶年 Process for extracting and purifying resveratrol from giant knot weed
CN102250969A (en) * 2011-05-16 2011-11-23 张家界湘汇生物有限责任公司 Process for preparing high-purity resveratrol from giant knotweed
CN101348805B (en) * 2007-07-20 2011-11-30 上海诺德生物实业有限公司 Process for extracting resveratrol from plant

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100340536C (en) * 2002-12-31 2007-10-03 曹庸 Technology of super critical caron dioxide extraction of resveratrol from polygonum cuspidatum
CN100436392C (en) * 2005-12-31 2008-11-26 曹庸 Method of culturing and extracting veralkaol using polygonum cuspidatum produced verakaol endogenic bacteria
CN1896256B (en) * 2006-06-29 2010-07-28 大连理工大学 Method for converting resveratrolside into resveratrol by microbion
CN101348805B (en) * 2007-07-20 2011-11-30 上海诺德生物实业有限公司 Process for extracting resveratrol from plant
CN101805755A (en) * 2010-04-14 2010-08-18 卢昶年 Process for extracting and purifying resveratrol from giant knot weed
CN101805755B (en) * 2010-04-14 2012-01-25 卢昶年 Process for extracting and purifying resveratrol from giant knot weed
CN102250969A (en) * 2011-05-16 2011-11-23 张家界湘汇生物有限责任公司 Process for preparing high-purity resveratrol from giant knotweed
CN102250969B (en) * 2011-05-16 2013-01-16 张家界湘汇生物有限责任公司 Process for preparing high-purity resveratrol from giant knotweed

Similar Documents

Publication Publication Date Title
US20110271875A1 (en) Process for Recovery of Values from a Fermentation Mass Obtained in Producing Ethanol and Products Thereof
CN105503580B (en) A kind of extraction separation method of nervonic acid
RU2010141585A (en) METHOD AND DEVICE FOR PRELIMINARY PROCESSING OF LIGNO CELLULOSE WITH APPLICATION OF A SUPERFAST SOLVENT CELLULOSE AND EASY VOLVOUS SOLVENTS
CN110205854B (en) Method for extracting nanocellulose and lignin from wood fiber raw material
CN1341587A (en) Method for preparing resveratrol from giant knotweed rhizome
CN101033243A (en) Method of coproducting phytosterol, biological diesel oil and vitamin E
CN1125724A (en) Preparation method of diversine
CN110592292A (en) Pretreatment method for promoting biomass to efficiently extract xylose by mechanical method
CN112709090B (en) Method for preparing cellulose nanofibrils containing lignin
CN1566349A (en) Method for preparing resveratrol from giant knotweed
CN1331342A (en) Process for extracting beta-carotin
CN1072711C (en) Industrial production of highly unsaturated fatty acid
CN109162137B (en) Microcrystalline cellulose and method for preparing microcrystalline cellulose by using crop straws
NL2029555B1 (en) Method for simultaneously extracting phytosterols and polysaccharides from pitaya stems
CN1253446C (en) Method for preparing high content proanthocyanidin
CN109705184A (en) A kind of method that high-purity cholesterol is extracted in serialization from fish oil raffinate
CN1100563C (en) Extraction of effective component in Northern cordyceps producing matrix
CN101387622B (en) Simple method for separating and identifying hemicellulose in cottonseed hull
CN101434590A (en) Preparation of salvianolic acid B pure product
CN101054357A (en) Method for extracting total carotinoid from turmeric
CN1699402A (en) Process for extracting yam saponin from yellow ginger hydrolysate
CN1235220A (en) Process for pulping herbaceous plants without pollution
CN1432579A (en) Refining process of chenodeoxcholic acid
CN115215827B (en) Method for preparing eriodictyol from eriodictyol in enrichment mode
CN1687050A (en) Separation method for purifying gibberellin GA3

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication