CN1305999A - Process for extracting taxol from filtrate of enqlish yew cell culturing liquid - Google Patents
Process for extracting taxol from filtrate of enqlish yew cell culturing liquid Download PDFInfo
- Publication number
- CN1305999A CN1305999A CN 01107410 CN01107410A CN1305999A CN 1305999 A CN1305999 A CN 1305999A CN 01107410 CN01107410 CN 01107410 CN 01107410 A CN01107410 A CN 01107410A CN 1305999 A CN1305999 A CN 1305999A
- Authority
- CN
- China
- Prior art keywords
- taxol
- nutrient solution
- filtrate
- resin
- absorption
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
The resin title | Amount of resin (dry weight) (g) | Taxol amount (mg/ml) in the test solution | Taxol residual quantity (mg) in the test solution | Adsorptive capacity (mg) | 1 gram resin absorption amount (mg) | Elution amount (mg) | Eluting rate (%) |
???S-8 | ??0.13 | ?2.12mg/12ml | ?????0.9562 | ??1.1638 | ?????8.9 | ???0.978 | ????84.03 |
???AB-8 | ??0.22 | ?2.136mg/40ml | ?????0.2792 | ??1.8568 | ?????8.4 | ???1.7436 | ????93.90 |
???X-5 | ??0.20 | ?2.165mg/40ml | ?????0.4488 | ??1.7162 | ?????7.8 | ???1.6820 | ????98.0 |
???XAD-4 | ??0.58 | ?3.576mg/50m1 | ?????0.291 | ??3.285 | ?????5.66 | ???3.125 | ????95.59 |
???D4020 | ??0.20 | ?2.136mg/40ml | ?????0.621 | ??1.515 | ?????7.58 | ???1.423 | ????93.92 |
???NKA-9 | ??0.15 | ?0.9381mg/10ml | ?????0.0693 | ??0.8688 | ?????5.79 | ???0.815 | ????93.81 |
???LD605 | ??0.30 | ?1.4145mg/20ml | ?????0.190 | ??1.2245 | ?????4.08 | ???1.181 | ????96.44 |
The resin title | Amount of resin (dry weight) (g) | Taxol amount (mg/ml) in the test solution | Taxol residual quantity (mg) in the test solution | Adsorptive capacity (mg) | Elution amount (mg) | Eluting rate (%) | The rate of recovery (%) |
??S-8 | ???5.44 | ????2.52mg/400ml | ????0.1 | ??2.42 | ??2.40 | ??99.2 | ??95.2 |
??AB-8 | ???0.82 | ????0.325mg/100ml | Do not detect | ??0.325 | ??0.3172 | ??97.6 | ??97.6 |
??X-5 | ???0.39 | ????0.1625mg/50ml | Do not detect | ??0.1625 | ??0.1368 | ??84.2 | ??84.2 |
??XAD-4 | ???2.3 | ????1.26mg/200ml | ????0.376 | ??0.889 | ??0.831 | ??93.5 | ??65.95 |
??D4020 | ???0.25 | ????0.2255mg/50ml | Do not detect | ??0.2255 | ??0.2230 | ??98.89 | ??98.89 |
??NKA-9 | ???1.68 | ????1.26mg/200ml | ????0.123 | ??1.134 | ??1.122 | ??98.9 | ??82.69 |
??LD605 | ???0.15 | ????0.1625mg/50ml | Do not detect | ??0.1625 | ??0.1585 | ??97.54 | ??97.54 |
The resin title | Taxol amount (mg) in the test solution | Adsorptive capacity (mg) | Elution amount (mg) | Eluting rate (%) | The rate of recovery (%) |
????X-5 | ?????0.76 | ??0.76 | ?????0.703 | ????92.5 | ????92.5 |
?AB-8 | ?????0.76 | ??0.76 | ?????0.70 | ????92.1 | ????92.1 |
Claims (8)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB011074108A CN1166655C (en) | 2001-01-03 | 2001-01-03 | Process for extracting taxol from filtrate of enqlish yew cell culturing liquid |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB011074108A CN1166655C (en) | 2001-01-03 | 2001-01-03 | Process for extracting taxol from filtrate of enqlish yew cell culturing liquid |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1305999A true CN1305999A (en) | 2001-08-01 |
CN1166655C CN1166655C (en) | 2004-09-15 |
Family
ID=4656338
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB011074108A Expired - Fee Related CN1166655C (en) | 2001-01-03 | 2001-01-03 | Process for extracting taxol from filtrate of enqlish yew cell culturing liquid |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN1166655C (en) |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1298712C (en) * | 2004-12-27 | 2007-02-07 | 梅县梅雁生物工程研究所 | Method for elementary separating 10-noracetyl Baccatins III from branches and leaves of yew |
WO2008151526A1 (en) * | 2007-06-08 | 2008-12-18 | Dalian Institute Of Chemical Physics, Chinese Academy Of Sciences | A method of inducing taxane production |
CN101319199B (en) * | 2007-06-08 | 2010-10-27 | 中国科学院大连化学物理研究所 | Method for cell abduction generation of taxone with Chinese yew |
CN101314597B (en) * | 2008-07-04 | 2010-12-01 | 华中科技大学 | Method for separating paclitaxel from yew cell suspending culture solution |
CN101177421B (en) * | 2006-11-07 | 2010-12-15 | 上海天伟生物制药有限公司 | Method for preparing high-purity taxone compounds |
CN102417492A (en) * | 2011-12-07 | 2012-04-18 | 福建紫杉园生物有限公司 | Method for separating and purifying paclitaxel |
CN102653528A (en) * | 2012-04-19 | 2012-09-05 | 重庆市碚圣农业科技股份有限公司 | Method for separating purified paclitaxel and 10 diaminobenzidine (DAB) III by utilizing macroporous resin |
CN103570647A (en) * | 2013-11-06 | 2014-02-12 | 湖南农业大学 | Method for preparing high-purity paclitaxel from taxus chinensis cell culture fluid |
-
2001
- 2001-01-03 CN CNB011074108A patent/CN1166655C/en not_active Expired - Fee Related
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1298712C (en) * | 2004-12-27 | 2007-02-07 | 梅县梅雁生物工程研究所 | Method for elementary separating 10-noracetyl Baccatins III from branches and leaves of yew |
CN101177421B (en) * | 2006-11-07 | 2010-12-15 | 上海天伟生物制药有限公司 | Method for preparing high-purity taxone compounds |
WO2008151526A1 (en) * | 2007-06-08 | 2008-12-18 | Dalian Institute Of Chemical Physics, Chinese Academy Of Sciences | A method of inducing taxane production |
CN101319199B (en) * | 2007-06-08 | 2010-10-27 | 中国科学院大连化学物理研究所 | Method for cell abduction generation of taxone with Chinese yew |
CN101314597B (en) * | 2008-07-04 | 2010-12-01 | 华中科技大学 | Method for separating paclitaxel from yew cell suspending culture solution |
CN102417492A (en) * | 2011-12-07 | 2012-04-18 | 福建紫杉园生物有限公司 | Method for separating and purifying paclitaxel |
CN102653528A (en) * | 2012-04-19 | 2012-09-05 | 重庆市碚圣农业科技股份有限公司 | Method for separating purified paclitaxel and 10 diaminobenzidine (DAB) III by utilizing macroporous resin |
CN102653528B (en) * | 2012-04-19 | 2014-07-16 | 重庆市碚圣农业科技股份有限公司 | Method for separating purified paclitaxel and 10 diaminobenzidine (DAB) III by utilizing macroporous resin |
CN103570647A (en) * | 2013-11-06 | 2014-02-12 | 湖南农业大学 | Method for preparing high-purity paclitaxel from taxus chinensis cell culture fluid |
Also Published As
Publication number | Publication date |
---|---|
CN1166655C (en) | 2004-09-15 |
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Free format text: CORRECT: ADDRESS; FROM: 514787 3/F, MEIYAN INDUSTRIAL BUILDING, JIAYING WEST ROAD, MEIXIAN COUNTY, GUANGDONG PROVINCE TO: 514031 SHANGHUANGTANG, HUANSHI WEST ROAD, MEIZHOU CITY, GUANGDONG PROVINCE |
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Effective date of registration: 20101231 Address after: 514031, on the west side of Ring Road, Meizhou, Guangdong Patentee after: Guangdong Qingfa Pharmaceutical Industry Co., Ltd. Address before: 514787 Guangdong province Meixian Jiaying West Industrial Meiyan building on the third floor Patentee before: Meiyan Inst. of Biological Engineering, Meixian County |
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Owner name: GUANGDONG KELUN PHARMACEUTICAL CO., LTD. Free format text: FORMER NAME: GUANGDONG QINGFA PHARMACEUTICAL INDUSTRY CO., LTD. |
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Address after: 514031, on the west side of Ring Road, Meizhou, Guangdong Patentee after: GUANGDONG KELUN PHARMACEUTICAL CO., LTD. Address before: 514031, on the west side of Ring Road, Meizhou, Guangdong Patentee before: Guangdong Qingfa Pharmaceutical Industry Co., Ltd. |
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CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20040915 Termination date: 20190103 |
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CF01 | Termination of patent right due to non-payment of annual fee |