CN1324955A - Method of using ribonucleic acid as product antifake mark and for verification - Google Patents
Method of using ribonucleic acid as product antifake mark and for verification Download PDFInfo
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- CN1324955A CN1324955A CN 00107580 CN00107580A CN1324955A CN 1324955 A CN1324955 A CN 1324955A CN 00107580 CN00107580 CN 00107580 CN 00107580 A CN00107580 A CN 00107580A CN 1324955 A CN1324955 A CN 1324955A
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- nucleic acid
- yeast nucleic
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Abstract
The present invention discloses a method using ribonucleic acid as product anti-false mark and its proof technique. and is characterized by that when it is made, the medium containing ribonucleic acid can be marked on the target product, and when the mark is to be proved, the medium is taken off, the ribonucleic acid is extracted and recovered by means of solvent, then the ribonucleic acid signal can be amplified by PCR technique and proved, it can identify target product according to the proof result so as to attain the goal of distinguishing the true from the false.
Description
The present invention be for a kind of tagged items to reach the method for false proof purpose, refer in particular to a kind of method of utilizing Yeast Nucleic Acid as product anti-fake mark and checking, the medium that will be mixed with Yeast Nucleic Acid during making is marked on the target compound, during check medium is taken off, use solvent that the Yeast Nucleic Acid extraction is reclaimed, again with polymerase chain reaction with Yeast Nucleic Acid signal amplified inspection, the true or false of the target compound that is labeled with differentiation according to the result.
Is exactly the problem that product is counterfeit and forge in the product manufacturing with being on sale throughout one of recurrent problem.Counterfeit and counterfeit is made use of outward appearance, the brand of genuine piece, and the image of utilizing genuine piece is to reach the purpose of making profit; Because just outward appearance and brand are similar usually for counterfeit merchandise and counterfeit, but made with the low material of economizing; The counterfeit of selling approximate genuine piece quality is also arranged, but because of must not spending the cost of advertisement and marketing, thereby the market of can lower price capturing genuine piece.In addition, all have high monovalent article, as famous painting bullion and souvenir etc., and the article with financial function and value, also often face the counterfeit problem of forging as credit card, bankbook and Valued Securities etc.Problem like that not only Valued Securities etc. often faces and forges counterfeit article, also often faces the counterfeit problem of forging as credit card, bankbook and Valued Securities etc.Problem like that not only can be destroyed the goodwill of genuine piece and influence the sale of genuine piece, and What is more can hinder the financial order of a country and the ability of innovation, so how to hit counterfeit and counterfeit, prevents that genuine piece from being copied, and its demand and necessity are arranged in fact.The existing method that prevents to forge is except the cancellation fee person that fights with obviously special moulding own and quality trusts, also have in the mode that adds and reach false proof purpose, such as the magnetic bar code on the bankbook, the laser label pattern on the fiscard and for example, with the secret mark that under special wavelength, just shows etc., also have add with protein antibody fluorescent substance etc. all on shielded object, with the particular form check to reach the purpose of identifying; Means purpose like that all makes at the obstacle of setting up a technology or method makes counterfeit merchandise and the difficult imitation easily of counterfeit person; known method provides the protection of technology barrier, but this type of protection still can be had the imitation easily of constructed ability person institute.
So purpose of the present invention provides a kind of specificity that has more, and can't be had constructed ability person counterfeit method for anti-counterfeit.
For this reason, the present invention utilizes Yeast Nucleic Acid (ribonucleic acid RNA) to have the narrow spectrum characteristic of sequence, with Yeast Nucleic Acid and Jie hold mixed after, have the medium of Yeast Nucleic Acid can be applied on easily by false proof target compound or ooze in target compound, the Yeast Nucleic Acid that has on the test-target thing again can be identified target compound and whether is institute's mark originally.
The characteristic that medium has for can with the Yeast Nucleic Acid thorough mixing, and the some that does not belong to target compound, the composition of Yeast Nucleic Acid is through design, can have particular length and sequence, and this sequence only uses when detecting specific polymerase chain reaction introduction just can detect the Yeast Nucleic Acid true and false that the checking medium has.
Concrete technical scheme of the present invention is: a kind of Yeast Nucleic Acid is as the method for product anti-fake mark and checking, it is characterized in that Yeast Nucleic Acid is stored in the medium, the medium that will contain Yeast Nucleic Acid directly is marked on the target compound or in the target compound, or the medium that will contain Yeast Nucleic Acid is used further to mark after being added in the liquid or solid; The mode of utilizing solvent recuperation and polymerase chain reaction to amplify Yeast Nucleic Acid during check reaches the true and false that authenticates the target compound that is labeled.
Feature of the present invention is that also described Yeast Nucleic Acid is DNA (desoxyribose nucleic acid) or Yeast Nucleic Acid.Alleged Yeast Nucleic Acid is from animal, plant, bacterium, virus or by synthetic carrier or fragment.Described medium is meant inertia and interactive material is not taken place target compound.Described medium be meant can with Yeast Nucleic Acid blended polymkeric substance.Alleged polymkeric substance is acryl (acrylic acrylplastics) or plastic cement.Described liquid or solid is meant ink, glue or polymkeric substance.Alleged ink is oiliness or water-based.Alleged tackiness agent is oiliness or water-based.Alleged polymkeric substance is acryl or plastic cement.The method of described recovery is with organic solvent or inorganic solvent the Yeast Nucleic Acid extraction to be reclaimed.Described organic solvent is buffer reagent, toluene, kerosene, alcohol, acetone or chloroform.Described inorganic solvent is a water.Described buffer reagent is TE (Tris-EDTA) buffer reagent.The method that described polymerase chain reaction amplifies Yeast Nucleic Acid is to be single or multiple (nested) polymerase chain reaction.
Earlier medium is dissolved in the solvent during operation, makes medium liquefaction, the Yeast Nucleic Acid with quantitative and known array adds uniform mixing in the medium solution again, and the medium solution that has Yeast Nucleic Acid this moment can be used for coating or filling target compound; Treat after the solvent evaporation that target compound promptly has mixed the medium of Yeast Nucleic Acid, in case whether will verify object is when having the target compound of specific RNA sequence, medium on the target compound can be got sub-fraction is dissolved in the solvent, the solvent that adds the high Yeast Nucleic Acid solubleness of another tool again but do not influence target compound makes it thorough mixing, this moment, most Yeast Nucleic Acid can be dissolved in the solvent of high Yeast Nucleic Acid solubleness, re-use centrifugation method the different solvents layering is separated taking-up, this moment, the Yeast Nucleic Acid concentration through extracting was enough verified the true and false of Yeast Nucleic Acid with the specific introduction of polymerase chain reaction; By this, if inspected target compound has the original Yeast Nucleic Acid of putting into, then polymerase chain reaction can amplify the original Yeast Nucleic Acid of putting into and become the millions of times of Yeast Nucleic Acid that size is identical, otherwise examined object is not if have primary Yeast Nucleic Acid, then polymerase chain reaction can't carry out the amplification reaction of Yeast Nucleic Acid, so just can distinguish that by the comparison of product size and quantity whether the target compound of wanting identification was by being indicated originally.
Because RNA sequence has specificity, when carrying out the polymerase chain reaction checking, only react and just can obtain correct nucleic acid size with single-minded introduction, extremely low because of the Yeast Nucleic Acid concentration of sneaking into medium again, can't change the mode of growing with gene and learn sequence and the size of putting into nucleic acid, so confidentiality and specificity are all high.
Further elaborate the present invention below.
The present invention is the sequence characteristic that utilizes Yeast Nucleic Acid to have, unless know sequence two ends content otherwise the principle that can't duplicate, it is that Yeast Nucleic Acid is stored in the medium, medium is marked on is used as the identity mark on the target compound again.When wanting to verify the true and false of target compound sometime, only medium need be taken off and check the Yeast Nucleic Acid that includes to differentiate the true and false of target compound.
So-called Yeast Nucleic Acid is the common name of DNA (desoxyribose nucleic acid) and Yeast Nucleic Acid, the source of Yeast Nucleic Acid can be from organic-biologicals such as animal, plant, bacterium, viruses, but also can synthetic carrier or segmental Yeast Nucleic Acid, the characteristic of Yeast Nucleic Acid is that the special sequence that it has can use corresponding introduction, duplicated with polymerase chain reaction, could design suitable introduction and carry out replication reaction but prerequisite is the sequence that must know the replicating nucleic acid fragment two ends of wanting.
So-called medium is exactly the intermediary's material that is used for comprising Yeast Nucleic Acid and is used for adhering to or is mixed in other objects, good medium must mix uniformly with Yeast Nucleic Acid, and the Yeast Nucleic Acid of wanting to screen is not damaged, and medium also wants energy tool plasticity-and suitable intensity also can be easy to be attached on the object that is labeled.
So-called target compound is exactly to be labeled false proof article, can be liquid or solid; Liquid person such as oil products such as lubricating oil, gasoline; Solid person such as antique, famous painting, bullion, financial credit card etc. have the article of commemorating or actual value being arranged, and can be target compounds.
The method of mark can be medium to be coated the surface of target compound, and for example credit card can be to be mixed in the object, and for example the ink oil painting can be to be filled in the object, and for example seal etc. can be designed because of actual needs.
Below be illustrated with regard to embodiments of the invention.
Fig. 1 shows with the polycarbonate medium, and the 800bp DNA (desoxyribose nucleic acid) is marked on the plastic film, signal is amplified the embodiment that obtains the 800bp DNA (desoxyribose nucleic acid) again through the electrophoretic separation poststaining with polymerase chain reaction after reclaiming.
It is medium that Fig. 2 shows with the polycarbonate, and human DNA (desoxyribose nucleic acid) genome is marked on the plastic film, signal is amplified the embodiment that obtains the 600bp DNA (desoxyribose nucleic acid) again through the electrophoretic separation poststaining with polymerase chain reaction after reclaiming.
Embodiment 1: be that medium is marked on the 800bp DNA (desoxyribose nucleic acid) on the plastic film with the polycarbonate.
Material: the polycarbonate Taiwan polycarbonate of Du Pont
95% alcohol, Pharmacopoea Chinensis
Acetone Taiwan Merck UN1090
Chloroform Taiwan Merck UN1888
With the DNA (desoxyribose nucleic acid) of the synthetic 800bp of polymerase chain reaction institute with alcohol add acetone solution it, be applied on the plastic film again with behind the polycarbonate thorough mixing that is dissolved in chloroform.Be positioned over 40 ℃ of refrigerators, shady places after the drying or tan by the sun in sunlight next day after reclaim.Cut a spot of diaphragm during recovery, dissolve it with chloroform, again with the damping fluid thorough mixing and centrifugal after obtain clear liquor.The clear liquor that takes a morsel carries out polymerase chain reaction as template.The product of polymerase chain reaction again through the electrophoretic separation poststaining it.See also Fig. 1, show with the polycarbonate to be medium, the 800bp DNA (desoxyribose nucleic acid) is the embodiment of mark.L1 is a 100bp DNA (desoxyribose nucleic acid) standard from left to right, and L2 is dark the processing, and L3, L4 and L5 handle down for tanning by the sun in sunlight, and L6, L7 and L8 are that 40 ℃ of refrigerators are handled.The result shows and all handles all as expected the result reclaims the 800bp DNA (desoxyribose nucleic acid) smoothly.
Embodiment 2: be that medium is marked on human DNA (desoxyribose nucleic acid) genome on the plastic film with the polycarbonate.
Material: the polycarbonate Taiwan polycarbonate of Du Pont
95% alcohol, Pharmacopoea Chinensis
Acetone Taiwan Merck UN1090
Chloroform Taiwan Merck UN1888
The DNA (desoxyribose nucleic acid) genome that will extract from human leukocyte with alcohol add acetone solution it, be applied on the plastic film again with behind the polycarbonate thorough mixing that is dissolved in chloroform.Be positioned over 40 ℃ of refrigerators, shady places after the drying or tan by the sun in sunlight next day after reclaim.Cut a spot of diaphragm during recovery, dissolve it with chloroform, again with the damping fluid thorough mixing and centrifugal after obtain clear liquor.The clear liquor that takes a morsel carries out polymerase chain reaction as template.The generation thing of polymerase chain reaction again through the electrophoretic separation poststaining it.See also Fig. 2, show with the polycarbonate to be medium, the human genome DNA (desoxyribose nucleic acid) is the embodiment of mark.L1 is a 100bp DNA (desoxyribose nucleic acid) standard from left to right, L2 and L3 are the result of L μ l clear liquor as template, L4 and L5 are that 2 μ l are the result of clear liquor as template, and L6 is not for adding the negative control group of template, and L7 is the positive regulation group of human genome DNA (desoxyribose nucleic acid).The result shows that except L3 is more not obvious all are handled and reclaim the 600bp Human genome all as expected smoothly.
Claims (15)
1, a kind of Yeast Nucleic Acid is as the method for product anti-fake mark and checking, it is characterized in that Yeast Nucleic Acid is stored in the medium, the medium that will contain Yeast Nucleic Acid directly is marked on the target compound or in the target compound, or the medium that will contain Yeast Nucleic Acid is used further to mark after being added in the liquid or solid; The mode of utilizing solvent recuperation and polymerase chain reaction to amplify Yeast Nucleic Acid during check reaches the true and false that authenticates the target compound that is labeled.
2, Yeast Nucleic Acid as claimed in claim 1 is characterized in that as the method for product anti-fake mark and checking described Yeast Nucleic Acid is DNA (desoxyribose nucleic acid) or Yeast Nucleic Acid.
3, Yeast Nucleic Acid as claimed in claim 1 is characterized in that alleged Yeast Nucleic Acid from animal, plant, bacterium as the method for product anti-fake mark and checking, virus or by synthetic carrier or fragment.
4, Yeast Nucleic Acid as claimed in claim 1 is characterized in that as the method for product anti-fake mark and checking described medium is meant inertia and interactive material is not taken place target compound.
5, Yeast Nucleic Acid as claimed in claim 1 is as the method for product anti-fake mark and checking, it is characterized in that described medium be meant can with Yeast Nucleic Acid blended polymkeric substance.
6, Yeast Nucleic Acid as claimed in claim 5 is characterized in that as the method for product anti-fake mark and checking alleged polymkeric substance is acryl or plastic cement.
7, Yeast Nucleic Acid as claimed in claim 1 is characterized in that as the method for product anti-fake mark and checking described liquid or solid is meant ink, glue or polymkeric substance.
8, Yeast Nucleic Acid as claimed in claim 7 is characterized in that as the method for product anti-fake mark and checking alleged ink is oiliness or water-based.
9, Yeast Nucleic Acid as claimed in claim 7 is characterized in that as the method for product anti-fake mark and checking alleged tackiness agent is oiliness or water-based.
10, Yeast Nucleic Acid as claimed in claim 7 is characterized in that as the method for product anti-fake mark and checking alleged polymkeric substance is acryl or plastic cement.
11, Yeast Nucleic Acid as claimed in claim 1 is as the method for product anti-fake mark and checking, and the method that it is characterized in that described recovery is with organic solvent or inorganic solvent the Yeast Nucleic Acid extraction to be reclaimed.
12, Yeast Nucleic Acid as claimed in claim 11 is characterized in that as the method for product anti-fake mark and checking described organic solvent is buffer reagent, toluene, kerosene, alcohol, acetone or chloroform.
13, Yeast Nucleic Acid as claimed in claim 11 is characterized in that as the method for product anti-fake mark and checking described inorganic solvent is a water.
14, Yeast Nucleic Acid as claimed in claim 12 is characterized in that as the method for product anti-fake mark and checking described buffer reagent is the Tris-EDTA buffer reagent.
15, Yeast Nucleic Acid as claimed in claim 1 is characterized in that as the method for product anti-fake mark and checking the method for described polymerase chain reaction amplification Yeast Nucleic Acid is to be the single or multiple polymerase chain reaction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001075802A CN1187455C (en) | 2000-05-18 | 2000-05-18 | Method of using ribonucleic acid as product antifake mark and for verification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001075802A CN1187455C (en) | 2000-05-18 | 2000-05-18 | Method of using ribonucleic acid as product antifake mark and for verification |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1324955A true CN1324955A (en) | 2001-12-05 |
| CN1187455C CN1187455C (en) | 2005-02-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB001075802A Expired - Lifetime CN1187455C (en) | 2000-05-18 | 2000-05-18 | Method of using ribonucleic acid as product antifake mark and for verification |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103262100A (en) * | 2010-11-11 | 2013-08-21 | 天使游戏纸牌股份有限公司 | Token coin for play and system for determining authenticity of token coin for play |
| CN103898218A (en) * | 2014-04-01 | 2014-07-02 | 中国海洋大学 | Molecular internal standard substance for identifying authenticity of objects and application of molecular internal standard substance |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101831489B (en) * | 2009-03-11 | 2013-10-02 | 孙星江 | Method for detecting desoxyribonucleic acid anti-counterfeiting maker by utilizing loop-mediated isothermal amplification technology |
-
2000
- 2000-05-18 CN CNB001075802A patent/CN1187455C/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103262100A (en) * | 2010-11-11 | 2013-08-21 | 天使游戏纸牌股份有限公司 | Token coin for play and system for determining authenticity of token coin for play |
| CN103898218A (en) * | 2014-04-01 | 2014-07-02 | 中国海洋大学 | Molecular internal standard substance for identifying authenticity of objects and application of molecular internal standard substance |
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| Publication number | Publication date |
|---|---|
| CN1187455C (en) | 2005-02-02 |
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Owner name: PHLICS HOLDING CO., LTD. Free format text: FORMER OWNER: BOWEI BIOLOGICAL SCIENCE +. TECHNOLOGY CO., LTD. Effective date: 20050701 |
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Effective date of registration: 20050701 Address after: The British Virgin Islands of Tortola Patentee after: Bowei Biological Sci-Tech Co., Ltd. Address before: 1, building 8, No. three, 87 Middle Road, Tu Cheng Road, Taipei City, Taiwan Province Patentee before: Bowei Biological Science &. Technology Co., Ltd. |
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