CN1313491C - Cat omega interferon and its coding gene and uses - Google Patents
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Abstract
The present invention discloses cat omega interferon, a coded gene thereof and an application thereof. The cat omega interferon is a protein with one of the following amino acid residue sequences: 1) SEQ ID No. 1 in a sequence list, 2) SEQ ID No. 2 in the sequence list, 3) SEQ ID No. 3 in the sequence list, 4) SEQ ID No. 4 in the sequence list, 5) SEQ ID No. 5 in the sequence list, 6) SEQ ID No. 6 in the sequence list, 7) SEQ ID No. 7 in the sequence list, 8) SEQ ID No. 8 in the sequence list, 9) SEQ ID No. 9 in the sequence list, 10) SEQ ID No. 10 in the sequence list, 11) SEQ ID No. 11 in the list, 12) SEQ ID No. 12 in the sequence list, 13) SEQ ID No. 13 in the sequence list, and 14) a protein which is used for substituting, deleting or adding one to ten amino acid residues of the amino acid residue sequences of SEQ ID No. 1 to SEQ ID No. 13 and has the anti-infection function for feline leukemia viruses, FIV and canine parvovius viruses.
Description
Technical field
The present invention relates to albumen and encoding gene thereof and application, particularly relate to cat omega interferon and encoding gene thereof and its application in the anti-infectives of preparation feline leukaemia virus, FIV and canine parvovius.
Background technology
Interferon, rabbit is that cell induces action-reaction to virus infection or the various synthetic biology that reaches, and produces the protein molecular of the natural generation of a class of justacrine, and molecular weight is 15,000-21,000 dalton.The interference of Que Dinging have two big classes: I type and II type.I type Interferon, rabbit comprises IFN-α, IFN-β, IFN-ω and four hypotypes of IFN-τ, and II type Interferon, rabbit mainly contains IFN-γ.
1992, (the Toray Industries of toray Co., Ltd., people such as Nakamura Inc.) have been separated to feline interferon gene (Molecular cloning of feline interferon cDNA by directexpression.Biosci Biotechnol Biochem.1992 first, 56 (2): 211-4), and it is ranged ω type Interferon, rabbit (Homogenous production of feline interferon in silkworm byreplacing single amino acid code in signal peptide region in recombinantbaculovirus and characterization of the product.J.Vet.Med.Sci.1993,55 (2): 251-8) in 1993.1994, based on its recombinant interferon medicine Intercat
In Japan's listing, and be used to treat cat calicivirus infection and canine parvovius infection.After this, cat IFN-α, IFN-β and IFN-γ are split in succession.But, the article that people such as Ramani Wonderling in 2002 deliver shows, cat ω type interferon gene and cat alpha-interferon dna homolog that people such as Nakamura are separated to reach 94-99% (Cloning, expression, purification, and biological activity of five feline type Iinterferons.Vet Immunol Immunopathol.2002,89 (1-2): 13-27).And, people such as Roberts RM had carried out defining (New and atypical families oftype I interferons in mammals:comparative functions to the difference of IFN-α and IFN-ω in 1997, structures, andevolutionary relationships.Prog Nucleic Acid Res Mol Biol.1997,56:287-325), difference mainly contains 2 points: first, IFN-ω has the conserved amino acid sequence of being made up of six amino-acid residues at the C end, and this sequence is at IFN-α, all do not exist among IFN-β and the IFN-τ; The second, the position and the number of proline(Pro) have high conservative, and IFN-α is holding the 4th, 26 from N, and 39,110 and 139 exist conservative proline(Pro), and IFN-ω shortage is held the 139th proline(Pro) from N.The cat ω type interferon gene that people such as Nakamura are separated to neither possesses the conserved amino acid sequence that IFN-ω C end should have, and holding the 142nd to have proline(Pro) from N, therefore think that in fact the cat ω type interferon gene that people such as Nakamura is separated to should be cat alpha-interferon gene.
Summary of the invention
The purpose of this invention is to provide a cat omega interferon and encoding gene thereof.
Cat omega interferon provided by the present invention derives from domestic cat (Felis sivestris), is the protein with one of amino acid residue sequence of following cat omega interferon family:
1) the SEQ ID № in the sequence table: 1;
2) the SEQ ID № in the sequence table: 2;
3) the SEQ ID № in the sequence table: 3;
4) the SEQ ID № in the sequence table: 4;
5) the SEQ ID № in the sequence table: 5;
6) the SEQ ID № in the sequence table: 6;
7) the SEQ ID № in the sequence table: 7;
8) the SEQ ID № in the sequence table: 8;
9) the SEQ ID № in the sequence table: 9;
10) the SEQ ID № in the sequence table: 10;
11) the SEQ ID № in the sequence table: 11;
12) the SEQ ID № in the sequence table: 12;
13) the SEQ ID № in the sequence table: 13;
14) with SEQ ID № in the sequence table: the amino acid residue sequence of 1-13 is through replacement, disappearance or the interpolation of one to ten amino-acid residue and feline leukaemia virus, FIV and canine parvovius are had the protein of anti-infectious function.
Have SEQ ID № in the sequence table: the albumen of 1-13 amino acid residue sequence can be distinguished called after FeIFN-ω 1 to FeIFN-ω 13, SEQ ID № in the sequence table: 1-11 is made up of 196 amino-acid residues, and SEQ ID №: 12-13 is made up of 203 amino-acid residues.
The encoding gene of above-mentioned cat omega interferon is selected from one of nucleotide sequence of following cat omega interferon family:
1) SEQ ID № in the sequence table: 14 dna sequence dna;
2) SEQ ID № in the sequence table: 15 dna sequence dna;
3) SEQ ID № in the sequence table: 16 dna sequence dna;
4) SEQ ID № in the sequence table: 17 dna sequence dna;
5) SEQ ID № in the sequence table: 18 dna sequence dna;
6) SEQ ID № in the sequence table: 19 dna sequence dna;
7) SEQ ID № in the sequence table: 20 dna sequence dna;
8) SEQ ID № in the sequence table: 21 dna sequence dna;
9) SEQ ID № in the sequence table: 22 dna sequence dna;
10) SEQ ID № in the sequence table: 23 dna sequence dna;
11) SEQ ID № in the sequence table: 24 dna sequence dna;
12) SEQ ID № in the sequence table: 25 dna sequence dna;
13) SEQ ID № in the sequence table: 26 dna sequence dna;
14) nucleotide sequence of the dna sequence dna hybridization that under the rigorous condition of height, can limit with SEQ ID №: 14-26 in the sequence table;
15) SEQ ID № in the code sequence tabulation: 1 proteic dna sequence dna;
16) SEQ ID № in the code sequence tabulation: 2 proteic dna sequence dnas;
17) SEQ ID № in the code sequence tabulation: 3 proteic dna sequence dnas;
18) SEQ ID № in the code sequence tabulation: 4 proteic dna sequence dnas;
19) SEQ ID № in the code sequence tabulation: 5 proteic dna sequence dnas;
20) SEQ ID № in the code sequence tabulation: 6 proteic dna sequence dnas;
21) SEQ ID № in the code sequence tabulation: 7 proteic dna sequence dnas;
22) SEQ ID № in the code sequence tabulation: 8 proteic dna sequence dnas;
23) SEQ ID № in the code sequence tabulation: 9 proteic dna sequence dnas;
24) SEQ ID № in the code sequence tabulation: 10 proteic dna sequence dnas;
25) SEQ ID № in the code sequence tabulation: 11 proteic dna sequence dnas;
26) SEQ ID № in the code sequence tabulation: 12 proteic dna sequence dnas;
27) SEQ ID № in the code sequence tabulation: 13 proteic dna sequence dnas;
The rigorous condition of described height can be that (or 0.1 * SSC), the solution of 0.1%SDS is hybridized and washed film with 0.1 * SSPE under 65 ℃.
Have SEQ ID № in the sequence table: the gene of 14-26 polynucleotide sequence can be distinguished called after FeIFN-ω 1 to FeIFN-ω 13, SEQ ID № in the sequence table: 14-24 is by 591 based compositions, its encoding sequence is that coding has SEQ ID № in the sequence table respectively: the protein of 1-11 amino acid residue sequence from 5 ' end 1-the 591st bit base.SEQ ID № in the sequence table: 25-26 is by 612 based compositions, and its encoding sequence has SEQ ID № in the sequence table for encoding respectively from 5 ' end 1-the 612nd bit base: the protein of 12-13 amino acid residue sequence.
Contain in expression carrier of the present invention, transgenic cell line, host bacterium and the amplification cat omega interferon encoding gene arbitrary segmental primer to all belonging to protection scope of the present invention.
Another object of the present invention provides a kind of method of expressing above-mentioned cat omega interferon.
The method of the above-mentioned cat omega interferon of expression provided by the present invention is that the recombinant expression vector that will contain above-mentioned cat omega interferon encoding gene imports host cell, expresses obtaining cat omega interferon.
But described host is the protokaryon or the eukaryotic cell of arbitrary expression alien gene.
Described prokaryotic cell prokaryocyte can be colibacillus, as E.coli DH5 α, E.coli TB1 or E.coli BL21DE3 etc.
Described eukaryotic cell can be yeast cell, mammalian cell and vegetable cell etc., comprises yeast SMD1168H, yeast GS115, yeast X-33, yeast KM71H, COS-7, CHO or BHK-21 etc.
The carrier that sets out that is used to make up the recombinant expression vector that contains the cat omega interferon encoding gene can be pLLp-STII (U.S.'s gene Dynamic Test Chamber), pPICZ α A (U.S. invitrogen company), pET carrier series, pQE carrier series, pPIC9K or pPICZ α.
Be the carrier that sets out with pLLp-STII, the recombinant expression vector that contains described disintegrin gene of structure is FeIFN-ω/pLLp-STII.
Be the carrier that sets out with pPICZ α A, the recombinant expression vector that contains described disintegrin gene of structure is FeIFN-ω/pPICZ α A.
When described host is colibacillus, need to add IPTG and carry out abduction delivering, add IPTG concentration be 0.2-1.0mM, be preferably 0.2mM.
When described host is yeast, need to add methyl alcohol and carry out abduction delivering, add methyl alcohol final concentration be 0.1-1.0%, be preferably 0.5%.
Cultivation contains the substratum and the culture condition of the host cell of disintegrin encoding gene of the present invention, all can be substratum and the culture condition of cultivating the host that sets out.
The present invention also provides the anti-infectives of a kind of feline leukaemia virus, FIV virus and canine parvovius.
Anti-infectives provided by the present invention, its activeconstituents is above-mentioned cat omega interferon.
When needing, in above-mentioned anti-infectives, can also add one or more pharmaceutically acceptable carriers.Described carrier comprises thinner, vehicle, weighting agent, tackiness agent, wetting agent, disintegrating agent, absorption enhancer, tensio-active agent, absorption carrier of pharmaceutical field routine etc.
Medicine of the present invention can be made various ways such as injection liquid, tablet, pulvis, granula, capsule, oral liquid.The medicine of above-mentioned various formulations all can be according to the ordinary method preparation of pharmaceutical field.
The consumption of said medicine is generally the 1-2.5MU/kg body weight, successive administration or administration every other day, and be 3-7 days the course of treatment.
The homology of the amino acid residue sequence of feline interferon provided by the present invention and sheep and people's ω type Interferon, rabbit is respectively 64% and 58%.And its aminoacid sequence possesses six conserved amino acid sequences that IFN-ω C end should have, and does not have proline(Pro) simultaneously hold the 139th amino acids residue from N near, possesses the feature of ω type Interferon, rabbit.Cat omega interferon of the present invention and encoding gene thereof are expected to be prepared into the anti-infectives of clinical feline leukaemia virus, FIV and canine parvovius, will play a significant role in the control of Animal diseases.
Description of drawings
Fig. 1 is the SDS-PAGE detected result of the e. coli expression product of cat ω type interferon gene
Fig. 2 is the SDS-PAGE detected result of the yeast expression product of cat ω type interferon gene
Fig. 3 is the SDS-PAGE detected result of purified reorganization cat ω type Interferon, rabbit
Embodiment
Method therefor is ordinary method if no special instructions among the following embodiment.All primers synthesize and examining order is finished by the living worker in Shanghai.And experiment shows that in all following experiments, the result of 13 kinds of different cat omega interferon subtype gene is consistent.
The clone of embodiment 1, cat omega interferon gene
1, design of primers
According to the conservative amino acid residues sequence of Interferon, rabbit family, and design a pair of primer with reference to the human omega interferon gene order, primer sequence is as follows:
P1：5’-atggccctcctgctccctct-3’
P2：5’-tcaagatgassccaggtctccatc-3’
2, the extraction of the total RNA of cat spleen
Get the about 200mg of cat spleen tissue that suffers from acute peritonitis, transfer in the glass grinding device after shredding rapidly, add 2mLTrizol reagent (Invitrogen company), organize mixing to be placed on 15min on ice, grind 10-15 time, make the abundant cracking of splenocyte with spleen.Then, the Trizol-homogenation of spleen tissue is transferred in the 1.5mL RNase-free centrifuge tube, added the chloroform of 1/5 homogenate volume, violent mixing 15sec, centrifugal 15min.Get supernatant, add and the isopyknic Virahol of supernatant precipitation at room temperature 10min, centrifugal 10min again.Abandon supernatant, with 75% ethanol centrifuge washing precipitation once, precipitation places air to dry.At last, add 30 μ l distilled waters (RNase-free) dissolving RNA precipitation, promptly get the total RNA of cat spleen.
3, RT-PCR amplification cat omega interferon gene
The total RNA of cat spleen with extraction is a template, under the guiding of primer P1 and primer P2, utilize QIAGEN one-stepRT-PCR test kit (QIAGEN company) and reference reagent box specification sheets to carry out the amplification of cat omega interferon gene, reaction conditions is: 50 ℃ of 30min at first; Next 94 ℃ of 10min; 94 ℃ of 30sec once more, 50 ℃ of 30sec, 72 ℃ of 1min, totally 35 circulations; Last 72 ℃ of 5min.With the PCR product in 4 ℃ of preservations.
4, order-checking and sequential analysis
Target DNA subclone about the about 600bp of the size of pcr amplification is gone among the T carrier pGEM-T (promega company), picking mono-clonal order-checking after screening, sequencing result shows and obtains 13 kinds of different Interferon, rabbit subtype gene sequences altogether, has the SEQ ID № in the sequence table respectively: the nucleotide sequence of 14-26, SEQ ID № in the sequence table: 14-24 is by 591 based compositions, its encoding sequence is that coding has SEQ ID № in the sequence table respectively: the protein of the amino acid residue sequence of 1-11 from 5 ' end 1-the 591st bit base; SEQ ID № in the sequence table: 25-26 is by 612 based compositions, and its encoding sequence is that coding has SEQ ID № in the sequence table respectively: the protein of the amino acid residue sequence of 12-13 from 5 ' end 1-the 612nd bit base.The amino acid residue sequence of institute's isolated genes amino acids coding residue sequence and sheep and people's ω type Interferon, rabbit is carried out homology relatively, with the homology of sheep ω type Interferon, rabbit be 64%, with people ω type Interferon, rabbit homology be 58%, the amino acid sequence homology of Interferon, rabbit that people such as Nakamura are separated to and sheep ω type Interferon, rabbit is 49%, with the amino acid sequence homology of people ω type Interferon, rabbit be 53%.And the interferon gene encoding amino acid sequence that is separated to possesses six conserved amino acid sequences that IFN-ω C end should have, and does not have proline(Pro) simultaneously hold the 139th amino acids residue from N near, possesses the feature of ω type Interferon, rabbit.
The expression of embodiment 2, cat ω type interferon gene and the purifying of expression product thereof
One, expression and the fermentative production of cat ω type interferon gene in intestinal bacteria
1, the structure that contains the coli expression carrier FeIFN-ω/pLLp-STII of cat ω type interferon gene
The plasmid that will contain 13 kinds of different cat ω type Interferon, rabbit subtype gene sequences respectively with restriction enzyme BamHI and HindIII double digestion after subclone go in the carrier pLLp-STII of same enzyme double digestion, the prokaryotic expression plasmid that makes up is referred to as FeIFN-ω/pLLp-STII, transformed into escherichia coli DH5 α.Cut through PCR and enzyme and to identify that the back obtains positive reorganization bacterium, the amplification cultivation engineering bacteria, the adding final concentration is the inductor IPTG of 0.2mM, induces under 30 ℃ 3 hours.After cultivating end, expression product is carried out the SDS-PAGE electrophoresis detection, (swimming lane M is low molecular weight protein (LMWP) Marker to detected result as shown in Figure 1, swimming lane 1 is an expression product), about 19kD, locate to occur a protein band, conform to expected results, show the correctly expression in intestinal bacteria of cat ω type interferon gene.
2, the fermentative production of reorganization cat omega interferon
Picking step 1 obtains positive colony list bacterium colony, and it is inoculated in 100 milliliters of LB liquid nutrient mediums that contain 100 mg/ml penbritins, and 37 ℃ of shake-flask culture 12 hours are as primary seed solution; With 1% inoculum size primary seed solution is transferred in the fermentor tank that contains 6 liters of LB liquid nutrient mediums again, under the condition identical, cultivate and cause OD with step 1
600Value is 1.0.
Cold shock is handled fermented liquid: after the fermentation ends, and centrifugal collection thalline, with containing 30mM Tris-HCl, the quality percentage composition is 20% sucrose, the solution suspension thalline of 1mM EDTA pH8.0 (by every gram thalline 80mL), ice bath vibrated 10 minutes gently.Then, centrifugal 4 ℃ of 8000g removes supernatant, will precipitate and use 5mM MgSO
4Ice bath after suspending vibrated 10 minutes gently.Centrifugal 15 minutes of 12000g gets supernatant and is fermented liquid after cold shock is handled.
Two, expression and the fermentative production of cat ω type interferon gene in yeast
1, the structure that contains the Yeast expression carrier FeIFN-ω/pPICZ α A of cat ω type interferon gene
The plasmid that will contain 13 kinds of different cat ω type Interferon, rabbit subtype gene sequences respectively with restriction enzyme XhoI and XbaI double digestion after subclone go in the carrier pPICZ of same enzyme double digestion α A, the yeast secreted expression plasmid that makes up is referred to as FeIFN-ω/pPICZ α A, electricity transforms the SMD1168H yeast strain, transformant is laid on the YPDS that contains zeocin (Invitrogen company) 100 μ g/mL (contains 1% yeast extract, 2% yeast culture tryptone, 2% glucose, the 1M sorbyl alcohol, 2% agar) flat board, cultivated 2-3 days for 30 ℃, until growing single bacterium colony, after identifying, PCR obtains positive reorganization bacterium.The positive bacterium of recombinating is inoculated in BMGY earlier and (contains 1% yeast extract, 2% yeast culture tryptone, 100mM potassiumphosphate (pH 6.0), 1.34%YNB, 4 * 10
-5% vitamin H, 1% glycerine) in the substratum, cultivate 18h-20h, treat OD for 30 ℃
600When value was 2-6, the centrifugal 5min of 1500g collected thalline, changes the BMMY substratum again into and (contains 1% yeast extract, 2% yeast culture tryptone, 100mM potassiumphosphate (pH 6.0), 1.34%YNB, 4 * 10
-5The % vitamin H, 0.5% methyl alcohol), continue under the same conditions to cultivate to make its OD
600Reach 1, the adding final concentration was 0.5% methanol induction expression in per then 24 hours, collected a sample, and induced 48 hours for 30 ℃ in per 24 hours.After cultivating end, expression product is carried out the SDS-PAGE electrophoresis detection, (swimming lane M is low molecular weight protein (LMWP) Marker to detected result as shown in Figure 2, swimming lane 1 is an expression product), about 19kD, locate to occur a protein band, conform to expected results, show the correctly expression in yeast of cat ω type interferon gene.
2, the fermentative production of reorganization cat omega interferon
Fermentor tank is the Biostat B. of B.Braun company, and capacity is 5 liters.Concrete fermenting process is, picking step 1 obtains positive colony list bacterium colony, it is inoculated in the YPD substratum that 20mL contains 0.1 μ g/mL zeocin (contains 1% yeast extract, 2% yeast culture tryptone, 2% glucose) in, 30 ℃, 250rpm were cultivated 30 hours, inserted 200mL again and did not have in the YPD substratum of zeocin, and 30 ℃, 250rpm are cultivated 24h.After cultivating end, centrifugal collection thalline is inoculated into 2 liters of BMGY substratum with thalline and (contains 1% yeast extract, 2% yeast culture tryptone, 100mM potassiumphosphate (pH 6.0), 1.34%YNB, 4 * 10
-5% vitamin H, 1% glycerine) in, it is 5.5 times at 30 ℃, dissolved oxygen more than 30%, pH, after cultivating 15h, add 50% glycerine, flow velocity 20mL glycerine/L bacterium liquid/hour, stop to mend glycerine when wet cell weight reaches 240g/L, when dissolved oxygen significantly rises, the pH value rises, glycerine exhausts in the substratum, begins to drip 24% methanol induction and expresses flow velocity 3.6mL methyl alcohol/L bacterium liquid/hr, in 8 hours, methanol concentration is increased to 70%, and coinduction was expressed 5 days, made the cat omega interferon gene obtain to express.
Three, the purifying of reorganization cat omega interferon
Get the fermented liquid of step 1 and step 2, step 1 fermented liquid cold shock is handled back centrifuging and taking supernatant, step 2 fermented liquid 10000g, centrifugal 10min precipitation thalline, get supernatant, with the vinegar acid for adjusting pH value is 4.5, cross cation-exchange chromatography post (HiTrap SP FF, Amersham Biosciences), collect the target elution peak, after anion-exchange chromatography post (HiTrap Q FF, Amersham Biosciences), collects the target elution peak, cross Sephacryl molecular sieve chromatography (Amersham Biosciences), collect the target elution peak, obtain the reorganization cat omega interferon of purifying after the filtration sterilization, the albumen of purifying is carried out the SDS-PAGE electrophoresis detection, (swimming lane M is low molecular weight protein (LMWP) Marker to detected result as shown in Figure 3, swimming lane 1 is a purified product), about 19kD, locate to occur a protein band, conform to, show to have obtained the higher cat ω type Interferon, rabbit base of purity with expected results.
Embodiment 3, cytopathic-effect inhibition assay are identified the biological activity of reorganization cat omega Interferon, rabbit
Adopt cat embryo fibroblast and vesicular stomatitis virus (VSV) to measure antiviral the tiring of reorganization cat omega Interferon, rabbit, concrete grammar is: get conceived 15 days cat embryo, decaptitate, four limbs and internal organ, under the sterile state with PBS give a baby a bath on the third day after its birth all over after, shred.Blow and beat into last 96 orifice plates behind the individual cells, be cultured to whole adherent growth after, remove nutrient solution, divide two groups, one group of every hole adds 4 times of multiple proportions (4
0-4
11U/mL) escherichia coli expression and the purified reorganization cat omega Interferon, rabbit of embodiment 2 acquisitions of dilution, another is organized every hole and adds 4 times of multiple proportions (4
0-4
11U/mL) Pichia anomala expression and the purified reorganization cat omega Interferon, rabbit of embodiment 2 acquisitions of dilution are used 100 TCID
50The virus of dosage is attacked poison, control wells adds virus-free nutrient solution, set up negative control (only to add Interferon, rabbit simultaneously through the reorganization cat omega of doubling dilution, do not add virus), positive control (do not add Interferon, rabbit, only add virus), blank (do not add Interferon, rabbit, do not add virus), judged result in the observation control wells appearance 50% above pathology under inverted microscope.Observe after 24 hours, 100% serious pathology appears in the positive control hole, and adding has through 4
10,4
9With 4
870%, 40%, 0% pathology appears respectively in the cell hole at escherichia coli expression and purified reorganization cat omega interferon of U/mL doubling dilution, is higher than 4 in adding
-8The cell hole of concentration Interferon, rabbit, cell state is good, and the purification of Recombinant cat omega interferon of Pichia anomala expression carries out 4
11, 4
10With 4
9The cell hole that is added behind the U/mL doubling dilution has 80%, 30%, 0% pathology respectively, is higher than 4 in adding
-9The cell hole of concentration Interferon, rabbit, cell state is good.It in the negative control hole normal cell.The above results shows that the biologic activity of the reorganization cat omega Interferon, rabbit of escherichia coli expression is 4
8U/mL, the biologic activity of the reorganization cat omega Interferon, rabbit of Pichia anomala expression is 4
9U/mL.
Embodiment 4, safety experiment
1, gets the cat (male and female half and half) in 200 9 months to two years old ages, every intravenous injection 10
7U/kg reorganization cat omega Interferon, rabbit, 76 cat appearance as a result are slightly drowsiness, transference cure after 5-6 hour.25 cat body temperature slightly raise, but within normal range.Ionogen index no abnormality seen in the body fluid.
2, get 200 one-year-old beasle dogs (male and female half and half), every intravenous injection 10 to 7 years old age
7U/kg reorganization cat omega Interferon, rabbit, 5 dog appearance as a result are slightly drowsiness, transference cure after 5-6 hour.Ionogen index no abnormality seen in the body fluid.
The clinical trial result of embodiment 5, reorganization cat omega interferon
1, gets and infect 30 of the cats that leukosis virus is arranged, be divided into administration group and control group, 15 every group.The reorganization cat omega interferon of administration group subcutaneous injection every day 1MU/kg, control group is only injected placebo, injects continuously three days.The result shows that the reorganization cat omega interferon can treat feline leukaemia virus effectively and infect, and morbidity cat disease feelings are clearly better, and is embodied in to rise to normal or near normal, heating paresthesia disappears from cell number.
2, get 30 one-year-old beasle dogs (male and female half and half), be divided into administration group and control group, 15 every group to 7 years old age.Give all dog inoculation II type canine parvovirus (CPV), to the parvovirus infections symptom occurring.With the reorganization cat omega interferon of administration group intravenous injection every day 2.5MU/kg, control group is only injected placebo again, injects continuously three days.The result shows that the reorganization cat omega interferon can treat Canine parvovirus infection effectively, and morbidity dog disease feelings are clearly better, and is embodied in the white corpuscle number and rises to normal or near normal, heating, vomiting, symptom of diarrhea disappear.
Sequence table
<160>26
<210>1
<211>196
<212>PRT
＜213〉domestic cat (Felis sivestris)
<400>1
Met?Ala?Leu?Leu?Leu?Pro?Leu?Leu?Thr?Ala?Leu?Ala?Leu?Leu?Thr?Cys
1 5 10 15
Arg?Pro?Gly?Gly?Ser?Leu?Gly?Cys?Ala?Leu?Pro?Gly?Ser?His?Ala?Gln
20 25 30
Val?Ser?Arg?Asp?Asn?Leu?Val?Leu?Leu?Gly?Gln?Met?Arg?Arg?Leu?Ser
35 40 45
Pro?Phe?Leu?Cys?Leu?Arg?Ala?Arg?Lys?Asp?Phe?Arg?Phe?Pro?Arg?Glu
50 55 60
Met?Leu?Glu?Gly?Gly?Gln?Leu?Arg?Glu?Ala?Gln?Ala?Ala?Ala?Ala?Val
65 70 75 80
Leu?Arg?Glu?Leu?Leu?Gln?Gln?Thr?Phe?Asn?Leu?Leu?His?Thr?Glu?Arg
85 90 95
Ser?Ser?Ala?Ala?Trp?Ser?Pro?Ala?Pro?Leu?His?Gly?Leu?Arg?Ser?Gly
100 105 110
Leu?His?Arg?Gln?Leu?Glu?Ala?Leu?Asp?Ala?Cys?Trp?Leu?Gln?Ala?Thr
115 120 125
Gly?Glu?Gly?Glu?Arg?Ala?Pro?Gly?Met?His?Gly?Pro?Ala?Leu?Ala?Ile
130 135 140
Lys?Arg?Tyr?Phe?Gln?Asp?Ile?Arg?Val?Tyr?Leu?Glu?Asp?Glu?Gly?Tyr
145 150 155 160
Ser?Asp?Cys?Ala?Trp?Glu?Ile?Val?Arg?Leu?Glu?Ile?Met?Arg?Ala?Leu
165 170 175
Ser?Ser?Ser?Ala?Thr?Leu?Gln?Asp?Ser?Leu?Ala?Ile?Lys?Asp?Gly?Asp
180 185 190
Leu?Ala?Ser?Ser
195
<210>2
<211>196
<212>PRT
＜213〉domestic cat (Felis sivestris)
<400>2
Met?Ala?Leu?Leu?Leu?Pro?Leu?Leu?Thr?Ala?Leu?Ala?Leu?Leu?Thr?Cys
1 5 10 15
Arg?Pro?Gly?Gly?Ser?Leu?Gly?Cys?Ala?Leu?Pro?Gly?Ser?His?Ala?Gln
20 25 30
Val?Ser?Arg?Asp?Asn?Leu?Val?Leu?Leu?Gly?Gln?Met?Arg?Arg?Leu?Ser
35 40 45
Pro?Phe?Leu?Cys?Leu?Arg?Ala?Arg?Lys?Asp?Phe?Arg?Phe?Pro?Arg?Glu
50 55 60
Met?Leu?Glu?Gly?Gly?Gln?Leu?Arg?Glu?Ala?Gln?Ala?Ala?Ala?Ala?Val
65 70 75 80
Leu?Arg?Glu?Leu?Leu?Gln?Gln?Thr?Phe?Asn?Leu?Leu?His?Thr?Glu?Arg
85 90 95
Ser?Ser?Ala?Ala?Trp?Ser?Pro?Ala?Pro?Leu?His?Gly?Leu?Arg?Ser?Gly
100 105 110
Leu?His?Arg?Gln?Leu?Glu?Ala?Leu?Asp?Ala?Cys?Leu?Leu?Gln?Ala?Thr
115 120 125
Gly?Glu?Gly?Glu?Arg?Ala?Pro?Gly?Met?His?Gly?Pro?Ala?Leu?Ala?Ile
130 135 140
Lys?Arg?Tyr?Phe?Gln?Asp?Ile?Arg?Val?Tyr?Leu?Glu?Asp?Glu?Gly?Tyr
145 150 155 160
Ser?Asp?Cys?Ala?Trp?Glu?Ile?Val?Arg?Leu?Glu?Ile?Met?Arg?Ala?Leu
165 170 175
Ser?Ser?Ser?Ala?Thr?Leu?Gln?Asp?Ser?Leu?Ala?Ile?Lys?Asp?Gly?Asp
180 185 190
Leu?Ala?Ser?Ser
195
<210>3
<211>196
<212>PRT
＜213〉domestic cat (Felis sivestris)
<400>3
Met?Ala?Leu?Leu?Leu?Pro?Leu?Leu?Thr?Ala?Leu?Val?Leu?Leu?Thr?Cys
1 5 10 15
Arg?Pro?Gly?Gly?Ser?Leu?Gly?Cys?Ala?Leu?Pro?Gly?Ser?His?Ala?Gln
20 25 30
Val?Ser?Arg?Asp?Asn?Leu?Val?Leu?Leu?Gly?Gln?Met?Arg?Arg?Leu?Ser
35 40 45
Pro?Phe?Leu?Cys?Leu?Arg?Ala?Arg?Lys?Asp?Phe?Arg?Phe?Pro?Arg?Glu
50 55 60
Met?Leu?Glu?Gly?Gly?Gln?Leu?Arg?Glu?Ala?Gln?Ala?Ala?Ala?Ala?Val
65 70 75 80
Leu?Arg?Glu?Leu?Leu?Gln?Gln?Thr?Phe?Asn?Leu?Leu?His?Thr?Glu?Arg
85 90 95
Ser?Ser?Ala?Ala?Trp?Ser?Pro?Ala?Pro?Leu?His?Gly?Leu?Arg?Ser?Gly
100 105 110
Leu?His?Arg?Gln?Leu?Glu?Ala?Leu?Asp?Ala?Cys?Leu?Leu?Gln?Ala?Thr
115 120 125
Gly?Glu?Gly?Glu?Arg?Ala?Pro?Gly?Met?His?Gly?Ser?Ala?Leu?Ala?Ile
130 135 140
Lys?Arg?Tyr?Phe?Gln?Asp?Ile?Arg?Val?Tyr?Leu?Glu?Asp?Glu?Gly?Tyr
145 150 155 160
Ser?Asp?Cys?Ala?Trp?Glu?Ile?Val?Arg?Leu?Glu?Ile?Met?Arg?Ala?Leu
165 170 175
Ser?Ser?Ser?Ala?Thr?Leu?Gln?Asp?Ser?Leu?Ala?Ile?Lys?Asp?Gly?Asp
180 185 190
Leu?Gly?Ser?Ser
195
<210>4
<211>196
<212>PRT
＜213〉domestic cat (Felis sivestris)
<400>4
Met?Ala?Leu?Leu?Leu?Pro?Leu?Leu?Thr?Ala?Leu?Ala?Leu?Leu?Thr?Cys
1 5 10 15
Arg?Pro?Gly?Gly?Ser?Leu?Gly?Cys?Ala?Leu?Pro?Gly?Ser?His?Ala?Gln
20 25 30
Val?Ser?Arg?Asp?Asn?Leu?Val?Leu?Leu?Gly?Gln?Met?Arg?Arg?Leu?Ser
35 40 45
Pro?Phe?Leu?Cys?Leu?Arg?Ala?Arg?Lys?Asp?Phe?Arg?Phe?Pro?Arg?Glu
50 55 60
Met?Leu?Glu?Gly?Gly?Gln?Leu?Arg?Glu?Ala?Gln?Ala?Ala?Ala?Ala?Val
65 70 75 80
Leu?Arg?Glu?Leu?Leu?Gln?Gln?Thr?Phe?Asn?Leu?Leu?His?Thr?Glu?Arg
85 90 95
Ser?Ser?Ala?Ala?Trp?Ser?Pro?Ala?Pro?Leu?His?Gly?Leu?Arg?Ser?Gly
100 105 110
Leu?His?Arg?Gln?Leu?Glu?Ala?Leu?Asp?Ala?Cys?Trp?Leu?Gln?Ala?Thr
115 120 125
Gly?Glu?Gly?Glu?Arg?Ala?Pro?Gly?Met?His?Gly?Pro?Val?Leu?Ala?Ile
130 135 140
Lys?Arg?Tyr?Phe?Gln?Asp?Ile?Arg?Val?Tyr?Leu?Glu?Asp?Glu?Gly?Tyr
145 150 155 160
Ser?Asp?Cys?Ala?Trp?Glu?Ile?Val?Arg?Leu?Glu?Ile?Met?Arg?Ala?Leu
165 170 175
Ser?Ser?Ser?Ala?Thr?Leu?Gln?Asp?Ser?Leu?Ala?Ile?Lys?Asp?Gly?Asp
180 185 190
Leu?Gly?Ser?Ser
195
<210>5
<211>196
<212>PRT
＜213〉domestic cat (Felis sivestris)
<400>5
Met?Ala?Leu?Leu?Leu?Pro?Leu?Leu?Thr?Ala?Leu?Ala?Leu?Leu?Thr?Cys
1 5 10 15
Arg?Pro?Gly?Gly?Ser?Leu?Gly?Cys?Ala?Leu?Pro?Gly?Ser?His?Ala?Gln
20 25 30
Val?Ser?Arg?Asp?Asn?Leu?Val?Leu?Leu?Gly?Gln?Met?Arg?Arg?Leu?Ser
35 40 45
Pro?Phe?Leu?Cys?Leu?Arg?Ala?Arg?Lys?Asp?Phe?Arg?Phe?Pro?Arg?Glu
50 55 60
Met?Leu?Glu?Gly?Gly?Gln?Leu?Arg?Glu?Ala?Gln?Ala?Ala?Ala?Ala?Val
65 70 75 80
Leu?Arg?Glu?Leu?Leu?Gln?Gln?Thr?Phe?Asn?Leu?Leu?His?Thr?Glu?Arg
85 90 95
Ser?Ser?Ala?Ala?Trp?Ser?Pro?Ala?Pro?Leu?His?Gly?Leu?Arg?Ser?Gly
100 105 110
Leu?His?Arg?Gln?Leu?Glu?Ala?Leu?Asp?Ala?Cys?Leu?Leu?Gln?Ala?Thr
115 120 125
Gly?Glu?Gly?Glu?Arg?Ala?Pro?Gly?Met?His?Gly?Pro?Ala?Leu?Ala?Ile
130 135 140
Lys?Arg?Tyr?Phe?Gln?Asp?Ile?Arg?Val?Tyr?Leu?Glu?Asp?Glu?Gly?Tyr
145 150 155 160
Ser?Asp?Cys?Ala?Trp?Glu?Ile?Val?Arg?Leu?Glu?Ile?Met?Arg?Ala?Leu
165 170 175
Ser?Ser?Ser?Ala?Thr?Leu?Gln?Asp?Ser?Leu?Ala?Ile?Lys?Asp?Gly?Asp
180 185 190
Leu?Gly?Ser?Ser
195
<210>6
<211>196
<212>PRT
＜213〉domestic cat (Felis sivestris)
<400>6
Met?Ala?Leu?Leu?Leu?Pro?Leu?Leu?Thr?Ala?Leu?Ala?Leu?Leu?Thr?Cys
1 5 10 15
Arg?Pro?Gly?Gly?Ser?Leu?Gly?Cys?Ala?Leu?Pro?Gly?Ser?His?Ala?Gln
20 25 30
Val?Ser?Arg?Asp?Asn?Leu?Val?Leu?Leu?Gly?Gln?Met?Arg?Arg?Leu?Ser
35 40 45
Pro?Phe?Leu?Cys?Leu?Arg?Ala?Arg?Lys?Asp?Phe?Arg?Phe?Pro?Arg?Glu
50 55 60
Met?Leu?Glu?Gly?Gly?Gln?Leu?Arg?Glu?Ala?Gln?Ala?Ala?Ala?Ala?Val
65 70 75 80
Leu?Arg?Glu?Leu?Leu?Gln?Gln?Thr?Phe?Asn?Leu?Leu?His?Thr?Glu?Arg
85 90 95
Ser?Ser?Ala?Ala?Trp?Ser?Pro?Ala?Pro?Leu?His?Gly?Leu?Arg?Ser?Gly
100 105 110
Leu?His?Gln?Gln?Leu?Glu?Ala?Leu?Asp?Ala?Cys?Leu?Leu?Gln?Ala?Thr
115 120 125
Gly?Glu?Gly?Glu?Arg?Ala?Leu?Gly?Met?His?Gly?Pro?Ala?Leu?Ala?Ile
130 135 140
Lys?Arg?Tyr?Phe?Gln?Asp?Ile?Arg?Val?Tyr?Leu?Glu?Asp?Glu?Gly?Tyr
145 150 155 160
Ser?Asp?Cys?Ala?Trp?Glu?Ile?Val?Arg?Leu?Glu?Ile?Met?Arg?Ala?Leu
165 170 175
Ser?Ser?Ser?Ala?Thr?Leu?Gln?Asp?Ser?Leu?Ala?Ile?Lys?Asp?Gly?Asp
180 185 190
Leu?Ala?Ser?Ser
195
<210>7
<211>196
<212>PRT
＜213〉domestic cat (Felis sivestris)
<400>7
Met?Ala?Leu?Leu?Leu?Pro?Leu?Leu?Thr?Ala?Leu?Ala?Leu?Leu?Thr?Cys
1 5 10 15
Arg?Pro?Gly?Gly?Ser?Leu?Gly?Cys?Ala?Leu?Pro?Gly?Ser?His?Ala?Gln
20 25 30
Val?Ser?Arg?Asp?Asn?Leu?Val?Leu?Leu?Gly?Gln?Met?Arg?Arg?Leu?Ser
35 40 45
Pro?Phe?Leu?Cys?Leu?Arg?Ala?Arg?Lys?Asp?Phe?Arg?Phe?Pro?Arg?Glu
50 55 60
Met?Leu?Glu?Gly?Gly?Gln?Leu?Arg?Glu?Ala?Gln?Ala?Ala?Ala?Ala?Val
65 70 75 80
Leu?Arg?Glu?Leu?Leu?Gln?Gln?Thr?Phe?Asn?Leu?Leu?His?Thr?Glu?Arg
85 90 95
Ser?Ser?Ala?Ala?Trp?Ser?Pro?Ala?Pro?Leu?His?Gly?Leu?Arg?Ser?Gly
100 105 110
Leu?His?Arg?Gln?Leu?Glu?Ala?Leu?Asp?Ala?Cys?Trp?Leu?Gln?Ala?Thr
115 120 125
Gly?Glu?Gly?Glu?Arg?Ala?Pro?Gly?Met?His?Gly?Pro?Val?Leu?Ala?Ile
130 135 140
Lys?Arg?Tyr?Phe?Gln?Asp?Ile?Arg?Val?Tyr?Leu?Glu?Asp?Glu?Gly?Tyr
145 150 155 160
Ser?Asp?Cys?Ala?Trp?Glu?Ile?Val?Arg?Leu?Glu?Ile?Met?Arg?Ala?Leu
165 170 175
Ser?Ser?Ser?Ala?Thr?Leu?Gln?Asp?Ser?Leu?Ala?Ile?Lys?Asp?Gly?Asp
180 185 190
Leu?Ala?Ser?Ser
195
<210>8
<211>196
<212>PRT
＜213〉domestic cat (Felis sivestris)
<400>8
Met?Ala?Leu?Leu?Leu?Pro?Leu?Leu?Thr?Ala?Leu?Ala?Leu?Leu?Thr?Cys
1 5 10 15
Arg?Pro?Gly?Gly?Ser?Leu?Gly?Cys?Ala?Leu?Pro?Gly?Ser?His?Ala?Gln
20 25 30
Val?Ser?Arg?Asp?Asn?Leu?Val?Leu?Leu?Gly?Gln?Met?Arg?Arg?Leu?Ser
35 40 45
Pro?Phe?Leu?Cys?Leu?Arg?Ala?Arg?Lys?Asp?Phe?Arg?Phe?Pro?Arg?Glu
50 55 60
Met?Leu?Glu?Gly?Gly?Gln?Leu?Arg?Glu?Ala?Gln?Ala?Ala?Ala?Ala?Val
65 70 75 80
Leu?Gln?Glu?Leu?Leu?Gln?Gln?Thr?Phe?Asn?Leu?Leu?His?Thr?Glu?Arg
85 90 95
Ser?Ser?Ala?Ala?Trp?Ser?Pro?Val?Pro?Leu?His?Gly?Leu?Arg?Ser?Gly
100 105 110
Leu?His?Arg?Gln?Leu?Glu?Ala?Leu?Asp?Ala?Cys?Leu?Leu?Gln?Ala?Thr
115 120 125
Gly?Glu?Gly?Glu?Arg?Ala?Pro?Gly?Met?His?Gly?Pro?Ala?Leu?Ala?Ile
130 135 140
Lys?Arg?Tyr?Phe?Gln?Asp?Ile?Arg?Val?Tyr?Leu?Glu?Asp?Glu?Gly?Tyr
145 150 155 160
Ser?Asp?Cys?Ala?Trp?Glu?Ile?Val?Arg?Leu?Glu?Ile?Met?Arg?Ala?Leu
165 170 175
Ser?Ser?Ser?Ala?Thr?Leu?Gln?Asp?Ser?Leu?Ala?Ile?Lys?Asp?Gly?Asp
180 185 190
Leu?Ala?Ser?Ser
195
<210>9
<211>196
<212>PRT
＜213〉domestic cat (Felis sivestris)
<400>9
Met?Ala?Leu?Leu?Leu?Pro?Leu?Leu?Thr?Ala?Leu?Ala?Leu?Leu?Thr?Cys
1 5 10 15
Arg?Pro?Gly?Gly?Ser?Leu?Gly?Cys?Ala?Leu?Pro?Gly?Ser?His?Ala?Gln
20 25 30
Val?Ser?Arg?Asp?Asn?Leu?Val?Leu?Leu?Gly?Gln?Met?Arg?Arg?Leu?Ser
35 40 45
Pro?Phe?Leu?Cys?Leu?Arg?Ala?Arg?Lys?Asp?Phe?Arg?Phe?Pro?Arg?Glu
50 55 60
Met?Leu?Glu?Gly?Gly?Gln?Leu?Trp?Glu?Ala?Gln?Ala?Ala?Ala?Ala?Val
65 70 75 80
Leu?Arg?Glu?Leu?Leu?Gln?Gln?Thr?Phe?Asn?Leu?Leu?His?Thr?Glu?Arg
85 90 95
Ser?Ser?Ala?Ala?Trp?Ser?Pro?Ala?Pro?Leu?His?Gly?Leu?Arg?Ser?Gly
100 105 110
Leu?His?Arg?Gln?Leu?Glu?Ala?Leu?Asp?Ala?Cys?Leu?Leu?Gln?Ala?Thr
115 120 125
Gly?Glu?Gly?Glu?Arg?Ala?Pro?Gly?Met?His?Gly?Pro?Ala?Leu?Ala?Ile
130 135 140
Lys?Arg?Tyr?Phe?Gln?Asp?Ile?Arg?Val?Tyr?Leu?Glu?Asp?Glu?Gly?Tyr
145 150 155 160
Ser?Asp?Cys?Ala?Trp?Glu?Ile?Val?Arg?Leu?Glu?Ile?Met?Arg?Ala?Leu
165 170 175
Ser?Ser?Ser?Ala?Thr?Leu?Gln?Asp?Ser?Leu?Ala?Ile?Lys?Asp?Glu?Asp
180 185 190
Leu?Gly?Ser?Ser
195
<210>10
<211>196
<212>PRT
＜213〉domestic cat (Felis sivestris)
<400>10
Met?Ala?Leu?Leu?Leu?Pro?Leu?Leu?Thr?Ala?Leu?Ala?Leu?Leu?Thr?Cys
1 5 10 15
Arg?Pro?Gly?Gly?Ser?Leu?Gly?Cys?Ala?Leu?Pro?Gly?Ser?His?Ala?Gln
20 25 30
Val?Ser?Arg?Asp?Asn?Leu?Val?Leu?Leu?Gly?Gln?Met?Arg?Arg?Leu?Ser
35 40 45
Pro?Phe?Leu?Cys?Leu?Arg?Ala?Arg?Lys?Asp?Phe?Arg?Phe?Pro?Arg?Glu
50 55 60
Met?Leu?Glu?Gly?Gly?Gln?Leu?Arg?Glu?Ala?Gln?Ala?Ala?Ala?Ala?Val
65 70 75 80
Leu?Arg?Glu?Leu?Leu?Gln?Gln?Thr?Phe?Asn?Leu?Leu?His?Thr?Glu?Arg
85 90 95
Ser?Ser?Ala?Ala?Trp?Ser?Pro?Ala?Pro?Leu?His?Gly?Leu?Arg?Ser?Gly
100 105 110
Leu?His?Arg?Gln?Leu?Glu?Ala?Leu?Asp?Ala?Cys?Leu?Leu?Gln?Ala?Thr
115 120 125
Gly?Glu?Gly?Glu?Arg?Ala?Pro?Gly?Met?His?Gly?Pro?Ala?Leu?Ala?Ile
130 135 140
Lys?Arg?Tyr?Phe?Gln?Asp?Ile?Arg?Val?Tyr?Leu?Glu?Asp?Glu?Gly?Tyr
145 150 155 160
Ser?Asp?Cys?Ala?Trp?Glu?Ile?Val?Arg?Leu?Glu?Ile?Met?Arg?Ala?Leu
165 170 175
Ser?Ser?Ser?Ala?Thr?Leu?Gln?Asp?Ser?Leu?Ala?Ile?Lys?Asp?Gly?Asp
180 185 190
Leu?Gly?Leu?Ser
195
<210>11
<211>196
<212>PRT
＜213〉domestic cat (Felis sivestris)
<400>11
Met?Ala?Leu?Leu?Leu?Pro?Leu?Leu?Thr?Ala?Leu?Ala?Leu?Leu?Thr?Cys
1 5 10 15
Arg?Pro?Gly?Gly?Ser?Leu?Gly?Cys?Ala?Leu?Pro?Gly?Ser?His?Thr?Gln
20 25 30
Val?Ser?Arg?Asp?Asn?Leu?Val?Leu?Leu?Gly?Gln?Met?Arg?Arg?Leu?Ser
35 40 45
Pro?Phe?Leu?Cys?Leu?Arg?Ala?Arg?Lys?Asp?Phe?Arg?Phe?Pro?Arg?Glu
50 55 60
Met?Leu?Glu?Gly?Gly?Gln?Leu?Arg?Glu?Ala?Gln?Ala?Ala?Ala?Ala?Val
65 70 75 80
Leu?Arg?Glu?Leu?Leu?Gln?Gln?Thr?Phe?Asn?Leu?Leu?His?Thr?Glu?Arg
85 90 95
Ser?Ser?Ala?Ala?Trp?Gly?Pro?Ala?Pro?Leu?His?Gly?Leu?Arg?Ser?Gly
100 105 110
Leu?His?Arg?Gln?Leu?Glu?Ala?Leu?Asp?Ala?Cys?Leu?Leu?Gln?Ala?Thr
115 120 125
Gly?Glu?Gly?Glu?Arg?Ala?Pro?Gly?Met?His?Gly?Pro?Asp?Leu?Ala?Ile
130 135 140
Lys?Arg?Tyr?Phe?Gln?Asp?Ile?Arg?Val?Tyr?Leu?Glu?Asp?Glu?Gly?Tyr
145 150 155 160
Ser?Asp?Cys?Ala?Trp?Glu?Ile?Val?Arg?Leu?Glu?Ile?Met?Arg?Ala?Leu
165 170 175
Ser?Ser?Ser?Ala?Thr?Leu?Gln?Asp?Ser?Leu?Ala?Ile?Lys?Asp?Gly?Asp
180 185 190
Leu?Gly?Ser?Ser
195
<210>12
<211>203
<212>PRT
＜213〉domestic cat (Felis sivestris)
<400>12
Met?Ala?Leu?Leu?Leu?Pro?Leu?Leu?Thr?Ala?Leu?Ala?Leu?Leu?Thr?Cys
1 5 10 15
Arg?Pro?Gly?Gly?Ser?Leu?Gly?Cys?Ala?Leu?Pro?Gly?Ser?His?Ala?Gln
20 25 30
Val?Ser?Arg?Asp?Asn?Leu?Val?Leu?Leu?Gly?Gln?Met?Arg?Arg?Leu?Ser
35 40 45
Pro?Phe?Leu?Cys?Leu?Arg?Ala?Arg?Lys?Asp?Phe?Arg?Phe?Pro?Arg?Glu
50 55 60
Met?Leu?Glu?Gly?Gly?Gln?Leu?Arg?Glu?Ala?Gln?Ala?Ala?Ala?Ala?Val
65 70 75 80
Leu?Arg?Glu?Leu?Leu?Gln?Gln?Thr?Phe?Asn?Leu?Leu?His?Thr?Glu?Arg
85 90 95
Ser?Ser?Ala?Ala?Trp?Ser?Pro?Ala?Pro?Leu?His?Gly?Leu?Arg?Ser?Gly
115 120 125
Gly?Glu?Gly?Glu?Arg?Ala?Thr?Gly?Glu?Gly?Glu?Arg?Ala?Pro?Gly?Met
130 135 140
His?Gly?Pro?Val?Leu?Ala?Ile?Lys?Arg?Tyr?Phe?Gln?Asp?Ile?Arg?Val
145 150 155 160
Tyr?Leu?Glu?Asp?Glu?Gly?Tyr?Ser?Asp?Cys?Ala?Trp?Glu?Ile?Val?Arg
165 170 175
Leu?Glu?Ile?Met?Arg?Ala?Leu?Ser?Ser?Ser?Ala?Thr?Leu?Gln?Asp?Ser
180 185 190
Leu?Ala?Ile?Lys?Asp?Gly?Asp?Leu?Gly?Ser?Ser
195 200
<210>13
<211>203
<212>PRT
＜213〉domestic cat (Felis sivestris)
<400>13
Met?Ala?Leu?Leu?Leu?Pro?Leu?Leu?Thr?Ala?Leu?Ala?Leu?Leu?Thr?Cys
1 5 10 15
Arg?Pro?Gly?Gly?Ser?Leu?Gly?Cys?Ala?Leu?Pro?Gly?Ser?His?Ala?Gln
20 25 30
Val?Ser?Arg?Asp?Asn?Leu?Val?Leu?Leu?Gly?Gln?Met?Arg?Arg?Leu?Ser
35 40 45
Pro?Phe?Leu?Cys?Leu?Arg?Ala?Arg?Lys?Asp?Phe?Arg?Phe?Pro?Arg?Glu
50 55 60
Met?Leu?Glu?Gly?Gly?Gln?Leu?Arg?Glu?Ala?Gln?Ala?Ala?Ala?Thr?Val
65 70 75 80
Leu?Arg?Glu?Leu?Leu?Gln?Gln?Thr?Phe?Asn?Leu?Leu?His?Thr?Glu?Arg
85 90 95
Ser?Ser?Ala?Ala?Trp?Ser?Pro?Ala?Pro?Leu?His?Gly?Leu?Arg?Ser?Gly
100 105 110
Leu?His?Arg?Gln?Leu?Glu?Ala?Leu?Asp?Ala?Cys?Leu?Leu?Gln?Ala?Thr
115 120 125
Gly?Glu?Gly?Glu?Arg?Ala?Thr?Gly?Glu?Gly?Glu?Arg?Ala?Pro?Gly?Met
130 135 140
His?Gly?Pro?Val?Leu?Ala?Ile?Lys?Arg?Tyr?Phe?Gln?Asp?Ile?Arg?Val
145 150 155 160
Tyr?Leu?Glu?Asp?Glu?Gly?Tyr?Ser?Asp?Cys?Ala?Trp?Glu?Ile?Val?Arg
165 170 175
Leu?Glu?Ile?Met?Arg?Ala?Leu?Ser?Ser?Ser?Ala?Thr?Leu?Gln?Asp?Ser
180 185 190
Leu?Ala?Ile?Lys?Asp?Gly?Asp?Leu?Ala?Ser?Ser
195 200
<210>14
<211>591
<212>DNA
＜213〉domestic cat (Felis sivestris)
<400>14
atggccctcc?tgctccctct?gctgaccgcc?ctggcgctgc?tcacctgccg?ccctggaggc 60
tctctgggct?gtgccctgcc?tgggagccac?gcgcaggtta?gcagggacaa?cttggtgctc 120
ctgggccaga?tgcggagact?gtcccctttc?ttgtgcctgc?gggccagaaa?agacttccgc 180
ttcccccggg?agatgctgga?gggcggccag?ctccgggagg?cccaggccgc?cgccgccgtc 240
ctgcgggagc?tgctccagca?gaccttcaac?ctgttgcaca?cggagcgctc?ctcggcggcc 300
tggagccccg?cgccgctgca?cggactccgc?tctggcctcc?accggcagct?ggaagccctg 360
gacgcctgct?ggctgcaggc?cacgggcgag?ggagagcgcg?ccccggggat?gcacggccct 420
gccctggcca?tcaagaggta?cttccaggac?atccgcgtct?acctggagga?cgagggatac 480
agtgactgcg?cctgggaaat?tgtcaggctg?gaaatcatga?gagccttgtc?ctcctcggcg 540
accttgcaag?acagcttggc?catcaaggat?ggagacctgg?cgtcatcttg?a 591
<210>15
<211>591
<212>DNA
＜213〉domestic cat (Felis sivestris)
<400>15
atggccctcc?tgctccctct?actgaccgcc?ctggcgctgc?tcacctgccg?ccctggaggc 60
tctctgggct?gtgccctgcc?tgggagccac?gcgcaggtta?gcagggacaa?cttggtgctc 120
ctgggccaga?tgcggagact?gtcccctttc?ttgtgcctgc?gggccagaaa?agacttccgc 180
ttcccccggg?agatgctgga?gggcggccag?ctccgggagg?cccaggccgc?cgccgccgtc 240
ctgcgggagc?tgctccagca?gaccttcaac?ctgttgcaca?cggagcgctc?ctcggcggcc 300
tggagccccg?cgccgctgca?cggactccgc?tctggcctcc?accggcagct?ggaagccctg 360
gacgcctgct?tgctgcaggc?cacgggcgag?ggagagcgcg?ccccggggat?gcacggccct 420
gccctggcca?tcaagaggta?cttccaggac?atccgcgtct?acctggagga?cgagggatac 480
agtgactgcg?cctgggaaat?tgtcaggctg?gaaatcatga?gagccttgtc?ctcctcggcg 540
accttgcaag?acagcttggc?catcaaggat?ggagacctgg?cgtcatcttg?a 591
<210>16
<211>591
<212>DNA
＜213〉domestic cat (Felis sivestris)
<400>16
atggccctcc?tgctccctct?gctgaccgcc?ctggtgctgc?tcacctgccg?ccctggaggc 60
tctctgggct?gtgccctgcc?tgggagccac?gcgcaggtta?gcagggacaa?cttggtgctc 120
ctgggccaga?tgcggagact?gtcccctttc?ttgtgcctgc?gggccagaaa?agacttccgc 180
ttcccccggg?agatgctgga?gggcggccag?ctccgggagg?cccaggccgc?cgccgccgtc 240
ctgcgggagc?tgctccagca?gaccttcaac?ctgttgcaca?cggagcgctc?ctcggcggcc 300
tggagccccg?cgccgctgca?cggactccgc?tctggcctcc?accggcagct?ggaagccctg 360
gacgcctgct?tgctgcaggc?cacgggcgag?ggagagcgcg?ccccggggat?gcacggctct 420
gccctggcca?tcaagaggta?cttccaggac?atccgcgtct?acctggagga?cgagggatac 480
agtgactgcg?cctgggaaat?tgtcagactg?gaaatcatga?gagccttgtc?ctcctcggcg 540
accttgcaag?acagcttggc?catcaaggat?ggagacctgg?gctcatcttg?a 591
<210>17
<211>591
<212>DNA
＜213〉domestic cat (Felis sivestris)
<400>17
atggccctcc?tgctccctct?gctgaccgcc?ctggcgctgc?tcacctgccg?ccctggaggc 60
tctctgggct?gtgccctgcc?tgggagccac?gcgcaggtta?gcagggacaa?cttggtgctc 120
ctgggccaga?tgcggagact?gtcccctttc?ttgtgcctgc?gggccagaaa?agacttccgc 180
ttcccccggg?agatgctgga?gggcggccag?ctccgggagg?cccaggccgc?cgccgccgtc 240
ctgcgggagc?tgctccagca?gaccttcaac?ctgttgcaca?cggagcgctc?ctcggcggcc 300
tggagccccg?cgccgctgca?cggactccgc?tctggcctcc?accggcagct?ggaagccctg 360
gacgcctgct?ggctgcaggc?cacgggcgag?ggagagcgcg?ccccggggat?gcacggccct 420
gtcctggcca?tcaagaggta?cttccaggac?atccgcgtct?acctggagga?cgagggatac 480
agtgactgcg?cctgggaaat?tgtcaggctg?gaaatcatga?gagccttgtc?ctcctcggcg 540
accttacaag?acagcttggc?catcaaggat?ggagacctgg?gctcatcttg?a 591
<210>18
<211>591
<212>DNA
＜213〉domestic cat (Felis sivestris)
<400>18
atggccctcc?tgctccctct?gctgaccgcc?ctggcgctgc?tcacctgccg?ccctggaggc 60
tctctgggct?gtgccctgcc?tgggagccac?gcgcaggtta?gcagggacaa?cttggtgctc 120
ctgggccaga?tgcggagact?gtcccctttc?ttgtgcttgc?gggccagaaa?agacttccgc 180
ttcccccggg?agatgctgga?gggcggccag?ctccgggagg?cccaggccgc?cgccgccgtc 240
ctgcgggagc?tgctccagca?gaccttcaac?ctgttgcaca?cggagcgctc?ctcggcggcc 300
tggagccccg?cgccgctgca?cggactccgc?tctggcctcc?accggcagct?ggaagccctg 360
gacgcctgct?tgctgcaggc?cacgggcgag?ggagagcgcg?ccccggggat?gcacggccct 420
gccctggcca?tcaagaggta?cttccaggac?atccgcgtct?acctggagga?cgagggatac 480
agtgactgcg?cctgggaaat?tgtcaggctg?gaaatcatga?gagccttgtc?ctcctcggcg 540
accttgcaag?acagcttggc?catcaaggat?ggagacctgg?gctcatcttg?a 591
<210>19
<211>591
<212>DNA
＜213〉domestic cat (Felis sivestris)
<400>19
atggccctcc?tgctccctct?gctgaccgcc?ctggcgctgc?tcacctgccg?ccctggaggc 60
tctctgggct?gtgccctgcc?tgggagccac?gcgcaggtta?gcagggacaa?cttggtgctc 120
ctgggccaga?tgcggagact?gtcccctttc?ttgtgcctgc?gggccagaaa?agacttccgc 180
ttcccccggg?agatgctgga?gggcggccag?ctccgggagg?cccaggccgc?cgccgccgtc 240
ctgcgggagc?tgctccagca?gaccttcaac?ctgttgcaca?cggagcgctc?ctcggcggcc 300
tggagccccg?cgccgctgca?cggactccgc?tctggcctcc?accagcagct?ggaagccctg 360
gacgcctgct?tgctgcaggc?cacgggcgag?ggagagcgcg?ccctggggat?gcacggccct 420
gccctggcca?tcaagaggta?cttccaggac?atccgcgtct?acctggagga?cgagggatac 480
agtgactgcg?cctgggaaat?tgtcagactg?gaaatcatga?gagccttgtc?ctcctcggcg 540
accttgcaag?acagcttggc?catcaaggat?ggagacctgg?cgtcatcttg?a 591
<210>20
<211>591
<212>DNA
＜213〉domestic cat (Felis sivestris)
<400>20
atggccctcc?tgctccctct?gctgaccgcc?ctggcgctgc?tcacctgccg?ccctggaggc 60
tctctgggct?gtgccctgcc?tgggagccac?gcgcaggtta?gcagggacaa?cttggtgctc 120
ctgggccaga?tgcggagact?gtcccctttc?ttgtgcctgc?gggccagaaa?agacttccgc 180
ttcccccggg?agatgctgga?gggcggccag?ctccgggagg?cccaggccgc?cgccgccgtc 240
ctgcgggagc?tgctccagca?gaccttcaac?ctgttgcaca?cggagcgctc?ctcggcggcc 300
tggagccccg?cgccgctgca?cggactccgc?tctggcctcc?accggcagct?ggaagccctg 360
gacgcctgct?ggctgcaggc?cacgggcgag?ggagagcgcg?ccccggggat?gcacggccct 420
gtcctggcca?tcaagaggta?cttccaggac?atccgcgtct?acctggagga?cgagggatac 480
agtgactgcg?cctgggaaat?tgtcaggctg?gaaatcatga?gagccttgtc?ctcctcggcg 540
accttgcaag?acagcttggc?catcaaggat?ggagacctgg?cgtcatcttg?a 591
<210>21
<211>591
<212>DNA
＜213〉domestic cat (Felis sivestris)
<400>21
atggccctcc?tgctccctct?actgaccgcc?ctggcgctgc?tcacctgccg?ccctggaggc 60
tctctgggct?gtgccctgcc?tgggagccac?gcgcaggtta?gcagggacaa?cttggtgctc 120
ctgggccaga?tgcggagact?gtcccctttc?ttgtgcctgc?gggccagaaa?agacttccgc 180
ttcccccggg?agatgctgga?gggcggccag?ctccgggagg?cccaggccgc?cgccgccgtc 240
ctgcaggagc?tgctccagca?gaccttcaac?ctgttgcaca?cggagcgctc?ctcggcggcc 300
tggagccccg?tgccgctgca?cggactccgc?tctggcctcc?accggcagct?ggaagccctg 360
gacgcctgct?tgctgcaggc?cacgggcgag?ggagagcgcg?ccccggggat?gcacggccct 420
gccctggcca?tcaagaggta?cttccaggac?atccgcgtct?acctggagga?cgagggatac 480
agtgactgcg?cctgggaaat?tgtcagactg?gaaatcatga?gagccttgtc?ctcctcggcg 540
accttgcaag?acagcttggc?catcaaggat?ggagacctgg?cgtcatcttg?a 591
<210>22
<211>591
<212>DNA
＜213〉domestic cat (Felis sivestris)
<400>22
atggccctcc?tgctccctct?gctgaccgcc?ctggcgctgc?tcacctgccg?ccctggaggc 60
tctctgggct?gtgccctgcc?tgggagccac?gcgcaggtta?gcagggacaa?cttggtgctc 120
ctgggccaga?tgcggagact?gtcccctttc?ttgtgcctgc?gggccagaaa?agacttccgc 180
ttcccccggg?agatgctgga?gggcggccag?ctctgggagg?cccaggccgc?cgccgccgtc 240
ctgcgggagc?tgctccagca?gaccttcaac?ctgttgcaca?cggagcgctc?ctcggcggcc 300
tggagccccg?cgccgctgca?cggactccgc?tctggcctcc?accggcagct?ggaagccctg 360
gacgcctgct?tgctgcaggc?cacgggcgag?ggagagcgcg?ccccggggat?gcacggccct 420
gccctggcca?tcaagaggta?cttccaggac?atccgcgtct?acctggagga?cgagggatac 480
agtgactgcg?cctgggaaat?tgtcaggctg?gaaatcatga?gagccttgtc?ctcctcggcg 540
accttgcaag?acagcttggc?catcaaggat?gaagacctgg?gctcatcttg?a 591
<210>23
<211>591
<212>DNA
＜213〉domestic cat (Felis sivestris)
<400>23
atggccctcc?tgctccctct?gctgaccgcc?ctggcgctgc?tcacctgccg?ccctggaggc 60
tctctgggct?gtgccctgcc?tgggagccac?gcgcaggtta?gcagggacaa?cttggtgctc 120
ctgggccaga?tgcggagact?gtcccctttc?ttgtgcctgc?gggccagaaa?agacttccgc 180
ttcccccggg?agatgctgga?gggcggccag?ctccgggagg?cccaggccgc?cgccgccgtc 240
ctgcgggagc?tgctccagca?gaccttcaac?ctgttgcaca?cggagcgctc?ctcggcggcc 300
tggagccccg?cgccgctgca?cggactccgc?tctggcctcc?accggcagct?ggaagccctg 360
gacgcctgct?tgctgcaggc?cacgggcgag?ggagagcgcg?ccccggggat?gcacggccct 420
gccctggcca?tcaagaggta?cttccaggac?atccgcgtct?acctggagga?cgagggatac 480
agtgactgcg?cctgggaaat?tgtcaggctg?gaaatcatga?gagccttgtc?ctcctcggcg 540
accttgcaag?acagcttggc?catcaaggat?ggagacctgg?gcttatcttg?a 591
<210>24
<211>591
<212>DNA
＜213〉domestic cat (Felis sivestris)
<400>24
atggccctcc?tgctccctct?gctgaccgcc?ctggcgctgc?tcacctgccg?ccctggaggc 60
tctctgggct?gtgccctgcc?cgggagccac?acgcaggtta?gcagggacaa?cttggtgctc 120
ctgggccaga?tgcggagact?gtcccctttc?ttgtgcctgc?gggccagaaa?agacttccgc 180
ttcccccggg?agatgctgga?gggcggccag?ctccgggagg?cccaggccgc?cgccgccgtc 240
ctgcgggagc?tgctccagca?gaccttcaac?ctgttgcaca?cggagcgctc?ctcggcggcc 300
tggggccccg?cgccgctgca?cggactccgc?tctggcctcc?accggcagct?ggaagccctg 360
gacgcctgct?tgctgcaggc?cacgggcgag?ggagagcgcg?ccccggggat?gcacggccct 420
gacctggcca?tcaagaggta?cttccaggac?atccgcgtct?acctggagga?cgagggatac 480
agtgactgcg?cctgggaaat?tgtcaggctg?gaaatcatga?gagccttgtc?ctcctcggcg 540
accttgcaag?acagcttggc?catcaaggat?ggagacctgg?gctcatcttg?a 591
<210>25
<211>612
<212>DNA
＜213〉domestic cat (Felis sivestris)
<400>25
atggccctcc?tgctccctct?actgaccgcc?ctggcgctgc?tcacctgccg?ccctggaggc 60
tctctgggct?gtgccctgcc?tgggagccac?gcgcaggtta?gcagggacaa?cttggtgctc 120
ctgggccaga?tgcggagact?gtcccctttc?ttgtgcctgc?gggccagaaa?agacttccgc 180
ttcccccggg?agatgctgga?gggcggccag?ctccgggagg?cccaggccgc?cgccgccgtc 240
ctgcgggagc?tgctccagca?gaccttcaac?ctgttgcaca?cggagcgctc?ctcggcggcc 300
tggagccccg?cgccgctgca?cggactccgc?tctggcctcc?accggcagct?ggaagccctg 360
gacgcctgct?tgctgcaggc?cacgggcgag?ggagagcgcg?ccacgggcga?gggagagcgc 420
gccccgggga?tgcacggccc?tgtcctggcc?atcaagaggt?acttccagga?catccgcgtc 480
tacctggagg?acgagggata?cagtgactgc?gcctgggaaa?ttgtcaggct?ggaaatcatg 540
agagccttgt?cctcctcggc?gaccttgcaa?gacagcttgg?ccatcaagga?tggagacctg 600
ggctcatctt?ga 612
<210>26
<211>612
<212>DNA
＜213〉domestic cat (Felis sivestris)
<400>26
atggccctcc?tgctccctct?gctgaccgcc?ctggcgctgc?tcacctgccg?ccctggaggc 60
tctctgggct?gtgccctgcc?tgggagccac?gcgcaggtta?gcagggacaa?cttggtgctc 120
ctgggccaga?tgcggagact?gtcccctttc?ttgtgcctgc?gggccagaaa?agacttccgc 180
ttcccccggg?agatgctgga?gggcggccag?ctccgggagg?cccaggccgc?cgccaccgtc 240
ctgcgggagc?tgctccagca?gaccttcaac?ctgttgcaca?cggagcgctc?ctcggcggcc 300
tggagccccg?cgccgctgca?cggactccgc?tctggcctcc?accggcagct?ggaagccctg 360
gacgcctgct?tgctgcaggc?cacgggcgag?ggagagcgcg?ccacgggcga?gggagagcgc 420
gccccgggga?tgcacggccc?tgtcctggcc?atcaagaggt?acttccagga?catccgcgtc 480
tacctggagg?acgagggata?cagtgactgc?gcctgggaaa?ttgtcaggct?ggaaatcatg 540
agagccttgt?cctcctcggc?gaccttgcaa?gacagcttgg?ccatcaagga?tggagacctg 600
gcgtcatctt?ga 612
Claims (12)
1, cat omega interferon is the protein with one of amino acid residue sequence of following cat omega interferon family:
1) the SEQ ID № in the sequence table: 1;
2) the SEQ ID № in the sequence table: 2;
3) the SEQ ID № in the sequence table: 3;
4) the SEQ ID № in the sequence table: 4;
5) the SEQ ID № in the sequence table: 5;
6) the SEQ ID № in the sequence table: 6;
7) the SEQ ID № in the sequence table: 7;
8) the SEQ ID № in the sequence table: 8;
9) the SEQ ID № in the sequence table: 9;
10) the SEQ ID № in the sequence table: 10;
11) the SEQ ID № in the sequence table: 11;
12) the SEQ ID № in the sequence table: 12;
13) the SEQ ID № in the sequence table: 13;
14) with SEQ ID № in the sequence table: the amino acid residue sequence of 1-13 is through replacement, disappearance or the interpolation of one to ten amino-acid residue and feline leukaemia virus, FIV and canine parvovius are had the protein of anti-infectious function.
2, the gene of coding claim 1 described cat omega interferon is selected from one of nucleotide sequence of following cat omega interferon family:
1) SEQ ID № in the sequence table: 14 dna sequence dna;
2) SEQ ID № in the sequence table: 15 dna sequence dna;
3) SEQ ID № in the sequence table: 16 dna sequence dna;
4) SEQ ID № in the sequence table: 17 dna sequence dna;
5) SEQ ID № in the sequence table: 18 dna sequence dna;
6) SEQ ID № in the sequence table: 19 dna sequence dna;
7) SEQ ID № in the sequence table: 20 dna sequence dna;
8) SEQ ID № in the sequence table: 21 dna sequence dna;
9) SEQ ID № in the sequence table: 22 dna sequence dna;
10) SEQ ID № in the sequence table: 23 dna sequence dna;
11) SEQ ID № in the sequence table: 24 dna sequence dna;
12) SEQ ID № in the sequence table: 25 dna sequence dna;
13) SEQ ID № in the sequence table: 26 dna sequence dna;
17) nucleotide sequence of the dna sequence dna hybridization that under the rigorous condition of height, can limit with SEQ ID №: 14-26 in the sequence table;
18) SEQ ID № in the code sequence tabulation: 1 proteic dna sequence dna;
19) SEQ ID № in the code sequence tabulation: 2 proteic dna sequence dnas;
20) SEQ ID № in the code sequence tabulation: 3 proteic dna sequence dnas;
21) SEQ ID № in the code sequence tabulation: 4 proteic dna sequence dnas;
22) SEQ ID № in the code sequence tabulation: 5 proteic dna sequence dnas;
23) SEQ ID № in the code sequence tabulation: 6 proteic dna sequence dnas;
24) SEQ ID № in the code sequence tabulation: 7 proteic dna sequence dnas;
25) SEQ ID № in the code sequence tabulation: 8 proteic dna sequence dnas;
26) SEQ ID № in the code sequence tabulation: 9 proteic dna sequence dnas;
27) SEQ ID № in the code sequence tabulation: 10 proteic dna sequence dnas;
28) SEQ ID № in the code sequence tabulation: 11 proteic dna sequence dnas;
29) SEQ ID № in the code sequence tabulation: 12 proteic dna sequence dnas;
30) SEQ ID № in the code sequence tabulation: 13 proteic dna sequence dnas.
3, the expression vector that contains the described cat omega interferon encoding gene of claim 2.
4, the transgenic cell line that contains the described cat omega interferon encoding gene of claim 2.
5, the host bacterium that contains the described expression vector of claim 3.
6, a kind of method of expressing the described cat omega interferon of claim 1 is that the recombinant expression vector that will contain the described cat omega interferon encoding gene of claim 2 imports host cell, expresses obtaining cat omega interferon.
7, according to the method for the described cat omega interferon of claim 6, it is characterized in that: the described carrier that sets out that is used to make up the recombinant expression vector that contains the described cat omega interferon encoding gene of claim 2 is pLLp-STII, pPICZ α A, pET carrier series, pQE carrier series, pPIC9K or pPICZ α.
8, according to the method for the described cat omega interferon of claim 7, it is characterized in that: described recombinant expression vector is FeIFN-ω/pLLp-STII.
9, according to the method for the described cat omega interferon of claim 7, it is characterized in that: described recombinant expression vector is FeIFN-ω/pPICZ α A.
10, according to claim 6 or 7 or 8 or 9 described methods, it is characterized in that: described host is E.coliDH5 α, E.coli TB1, E.coli DE3, yeast SMD1168H, yeast GS115, yeast X-33 or yeast KM71H.
11, according to claim 6 or 7 or 8 or 9 described methods, it is characterized in that: described host needs to add IPTG and carry out abduction delivering when the colibacillus, add IPTG concentration be 0.2-1mM; When described host is yeast, need adds methyl alcohol and carry out abduction delivering, add methyl alcohol final concentration be 0.1-1.0%.
12, the application of the described cat omega interferon of claim 1 in the medicine that the anti-feline leukaemia virus of preparation, FIV or canine parvovius infect.
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| CN1919870B (en) * | 2006-09-13 | 2010-11-24 | 中国科学院微生物研究所 | Cat alpha interferon, encode gene and application thereof |
| CN108276486B (en) * | 2015-04-23 | 2021-06-15 | 中国农业科学院生物技术研究所 | Cat omega 2 interferon mutant and preparation method and application thereof |
| CN109206501B (en) * | 2018-09-26 | 2022-03-04 | 东北农业大学 | Cat interferon omega, coding gene thereof and application of cat interferon omega in antiviral aspect |
| CN109206502B (en) * | 2018-09-26 | 2022-03-04 | 东北农业大学 | Cat interferon omega, preparation method thereof and application thereof in antivirus |
| CN112695053B (en) * | 2021-02-05 | 2022-03-29 | 华中农业大学 | Preparation method of recombinant cat interferon |
| CN113121672B (en) * | 2021-04-20 | 2023-01-03 | 甘肃农业大学 | Soluble prokaryotic expression and purification method of cat interferon gamma and application |
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| JP2001151692A (en) * | 1999-09-09 | 2001-06-05 | Toray Ind Inc | Method and agent for treating disease infected with canine parvovirus |
| US6350443B1 (en) * | 1997-10-30 | 2002-02-26 | Toray Industries, Inc. | Method of treatment for feline leukemia virus infections |
| CN1365983A (en) * | 2001-01-19 | 2002-08-28 | 重庆富进生物医药有限公司 | Human alpha interferon derivative with superstrong antiviral activity |
| CN1444993A (en) * | 2003-04-25 | 2003-10-01 | 中国人民解放军军事医学科学院微生物流行病研究所 | Application of the recombination human interferon omega in preparing medicine for preventing and treating not typical model pneumonia |
| CN1539971A (en) * | 2003-04-25 | 2004-10-27 | 中国人民解放军军事医学科学院微生物 | Method for preparing human interferon omega by using means of gene engineering |
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| US6350443B1 (en) * | 1997-10-30 | 2002-02-26 | Toray Industries, Inc. | Method of treatment for feline leukemia virus infections |
| JP2001151692A (en) * | 1999-09-09 | 2001-06-05 | Toray Ind Inc | Method and agent for treating disease infected with canine parvovirus |
| CN1365983A (en) * | 2001-01-19 | 2002-08-28 | 重庆富进生物医药有限公司 | Human alpha interferon derivative with superstrong antiviral activity |
| CN1444993A (en) * | 2003-04-25 | 2003-10-01 | 中国人民解放军军事医学科学院微生物流行病研究所 | Application of the recombination human interferon omega in preparing medicine for preventing and treating not typical model pneumonia |
| CN1539971A (en) * | 2003-04-25 | 2004-10-27 | 中国人民解放军军事医学科学院微生物 | Method for preparing human interferon omega by using means of gene engineering |
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Assignee: Beijing HFK Bioscience Co., Ltd. Assignor: Institute of Microbiology, Chinese Academy of Sciences Contract record no.: 2010110000163 Denomination of invention: Cat omega interferon and its coding gene and uses Granted publication date: 20070502 License type: Exclusive License Open date: 20060208 Record date: 20100928 |