CN1296851A - Antivirus immune globulin for dog, fox and marten and its preparing process - Google Patents
Antivirus immune globulin for dog, fox and marten and its preparing process Download PDFInfo
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- CN1296851A CN1296851A CN 00123292 CN00123292A CN1296851A CN 1296851 A CN1296851 A CN 1296851A CN 00123292 CN00123292 CN 00123292 CN 00123292 A CN00123292 A CN 00123292A CN 1296851 A CN1296851 A CN 1296851A
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- Prior art keywords
- dog
- vulpes
- ermine
- immune globulin
- horse
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Abstract
An antivirus immune globulin for dog, fox and marten to prevent artificial infection of viral infections diseases between animals is prepared through concentrating the low virulent culture liquid of infectious viruses, adding auxiliary agent, immunizing horse, donkey or mule, collecting blood when the agar diffusion potency of globulin in serum reaches the maximum, separating plasm, and extracting said immune globulin by octanoic acid method. Its advantages are high safety and potency and storage at 4-12 deg.C or -20-0 deg.C.
Description
The invention discloses a kind of dog, Vulpes, ermine with antivirus immune globulin and production technology, relate to animal and use the biologics production technical field.
In the fur economic animal, main viral infectious is canine distemper, minute viral enteritis, rabies, dog hepatitis (epizootic fox encephalitis), dog laryngotracheitis, dog parainfluenza, pseudorabies and coronavirus enteritis etc., particularly the M ﹠ M of canine distemper and parvovirus enteritis is all very high, causes very big loss for the breed of fur economic animal; Conventional way is the Vaccination and Immunoprophylaxis that carries out viral vaccine, but the currently available vaccines, existing vaccines protective rate is not high, can only reach about 80%, and immune effect is undesirable.Viral disease and other infectious disease are different, because the particularity of virus attack and growing multiplication promptly must be bred in the target cell that lives, so till now, also do not find a kind of medicine of the internal specific of cell in vivo kill virus.
The invention provides a kind of dog, Vulpes, ermine with antivirus immune globulin and production technology, utilize horse, donkey and mule to produce dog, Vulpes, ermine and use antivirus immune globulin, purpose is to solve the urgent prevention of fur economic animal viral infectious and the problem of emergency treatment.
Technical solution of the present invention is as follows: the weak malicious culture fluid of dog, Vulpes, ermine virus spread (at least a) is concentrated the TCID of virus
50Tire 10
5.0More than, through the differential centrifugation method virus is purified concentrated 50～120 times, add the Fu Shi Freund's complete adjuvant, by optimal path and metering after 3 times horse (or donkey, mule) being carried out immunity, treat to take a blood sample when antibody titer reaches peak-peak, separation of serum is with the antiviral specific immune globulin of sad method separation and Extraction purification, be IgG, content 20mg/ml (± 2%).
Good effect of the present invention is: by horse, donkey, mule immunity, prevented the artificial infection of some other viral infectious between the fur economic animal of the same race, safety is good, the neutralization of the antivirus immune globulin that dog, Vulpes and the ermine that produces with horse used tire for: it is 10 that the neutralization of the immunoglobulin of 100 times of dilutions is tired
3.4It is 10 that (promptly 2512 times), the neutralization of the immunoglobulin of 200 times of dilutions are tired
23(promptly 199 times).Extract immunoglobulin by serum, be colourless transparent liquid, both can stored frozen (more than 20 ℃), also can stored refrigerated (+4 ℃～+ 12 ℃) accumulating convenient.
Better at clinical trial result, this product has been carried out urgent prevention and emergency treatment test in the toy outpatient service, 5 diarrhoea has taken place in the same group dog, detect through clinical diagnosis and Electronic Speculum, all be diagnosed as minute viral enteritis, all the other 54 pups that do not fall ill have been carried out urgent protective inoculation with antiviral bigeminy immunoglobulin (anti-canine distemper and anti-parvovirus), enteritis has not taken place again through observing.32 routine canine distemper are carried out emergency treatment, cure 29 examples, cure rate reaches 90.6%, and the sick dog of 43 routine parvovirus enteritis has been carried out emergency treatment, cures 40 examples, and cure rate reaches 93%.
Further specify the present invention below in conjunction with embodiment:
Embodiment 1
With canine distemper attenuated culture fluid (TCID
50Tire 10
5.625More than) and the weak malicious culture fluid (TCID of parvovirus
50Tire 10
5.0More than) use the freezing ultra-lowing centrifuge differential centrifugation, two kinds of viruses are purified concentrate 100 times respectively.Equivalent mixed liquor and Fu Shi Freund's complete adjuvant equivalent with two kinds of viruses are made immunoreagent, give healthy horse immunity inoculation.Fundamental immunity for the first time, intradermal vaccination, divides 8 injections by 8ml/; Booster immunization for the second time, the lymph node inoculation, divides 8 injections by 8ml/; Hyperimmunization for the third time, vein is slowly injected, and 3ml/, in one week of two minor ticks, last immunity blood sampling in back about 14 days detects antibody titer, treats that agar diffusion tires when reaching more than 32 times blood-letting and separation of serum under aseptic condition.In serum, add the doubly acetate buffer of the 0.06M/L of the pH4.0 of amount, the powerful stirring under the room temperature, calculate by serum amount simultaneously, ratio with 7.5% drips the sad of chemical pure level, 4000 rev/mins centrifugal 30 minutes, cross the leaching supernatant bag filter of packing into, dialysis is 48 hours in the phosphate buffer of the 0.015M/L of pH7.4, reuse germ tight filter sucking filtration, sampling detects agar diffusion and tires more than 16 times the time, protein content in the sterilizing room packing, promptly gets product of the present invention when 20mg/ml (± 2%).
Embodiment 2
With canine distemper attenuated culture fluid (TCID
50Tire 10
5.625More than), the weak malicious culture fluid (TCID of parvovirus enteritis
50Tire 10
5.0More than), the adenovirus attenuated culture fluid (TCID of dog hepatitis (epizootic fox encephalitis)
50Tire 10
4.5More than), the weak malicious culture fluid (TCID of canine parainfluenza virus
50Tire 10
4.5More than), the weak malicious culture fluid (LD of rabies virus
50Tire 10
5.0More than) and pseudo-rabies viurs attenuated strain culture fluid (TCID
50Tire 10
5.0More than) malicious culture fluid a little less than six kinds, purifying concentrates, and donkey or mule are carried out immunity, by described in the embodiment 1 the method rules are carried out, promptly get product of the present invention.
Embodiment 3
The kind of the weak malicious culture fluid of changeable is undertaken by the method rules of narration among the embodiment 1 according to actual needs, just can obtain the antivirus immune globulin that corresponding dog Vulpes ermine is used.
Claims (5)
1, a kind of dog, Vulpes, ermine are used antivirus immune globulin, it is characterized in that: produce dog, Vulpes, ermine with horse or donkey, mule through immunity and use antivirus immune globulin.
2, a kind of dog, Vulpes, ermine are used the production technology of antivirus immune globulin, comprise that the weak malicious culture fluid with dog, Vulpes, ermine virus spread concentrates, add the Fu Shi Freund's complete adjuvant, horse or donkey or mule are carried out immunity, treat that the agar diffusion of globulin is tired in the serum and take a blood sample when reaching summit, separated plasma is with sad method separation and Extraction immunoglobulin.
3, production technology according to claim 2 is characterized in that: take Intradermal, acupuncture point, vein to divide three inoculations when immune horse or donkey or mule, per one week of two minor ticks.
4, production technology according to claim 2 is characterized in that: 50～120 times of the weak malicious culture fluid simmer down tos of dog, Vulpes, ermine virus spread.
5, production technology according to claim 2 is characterized in that: the weak malicious culture fluid of dog, Vulpes, ermine virus spread comprises one or more mixture in canine distemper, minute viral enteritis, rabies, dog hepatitis (epizootic fox encephalitis), dog laryngotracheitis, dog parainfluenza, pseudorabies and the coronavirus enteritis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00123292 CN1296851A (en) | 2000-11-18 | 2000-11-18 | Antivirus immune globulin for dog, fox and marten and its preparing process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00123292 CN1296851A (en) | 2000-11-18 | 2000-11-18 | Antivirus immune globulin for dog, fox and marten and its preparing process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1296851A true CN1296851A (en) | 2001-05-30 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00123292 Pending CN1296851A (en) | 2000-11-18 | 2000-11-18 | Antivirus immune globulin for dog, fox and marten and its preparing process |
Country Status (1)
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|---|---|
| CN (1) | CN1296851A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100569287C (en) * | 2004-02-10 | 2009-12-16 | 中国人民解放军军事医学科学院野战输血研究所 | Dog immunoglobulin'intravenous, its preparation method and dog intravenous immune globulin preparation |
| CN104784689A (en) * | 2015-05-05 | 2015-07-22 | 王宏伟 | Canine parvovirus specific immune globulin preparation as well as preparation method and application thereof |
-
2000
- 2000-11-18 CN CN 00123292 patent/CN1296851A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100569287C (en) * | 2004-02-10 | 2009-12-16 | 中国人民解放军军事医学科学院野战输血研究所 | Dog immunoglobulin'intravenous, its preparation method and dog intravenous immune globulin preparation |
| CN104784689A (en) * | 2015-05-05 | 2015-07-22 | 王宏伟 | Canine parvovirus specific immune globulin preparation as well as preparation method and application thereof |
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| SE01 | Entry into force of request for substantive examination | ||
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |