CN1323535A - Enzymolysis separation process to prepare superfine deodorized soybean product and its use - Google Patents
Enzymolysis separation process to prepare superfine deodorized soybean product and its use Download PDFInfo
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- CN1323535A CN1323535A CN 00107727 CN00107727A CN1323535A CN 1323535 A CN1323535 A CN 1323535A CN 00107727 CN00107727 CN 00107727 CN 00107727 A CN00107727 A CN 00107727A CN 1323535 A CN1323535 A CN 1323535A
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Abstract
The invention relates to soybean protein product without the smell of fish with ultramicro granularity be proteases enzymolysis. The products, including solution and powder, are made from mixed pure natural plant proteases. The products not only contain various small molecular active polypeptide and amino acid of protein that is easy for human body to absorb, but also reserves multivitamin and nutriments that ware contained in the soy protein. Such method is suitable for commercial production.
Description
The present invention relates to a kind of enzymolysis and separate superfine deodorized soy protein products, adopt and mix the technology that pure natural plant enzyme enzymolysis separates superfine deodorized soybean protein, this separates superfine deodorized soy protein products can further be mixed with oral liquid, capsule, soft capsule, dripping pill, beverage and other nutrient and healthcare products.
Enzymolysis provided by the invention separates superfine deodorized soy protein products, contains micromolecule active polypeptide and amino acid that human body very easily absorbs, also contains niacin, calcium, phosphorus, iron, magnesium, cellulose and Cobastab, B1, B2, B12.
The invention provides a kind of method that above-mentioned enzymolysis separates superfine deodorized soy protein products for preparing, i.e. method of separating superfine deodorized soybean protein with pure natural plant mixed enzyme enzymolysis.
The present invention also provides this enzymolysis to separate the purposes of superfine deodorized soy protein products.
Enzymolysis provided by the invention separates the pure-natural biological material that superfine deodorized soy protein products contains micromolecule active polypeptide and comprises the several amino acids of taurine.The weight ratio of amino acid and water can be respectively 0.014-0.8: 1 and 0.014-0.8: 0-0.05.Enzymolysis separates that water content is decided by drying means in the superfine deodorized soybean protein.
Enzymolysis of the present invention separates and contains organic nitrogen 0.2-15% in the superfine deodorized soy protein products.It is 0.2-1.5% that enzymolysis separates superfine deodorized soy protein products organic nitrogen, and enzymolysis separates and contains organic nitrogen in the superfine deodorized soybean protein dry powder is 4-15%.
Enzymolysis of the present invention separates and contains organic nitrogen 0.2-15% in the superfine deodorized soy protein products.Enzymolysis separates and contains organic nitrogen in the superfine deodorized soy protein products solution is 0.2-1.5%, and enzymolysis separates and contains organic nitrogen in the superfine deodorized soybean protein dry powder is 4-15%.
Enzymolysis of the present invention separates and contains organic molecular weight distribution in the superfine deodorized soy protein products is 50000-30, and the recommendation molecular weight is 10000-40, and wherein that molecular weight＞1000 is 20-50%, molecular weight＜1000 be 80-50%.
Enzymolysis of the present invention separates in the superfine deodorized soy protein products typical organic molecule amount and distributes as shown in Figure 1.
Enzymolysis of the present invention separates in the superfine deodorized soy protein products and contains amino acid, component is as shown in table 1, and it is as shown in table 1 that enzymolysis separates in the superfine deodorized soybean protein amino acid.
Enzymolysis of the present invention separates in the superfine deodorized soy protein products also can contain microelements of calcium, phosphorus, iron, magnesium, niacin, cellulose and Cobastab, B1, B2, B12.
Table 1 enzymolysis separates amino acid content in the superfine deodorized soy bean proteinous soln
| Amino acid | Content (mg/ml) | Amino acid | Content (mg/ml) |
| Taurine | 0.05-0.20 | Methionine | 0.82-3.58 |
| Aspartic acid | 1.15-5.55 | Isoleucine | 0.66-3.96 |
| Threonine | 0.94-3.94 | Leucine | 0.49-8.26 |
| Serine | 1.00-5.35 | Tyrosine | 0.13-2.43 |
| Glutamic acid | 1.77-8.9 | Phenylalanine | 1.23-4.43 |
| Proline | 0.62-2.44 | Ornithine | 1.13-3.43 |
| Glycine | 0.62-3.86 | Lysine | 1.02-6.47 |
| Alanine | 0.76-3.69 | Histidine | 0.42-2.20 |
| Cystine | 0.65-2.54 | Tryptophan | 0.11-2.52 |
| Valine | 1.10-5.94 | Arginine | 0.67-3.43 |
Enzymolysis separate superfine deodorized soy bean proteinous soln through 12NHCL 110 ℃ of enzymolysis 20 hours, analyze with the 835-50 of Hitachi type amino-acid analyzer again.
Ultrapure destinking soybean protein solution is at the analysis collection of illustrative plates of high-performance liquid chromatograph for enzymolysis separates for Fig. 1, and chromatographic column adopts α-25 (sepnadex) sephadex; Buffer solution: TMS-HS10, IM7.4 flow: 1ml/min, rotating speed: 0.1cm/min; AVFS:1.0.
204,1374,5000 is the standard molecular weight reference among the figure.Abscissa is the time, and ordinate is a labelled amount.
Enzymolysis of the present invention separates the preparation method of ultrapure destinking soybean protein goods, adopt and separate ultrapure destinking soybean protein, the water that adds 1-4 times of weight, reacted 6-48 hour under 30-70 ℃ with the pure natural plant mixed enzyme, stir enzymolysis, described mixed enzyme is reagent protease, papain, bromelin, lipoprotein enzyme etc., and the weight ratio of the two is 1: 1.0-1.
Can carry out when pH value 5-9 during enzymolysis, after enzyme is finished, adopt the inactivation treatment of heating, usually being heated to boils gets final product, cooled and filtered, and getting solution is that enzymolysis separates superfine deodorized soy bean proteinous soln, also can carry out sterilization treatment in case of necessity. This solution through concentrated, spray-drying enzymolysis separate ultrapure destinking soybean protein goods.
Separate superfine deodorized soy protein products through animal experiment and crowd's test with enzymolysis of the present invention. Proof can more effective angiocardiopathy preventing, be conducive to anti-oxidant, prevention breast cancer, reduction cholesterol, the prevention carcinoma of the rectum, prevention Hip Fracture, prevent climacteric scorching, improve immunity, shift in transfer and the distant place of prevention kidney trouble, prevent liver cancer, prevention prostate cancer, inhibition cancer cell, improve a poor appetite, sexual desire, improve sleep, prevent flu, suppress virus; Can promote wound healing to intestines and stomach and surgery patients; To the anaemia patient, constipation patient, old many sick, body degenerative, cell tissue is decreased the wounded obvious rehabilitation effect.
The present invention does not just replenish the needs of human nutrition, but various bioactivators in the human body (such as enzyme activation, biological oxidation) are played and activate and facilitation. This also is different from other animal protein bacterialprotease, mould amount enzyme, the method of animal pancreatin enzymolysis, but separate superfine deodorized soybean protein with pure natural plant enzyme enzymolysis, way with pure natural plant enzyme enzymolysis, this is very important progress, and this soybean with Chinese characteristics that is the Chinese pure natural plant of utilization is made separates the active peptides of superfine deodorized hydrolysate of soybean protein gained. So the present invention can be used for various health foods, comprises health drink, oral liquid, capsule, electuary, tablet, dripping pill etc.
Method of the present invention, select the various plants mixed enzyme to carry out enzymolysis to separating superfine deodorized soybean protein, has narrow spectrum popularity than single enzyme enzymatic isolation method of planting, protease is cut more thorough, the protein that separates superfine deodorized soybean protein is decomposed or various micromolecule active polypeptide and amino acid, and can preserve complete separate superfine deodorized soybean protein contained with various pure natural vitamins and mineral matter etc., having the plurality of health care functions goods has no side effect, and be a kind of easy and lower-cost enzymolysis production process, the enzyme preparation that adopts also all has no side effect, ADI does not need particular provisions (food additives, revised edition in 1985, light industry publishing house) be a kind of industrial method that is suitable for.
To help to understand the present invention by following examples of implementation, but be not limited to content of the present invention.
Example 1:
Take by weighing and separate superfine deodorized soybean protein 1000g, add distilled water 1500g, the enzyme 10g of bromelin, papain amount of reagent, stirring reaction is after 10-15 hour in the time of 20-50 ℃, be heated to and boil, cool off, filter, behind pressure sterilizing, promptly obtain enzymolysis and separate superfine deodorized soy bean proteinous soln I.This solution appearance is a light brown yellow, and blue or green fragrance is arranged, and recording the machine nitrogen content by China's pharmacopeia method is 1-0.6%, and pH value is 5.8 ± 0.5.
This enzymolysis separates superfine deodorized soy bean proteinous soln and surveys molecular weight distribution with G-25 (sephadex) column chromatography method, with be respectively 204,1347 and 5000 standard molecular weight reference, among result fruit Fig. 1, wherein molecular weight＜1000 accounts for 70%, molecular weight＞1000 account for 30%, show it is a polypeptide and an amino acid whose mixture based on micromolecule active polypeptide.
Example 2:
Change the bromelin of example into 108 papains, all the other conditions are identical, obtain the enzymolysis content solution.
Example 1 is separated superfine deodorized soy bean proteinous soln with 2 enzymolysis, and after 20 hours, analysis of amino acid is as shown in table 2 through 12NHCI, 110 ℃ of hydrolysis.
| Amino acid | Implement 2 content mg/ml | Implement 1 content mg/ml | Amino acid | Implement 2 content mg/ml | Implement 1 content mg/ml |
| Taurine | ????0.05 | ????0.11 | Methionine | ????0.82 | ????1.58 |
| Aspartic acid | ????2.94 | ????4.69 | Isoleucine | ????1.66 | ????2.76 |
| Threonine | ????1.24 | ????2.10 | Leucine | ????2.49 | ????4.26 |
| Serine | ????1.40 | ????2.35 | Tyrosine | ????0.13 | ????0.43 |
| Glutamic acid | ????3.77 | ????5.9l | Phenylalanine | ????1.23 | ????1.42 |
| Proline | ????0.62 | ????1.14 | Ornithine | ????1.43 | ????2.43 |
| Glycine | ????1.02 | ????1.66 | Lysine | ????2.02 | ????3.47 |
| Alanine | ????1.76 | ????2.81 | Histidine | ????0.62 | ????1.20 |
| Cystine | ????2.05 | ????0.54 | Tryptophan | ????0.31 | ????0.52 |
| Valine | ????2.05 | ????3.21 | Arginine | ????0.67 | ????0.43 |
Example 1 total amino acid content is 43mg/ml, and example 2 total amino acid contents are 26mg/ml, and it is pale yellow powder that the enzymolysis that makes separates superfine deodorized soybean protein freeze-dried powder.
Example 3:
1000g is separated superfine deodorized soybean protein, add 1000g distilled water, reagent protease 5-10g, papain, bromelin 8-12g, fully stir enzyme, advance reaction after 12 hours at 40 ℃ of PH6-7, be heated to and boil, cold filtration, bottle after getting the clear liquid sterilization treatment, its amino acid percentage component and seralbumin percentage composition (practical biochemistry, the first volume, 1961,81 People's Health Publishers) comparative result is as shown in table 3.Table 3 enzymolysis separates superfine deodorized soybean protein and seralbumin amino acid percentage group compares
| Amino acid | Enzymolysis separates superfine deodorized soybean protein | Seralbumin |
| Taurine | ????0.26 | ????- |
| Aspartic acid | ????10.9 | ????8.5 |
| Threonine | ????4.9 | ????5.9 |
| Serine | ????5.5 | ????5.8 |
| Glutamic acid | ????13.7 | ????14.1 |
| Proline | ????2.7 | ????4.2 |
| Glycine | ????3.8 | ????1.7 |
| Alanine | ????6.5 | ????5.1 |
| Cystine | ????1.3 | ????5.4 |
| Valine | ????7.5 | ????5.0 |
| Methionine | ????3.7 | ????1.1 |
| Isoleucine | ????6.4 | ????2.5 |
| Leucine | ????9.9 | ????10.0 |
| Tyrosine | ????1.0 | ????4.6 |
| Phenylalanine | ????3.3 | ????5.8 |
| Ornithine | ????5.6 | ????- |
| Lysine | ????8.0 | ????10.5 |
| Histidine | ????2.8 | ????3.9 |
| Tryptophan | ????1.2 | ????0.8 |
| Arginine | ????1.0 | ????4.9 |
| Amount to | ????99.96％ | ????99.8％ |
| Must amino acid | ????44.9％ | ????41.6％ |
| Branched-chain amino acid/ArAA | ????5.53 | ????1.68 |
Example 4:
The enzymolysis of embodiment 1,2,3 is separated superfine deodorized soy bean proteinous soln, spray-drying or freeze drying after concentrating separate superfine deodorized soybean protein dry powder with enzymolysis, are faint yellow powder, record that wherein to contain organic nitrogen content be 4.5-15%, moisture content is 0-2%.
Claims (7)
1, a kind of enzymolysis that contains small-molecular peptides, amino acid, multivitamin and niacin, cellulose and calcium, iron, phosphorus, magnesium separates superfine deodorized soy protein products, it is characterized in that containing the organic nitrogen of 0.2-15%, organic matter molecular mass is 50000-30, and wherein molecular weight 〉=1000 account for 20-50%.Molecular weight＜1000yhk80-50%, the weight ratio of amino acid and water is 0.014-0.8: 1-0.
2; a kind of enzymolysis as claimed in claim separates superfine deodorized soy protein products; its feature contains following amino acid: taurine 0.05-0.20mg/ml; aspartic acid 1.15-5.55mg/ml; threonine 0.94-3.94mg/ml; glycine 0.62-3.86mg/ml; alanine 0.76-3.09mg/ml; cystine 0.65-0.54mg/ml; valine 1.10-5.34mg/ml, methionine 0.82-3.58mg/ml, isoleucine 0.66-3.96mg/ml; leucine 0.49-8.26mg/ml; tyrosine 0.13-2.43mg/ml, phenylalanine 1.23-4.42mg/ml, ornithine 1.13-3.43mg/ml; lysine 0.2-6.47mg/ml; histidine 0.42-2.20mg/ml, tryptophan 0.11-2.52mg/ml, arginine 0.67-3.43mg/ml.
3, a kind ofly separate superfine deodorized soy protein products, it is characterized in that containing niacin, calcium, phosphorus, iron, magnesium, cellulose, Cobastab, B1, B2, B12 as right 2 described enzymolysis.
4, a kind of enzymolysis as claimed in claim 1 separates the preparation method of superfine deodorized soy protein products, it is characterized in that the superfine deodorized soybean protein of separation is added 1-4 times of water gaging, with the plant mixed enzyme when 30-70 ℃ and the pH value 5-9, stirring 6-48 hour mixed enzyme of enzymolysis is reagent enzyme, papain, bromelin, lipoprotein enzyme etc., the two weight ratio is 1: it is 1000-10000 that 0.1-1, every gram separate the required enzyme unit of superfine deodorized soybean protein.
5, a kind of preparation method who separates superfine deodorized soy protein products with the described enzymolysis of claim 4 is characterized in that cooling behind the solution ebuillition of heated of enzymolysis, filters.
6, a kind of enzymolysis as claimed in claim 5 separates the preparation method of superfine deodorized soybean protein, it is characterized in that through freeze drying or spray-drying.
7, a kind of purposes of separating superfine deodorized soy protein products as claim 1,2,3,4,5 or 6 described enzymolysis is characterized in that being used for health products, nutriment, functional food.
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| CN 00107727 CN1323535A (en) | 2000-05-12 | 2000-05-24 | Enzymolysis separation process to prepare superfine deodorized soybean product and its use |
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| CN00107390.7 | 2000-05-12 | ||
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004056980A1 (en) * | 2002-12-23 | 2004-07-08 | Beijing Food Research Institute | Activating protease of oil grains and hydrolyzing vegetable proteins |
| CN102115774A (en) * | 2010-11-30 | 2011-07-06 | 华东理工大学 | Method for preparing plant polypeptide by enzyme process |
| CN101704877B (en) * | 2009-11-30 | 2012-06-27 | 哈尔滨美佳娜生物工程有限责任公司 | Method for preparing soybean peptide |
| CN105613941A (en) * | 2015-12-24 | 2016-06-01 | 珠海天香苑生物科技发展股份有限公司 | Compound protein powder easy to absorb and preparation method thereof |
-
2000
- 2000-05-24 CN CN 00107727 patent/CN1323535A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004056980A1 (en) * | 2002-12-23 | 2004-07-08 | Beijing Food Research Institute | Activating protease of oil grains and hydrolyzing vegetable proteins |
| CN101704877B (en) * | 2009-11-30 | 2012-06-27 | 哈尔滨美佳娜生物工程有限责任公司 | Method for preparing soybean peptide |
| CN102115774A (en) * | 2010-11-30 | 2011-07-06 | 华东理工大学 | Method for preparing plant polypeptide by enzyme process |
| CN102115774B (en) * | 2010-11-30 | 2013-07-10 | 华东理工大学 | Method for preparing plant polypeptide by enzyme process |
| CN105613941A (en) * | 2015-12-24 | 2016-06-01 | 珠海天香苑生物科技发展股份有限公司 | Compound protein powder easy to absorb and preparation method thereof |
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