CN1307889C - Edible oil possessing efficacy of lowering down blood fat - Google Patents
Edible oil possessing efficacy of lowering down blood fat Download PDFInfo
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- CN1307889C CN1307889C CNB031029833A CN03102983A CN1307889C CN 1307889 C CN1307889 C CN 1307889C CN B031029833 A CNB031029833 A CN B031029833A CN 03102983 A CN03102983 A CN 03102983A CN 1307889 C CN1307889 C CN 1307889C
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Abstract
The present invention relates to edible oil with the efficacy of lowering blood fat, which is at least proportional edible oil prepared by mixing first vegetable oil and second vegetable oil, wherein contained unsaturated fatty acid accounts for more than 85 wt%, multiple unsaturated fatty acid accounts for about 26 to 32 wt%, and unit unsaturated fatty acid accounts for 59 to 69 wt%.
Description
Technical field
The present invention relates to a kind of prescription of edible oil, and particularly relate to a kind of edible oil that reduces the blood fat effect that has.
Background technology
The present grease picked-up kenel that studies show that has very big correlation with the probability of suffering from angiocardiopathy.Wherein, (the Saturated Fatty Acids of the saturated fatty acid in the grease; SFAs) for improving triglycerides (Triglyceride in the blood; TG) with the arch-criminal of cholesterol (cholesterol) concentration, and the closely bound up (Am.J.Clin.Nutr.1997 of triglyceride and cholesterol and angiocardiopathy; 66 (suppl): 988S-990S; Am.J.Cardiol.1998; 81 (4A), 18B-25B; Am.J.Cardiol.1998; 82,22Q-25Q; Nutr.56:895-898,1992).
So the eating habit of the kenel of the high grease picked-up of compatriots' preference American-European countries in recent years, and then cause the probability of suffering from cardiovascular disease to improve, the summation of the present cerebrovascular and cardiovascular disease is second of the Taiwan ten big causes of death.Find relatively that according to the Taiwan nutrition survey that Taiwan Department of Health announces the ratio that the fat that compatriots absorbed accounts for total amount of heat rises year by year.With Taiwan 20-34 year man is example: Christian era, fat accounted for 31.7% of total picked-up heat during 1980-1981, and the ratio that 1993-1996 fat accounts for total picked-up heat rises to 34.6%.The saturated fatty acid ratio of absorbing in addition in the fat also rises to 33% of total fatty acid content by 25%.In addition, platform large hospital nucleon medical board research report find from 1981 to calendar year 2001, the high person of compatriots' blood cholesterol concentration is increased to 12.7% from 4.3%, and the high person of triglyceride concentration is increased to 24.1% from 6.5% in state's human blood.Again according to (the American Heart Association of American Heart Association; AHA) show in investigation statistics in 1999 that American's fat of on average ingesting accounts for more than 34% of total picked-up heat, the saturated fatty acid ratio of wherein absorbing in the fat is about 42%.
Because angiocardiopathy is first of the U.S.'s ten big causes of the death, therefore AHA advises that total fatty intake should be no more than every day and absorb 30% of total amount of heat, the saturated fatty acid intake should be not more than and absorb 8% of total amount of heat every day, polybasic unsaturated fatty acid (Poly-Unsaturated Fatty Acids; PUFAs) intake should be not more than and absorb 7% of total amount of heat every day, and monounsaturated fatty acid (Mono-UnsaturatedFatty Acids; MUFAs) intake should be not less than and absorb 15% of total amount of heat every day.NCEP (National cholesterol education program) advises that also total fatty intake should be no more than every day and absorb 30% of total amount of heat in addition, wherein the phase I should be controlled at saturated fatty acid and absorb total amount of heat 8-10% every day, and the polybasic unsaturated fatty acid intake should be not more than and absorb 10% of total amount of heat every day, and the monounsaturated fatty acid intake should be not less than and absorb 15% of total amount of heat every day.Second stage is then reduced to the saturated fatty acid intake and is absorbed below 7% of total amount of heat every day.So will make monounsaturated fatty acid is main oil source, for reducing blood fat positive effect will be arranged.Its main cause except aforesaid saturated fatty acid can increase triglyceride in the blood and cholesterol, polybasic unsaturated fatty acid has plurality purpose carbon-carbon double bond because of it, thereby oxidized easily than monounsaturated fatty acid.Therefore do not advise absorbing too many saturated fatty acid and polybasic unsaturated fatty acid, and be main grease picked-up source with monounsaturated fatty acid.
Yet grease is the indispensable nutritional labeling that earns a bare living, except origin of heat is provided, simultaneously also for forming the important substance of membrane structure.Metabolism is synthetic in vivo will produce different physiological reactions for studies show that in recent years, various aliphatic acid, thereby has remarkable influence for health.For example (C18: 2) (C18: 3), be the material that can't synthesize in the human body, degradation ill symptoms under easily cause during shortage growing retardation, skin injury, neurogenic disease and the eyesight must aliphatic acid so belong to linolenic acid for linolenic acid.Linolenic acid and linolenic acid, except the higher oxidized easily risk of degree of unsaturation, ratio between the two also is quite important.
Linolenic acid becomes arachidonic acid (C20: 4) via internal metabolism, linolenic acid then is metabolized to EPA or DHA, these metabolites are oxidized to prostaglandin (prostaglandins) or leukotriene (leukotrienes) via cyclo-oxygenase (cyclooxgenase) and lipoxidase (lipoxygenase) again, these two kinds of materials are that normal physiological is required, but also can bring relevant disease such as angiocardiopathy, inflammatory response etc.Usually prostaglandin that is come by arachidonic acid metabolic or leukotriene have the character of the inflammation of inspiring, and prostaglandin or leukotriene that EPA or DHA metabolism come then have the effect of anti-inflammatory.It is essential to take into account to satisfy life institute with the time at both need, and produces under the situation of less inflammation material, and the ratio between linolenic acid and the linolenic acid is then quite important.At present existing scholar points out, the ratio of linolenic acid and linolenic acid is an optimal proportion at 1-4 in the grease, and two kinds of essential fatty acids not only are provided, and can produce more DHA under this kind ratio, growth and angiocardiopathy to brain all have positive effect (J.Nutr, 1998; Biochem Biophys Acta, 1992; Artemis P.Simopoulos workshop on EFA, NIH, Maryland USA, 1999; DOH, 1996; J.Lipid Research 2002; 43,1537-1543).
Summary of the invention
Purpose of the present invention is exactly that a kind of edible oil that reduces the blood fat effect that has is being provided, and the best picked-up ratio that meets linolenic acid/linolenic acid with the grease picked-up standard that meets general recommendations again is the suggestion between 1-4.
According to above-mentioned and other purpose of the present invention, a kind of edible oil that reduces the blood fat effect that has is proposed.This edible oil is the blending edible oil that is mixed by first vegetable oil and second vegetable oil at least, and wherein contained unrighted acid is at least about 85 percentage by weights.Above-mentioned first vegetable oil comprises olive oil, and second vegetable oil comprises the mustard caul-fat.
According to above-mentioned and other purpose of the present invention, a kind of edible oil that reduces the blood fat effect that has is proposed.This edible oil is the blending edible oil that is mixed by first vegetable oil and second vegetable oil at least, and the ratio of wherein contained saturated fatty acid, polybasic unsaturated fatty acid and monounsaturated fatty acid is about 2-3: 8-9: 18-20.According to a preferred embodiment of the present invention, the linolenic acid in the wherein above-mentioned polybasic unsaturated fatty acid and the ratio of linolenic acid are about 2-3.Above-mentioned first vegetable oil comprises olive oil, and second vegetable oil comprises the mustard caul-fat.
According to above-mentioned and other purpose of the present invention, a kind of edible oil that reduces the blood fat effect that has is proposed.This edible oil is the blending edible oil that is mixed by first vegetable oil and second vegetable oil at least, and it contains the polybasic unsaturated fatty acid of the saturated fatty acid of the 6-10 percentage by weight of having an appointment, about 26-32 percentage by weight and the monounsaturated fatty acid of about 59-69 percentage by weight.Above-mentioned first vegetable oil comprises olive oil, and second vegetable oil comprises the mustard caul-fat.
According to above-mentioned and other purpose of the present invention, a kind of edible oil that reduces the blood fat effect that has is proposed.This edible oil is the blending edible oil that is mixed by first vegetable oil and second vegetable oil at least, and it contains the linolenic acid (18: 2) of the oleic acid (18: 1) of the 58-68 percentage by weight of having an appointment, about 19-24 percentage by weight and the linolenic acid (18: 3) of about 6-9 percentage by weight.Above-mentioned first vegetable oil comprises olive oil, and second vegetable oil comprises the mustard caul-fat.
From the above, blending edible oil provided by the present invention, the grease that not only meets general recommendations absorbs standard, and the best picked-up ratio that meets linolenic acid/linolenic acid again is for the suggestion between 1-4, so have the effect that downgrades blood fat.
The specific embodiment
In order to concoct out the grease picked-up standard that not only meets general recommendations but also the best picked-up ratio that meets linolenic acid/linolenic acid to be the suggestion between 1-4, therefore the invention provides a kind of edible oil that reduces the blood fat effect that has.
The mediation of edible oil
According to the meals suggestion of AHA and NCEP, the essential fatty acid that polybasic unsaturated fatty acid provides human body not synthesize on the one hand is on the one hand because the degree of unsaturation height is easier to oxidation, so the control polybasic unsaturated fatty acid then can reduce the chance of oxidation.Monounsaturated fatty acid then has the effect that reduces cholesterol, so monounsaturated fatty acid concentration is improved, angiocardiopathy is had positive influence.
The present invention accounts for the suggestion calculating of total amount of heat 30% with the grease daily intake of AHA and NCEP, with saturated fatty acid, polybasic unsaturated fatty acid and monounsaturated fatty acid ratio, utilize the different vegetable oil of mediation, it is adjusted into 2-3: 8-9: 18-20 makes its grease that meets general recommendations picked-up standard.The saturated fatty acid ratio that this fatty acid proportion not only will have the angiocardiopathy negative effect reduces, and increases the monounsaturated fatty acid that angiocardiopathy is had positive influences, to increase the effect of its reducing blood lipid.In addition, the present invention also is adjusted at the ratio of linolenic acid/linolenic acid in the grease between the 2-3, makes the present invention have more positive influence to angiocardiopathy.
According to a preferred embodiment of the present invention, the olive oil (Olive Oil) of 12 percentage by weights and mustard caul-fat (Canola Oil) mediation of 88 percentage by weights are formed the percentage by weight check data such as the table one of the content of fatty acid of each batch blending edible oil (calling beneficial heavy wool in the following text).Because the place of production is different with season, though be both olive oil and mustard caul-fat, its content of fatty acid can change to some extent, can get a scope with the conservative estimation of 5% error.From table one as can be known, the beneficial heavy wool that this preferred embodiment mixed, its saturated fatty acid (SFAs) content is about the 6-10 percentage by weight, monounsaturated fatty acid (MUFAs) content is about the 59-69 percentage by weight, polybasic unsaturated fatty acid (PUFAs) content is about the 26-32 percentage by weight, so its unrighted acid (UFAs) content is about more than 85 percentage by weights.
Table one: the percentage by weight check data of the content of fatty acid of the beneficial heavy wool of each batch.
| Aliphatic acid (percentage by weight) | Mission Number | |||||
| 1 | 2 | 3 | 4 | 5 | 6 | |
| 16∶0 18∶0 16∶1 18∶1 18∶2 18∶3 18∶2/18∶3 | 5.14 2.12 0.22 63.14 21.24 7.82 2.72 | 5.13 2.13 0.26 63.1 21.24 7.89 2.69 | 5.25 1.52 0.43 63.76 21.79 7.25 3.00 | 5.13 2.18 0.45 62.24 21.9 7.87 2.78 | 5.51 2.13 0.71 62.26 21.13 7.89 2.68 | 5.45 2.28 0.24 62.82 21.25 7.69 2.76 |
| SFAs MUFAs PUFAs UFAs S∶P∶M(30％energy) | 6-10 59-69 26-32 85 above 2-3: 8-9: 18-20 | |||||
Experiment one: compare with edible oil with high level saturated fatty acid
Experiment one is to test about downgrading the blood fat function appraisal procedure with reference to the healthy food that Taiwan Department of Health of Executive Yuan announces, mainly the zootype with hamster and every group of each 8 feeding high cholesterol diet of C3H/HeJ mouse carries out in vivo studies.Experiment one is eight weeks by a definite date, in the blood sampling of 2,4,8 weeks, analyze the concentration of cholesterol in its blood plasma (TC), LDL-C (LDL-C) and triglycerides (TG) respectively, and note down the body weight change and the average intake of feed every day of every animal.The main purpose of experiment one is to verify animal when absorbing the high cholesterol diet, compares with the ready-mixed oil that beneficial heavy wool comes and saturated fatty acid content is higher that preferred embodiment of the present invention was provided, and whether beneficial heavy wool can reach the function that downgrades blood fat.The preparation of animal feed:
The preparation of animal feed mainly is that (AmericanInstitute of Nutrition:Report of American Institute of NutritionAd Hoc Committee on Standards for Nutritional Studies.J.Nutr.1977 107:1340-1344.) is the basal feed prescription with not grease-contained AIN 76A purifying daily ration.Control group and experimental group include 0.5% cholesterol according to the required preparation respectively of experiment and the grease heat is the high grease feed formula of feed total amount of heat 13%.Table two is formed table for feed formula, in respect of casein (Casein), sucrose (sucrose), choline (choline), methionine (methionine), vitamin, mineral matter, cellulose, cornstarch (corn starch), cholesterol, ready-mixed oil and beneficial heavy wool, wherein ready-mixed oil is the grease of the higher unsaturated fatty acid concentration of vegetable oil and animal oil mediation.As shown in Table 3, control group includes the saturated fatty acid of 51.1% monounsaturated fatty acid, 20.1% polybasic unsaturated fatty acid and 28.8%, and experimental group includes the saturated fatty acid of 63.7% monounsaturated fatty acid, 29.1% polybasic unsaturated fatty acid and 7.3%.The solid shapeization of feed is then finished with two-axis extruder.
Table two: feed formula is formed
| Composition | Control group (percentage by weight) | Experimental group (percentage by weight) |
| Cornstarch casein sucrose ready-mixed oil benefit heavy wool cellulose mineral matter Vitamin A methyllanthionine choline cholesterol | 44 20 20 6 - 5 3.5 1 0.3 0.2 0.5 | 44 20 20 - 6 5 3.5 1 0.3 0.2 0.5 |
Table three: the aliphatic acid of feed formula grease is formed
| Aliphatic acid | Ready-mixed oil (%) | Benefit heavy wool (%) |
| 16∶0 16∶1 18∶0 18∶1 | 21.01 2.24 7.50 48.90 | 5.14 0.22 2.12 63.14 |
| 18∶2 18∶3 C18∶2/C18∶3 | 15.89 4.17 3.81 | 21.24 7.82 2.72 |
| SFAs MUFAs PUFAs UFAs S∶P∶M(30％energy) | 28.80 51.14 20.06 71.20 8.6∶6.0∶15.3 | 7.26 63.68 29.06 92.74 2.2∶8.7∶19.1 |
Analytical method is according to Official Methods and Recommended Practices of the AOCSCe1-62.
Animal varieties and feeding and management:
The male Syria golden hamster (Syrian golden hamster) in six ages in week is available from medical college of Taiwan Univ. Experimental Animal Center, the SPF grade six ages in week male C3H/HeJ mouse available from Taiwan National Science Council country Experimental Animal Center.
Hamster and C3H/HeJ mouse all adopt the ear tag mode, as the foundation of daily management record in the experimentation.Hamster and C3H/HeJ mouse are all raised in the raising in the box of PC (polycarbonate) material, and every day, required feed all picked up from by ingesting with drinking-water, and in regularly replace weekly bedding and padding (BetaChip, U.S.A.) and water bottle.Main raising condition is: 23 ± 2 ℃ of temperature, 60 ± 10%, 12 hours illumination/dark of relative humidity are alternately.Also write down the weight of animals and feed intake weekly, experimental session also is responsible for the observation of the daily health of animal used as test by the veterinarian.
Before the experiment beginning, the male hamster and the C3H/HeJ mouse in six ages in week need earlier with Purina 5001 diet (LabDiet
, 5001 Rodent diet, Brentwood, MO, USA) the health observation is carried out in raising.Behind the Yu Erzhou, formally carry out the blood fat that downgrades of the beneficial heavy wool that preferred embodiment of the present invention provided and test.With two groups of hamsters (8 every group) and two groups of C3H/HeJ mouses (24 every group), raise with following feed condition respectively:
1. control group: include 0.5% cholesterol and grease calorie intake and account for feed total amount of heat (grossenergy, GE) 13% ready-mixed oil feed, wherein monounsaturated fatty acid, polybasic unsaturated fatty acid and saturated fatty acid respectively are 51.1%, 20.1% and 28.8%.
2. experimental group: include 0.5% cholesterol and grease calorie intake and account for feed total amount of heat (grossenergy, GE) 13% beneficial heavy wool feed, wherein monounsaturated fatty acid, polybasic unsaturated fatty acid and saturated fatty acid respectively are 63.7%, 29.1% and 7.3%.
Sample is collected and is analyzed:
Hamster, C3H/HeJ mouse experiment schedule to last totally eight weeks, carry out blood sampling in 2,4 and 8 weeks.Hamster and C3H/HeJ mouse need about 12 hours of fasting in advance before each blood sampling, hamster with the gas anesthesia machine (MDS matrx, U.S.A.) carry out shallow-layer anesthesia after, directly collect blood sample with the heart blood sampling.C3H/HeJ mouse with carbon dioxide euthanasia after, directly heart blood sampling.Each is gathered blood places the centrifuge tube that does not contain anticoagulant, and in 4 ℃ leave standstill 5-6 hour after, with 360 * g centrifugal 30 minutes, collect serum, again it is stored in low temperature-80 ℃, carry out lipid determination to treat the next day.
The blood fat analysis comprises that TC, TG, HDL-C and LDL-C utilize the serum biochemistry instrument (KonnelabCo. Finland) measures.Wherein the required standard items of fatty acid compositional analysis available from SigmaChemical Co. (St Louis, MO, USA); Comprise T-CHOL (Total Cholesterol, TC), triglycerides (Triglyceride; TG), HDL-C (High DensityLipoprotein-Cholesterol; HDL-C), LDL-C (Low DensityLipoprotein-Cholesterol; LDL-C) the required reagent of lipid determination is available from Konnelab Co. (Finland); Normal saline solution is available from the biochemical pharmacy of Ji.
Table four: the variation of hamster feeding different experiments feed two, four, eight all each blood fat biochemical values.
| Suppress percentage * | -12.0％ b | -23.8％ b | -23.6％ b |
| TG | mg/dL | ||
| The control group experimental group suppresses percentage | 513.0±89.9 419.1±101.0 -18.3％ b | 452.6±129.6 322.7±93.0 -28.7％ b | 467.7±146.4 313.8±41.6 -27.9％ b |
* (experimental group-control group)/control group * 100%.
aNumerical value is significantly different (p＜0.1).
bNumerical value is significantly different (p＜0.05).
cNumerical value is significantly different (p=0.103) not.
Experimental data adopts SPSS (Statistical Package for the Social Science) statistical analysis software to carry out variance (ANOVA) analysis, and with Duncan ' s Test relatively between each factor the significant difference degree (p＜0.05=, the gained data are represented with mean value ± standard deviation (n=8).
Table five: the variation of C3H/HeJ mouse feeding different experiments feed two, four, eight all each blood fat biochemical values
* (experimental group-control group)/control group * 100%
bNumerical value is significantly different (p＜0.05).
Experimental data adopts SPSS (Statistical Package for the Social Science) statistical analysis software to carry out variance (ANOVA) analysis, and with significant difference degree (p＜0.05) between each factor of Duncan ' s Test comparison, the gained data are represented with mean value ± standard deviation (n=24).
Experimental result:
As shown in Table 4, in the high cholesterol meals, as with beneficial heavy wool that preferred embodiment of the present invention was provided oil source, make at 2,4,8 Zhou Houke that TC, LDL-C and TG content significantly reduce in the hamster blood as the feeding hamster.As shown in Table 5, in the high cholesterol meals, as with beneficial heavy wool that preferred embodiment of the present invention the was provided oil source as the feeding C3H/HeJ mouse, after 2,4,8 weeks, TC, LDL-C and TG content also have remarkable reduction in its blood.From the above, the result of experiment one proves that beneficial heavy wool can downgrade the blood fat that reduces in the feeding high cholesterol animal blood serum really, comprises TC, LDL-C and TG.
Experiment two: compare with edible oil with lower content saturated fatty acid
Experiment two is tested with reference to the relevant appraisal procedure that downgrades blood fat function in the healthy food efficacy assessment of Taiwan Department of Health of Executive Yuan announcement, selection is an experimental animal models with hamster (Syrian hamsters), inquires into the influence of the beneficial heavy wool of preferred embodiment of the present invention to the live body Blood Lipid.Whether its purpose if the lower oil product of the beneficial heavy wool of preferred embodiment of the present invention and saturated fatty acid content is compared, still has the effect that downgrades blood fat for inquiring into.
Animal varieties and raising condition:
Animal used as test is 8 all male hamsters in age (Syrian hamsters), available from Taiwan National Science Council animal center.The hamster of buying is raised after a period of time conforms it with commercial formulation feed (chow diet) earlier, and animal is raised respectively in stainless steel suspention cage in mode of a cage.Animal House is kept humidity 60% ± 10%, and room temperature is controlled at 22-25 ℃, and reaching with autotimer control light application time every day is 6:00 AM each bright dark circulation in 14,10 hours at 8 in evening.Water and feed all pick up from by picked-up, and routine observation also writes down the feed intake of hamster, and the scale body weight once weekly.
This experiment is divided into 2 groups at random with the hamster of buying, 10 every group.Animal is raised respectively and gives control or the feed of different greases totally 6 weeks, with hamster fasting 12-16 hour, sacrifices and gathers its blood, and carry out every analysis after the experiment expiration.
Feed ingredient and source:
The detailed composition of feed please see Table six, in respect of casein (casein), cholesterol (cholesterol), gelatinization cornstarch (dextrinized cornstarch), cellulose (alphacel), AIN-93 vitamin mixtures (AIN-93 Vitamin Mix), AIN-93 mineral mixture (AIN-93 Mineral Mix), L-cystine (L-cystine), two choline tartrate (cholinebitartrate), sucrose (sucrose), ground rice (rice flour), vegetable oil and beneficial heavy wool.Vegetable oil has the grease that similar aliphatic acid to beneficial heavy wool is formed available from the market product, but its saturated fatty acid content is lower.The grease of control group is from vegetable oil, and the grease of experimental group comes the bootstrap heavy wool, and the aliphatic acid of two kinds of edible oils is formed, and please see Table seven.
Table six: feed formula is formed
| Composition | Control group (percentage by weight) | Experimental group (percentage by weight) |
| Vegetable oil benefit heavy wool cholesterol ground rice casein gelatinization cornstarch sucrose cellulose mineral matter1Vitamin 2The two choline tartrates of L-cystine amount to | 14.3 - 0.2 35.0 17.3 13.2 10.0 5.0 3.5 1.0 0.3 0.2 100.0 | - 14.3 0.2 35.0 17.3 13.2 10.0 5.0 3.5 1.0 0.3 0.2 100.0 |
1AIN-93 mineral mixture (AIN-93 Mineral Mix).
2AIN-93 vitamin mixtures (AIN-93 Vitamin Mix).Table seven: the aliphatic acid of feed formula grease is formed
| Aliphatic acid | Vegetable oil (%) | Benefit heavy wool (%) |
| C16∶0 C16∶1 C18∶0 C18∶1 C18∶2 C18∶3 C18∶2/C18∶3 | 4.42 0.19 2.07 62.68 22.34 8.19 2.73 | 5.13 0.26 2.13 63.10 21.24 7.89 2.69 |
| SFAs MUFAs | 6.49 63.11 | 7.26 63.53 |
| PUFAs UFAs S∶P∶M(30％energy) | 30.53 93.64 1.9∶9.2∶18.9 | 29.13 92.66 2.2∶8.8∶19.0 |
The feed of control group and experimental group is formed and is prepared as shown in Table 6.With AIN-93 is the basal feed prescription, and its fat content respectively is adjusted into 14.3% vegetable oil group feed and 14.3% beneficial heavy wool group feed etc.Respectively add 0.2% cholesterol in addition and become high oily diet, produce high fat of blood, deduct in the percentage of its recruitment cornstarch from its former AIN-93 feed to cause animal used as test.Vitamin mixtures is then adopted and is not contained the vitamin E person.Be used as main carbohydrate source with ground rice, mainly contain carbohydrate 83.2%, protein 7.8%, fat 1.4% and a small amount of vitamin and mineral matter in the ground rice.So in feed was formed, protein was replaced by ground rice with the part source of fat.
The assay method of blood fat:
Desire is measured blood fat, needs first separation of serum albumen.Every sample that is separated into of serum lipoprotein is got 1mL serum and is placed the ultrahigh speed centrifuge tube, and adding 2mL density is the NaBr solution of 1.006g/mL, adds density again and be 1.097 NaBr solution 0.5mL.The sample total of gained is 3.5mL, and density is 1.019g/mL.Use Hitachi ultrahigh speed centrifuge S100AT6 Rotor at 4 ℃, with 453,000 * g centrifugal 3.5 hours.Taking-up floats over the part on centrifuge tube upper strata, and about 1mL is (VLDL+IDL).The solution of residue 2.5mL gets cumulative volume 3mL density 1.063g/mL solution at the NaBr solution 0.5mL that adds density 1.28g/mL.Under 4 ℃, with 453,000 * g centrifugal 3.5 hours, get 1mL upper strata liquid and be LDL.The LDL that obtains packs in the plastic centrifuge tube, keep in Dark Place behind the inflated with nitrogen 4 ℃ standby, finish every analysis in the Yu Santian.
Serum triglyceride utilizes enzyme process (GPO-PAP) to analyze.Get 10 μ L serum and add 1,000 μ L triglycerides reagent, after 5 minutes, make the triglycerides in the enzyme hydrolysis serum 37 ℃ of water-baths.Use spectrophotometer to measure down at last,, can get the concentration of TG after as calculated with the standard items comparison in wavelength 500nm.
Serum total cholesterol adopts enzyme to be color method (Enzymatic colorimetric method) and analyzes.Get 10 μ L serum and add 1,000 μ L cholesterol reagent, with 37 ℃ of water-baths after 5 minutes, with cholesterol esterlysis enzyme cholesterol and free fatty are decomposed in the cholesteryl ester hydrolysis, produce hydrogen peroxide with the cholesterol oxidase oxidation again, via peroxidase catalysis effect down, can produce red compound again.Measure its absorbance down in the 500nm wavelength,, learn the concentration of serum total cholesterol through conversion with the standard items comparison.
The serum high-density LP cholesterol is to utilize the principle of enzyme effect and colorimetric estimation to measure.Get quantitative serum earlier and add suitable precipitating reagent, with chylomicron (Chylomicrons), VLDL and LDL precipitation.Effect is centrifugal with it after completely, gets quantitative supernatant and adds the reagent of measuring cholesterol, measures light absorption value under wavelength 500nm, with the standard items comparison, can get the concentration of HDL-C after as calculated.
The serum low-density LP cholesterol is to utilize the principle of enzyme effect and colorimetric estimation to measure.Get the quantitative suitable precipitating reagent of serum adding LDL is precipitated, after the effect completely that it is centrifugal.Then get quantitative supernatant and add the reagent of measuring cholesterol, under wavelength 500nm, survey its light absorption value, compare with standard items.As calculated, again with T-CHOL value deduction supernatant cholesterol value, get final product the concentration of LDL-C.
Anti-oxidant force measurement:
The mensuration of red blood cell hemolysis time is with blood cell 2, and the mensuration of 2 '-Azobis (2-amidino-propane) Dihydrochloride AAPH is as the criterion.AAPH is a water-soluble substances, can be embedded in the middle mutually free radical that produces of fat.The generation that it can bring out hydroxyl free radical makes lipid peroxidation, causes the membrane structure instability.If this occur on the red blood cell, and hydroxyl free radical can be attacked the red blood cell film, and red blood cell is broken.Therefore can this pointer as the judgement oxidizing force.
The whole blood of 0.1mL added contain in the pipe of PBS, 37 ℃ of water-baths 5 minutes.The 1 gram AAPH 15mL that adds 1mL in the blood sample of 0.1mL is dissolved in 10mM Na
2HPO
4In the solution, rapid mixing is shaken with 90strokes/min again.After ten minutes, get 0.2mL solution in the PBS of 2.5mL, other gets 0.2mL solution in the water of 2.5mL, is put in the ice bath again.Then, in the time of 30,60,90,120,150,180 and 210 minutes, respectively the blood sample of 0.2mL is added among the PBS, under 4 ℃ centrifugal 10 minutes, placed room temperature 15 minutes, the light absorption value when surveying OD 540 with 362 * g.The red blood cell hemolysis time calculating formula of (min) is as follows:
Haemolysis percentage (hemolysis%)
=the light absorption value of sample in the PBS/light absorption value of sample * 100% in water
With time of reaching complete hemolysis percentage as hemolysis time (min).
The LDL oxidation lag time is to induce the lag phase of LDL oxidation to calculate with copper ion.The LDL that will not dialyse gets PBS and adjusts its concentration earlier, makes LDL-cholesterol concentration to 50 μ l/mL, is the Cu of 50 μ M again with final concentration
2+Carry out catalysed oxidn.Surveyed its light absorption value under 234nm then every 15 minutes, this promptly represents the growing amount of conjugated diene, and the oxidation curve that draws according to this, can try to achieve the lag time of inducing the LDL oxidation after as calculated.Lag time is defined as with the tangent line of the Propagation phase in the oxidation curve and the intercept of time and represents.
Experimental result:
The vegetable oil that is added in the feed of control group, its saturated fatty acid consists of 6.49%, and the beneficial heavy wool that preferred embodiment of the present invention provided that is added in the feed of experimental group, its saturated fatty acid consists of 7.26%.Hamster detects its lipid composition, as shown in Table 8 after the raising of 6 all high grease diet.
In all edible oils, the contained MUFAs of olive oil is the abundantest, up to about 78%, reaches about 13% but contain SFAs.Secondly the grease that is rich in MUFAs is the mustard caul-fat, still up to 63%, however its SFA only about 6.5%.2) and 8% linolenic acid (C18: essential fatty acid such as 3) another great advantage of mustard caul-fat is to contain 22% linolenic acid (C18:.The beneficial heavy wool that preferred embodiment of the present invention provided adds 12% olive oil in the mustard caul-fat, to increase the ratio of MUFAs.Can learn that from table eight the beneficial heavy wool that preferred embodiment of the present invention provided even compare with the lower vegetable oil of degree of saturation, still has the obvious effect that reduces triglyceride in the blood.
Table eight: hamster each blood fat biochemical values after six weeks of feeding different experiments feed.
| The feed group | Control group (mg/dL) | Experimental group (mg/dL) | Suppress percentage * |
| TG TC HDL-C LDL-C | 270±39 253±21 157±22 67±10 | 192±49 241±23 156±22 59±15 | -28.9％ b -4.7％ -0.6％ -11.9％ |
* (experimental group-control group)/control group * 100%.
bNumerical value is significantly different (p＜0.05).
Also inquired into the comparison of the resistance to oxidation of vegetable oil and beneficial heavy wool in this external experiment two, in respect of red blood cell hemolysis time and low-density lipoprotein (LDL) oxidation lag time binomial, gained the results are shown in table nine.Because the unsaturated oils of picked-up higher proportion, especially PUFAs (for example EPA or DHA) can make in interior many cell membranes of body and the lipoprotein to contain the PUFAs of more amount, and cause cell membrane and lipoprotein more oxidized, thereby be easy to generate free radical.So test two with the oxidized lag time of hemolysis time of RBC and LDL as its anti-oxidant pointer.Experimental result by table nine shows that beneficial heavy wool can increase the oxidized lag time of LDL significantly.
Table nine: red blood cell haemolysis and the comparison of low-density lipoprotein (LDL) oxidation lag phase time of hamster after six weeks of feeding different experiments feed.
| The feed group | Control group (min) | Experimental group (min) | Prolong percentage * |
| Red blood cell hemolysis time LDL oxidation lag time | 119±25 a 155.3±33.1 b | 133±21 a 213.8±45.5 a | +11.8％ +37.7％ b |
* (experimental group-control group)/control group * 100%.
bNumerical value is significantly different (p＜0.05).
By the invention described above preferred embodiment as can be known, no matter preferred embodiment of the present invention is compared with saturated fatty acid content the higher person (experiment one) or junior's (experiment two), all have the effect that reduces triglyceride in the blood, thereby can reduce the generation probability of angiocardiopathy.In addition, in two experiments, find also to have the effect that the LDL oxidation lag time is prolonged respectively, and regulate the effect of TC and LDL-C concentration in the blood.
Though the present invention discloses as above with a preferred embodiment; but it is not in order to limit the present invention; anyly have the knack of this skill person; without departing from the spirit and scope of the present invention; when can being used for a variety of modifications and variations, so protection scope of the present invention defines and is as the criterion when looking accompanying claim.
Claims (10)
1, a kind of edible oil with reduction blood fat effect, this edible oil comprises at least:
One first vegetable oil; And
One second vegetable oil,
A blending edible oil that is mixed by this first vegetable oil and this second vegetable oil contains the unrighted acid of 85 percentage by weights at least, and this blending edible oil contains the saturated fatty acid of 6-10 percentage by weight, the polybasic unsaturated fatty acid of 26-32 percentage by weight and the monounsaturated fatty acid of 59-69 percentage by weight.
2, the edible oil with reduction blood fat effect according to claim 1, it is characterized in that, this monounsaturated fatty acid comprises oleic acid (18: 1), and this polybasic unsaturated fatty acid comprises linolenic acid (18: 2) and linolenic acid (18: 3), and this blending edible oil contains the oleic acid (18: 1) of 58-68 percentage by weight, the linolenic acid (18: 2) of 19-24 percentage by weight and the linolenic acid (18: 3) of 6-9 percentage by weight.
3, the edible oil with reduction blood fat effect according to claim 1 is characterized in that this first vegetable oil is an olive oil.
4, the edible oil with reduction blood fat effect according to claim 1 is characterized in that this second vegetable oil is the mustard caul-fat.
5, according to claim 1 have an edible oil that reduces the blood fat effect, it is characterized in that, when this first vegetable oil is olive oil and this second vegetable oil when being the mustard caul-fat, the mixed proportion of olive oil and mustard caul-fat is 12: 88.
6, tool according to claim 1 reduces the edible oil of blood fat effect, it is characterized in that, this polybasic unsaturated fatty acid comprises linolenic acid (18: 2) and linolenic acid (18: 3), and the ratio of the linolenic acid of this polybasic unsaturated fatty acid and linolenic acid is 2-3.
7, the edible oil with reduction blood fat effect according to claim 6 is characterized in that it is 2-3: 8-9: 18-20 that wherein contained saturated fatty acid, polybasic unsaturated fatty acid and monounsaturated fatty acid account for the ratio of absorbing heat 30% every day.
8, the edible oil with reduction blood fat effect according to claim 6 is characterized in that this first vegetable oil is an olive oil.
9, the edible oil with reduction blood fat effect according to claim 6 is characterized in that this second vegetable oil is the mustard caul-fat.
10, according to claim 6 have an edible oil that reduces the blood fat effect, it is characterized in that, when this first vegetable oil is olive oil and this second vegetable oil when being the mustard caul-fat, the mixed weight ratio of olive oil and mustard caul-fat is 12: 88.
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| CNB031029833A CN1307889C (en) | 2003-01-24 | 2003-01-24 | Edible oil possessing efficacy of lowering down blood fat |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB031029833A CN1307889C (en) | 2003-01-24 | 2003-01-24 | Edible oil possessing efficacy of lowering down blood fat |
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| CN1307889C true CN1307889C (en) | 2007-04-04 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5169669A (en) * | 1991-09-25 | 1992-12-08 | The Procter & Gamble Company | Cooking oils |
| CN1181194A (en) * | 1996-10-18 | 1998-05-13 | 花王株式会社 | General-purpose oils composition |
| CN1448061A (en) * | 2002-03-29 | 2003-10-15 | 南海油脂工业(赤湾)有限公司 | Plant mixed oil with rational proportion of fatty acid |
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2003
- 2003-01-24 CN CNB031029833A patent/CN1307889C/en not_active Expired - Lifetime
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5169669A (en) * | 1991-09-25 | 1992-12-08 | The Procter & Gamble Company | Cooking oils |
| CN1181194A (en) * | 1996-10-18 | 1998-05-13 | 花王株式会社 | General-purpose oils composition |
| CN1448061A (en) * | 2002-03-29 | 2003-10-15 | 南海油脂工业(赤湾)有限公司 | Plant mixed oil with rational proportion of fatty acid |
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