CN1316508A - BIORADAR system for optimizing fermentation procedure and amplifying data - Google Patents

Abstract

A BIORADAR system for optimazing fermentation procedure and amplifying data is composed of fermentor, upstream and downstream computers, and software package which includes main thread, sampling thread, control thread for process analysis, alarm thread and storage thread. It can increase fermentation unit by 20-60%.

Description

Be used for the BIORADAR system that optimizing fermentation and data are amplified

The present invention relates to control the computer control system of zymotechnique, be specifically related to a kind of bioprocess that is used for optimizing fermentation and data amplification and detect (BIORADAR) system.

Fermentor tank has critical role in the biotechnology industry process, and the microbial fermentation that is carried out in the fermentor tank comes down to take place on three different directions of transmission characteristic of the metabolism adjusting of hereditary property, cell levels at molecular level and engineering level regulation and control.The complicacy of fermenting process and all multifactor and multicapacity process features such as highly non-linear make system have dynamic and unpredictability.Fermenting process will reach the target of process optimization and data amplification, and the support of sophisticated Fermentation Process of Parameter detection technique must be arranged.Therefore, on lab scale, pilot scale and production-scale fermentor tank, must dispose a large amount of transmitters, various parameters such as materialization and biology are carried out online or off-line measurement, could provide abundant active data for process analysis and optimal control.FUS-50L (A) automatically controlled fermentor (seeing the separate case patent application) has been established solid basis for realizing above-mentioned functions.

But it is far from being enough that light has reliable and stable hardware.The various automatically controlled fermentors that are used for process study will have the high performance software system of a cover to go to command it to finish various tasks.The complicacy of fermenting process makes one of exploitation adapt to multiple reactor characteristics, merges various procedural theories and control theory, is convenient to the software package of fermentation process technique analysis and Optimizing operation, becomes a problem that presses for solution.

At present, the many fermentor tank control software on the market are carried out secondary development with the configuration language mostly.As the InTouch of U.S. Wonderware company, the LABTECH of LTC company, the LabView of National Instrument company, the configuration language such as Genie of magnificent company are ground in the Onspect of GCG company and Taiwan, generally all have configuration advantage easily.The open architecture may that they provide allows the user to exchange the function that (DDE) CLIENT PROGRAM is expanded original program by dynamic link libraries (DLL) or the dynamic data of writing oneself.Carrying out fermentor tank control software design with the configuration language has advantages such as programming is easy, but its opening is very much limited, and handiness is restricted to a certain extent, is difficult to carry out the function expansion by oneself hope fully.For example the AFS-Biocommand software of U.S. NEW BRUNSWICKSCIENTIFIC CO.INC. company and laboratory ferment tank arrangement; Germany B.Braun company, its software is based on parameter detecting that is used for NBS fermentor tank and cell cultures and the Controlling System of developing under the Windows system.But these devices all are based on bioprocess kinetics and design for basic optimum control operating point, therefore relevant transmitter configuration, secondary instrument, computer control system and the application software that provides is that data variation to operating point shows, storage and record, can not do further curvilinear correlation analysis, therefore can only rest on experimental implementation based on artificial experience.

The objective of the invention is to notion and theory, be developed as and realize leavened prod process optimization and be used for the computer control system that industrial data are amplified according to cellular metabolism stream and fermentor tank streams.

The present invention is at first based on to the complicacy of fermenting process, all multifactor and multicapacity process features such as highly non-linear, make system have the understanding of dynamic and unpredictability, when optimizing fermentation and amplification, be not unique method, and adopted data driven type method based on the gatherer process data to set up mathematical model.

The microbial fermentation that it is considered herein that in the fermentor tank to be carried out comes down to take place on three different levelss of transmission characteristic of the metabolism adjusting of hereditary property, cell levels at molecular level and engineering level regulation and control, and the problem of therefore distinguishing above three different levelss in the fermenting process is the major objective of data driven type method.

The present invention further proposes the cellular metabolism material again and flows the correlation properties that caused bio-reactor streams changes, be above-mentioned three the horizontal problems of research, and the substance of the data-optimized and amplification of implementation procedure, proposed the various research methods of Fermentation Process of Parameter curvilinear correlation, and in the software of native system establishment, embodied.

The invention provides the BIORADAR system that is used for optimizing fermentation and data amplification.BIORADAR of the present invention system is made of fermentor tank, host computer, next bit computer and software package system, and wherein the software package system is by control thread, the warning thread of main thread, sampling thread, process analysis with store thread and form.

There are following characteristics in BIORADAR of the present invention system:

1. the present invention has considered the various possibility situations in the fermentation process technique analysis, develop one and adapted to multiple reactor characteristic, merge the software package of various procedural theories and control theory, and design, transmitter configuration, secondary instrument and the computer control system (shown in Fig. 1 fermenting process TT﹠C system) of the fermentor tank of corresponding differing capacities.

Each sequence number is represented 1 temperature sensor respectively among Fig. 1; The 2pH transmitter; 3 dissolved oxygens (DO) transmitter; 4 complete jars of (containing material) weighing transmitters; 5 tail gas CO ₂Interface; 6 tail gas O ₂Interface; 7 tachogenerators; 8 pressure transmitters; 9 froth breaking transmitters; 10 thermal mass flow sensorses; 11 high precision peristaltic pumps; 12 matrix feed supplement electronic scaless; 13 precursors or oily electronic scales; 14 soda acid thing electronic scaless; 15 recycle pumps; 16 magnetic valves; 17 D/As; 18 A/D converters; 19 lower computers; 20 upper computers; 21 modulator-demodulator units; 22 material bottles; 23 rare-earth electric motors; 24 preheaters.

Lower computer is that bus type is the industrial computer (P II 266 4.0GB64MB) of Compact PCI, and upper computer is that PCI assembles compatible PC (P II 366 6.4GB 64MB), communicates by the PCI network interface card between the upper and lower computer.Lower computer carries out Detection ﹠ Controling to fermenting process, and the parameter of detection has leavening temperature T, dissolved oxygen concentration DO, pH, fermentating liquid volume V, mixing speed Rprn, power of agitator A, air flow quantity F, exhaust oxygen level EO ₂With carbon dioxide content ECO ₂Simultaneously, applying electronic claims technology, the feed supplement total amount and the feed rate of real time monitoring fermenting process.From T, DO, pH, V, Rpm, A and the F that transmitter comes out,, and, all be the standard signal of 4-20mA from the various signals that electronic scale comes from gas analyzer next EO2 and ECO2.Their unified A/D modules that is transported to.Lower computer is obtained after the data by network delivery to upper computer software BIORADAR system from the A/D module, according to deviation leavening temperature is carried out closed-loop control simultaneously, and feed rate is carried out open loop control.

Used performance graph analysis during fermentation process technique is analyzed is divided into direct parameter, indirect parameter and tests manual parameter.The selection configuration of picture curve has the range ability change, the selection of sample and change, the selection of curve color, the selection of background colour, functions such as the selection of coordinate axis.When many pictures dispose, different lot numbers history and real time data curve ratio are arranged, become the absolute relative time of batch fermentation, different time courses, the functions such as selection of time and time precision indirectly.

2. the componentization system of software

The BIORADAR system employs COM mechanism of Microsoft realized interaction between master routine and each sub-routine having realized data gathering, process analysis and functions such as control and warning respectively.

Shown in the componentization system of Fig. 2 BIORADAR system, 2-1 EXCEL is the product of Microsoft company, 2-2 BIORADAR system employs it with lower computer communication; 2-3 MATLAB is the mathematics manipulation software of Mathswork company, and the BIORADAR system controls according to its analysis operation result; 2-4 ALARM is a program of carrying out concrete actuation of an alarm according to the instruction of BIORADAR system.

By defining each functional module (parts) and the interface thereof in the BIORADAR system well, and allow between these parts separate, but satisfy binary code level compatibility and can utilize, interoperability, interface protocol by a standard, thereby accomplish the independence of programming language, to the minimum dependency of platform, the Version Control problem when having solved software upgrading.As long as interface is constant, each module can be upgraded separately and do not needed whole the recompility, and makes the BIORADAR system of design to integrate with other outstanding application software well, realizes the mutual interchange of data under the isomerous environment.The software design system of componentization is by design part and use the framework application of parts therein, accomplished the code reuse ability of height, and the system that makes has very big flexible degree of freedom.Parts and framework application can standalone upgrades and be need not whole recompilities, from and improved the maintainability of system.The peculiar low coupling of componentization system structure has reduced the complexity of BIORADAR system design, has improved the reliability of system, and this has special value for the very high BIORADAR system of reliability requirement.

3. the multithreaded architecture of software

The BIORADAR system will realize multitasks such as user interface, data gathering, process analysis and control and warning simultaneously.Contact very closely arranged between each task, having plenty of parallel carrying out, as the demonstration and the data gathering of user interface; What have exists precedence relationship, as advanced line data collection, then the data that collect is analyzed, if any restarting warning or the like unusually.Coordinate, get well these tasks synchronously, general programming technique is difficult to accomplish.The BIORADAR system has utilized the multithreading ability of 32-bit Windows operating system, its each major function is arranged in respectively realizes (shown in the multithreaded architecture of Fig. 3 BIORADAR system) in the different thread objects.

The BIORADAR system by 3-1 main thread (Main Thread), 3-2 sampling thread (Sample Thread), 3-3 process analysis and control thread (Control Thread), 3-4 warning thread (Alarm Thread) and 3-5 storage thread (Save Thread) totally five threads form.Wherein, main thread is responsible for the establishment and the demonstration of user interface; The sampling thread is pressed sample frequency from the load module image data; Process analysis with control thread the data that collect are analyzed and by controlled frequency to the slave computer software transfer control instruction; The warning thread starts alerter service according to abnormal conditions and reports to the police; Storing thread then is responsible for data storing such as online data and anomalous events to database, so that the use of inquiry and analysis and third party application.Because the importance of each task is difference to some extent, the execution priority of five threads just has the branch of height, sampling thread and process analysis and control thread have high priority, and main thread is common right of priority, and warning thread and storage thread then are lower right of priority.

First thread of setting up when main thread is the startup of BIORADAR system, it mainly is responsible for the establishment and the demonstration of user interface.The application program master interface that main thread is set up as shown in Figure 4.

After having set up main interface, the user can open an already present lot number or create a new lot number, begins image data then and carries out various analyses.

The sampling thread is obtained the flow process of real time data shown in Fig. 5 upper and lower computer data landline from EXCEL.

The sampling thread is as follows from the flow process that Excel receives data: void_fastcall SampleThread ∷ GetData (bool change) { OleSuccess=false; For (iht i=0; I＜5; I++) { try { CoInitialize (NULL); Excel=Variant ∷ CreateObject (＂ Excel.Application ＂); Excel. OlePropertySet (＂ Visible ＂, visible); WorkBooks=excel. OlePropertyGet (＂ WorkBooks ＂); Xian=workBooks. OleFunction (＂ Open ＂, excelFileName, update); Variant name=＂ Sheetl ＂; Sheet=xian.OlePropertyGet (＂ WorkSheets ＂, name); Range=sheet.OlePropertyGet (＂ Range ＂, address); Variant result; Result=range.OlePropertyGet (＂ Value ＂); (result.IsNull () ‖ result.IsEmpty ()); If (valid)

AnsiString?string=result.?AsType(varString);

string.?Trim();

if(！string.IsEmpty())?{

OleRemrn=string.ToDouble();

OleSuccess=true;

break;

} } Sleep(200); continue; } catch(…){

ReConnect(); Sleep(200); }?}}

The warning thread is responsible for starting alerter service according to abnormal conditions and is carried out the remote multi-media warning, and it has lower execution priority.When the sampling thread detects when unusual, abnormal conditions are write the anomalous event database, then by api function SetEvent with the set of hAlarmEvent incident.After the warning thread of waiting for is seen the set of hAlarmEvent incident, at once to alerter service send warning message WM ALARM:void _ fastcall AlarmThread ∷ Execute () while (! Terminated) { if (WAIT_OBJECT_0=WaitForSingleObject (hAlarmEvent, 1000)) { try{

m_bAlarmFinished=false;

HWND hAlarm=FindWindow (NULL, ＂ alarm center ＂),

if(hAlarm==NULL)?{

CreateProcess(NULL,＂alarm.exe＂,NULL,NULL,false,NORMAL_PRIORITY_CLASS,NULL,NULL,m_siAlarm,&m_piAlarm);

WaitForInputIdle(m_piAlarm.hProcess,2000);

HAlarm=FindWindow (NULL, ＂ alarm center ＂);

}

DWORD?result;

SendMessageTimeout(hAlarm,WM_ALARM,0,0,SMTO_BLOCK,120 ^*1000,&result); _finally{

m_bAlarmFinished=true; } }?}}

The warning thread checks at first whether alerter service starts, if do not have, then starts it, sends a message WM ALARM who defines jointly then to alerter service in TT﹠C system and alerter service, and waits in two minutes and returning.

The storage thread is responsible for a data and anomalous event is stored into database, and it has lower execution priority.When the sampling thread detects the input data buffer length greater than set(ting)value or when having anomalous event to take place, respectively with hSaveEvent and the set of hSaveAlarmEvent incident.The storage thread of waiting for see begin to carry out after hSaveEvent or the set of hSaveAlarmEvent incident storing operation: void _ fastcall SaveThread ∷ Execute () while (! Terminated)

if(WAIT_OBJECT_0=WaitForSingleObject(hSaveEvent,100)){

try{

Call(SaveOnlineData);

}

catch(…){

MessageBox(NULL,＂Exception?when?saving?online?data＂,?＂Error＂,MB_OK);

} } if(WAIT_OBJECT_0=WaitForSingleObject(hSaveAlarmEvent,100)){

try{

Call(SaveAlarmData);

}

catch(…){

MessageBox(NULL,＂Exception?when?saving?alarm?event＂,?＂Error＂,?MB_OK);

} } }

The storage thread calls SaveData () respectively according to incident set situation and SaveAlarmData () method is saved in online data and anomalous event in online database and the event database.

Although some TT﹠C software has the 32-bit Windows interface in the market, still be single-threaded structure basically.When the user needed the processing of intensive, CPU was taken by this processing for a long time, caused long-time internal procedure to can not get control or system to not response of user.Adopted after the multithreading, BIORADAR system operation is level and smooth, response in time, and sampling thread and process analysis and control thread have higher execution priority, fully guaranteed the real-time of sampling and process control.Along with plummeting of CPU price, two CPU or many CPU computer will become the industrial computer main flow, and the performance of multithreading TT﹠C software will have bigger raising.

4. the good man computer interface of software

There is multiple good man computer interface in the BIORADAR system, comprises establishment, demonstration and the printing of graphics view and tabular views, establishment of various the configuration dialog boxs and demonstration etc.

The A graphics view

The BIORADAR system provides dynamically and static two kinds of graphics views.Dynamic view is an on-line parameter and the real-time demonstration of the indirect parameter relevant with on-line parameter, it the time ask that axle carries out along with process and adjusts automatically, its shown data is directly from the sampling thread.Static view has realized the diagrammatic representation of historical data.It according to specified time period of user and parameter name from online and (or) the off-line data database retrieve data, calculate indirect parameter, with curve form various parameters are shown then.In the static view, the user can change the time period and the parameter that will show at any time, both can observe overall phenomenon (long-time section), also can study local circumstance's (short time period).

In addition, graphics view also has functions such as the bound of curve filtering, baseline elimination, the selection of curve color and curve display is provided with.Be the interface of graphics view as shown in Figure 6.Wherein DO is the on-line measurement value, and CER is online calculated value, and CU is the off-line measurement value, and RCU is the calculated off-line value.Various types of parameters are presented on the same picture, for industrial analysis and optimization provide great convenience.

The code of configuration graphics view time period and parameter name is as follows: TSempDlg ^*Dlg=new TSetupDlg (this, data_tb); Dlg-＞StepMaskEdit-＞Enabled=false; Dlg-＞StepComboBox-＞Enabled=false; Int flag=dlg-＞ShowModal (); If (IDOK=flag)

MakeName();

start=data_tb-＞from;

end=data_tb-＞to;

DataLine? ^*line;

int?figureCount=myfigure-＞Count();

for(int?i=0;i＜figureCount;i++)

int?penColor=0,smooth;

float?max,min;

penColor=info-＞on-＞Get(name-＞Strings[i]).lineColor;

max=info-＞on-＞Get(name-＞Strings[i]).maxValue;

min=info-＞on-＞Get(name-＞Strings[i]).minValue;

smooth=info-＞on-＞Get(name-＞Strings[i]).smoothInterval;

line=new?DataLine(name-＞Strings[i],false,max,min,penColor);

line-＞SetDrawTime(start,end);

if(WAIT_OBJECT_0=WaitForSingleObject(hViewMutex,5000)){

int?arrayNumber=viewArray-＞Count;

for(int?j=0;j＜arrayNumber;j++){

float?value=( ^*viewArray)[j][index];

unsigned intcurrentTime=((double)( ^*viewArray)[j].time-(double)beginTime) ^*60 ^*24;

DataPoint ^*a=new?DataPoint(currentTime,value);

if(！a)?continue;

bool?result=line-＞Add(a);

if(！result)

delete?a;

}

}

ReleaseMutex(hViewMutex);

line-＞SetSmoothInterval(smooth);

line-＞SetFullName(fullName-＞Strings[i]);

myfigure-＞Add(line); }

if(！haveAdd?&&?oldStart＜=start?&&?oldEnd＞=end)

RedrawItemClick(this); }

if(dlg)

delete?dlg；

Configuration graphics view time period and parameter name are shown in Fig. 7 graphics view time period and parameter name configuration interface.

The code that the bound of selection of graphics view curve color and curve display is provided with is as follows: TColorDlg ^*Dlg=new TColorDlg (Application, color_tb, myfigure);

im?flag=dlg-＞ShowModal();

if(IDOK=flag){

DataLine&?line=myfigure-＞Get(color_tb-＞name);

line.SetPenColor(color_tb-＞color);

line.SetMax(color_tb-＞max,true);

line.SetMin(color_tb-＞min,true);

line.SetSmoothInterval(color_tb-＞smooth);

Draw();

}

The interface that the bound of selection of graphics view curve color and curve display is provided with as shown in Figure 8.

It is as follows that the coordinate axis of graphics view is provided with code: TAxisDlg ^*Dlg=new TAxisDlg (this, axis_tb);

int?flag=dlg-＞ShowModal();

XAxis&?xaxis=myfigure-＞GetXAxis();

YAxis&?yaxis=myfigure-＞GetYAxis();

if(IDOK=fiag){

xaxis.SetLabel(axis_tb-＞xtitle);

xaxis.SetUnit((TimeUnit)axis_tb-＞unit);

yaxis.SetLabel(axis_tb-＞ytitle);

axisColor=axis_tb-＞color;

axisPen-＞Color=colors[axisColor];

xaxis.SetPenColor(axisColor);

yaxis.SetPenColor(axisColor);

Draw();

}

The coordinate axis of graphics view is provided with the interface as shown in Figure 9.

B database and tabular views

For the ease of data utilization and analysis, simultaneously also for the ease of third party's routine access, the BIORADAR system all is saved in the data of all data of program generation and user's input in the Paradox database.The BIORADAR system has set up three databases: online database is preserved the data of online acquisition, and offline database is preserved the data of user's off-line input, the variety of event that event database takes place when then stored routine moves.

There are three kinds of tabular views in the BIORADAR system, be respectively applied for three databases demonstration and (or) revise.Online tabular views (as shown in figure 10) but parameter changing conditions that both real-time follow-up was relevant with on-line parameter also can set by certain hour and show part or all of on-line parameter value in certain time period at interval according to the user.By off-line tabular views (as shown in figure 11), the user can import or edit the offline parameter value.What kind of incident when the incident tabular views have take place to user report, and is out-of-limit as a certain parameter, and a certain equipment opens or closes etc.

C prints

Various graphics views and tabular views all can be printed at any time.Both can directly print to printer, also can Print to File earlier, print again at the appropriate time then.Graphics view is printed in the What You See Is What You Get mode.During printing reports, the user can be provided with the options such as time period, parameter name and the timed interval of printing.

5. the fermentor device with native system configuration must have temperature, mixing speed, ventilation flow rate, tank pressure, froth breaking, pH, DO fermented liquid true volume or weight, three feed supplement amounts, tail gas CO ₂And O ₂Deng the detect parameters more than 14, can realize system configuration (figure-1) with FUS-50L (A) automatically controlled fermentor, realize the purpose of laboratory ferment process optimization.Native system and the configuration of industrial fermentation jar can realize the data-optimized of fermentation industry process and amplify purpose.

6. native system and fermentor device supporting after, the relevant fermenting process high density data that native system is gathered can reach remote data transmission, realizes strange land process analysis and diagnosis, realizes the analysis expert of multi-specialized subject.

BIORADAR of the present invention system is for realizing optimizing fermentation and the data amplification condition that provides great convenience.Its a plurality of versions, at the different scales automatically controlled fermentor in the optimization of erythromycin, penicillin and duomycin fermentation with amplify in the research and use.Native system and FUS-50L (A) automatically controlled fermentor is supporting, will have the very big market, estimates that annual value of production is more than 1,000 ten thousand.Native system and various technical scale fermentor tank are supporting, realize the data-optimized of fermentation industry process and amplify purpose, from the test-results that has obtained, penicillin fermentation improves unit 40%, abomacetin fermentation improves unit 65%, penicillin fermentation improves unit 30%, improves the industrial production benefit altogether more than 5,000 ten thousand.

Embodiment: 1, operating requirement

80386 above types, the above internal memory of 8M, the above free hard disk space of 20M proves the softdog or the key floppy disc of user identity, Windows 9x, Windows NT 3.5 or the Chinese and western language operating system of highest version are more finished the slave computer software of concrete data gathering and are started with 5.1

From explorer select bioradar.exe or start menu, input bioradar.exe in service place catalogue bioradar, by starting bioradar.exe behind the enter key, insert boot disk (if boot disk is arranged) through prompting, program will be activated, and main window as shown in figure 12 occur.2, lot number

In the bioprocess detection system, lot number is basic unit.The test that every beginning is once new is with generation～individual new lot number.It by a lot number configuration file ( ^*.cfg file), two databases (online database, offline database) are formed, and each database is by one or several ^*.db and ^*.px file is formed.

Create a new lot number (1) and select " new lot number " down or press " new lot number " button at tool bar from master menu " file ", " the new lot number name " dialog box (2) that occurs is as shown in figure 13 accepted in " new lot number name " or change lot number title.If use the lot number configuration of the overall situation, jump to (4).(3) configuration lot number.(4) from master menu " sampling ", select " beginning " or select " beginning to sample " button at tool bar.(5) at this moment, a new lot number generates.Can create various views by master menu " view ".Attention: when is sampling when a lot number (1), can not create other new lot numbers.When (2) naming to lot number, proposal name length does not surpass 13 English characters.

Close lot number

Select " closing this lot number " down from master menu " file ", close all windows and the file of current lot number.Attention: when (1) lot number was being sampled, this lot number can not be closed.Should stop to close lot number again after the sampling.When (2) creating or opening another lot number, current lot number Saves and Closes automatically.

Preserve lot number

Select " preserving this lot number " down from master menu " file ", the configuration of current lot number is saved in ^*.cfg in the file.

Open an already present lot number (1) and select " opening lot number " down or select " opening lot number " button, with " opening lot number " dialog box that occurs as shown in figure 14 at tool bar from master menu " file ".(2) from " opening lot number " dialog box, select a lot number title, by opening button.(3) at this moment, lot number is opened, can or select " beginning " or select " beginning to sample " button resampling at tool bar from master menu " sampling " by master menu " view " the various views of establishment.。Attention:

When a lot number is being sampled, can not open other lot number.2.1 configuration lot number

Default password: none.

System configuration

In the bioprocess detection system, system configuration is the integral part of lot number.System configuration has been stipulated title, input range and the smooth length of various parameters.In addition, system configuration has also defined password, default coordinate axis and other miscellaneous option etc.(1) from master menu " sampling " selection " configuration ", with the system configuration dialog that occurs as shown in figure 15.(2) select one from the name list frame, press property button (in the name list frame, double-click a certain and can open properties dialog fast).According to the type of selected parameter, on-line parameter, offline parameter or the properties dialog ejection of parameter indirectly.By these dialog boxes, can dispose selected parameter.(3) press the password button, can dispose password.(4) press the coordinate axis button, can dispose coordinate axis.(5) press miscellaneous button, can dispose miscellaneous.(6) press the lower frequency button, can configuration frequency.(7) press from the online button that is provided with, can determine the metering system of air flow quantity and biological fluid volume.Attention: (1) if the current lot number of not creating or opening, all changes will act on the lot number that after this all create as global configuration, but to former already present lot number without any influence.(2) if existing lot number is opened or created, the change of being made only acts on current lot number.2.2 frequency allocation (1) selects " frequency " of " system configuration " to order down from master menu " sampling ", with the frequency allocation dialog box that occurs as shown in figure 16.(2) input sample frequency numerical value.(3) select sample unit.2.3 select down " from the online setting " order of " system configuration " from online setting (1) from master menu " sampling ", with occur as shown in figure 17 from the Configuration Online dialog box.(2) determine that respectively air flow quantity and biological fluid volume are online acquisition or off-line input, and the initial value of input off-line input.3, sampling 3.1 begins sampling

Select " " down or select " beginning sampling " button from tool bar from master menu " sampling ", the thread of sampling afterwards just begins sampling by the sample frequency of setting.Attention: (1) lot number has begun after the sampling, and " beginning sampling " order can not reuse.(2) begin after the sampling, before stopping sampling, current lot number can not be closed.(3) before beginning sampling, please guarantee the EXCEL that does not open in the operating system.If can not be sure of, can press the Ctrl+Alt+Del triple bond simultaneously and open " bolt down procedure " dialog box, and check whether EXCEL is wherein arranged.If EXCEL is arranged, please select EXCEL, close opened EXCEL by " ending task " button then, in " bolt down procedure " dialog box, no longer include EXCEL till.(4) in the program samples process, please don't open EXCEL voluntarily.3.2 suspend sampling

Select " time-out " down or select " suspending sampling " button from master menu " sampling ", suspend sampling at tool bar.Attention: (1) begins before the sampling, and " suspending sampling " order can not be used.(2) suspend after the sampling, please use " restart sampling " order recovers to sample.3.3 recover sampling

Select " restarting " down or press " restarting sampling " button from master menu " sampling ", recover the sampling that has suspended at tool bar.Attention: before suspending sampling, " restarting sampling " order can not be used (1).3.4 stop sampling

Select " stopping " down or press " stopping sampling " button from master menu " sampling ", stop sampling at tool bar.Attention: (1) is suspended after the sampling, and before recovering sampling, " stopping sampling " order can not be used.(2) begin after the sampling, before stopping sampling, current lot number can not be closed.4, graphics view 4.1 is created motion graphics view (1) and is selected " dynamic view " order down from master menu " view ".(2), the variety of option of view is set by the context menu of master menu " figure " or this view.Attention: (1) begins can not create the motion graphics view before the sampling.(2) suspend or stop the sampling after, motion graphics changes no longer in time.Select " static attitude view " order 4.2 create static graphics view (1) down from master menu " view ".(2), the variety of option of view is set by the context menu of master menu " figure " or this view.From the context menu of master menu " figure " or view, select " configuration " order 4.3 change the configuration (1) of graphics view, with the configuration dialog box that occurs as shown in figure 18.(2) " from " and " to " input or time range and the unit of selecting view to show, if selected " relative value " check box, " to " time with respect to " from " time.(3) by double-clicking the parameter that the selection of " selecting " and " can select " list box will show.4.4 the options such as color (1) of parameter are selected " color " order in the change graphics view from the context menu of master menu " figure " or view, with the change ColorDialog that occurs as shown in figure 19.(2) parameter name that selection will change from the name list frame.(3) change color, maximum value, minimum value and smoothness, if necessary.(4) press application button, make and revise effectively.(5) repeat 2-4, the parameter that change will change.From the context menu of master menu " figure " or view, select " coordinate axis " order 4.5 change the coordinate axis (1) of graphics view, with the configuration coordinate axis dialog box that ejects as shown in figure 20.(2) title of input X-axis and Y-axis in X-axis title and Y-axis title.(3) unit of selection X-axis in X-axis unit.(4) color of selection coordinate axis in the coordinate axis color.From the context menu of master menu " figure " or view, select " miscellaneous " order 4.6 change miscellaneous (1) of graphics view, with the miscellaneous the configuration dialog box that ejects as shown in figure 21.(2) from " background colour ", select the view background color.(3) from " legend look ", select the legend color.4.7 renewal view

Select " repainting " order from the context menu of master menu " figure " or graphics view, when online or offline database changed, " repainting " order can reflect these changes.Attention:

" repainting " order only can use in the static graphics view.

The display cycle (1) that changes graphics view is selected " cycle " order from the context menu of master menu " figure " or graphics view, with the cycle the configuration dialog box that ejects as shown in figure 22.(2) input display cycle numerical value.Attention:

This order only can be used for the motion graphics view.5, data base view 5.1 is created online data base view (1) and is selected " online form " order down from master menu " view ".(2) the context menu configuration database by master menu " database " or this view.5.2 configuration online database (1) is selected " configuration " order from the context menu of master menu " database " or this view, with the configuration dialog box that ejects as shown in figure 23.(2) " from " and " to " input or time range and the unit of selecting view to show, if selected " relative value check box, " to " time with respect to " from " time.(3) by double-clicking the parameter that the selection of " selecting " and " can select " list box will show.(4) select to show step-length by " step-length ".5.3 be automatically updated in the line data base view

Select " upgrading automatically " order from the context menu of master menu " database " or this view, the online database view will change in time and upgrade automatically.5.4 the online data base view of manual renewal

Select " the manual renewal " order from the context menu of master menu " database " or this view, the online database view will upgrade once by hand.5.5 create offline database view (1) from master menu " view " selection " off-line form " down.(2) by master menu " database " configuration database.Attention: (1) only can create an offline database.

Insert one and record offline database

Select " insertion " order down from master menu " database ", with the off-line data input table that occurs as shown in figure 24.

Press confirming button after the input data, new record is added to offline database.Record in the offline database is by the records in automatic ordering 5.6 editor's offline databases of time

Select " editor " order down from master menu " database ".5.7 the record in the deletion offline database

Select " deletion " order, the current record in the delete database down from master menu " database ".6, printing 6.1 Print Previews (1) selects " Print Preview " to order or press " Print Preview " button on the tool bar down from master menu " file ".(2) if working as frontview is data base view, print preview window will appear.If graphics view, because the screen finding is the printing gained, so the Print Preview order does not realize.(3) the Close button in click data the library preview window withdraws from the preview window or Print button and begins to print (as shown in figure 25).6.2 printing " printing " button of selecting " printing " to order or press on the tool bar down from master menu " file " begins to print when the frontview content.6.3 software package gained curve legend

Certain gene engineering product from June 16th, 99 begin to June 24 order end, carry out the high-density high expression level and cultivate, to all on-line parameters, offline parameter gained curve (as shown in figure 26).

Claims (7)

1, a kind of BIORADAR system that is used for optimizing fermentation and data amplification, it is characterized in that this system is made up of fermentor tank, host computer, next bit computer and software package system, wherein the software package system is made of control thread, warning thread and the storage thread of main thread, sampling thread, process analysis.

2, a kind of BIORADAR system that optimizing fermentation and data are amplified that is used for according to claim 1 is characterized in that wherein said main thread is responsible for the establishment and the demonstration of user interface; The sampling thread is pressed sample frequency from the load module image data; Process analysis with control thread the data that collect are analyzed and by controlled frequency to the slave computer software transfer control instruction; The warning thread starts alerter service according to abnormal conditions and reports to the police; Storing thread then is responsible for data storing such as online data and anomalous events to database, so that the use of inquiry and analysis and third party application, because the importance of each task is difference to some extent, the execution priority of five threads just has the branch of height, sampling thread and process analysis and control thread have high priority, main thread is common right of priority, and warning thread and storage thread then are lower right of priority.

3, a kind of BIORADAR system that is used for optimizing fermentation and data amplification according to claim 1, it is characterized in that there is multiple man-machine interface in this system, comprise establishment, demonstration and the printing of graphics view and tabular views, establishment of various the configuration dialog boxs and demonstration.

4, a kind of BIORADAR system that is used for optimizing fermentation and data amplification according to claim 1, it is characterized in that the BIORADAR system has set up three databases: online database is preserved the data of online acquisition; Offline database is preserved the data of user's off-line input; The variety of event that event database takes place when then stored routine moves.

5, a kind of BIORADAR system that is used for optimizing fermentation and data amplification according to claim 1, it is characterized in that there are three kinds of tabular views in the BIORADAR system, be respectively applied for the demonstration and the modification of three databases, the online tabular views parameter changing conditions that both but real-time follow-up was relevant with on-line parameter, also can set by certain hour and show part or all of on-line parameter value in certain time period at interval according to the user, by the off-line tabular views, the user can import or edit the offline parameter value, what kind of incident when the incident tabular views have take place to user report, out-of-limit as a certain parameter, a certain equipment opens or closes.

6, a kind of BIORADAR system that is used for optimizing fermentation and data amplification according to claim 1, it is characterized in that wherein said fermentor tank has temperature, mixing speed, ventilation flow rate, tank pressure, froth breaking, pH, DO fermented liquid true volume or weight, three feed supplement amounts, tail gas CO ₂And O ₂Deng the detect parameters more than 14.

7, a kind of BIORADAR system that is used for optimizing fermentation and data amplification according to claim 1 is characterized in that this system can realize the remote transmission of data.

Images