CN1331745A - Gene and proteins, and their use - Google Patents

Abstract

According to the present invention, a series of genes are identified in group B streptococcus, the products of which may be associated with the outer surface of the organism. The genes, or functional fragments thereof, may be useful in the preparation of therapeutics, e.g. vaccines to immunise a patient against microbial infection.

Description

Gene and protein, and their purposes

Invention field

The present invention relates to bacterial gene and proteinic evaluation and their purposes.Particularly, relate to its purposes in immunotherapy and drug screening

Background of invention

B group streptococcus (Streptococcus) (GBS) is also referred to as streptococcus agalactiae (Streptococcusagalactiae), is the pathogenic agent of various disease conditions.Particularly, GBS causes;

The early onset infection of newborn

This infection starts from the uterus usually, and causes serious septicemia and pneumonia in the infant, if treatment then it is fatal, even and would treat that it is also relevant with the mortality ratio of 10-20%.

The infection of newborn of tardy property

This infection occurs in for some time at about 3 monthly ages after birth soon.It causes septicemia, is accompanied with meningitis in 90% case.Other focal infection also takes place, comprise osteomyelitis, septic arthritis, abscess and endophthalmitis.

The adult infects

It seems that these are more widespread, occur in harsh product baby's women continually, the philtrum of older and non-responsiveness.They are characterised in that septicemia and focal infection, comprise osteomyelitis, septic arthritis, abscess and endophthalmitis.

Urinary tract infection

GBS is a reason of urinary tract infection, and accounts for 10% of all infection at gestation time.

The animal doctor infects

GBS causes chronic mastitis in cow.This causes milk crop to reduce again, and has therefore subdued sizable economic worth

Can infect with antibiotic therapy GBS.Yet immunization is preferred.Therefore wish a kind of immunogen that can be used in the effective vaccine of treatment of development.

Summary of the invention

The present invention is based on the evaluation of series of genes among the GBS, and relevant with organism, and its product can be positioned at the outside surface of organism and therefore can be used as the target use of immunity-treatment.

According to an aspect of the present invention, peptide be by can from B group connect obtain the coccus comprise arbitrary pho1-13 of being accredited as, pho3-21, pho2-15, pho3-18, pho3-22, pho3-3, pho3-17, pho2-2, pho1-5, pho3-1, pho3-23, pho3-50, pho1-14, pho2-10, pho3-14, the operon of the gene of pho3-24 and pho3-29 or its homologue or its functional fragment coding.This peptide is suitable for therepic use, for example, and when separating.

Use term " functional fragment " to keep the part of complete genome or active gene of peptide or peptide with definition herein.For example, the functional fragment of peptide can be used as the antigen determinative and uses, and it is useful in the production of vaccine or antibody.

Gene fragment can be used to the bioactive peptide of encoding.Perhaps, gene fragment may be useful in gene therapy, and target is decided wild type gene with the performance therapeutic action in vivo.

Peptide of the present invention can comprise and is accredited as SEQ ID NOS herein.2,4,6,8,10,11,13,15,17,19,21,23,25,27,29,31, arbitrary aminoacid sequence of 33 and 35, or its functional fragment.

Because extracellular or cell surface location, for the production of the effective vaccine of treatment of anti-GBS, peptide of the present invention may be the material standed for that suits.The meaning of term " treatment effectively " is the prophylactic effect that comprises vaccine.For example, vaccine can comprise the peptide of the present invention that is used for treatment of infection, or is used for the instrument of its expression.Can give the women with vaccine before pregnancy or in the conceived process and avoid the GBS infection with protection mother and newborn infant.

According to a further aspect in the invention, peptide or gene can be used to screen the potential antimicrobial agents or be used for toxic detecting.

Another aspect of the invention is that arbitrary product of herein identifying is used for and the treatment of B group streptococcus strain infection associated conditions or the purposes of prevention.

Though the purposes of protein in patient treatment described, think product of the present invention veterinary purpose also within the scope of the invention.Particularly, peptide or vaccine can be used for chronic mastitis, particularly the treatment in cow.

Invention is described

The present invention describes according to B group streptococcus bacterial strain M732.Yet all GBS bacterial strains and many other bacterial isolateses may comprise related peptides or the protein that has with M732 peptide homologous aminoacid sequence.The organism that may comprise such peptide comprises, but be not limited to, streptococcus pneumoniae (Streptococcus pneumoniae), streptococcus pyogenes (Streptococcus pyogenes), swine streptococcus (Streptococcussuis), Michaelis suis (Streptococcus milleri), C group and G group streptococcus and faecalis.The vaccine of every kind of these organisms all can be developed according to the same way as of describing GBS.

Preferably, have greater than 40% and the sequence similarity peptide of identifying herein for the useful peptide of production of vaccine.More preferably, peptide has the sequence similarity greater than 60%.Most preferably, peptide has the sequence similarity much larger than 80%, for example, and 95% similarity.

As long as characterized gene of the present invention, it is possible using gene order to establish homologue in other microbe.May determine whether that also other microbe has similar outside surface product.Can for example retrieve among EMBL or the Genbank and set up the sequence homology thing by at existing database.

Can come purifying and separate peptide of the present invention or protein by methods known in the art.Particularly, as long as identified gene order, it will be possible using recombinant technology expressing gene sequence in suitable host.Can identified activity fragment and homologue, and it is useful in treatment.For example, peptide or their active fragments can use as antigenic determinant in vaccine, to cause immunne response.They also can use in the preparation of antibody to be used for passive immunization or diagnostic use, and suitable antibody comprises monoclonal antibody, or its fragment, comprises strand fv fragment.The preparation method of antibody is conspicuous for those skilled in the art.

Preparation based on the vaccine of attenuated microorganisms body is known for those skilled in the art.According to used, needed or desired in the treatment, can use suitable carrier or assistant agent, for example alum is prepared vaccine composition, so that the effective immunization to B group streptococcus or other related microorganism body to be provided.The preparation of vaccine preparation will be conspicuous for the technician.

More generally, and be that those skilled in the art are known,, can select the suitable amount of active ingredient of the present invention, suitable carrier or vehicle, and route of administration for the purposes for the treatment of.Can be according to known standard, as sanatory character/seriousness, patient's type or healthy state etc. are selected or definite these factors.

Following evaluation product of the present invention:

Use plasmid vector pFW-phoA1, pFW-phoA2 and pFW-phoA3 (Podbielski, people such as A, 1996.Gene (Gene)) the portion gene storehouse of preparation GBS (M732 bacterial strain) chromosomal DNA 177:137-147).These plasmids have composition spectinomycin adenylyl transferase antibiotics resistance mark, and it is given high-caliber spectinomycin resistibility and selects therefore and easily.In addition, for alkaline phosphatase, these carriers comprise ((1eaderless) that do not have leading sequence) intestinal bacteria phoA gene of brachymemma.Meet frame BamI restriction enzyme sites with the upstream and compare, these three carriers only just do not have leading sequence phoA gene and whether are present in wherein this respect difference of frame.Because the suitable leader sequence of the intestinal bacteria phoA gene deletion of this brachymemma passes bacterial film to export this enzyme, so when plasmid was bred in intestinal bacteria phoA mutant (for example DH5 α bacterial strain), extracellular alkaline phosphatase activities lacked.Produce the alkaline phosphatase substrate of look, XP (5-bromo-4-chloro-3-indolylphosphate ester) does not enter in the complete bacterial cell, therefore only can detect the alkaline phosphatase activities that is output or be incorporated into the surface.When the alkaline phosphatase activities that is output or is incorporated into the surface exists, produce look XP and be cut with the generation blue pigment, and can be by their blue color identification corresponding bacteria bacterium colony.

With BamHI complete digestion plasmid DNA and use the shrimp alkaline phosphotase dephosphorylation.Partly digest the GBS genomic dna with Sau3AI, on saccharose gradient, carry out fractional separation by size, the fragment of size＜1kb is connected on the prepared pFW-phoA carrier.Coli strain DH5 α is elected to be cloning host, because it lacks functional phoA gene.On the Luria agar that comprises 100 μ g/ml spectinomycins and 40 μ g/ml product look XP substrate, select recombinant plasmid.Comprise plasmid intestinal bacteria transformant that band GBS inserts DNA and be blue color identification by bacterium colony, described insertion DNA complementation do not have an output signal sequence of leading sequence phoA gene.Screened by this way and comprise the about 30000 different recombinant plasmids that GBS inserts DNA, 83 recombinant plasmids of complementary no leading sequence phoA are used for further research.

Select multiple clone from these experiments, every kind all comprises such plasmid, and this plasmid comprises the gene (or its part) of complementary no leading sequence phoA.

In case identified gene in every kind of clone, the so following gene order that obtains complete length is possible.

For the genomic dna order-checking, use the gene fragment design oligonucleotides primer of having identified and having checked order.Design these primers so that from the sequence that obtains, check order with " outside " direction.In case read, check that resulting sequence is to understand 5 ' and the 3 ' end that whether has arrived gene.The existence of these features is by checking the homologue sequence, and for 5 ' end, the existence of formerly AUG initiator codon of Shine-Dalgarno consensus sequence (or the equivalent of generally acknowledging) and for 3 ' end, the existence of translation termination (stopping) codon is identified.

In case the complete length gene identified, for the amplification of the product of complete length and design primer.Employed primer comprises that restriction enzyme recognition site (NcoI is positioned at 5 ' end and Eco0109I is positioned at 3 ' end) is to allow product afterwards be cloned into used galactococcus (Lactococcal) expression system.

Use primer to advance PCR, and product is cloned into pCR2.1 cloning vector (in Vitrogen).After the existence conclusive evidence of cloned sequence, use restriction enzyme NcoI and Eco0109I to cut off DNA.

The carrier that fragment is inserted wherein is pNZ8048 version (Kuipers, people such as 0.P., (1998) biotechnology magazine (J.Biotech)) 64:15-21 who modifies).This carrier has the galactococcus replication orgin, the chlorampenicol resistant mark, derivable nisin promotor and a kind of multiple clone site its by with two 10X His labels to the replacement of multiple clone site and change described label, 5 ' distolateral the wing is the NcoI site, the centre separates (comprising the Eco0109I site) with multiple clone site, and terminator codon is at 3 ' end.

Be inserted with correlation gene, so that 10X His label is positioned at 3 ' with respect to the coding region.Recombinant plasmid transformed is advanced Lactococcus lactis (L.lactis) ((1998) are the same for bacterial strain NZ9000-Kuipers, people such as O.P.) afterwards, prepares the 400ml liquid culture, by nisin being joined in the culture translation of induced protein.After cultivating 2 hours, harvested cell and by bead making beating (bead beating) lysing cell.By the final cell lysate of centrifugal purification, then it is fed metal affinity column (Talon Clonetech).Before with imidazoles elution of bound albumen, the repetitive scrubbing pillar.

In order to identify the recombinant protein fraction that comprises the His label, use SDS-PAGE, the Western engram analysis is also with the aliquots containig of anti-His antibody detection source from each fraction.

Then resulting recombinant protein is used for immune New Zealand white rabbit.Gather in the crops preimmune serum before the immunity.After the booster immunization, put to death white rabbit and collect serum.This serum is used for western blotting, in ELISA and the protection of animal model.

The serum that use obtains from zooscopy carries out the research of immunosorption.

The B group streptococcus was cultivated 8 hours in 20ml Todd Hewitt meat soup (THB), results and resuspension in 5ml PBS.The aliquots containig of 50 μ l is used at 96 orifice plates (Nunc Immuno-Sorb) bag by the hole.Spend the night so that bacterium is adsorbed on the plate at 4 ℃.With PBS wash plate twice, in the time of 37 ℃, in PBS, sealed 1 hour then with 3%BSA.Wash plate again.In PBS, produce the continuous 10 times diluent of serum, and these diluents of 50 μ l are joined in the hole of plate double.Wrapper plate was also cultivated 1 hour in the time of 37 ℃.Wash this plate, the anti-rabbit alkaline phosphatase of 1: 5000 concentration of 50 μ l is puted together secondary antibodies join in each hole.Cultivate after 1 hour, wash this plate again for 37 ℃.50 μ, 1 substrates (PNPP) are joined in each hole, before the absorption value that reads the 405nm place, allow reaction carry out 30 minutes.

The research of also carrying out protection of animal is to test in the protection effect on the rabbit of immunity.

GBS M732 dashes at THB and cultivates up to reaching-about 5 hours stage of mid-log phase.Counting cells in nucleonics, it is 2 * 10 that dilution obtains concentration ⁷The preimmune serum of individual bacterium/ml or test sera.50 μ l are injected in the mouse of 0-1 age in days via the intraperitoneal approach.The survival of observing mouse was above 48 hours.

The following example illustrates the present invention.

Embodiment 1

First clone is included in gene order that is accredited as SEQ ID NO.1 herein and the aminoacid sequence that is accredited as SEQID NO.2, and is classified as pho1-13.

The aminoacid sequence that carries out pho1-13 compares.

Can be streptococcus pyogenes, streptococcus pneumoniae, streptococcus-salivarius (S.salivarius), intestinal bacteria, yersinia entero-colitica (Yersinia enterocolitica), Aquifex aeolicus, the homologue of evaluation GBS pho1-13 gene product in helicobacter pylori (Helicobacter pylori) and the hemophilus influenzae (Haemophilus influenzae).The homologue of streptococcus pyogenes and streptococcus pneumoniae identifies from genomic sequence data, and about the evaluation of gene or gene product, it is available not having note.In other the situation, above-mentioned homologue can be accredited as the Clp protease protein cracking subunit that depends on ATP at all.Exist under the situation at ATP and magnesium, it is little peptide (Giffard, people such as P.M. 1993. general microbiology magazine (J.Gen.Microbiol.) 139:913-920) that the Clp catalytic activity of proteinase causes proteolysis.In addition, the ClpP composition of verified Clp proteolytic enzyme induced for heat shock response a part (Kroh, H.E. and L.D.Simon.1990. bacteriology magazine (J.Bacteriol) .172:6026-6034) and may this subunit or the surface bonding of protein cleavage structural domain and bacterium completely.

According to the above-described immune Research of carrying out, obtain following result.

Treatment does not have animal not have animals survived

Time (hour)

24 48

PBS 10 7 0

Immunity preceding 37 13 0

Through 38 17 9 of immunity

Embodiment 2

Select second and comprise the clone who is called the pho1-14 plasmid.This plasmid comprises the gene (or its part) of complementary no leading sequence phoA.Nucleotide and the aminoacid sequence of inferring are listed as SEQ ID NOS.3 and 4 respectively.

The aminoacid sequence that carries out pho1-14 compares.

Can be streptococcus pyogenes, identify the homologue of GBS pho1-14 gene product in enterococcus faecalis (Enterococcus faecalis) and the streptococcus pneumoniae.These homologues identify from genomic sequence data, and about the evaluation of gene or gene product, it is available not having note.In addition, also can from shigella flexneri (Shigella flexneri) (SpaR) and artificial tuberculosis yersinia genus (Yersinia pseudotuberculosis) identify two kinds of possible homologues (YscT).These back two kinds of homologues are related proteins, think its grappling on the film of bacterium (Bergman, T1994. bacteriology magazine 176:2619-2626).In shigella flexneri, verified spaR gene product is important (Sasakawa, C.et al.1993. bacteriology magazine 175:2334-2346) for epithelial invasion.In addition, the spaR gene product is presented for invading the antigenic surface of plasmid, also needs.In artificial tuberculosis yersinia genus, similarly protein is the integral part of Yop excretory system, and also is important for the toxicity in the organism.

Embodiment 3

Select the 3rd to comprise the clone who is called the pho1-5 plasmid.This plasmid comprises the gene (or its part) of complementary no leading sequence phoA.Nucleotide and the aminoacid sequence of inferring are listed as SEQ ID NOS.5 and 6 respectively.

The aminoacid sequence that carries out pho1-5 compares.

Only can be streptococcus pyogenes, Staphylococcus carnosus (Staphylococcus carnosus) identifies the homologue of GBS pho1-5 gene product in (sceA).The streptococcus pyogenes homologue identifies from genomic sequence data, and about the evaluation of gene or gene product, it is available not having note.In addition, be derived from the sceA gene product of Staphylococcus carnosus, it is utilizable almost not having information.The SceA gene product shows some and the similar sequence of short agrin matter that is derived from Lactobacillus gasseri (Lactobacillus gasseri).Based on the analysis of sceA gene product, this molecule comprise that fully conservative signal sequence and its are secreted obviously or with the surface bonding of bacterial cell.

Embodiment 4

Select another to comprise the clone who is called the pho3-3 plasmid.This plasmid comprises the gene (or its part) that replenishes no leading sequence phoA.Nucleotide and the aminoacid sequence of inferring are listed as SEQ ID NOS.7 and 8 respectively.

The aminoacid sequence that carries out pho3-3 compares.

Can be (rmiC) at Streptococcus mutans (Streptococcus mutans), (cpsM) homologue of evaluation GBS pho3-3 gene product in streptococcus pneumoniae and the streptococcus pyogenes.The streptococcus pyogenes homologue identifies from genomic sequence data, and about the evaluation of gene or gene product, it is available not having note.In streptococcus pneumoniae, homologue can be accredited as dTDP-4-ketone-6-deoxyglucose-3,5-epimerase.Under other two kinds of situations, top homologue can be accredited as dTDP-4-ketone-L-rhamnosyl reductase enzyme (rmlC).In Streptococcus mutans, the rmlC of this enzyme gene of encoding is the part of rml locus.The rml locus comprises three kinds of genes, and it demonstrates and participate in the significant similarity of enzyme (Tsukioka, the people such as Y. in the biosynthesizing of dTDP-rhamnosyl (the direct precursor of rhamnosyl composition in the Streptococcus mutans polyoses capsule).1997. bacteriology magazine .17:73-83).In streptococcus pneumoniae, identify similar locus (people such as coffey, 1998, molecular microbiology, 17:73-83).Nearly all suis characteristic ground in their cell wallss relevant with polysaccharide has rhamnosyl (Schleifer, K.H. with R.Kilper-Balz.1987. system applies microorganism (Syst.Appl.Microbiol) .10: 1-19), and in suis, dTDP-4-ketone-L rhamnosyl reductase enzyme combines with outside surface probably.

Embodiment 5

Select another to comprise the clone who is called the pho2-10 plasmid.This plasmid comprises the gene (or its part) of complementary no leading sequence phoA.

Nucleotide sequence is listed as SEQ ID NO.9 respectively.Thus, identified the upstream and the coding region downstream, the aminoacid sequence of inferring is listed as SEQ ID NOS.10 and 11.

The aminoacid sequence that carries out pho2-10 compares.

Can be streptococcus pyogenes, enterococcus faecalis, the homologue of evaluation GBS pho2-10 gene product in Debaryomyces occidentalis (hatI) and the intestinal bacteria (trkD).Streptococcus pyogenes and intestinal bacteria homologue identify from genomic sequence data, and about the evaluation of gene or gene product, it is available not having note.In yeast D.occidentalis, the hak1 gene is homologue (Banuelos, the people such as M.A. who is derived from colibacillary trkD gene.1995.EMBO?J.14：3012-3027)。Colibacillary trkD gene is the part of kup potassium capturing system.The specific homologue of Jian Dinging is a kup system potassium picked-up albumen herein.In intestinal bacteria, the kup system is a kind of composing type potassium capturing system.Kup system potassium picked-up albumen comprises highly hydrophobic N-end, predicts the leap film at least its 12 times.Kup not with other known membranin sequence homologies.Do not have ATP bonded sign, and prompting comes moving system (Schleyer, M.﹠amp by chemiosmotic gradient; E.P.Bakker, 1993. bacteriology magazine .175:6925-6931).

Embodiment 6

Select another to comprise the clone who is called the pho2-15 plasmid.This plasmid comprises the gene (or its part) of complementary no leading sequence phoA.The Nucleotide of gene and putative amino acid sequence are listed as SEQ ID NOS.12 and 13 respectively.

The aminoacid sequence that carries out pho2-15 compares.

Can be streptococcus pyogenes, identify the homologue of GBS pho2-15 gene product in the streptococcus pneumoniae, enterococcus faecalis and intestinal bacteria (gat C and SgcC).Streptococcus pyogenes, streptococcus pneumoniae and enterococcus faecalis homologue identify from genomic sequence data, and about the evaluation of gene or gene product, it is available not having note.In intestinal bacteria, gatC and sgcC gene product can be accredited as the IIC composition of the sugar phosphotransferase system (PTS) that phosphoenolpyruvic acid relies on (a kind of important carbohydrate activity-transport system).In the PTS system, the IIC composition generally participates in the combination of extracellular carbohydrate, and form a complex body with component film passage (Nobelmann, B. and J.W.Lengeler.1995. biological chemistry and biophysics journal (Biochin.Biophys.Acta) 1262:69-72) with the IID composition.

Embodiment 7

Select another to comprise the clone who is called the pho2-2 plasmid.This plasmid comprises the gene (or its part) of complementary no leading sequence phoA.The Nucleotide of gene and putative amino acid sequence are listed as SEQ ID NOS.14 and 15 respectively.

The aminoacid sequence that carries out pho2-2 compares.

Can be at enterococcus faecalis, intestinal bacteria (malK and afuC), subtilis (glh0), hemophilus influenzae (yebM and potA), streptococcus pyogenes, streptococcus pneumoniae, and Salmonella typhimurium (Salmonella typhimurium) is identified the homologue of GBS pho2-2 gene product in (malK).Enterococcus faecalis, streptococcus pyogenes and streptococcus pneumoniae homologue identify from genomic sequence data, and about the evaluation of gene or gene product, it is available not having note.In other the situation, homologue has been represented ATP-binding transport albumen at all, and it is the part of ABC type translocator.Many compositions of ABC type translocator are to be incorporated into film or cell surface, and these systems participate in the transhipment of macromole from extracellular environment compartment in cell.

Embodiment 8

Select another to comprise that the gram that is called the pho3-14 plasmid falls.This plasmid comprises the gene (or its part) of complementary no leading sequence phoA.The Nucleotide of gene and the aminoacid sequence of inferring are listed as SEQ ID NOS.16 and 17 respectively.

The aminoacid sequence that carries out pho3-14 compares.And there is not homologue in any public data storehouse, to be identified.A homologue of GBSpho3-14 gene product can identify in streptococcus pyogenes, but this homologue identifies that from genomic sequence data and about the evaluation of gene or gene product, it is available not having note.Use this streptococcus pyogenes homologue to retrieve disclosed database, do not obtain more information.Since the complementation of pho3-14 product no leading sequence phoA gene, can infer that this protein (or its part) is positioned at most probable in the extracellular.

Embodiment 9

Select another to comprise the clone who is called the pho3-17 plasmid.This plasmid comprises the gene (or its part) of complementary no leading sequence phoA.The Nucleotide of gene and putative amino acid sequence are listed as SEQ ID NOS.18 and 19 respectively.

The aminoacid sequence that carries out pho3-17 compares.

Can in Streptococcus mutans and Lactococcus lactis, identify the homologue of GBS Pho3-17 gene product, have similarity with N-ethanoyl muramidase.Also notice the similarity that promptly is derived from the yubE of subtilis with unidentified gene.

N-ethanoyl muramidase is a kind of autolysin that participates in the cell fission.Use the fact of this finite information, can infer that the pho3-17 product is positioned at most probable in the extracellular together with the complementary no leading sequence phoA gene of pho3-17.

Embodiment 10

Select another to comprise the clone who is called the pho3-18 plasmid.This plasmid comprises the gene (or its part) of complementary no leading sequence phoA.The Nucleotide of gene and putative amino acid sequence are listed as SEQ ID NOS.20 and 21 respectively.

The aminoacid sequence that carries out pho3-18 compares.

Can in streptococcus pyogenes and streptococcus pneumoniae, identify the homologue of GBS Pho3-18 gene product.These homologues identify from genomic sequence data, and about the evaluation of gene or gene product, it is available not having note.Use these streptococcus pyogeness and streptococcus pneumoniae homologue to retrieve disclosed database, the similarity that it shows some and outside surface and crosses over membranin.Since the complementation of ORF3-18 product no leading sequence phoA gene, can infer that this protein (or its part) is positioned at most probable in the extracellular.

Embodiment 11

Select another to comprise the clone who is called the pho3-1 plasmid.This plasmid comprises the gene (or its part) of complementary no leading sequence phoA.The Nucleotide of gene and putative amino acid sequence are listed as SEQ ID NOS.22 and 23 respectively.

The aminoacid sequence that carries out pho3-1 compares.

Can be streptococcus pyogenes, streptococcus pneumoniae, the homologue of evaluation GBS Pho3-1 gene product in subtilis (yutD) and the enterococcus faecalis.Streptococcus pyogenes, streptococcus pneumoniae and enterococcus faecalis homologue identify from genomic sequence data, and about the evaluation of gene or gene product, it is available not having note.In subtilis, the function of yutD gene product is unknown.Yet can notice that the yutD gene is arranged in subtilis karyomit(e) and contains the zone that participates in cell walls synthetic gene.This dna sequence dna complementation do not have a phoA gene of leading sequence the fact hint that this gene product is positioned at the extracellular.

Embodiment 12

Select another to comprise the clone who is called the pho3-21 plasmid.This plasmid comprises the gene (or its part) of complementary no leading sequence phoA.The Nucleotide of gene and putative amino acid sequence are listed as SEQ ID NOS.24 and 25 respectively.

The aminoacid sequence that carries out pho3-21 compares.

Can be streptococcus pyogenes, streptococcus pneumoniae, lactobacillus fermentum (Lactobacillus fermentum) (bspA) and lactobacillus reuteri (Lactobacillus reuteri) identify the homologue of GBS Pho3-21 gene product in (cnb).Streptococcus pyogenes, streptococcus pneumoniae homologue identify from genomic sequence data, and about the evaluation of gene or gene product, it is available not having note.In lactobacillus fermentum, the bspA gene product has been accredited as (basic) cell surface-localized protein substantially, it has some sequence (Turner, people .1997. bacteriology magazine .179:3310-3316s such as M.S.) similar to bacterium solute-conjugated protein III family.In lactobacillus reuteri, it is conjugated protein that the cnb gene product has been accredited as collagen, and it has some and combines the similar sequence of component (Roos, people 1996.FEMS Microbiol.Lett.144:33-38 such as S.) with bacterium abc transport albumen solute.

Embodiment 13

Select another to comprise the clone who is called the pho3-22 plasmid.This plasmid comprises the gene (or its part) of complementary no leading sequence phoA.The Nucleotide of gene and putative amino acid sequence are listed as SEQ ID NOS.26 and 27 respectively.

The aminoacid sequence that carries out pho3-22 compares.

Can be at enterococcus faecalis, streptococcus equisimilis (Streoticiccys eqyusunukus) (lppC), Pseudomonas fluorescens (Pseudomonas fluorescens) is (oprI) and identify the homologue of GBS Pho3-22 gene product in the thermophilus streptococcus (orfl42).The enterococcus faecalis homologue identifies from genomic sequence data, and about the evaluation of gene or gene product, it is available not having note.In streptococcus equisimilis, the lppC gene product has been accredited as lipoprotein, itself and E (P4) the outer membrane protein homology (Gase, people such as K. 1997 medical microbial immunology (Med.Microbiol.Immunol) .186:63-73) that is derived from hemophilus influenzae.Similarly, important outer membrane lipoprotein (Cornelis, the people such as P. of Pseudomonas fluorescens oprI genes encoding one.1989. molecular microbiology 3:421-428).In thermophilus streptococcus, the orf142 product putatively has been accredited as the lipoprotein that is exposed to cell surface, and it serves as acceptor (Neve, the people such as H. of phage kTP-J34 and Sfi21.1998. virusology (Virology) 241:61-72).The ORF3-22 product has demonstrated and top homologue similarity preferably, particularly holds at N-.This is likely the complementary needed zone of the phoA gene of no leading sequence.And therefore as leader sequence.

Embodiment 14

Select another to comprise the clone who is called the pho3-23 plasmid.This plasmid comprises the gene (or its part) of complementary no leading sequence phoA.The Nucleotide of gene and putative amino acid sequence are listed as SEQ ID NOS.28 and 29 respectively.

Carry out the aminoacid sequence contrast of pho3-23.

Can be streptococcus pyogenes, streptococcus pneumoniae, the homologue of evaluation GBSpho3-23 gene product in enterococcus faecalis and the Streptococcus mutans (perM).Streptococcus pyogenes, streptococcus pneumoniae and enterococcus faecalis homologue identify from genomic sequence data, and about the evaluation of gene or gene product, it is available not having note.In Streptococcus mutans, the perM gene product has been accredited as permease with being assumed to be, but it is available not having out of Memory about this proteinic function.Consider the complementary no leading sequence phoA gene of pho3-23 coding region, can infer that the pho3-17 gene product is positioned at most probable in the extracellular.

Embodiment 15

Select another to comprise the clone who is called the pho3-24 plasmid.This plasmid comprises the gene (or its part) of complementary no leading sequence phoA.The Nucleotide of gene and putative amino acid sequence are listed as SEQ ID NOS.30 and 31 respectively.

The aminoacid sequence that carries out pho3-24 compares.

Can be at Streptococcus mutans (dltB), streptococcus pneumoniae, streptococcus pyogenes, enterococcus faecalis, lactobacterium casei (Lactobaillus casei) are (dltB) and identify the homologue of GBS pho3-24 gene product in the subtilis (dltB).Streptococcus pneumoniae, streptococcus pyogenes and enterococcus faecalis homologue identify from genomic sequence data, and about the evaluation of gene or gene product.It is available not having note.At Streptococcus mutans, in lactobacterium casei and the subtilis, the dltB gene product has been accredited as underlying membrane albumen, and its participation is passed in the transhipment of active D-L-Ala of cytolemma.The dltB gene product participates in the biosynthesizing (Heaton, M.P. and F.C.Neuhaus.1992. bacteriology magazine 174:4707-4717) of D-alanyl-lipoteichoic acid.In lactobacterium casei and subtilis, think that the dltB gene product comprises at least 9 films and crosses over structural domain, show that protein or its part are exposed to the outside of cell.

Embodiment 16

Select another to comprise the clone who is called the pho3-29 plasmid.This plasmid comprises the gene (or its part) of complementary no leading sequence phoA.The Nucleotide of gene and putative amino acid sequence are listed as SEQ ID NOS.32 and 33 respectively.

The aminoacid sequence that carries out pho3-29 compares.

Can B. burgdorferi (Borrelia burgdorferi) (p23 or ospC), bacillus brevis (Bacillus brevis) (owp) and Pseudomonas aeruginosa (Pseudomonas aeruginosa) identify the homologue of GBS pho3-29 gene product in (oprI).Though these homologues are also uncorrelated each other, they all represent important outer surface protein.In B. burgdorferi, the ospC gene product has been accredited as 23-kDa albumen, and it is immunodominant antigen (Padula, people such as S.J. on this bacterium surface.1993 infect and immunity (Infect.Immun) .61:5097-5105).The owp gene product that is derived from bacillus brevis is a kind of (Tsuboi, A.1988. the bacteriology magazine 170:935-945) in two kinds of main cell wall proteins that are included in the upper layer lattice.At last, be derived from the important outer membrane lipoprotein precursor (Saint-Onge, people such as A. 1992 general microbiology magazine (J.Gen.Microbiol) .138:733-741) of oprI genes encoding of Pseudomonas aeruginosa.

Embodiment 17

Select another to comprise the clone who is called the pho3-50 plasmid.This plasmid comprises the gene (or its part) of complementary no leading sequence phoA.The Nucleotide of gene and putative amino acid sequence are listed as SEQ ID NOS.34 and 35 respectively.

The aminoacid sequence that carries out pho3-50 compares.

Can be at suis (penA, pbp2B, pbpB2), B. burgdorferi (pbp2), enterococcus faecalis (pbpC), streptococcus aureus (Stephylococcus aureus) (pbpA), Mycobacterium leprae (Mycobacterium leprae) is (pbpC) and identify the homologue of GBS Pho3-50 gene product in the helicobacter pylori (pbp2).In all situations, top homologue can be accredited as penicillin-binding protein (PBP).The gene of Renicillin binding protein is usually located at cell walls and synthesizes in the relevant cluster gene (Pucci, people .1997. bacteriology magazine 179:5632-5635 such as M.J.).In addition, PBP is in the cell walls of bacterium, and some or all protein is exposed to the general integration of outside bacterium surface.

Sequence table＜110〉Microscience Limited＜120〉gene and protein, and uses thereof＜130〉REP05973WO＜140＜141＜160〉35＜170〉PatentIn Ver.2.1＜210〉1＜211〉587＜212〉DNA＜213〉B group streptococcus＜220〉＜221〉CDS＜222〉(1) .. (582)＜400〉1atg atc cca gta gta atc gaa caa aca agt cgt ggt gaa cgt tct tat 48Met Ile Pro Val Val Ile Glu Gln Thr Ser Arg Gly Glu Arg Ser Tyr 15 10 15gat att tac tca cgt ctt tta aaa gat cgt att att atg ttg aca ggc 96Asp Ile Tyr Ser Arg Leu Leu Lys Asp Arg Ile Ile Met Leu Thr Gly

20 25 30caa?gtt?gag?gat?aat?atg?gcc?aat?agt?atc?att?gca?cag?tta?ttg?ttt 144Gln?Val?Glu?Asp?Asn?Met?Ala?Asn?Ser?Ile?Ile?Ala?Gln?Leu?Leu?Phe

35 40 45ctc?gat?gca?caa?gat?aat?aca?aag?gat?att?tac?ctt?tat?gtc?aat?aca 192Leu?Asp?Ala?Gln?Asp?Asn?Thr?Lys?Asp?Ile?Tyr?Leu?Tyr?Val?Asn?Thr

50 55 60cca?ggt?ggt?tca?gta?tcg?gct?gga?ctt?gct?att?gtg?gac?acc?atg?aac 240Pro?Gly?Gly?Ser?Val?Ser?Ala?Gly?Leu?Ala?Ile?Val?Asp?Thr?Met?Asn?65 70 75 80ttc?att?aaa?tcg?gac?gta?cag?acg?att?gtt?atg?ggg?atg?gct?gct?tcg 288Phe?Ile?Lys?Ser?Asp?Val?Gln?Thr?Ile?Val?Met?Gly?Met?Ala?Ala?Ser

85 90 95atg?gga?acc?att?att?gct?tca?agt?ggt?gct?aaa?gga?aaa?cgt?ttt?atg 336Met?Gly?Thr?Ile?Ile?Ala?Ser?Ser?Gly?Ala?Lys?Gly?Lys?Arg?Phe?Met

100 105 110tta?ccg?aat?gca?gaa?tat?atg?atc?cac?caa?cca?atg?ggc?gga?aca?ggc 384Leu?Pro?Asn?Ala?Glu?Tyr?Met?Ile?His?Gln?Pro?Met?Gly?Gly?Thr?Gly

115 120 125gga?ggt?aca?cag?caa?tct?gat?atg?gct?atc?gct?gct?gag?cat?ctt?tta 432Gly?Gly?Thr?Gln?Gln?Ser?Asp?Met?Ala?Ile?Ala?Ala?Glu?His?Leu?Leu

130 135 140aaa?acg?cgt?cat?act?tta?gaa?aaa?atc?tta?gct?gat?aat?tct?ggt?caa 480Lys?Thr?Arg?His?Thr?Leu?Glu?Lys?Ile?Leu?Ala?Asp?Asn?Ser?Gly?Gln145 150 155 160tct?att?gaa?aaa?gtc?cat?gat?gat?gca?gag?cgt?gat?cgt?tgg?atg?agt 528Ser?Ile?Glu?Lys?Val?His?Asp?Asp?Ala?Glu?Arg?Asp?Arg?Trp?Met?Ser

165 170 175gct?caa?gaa?aca?ctt?gat?tat?ggc?ttt?att?gat?gaa?atc?atg?gct?aat 576Ala?Gln?Glu?Thr?Leu?Asp?Tyr?Gly?Phe?Ile?Asp?Glu?Ile?Met?Ala?Asn

180 185 190aat gaa taagg 587Asn Glu＜210〉2＜211〉194＜212〉PRT＜213〉B group streptococcus＜400〉2Met Ile Pro Val Val Ile Glu Gln Thr Ser Arg Gly Glu Arg Ser Tyr 15 10 15Asp Ile Tyr Ser Arg Leu Leu Lys Asp Arg Ile Ile Met Leu Thr Gly

20 25 30Gln?Val?Glu?Asp?Asn?Met?Ala?Asn?Ser?Ile?Ile?Ala?Gln?Leu?Leu?Phe

35 40 45Leu?Asp?Ala?Gln?Asp?Asn?Thr?Lys?Asp?Ile?Tyr?Leu?Tyr?Val?Asn?Thr

50 55 60Pro?Gly?Gly?Ser?Val?Ser?Ala?Gly?Leu?Ala?Ile?Val?Asp?Thr?Met?Asn?65 70 75 80Phe?Ile?Lys?Ser?Asp?Val?Gln?Thr?Ile?Val?Met?Gly?Met?Ala?Ala?Ser

85 90 95Met?Gly?Thr?Ile?Ile?Ala?Ser?Ser?Gly?Ala?Lys?Gly?Lys?Arg?Phe?Met

100 105 110Leu?Pro?Asn?Ala?Glu?Tyr?Met?Ile?His?Gln?Pro?Met?Gly?Gly?Thr?Gly

115 120 125Gly?Gly?Thr?Gln?Gln?Ser?Asp?Met?Ala?Ile?Ala?Ala?Glu?His?Leu?Leu

130 135 140Lys?Thr?Arg?His?Thr?Leu?Glu?Lys?Ile?Leu?Ala?Asp?Asn?Ser?Gly?Gln145 150 155 160Ser?Ile?Glu?Lys?Val?His?Asp?Asp?Ala?Glu?Arg?Asp?Arg?Trp?Met?Ser

165 170 175Ala?Gln?Glu?Thr?Leu?Asp?Tyr?Gly?Phe?Ile?Asp?Glu?Ile?Met?Ala?Asn

180 185 190Asn Glu＜210〉3＜211〉218＜212〉DNA＜213〉B group streptococcus＜220〉＜221〉CDS＜222〉(1) .. (216)＜400〉3atc aga gca tat tct ggt cct ctt tcg gtt ttc ctg cca cgt ttt aaa 48Ile Arg Ala Tyr Ser Gly Pro Leu Ser Val Phe Leu Pro Arg Phe Lys 15 10 15gct tgt gat ara ata gtc aat gtg agg agg act atc atg tta ttt aag 96Ala Cys Asp Ile Ile Val Asn Va1 Arg Arg Thr Ile Met Leu Phe Lys

20 25 30gaa?aaa?att?cct?gga?cta?ata?tta?tgc?ttt?att?att?gct?ata?cca?tct 144Glu?Lys?Ile?Pro?Gly?Leu?Ile?Leu?Cys?Phe?Ile?Ile?Ala?Ile?Pro?Ser

35 40 45tgg?ttg?ctt?ggg?ctt?tat?ctc?cct?tta?ata?gga?gca?cca?gtc?ttt?gct 192Trp?Leu?Leu?Gly?Leu?Tyr?Leu?Pro?Leu?Ile?Gly?Ala?Pro?Val?Phe?Ala

50 55 60atc ttg att gga ata att gtt gga tc 218Ile Leu Ile Gly Ile Ile Val Gly, 65 70＜210〉4＜211〉72＜212〉PRT＜213〉B group streptococcus＜400〉4Ile Arg Ala Tyr Ser Gly Pro Leu Ser Val Phe Leu Pro Arg Phe Lys 15 10 15Ala Cys Asp Ile Ile Val Asn Val Arg Arg Thr Ile Met Leu Phe Lys

20 25 30Glu?Lys?Ile?Pro?Gly?Leu?Ile?Leu?Cys?Phe?Ile?Ile?Ala?Ile?Pro?Set

35 40 45Trp?Leu?Leu?Gly?Leu?Tyr?Leu?Pro?Leu?Ile?Gly?Ala?Pro?Val?Phe?Ala

50 55 60Ile Leu Ile Gly Ile Ile Val Gly, 65 70＜210〉5＜211〉705＜212〉DNA＜213〉B group streptococcus＜220〉＜221〉CDS＜222〉(1) .. (705)＜400〉5atg aat aaa aga aga aaa tta tca aaa ttg aat gta aaa aar caa cat 48Met Asn Lys Arg Arg Lys Leu Ser Lys Leu Asn Val Lys Lys Gln His 15 10 15tta gct tat gga gct atc act tta gta gcc ctt ttt tca tgt att ttg 96Leu Ala Tyr Gly Ala Ile Thr Leu Val Ala Leu Phe Ser Cys Ile Leu

20 25 30gct?gta?acg?gtc?atc?ttt?aaa?agt?tca?caa?gtt?act?act?gaa?tct?ttg 144Ala?Val?Thr?Val?Ile?Phe?Lys?Ser?Ser?Gln?Val?Thr?Thr?Glu?Ser?Leu

35 40 45tca?aaa?gca?gat?aaa?gtt?cgc?gta?gcc?aaa?aaa?tca?aaa?atg?act?aag 192Ser?Lys?Ala?Asp?Lys?Val?Arg?Val?Ala?Lys?Lys?Ser?Lys?Met?Thr?Lys

50 55 60gcg?aca?tct?aaa?tca?aaa?gta?gaa?gat?gta?aaa?cag?gct?cca?aaa?cct 240Ala?Thr?Ser?Lys?Ser?Lys?Val?Glu?Asp?Val?Lys?Gln?Ala?Pro?Lys?Pro?65 70 75 80tct?cag?gca?tct?aat?gaa?gcc?cca?aaa?tca?agt?tct?caa?tct?aca?gaa 288ser?Gln?Ala?Ser?Asn?Glu?Ala?Pro?Lys?Ser?Ser?Ser?Gln?Ser?Thr?Glu

85 90 95gct?aat?tct?cag?caa?caa?gtt?act?gcg?agt?gaa?gag?acg?gct?gta?gaa 336Ala?Asn?Ser?Gln?Gln?Gln?Val?Thr?Ala?Ser?Glu?Glu?Thr?Ala?Val?Glu

100 105 110caa?gca?gtt?gta?aca?gaa?ata?ccc?ctg?cta?cca?gtc?agg?cac?aac?aac 384Gln?Ala?Val?Val?Thr?Glu?Ile?Pro?Leu?Leu?Pro?Val?Arg?His?Asn?Asn

115 120 125ctt?tat?gct?gtt?act?gag?aca?cct?tac?aac?cct?gct?caa?cca?cca?gac 432Leu?Tyr?Ala?Val?Thr?Glu?Thr?Pro?Tyr?Asn?Pro?Ala?Gln?Pro?Pro?Asp

130 135 140caa?gtg?gcc?agg?tat?gag?caa?tgg?aaa?tac?tgc?cag?gcg?gtc?gga?tct 480Gln?Val?Ala?Arg?Tyr?Glu?Gln?Trp?Lys?Tyr?Cys?Gln?Ala?Val?Gly?Ser145 150 155 160gct?gct?gca?gca?caa?atg?gct?gct?gca?aca?gga?gtc?cct?cag?tct?act 528Ala?Ala?Ala?Ala?Gln?Met?Ala?Ala?Ala?Thr?Gly?Val?Pro?Gln?Ser?Thr

165 170 175tgg?gaa?cat?att?att?gcc?cgt?gaa?tca?aat?ggt?aat?cct?aat?gtt?gct 576Trp?Glu?His?Ile?Ile?Ala?Arg?Glu?Ser?Asn?Gly?Asn?Pro?Asn?Val?Ala

180 185 190aat?gcc?tca?gga?gct?tca?gga?ctt?ttc?caa?acg?atg?cca?ggt?tgg?ggt 624Asn?Ala?Ser?Gly?Ala?Ser?Gly?Leu?Phe?Gln?Thr?Met?Pro?Gly?Trp?Gly

195 200 205tca?aca?gct?aca?gtt?cag?gat?caa?gta?att?cag?cta?tta?aag?ctt?att 672Ser?Thr?Ala?Thr?Val?Gln?Asp?Gln?Val?Ile?Gln?Leu?Leu?Lys?Leu?Ile

210 215 220cgt gct caa ggg tta tca gct ggg tac cag tga 705Arg Ala Gln Gly Leu Ser Ala Gly Tyr Gln225,230 235＜210〉6＜211〉234＜212〉PRT＜213〉B group streptococcus＜400〉6Met Asn Lys Arg Arg Lys Leu Ser Lys Leu Asn Val Lys Lys Gln His 15 10 15Leu Ala Tyr Gly Ala Ile Thr Leu Val Ala Leu Phe Ser Cys Ile Leu

20 25 30Ala?Val?Thr?Val?Ile?Phe?Lys?Ser?Ser?Gln?Val?Thr?Thr?Glu?Ser?Leu

35 40 45Ser?Lys?Ala?Asp?Lys?Val?Arg?Val?Ala?Lys?Lys?Ser?Lys?Met?Thr?Lys

50 55 60Ala?Thr?Ser?Lys?Ser?Lys?Val?Glu?Asp?Val?Lys?Gln?Ala?Pro?Lys?Pro?65 70 75 80Ser?Gln?Ala?Ser?Asn?Glu?Ala?Pro?Lys?Ser?Ser?Ser?Gln?Ser?Thr?Glu

85 90 95Ala?Asn?Ser?Gln?Gln?Gln?Val?Thr?Ala?Ser?Glu?Glu?Thr?Ala?Val?Glu

100 105 110

Gln?Ala?Val?Val?Thr?Glu?Ile?Pro?Leu?Leu?Pro?Val?Arg?His?Asn?Asn

115 120 125Leu?Tyr?Ala?Val?Thr?Glu?Thr?Pro?Tyr?Asn?Pro?Ala?Gln?Pro?Pro?Asp

130 135 140Gln?Val?Ala?Arg?Tyr?Glu?Gln?Trp?Lys?Tyr?Cys?Gln?Ala?Val?Gly?Ser145 150 155 160Ala?Ala?Ala?Ala?Gln?Met?Ala?Ala?Ala?Thr?Gly?Val?Pro?Gln?Ser?Thr

165 170 175Trp?Glu?His?Ile?Ile?Ala?Arg?Glu?Ser?Asn?Gly?Asn?Pro?Asn?Val?Ala

180 185 190Asn?Ala?Ser?Gly?Ala?Ser?Gly?Leu?Phe?Gln?Thr?Met?Pro?Gly?Trp?Gly

195 200 205Ser?Thr?Ala?Thr?Val?Gln?Asp?Gln?Val?Ile?Gln?Leu?Leu?Lys?Leu?Ile

210 215 220Arg Ala Gln Gly Leu Ser Ala Gly Tyr Gln225,230＜210〉7＜211〉594＜212〉DNA＜213〉B group streptococcus＜220〉＜221〉CDS＜222〉(1) .. (594)＜400〉7atg act gaa cca ttt ttt gat aaa gaa tta act tgt cgc cca att gaa 48Met Thr Glu Pro Phe Phe Asp Lys Glu Leu Thr Cys Arg Pro Ile Glu 15 10 15gcc att cct gaa ttg ttg gaa ttc gat att acc gtt cgt gga gac aac 96Ala Ile Pro Glu Leu Leu Glu Phe Asp Ile Thr Val Arg Gly Asp Asn

20 25 30cgt?gga?tgg?ttc?aaa?gag?aac?ttt?caa?aaa?gaa?aaa?atg?ata?ccg?ctt 144Arg?Gly?Trp?Phe?Lys?Glu?Asn?Phe?Gln?Lys?Glu?Lys?Met?Ile?Pro?Leu

35 40 45ggt?ttc?cca?gaa?agc?ttc?ttt?gag?gca?gac?aaa?cta?caa?aat?aat?att 192Gly?Phe?Pro?Glu?Ser?Phe?Phe?Glu?Ala?Asp?Lys?Leu?Gln?Asn?Asn?Ile

50 55 60tcg?ttt?aca?aaa?aaa?aat?act?ttg?cga?ggt?ctc?cat?gca?gag?cct?tgg 240Ser?Phe?Thr?Lys?Lys?Asn?Thr?Leu?Arg?Gly?Leu?His?Ala?Glu?Pro?Trp?65 70 75 80gat?aaa?tat?gtt?tcg?atc?gct?gat?gaa?gga?cgt?gtg?atc?ggt?act?tgg 288Asp?Lys?Tyr?Val?Ser?Ile?Ala?Asp?Glu?Gly?Arg?Val?Ile?Gly?Thr?Trp

85 90 95gtt?gac?ctc?cgt?gaa?ggt?gac?agt?ttt?ggt?aac?gtt?tac?caa?acg?att 336Val?Asp?Leu?Arg?Glu?Gly?Asp?Ser?Phe?Gly?Asn?Val?Tyr?Gln?Thr?Ile

100 105 110atc?gat?gcc?tca?aaa?ggt?att?ttt?gtt?cca?cgc?ggc?gtt?gct?aat?ggt 384Ile?Asp?Ala?Ser?Lys?Gly?Ile?Phe?Val?Pro?Arg?Gly?Val?Ala?Asn?Gly

115 120 125ttc?caa?gtt?ctt?tca?gat?aaa?gca?gct?tat?act?tat?ctc?gtt?aac?gat 432Phe?Gln?Val?Leu?Ser?Asp?Lys?Ala?Ala?Tyr?Thr?Tyr?Leu?Va1?Asn?Asp

130 135 140tat?tgg?gca?ctt?gaa?ctc?aaa?cca?aaa?tat?gct?ttc?gtt?aac?tat?gca 480Tyr?Trp?Ala?Leu?Glu?Leu?Lys?Pro?Lys?Tyr?Ala?Phe?Val?Asn?Tyr?Ala145 150 155 160gat?cca?aat?cta?ggc?att?cag?tgg?gaa?aat?ctw?gaa?gaa?gca?gaa?gtc 528Asp?Pro?Asn?Leu?Gly?Ile?Gln?Trp?Glu?Asn?Xaa?Glu?Glu?Ala?Glu?Val

165 170 175tca?gaa?gca?gat?aag?aat?cac?cca?ctt?ctc?aaa?gat?gta?aaa?cct?ttg 576Ser?Glu?Ala?Asp?Lys?Asn?His?Pro?Leu?Leu?Lys?Asp?Val?Lys?Pro?Leu

180 185 190aag?aag?gaa?gat?ttg?taa 594Lys?Lys?Glu?Asp?Leu

195＜210〉8＜211〉197＜212〉PRT＜213〉B group streptococcus＜400〉8Met Thr Glu Pro Phe Phe Asp Lys Glu Leu Thr Cys Arg Pro Ile Glu 15 10 15Ala Ile Pro Glu Leu Leu Glu Phe Asp Ile Thr Val Arg Gly Asp Asn

20 25 30Arg?Gly?Trp?Phe?Lys?Glu?Asn?Phe?Gln?Lys?Glu?Lys?Met?Ile?Pro?Leu

35 40 45Gly?Phe?Pro?Glu?Ser?Phe?Phe?Glu?Ala?Asp?Lys?Leu?Gln?Asn?Asn?Ile

50 55 60Ser?Phe?Thr?Lys?Lys?Asn?Thr?Leu?Arg?Gly?Leu?His?Ala?Glu?Pro?Trp?65 70 75 80Asp?Lys?Tyr?Val?Ser?Ile?Ala?Asp?Glu?Gly?Arg?Val?Ile?Gly?Thr?Trp

85 90 95Val?Asp?Leu?Arg?Glu?Gly?Asp?Ser?Phe?Gly?Asn?Val?Tyr?Gln?Thr?Ile

100 105 110Ile?Asp?Ala?Ser?Lys?Gly?Ile?Phe?Val?Pro?Arg?Gly?Val?Ala?Asn?Gly

115 120 125Phe?Gln?Val?Leu?Ser?Asp?Lys?Ala?Ala?Tyr?Thr?Tyr?Leu?Va1?Asn?Asp

130 135 140Tyr?Trp?Ala?Leu?Glu?Leu?Lys?Pro?Lys?Tyr?Ala?Phe?Val?Asn?Tyr?Ala145 150 155 160Asp?Pro?Asn?Leu?Gly?Ile?Gln?Trp?Glu?Asn?Xaa?Glu?Glu?Ala?Glu?Val

165 170 175Ser?Glu?Ala?Asp?Lys?Asn?His?Pro?Leu?Leu?Lys?Asp?Val?Lys?Pro?Leu

180 185 190Lys?Lys?Glu?Asp?Leu

195＜210〉9＜211〉1217＜212〉DNA＜213〉B group streptococcus＜220〉＜221〉CDS＜222〉(1) .. (570)＜220〉＜221〉CDS＜222〉(679) .. (945)＜400〉9tat tat tta arc gga ggg ttg gca gaa atg caa cat gtc aat cat tct 48Tyr Tyr Leu Ile Gly Gly Leu Ala Glu Met Gln His Val Asn His Ser 15 10 15tct ttt gat aaa gca tca aaa gca gga ttt att att gct tta ggc att 96Ser Phe Asp Lys Ala Ser Lys Ala Gly Phe Ile Ile Ala Leu Gly Ile

20 25 30 gtt?tat?gga?gat?att?ggt?aca?agc?cca?ctc?tat?acg?atg?caa?tca?ttg 144Val?Tyr?Gly?Asp?Ile?Gly?Thr?Ser?Pro?Leu?Tyr?Thr?Met?Gln?Ser?Leu

35 40 45gtt?gaa?aac?caa?ggt?ggt?att?tct?agt?gtc?aca?gaa?tcg?ttt?atc?tta 192Val?Glu?Asn?Gln?Gly?Gly?Ile?Ser?Ser?Val?Thr?Glu?Ser?Phe?Ile?Leu

50 55 60ggt?tct?ata?tct?tta?atc?ata?tgg?acc?ttg?aca?ctt?att?aca?act?atc 240Gly?Ser?Ile?Ser?Leu?Ile?Ile?Trp?Thr?Leu?Thr?Leu?Ile?Thr?Thr?Ile?65 70 75 80aag?tat?gtg?ctt?gta?gct?tta?aag?gcg?gat?aat?cac?cac?gaa?ggt?ggt 288Lys?Tyr?Val?Leu?Val?Ala?Leu?Lys?Ala?Asp?Asn?His?His?Glu?Gly?Gly

85 90 95att?ttt?tct?tta?tat?acc?ctt?gtt?aga?aaa?atg?aca?cct?tgg?tta?att 336Ile?Phe?Ser?Leu?Tyr?Thr?Leu?Val?Arg?Lys?Met?Thr?Pro?Trp?Leu?Ile

100 105 110gtt?ccg?gct?gtt?att?gga?ggt?gca?acc?ttg?ttg?tca?gat?gga?gct?ttg 384Val?Pro?Ala?Val?Ile?Gly?Gly?Ala?Thr?Leu?Leu?Ser?Asp?Gly?Ala?Leu

115 120 125acg?cca?gct?gta?acc?gta?ctt?cag?ccg?tta?agg?att?aaa?gta?gtt?cct 432Thr?Pro?Ala?Val?Thr?Val?Leu?Gln?Pro?Leu?Arg?Ile?Lys?Val?Val?Pro

130 135 140agt?ttg?cag?cat?att?tcc?aga?atc?aga?gta?tgt?tat?ttt?gcg?acc?ttg 480Ser?Leu?Gln?His?Ile?Ser?Arg?Ile?Arg?Val?Cys?Tyr?Phe?Ala?Thr?Leu145 150 155 160tta?ttt?act?gtt?act?ttt?gcc?atc?caa?ggt?ttg?gaa?cgg?gtg?tta?ttg 528Leu?Phe?Thr?Val?Thr?Phe?Ala?Ile?Gln?Gly?Leu?Glu?Arg?Val?Leu?Leu

165 170 175gaa?tta?ttg?gcc?att?atg?tta?tat?ggt?ttg?cct?ttt?ggt?tta 570Glu?Leu?Leu?Ala?Ile?Met?Leu?Tyr?Gly?Leu?Pro?Phe?Gly?Leu

180 185 190ncggtctcct?tatagttttg?cccatccaga?agttttcaag?cattaatcca?tactacggtt 630tgaaattgtt?atttagtcca?gagaatcata?aaggtatttt?tattttag?gat?cta?ttt 687

Asp?Leu?Phetcc?tgg?cga?caa?acg?gga?gca?gaa?gca?cta?tac?tct?gac?tta?ggt?cat 735Ser?Trp?Arg?Gln?Thr?Gly?Ala?Glu?Ala?Leu?Tyr?Ser?Asp?Leu?Gly?His

195 200 205gtt?ggg?cgt?gga?aat?ata?cat?gtt?tca?tgg?ccg?ttc?gtt?aag?gtt?gcc 783Val?Gly?Arg?Gly?Asn?Ile?His?Val?Ser?Trp?Pro?Phe?Val?Lys?Val?Ala210 215 220 225att?ata?ctt?tct?tat?tgt?ggg?caa?ggg?gca?tgg?att?tta?gct?aat?aag 831Ile?Ile?Leu?Ser?Tyr?Cys?Gly?Gln?Gly?Ala?Trp?Ile?Leu?Ala?Asn?Lys

230 235 240aac?gca?gga?aat?gaa?ttg?aat?ccc?ttt?ttt?gct?agt?att?cct?tcg?caa 879Asn?Ala?Gly?Asn?Glu?Leu?Asn?Pro?Phe?Phe?Ala?ser?Ile?Pro?Ser?Gln

245 250 255ttt?aca?atg?cat?gtc?gtt?att?tta?gct?act?ttg?gca?gct?atc?atc?gct 927Phe?Thr?Met?His?Val?Val?Ile?Leu?Ala?Thr?Leu?Ala?Ala?Ile?Ile?Ala

260 265 270tca?cag?gca?ctg?att?tct?ggatcaattt?accttaagtt?ctgagctatg 975Ser?Gln?Ala?Leu?Ile?Ser

275cgactaaaaa tattcccaca atttcgttca acttatcctg ttgacaatat tgggtcaaac 1035ctacatacct ggtattaatt ggttcttatt tgccattaca acctctattg gtttgctttt 1095taagacttca gcgcacatgg aagcagcata tggattagcg ataacaatta cgatgctaat 1155gacaactatt ttactgtctt tctttttaat tcaaaaagga gtaaagagag gttttagcta 1215tt 1217＜210〉10＜211〉190＜212〉PRT＜213〉B group streptococcus＜400〉10Tyr Tyr Leu Ile Gly Gly Leu Ala Glu Met Gln His Val Asn His Ser 15 10 15Ser Phe Asp Lys Ala Ser Lys Ala Gly Phe Ile Ile Ala Leu Gly Ile

20 25 30Val?Tyr?Gly?Asp?Ile?Gly?Thr?Ser?Pro?Leu?Tyr?Thr?Met?Gln?Ser?Leu

35 40 45Val?Glu?Asn?Gln?Gly?Gly?Ile?Ser?Ser?Val?Thr?Glu?Ser?Phe?Ile?Leu

50 55 60Gly?Ser?Ile?Ser?Leu?Ile?Ile?Trp?Thr?Leu?Thr?Leu?Ile?Thr?Thr?Ile?65 70 75 80Lys?Tyr?Val?Leu?Val?Ala?Leu?Lys?Ala?Asp?Asn?His?His?Glu?Gly?Gly

85 90 95Ile?Phe?Ser?Leu?Tyr?Thr?Leu?Val?Arg?Lys?Met?Thr?Pro?Trp?Leu?Ile

100 105 110Val?Pro?Ala?Val?Ile?Gly?Gly?Ala?Thr?Leu?Leu?Ser?Asp?Gly?Ala?Leu

115 120 125Thr?Pro?Ala?Val?Thr?Val?Leu?Gln?Pro?Leu?Arg?Ile?Lys?Val?Val?Pro

130 135 140Ser?Leu?Gln?His?Ile?Ser?Arg?Ile?Arg?Val?Cys?Tyr?Phe?Ala?Thr?Leu145 150 155 160Leu?Phe?Thr?Val?Thr?Phe?Ala?Ile?Gln?Gly?Leu?Glu?Arg?Val?Leu?Leu

165 170 175Glu?Leu?Leu?Ala?Ile?Met?Leu?Tyr?Gly?Leu?Pro?Phe?Gly?Leu

180 185 190＜210〉11＜211〉89＜212〉PRT＜213〉B group streptococcus＜400〉11Asp Leu Phe Ser Trp Arg Gln Thr Gly Ala Glu Ala Leu Tyr Ser Asp 15 10 15Leu Gly His Val Gly Arg Gly Asn Ile His Val Ser Trp Pro Phe Val

20 25 30Lys?Val?Ala?Ile?Ile?Leu?Ser?Tyr?Cys?Gly?Gln?Gly?Ala?Trp?Ile?Leu

35 40 45Ala?Asn?Lys?Asn?Ala?Gly?Asn?Glu?Leu?Asn?Pro?Phe?Phe?Ala?Ser?Ile

50 55 60Pro?Ser?Gln?Phe?Thr?Met?His?Val?Val?Ile?Leu?Ala?Thr?Leu?Ala?Ala?65 70 75 80Ile Ile?Ala Ser?Gln?Ala Leu Ile?Ser

85＜210〉12＜211〉378＜212〉DNA＜213〉B group streptococcus＜220〉＜221〉CDS＜222〉(1) .. (378)＜400〉12atg cag gta ttt tta aat att gtc aat aaa ttc ttt gat cca gtt att 48Met Gln Val Phe Leu Asn Ile Val Asn Lys Phe Phe Asp Pro Val Ile 15 10 15cat atg ggt tcg gga gtt gtg atg cta att gtc atg aca ggt tta gcc 96His Met Gly Ser Gly Val Val Met Leu Ile Val Met Thr Gly Leu Ala

20 25 30atg?ata?ttt?gga?gtg?aag?ttt?tct?aaa?gca?ctt?gaa?ggt?ggt?att?aag 144Met?Ile?Phe?Gly?Val?Lys Phe?Ser?LysAla?Leu?Glu?Gly?Gly?Ile?Lys

35 40 45tta?gct?att?gct?ctt?acg?ggt?att?ggt?gct?att?att?ggt?att?tta?act 192Leu?Ala?Ile?Ala?Leu?Thr?Gly?Ile?Gly?Ala?Ile?Ile?Gly?Ile?Leu?Thr

50 55 60ggt?gct?ttt?tcc?gaa?tca?ctt?caa?gct?ttt?gtt?aaa?aat?aca?gga?atc 240Gly?Ala?Phe?Ser?Glu?Ser?Leu?Gln?Ala?Phe?Val?Lys?Asn?Thr?Gly?Ile?65 70 75 80aat?cta?agc?att?att?gac?gtt?ggt?tgg?gct?cca?tta?gca?act?att?aca 288Asn?Leu?Ser?Ile?Ile?Asp?Val?Gly?Trp?Ala?Pro?Leu?Ala?Thr?Ile?Thr

85 90 95tgg?gga?tca?cca?tat?acg?ctt?tac?ttc?tta?tta?atc?atg?ctt?att?gtc 336Trp?Gly?Ser?Pro?Tyr?Thr?Leu?Tyr?Phe?Leu?Leu?Ile?Met?Leu?Ile?Val

100 105 110aat?att?gtt?atg?att?gtt?atg?aaa?aaa?aaa?cgg?ata?cct?tag 378Asn?Ile?Val?Met?Ile?Val?Met?Lys?Lys?Lys?Arg?Ile?Pro

115 120 125＜210〉13＜211〉125＜212〉PRT＜213〉B group streptococcus＜400〉13Met Gln Val Phe Leu Asn Ile Val Asn Lys Phe Phe Asp Pro Val Ile 15 10 15His Met Gly Ser Gly Val Val Met Leu Ile Val Met Thr Gly Leu Ala

20 25 30Net?Ile?Phe?Gly?Val?Lys?Phe?Ser?Lys?Ala?Leu?Glu?Gly?Gly?Ile?Lys

35 40 45Leu?Ala?Ile?Ala?Leu?Thr?Gly?Ile?Gly?Ala?Ile?Ile?Gly?Ile?Leu?Thr

50 55 60Gly?Ala?Phe?Ser?Glu?Ser?Leu?Gln?Ala?Phe?Val?Lys?Asn?Thr?Gly?Ile?65 70 75 80Asn?Leu?Ser?Ile?Ile?Asp?Val?Gly?Trp?Ala?Pro?Leu?Ala?Thr?Ile?Thr

85 90 95Trp?Gly?Ser?Pro?Tyr?Thr?Leu?Tyr?Phe?Leu?Leu?Ile?Met?Leu?Ile?Val

100 105 110Asn?Ile?Val?Met?Ile?Val?Met?Lys?Lys?Lys?Arg?Ile?Pro

115 120 125＜210〉14＜211〉705＜212〉DNA＜213〉B group streptococcus＜220〉＜221〉CDS＜222〉(118) .. (705)＜400〉14ggatcgggcg caagcttaac gattcttttt aaaatcatta aattttaaaa caaatttcag 60acatattgcc aaagttttga tattattact ataatatagt ttgtagagga gaataat 117atg ggc caa gaa cct arc atc gaa tat caa aat arc aat aaa gtg tat 165Met Gly Gln Glu Pro Ile Ile Glu Tyr Gln Asn Ile Asn Lys Val Tyr 15 10 15ggg gaa aat gtt gcg gtt gaa gat att aac ctt aaa att tac cct ggt 213Gly Glu Asn Val Ala Val Glu Asp Ile Asn Leu Lys Ile Tyr Pro Gly

20 25 30gat?ttc?gtt?tgt?ttc?atc?ggt?acg?agt?gga?tca?ggt?aaa?aca?aca?tta 261Asp?Phe?Val?Cys?Phe?Ile?Gly?Thr?Ser?Gly?Ser?Gly?Lys?Thr?Thr?Leu

35 40 45atg?cgt?atg?gtt?aac?cat?atg?tta?aaa?cca?aca?aat?ggt?act?cta?tta 309Met?Arg?Met?Val?Asn?His?Met?Leu?Lys?Pro?Thr?Asn?Gly?Thr?Leu?Leu

50 55 60ttt?aag?gga?aaa?gat?atc?tct?act?att?aac?ccc?att?gaa?tta?aga?cgc 357Phe?Lys?Gly?Lys?Asp?Ile?Ser?Thr?Ile?Asn?Pro?Ile?Glu?Leu?Arg?Arg 65 70 75 80aga?att?gga?tat?gtt?atc?caa?aac?att?ggt?tta?atg?cct?cat?atg?acc 405Arg?Ile?Gly?Tyr?Val?Ile?Gln?Asn?Ile?Gly?Leu?Met?Pro?His?Met?Thr

85 90 95att?tac?gaa?aat?ata?gtt?ctt?gta?cca?aaa?tta?ttg?aaa?tgg?tca?gaa 453Ile?Tyr?Glu?Asn?Ile?Val?Leu?Val?Pro?Lys?Leu?Leu?Lys?Trp?Ser?Glu

100 105 110gaa?gct?aaa?aga?gct?aaa?gca?agg?gaa?ctt?att?aaa?tta?gtt?gaa?tta 501Glu?Ala?Lys?Arg?Ala?Lys?Ala?Arg?Glu?Leu?Ile?Lys?Leu?Val?Glu?Leu

115 120 125ccc?gaa?gaa?tat?ttg?gat?cgc?tac?cct?agt?gag?ttg?tct?ggc?ggt?cag 549Pro?Glu?Glu?Tyr?Leu?Asp?Arg?Tyr?Pro?Ser?Glu?Leu?Ser?Gly?Gly?Gln

130 135 140caa?caa?cgt?atc?ggt?gtc?att?cgc?gct?ctt?gca?gca?gac?caa?gat?att 597Gln?Gln?Arg?Ile?Gly?Val?Ile?Arg?Ala?Leu?Ala?Ala?Asp?Gln?Asp?Ile145 150 155 160att?tta?atg?gat?gag?cct?ttt?gga?gct?ctg?gat?cct?att?act?aga?gaa 645Ile?Leu?Met?Asp?Glu?Pro?Phe?Gly?Ala?Leu?Asp?Pro?Ile?Thr?Arg?Glu

165 170 175ggt?att?caa?gac?ttt?agt?caa?gtc?tct?tca?gga?aga?aat?ggg?gga?aaa 693Gly?Ile?Gln?Asp?Phe?Ser?Gln?Val?Ser?Ser?Gly?Arg?Asn?Gly?Gly?Lys

180 185 190cta tca?tct tag 705Leu?Ser?Ser

195＜210〉15＜211〉195＜212〉PRT＜213〉B group streptococcus＜400〉15Met Gly Gln Glu Pro Ile Ile Glu Tyr Gln Asn Ile Asn Lys Val Tyr 15 10 15Gly Glu Asn Val Ala Va1 Glu Asp Ile Asn Leu Lys Ile Tyr Pro Gly

20 25 30Asp?Phe?Val?Cys?Phe?Ile?Gly?Thr?Ser?Gly?Ser?Gly?Lys?Thr?Thr?Leu

35 40 45Met?Arg?Met?Val?Asn?His?Met?Leu?Lys?Pro?Thr?Asn?Gly?Thr?Leu?Leu

50 55 60Phe?Lys?Gly?Lys?Asp?Ile?Ser?Thr?Ile?Asn?Pro?Ile?Glu?Leu?Arg?Arg?65 70 75 80Arg?Ile?Gly?Tyr?Val?Ile?Gln?Asn?Ile?Gly?Leu?Met?Pro?His?Met?Thr

85 90 95Ile?Tyr?Glu?Asn?Ile?Val?Leu?Val?Pro?Lys?Leu?Leu?Lys?Trp?Ser?Glu

100 105 110Glu?Ala?Lys?Arg?Ala?Lys?Ala?Arg?Glu?Leu?Ile?Lys?Leu?Val?Glu?Leu

115 120 125Pro?Glu?Glu?Tyr?Leu?Asp?Arg?Tyr?Pro?Ser?Glu?Leu?Ser?Gly?Gly?Gln

130 135 140Gln?Gln?Arg?Ile?Gly?Val?Ile?Arg?Ala?Leu?Ala?Ala?Asp?Gln?Asp?Ile145 150 155 160Ile?Leu?Met?Asp?Glu?Pro?Phe?Gly?Ala?Leu?Asp?Pro?Ile?Thr?Arg?Glu

165 170 175Gly?Ile?Gln?Asp?Phe?Ser?Gln?Val?Ser?Ser?Gly?Arg?Asn?Gly?Gly?Lys

180 185 190Leu?Ser?Ser

195＜210〉16＜211〉367＜212〉DNA＜213〉B group streptococcus＜220〉＜221〉CDS＜222〉(1) .. (366)＜400〉16atc cct tat agt gat gtt ttt gct aca gga gga ttt tta tac tat gta 48Ile Pro Tyr Ser Asp Val Phe Ala Thr Gly Gly Phe Leu Tyr Tyr Val 15 10 15acg att gct cta agt tac ctt tta ggg tct agt atc tgg tta ttt att 96Thr IleA la Leu Ser Tyr Leu Leu Gly Ser Ser Ile Trp Leu Phe Ile

20 25 30gta?cag?ttt?att?gct?tac?tat?gta?tct?gga?att?tat?ttt?tat?aaa?tta 144Val?Gln?Phe?Ile?Ala?Tyr?Tyr?Val?Ser?Gly?Ile?Tyr?Phe?Tyr?Lys?Leu

35 40 45gtt?tat?tat?gtg?gca?caa?agt?gaa?att?gtc?tcg?ata?ggc?atg?acg?ttg 192Val?Tyr?Tyr?Val?Ala?Gln?Ser?Glu?Ile?Val?Ser?Ile?Gly?Met?Thr?Leu

50 55 60att?ttc?tat?ata?atg?aat?att?gtc?tta?gga?ttc?ggt?ggt?atg?tac?cca 240Ile?Phe?Tyr?Ile?Met?Asn?Ile?Val?Leu?Gly?Phe?Gly?Gly?Met?Tyr?Pro?65 70 75 80ara?cag?tgg?gca?tta?cct?ttt?atg?crc?att?tcg?cta?tgg?ttt?tta?att 288Ile?Gln?Trp?Ala?Leu?Pro?Phe?Met?Leu?Ile?Ser?Leu?Trp?Phe?Leu?Ile

85 90 95aaa?ttt?tgt?gtc?gat?aat?atc?gtt?gat?gaa?gca?ttt?ata?ttt?tat?ggt 336Lys?Phe?cys?Val?Asp?Asn?Ile?Val?Asp?Glu?Ala?Phe?Ile?Phe?Tyr?Gly

100 105 110att?tta?gca?gca?ttc?tca?cta?ttt?ata?gat?c 367Ile?Leu?Ala?Ala?Phe?Ser?Leu?Phe?Ile?Asp

115 120＜210〉17＜211〉122＜212〉PRT＜213〉B group streptococcus＜400〉17Ile Pro Tyr Ser Asp Val Phe Ala Thr Gly Gly Phe Leu Tyr Tyr Val 15 10 15Thr Ile Ala Leu Ser Tyr Leu Leu Gly Ser Ser Ile Trp Leu Phe Ile

20 25 30Val?Gln?Phe?Ile?Ala?Tyr?Tyr?Val?Ser?Gly?Ile?Tyr?Phe?Tyr?Lys?Leu

35 40 45Val?Tyr?Tyr?Val?Ala?Gln?Ser?Glu?Ile?Val?Ser?Ile?Gly?Met?Thr?Leu

50 55 60Ile?Phe?Tyr?Ile?Met?Asn?Ile?Val?Leu?Gly?Phe?Gly?Gly?Met?Tyr?Pro?65 70 75 80Ile?Gln?Trp?Ala?Leu?Pro?Phe?Net?Leu?Ile?Ser?Leu?Trp?Phe?Leu?Ile

85 90 95Lys?Phe?Cys?Val?Asp?Asn?Ile?Val?Asp?Glu?Ala?Phe?Ile?Phe?Tyr?Gly

100 105 110Ile?Leu?Ala?Ala?Phe?Ser?Leu Phe?Ile?Asp

115 120＜210〉18＜211〉570＜212〉DNA＜213〉B group streptococcus＜220〉＜221〉CDS＜222〉(1) .. (570)＜400〉18atg agg aaa cgt ttt tcc ttg cta aat ttt att gtt gtt act ttt att 48Met Arg Lys Arg Phe Ser Leu Leu Asn Phe Ile Val Val Thr Phe Ile 15 10 15ttc ttt ttc ttt att ctt ttt ccg ctt tta aac cat aag gga aaa gta 96Phe Phe Phe Phe Ile Leu Phe Pro Leu Leu Asn His Lys Gly Lys Val

20 25 30gat?gct?aat?tct?agg?cag?agt?gtt?acc?tac?acc?aaa?gaa?gaa?ttt?ata 144Asp?Ala?Asn?Ser?Arg?Gln?Ser?Val?Thr?Tyr?Thr?Lys?Glu?Glu?Phe?Ile

35 40 45caa?aaa?att?gtg?cca?gat?gcg?caa?gat?cta?gga?aag?tcg?tac?ggt?att 192Gln?Lys?Ile?Val?Pro?Asp?Ala?Gln?Asp?Leu?Gly?Lys?Ser?Tyr?Gly?Ile

50 55 60cgt?cct?tca?ttt?att?att?gca?cag?gcg?gct?ttg?gat?tct?gat?ttc?gga 240Arg?Pro?Ser?Phe?Ile?Ile?Ala?Gln?Ala?Ala?Leu?Asp?Ser?Asp?Phe?Gly?65 70 75 80gag?aaa?tat?agc?tat?agt?atc?ata?atc?tgt?tgg?ttg?ctt?gca?gaa?cca 288Glu?Lys?Tyr?Ser?Tyr?Ser?Ile?Ile?Ile?Cys?Trp?Leu?Leu?Ala?Glu?Pro

85 90 95gga?acg?ccc?tca?att?acc?tta?aat?gat?agt?agt?aca?gga?aaa?aaa?cag 336Gly?Thr?Pro?Ser?Ile?Thr?Leu?Asn?Asp?Ser?Ser?Thr?Gly?Lys?Lys?Gln

100 105 110gaa?aag?caa?ttt?act?cat?tat?aaa?tct?tgg?aag?tat?tca?atg?gat?gat 384Glu?Lys?Gln?Phe?Thr?His?Tyr?Lys?Ser?Trp?Lys?Tyr?Ser?Met?Asp?Asp

115 120 125tac?ctt?gct?cat?ata?aaa?tct?gga?gcg?aca?ggc?aaa?aaa?gat?tca?tat 432Tyr?Leu?Ala?His?Ile?Lys?Ser?Gly?Ala?Thr?Gly?Lys?Lys?Asp?Ser?Tyr

130 135 140act?ata?atg?gtg?tct?gtt?aaa?aat?cca?aaa?act?tta?gtg?caa?aaa?tta 480Thr?Ile?Met?Val?Ser?Val?Lys?Asn?Pro?Lys?Thr?Leu?Val?Gln?Lys?Leu145 150 155 160caa?gat?agt?ggt?ttt?gat?aat?gac?aaa?aag?tac?gct?aaa?aaa?atg?acg 528Gln?Asp?Ser?Gly?Phe?Asp?Asn?Asp?Lys?Lys?Tyr?Ala?Lys?Lys?Met?Thr

165 170 175gaa?atc?att?gat?ttg?tat?gat?tta?aca?aga?tat?gat?aag?tga 570Glu?Ile?Ile?Asp?Leu?Tyr?Asp?Leu?Thr?Arg?Tyr?Asp?Lys

180 185 190＜210〉19＜211〉189＜212〉PRT＜213〉B group streptococcus＜400〉19Met Arg Lys Arg Phe ser Leu Leu Asn Phe Ile Val Val Thr Phe Ile 15 10 15Phe Phe Phe Phe Ile Leu Phe Pro Leu Leu Asn His Lys Gly Lys Val

20 25 30Asp?Ala?Asn?Ser?Arg?Gln?Ser?Val?Thr?Tyr?Thr?Lys?Glu?Glu?Phe?Ile

35 40 45Gln?Lys?Ile?Val?Pro?Asp?Ala?Gln?Asp?Leu?Gly?Lys?Ser?Tyr?Gly?Ile

50 55 60Arg?Pro?Ser?Phe?Ile?Ile?Ala?Gln?Ala?Ala?Leu?Asp?Ser?Asp?Phe?Gly?65 70 75 80Glu?Lys?Tyr?Ser?Tyr?Ser?Ile?Ile?Ile?Cys?Trp?Leu?Leu?Ala?Glu?Pro

85 90 95Gly?Thr?Pro?Ser?Ile?Thr?Leu?Asn?Asp?Ser?Ser?Thr?Gly?Lys?Lys?Gln

100 105 110Glu?Lys?Gln?Phe?Thr?His?Tyr?Lys?Ser?Trp?Lys?Tyr?Ser?Met?Asp?Asp

115 120 125Tyr?Leu?Ala?His?Ile?Lys?Ser?Gly?Ala?Thr?Gly?Lys?Lys?Asp?Ser?Tyr

130 135 140Thr?Ile?Met?Val?Ser?Val?Lys?Asn?Pro?Lys?Thr?Leu?Val?Gln?Lys?Leu145 150 155 160Gln?Asp?Ser?Gly?Phe?Asp?Asn?Asp?Lys?Lys?Tyr?Ala?Lys?Lys?Met?Thr

165 170 175Glu?Ile?Ile?Asp?Leu?Tyr?Asp?Leu?Thr?Arg?TyrAsp?Lys

180 185＜210〉20＜211〉978＜212〉DNA＜213〉B group streptococcus＜220〉＜221〉CDS＜222〉(1) .. (978)＜400〉20atg ctt gtc atc att ttg atc att gta cta gct agt ctg aca gtg acg 48Met Leu Val Ile Ile Leu Ile Ile Val Leu Ala Ser Leu Thr Val Thr 15 10 15ata att tct tac cca aaa atg acg gaa tta aca aag tcc gtt gaa aaa 96Ile Ile Ser Tyr Pro Lys Met Thr Glu Leu Thr Lys Ser Val Glu Lys

20 25 30caa?ctt?gaa?gat?aat?gct?gat?aat?cta?tca?gac?caa?ctg?aca?tat?cag 144Gln?Leu?Glu?Asp?Asn?Ala?Asp?Asn?Leu?Ser?Asp?Gln?Leu?Thr?Tyr?Gln

35 40 45ata?gaa?gtg?gcg?caa?aaa?gat?caa?atc?tac?gtg?act?aat?cag?cta?aac 192Ile?Glu?Val?Ala?Gln?Lys?Asp?Gln?Ile?Tyr?Val?Thr?Asn?Gln?Leu?Asn

50 55 60cgt?atg?caa?cag?gaa?att?atc?agt?cgc?tta?ccg?ata?tgc?gta?cag?aat 240Arg?Met?Gln?Gln?Glu?Ile?Ile?Ser?Arg?Leu?Pro?Ile?Cys?Val?Gln?Asn 65 70 75 80aaa?tca?gca?tta?acg?gag?agt?cga?gat?cga?tca?gac?aaa?cgc?ttg?gaa 288Lys?Ser?Ala?Leu?Thr?Glu?Ser?Arg?Asp?Arg?Ser?Asp?Lys?Arg?Leu?Glu

85 90 95ttg?att?aac?tcc?aat?tta?tct?cag?tca?gtt?cag?aaa?atg?caa?gat?tca 336Leu?Ile?Asn?Ser?Asn?Leu?Ser?Gln?Ser?Val?Gln?Lys?Met?Gln?Asp?Ser

100 105 110atg?aaa?aac?gct?tgg?atc?aaa?tgc?gcc?aaa?ctg?ttg?agg?aaa?agc?tgg 384Met?Lys?Asn?Ala?Trp?Ile?Lys?Cys?Ala?Lys?Leu?Leu?Arg?Lys?Ser?Trp

115 120 125aaa?aaa?cgc?tac?aaa?cgc?gtt?gca?aac?ttc?ttt?gaa?act?gta?tcg?cgt 432Lys?Lys?Arg?Tyr?Lys?Arg?Val?Ala?Asn?Phe?Phe?Glu?Thr?Val?Ser?Arg

130 135 140caa?cta?gag?agc?gtc?aat?caa?ggt?ctg?ggt?aga?tgg?aaa?ctg?tgc?caa 480Gln?Leu?Glu?Ser?Val?Asn?Gln?Gly?Leu?Gly?Arg?Trp?Lys?Leu?cys?Gln145 150 155 160gat?gtt?ggt?acc?act?gaa?caa?agt?ctg?tca?aat?act?aag?aca?agg?gga 528Asp?Val?Gly?Thr?Thr?Glu?Gln?Ser?Leu?Ser?Asn?Thr?Lys?Thr?Arg?Gly

165 170 175ata?tta?ggg?gag?tta?caa?ctc?ggt?caa?att?ata?gaa?gat?att?atg?aca 576Ile?Leu?Gly?Glu?Leu?Gln?Leu?Gly?Gln?Ile?Ile?Glu?Asp?Ile?Met?Thr

180 185 190gtt?agt?caa?tat?gag?aga?gaa?ttt?cct?acg?gtg?tct?ggc?tct?tct?gag 624Val?Ser?Gln?Tyr?Glu?Arg?Glu?Phe?Pro?Thr?Val?Ser?Gly?Ser?Ser?Glu

195 200 205cgt?gtt?gaa?tat?gct?att?aaa?tac?ctg?gaa?atg?gtc?agg?gag?att?ata 672Arg?Val?Glu?Tyr?Ala?Ile?Lys?Tyr?Leu?Glu?Met?Val?Arg?Glu?Ile?Ile

210 215 220tct?att?tgc?cta?ttg?act?cta?agt?ttc?tct?aga?aga?tta?tta?ccg?att 720Ser?Ile?Cys?Leu?Leu?Thr?Leu?Ser?Phe?Ser?Arg?Arg?Leu?Leu?Pro?Ile225 230 235 240ggg?aga?tgc?tta?tgg?aat?tgg?gtg?acc?agg?ttc?aaa?tgg?aac?tct?att 768Gly?Arg?Cys?Leu?Trp?Asn?Trp?Val?Thr?Arg?Phe?Lys?Trp?Asn?Ser?Ile

245 250 255cgt?aat?ctt?tac?tgg?gga?agt?att?cgt?aaa?ttt?gca?aaa?gat?ata?aac 816Arg?Asn?Leu?Tyr?Trp?Ala?Ser?Ile?Arg?Lys?Phe?Ala?Lys?Asp?Ile?Asn

260 265 270aat?aag?tac?tta?aat?cct?cct?gaa?acg?aca?aat?ttt?ggt?atc?atg?ttc 864Asn?Lys?Tyr?Leu?Asn?Pro?Pro?Glu?Thr?Thr?Asn?Phe?Gly?Ile?Met?Phe

275 280 285tta?cca?act?gaa?ggg?ctc?tat?tct?gaa?gtg?gta?aga?aat?gca?aca?ttc 912Leu?Pro?Thr?Glu?Gly?Leu?Tyr?Ser?Glu?Val?Val?Arg?Asn?Ala?Thr?Phe

290 295 300ttt?gat?agt?cta?aga?cgt?gac?gaa?aat?att?gta?gta?gct?gga?ccg?tca 960Phe?Asp?Ser?Leu?Arg?Arg?Asp?Glu?Asn?Ile?Val?Val?Ala?Gly?Pro?Ser305 310 315 320acc?tta?tct?gct tac?taa 978Thr?Leu?SerA?la?Tyr

325＜210〉21＜211〉325＜212〉PRT＜213〉B group streptococcus＜400〉21Met Leu Val Ile Ile Leu Ile Ile Val Leu Ala Ser Leu Thr Val Thr 15 10 15Ile Ile Ser Tyr Pro Lys Met Thr Glu Leu Thr Lys Ser Val Glu Lys

20 25 30Gln?Leu?Glu?Asp?Asn?Ala?Asp?Asn?Leu?Ser?Asp?Gln?Leu?Thr?Tyr?Gln

35 40 45Ile?Glu?Val?Ala?Gln?Lys?Asp?Gln?Ile?Tyr?Val?Thr?Asn?Gln?Leu?Asn

50 55 60Arg?Met?Gln?Gln?Glu?Ile?Ile?Ser?Arg?Leu?Pro?Ile?Cys?Val?Gln?Asn?65 70 75 80Lys?Ser?Ala?Leu?Thr?Glu?Ser?Arg?Asp?Arg?Ser?Asp?Lys?Arg?Leu?Glu

85 90 95Leu?Ile?Asn?Ser?Asn?Leu?Ser?Gln?Ser?Val?Gln?Lys?Met?Gln?Asp?Ser

100 105 110Met?Lys?Asn?Ala?Trp?Ile?Lys?Cys?Ala?Lys?Leu?Leu?Arg?Lys?Ser?Trp

115 120 125 Lys?Lys?Arg?Tyr?Lys?Arg?Val?Ala?Asn?Phe?Phe?Glu?Thr?Val?Ser?Arg

130 135 140Gln?Leu?Glu?Ser?Val?Asn?Gln?Gly?Leu?Gly?Arg?Trp?Lys?Leu?Cys?Gln145 150 155 160Asp?Val?Gly?Thr?Thr?Glu?Gln?Ser?Leu?Ser?Asn?Thr?Lys?Thr?Arg?Gly

165 170 175Ile?Leu?Gly?Glu?Leu?Gln?Leu?Gly?Gln?Ile?Ile?Glu?Asp?Ile?Met?Thr

180 185 190Val?Ser?Gln?Tyr?Glu?Arg?Glu?Phe?Pro?Thr?Val?Ser?Gly?Ser?Ser?Glu

195 200 205Arg?Val?Glu?Tyr?Ala?Ile?Lys?Tyr?Leu?Glu?Met?Val?Arg?Glu?Ile?Ile

210 215 220Ser?Ile?Cys?Leu?Leu?Thr?Leu?Ser?Phe?Ser?Arg?Arg?Leu?Leu?Pro?Ile225 230 235 240Gly?Arg?Cys?Leu?Trp?Asn?Trp?Val?Thr?Arg?Phe?Lys?Trp?Asn?Ser?Ile

245 250 255Arg?Asn?Leu?Tyr?Trp?Ala?Ser?Ile?Arg?Lys?Phe?Ala?Lys?Asp?Ile?Asn

260 265 270Asn?Lys?Tyr?Leu?Asn?Pro?Pro?Glu?Thr?Thr?Asn?Phe?Gly?Ile?Met?Phe

275 280 285Leu?Pro?Thr?Glu?Gly?Leu?Tyr?Ser?Glu?Val?Val?Arg?Asn?Ala?Thr?Phe

290 295 300Phe?Asp?Ser?Leu?Arg?Arg?Asp?Glu?Asn?Ile?Val?Val?Ala?Gly?Pro?Ser305 310 315 320Thr?Leu?Ser?Ala?Tyr

325＜210〉22＜211〉579＜212〉DNA＜213〉B group streptococcus＜220〉＜221〉CDS＜222〉(1) .. (579)＜400〉22atg cga aaa gaa gtg aca cca gag atg ctt aac tat aat aag tat cct 48Met Arg Lys Glu Val Thr Pro Glu Met Leu Asn Tyr Asn Lys Tyr Pro 15 10 15ggc cca cag ttt att cac ttt gaa aat atc gtt aaa agt gat gat att 96Gly Pro Gln Phe Ile His Phe Glu Asn Ile Val Lys Ser Asp Asp Ile

20 25 30gaa?ttt?caa?ctt?gtt?att?aat?gaa?aaa?tca?gct?ttt?gat?gtg?act?gtc 144Glu?Phe?Gln?Leu?Val?Ile?Asn?Glu?Lys?Ser?Ala?Phe?Asp?Val?Thr?Val

35 40 45ttt?gga?caa?cgt?ttt?tct?gag?att?tta?tta?aaa?tat?gat?ttt?atc?gtt 192Phe?Gly?Gln?Arg?Phe?Ser?Glu?Ile?Leu?Leu?Lys?Tyr?Asp?Phe?Ile?Val

50 55 60ggc?gat?tgg?ggt?aac?gag?cag?ttg?agg?cta?aga?ggc?ttt?tac?aaa?gat 240Gly?Asp?Trp?Gly?Asn?Glu?Gln?Leu?Arg?Leu?Arg?Gly?Phe?Tyr?Lys?Asp?65 70 75 80gct?agt?acg?att?aga?aaa?aat?agc?cgg?att?tca?cgt?tta?gaa?gat?tat 288Ala?Ser?Thr?Ile?Arg?Lys?Asn?Ser?Arg?Ile?Ser?Arg?Leu?Glu?Asp?Tyr

85 90 95att?aaa?gag?tat?tgt?aac?ttt?ggt?tgt?gct?tat?ttt?gtg?ttg?gag?aat 336Ile?Lys?Glu?Tyr?Cys?Asn?Phe?Gly?Cys?Ala?Tyr?Phe?Val?Leu?Glu?Asn

100 105 110cca?aat?cct?aga?gat?att?aaa?ttt?gat?gat?gaa?aga?cct?cat?aag?cgt 384Pro?Asn?Pro?Arg?Asp?Ile?Lys?Phe?Asp?Asp?Glu?Arg?Pro?His?Lys?Arg

115 120 125cgt?aag?tca?aga?tcc?aaa?tca?caa?tca?tca?aag?tca?caa?act?aga?aat 432Arg?Lys?ser?Arg?Ser?Lys?Ser?Gln?Ser?Ser?Lys?Ser?Gln?Thr?Arg?Asn

130 135 140aat?cgt?tcc?cag?tca?aat?gcc?aat?gct?cat?ttt?aca?agt?aaa?aag?cgt 480Asn?Arg?Ser?Gln?Ser?Asn?Ala?Asn?Ala?His?Phe?Thr?Ser?Lys?Lys?Arg145 150 155 160aaa?gac?aca?aaa?cgc?cgt?caa?gaa?cgt?cat?att?aaa?gaa?gag?caa?gat 528Lys?Asp?Thr?Lys?Arg?Arg?Gln?Glu?Arg?His?Ile?Lys?Glu?Glu?Gln?Asp

165 170 175 aag?gaa?atg?acc?tct?gca?aag?cag?cat?ttg?tta?ttc?gta?aga?aaa?aat 576Lys?Glu?Met?Thr?Ser?Ala?Lys?Gln?His?Leu?Leu?Phe?Val?Arg?Lys?Asn

180 185 190taa 579＜210〉23＜21l〉192＜212〉PRT＜213〉B group streptococcus＜400〉23Met Arg Lys Glu Val Thr Pro Glu Met Leu Asn Tyr Asn Lys Tyr Pro 15 10 15Gly Pro Gln Phe Ile His Phe Glu Asn Ile Val Lys Ser Asp Asp Ile

20 25 30Glu?Phe?Gln?Leu?Val?Ile?Asn?Glu?Lys?SerA?la?Phe?Asp?Val?Thr?Val

35 40 45Phe?Gly?Gln?Arg?Phe?Ser?Glu?Ile?Leu?Leu?Lys?Tyr?Asp?Phe?Ile?Val

50 55 60Gly?Asp?Trp?Gly?Asn?Glu?Gln?Leu?Arg?Leu?Arg?Gly?Phe?Tyr?Lys?Asp?65 70 75 80Ala?Ser?Thr?Ile?Arg?Lys?Asn?Ser?Arg?Ile?Ser?Arg?Leu?Glu?Asp?Tyr

85 90 95Ile?Lys?Glu?Tyr?Cys?Asn?Phe?Gly?Cys?Ala?Tyr?Phe?Val?Leu?Glu?Asn

100 105 110Pro?Asn?Pro?Arg?Asp?Ile?Lys?Phe?Asp?Asp?Glu?Arg?Pro?His?Lys?Arg

115 120 125Arg?Lys?Ser?Arg?Ser?Lys?Ser?Gln?Ser?Ser?Lys?Ser?Gln?Thr?Arg?Asn

130 135 140Asn?Arg?Ser?Gln?Ser?Asn?Ala?Asn?Ala?His?Phe?Thr?Ser?Lys?Lys?Arg145 150 155 160Lys?Asp?Thr?Lys?Arg?Arg?Gln?Glu?Arg?His?Ile?Lys?Glu?Glu?Gln?Asp

165 170 175Lys?Glu?Met?Thr?Ser?Ala?Lys?Gln?His?Leu?Leu?Phe?Val?Arg?Lys?Asn

180 185 190＜210〉24＜211〉609＜212〉DNA＜213〉B group streptococcus＜220〉＜221〉CDS＜222〉(1) .. (609)＜400〉24atg aca ata aaa aaa gtg tta agt gta aca gga att att tta gtg aca 48Met Thr Ile Lys Lys Val Leu Ser Val Thr Gly Ile Ile Leu Val Thr 15 10 15gta gcg tct cta gct gct tgt agc tca aaa tct cat act act aag acg 96Val Ala Ser Leu Ala Ala Cys Ser Ser Lys Ser His Thr Thr Lys Thr

20 25 30ggc?aaa?aaa?gaa?gtt?aat?ttt?gca?act?gtt?gga?aca?acg?gca?cct?ttt 144Gly?Lys?Lys?Glu?Val?Asn?Phe?Ala?Thr?Val?Gly?Thr?Thr?Ala?Pro?Phe

35 40 45tct?tat?gtg?aag?gat?ggg?aaa?ctg?act?ggc?ttt?gat?att?gaa?gta?gcc 192Ser?Tyr?Val?Lys?Asp?Gly?Lys?Leu?Thr?Gly?Phe?Asp?Ile?Glu?Val?Ala

50 55 60aaa?gct?gtt?ttt?aaa?ggt?tca?gat?aac?tat?aaa?gtc?act?ttt?aaa?aaa 240Lys?Ala?Val?Phe?Lys?Gly?Ser?Asp?Asn?Tyr?Lys?Val?Thr?Phe?Lys?Lys?65 70 75 80aca?gaa?tgg?tca?tcg?gta?ttt?acc?ggc?att?gat?tca?gga?aag?ttt?caa 288Thr?Glu?Trp?Ser?Ser?Val?Phe?Thr?Gly?Ile?Asp?Ser?Gly?Lys?Phe?Gln

85 90 95atg?ggt?gga?aat?aat?att?tct?tat?rca?tca?gag?aga?tct?caa?aaa?tay 336Met?Gly?Gly?Asn?Asn?Ile?Ser?Tyr?Ser?Ser?Glu?Arg?Ser?Gln?Lys?Tyr

100 105 110tta?ttt?tca?tac?cca?ata?ggc?tct?act?cct?tca?gtt?tta?gca?gtt?cct 384Leu?Phe?Ser?Tyr?Pro?Ile?Gly?Ser?Thr?Pro?Ser?Val?Leu?Ala?Val?Pro

115 120 125aag?aat?agt?aat?atc?aaa?gct?tat?aat?gat?att?agt?ggt?cat?aaa?aca 432Lys?Asn?Ser?Asn?Ile?Lys?Ala?Tyr?Asn?Asp?Ile?Ser?Gly?His?Lys?Thr

130 135 140cag?gtt?gtc?caa?gga?acg?aca?act?gcc?aag?caa?tta?gaa?aat?ttc?aat 480Gln?Val?Val?Gln?Gly?Thr?Thr?Thr?Ala?Lys?Gln?Leu?Glu?Asn?Phe?Asn145 150 155 160aaa?gag?cat?cag?aaa?aat?cct?gtt?act?cta?aaa?tat?act?aat?gaa?aat 528Lys?Glu?His?Gln?Lys?Asn?Pro?Val?Thr?Leu?Lys?Tyr?Thr?Asn?Glu?Asn

165 170 175att?aca?cag?att?cta?acg?aat?ttg?agt?gat?gga?aaa?gct?gat?ttt?aaa 576Ile?Thr?Gln?Ile?Leu?Thr?Asn?Leu?Ser?Asp?Gly?Lys?Ala?Asp?Phe?Lys

180 185 190ctt?ttg?acg?gac?caa?ctg?tta?acg?cta?tta?taa 609Leu?Leu?Thr?Asp?Gln?Leu?Leu?Thr?Leu?Leu

195 200＜210〉25＜211〉202＜212〉PRT＜213〉B group streptococcus＜400〉25Met Thr Ile Lys Lys Val Leu Ser Val Thr Gly Ile Ile Leu Val Thr 15 10 15Val Ala Ser Leu Ala Ala Cys Ser Ser Lys Ser His Thr Thr Lys Thr

20 25 30Gly?Lys?Lys?Glu?Val?Asn?Phe?Ala?Thr?Val?Gly?Thr?Thr?Ala?Pro?Phe

35 40 45Ser?Tyr?Val?Lys?Asp?Gly?Lys?Leu?Thr?Gly?Phe?Asp?Ile?Glu?Val?Ala

50 55 60Lys?Ala?Val?Phe?Lys?Gly?Ser?Asp?Asn?Tyr?Lys?Val?Thr?Phe?Lys?Lys?65 70 75 80Thr?Glu?Trp?Ser?Ser?Val?Phe?Thr?Gly?Ile?Asp?Ser?Gly?Lys?Phe?Gln

85 90 95Met?Gly?Gly?Asn?Asn?Ile?Ser?Tyr?Ser?Ser?Glu?Arg?Ser?Gln?Lys?Tyr

100 105 110Leu?Phe?Ser?Tyr?Pro?Ile?Gly?Ser?Thr?Pro?Ser?Val?Leu?Ala?Val?Pro

115 120 125Lys?Asn?Ser?Asn?Ile?Lys?Ala?Tyr?Asn?Asp?Ile?Ser?Gly?His?Lys?Thr

130 135 140Gln?Val?Val?Gln?Gly?Thr?Thr?Thr?Ala?Lys?Gln?Leu?Glu?Asn?Phe?Asn145 150 155 160Lys?Glu?His?Gln?Lys?Asn?Pro?Val?Thr?Leu?Lys?Tyr?Thr?Asn?Glu?Asn

165 170 175Ile?Thr?Gln?Ile?Leu?Thr?Asn?Leu?Ser?Asp?Gly?Lys?Ala?Asp?Phe?Lys

180 185 190Leu?Leu?Thr?Asp?Gln?Leu?Leu?Thr?Leu?Leu

195 200＜210〉26＜211〉357＜212〉DNA＜213〉B group streptococcus＜220〉＜221〉CDS＜222〉(1) .. (357)＜400〉26atg aag aat ata aca aag cta tca act gtt gct tta agc cta cta ctt 48Met Lys Asn Ile Thr Lys Leu Ser Thr Val Ala Leu Ser Leu Leu Leu 15 10 15tgt acg gcg tgt gct gca tca aac acg tct aca tct aaa aca cag tct 96Cys Thr Ala Cys Ala Ala Ser Asn Thr Ser Thr Ser Lys Thr Gln Ser

20 25 30cat?cat?cct?aaa?caa?act?aaa?ctc?aca?gat?aag?caa?aaa?gaa?gaa?ccc 144His?His?Pro?Lys?Gln?Thr?Lys?Leu?Thr?Asp?Lys?Gln?Lys?Glu?Glu?Pro

35 40 45aaa?aac?aaa?gaa?gct?gct?gat?caa?gag?atg?cat?ccc?caa?ggc?gct?gtt 192Lys?Asn?Lys?Glu?Ala?Ala?Asp?Gln?Glu?Met?His?Pro?Gln?Gly?Ala?Val

50 55 60gat?ttg?aca?aaa?tat?aag?gca?aaa?ccg?gtc?aaa?gat?tat?gga?aaa?aaa 240Asp?Leu?Thr?Lys?Tyr?Lys?Ala?Lys?Pro?Val?Lys?Asp?Tyr?Gly?Lys?Lys?65 70 75 80atc?gat?gtt?ggt?gat?ggc?aag?aaa?atg?aac?att?tat?gaa?act?ggt?cag 288Ile?Asp?Val?Gly?Asp?Gly?Lys?Lys?Met?Asn?Ile?Tyr?Glu?Thr?Gly?Gln

85 90 95gga?aaa?att?cca?att?gtt?ttt?att?cct?ggt?caa?gct?gag?att?cgc?cac 336Gly?Lys?Ile?Pro?Ile?Val?Phe?Ile?Pro?Gly?Gln?Ala?Glu?Ile?Arg?His

100 105 110gct?atg?ctt?ata?aga?att?taa 357Ala?Met?Leu?Ile?Arg?Ile

115＜210〉27＜211〉118＜212〉PRT＜213〉B group streptococcus＜400〉27Met LysAsn Ile Thr Lys Leu Ser Thr Val Ala Leu Ser Leu Leu Leu 15 10 15Cys ThrAla Cys Ala Ala Ser Asn Thr Ser Thr Ser Lys Thr Gln Ser

20 25 30His?His?Pro?Lys?Gln?Thr?Lys?Leu?Thr?Asp?Lys?Gln?Lys?Glu?Glu?Pro

35 40 45Lys?Asn?Lys?Glu?Ala?Ala?Asp?Gln?Glu?Met?His?Pro?Gln?Gly?Ala?Val

50 55 60Asp?Leu?Thr?Lys?Tyr?Lys?Ala?Lys?Pro?Val?Lys?Asp?Tyr?Gly?Lys?Lys?65 70 75 80Ile?Asp?Val?Gly?Asp?Gly?Lys?Lys?Met?Asn?Ile?Tyr?Glu?Thr?Gly?Gln

85 90 95Gly?Lys?Ile?Pro?Ile?Val?Phe?Ile?Pro?Gly?Gln?Ala?Glu?Ile?Arg?His

100 105 110Ala?Met?Leu?Ile?Arg?Ile

115＜210〉28＜211〉1191＜212〉DNA＜213〉B group streptococcus＜220〉＜221〉CDS＜222〉(1) .. (1191)＜400〉28gtg aat gaa tcg acc atc aga aaa gaa ttt aaa ata gtt gtt ttt aaa 48Val Asn Glu Ser Thr Ile Arg Lys Glu Phe Lys Ile Val Val Phe Lys 15 10 15tgg atc tta aat aat caa gca gtt att gct ctc atg att acc ttt ttg 96Trp Ile Leu Asn Asn Gln Ala Val Ile Ala Leu Met Ile Thr Phe Leu

20 25 30gta?ttt?tta?acg?att?ttt?att?ttt?acc?aaa?atc?tct?ttt?atg?ttt?aaa 144Val?Phe?Leu?Thr?Ile?Phe?Ile?Phe?Thr?Lys?Ile?Ser?Phe?Met?Phe?Lys

35 40 45cct?gtg?ttt?gat?ttt?ctt?gct?gtg?ctg?ata?ttg?ccg?ctt?gta?att?tct 192Pro?Val?Phe?Asp?Phe?Leu?Ala?Val?Leu?Ile?Leu?Pro?Leu?Val?Ile?Ser

50 55 60ggc?ttg?ctt?tat?tac?cta?tta?aaa?cct?atg?gtt?aca?ttt?tta?gag?aag 240Gly?Leu?Leu?Tyr?Tyr?Leu?Leu?Lys?Pro?Met?Val?Thr?Phe?Leu?Glu?Lys?65 70 75 80cgg?gga?att?aag?cgt?gta?aca?gcg?ata?tta?tca?gtt?ttt?act?att?ata 288Arg?Gly?Ile?Lys?Arg?Val?Thr?Ala?Ile?Leu?Ser?Val?Phe?Thr?Ile?Ile

85 90 95atc?ctt?ctg?tta?att?tgg?gca?atg?tct?agt?ttt?att?ccc?atg?atg?agt 336Ile?Leu?Leu?Leu?Ile?Trp?Ala?Met?Ser?Ser?Phe?Ile?Pro?Met?Met?Ser

100 105 110aat?caa?tta?cgc?cat?ttt?atg?gaa?gat?ctc?cct?tca?tat?gtg?aat?aaa 384Asn?Gln?Leu?Arg?His?Phe?Met?Glu?Asp?Leu?Pro?Ser?Tyr?Val?Asn?Lys

115 120 125gtg?caa?atg?gaa?aca?agt?tcg?ttt?ata?gat?cac?aac?cct?tgg?tta?aaa 432Val?Gln?Met?Glu?Thr?Ser?Ser?Phe?Ile?Asp?His?Asn?Pro?Trp?Leu?Lys

130 135 140tct?tat?aaa?ggg?gaa?ata?tcg?agc?atg?tta?tct?aat?atc?agt?agc?caa 480Ser?Tyr?Lys?Gly?Glu?Ile?Ser?Ser?Met?Leu?Ser?Asn?Ile?Ser?Ser?Gln145 150 155 160gcg?gtc?tct?tat?gct?gaa?aaa?ttt?tca?aag?aat?gtt?tta?gat?tgg?gca 528Ala?Va1?Ser?Tyr?Ala?Glu?Lys?Phe?Ser?Lys?Asn?Val?Leu?Asp?Trp?Ala

165 170 175gga?aat?tta?gct?agt?aca?gtt?gca?cgt?gtg?aca?gta?gca?aca?atc?atg 576Gly?Asn?Leu?Ala?Ser?Thr?Val?Ala?Arg?Val?Thr?Val?Ala?Thr?Ile?Met

180 185 190gct?ccc?ttt?att?ttg?ttt?tat?ctt?tta?aga?gat?agt?cgc?aac?atg?aag 624Ala?Pro?Phe?Ile?Leu?Phe?Tyr?Leu?Leu?Arg?Asp?Ser?Arg?Asn?Met?Lys

195 200 205aat?ggt?ttc?tta?atg?gtt?tta?cca?acc?aaa?cta?cgc?caa?cca?gct?gat 672Asn?Gly?Phe?Leu?Met?Val?Leu?Pro?Thr?Lys?Leu?Arg?Gln?Pro?Ala?Asp

210 215 220cgt?att?ttg?cga?gaa?atg?aat?agt?caa?atg?tca?gga?tat?gtg?caa?gga 720Arg?Ile?Leu?Arg?Glu?Met?Asn?Ser?Gln?Met?Ser?Gly?Tyr?Val?Gln?Gly225 230 235 240caa?atc?att?gtt?gct?att?act?gtt?ggt?gtt?att?ttt?tca?ata?atg?tat 768Gln?Ile?Ile?Val?Ala?Ile?Thr?Val?Gly?Val?Ile?Phe?Ser?Ile?Met?Tyr

245 250 255agt?att?ata?ggc?ctt?aga?tat?ggc?gtg?aca?tta?ggg?att?att?gcc?ggt 816Ser?Ile?Ile?Gly?Leu?Arg?Tyr?Gly?Val?Thr?Leu?Gly?Ile?Ile?Ala?Gly

260 265 270gtg?tta?aat?atg?gtt?ccc?tat?ttg?gga?agt?ttt?gtc?gcc?caa?att?cca 864Val?Leu?Asn?Met?Val?Pro?Tyr?Leu?Gly?Ser?Phe?Val?Ala?Gln?Ile?Pro

275 280 285gtg?ttt?atc?tta?gcg?ctt?gtc?gca?gga?cct?gtt?atg?gtt?gtt?aaa?gtt 912Val?Phe?Ile?Leu?Ala?Leu?Val?Ala?Gly?Pro?Val?Met?Val?Val?Lys?Val

290 295 300gcg?att?gtt?ttt?gtt?att?gag?caa?act?cta?gag?gga?cgc?ttt?gtc?tca 960Ala?Ile?Val?Phe?Val?Ile?Glu?Gln?Thr?Leu?Glu?Gly?Arg?Phe?Val?Ser305 310 315 320cct?ttg?gtt?tta?ggt?aat?aaa?ctt?agc?att?cat?cca?att?aca?att?atg 1008Pro?Leu?Val?Leu?Gly?Asn?Lys?Leu?Ser?Ile?His?Pro?Ile?Thr?Ile?Met

325 330 335ttt?att?tta?tta?acc?tct?gga?gcg?atg?ttt?ggt?gtt?tgg?gga?gta?ttc 1056Phe?Ile?Leu?Leu?Thr?Ser?Gly?Ala?Met?Phe?Gly?Val?Trp?Gly?Val?Phe

340 345 350ctc?agt?att?ccg?att?tat?gca?tct?atc?aaa?gtt?gtt?gtt?aaa?gaa?ttg 1104Leu?Ser?Ile?Pro?Ile?Tyr?Ala?Ser?Ile?Lys?Val?Val?Val?Lys?Glu?Leu

355 360 365ttt?gat?tgg?tac?aaa?gct?gtc?agt?ggg?cta?tat?aca?ata?gat?gtt?gtt 1152Phe?Asp?Trp?Tyr?Lys?Ala?Val?Ser?Gly?Leu?Tyr?Thr?Ile?Asp?Val?Val

370 375 380act gaa gaa aga agt gaa gaa gtt aaa aat gtt gaa tag 1191Thr Glu Glu Arg Ser Glu Glu Val Lys Asn Val Glu385,390 395＜210〉29＜211〉396＜212〉PRT＜213〉B group streptococcus＜400〉29Val Asn Glu Ser Thr Ile Arg Lys Glu Phe Lys Ile Val Val Phe Lys 15 10 15Trp Ile Leu Asn Asn Gln Ala Val Ile Ala Leu Met Ile Thr Phe Leu

20 25 30Val?Phe?Leu?Thr?Ile?Phe?Ile?Phe?Thr?Lys?Ile?Ser?Phe?Met?Phe?Lys

35 40 45Pro?Val?Phe?Asp?Phe?Leu?Ala?Val?Leu?Ile?Leu?Pro?Leu?Val?Ile?Ser

50 55 60Gly?Leu?Leu?Tyr?Tyr?Leu?Leu?Lys?Pro?Met?Val?Thr?Phe?Leu?Glu?Lys?65 70 75 80Arg?Gly?Ile?Lys?Arg?Val?Thr?Ala?Ile?Leu?Ser?Val?Phe?Thr?Ile?Ile

85 90 95Ile?Leu?Leu?Leu?Ile?Trp?Ala?Met?Ser?Ser?Phe?Ile?Pro?Met?Met?Ser

100 105 110Asn?Gln?Leu?Arg?His?Phe?Met?Glu?Asp?Leu?Pro?Ser?Tyr?Val?Asn?Lys

115 120 125Val?Gln?Met?Glu?Thr?Ser?Ser?Phe?Ile?Asp?His?Asn?Pro?Trp?Leu?Lys

130 135 140Ser?Tyr?Lys?Gly?Glu?Ile?Ser?Ser?Met?Leu?Ser?Asn?Ile?Ser?Ser?Gln145 150 155 160Ala?Val?Ser?Tyr?Ala?Glu?Lys?Phe?Ser?Lys?Asn?Val?Leu?Asp?Trp?Ala

165 170 175Gly?Asn?Leu?Ala?Set?Thr?Val?Ala?Arg?Val?Thr?Val?Ala?Thr?Ile?Met

180 185 190Ala?Pro?Phe?Ile?Leu?Phe?Tyr?Leu?Leu?Arg?Asp?Ser?Arg?Asn?Met?Lys

195 200 205Asn?Gly?Phe?Leu?Met?Val?Leu?Pro?Thr?Lys?Leu?Arg?Gln?Pro?Ala?Asp

210 215 220Arg?Ile?Leu?Arg?Glu?Met?Asn?Ser?Gln?Met?Ser?Gly?Tyr?Val?Gln?Gly225 230 235 240Gln?Ile?Ile?Val?Ala?Ile?Thr?Val?Gly?Val?Ile?Phe?Ser?Ile?Met?Tyr

245 250 255Ser?Ile?Ile?Gly?Leu?Arg?Tyr?Gly?Val?Thr?Leu?Gly?Ile?Ile?Ala?Gly

260 265 270Val?Leu?Asn?Met?Val?Pro?Tyr?Leu?Gly?Ser?Phe?Val?Ala?Gln?Ile?Pro

275 280 285Val?Phe?Ile?Leu?Ala?Leu?Val?Ala?Gly?Pro?Val?Met?Val?Val?Lys?Val

290 295 300Ala?Ile?Val?Phe?Val?Ile?Glu?Gln?Thr?Leu?Glu?Gly?Arg?Phe?Val?Ser305 310 315 320Pro?Leu?Val?Leu?Gly?Asn?Lys?Leu?Ser?Ile?His?Pro?Ile?Thr?Ile?Met

325 330 335Phe?Ile?Leu?Leu?Thr?Ser?Gly?Ala?Met?Phe?Gly?Val?Trp?Gly?Val?Phe

340 345 350Leu?Ser?Ile?Pro?Ile?Tyr?Ala?Ser?Ile?Lys?Val?Val?Val?Lys?Glu?Leu

355 360 365Phe?Asp?Trp?Tyr?Lys?Ala?Val?Ser?Gly?Leu?Tyr?Thr?Ile?Asp?Val?Val

370 375 380Thr Glu Glu Arg Ser Glu Glu Val Lys Asn Val Glu385,390 395＜210〉30＜211〉1230＜212〉DNA＜213〉B group streptococcus＜220〉＜221〉CDS＜222〉(1) .. (1230)＜400〉30atg ttt atg gga atc cca caa tat ttc ttc tac ctt atc tta gct gtc 48Met Phe Met Gly Ile Pro Gln Tyr Phe Phe Tyr Leu Ile Leu Ala Val 15 10 15eta cca att tac atc ggc tta ttc ttt aag aag cgt ttt gcc tta tat 96Leu Pro Ile Tyr Ile Gly Leu Phe Phe Lys Lys Arg Phe Ala Leu Tyr

20 25 30gag?att?att?ttt?agt?cta?agt?ttt?att?gta?atg?atg?ttg?act?ggt?agt 144Glu?Ile?Ile?Phe?Ser?Leu?Ser?Phe?Ile?Val?Met?Met?Leu?Thr?Gly?Ser

35 40 45act?ttt?aat?caa?ttg?aag?tca?cta?ttg?gca?tac?gtt?gtc?gga?cag?tct 192Thr?Phe?Asn?Gln?Leu?Lys?Ser?Leu?Leu?Ala?Tyr?Val?Val?Gly?Gln?Ser

50 55 60ctg?cta?gtt?ttt?atc?tat?aaa?gct?tac?cgg?aaa?cga?ttt?aat?cat?act 240Leu?Leu?Val?Phe?Ile?Tyr?Lys?Ala?Tyr?Arg?Lys?Arg?Phe?Asn?His?Thr?65 70 75 80ttg?gtc?ttt?tat?gta?acg?gtt?tgt?tta?tct?att?ttt?ccg?cta?ttt?ttg 288Leu?Val?Phe?Tyr?Val?Thr?Val?Cys?Leu?Ser?Ile?Phe?Pro?Leu?Phe?Leu

85 90 95gta?aaa?tta?att?cca?gct?ara?tct?gag?gat?ggg?cat?cag?tca?ctt?ttt 336Val?Lys?Leu?Ile?Pro?Ala?Ile?Ser?Glu?Asp?Gly?His?Gln?Ser?Leu?Phe

100 105 110ggg?ttt?cta?gga?att?tct?tac?ctt?act?ttt?aga?gct?gtt?gct?atg?att 384Gly?Phe?Leu?Gly?Ile?Ser?Tyr?Leu?Thr?Phe?Arg?Ala?Val?Ala?Met?Ile

115 120 125att?gaa?atg?aga?gac?ggt?gtc?ttg?aaa?gaa?ttt?act?tta?tgg?gaa?ttc 432Ile?Glu?Met?Arg?Asp?Gly?Val?Leu?Lys?Glu?Phe?Thr?Leu?Trp?Glu?Phe

130 135 140tta?aga?ttt?tta?ctc?ttc?ttt?cca?act?ttc?tca?agt?gga?cca?att?gat 480Leu?Arg?Phe?Leu?Leu?Phe?Phe?Pro?Thr?Phe?Ser?Ser?Gly?Pro?Ile?Asp145 150 155 160cgt?ttt?aaa?cga?ttc?aat?gag?gat?tac?att?aat?atc?cca?gat?cga?aac 528Arg?Phe?Lys?Arg?Phe?Asn?Glu?Asp?Tyr?Ile?Asn?Ile?Pro?Asp?Arg?Asn

165 170 175gaa?ctc?cta?gat?atg?tta?ggt?caa?gcg?att?cat?tat?ttg?atg?tta?ggt 576Glu?Leu?Leu?Asp?Met?Leu?Gly?Gln?Ala?Ile?His?Tyr?Leu?Met?Leu?Gly

180 185 190ttt?ctc?tat?aag?ttt?att?tta?gcc?tat?att?ttt?gga?agt?ctg?att?atg 624Phe?Leu?Tyr?Lys?Phe?Ile?Leu?Ala?Tyr?Ile?Phe?Gly?Ser?Leu?Ile?Met

195 200 205cct?cct?cta?aaa?gaa?tta?gcg?cta?gaa?cag?ggt?ggt?gtg?ttt?aat?tgg 672Pro?Pro?Leu?Lys?Glu?Leu?Ala?Leu?Glu?Gln?Gly?Gly?Val?Phe?Asn?Trp

210 215 220cca?aca?crt?ggg?gtt?atg?tat?gcc?ttt?ggt?ttt?gat?ttg?ttc?ttt?gat 720Pro?Thr?Leu?Gly?Val?Met?Tyr?Ala?Phe?Gly?Phe?Asp?Leu?Phe?Phe?Asp225 230 235 240ttt?gca?ggt?tac?aca?atg?ttt?gcg?ttg?gct?att?tct?aac?cra?atg?ggg 768Phe?Ala?Gly?Tyr?Thr?Met?Phe?Ala?Leu?Ala?Ile?Ser?Asn?Leu?Met?Gly

245 250 255att?aag?tct?ccg?att?aac?ttt?gac?aaa?cct?ttc?aaa?tca?cgc?gac?cta 816Ile?Lys?Ser?Pro?Ile?Asn?Phe?Asp?Lys?Pro?Phe?Lys?Ser?Arg?Asp?Leu

260 265 270aaa?gaa?ttt?tgg?aat?aga?tgg?cat?atg?agc?ctt?tct?ttc?tgg?ttt?aga 864Lys?Glu?Phe?Trp?Asn?Arg?Trp?His?Met?Ser?Leu?Ser?Phe?Trp?Phe?Arg

275 280 285gac?ttt?gtt?ttc?atg?agg?ctt?gtt?aag?ctt?tta?gtt?aaa?aat?aaa?gtt 912Asp?Phe?Val?Phe?Met?Arg?Leu?Val?Lys?Leu?Leu?Val?Lys?Asn?Lys?Val

290 295 300ttt?aaa?aac?cgt?aat?gtt?act?tca?agt?gta?gct?tat?att?atc?aat?atg 960Phe?Lys?Asn?Arg?Asn?Val?Thr?Ser?Ser?Val?Ala?Tyr?Ile?Ile?Asn?Met305 310 315 320ctt?ctt?atg?gga?ttc?tgg?cat?ggg?tta?act?tgg?tac?tat?ata?gcc?tat 1008Leu?Leu?Met?Gly?Phe?Trp?His?Gly?Leu?Thr?Trp?Tyr?Tyr?Ile?Ala?Tyr

325 330 335ggt?ctc?ttt?cat?ggg?att?ggc?cta?gtt?att?aat?gac?gct?tgg?gta?cgt 1056Gly?Leu?Phe?His?Gly?Ile?Gly?Leu?Val?Ile?Asn?Asp?Ala?Trp?Val?Arg

340 345 350aag?aag?aaa?aat?ayt?aat?aaa?gaa?aga?aga?ttg?gct?aaa?aaa?cca?ctt 1104Lys?Lys?Lys?Asn?Xaa?Asn?Lys?Glu?Arg?Arg?Leu?Ala?Lys?Lys?Pro?Leu

355 360 365tta?cca?gaa?aac?aaa?tgg?act?tat?gct?ttg?ggt?gtc?ttc?atc?acc?ttt 1152Leu?Pro?Glu?Asn?Lys?Trp?Thr?Tyr?Ala?Leu?Gly?Val?Phe?Ile?Thr?Phe

370 375 380aat?gta?gtt?atg?ttt?tct?ttc?ttg?att?ttt?tca?gga?ttt?tta?gat?ctt 1200Asn?Val?Val?Met?Phe?Ser?Phe?Leu?Ile?Phe?Ser?Gly?Phe?Leu?Asp?Leu385 390 395 400ttg?tgg?ttc?cca?caa?ccg?cat?aac?aaa?taa 1230Leu?Trp?Phe?Pro?Gln?Pro?His?Asn?Lys

405 410＜210〉31＜211〉409＜212〉PRT＜213〉B group streptococcus＜400〉31Met Phe Met Gly Ile Pro Gln Tyr Phe Phe Tyr Leu Ile Leu Ala Val 15 10 15Leu Pro Ile Tyr Ile Gly Leu Phe Phe Lys Lys Arg Phe Ala Leu Tyr

20 25 30Glu?Ile?Ile?Phe?Ser?Leu?Ser?Phe?Ile?Val?Met?Met?Leu?Thr?Gly?Ser

35 40 45Thr?Phe?Asn?Gln?Leu?Lys?Ser?Leu?Leu?Ala?Tyr?Val?Val?Gly?Gln?Ser

50 55 60Leu?Leu?Val?Phe?Ile?Tyr?Lys?Ala?Tyr?Arg?Lys?Arg?Phe?Asn?His?Thr?65 70 75 80Leu?Val?Phe?Tyr?Val?Thr?Val?cys?Leu?Ser?Ile?Phe?Pro?Leu?Phe?Leu

85 90 95Val?Lys?Leu?Ile?Pro?Ala?Ile?Ser?Glu?Asp?Gly?His?Gln?Ser?Leu?Phe

100 105 110Gly?Phe?Leu?Gly?Ile?Ser?Tyr?Leu?Thr?Phe?Arg?Ala?Val?Ala?Met?Ile

115 120 125Ile?Glu?Met?Arg?Asp?Gly?Val?Leu?Lys?Glu?Phe?Thr?Leu?Trp?Glu?Phe

130 135 140Leu?Arg?Phe?Leu?Leu?Phe?Phe?Pro?Thr?Phe?Set?Ser?Gly?Pro?Ile?Asp145 150 155 160Arg?Phe?Lys?Arg?Phe?Asn?Glu?Asp?Tyr?Ile?Asn?Ile?Pro?Asp?Arg?Asn

165 170 175Glu?Leu?Leu?Asp?Met?Leu?Gly?Gln?Ala?Ile?His?Tyr?Leu?Met?Leu?Gly

180 185 190Phe?Leu?Tyr?Lys?Phe?Ile?Leu?Ala?Tyr?Ile?Phe?Gly?Ser?Leu?Ile?Met

195 200 205Pro?Pro?Leu?Lys?Glu?Leu?Ala?Leu?Glu?Gln?Gly?Gly?Val?Phe?Asn?Trp

210 215 220Pro?Thr?Leu?Gly?Val?Met?Tyr?Ala?Phe?Gly?Phe?Asp?Leu?Phe?Phe?Asp225 230 235 240Phe?Ala?Gly?Tyr?Thr?Met?Phe?Ala?Leu?Ala?Ile?Ser?Asn?Leu?Met?Gly

245 250 255Ile?Lys?Ser?Pro?Ile?Asn?Phe?Asp?Lys?Pro?Phe?Lys?Ser?Arg?Asp?Leu

260 265 270Lys?Glu?Phe?Trp?Asn?Arg?Trp?His?Met?Ser?Leu?Ser?Phe?Trp?Phe?Arg

275 280 285Asp?Phe?Val?Phe?Met?Arg?Leu?Val?Lys?Leu?Leu?Val?Lys?Asn?Lys?Val

290 295 300Phe?Lys?Asn?Arg?Asn?Val?Thr?Ser?Ser?Val?Ala?Tyr?Ile?Ile?Asn?Met305 310 315 320Leu?Leu?Met?Gly?Phe?Trp?His?Gly?Leu?Thr?Trp?Tyr?Tyr?Ile?Ala?Tyr

325 330 335Gly?Leu?Phe?His?Gly?Ile?Gly?Leu?Val?Ile?Asn?Asp?Ala?Trp?Val?Arg

340 345 350Lys?Lys?Lys?Asn?Xaa?Asn?Lys?Glu?Arg?Arg?Leu?Ala?Lys?Lys?Pro?Leu

355 360 365Leu?Pro?Glu?Asn?Lys?Trp?Thr?Tyr?Ala?Leu?Gly?Val?Phe?Ile?Thr?Phe

370 375 380Asn?Val?Val?Met?Phe?Ser?Phe?Leu?Ile?Phe?Ser?Gly?Phe?Leu?Asp?Leu385 390 395 400Leu?Trp?Phe?Pro?Gln?Pro?His?Asn?Lys

405＜210〉32＜211〉100＜212〉DNA＜213〉B group streptococcus＜220〉＜221〉CDS＜222〉(1) .. (99)＜400〉32atg aat aaa ata acg aca tta tca acc atc gcc ctg act tta atg ctt 48Met Asn Lys Ile Thr Thr Leu Ser Thr Ile Ala Leu Thr Leu Met Leu 15 10 15tgc gtt gga tgt tct gcc aat aaa gat aat caa aaa act aaa act gag 96Cys Val Gly Cys Ser Ala Asn Lys Asp Asn Gln Lys Thr Lys Thr Glu

20 25 30gat c 100Asp＜210〉33＜211〉33＜212〉PRT＜213〉B group streptococcus＜400〉33Met Asn Lys Ile Thr Thr Leu Ser Thr Ile Ala Leu Thr Leu Met Leu 15 10 15Cys Val Gly Cys Ser Ala Asn Lys Asp Asn Gln Lys Thr Lys Thr Glu

20 25 30Asp＜210〉34＜211〉654＜212〉DNA＜213〉B group streptococcus＜220〉＜221〉CDS＜222〉(1) .. (654)＜400〉34gat cga ggc tat caa gaa gca atg gct aaa cta agg aaa act tac ggc 48Asp Arg Gly Tyr Gln Glu Ala Met Ala Lys Leu Arg Lys Thr Tyr Gly 15 10 15gaa tat ggt tta ggg gtt tct aca gga tta gat tta cct gaa tca gaa 96Glu Tyr Gly Leu Gly Val Ser Thr Gly Leu Asp Leu Pro Glu Ser Glu

20 25 30ggt?tat?gta?cct?gga?aaa?tac?agc?tta?gga?aca?act?cta?atg?gaa?tcg 144Gly?Tyr?Val?Pro?Gly?Lys?Tyr?Ser?Leu?Gly?Thr?Thr?Leu?Met?Glu?Ser

35 40 45ttc?ggt?cag?tat?gat?gcc?tat?aca?cca?atg?caa?ctt?ggt?cag?tat?atc 192Phe?Gly?Gln?Tyr?Asp?Ala?Tyr?Thr?Pro?Met?Gln?Leu?Gly?Gln?Tyr?Ile

50 55 60tca?act?att?gcg?aat?aat?ggg?aat?cgt?tta?gca?cct?cac?gtg?gtt?tca 240Ser?Thr?Ile?Ala?Asn?Asn?Gly?Asn?Arg?Leu?Ala?Pro?His?Val?Val?Ser?65 70 75 80gat?atc?tat?gaa?ggg?aat?gat?tct?aat?aag?ttc?gct?caa?ttg?gtt?cgt 288Asp?Ile?Tyr?Glu?Gly?Asn?Asp?Ser?Asn?Lys?Phe?Ala?Gln?Leu?Val?Arg

85 90 95tca?atc?act?cct?aaa?aca?cta?aat?aag?ata?gct?atc?tca?gat?caa?gag 336Ser?Ile?Thr?Pro?Lys?Thr?Leu?Asn?Lys?IleA?la?Ile?Ser?Asp?Gln?Glu

100 105 110tta?gcc?att?att?caa?gaa?ggt?ttt?tat?aac?gtt?gtc?aat?agt?gga?agt 384Leu?Ala?Ile?Ile?Gln?Glu?Gly?Phe?Tyr?Asn?Val?Val?Asn?Ser?Gly?Ser

115 120 125ggc?tat?gca?act?ggt?acg?tca?atg?agg?ggg?aat?gtg?aca?acc?att?agy 432Gly?Tyr?Ala?Thr?Gly?Thr?Ser?Met?Arg?Gly?Asn?Val?Thr?Thr?Ile?Xaa

130 135 140ggt?aaa?act?ggt?acc?gct?gaa?aca?ttt?gct?aaa?aat?ata?aat?gga?caa 480Gly?Lys?Thr?Gly?Thr?Ala?Glu?Thr?Phe?Ala?Lys?Asn?Ile?Asn?Gly?Gln145 150 155 160aca?gtt?tct?acc?tac?aac?tta?aac?gct?att?gcc?tac?gat?act?aat?cgt 528Thr?Val?Ser?Thr?Tyr?Asn?Leu?Asn?Ala?Ile?Ala?Tyr?Asp?Thr?Asn?Arg

165 170 175aaa?ata?gca?gta?gcg?gta?atg?tat?ccg?cat?gtt?aca?act?gat?aca?aca 576Lys?Ile?Ala?Val?Ala?Val?Met?Tyr?Pro?His?Val?Thr?Thr?Asp?Thr?Thr

180 185 190aaa?tcc?cat?caa?tta?gtt?gca?cga?gat?atg?att?gat?caa?tat?att?tca 624Lys?Ser?His?Gln?Leu?Val?Ala?Arg?Asp?Met?Ile?Asp?Gln?Tyr?Ile?Ser

195 200 205cag?tca?cag?gac?aat?aag?aga?gga?cat?tga 654Gln?Ser?Gln?Asp?Asn?Lys?Arg?Gly?His

210 215＜210〉35＜211〉217＜212〉PRT＜213〉B group streptococcus＜400〉35Asp Arg Gly Tyr Gln Glu Ala Met Ala Lys Leu Arg Lys Thr Tyr Gly l 5 10 15Glu Tyr Gly Leu Gly Val Ser Thr Gly Leu Asp Leu Pro Glu Ser Glu

20 25 30Gly?Tyr?Val?Pro?Gly?Lys?Tyr?Ser?Leu?Gly?Thr?Thr?Leu?Met?Glu?Ser

35 40 45Phe?Gly?Gln?Tyr?Asp?Ala?Tyr?Thr?Pro?Met?Gln?Leu?Gly?Gln?Tyr?Ile

50 55 60Ser?Thr?Ile?Ala?Asn?Asn?Gly?Asn?Arg?Leu?Ala?Pro?His?Val?Val?Ser?65 70 75 80Asp?Ile?Tyr?Glu?Gly?Asn?Asp?Ser?Asn?Lys?Phe?Ala?Gln?Leu?Val?Arg

85 90 95Ser?Ile?Thr?Pro?Lys?Thr?Leu?Asn?Lys?Ile?Ala?Ile?Ser?Asp?Gln?Glu

100 105 110Leu?Ala?Ile?Ile?Gln?Glu?Gly?Phe?Tyr?Asn?Val?Val?Asn?Ser?Gly?Ser

115 120 125Gly?Tyr?Ala?Thr?Gly?Thr?Ser?Met?Arg?Gly?Asn?Val?Thr?Thr?Ile?Xaa

130 135 140Gly?Lys?Thr?Gly?Thr?Ala?Glu?Thr?Phe?Ala?Lys?Asn?Ile?Asn?Gly?Gln145 150 155 160Thr?Val?Ser?Thr?Tyr?Asn?Leu?Asn?Ala?Ile?Ala?Tyr?Asp?Thr?Asn?Arg

165 170 175Lys?Ile?Ala?Val?Ala?Val?Met?Tyr?Pro?His?Val?Thr?Thr?Asp?Thr?Thr

180 185 190Lys?Ser?His?Gln?Leu?Val?Ala?Arg?Asp?Met?Ile?Asp?Gln?Tyr?Ile?Ser

195 200 205Gln?Ser?Gln?Asp?Asn?Lys?Arg?Gly?His

210 215

Claims (12)

1. one kind by comprising of can obtaining be accredited as phol-13 herein, pho3-21, pho2-15 from the B group streptococcus, pho3-18, pho3-22, pho3-3, pho3-17, pho2-2, pho1-5, pho3-1, pho3-23, pho3-50, pho1-14, pho2-10, pho3-14, the peptide of the operon of any or its homologue or its functional fragment coding in the gene of pho3-24 and ho3-29.

2. the peptide of claim 1 is included in and is accredited as SEQ ID NOS.2 herein, arbitrary aminoacid sequence of 4,6,8,10,11,13,15,17,19,21,23,25,27,29,31,33 and 35.

3. be used for the treatment of the claim 1 of purposes or the peptide of claim 2.

4. a coding is used for the treatment of the polynucleotide of the peptide of the claim 1 of purposes or claim 2.

5. conversion host who explains the peptide of claim 1 or claim 2.

6. a vaccine comprises the peptide of claim 1 or claim 2, or is used for the instrument of its expression.

7. the product of any one is used to screen potential drug or be used for the usefulness that toxicity detects among the claim 1-5.

Among the claim 1-5 product of any one preparation be used for the treatment of or the medicine of the illness that prevention is relevant with infectation of bacteria in purposes.

9. the purposes of claim 8, infection wherein are that the B group streptococcus infects.

10. the purposes of claim 8 or claim 9, infection wherein is focal infection.

11. the purposes of claim 8 or claim 9, infection wherein is urinary tract infection.

12. the antibody that peptide produced at claim 1 or claim 2.