CN1333249C - Counterflow chromatograph analyzing and separating preparation with micro-emulsion as solvent - Google Patents
Counterflow chromatograph analyzing and separating preparation with micro-emulsion as solvent Download PDFInfo
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- CN1333249C CN1333249C CNB2004100966954A CN200410096695A CN1333249C CN 1333249 C CN1333249 C CN 1333249C CN B2004100966954 A CNB2004100966954 A CN B2004100966954A CN 200410096695 A CN200410096695 A CN 200410096695A CN 1333249 C CN1333249 C CN 1333249C
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- 239000004530 micro-emulsion Substances 0.000 title claims abstract description 53
- 239000002904 solvent Substances 0.000 title claims abstract description 33
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 239000012071 phase Substances 0.000 claims abstract description 38
- 238000004458 analytical method Methods 0.000 claims abstract description 20
- 239000000463 material Substances 0.000 claims abstract description 19
- 238000000926 separation method Methods 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 12
- 239000007791 liquid phase Substances 0.000 claims abstract description 4
- 230000002411 adverse Effects 0.000 claims description 36
- 239000007788 liquid Substances 0.000 claims description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
- 239000004094 surface-active agent Substances 0.000 claims description 15
- 239000004064 cosurfactant Substances 0.000 claims description 9
- QGBSISYHAICWAH-UHFFFAOYSA-N dicyandiamide Chemical compound NC(N)=NC#N QGBSISYHAICWAH-UHFFFAOYSA-N 0.000 claims description 9
- -1 fatty acid ester Chemical class 0.000 claims description 9
- 238000000746 purification Methods 0.000 claims description 8
- KBPLFHHGFOOTCA-UHFFFAOYSA-N 1-Octanol Chemical compound CCCCCCCCO KBPLFHHGFOOTCA-UHFFFAOYSA-N 0.000 claims description 6
- BBMCTIGTTCKYKF-UHFFFAOYSA-N 1-heptanol Chemical compound CCCCCCCO BBMCTIGTTCKYKF-UHFFFAOYSA-N 0.000 claims description 6
- 230000005526 G1 to G0 transition Effects 0.000 claims description 6
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 6
- AMQJEAYHLZJPGS-UHFFFAOYSA-N N-Pentanol Chemical compound CCCCCO AMQJEAYHLZJPGS-UHFFFAOYSA-N 0.000 claims description 6
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 6
- ZSIAUFGUXNUGDI-UHFFFAOYSA-N hexan-1-ol Chemical compound CCCCCCO ZSIAUFGUXNUGDI-UHFFFAOYSA-N 0.000 claims description 6
- IOLCXVTUBQKXJR-UHFFFAOYSA-M potassium bromide Chemical compound [K+].[Br-] IOLCXVTUBQKXJR-UHFFFAOYSA-M 0.000 claims description 6
- JHJLBTNAGRQEKS-UHFFFAOYSA-M sodium bromide Chemical compound [Na+].[Br-] JHJLBTNAGRQEKS-UHFFFAOYSA-M 0.000 claims description 6
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical group [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 claims description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M sodium chloride Inorganic materials [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 5
- 239000003093 cationic surfactant Substances 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- JKXYOQDLERSFPT-UHFFFAOYSA-N 2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-(2-octadecoxyethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethanol Chemical compound CCCCCCCCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO JKXYOQDLERSFPT-UHFFFAOYSA-N 0.000 claims description 3
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 3
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 3
- 229920001214 Polysorbate 60 Polymers 0.000 claims description 3
- 239000013504 Triton X-100 Substances 0.000 claims description 3
- 229920004890 Triton X-100 Polymers 0.000 claims description 3
- 125000000129 anionic group Chemical group 0.000 claims description 3
- 238000004587 chromatography analysis Methods 0.000 claims description 3
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 3
- SFNALCNOMXIBKG-UHFFFAOYSA-N ethylene glycol monododecyl ether Chemical compound CCCCCCCCCCCCOCCO SFNALCNOMXIBKG-UHFFFAOYSA-N 0.000 claims description 3
- 239000000194 fatty acid Substances 0.000 claims description 3
- 229930195729 fatty acid Natural products 0.000 claims description 3
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 3
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 3
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 3
- 229920000053 polysorbate 80 Polymers 0.000 claims description 3
- 239000001103 potassium chloride Substances 0.000 claims description 3
- 235000011164 potassium chloride Nutrition 0.000 claims description 3
- 239000000600 sorbitol Substances 0.000 claims description 3
- RCEAADKTGXTDOA-UHFFFAOYSA-N OS(O)(=O)=O.CCCCCCCCCCCC[Na] Chemical compound OS(O)(=O)=O.CCCCCCCCCCCC[Na] RCEAADKTGXTDOA-UHFFFAOYSA-N 0.000 claims description 2
- 239000003945 anionic surfactant Substances 0.000 claims 1
- 239000002736 nonionic surfactant Substances 0.000 claims 1
- WQQPDTLGLVLNOH-UHFFFAOYSA-M sodium;4-hydroxy-4-oxo-3-sulfobutanoate Chemical class [Na+].OC(=O)CC(C([O-])=O)S(O)(=O)=O WQQPDTLGLVLNOH-UHFFFAOYSA-M 0.000 claims 1
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 229910021645 metal ion Inorganic materials 0.000 abstract 1
- 239000005445 natural material Substances 0.000 abstract 1
- 239000005416 organic matter Substances 0.000 abstract 1
- QIGBRXMKCJKVMJ-UHFFFAOYSA-N Hydroquinone Chemical compound OC1=CC=C(O)C=C1 QIGBRXMKCJKVMJ-UHFFFAOYSA-N 0.000 description 12
- YCIMNLLNPGFGHC-UHFFFAOYSA-N catechol Chemical compound OC1=CC=CC=C1O YCIMNLLNPGFGHC-UHFFFAOYSA-N 0.000 description 12
- 239000012074 organic phase Substances 0.000 description 8
- 238000004185 countercurrent chromatography Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 150000001455 metallic ions Chemical class 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 3
- NHTMVDHEPJAVLT-UHFFFAOYSA-N Isooctane Chemical compound CC(C)CC(C)(C)C NHTMVDHEPJAVLT-UHFFFAOYSA-N 0.000 description 2
- 150000001335 aliphatic alkanes Chemical class 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- JVSWJIKNEAIKJW-UHFFFAOYSA-N dimethyl-hexane Natural products CCCCCC(C)C JVSWJIKNEAIKJW-UHFFFAOYSA-N 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 229920000136 polysorbate Polymers 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- HFQQZARZPUDIFP-UHFFFAOYSA-M sodium;2-dodecylbenzenesulfonate Chemical compound [Na+].CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O HFQQZARZPUDIFP-UHFFFAOYSA-M 0.000 description 2
- OSOSMTZOKLTVFS-UHFFFAOYSA-N S(=O)(=O)(O)C(C(=O)O)CC(=O)O.C(C)C(C[Na])CCCC Chemical compound S(=O)(=O)(O)C(C(=O)O)CC(=O)O.C(C)C(C[Na])CCCC OSOSMTZOKLTVFS-UHFFFAOYSA-N 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 238000013375 chromatographic separation Methods 0.000 description 1
- 239000011365 complex material Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- APSBXTVYXVQYAB-UHFFFAOYSA-M sodium docusate Chemical compound [Na+].CCCCC(CC)COC(=O)CC(S([O-])(=O)=O)C(=O)OCC(CC)CCCC APSBXTVYXVQYAB-UHFFFAOYSA-M 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000002211 ultraviolet spectrum Methods 0.000 description 1
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Abstract
The present invention discloses a counterflow chromatograph analyzing and separating preparation method using micro-emulsion as a solvent, which belongs to a technical range of material analysis and separation preparation. The present invention comprises the implementation steps that a corresponding liquor-liquor two-phase solvent containing micro-emulsion is selected in view of a sample to be separated, and the two-phase solvent is prepared in proportion; one of the liquor-liquor two-phase solvent containing micro-emulsion is used as a fixed phase in a counterflow chromatogram, and the other liquid phase is used as a mobile phase; a material to be separated is analyzed, separated, prepared or purified in the counterflow chromatogram. The present invention has the advantages that the counterflow chromatogram and the micro-emulsion are organically combined, the beneficial conditions of a micro-emulsion system and counterflow chromatogram technique are combined, the separation efficiency is greatly enhanced, and the use range of the counterflow chromatogram is effectively enlarged. The separation preparation process can be continuously carried out, operation is simple and convenient, and thus, the present invention can be widely used for the fields of the analysis, the separation preparation, etc., of metal ions, organic matter, natural substances and biomolecules.
Description
Technical field
The invention belongs to material analysis and technology of preparing scope, particularly a kind of with adverse current chromatogram analysis and the method for separating and preparing of microemulsion as solvent.
Background technology
Countercurrent chromatography is that a kind of liquid liquid distributes isolation technics, is that separating medium, spiral pipe are the diphasic flow space with liquid liquid two-phase system, is used for analysis, separation, preparation or the purification of material.Adverse current chromatogram is divided into high speed adverse current chromatogram, low speed adverse current chromatogram, quadrature adverse current chromatogram, adverse current distribution chromatography etc.It is that with the fundamental difference of other various chromatographic separation technologies it does not adopt any solid-state absorption supporter, thus got rid of fully supporter to the irreversible adsorption of sample, be infected with, the influence of sex change, inactivation etc.It can realize high-purity, high-recovery, the load separation and purification of big preparation amount greatly, is widely used in analysis, separation, preparation and the purification of complex materials such as metallic ion, organic substance, natural materials, biomolecule.
It is the key factor that countercurrent chromatography uses that dicyandiamide solution is selected.Dicyandiamide solution should have the dissolubility preferably to separated material, and making again has certain separation coefficient between the separated material.Dicyandiamide solution is made up of organic phase and water usually, and separated material all will have certain solubleness in two-phase.And some material only is dissolved in organic phase or only is soluble in the aqueous phase, and just being difficult in organic phase and water is to separate, prepare and purify in the adverse current chromatogram of dicyandiamide solution.This does not have solubleness in organic phase as metallic ion, is difficult to separate with the countercurrent chromatography of traditional system.Biological substance such as protein for another example, inapplicable owing to possibility inactivation in general organic phase, though it is and some aqueous solutions of polymers aqueous two-phases can be used as the dicyandiamide solution of biological substance such as protein,, also difficult because viscosity is big and factor such as aftertreatment difficulty.Some organism are arranged again, as organosilane ester and alcohols etc., only be dissolved in organic phase, and be insoluble in water, just be difficult to separate and purify at the countercurrent chromatography of conventional solvent system.Be mainly seen in the application that separates of relevant metallic ion, natural materials and biological substance at present.But the liquid liquid system of microemulsion is applied in the countercurrent chromatography, more high strength to separate the material of the difficult separation of purifying, has not yet to see report.
Summary of the invention
The purpose of this invention is to provide a kind of with the adverse current chromatogram analysis and the method for separating and preparing of microemulsion as solvent.It is characterized in that: key step of the present invention is: 1) at separated sample, select to contain accordingly the liquid liquid two-phase solvent of microemulsion; 2) select and the corresponding ratio kind of two-phase solvent, dispose the selected liquid liquid two-phase solvent that contains microemulsion; 3) will contain one in the liquid liquid two-phase solvent of microemulsion as the stationary phase in the adverse current chromatogram, with the another one liquid phase as moving phase; 4) in adverse current chromatogram, carry out analysis, separation, preparation or the purification of separated material.
More than said microemulsion solvent comprise water-in-oil type and two kinds of forms of oil-in-water type, the heterogeneous dispersed system that microemulsion is made up of immiscible liquid.By in immiscible two-phase, adding surface reactive material and cosurfactant, just can obtain transparent or semitransparent shape microemulsion, this is a thermodynamic stable system.Owing to have water and oil phase simultaneously in the microemulsion, so they have good dissolution characteristics.At a micro emulsion drop that contains an other phase in mutually, its diameter is between 1 to 100 nanometer, and surfactant is arranged around the drop.Difference according to the property quality and quantity of organism, water and surfactant can form two kinds of microemulsion form of water-in-oil type and oil-in-water type respectively.Water in oil microemulsion system and water and water oil-packaging type micro-emulsion liquid system and oil phase further can form the liquid-liquid equilibrium system.
The common organism that is used to form microemulsion is nonpolar as organic alkane; Surfactant comprises anionic surface active agent such as two (2-ethylhexyl) sodium sulfosuccinate AOT, lauryl sodium sulfate SDS and neopelex SDBS etc., cationic surfactant such as hexadecyl trimethyl ammonium bromide CTAB etc., non-ionics is a kind of of AEO type Brij35 and Brij700, or the fatty acid ester of anhydrous sorbitol is tween series Tween-10, Tween-20, Tween-40, Tween-60, a kind of among Tween-80 and the Tween-85, or a kind of among two NPE DNP-8 and the Triton X-100; Cosurfactant is a kind of in normal butyl alcohol, n-amyl alcohol, n-hexyl alcohol, n-heptanol or n-octyl alcohol or inorganic salts sodium chloride, potassium chloride, sodium bromide and the potassium bromide.The concentration range of organism in system is the concentration 0.01%-10wt% of 20-70wt% surfactant and cosurfactant, and in order to realize quick phase-splitting, the concentration range of inorganic salts is 0.1%-10wt%.
More than said adverse current chromatogram comprise high speed adverse current chromatogram, low speed adverse current chromatogram, X-axis CPC, adverse current distribution chromatography etc.The operating conditions of adverse current chromatogram comprises: the range of speeds 10-1000 of chromatographic column rev/min, and temperature range 0-100 ℃.
The invention has the beneficial effects as follows the characteristics of the high-resolution Separation ﹠ Purification of adverse current chromatogram and the characteristics of dissolubility and high percentage extraction etc. widely of microemulsion solvent are organically combined, enlarged the usable range of adverse current chromatogram effectively, improved separation efficiency.The more foregoing approach that separates between the material of water or organic phase that is insoluble to also is provided.This separation preparation process can be carried out continuously, easy and simple to handle, the advantage of microemulsion system and countercurrent chromatography is combined, has improved separation efficiency widely, and the analysis that can be widely used in metallic ion, organic substance, natural materials and biomolecule with separate fields such as preparation.
Description of drawings
Fig. 1 is the adverse current chromatogram figure (mobile phase flow rate is 0.4 ml/min) of catechol and p-dihydroxy-benzene.
Fig. 2 is the adverse current chromatogram figure (mobile phase flow rate is 0.5 ml/min) of catechol and p-dihydroxy-benzene.
Embodiment
The invention provides a kind of with the adverse current chromatogram analysis and the method for separating and preparing of microemulsion as solvent.Implementation step is: 1) at separated sample, select to contain accordingly the liquid liquid two-phase solvent of microemulsion; 2) select and the corresponding ratio kind of two-phase solvent, dispose the selected liquid liquid two-phase solvent that contains microemulsion; 3) will contain one in the liquid liquid two-phase solvent of microemulsion as the stationary phase in the adverse current chromatogram, with the another one liquid phase as moving phase; 4) in adverse current chromatogram, carry out analysis, separation, preparation or the purification of separated material.
More than said microemulsion solvent comprise water-in-oil type and two kinds of forms of oil-in-water type, the heterogeneous dispersed system that microemulsion is made up of immiscible liquid.By in immiscible two-phase, adding surface reactive material and cosurfactant, just can obtain transparent or semitransparent shape microemulsion, this is a thermodynamic stable system.Owing to have water and oil phase simultaneously in the microemulsion, so they have good dissolution characteristics.At a micro emulsion drop that contains an other phase in mutually, its diameter is between 1 to 100 nanometer, and surfactant is arranged around the drop.Difference according to the property quality and quantity of organism, water and surfactant can form two kinds of microemulsion form of water-in-oil type and oil-in-water type respectively.Water in oil microemulsion system and water and water oil-packaging type micro-emulsion liquid system and oil phase further can form the liquid-liquid equilibrium system.
The common organism that is used to form microemulsion is nonpolar as organic alkane; Surfactant comprises anionic surface active agent such as AOT, SDS and SDBS, cationic surfactant such as hexadecyl trimethyl ammonium bromide CTAB etc., non-ionics is that the fatty acid ester of a kind of or anhydrous sorbitol of AEO type Brij35 and Brij700 is tween series Tween-10, Tween-20, Tween-40, Tween-60, a kind of among a kind of or two NPE DNP-8 among Tween-80 and the Tween-85 and the Triton X-100.Cosurfactant is a kind of in normal butyl alcohol, n-amyl alcohol, n-hexyl alcohol, n-heptanol or n-octyl alcohol or inorganic salts sodium chloride, potassium chloride, sodium bromide and the potassium bromide.The concentration range of organism in system is 20-70wt% (mass percent), the concentration 0.01-10wt% of surfactant and cosurfactant, and in order to realize quick phase-splitting, the concentration range of inorganic salts is 0.1-10wt%.
Only make the embodiment sample separation below, specify embodiments of the present invention with catechol and these two kinds of isomerss of p-dihydroxy-benzene.
Need to prove that the microemulsion type among the embodiment is the water in oil microemulsion of AOT as surfactant, the microemulsion ratio range is: the mass ratio of AOT/ isooctane/water is 1: 16: 16～1: 33: 33, and makes microemulsion system be more suitable for separating with adverse current chromatogram by the method with salt.The range of speeds is 400～500 rev/mins, and flow rates is 0.4～0.5 ml/min.
Adopt AOT: isooctane: water: sodium chloride=1: 16: 16: the solvent system of 0.7 (weight ratio), stirring fully and mix, after the static phase-splitting, is stationary phase with the organic phase, water is mobile, and the potpourri of water-soluble catechol and p-dihydroxy-benzene is carried out separating of adverse current chromatogram.Adopt the GS10A2 type high-speed counter-current chromatograph of Beijing New Technology Institute, wherein separating column is that internal diameter is 1.6 millimeters a polyfluortetraethylene pipe, and 220 milliliters of total measurement (volume)s are equipped with NS-1007 type single cylinder plunger pump and 8823A type UV-detector.Make earlier in the counter-current chromatograph pillar to be full of stationary phase, injection rate IR is 220 milliliters, and the main frame of adverse current chromatogram is rotated, and rotating speed is 400 rev/mins, moving phase is continuously pumped in the post flow 0.4 ml/min again.After treating baseline stability, recording the stationary phase retention volume is 110 milliliters.The aqueous solution that is dissolved with catechol and p-dihydroxy-benzene is injected injection port, and sample size is 5 milliliters (containing each 0.025 gram of catechol and p-dihydroxy-benzene).Ultraviolet spectrum (wavelength 280nm) online record spectrogram is seen Fig. 1.Sample introduction was shut down after 7 hours.The control indoor temperature is 25 ℃.
With embodiment 1, changing mobile phase flow rate is 0.5 ml/min, and the record spectrogram is seen Fig. 2.
The foregoing description shows that the dicyandiamide solution that microemulsion can successfully be used as adverse current chromatogram carries out analysis, separation, preparation and the purification of material.
Claims (6)
1. one kind with adverse current chromatogram analysis and the method for separating and preparing of microemulsion as solvent, and the liquid liquid two-phase solvent of described microemulsion is meant that at the organism sample that separates be the liquid liquid two-phase solvent that contains microemulsion of the ratio preparation of water by the organism that accounts for dicyandiamide solution 20-70wt%, the surfactant that accounts for dicyandiamide solution 0.01-10wt%, the cosurfactant that accounts for dicyandiamide solution 0.01-10wt% and surplus; It is characterized in that: 1) select and the corresponding ratio kind of two-phase solvent, dispose the selected liquid liquid two-phase solvent that contains microemulsion; 2) will contain one in the liquid liquid two-phase solvent of microemulsion as the stationary phase in the adverse current chromatogram, with the another one liquid phase as moving phase, the arbitrary micro emulsion drop that contains an other phase mutually in the two-phase; And surfactant and cosurfactant are arranged around the drop; 3) in adverse current chromatogram, carry out analysis, separation, preparation or the purification of separated material; The range of speeds of the chromatography column that 4) separates in adverse current chromatogram is 400-500 rev/min, and temperature is 25 ℃.
2. described with adverse current chromatogram analysis and the method for separating and preparing of microemulsion as solvent according to claim 1, it is characterized in that: described surfactant is cationic surfactant, anionic surfactant or non-ionic surfactant.
3. described with adverse current chromatogram analysis and the method for separating and preparing of microemulsion as solvent according to claim 2, it is characterized in that: described anionic surface active agent is for one of in two sodium sulfosuccinates, lauryl sodium sulfate and the neopelex.
4. described with adverse current chromatogram analysis and the method for separating and preparing of microemulsion as solvent according to claim 2, it is characterized in that: described cationic surfactant is a hexadecyl trimethyl ammonium bromide.
5. described with the adverse current chromatogram analysis and the method for separating and preparing of microemulsion as solvent according to claim 2, it is characterized in that: non-ionics is the Tween-10 of fatty acid ester of a kind of or anhydrous sorbitol of AEO type Brij35 and Brij700, Tween-20, Tween-40, Tween-60, a kind of among a kind of or two NPE DNP-8 among Tween-80 and the Tween-85 and the Triton X-100.
6. described with adverse current chromatogram analysis and the method for separating and preparing of microemulsion as solvent according to claim 1, it is characterized in that: described cosurfactant is a kind of in normal butyl alcohol, n-amyl alcohol, n-hexyl alcohol, n-heptanol or n-octyl alcohol or inorganic salts sodium chloride, potassium chloride, sodium bromide and the potassium bromide.
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| CN102430265B (en) * | 2011-09-26 | 2014-06-04 | 武汉大学 | Aqueous two-phase extraction system of mixed surfactant |
| CN103115971B (en) * | 2012-11-27 | 2014-08-20 | 广西中烟工业有限责任公司 | Detection method of synthetic phenol antioxidants in flavors and fragrances for cigarettes |
| CN104237416B (en) * | 2014-09-27 | 2015-08-26 | 中国人民解放军兰州军区乌鲁木齐总医院 | The HPLC method of 6 kinds of flavones ingredient content in Simultaneously test poacynum hendersonii woodson leaf |
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| JPH08134069A (en) * | 1994-11-07 | 1996-05-28 | Teijin Ltd | Purification of buprenorphine hydrochloride |
| JP3270725B2 (en) * | 1997-09-30 | 2002-04-02 | 住友重機械工業株式会社 | Roll alignment adjustment device for rolling mill |
| CN1277068A (en) * | 1999-06-14 | 2000-12-20 | 北京市新技术应用研究所 | Highspeed counter-current chromatographic separation process for preparing high purity catechin |
| CN1153603C (en) * | 2000-11-13 | 2004-06-16 | 中国科学院化工冶金研究所 | Process for increasing extraction rate and back-extraction rate of anti-coagulation relative biologic substance by adding aldone ester |
| CN1490319A (en) * | 2003-07-28 | 2004-04-21 | 浙江一新制药股份有限公司 | Method for separating high-purity galanthamine from short-tube lycoris crude extract |
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