CN1283262C - Dry scorpion venom powder and its preparation - Google Patents
Dry scorpion venom powder and its preparation Download PDFInfo
- Publication number
- CN1283262C CN1283262C CNB2003101050071A CN200310105007A CN1283262C CN 1283262 C CN1283262 C CN 1283262C CN B2003101050071 A CNB2003101050071 A CN B2003101050071A CN 200310105007 A CN200310105007 A CN 200310105007A CN 1283262 C CN1283262 C CN 1283262C
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- China
- Prior art keywords
- venom
- scorpion venom
- powder
- scorpio
- scorpions
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
The present invention relates to dry scorpion venom powder and a preparing method thereof. Firstly, healthy scorpions with good quality are selected and then preheated at 25 to 35 DEG C, and before extraction, physiological saline is used for moistening. Secondly, the scorpions are arranged on venom extracting devices in order in an aseptic constant temperature room, electricity is supplied to make the scorpions discharge venom, and the venom is collected and then treated. Finally, post-treatment is carried out in an aseptic room, crystals in the shape of a needle cylinder are formed and then are tested, and after split charging in vacuum according to bottom/gram, the crystals are lyophilized. The dry scorpion venom powder is refrigerated at the constant temperature of-5 DEG C for spare use and can be stored for a long term. The present invention provides dry scorpion venom powder with the advantages of extraction of pure original venom, large extraction quantity and long-stem storage and also provides a method for preparing the dry scorpion venom powder.
Description
Technical field: the preparation method that the present invention relates to a kind of scorpion venom dry powder.
Background technology: at present, scorpion venom is mainly used in biological preparation and the bio-pharmaceuticals, or carry out deep processing as raw material and batching etc., and obtain pharmaceutical formulation and clinical practice, especially effect is remarkable in the treatment difficult miscellaneous diseases, because being widely used of scorpion venom, consumption is big, is that the present scorpion venom extractive technique of China can't satisfy, in addition, the venom that extracts exists with liquid form mostly at present, can not long preservation.
Summary of the invention: in order to solve the problem of above-mentioned existence, the invention provides that the former poison of a kind of extraction is pure, extracted amount is big, and the preparation method of scorpion venom dry powder that can long preservation.
Concrete technical scheme is as follows: a kind of preparation method of scorpion venom dry powder, and it may further comprise the steps:
1. screening: screen healthy fine Scorpio, preheating under 25 ℃-35 ℃ condition is used the normal saline dewing before extracting;
2. extract: be extracted in that sterile constant-temperature is indoor to carry out, Scorpio be emitted on to carry in order to give electricity on the malicious equipment, make Scorpio discharge venom, handle after the collection.
3. post processing: post processing is carried out in sterilizing room, forms pin column crystallization body and tests, by lyophilizing after the packing of every bottle/gram vacuum;
4. cold preservation: it is standby to carry out constant temperature cold preservation under-5 ℃, can long preservation.
The present invention has the following advantages:
1, adopt the micro-electrical stimulation means under aseptic condition, make its toxin expelling, the poison amount is big, and former poison is pure.
2, can long preservation.
3, contain rich in amino acid, see following table for details:
| Amino acid analysis | ||
| Project | Unit | The result |
| Aspartic acid ASP | mg/100g | 4649 |
| Threonine THR | mg/100g | 1527 |
| Serine SER | mg/100g | 980 |
| Glutamic acid GLU | mg/100g | 2045 |
| Glycine GLY | mg/100g | 1907 |
| Alanine ALA | mg/100g | 1487 |
| Cystine CYS | mg/100g | 666 |
| Valine VAL | mg/100g | 2374 |
| Methionine MET | mg/100g | 415 |
| Isoleucine ILE | mg/100g | 890 |
| Leucine LEU | mg/100g | 1188 |
| Tyrosine TYR | Mg/100g | 1740 |
| Phenylalanine PHE | mg/100g | 1047 |
| Lysine LYS | mg/100g | 2960 |
| Ammonia NH3 | mg/100g | 175 |
| Histidine HIS | mg/100g | 511 |
| Arginine ARG | mg/100g | 1821 |
| Proline PRO | mg/100g | 1230 |
| Total amino acid content | mg/100g | 27612 |
The specific embodiment:
Embodiment: a kind of scorpion venom dry powder and preparation method thereof, it may further comprise the steps:
1, screening: screen healthy fine Scorpio, preheating under 25 ℃-35 ℃ condition is used the normal saline dewing before extracting;
2, extract: be extracted in that sterile constant-temperature is indoor to carry out, Scorpio be emitted on to carry in order to give electricity on the malicious equipment, make Scorpio discharge venom, handle after the collection.
3, post processing: post processing is carried out in sterilizing room, forms pin column crystallization body and tests, by lyophilizing after the packing of every bottle/gram vacuum;
4, cold preservation: it is standby to carry out constant temperature cold preservation under-5 ℃, can long preservation.
Claims (1)
1, a kind of preparation method of scorpion venom dry powder is characterized in that it may further comprise the steps:
1. screening: screen healthy fine Scorpio, preheating under 25 ℃-35 ℃ condition is used the normal saline dewing before extracting;
2. extract: be extracted in that sterile constant-temperature is indoor to carry out, Scorpio be emitted on to carry in order to give electricity on the malicious equipment, make Scorpio discharge venom, handle after the collection;
3. post processing: post processing is carried out in sterilizing room, forms pin column crystallization body and tests, by lyophilizing after the packing of every bottle/gram vacuum;
4. cold preservation: it is standby to carry out constant temperature cold preservation under-5 ℃, can long preservation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101050071A CN1283262C (en) | 2003-11-05 | 2003-11-05 | Dry scorpion venom powder and its preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101050071A CN1283262C (en) | 2003-11-05 | 2003-11-05 | Dry scorpion venom powder and its preparation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1613454A CN1613454A (en) | 2005-05-11 |
| CN1283262C true CN1283262C (en) | 2006-11-08 |
Family
ID=34757154
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2003101050071A Expired - Fee Related CN1283262C (en) | 2003-11-05 | 2003-11-05 | Dry scorpion venom powder and its preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1283262C (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CZ2013840A3 (en) * | 2013-11-01 | 2015-01-28 | Příhoda S.R.O. | Air-conditioning system part |
-
2003
- 2003-11-05 CN CNB2003101050071A patent/CN1283262C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1613454A (en) | 2005-05-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20061108 Termination date: 20101105 |