CN1285281C - Preparation of bitter gourd seed aril lycopene powder - Google Patents
Preparation of bitter gourd seed aril lycopene powder Download PDFInfo
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- CN1285281C CN1285281C CNB2004100006451A CN200410000645A CN1285281C CN 1285281 C CN1285281 C CN 1285281C CN B2004100006451 A CNB2004100006451 A CN B2004100006451A CN 200410000645 A CN200410000645 A CN 200410000645A CN 1285281 C CN1285281 C CN 1285281C
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- lycopene
- bitter melon
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Abstract
The present invention relates to a method for making bitter melon seed aril lycopene powder, which has the technical scheme that ripe and fresh bitter melon seed arils are naturally fermented at 20 to 45 DEG C or fermented in an anaerobic mode with the pH value of smaller than 7 by adding saccharomycete for 8 to 72 hours; starch auxiliary materials and the arils in the proportion of 1:70 to 1:7 by weight are thoroughly and uniformly stirred and mixed so as to be homogenized; the mixture is dewatered in a filter pressing mode and then dried; the dried mixture is pulverized so as to obtain the bitter melon seed aril lycopene powder. The content of the lycopene in the bitter melon seed aril lycopene powder prepared by the method of the present invention can reach 0.1 to 1%. The bitter melon seed aril lycopene powder can be directly developed into food or lycopene health food. In addition, the bitter melon seed aril lycopene powder does not have the problem of solvent residue; the method of the present invention has the advantage of low production cost.
Description
Technical field
The present invention relates to directly the aril of cucurbitaceous plant balsam pear is processed as the preparation method of bitter melon seed aril lycopene powder.
Background technology
Lycopene is a kind of of carotenoid, has unique physiological hygiene function, the generation of anti-cancer and tumour effectively in advance, and can prevent to damage with repair cell.Lycopene mainly is present in the ripening fruits of plant of Solanaceae, as tomato, balsam pear etc.The research that existing lycopene extracts, major part is at the plant tomato.Abroad be as raw material, with CO with whole tomato
2Critical extraction extracts, and shortcoming is that scale of investment is big, the production cost height; Chinese patent CN198661, CN1260990 and CN1358801A also disclose and have used plant tomato, catsup and tomato skin etc. as raw material, extract lycopene method.But the content of lycopene in the plant tomato is less relatively, and the content of lycopene in the ripe tomato only is 3～14mg/100g; And content of lycopene can reach 20～60mg/100g in the ripe bitter melon seed aril, but still seldom, it also is very uneconomical directly extracting lycopene with the bitter melon seed aril.
Summary of the invention
Technical problem to be solved by this invention provides the preparation method of the high bitter melon seed aril lycopene powder of a kind of content of lycopene.
The technical scheme that this preparation method adopts is: at first, get ripe bright bitter melon seed aril and carry out spontaneous fermentation or add saccharomycete under PH＜7 conditions carrying out anaerobic fermentation at 20～45 ℃, fermentation time is 8～72 hours; With the starch based auxiliary material: aril=1: 70～1: 7 (Wt) ratio adds the abundant stirring and evenly mixing of starch based auxiliary material, homogeneous; Subsequently, carry out filter-press dehydration, drying; At last, obtain bitter melon seed aril lycopene powder through pulverizing.
Because sugar is very high in the bitter melon seed aril, if do not remove sugar as far as possible, then sugar can wrap up lycopene crystal, thereby be unfavorable for the extraction of lycopene, therefore, the present invention has adopted zymotechnique, preferred 30～35 ℃ of scopes of above-mentioned fermentation temperature, preferred 23～25 hours of fermentation time.
When adding saccharomycete carry out anaerobic fermentation under PH＜7 conditions, this saccharomycete can adopt known saccharomycete, for example: saccharomyces formosensis bacterium No. 396 (F-396), Cuba I saccharomycete (having another name called the As.2.1189 saccharomycete), Basse R word saccharomycete etc.
Above-mentioned starch based auxiliary material can adopt tapioca, cornstarch, potato starch etc., the addition of starch based auxiliary material hour, the aril lycopene powder is easily conglomeration, caking in organic solvent, is unfavorable for the extraction of lycopene, and is same, when addition is too much, lycopene powder content in the unit mass reduces, and also is unfavorable for the extraction of lycopene, therefore, suitable auxiliary material addition is the starch based auxiliary material: aril=1: 70～1: 7 (Wt) scope, most preferably 1: 60～1: 40.
Above-mentioned drying means can adopt vacuum drying or freeze-drying, and when adopting vacuum drying, the vacuum drying temperature is more satisfactory smaller or equal to 60 ℃; The pulverized particles degree gets final product at 40～80 orders.
Can be by the content of lycopene in the bitter melon seed aril lycopene powder of the inventive method preparation up to 0.1～1% (Wt), it can directly be developed as food or lycopene health-care food, and, there is not the dissolvent residual problem in this bitter melon seed aril lycopene powder, production cost is low.
The specific embodiment
The present invention will be described in more detail below in conjunction with embodiment, and the following examples can make those skilled in the art more fully understand the present invention, but do not limit the present invention in any way.
Embodiment 1
Take by weighing 12.1kg and take off the bright aril of seed, under 20 ℃ through spontaneous fermentation in 25 hours; Subsequently, add the 1.5kg tapioca, fully stir, through the colloid mill homogeneous; Equal pledge is carried out filter-press dehydration, under 58 ℃, carry out vacuum drying; Be crushed to granularity and be about 65 orders, obtain bitter melon seed lycopene powder 2.3kg.After testing, the content of lycopene is 0.178% (wt) in the bitter melon seed aril lycopene powder.
Embodiment 2
Take by weighing 12.1kg and take off the bright aril of seed, under 28 ℃ through spontaneous fermentation in 30 hours; Subsequently, add the 1.0kg tapioca, fully stir, through the colloid mill homogeneous; Equal pledge is carried out filter-press dehydration, under 56 ℃, carry out vacuum drying; Be crushed to granularity and be about 65 orders, obtain bitter melon seed lycopene powder 1.8kg.After testing, the content of lycopene is 0.250% (wt) in the bitter melon seed aril lycopene powder.
Embodiment 3
Take by weighing 12.1kg and take off the bright aril of seed, under 32 ℃ through spontaneous fermentation in 24 hours; Subsequently, add the 0.3kg tapioca, fully stir, through the colloid mill homogeneous; Equal pledge is carried out filter-press dehydration, under 58 ℃, carry out vacuum drying; Be crushed to granularity and be about 65 orders, obtain bitter melon seed lycopene powder 1.2kg.After testing, the content of lycopene is 0.351% (wt) in the bitter melon seed aril lycopene powder.
Embodiment 4
Take by weighing 12.1kg and take off the bright aril of seed, under 38 ℃ through spontaneous fermentation in 10 hours; Subsequently, add the 0.7kg tapioca, fully stir, through the colloid mill homogeneous; Equal pledge is carried out filter-press dehydration, under 58 ℃, carry out vacuum drying; Be crushed to granularity and be about 65 orders, obtain bitter melon seed lycopene powder 2.2kg.After testing, the content of lycopene is 0.185% (wt) in the bitter melon seed aril lycopene powder.
Embodiment 5
Take by weighing 12.1kg and take off the bright aril of seed, under 40 ℃ through spontaneous fermentation in 65 hours; Subsequently, add the 1.0kg tapioca, fully stir, through the colloid mill homogeneous; Equal pledge is carried out filter-press dehydration, under 58 ℃, carry out vacuum drying; Be crushed to granularity and be about 65 orders, obtain bitter melon seed lycopene powder 2.0kg.After testing, the content of lycopene is 0.198% (wt) in the bitter melon seed aril lycopene powder.
Embodiment 6
Take by weighing 12.1kg and take off the bright aril of seed, at 28 ℃, pH value is under 6 conditions, adds 75g saccharomyces formosensis bacterium No. 396 (F-396), through 25 hours anaerobic fermentations; Subsequently, add the 0.8kg tapioca, fully stir, through the colloid mill homogeneous; Equal pledge is carried out filter-press dehydration, under 58 ℃, carry out vacuum drying; Be crushed to granularity and be about 65 orders, obtain bitter melon seed lycopene powder 1.6kg.After testing, the content of lycopene is 0.249% (wt) in the bitter melon seed aril lycopene powder.
Embodiment 7
Take by weighing 12.1kg and take off the bright aril of seed, at 32 ℃, pH value is under 3 conditions, adds 75g saccharomyces formosensis bacterium No. 396 (F-396), through 30 hours anaerobic fermentations; Subsequently, add the 1.4kg tapioca, fully stir, through the colloid mill homogeneous; Equal pledge is carried out filter-press dehydration, under 56 ℃, carry out vacuum drying; Be crushed to granularity and be about 65 orders, obtain bitter melon seed lycopene powder 1.9kg.After testing, the content of lycopene is 0.225% (wt) in the bitter melon seed aril lycopene powder.
Embodiment 8
Take by weighing 12.1kg and take off the bright aril of seed, at 38 ℃, pH value is under 4 conditions, adds 75g saccharomyces formosensis bacterium No. 396 (F-396), through 60 hours anaerobic fermentations; Subsequently, add the 1.0kg tapioca, fully stir, through the colloid mill homogeneous; Equal pledge is carried out filter-press dehydration, under 58 ℃, carry out vacuum drying; Be crushed to granularity and be about 65 orders, obtain bitter melon seed lycopene powder 2.0kg.After testing, the content of lycopene is 0.195% (wt) in the bitter melon seed aril lycopene powder.
Detect test
The detection step of the tomato content in the foregoing description comprises:
(1) drafting of calibration curve
Take by weighing 0.025g the Sudan I pigment, use a small amount of anhydrous alcohol solution.Move in the 50ml volumetric flask, and be diluted to scale, shake up with absolute ethyl alcohol.
Accurately draw above-mentioned standard liquid 0.26,0.52,0.78,1.04,1.30ml, inject one group of 50ml volumetric flask respectively, be diluted to scale with absolute ethyl alcohol, obtain being equivalent to the lycopene standard liquid of 0.5,1.0,1.5,2.0,2.5 μ g/ml after shaking up.Then, standard liquid being injected the 1cm cuvette respectively, under the maximum absorption wavelength of lycopene (about 485nm), is blank solution with distilled water, measures absorbance respectively.With the absorbance that records is ordinate, the Sudan I pigment standard liquid suitable lycopene concentration be abscissa, the drawing standard curve.
(2) extraction of lycopene in the sample
A, take by weighing the 1g sample, wrap with filter paper.
B, the diethyl ether solution that contains 2% carrene with 80ml carry out Soxhlet extract sample (40 ℃ of water-baths, 3h).Then, extract is quantitatively moved in the volumetric flask of 100ml.
C, draw the 1ml extract with dilution with toluene to debita spissitudo.
(3) measure
Above-mentioned dilution is moved in the 1ml cuvette, under the maximum absorption wavelength of lycopene (about 485nm), is blank solution with distilled water, uses the spectrophotometric determination absorbance, draws the concentration of lycopene from calibration curve.
(4) analysis result calculates
Content of lycopene such as following table that the bitter melon seed aril lycopene powder that embodiment 1～8 is obtained obtains by above-mentioned detection test:
From above-mentioned table as can be seen, [every 100g is ripe, and tomato contains 3～14mg lycopene to content of lycopene in the bitter melon seed aril lycopene powder that obtains by the present invention approximately with the content of lycopene in the ripe tomato, i.e. 0.003～0.014% (Wt)], and the content of lycopene in the ripe bitter melon seed aril [the bitter melon seed aril of every 100g maturation contains 20～60mg lycopene approximately, i.e. 0.020～0.060% (Wt)] compare, be significantly improved.
Claims (8)
1. the preparation method of a bitter melon seed aril lycopene powder, it comprises the steps:
(1) get ripe bright bitter melon seed aril and carry out spontaneous fermentation at 20～45 ℃, fermentation time is 8～72 hours;
(2) with the starch based auxiliary material: aril=1: 70～1: 7 (Wt) ratio adds the abundant stirring and evenly mixing of starch based auxiliary material, homogeneous;
(3) carry out filter-press dehydration, drying;
(4) pulverizing obtains bitter melon seed aril lycopene powder.
2. preparation method according to claim 1 is characterized in that described fermentation temperature is 30～35 ℃.
3. preparation method according to claim 1 is characterized in that described fermentation time is 23～25 hours.
4. preparation method according to claim 1 is characterized in that described starch based auxiliary material is 1: 60～1: 40 with the aril mass ratio.
5. the preparation method of a bitter melon seed aril lycopene powder, it comprises the steps:
(1) get ripe bright bitter melon seed aril and add saccharomycete carry out anaerobic fermentation under 20～45 ℃, PH＜7 conditions, fermentation time is 8～72 hours;
(2) with the starch based auxiliary material: aril=1: 70～1: 7 (Wt) ratio adds the abundant stirring and evenly mixing of starch based auxiliary material, homogeneous;
(3) carry out filter-press dehydration, drying;
(4) pulverizing obtains bitter melon seed aril lycopene powder.
6. preparation method according to claim 5 is characterized in that described fermentation temperature is 30～35 ℃.
7. preparation method according to claim 5 is characterized in that described fermentation time is 23～25 hours.
8. preparation method according to claim 5 is characterized in that described starch based auxiliary material is 1: 60～1: 40 with the aril mass ratio.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2004100006451A CN1285281C (en) | 2004-01-15 | 2004-01-15 | Preparation of bitter gourd seed aril lycopene powder |
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| CNB2004100006451A CN1285281C (en) | 2004-01-15 | 2004-01-15 | Preparation of bitter gourd seed aril lycopene powder |
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| CN1640313A CN1640313A (en) | 2005-07-20 |
| CN1285281C true CN1285281C (en) | 2006-11-22 |
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