CN1289532C - Post extraction production technology of pullulanose - Google Patents
Post extraction production technology of pullulanose Download PDFInfo
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- CN1289532C CN1289532C CN 200510042018 CN200510042018A CN1289532C CN 1289532 C CN1289532 C CN 1289532C CN 200510042018 CN200510042018 CN 200510042018 CN 200510042018 A CN200510042018 A CN 200510042018A CN 1289532 C CN1289532 C CN 1289532C
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- Prior art keywords
- pulullan
- pullulanose
- production process
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- fermented liquid
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Abstract
The present invention relates to a post extraction production process of pullulanose, which belongs to the extraction production process of pullulanose. The post extraction production process is characterized in that The fermentation liquid of the pullulanose in which bacteria are removed is decolorized, desalted and concentrated by a film filtration technique, and a traditional dehydration extraction method for a polysaccharide organic solvent is discarded. The fermentation liquid of the pullulanose after decolorization, desalinization and concentration is directly dried by a fluidized bed drying technique. An extraction yield is greater than 95%. The fermentation liquid has good quality. Security, continuity and automation can be realized in a production process. The post extraction production process provides raw material sources for the wide application of the pullulanose.
Description
Technical field
The present invention relates to the suitability for industrialized production technology of pulullan, specifically relate to a kind of post extraction production technology of pulullan.
Background technology
Pulullan is the higher Microbial exopolysaccharides of a kind of using value, and by α-1, the trisaccharide maltose repeating unit that the 4-glycosidic link connects is through α-1, the straight chain shape polysaccharide that the 6-glycosidic link is polymerized.But because the difference of bacterial strain uses therefor, the trisaccharide maltose unit in the polysaccharide chain once in a while also can be by the minute quantity maltotetrose, and maltose or glucosyl residue replace, and also occur branched structure under the individual cases.The molecular weight of pulullan is generally 5.0 * 10
4-5.0 * 10
6Between the daltons; Its molecular weight size is subjected to the influence of culture condition bigger.The control fermentation condition can be fixed its molecular weight in certain scope.The research work of pulullan originates in West Germany, and the Englishman has also done many work aspect theoretical.Japan compares the especially research of production technique and product application of system.China has just begun relevant research at the initial stage eighties, though obtained certain achievement at bacterial classification and process aspect, manufacturers produce is not arranged yet.At present, the post extraction production technology of polysaccharide (coagulating polysaccharide, gelling gum as xanthan gum, heat) all dewaters to polysaccharide fermentation liquid as dewatering agent with organic solvents such as alcohol, Virahols, and simultaneously to product decolouring and desalination, dry then, owing to will use a large amount of organic solvents in process of production, therefore the distillation of organic solvent is reclaimed needs to consume a large amount of steam, and because these organic solvents all belong to inflammable explosive article, there is great unsafe factor in production process.
Summary of the invention
The object of the present invention is to provide a kind of novel pulullan post extraction production technology that is different from traditional polysaccharide extractive technique, adopt operational path of the present invention to produce pulullan, product yield height, quality is good, production process can realize safe, serialization and automatization, and production cost is significantly less than traditional extraction technology.
Technical scheme of the present invention is that the post extraction production technology of this pulullan is characterized in that comprising the steps:
(1), adopt tubular fibre or flat plate ultrafiltration membrane separator to the pulullan fermented liquid decolour, desalination and concentrating: described ultra-filtration process is after the pulullan fermented liquid is removed thalline through filter, clear filtrate is pumped into the membrane separation apparatus from basin, the molecular weight cut-off of film is 2000-15000D, trapped fluid returns in the basin, water, pigment and inorganic salt see through film, in this process, add deionized water to basin continuously, up to standard until colourity and salinity, proceed ultrafiltration, up to standard up to concentration;
(2), adopt the fluidized-bed convection drying: with decolouring, desalination and the fermented liquid after concentrating directly adopt fluidised bed drying to obtain product.
The ultra-filtration membrane separator that the present invention adopts can be commercial tubular fibre or flat plate ultrafiltration membrane separator, also can make up the various forms of membrane separation apparatuss that ultra-filtration membrane that processing made by various materials is formed voluntarily.The aperture of film be reference can see through pigment and penetrating amount, but must hold back the pulullan molecule, is generally 2000-15000D.
Fluidised bed drying technology of the present invention can be the drying that the fluid bed dryer of the various models of employing carries out the pulullan fermented liquid.
The present invention extracts the pulullan technology with organic solvent method and compares, and beneficial effect is:
1, adopt the decolouring of membrane filtration technology, desalination and concentrating to avoid in the production process secondary pollution that produces because of conventional activated carbon decolorizing and ion-exchange desalination, reduce energy consumption.
2, adopt fluidization convection drying product, can avoid the potential danger factor that causes because of solvent (ethanol, Virahol) volatilization in the common process production process, and can save because of organic solvent and reclaim a large amount of steam that distillation is consumed.
3, pulullan post extraction production technology of the present invention, owing to abandoned organic solvent, utilize the membrane filtration technology to the pulullan fermented liquid of removing thalline decolour, desalination and concentrate, and adopt fluidization that fermented liquid is carried out convection drying, not only saved a large amount of energy, and production process is safe and reliable.
4, pulullan post extraction production technology of the present invention, product yield height, extract yield are greater than 95%, and quality is good, and production process can realize safe, serialization and automatization.
Further specify below by the technology of example invention:
Embodiment
The post extraction production technology of pulullan: as shown in the figure.
1, the pulullan fermented liquid is removed thalline through filter, and clear liquid pumps into filtrate tank.
2, with the clarification pulullan fermented liquid (7m that removes thalline in the basin
3) pump into the hollow fiber ultrafiltration membrane separator, the molecular weight cut-off of film is 6000D, trapped fluid returns in the basin, water, pigment and inorganic salt see through film, in this process, add deionized water to basin continuously, the additional amount of deionized water for see through liquid long-pending 50%, when colourity and salinity are up to standard, stop to add deionized water; Proceed ultrafiltration, reach 20% up to concentration.
3, concentrate good pulullan solution pump and go into fluidized-bed, drying obtains the 430Kg sample.Extract yield 97.8%.
4, by after the drying, usual method is pulverized, packing, gets product.
Claims (1)
1, the post extraction production technology of pulullan is characterized in that comprising the steps:
(1), adopt tubular fibre or flat plate ultrafiltration membrane separator to the pulullan fermented liquid decolour, desalination and concentrating: described ultra-filtration process is after the pulullan fermented liquid is removed thalline through filter, clear filtrate is pumped into the membrane separation apparatus from basin, the molecular weight cut-off of film is 2000-15000D, trapped fluid returns in the basin, water, pigment and inorganic salt see through film, in this process, add pure water to basin continuously, up to standard until colourity and salinity, proceed ultrafiltration, up to standard up to concentration;
(2), adopt the fluidized-bed convection drying: with decolouring, desalination and the fermented liquid after concentrating directly adopt fluidised bed drying to obtain product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510042018 CN1289532C (en) | 2005-01-11 | 2005-01-11 | Post extraction production technology of pullulanose |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510042018 CN1289532C (en) | 2005-01-11 | 2005-01-11 | Post extraction production technology of pullulanose |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1651468A CN1651468A (en) | 2005-08-10 |
| CN1289532C true CN1289532C (en) | 2006-12-13 |
Family
ID=34876304
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200510042018 Expired - Fee Related CN1289532C (en) | 2005-01-11 | 2005-01-11 | Post extraction production technology of pullulanose |
Country Status (1)
| Country | Link |
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| CN (1) | CN1289532C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10568839B2 (en) | 2011-01-11 | 2020-02-25 | Capsugel Belgium Nv | Hard capsules |
| US11878079B2 (en) | 2017-04-14 | 2024-01-23 | Capsugel Belgium Nv | Pullulan capsules |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100465191C (en) * | 2006-11-03 | 2009-03-04 | 天津市工业微生物研究所 | Drying method of bearing blue polysaccharide |
| CN101555507B (en) * | 2009-05-18 | 2012-03-28 | 杨凌壹之农微生物工程技术研究院有限公司 | Method for using pulullan to prepare high-purity maltotriose |
| CN102617748B (en) * | 2012-04-06 | 2016-12-14 | 华远医药研究院有限公司 | A kind of purification process of pulullan polysaccharide |
| CN103172757B (en) * | 2012-12-31 | 2015-01-14 | 天津北洋百川生物技术有限公司 | Extracting process of pulullan polysaccharide |
| JP7222911B2 (en) | 2017-04-14 | 2023-02-15 | カプスゲル・ベルギウム・ナムローゼ・フェンノートシャップ | How to make pullulan |
| CN110638767B (en) * | 2019-10-12 | 2021-09-10 | 山东省食品发酵工业研究设计院 | Vitamin E solid tablet and preparation method thereof |
-
2005
- 2005-01-11 CN CN 200510042018 patent/CN1289532C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10568839B2 (en) | 2011-01-11 | 2020-02-25 | Capsugel Belgium Nv | Hard capsules |
| US11878079B2 (en) | 2017-04-14 | 2024-01-23 | Capsugel Belgium Nv | Pullulan capsules |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1651468A (en) | 2005-08-10 |
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Owner name: SHANDONG ZHONG QING BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: SU LI; LAN WENZHONG Effective date: 20080613 |
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Effective date of registration: 20080613 Address after: No. 33, Yong Xi Road, Linzi District, Shandong, Zibo: 255400 Patentee after: Shandong Zhong Qing Biological Technology Co., Ltd. Address before: No. 41 Jiefang Road, Lixia District, Shandong City, Ji'nan Province, China: 250013 Co-patentee before: Lan Wenzhong Patentee before: Su Li |
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Granted publication date: 20061213 Termination date: 20140111 |