CN1282729C - Technique for producing biologic diesel oil through combination of different lipases - Google Patents
Technique for producing biologic diesel oil through combination of different lipases Download PDFInfo
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- CN1282729C CN1282729C CNB200510011623XA CN200510011623A CN1282729C CN 1282729 C CN1282729 C CN 1282729C CN B200510011623X A CNB200510011623X A CN B200510011623XA CN 200510011623 A CN200510011623 A CN 200510011623A CN 1282729 C CN1282729 C CN 1282729C
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P30/00—Technologies relating to oil refining and petrochemical industry
- Y02P30/20—Technologies relating to oil refining and petrochemical industry using bio-feedstock
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Abstract
The present invention provides a technology for producing biologic diesel oil by combining different lipase, which belongs to the technical field of biologic grease synthesis. Two kinds of lipase with different catalytic performance or different lipase with different catalytic performance are combined to be used; especially, the present invention relates to a new technology in which immobilized lipase with 1, 3 position specificity is combined with immobilized lipase without position specificity to be used; grease raw materials are effectively converted to generate biologic diesel oil by the catalytic characteristics of the different lipase. The present invention is characterized in that lipase with different performance is used by mixing or by separating ' connection in series '; the present invention is used for catalyzing grease raw materials to react with short chain alcohol, such as methanol, etc. or other acyl group acceptors to prepare the biologic diesel oil; the reaction can be carried out at intervals in a batch or carried out continuously; reproducible grease raw materials can be effectively converted in a short time by the combination use of the different lipase; therefore, the biologic diesel oil with high yield is obtained.
Description
Technical field
The invention belongs to the bio-oil synthesis technical field, particularly a kind of technology of producing biologic diesel oil through combination of different lipases is used in combination the lipase of different catalytic performances, is used for the novel process that the catalysis of renewable glyceride stock transforms biodiesel synthesis.
Background technology
By the long chain fatty acid ester class material that transesterification reaction generates, is a kind of novel pollution-free renewable energy source by the bio-oil raw material, is called as biofuel.Its combustionproperty can be that diesel oil matches in excellence or beauty with traditional oil, and research of biofuel at present and application have been subjected to widely to be paid close attention to.
Biofuel mainly is to use chemical method production at present, promptly uses animal-plant oil and some low-carbon alcohol (methyl alcohol or ethanol) to carry out transesterification reaction under alkali or an acidic catalyst effect, generates corresponding fatty acid methyl ester or ethyl ester.Chemical method prepares biofuel and has some unavoidable problem, and discharging causes secondary pollution etc. to environment easily as spent acid (alkali) liquid.Because free fatty acids and the less water of utilizing the biological enzyme biodiesel synthesis to have in reaction conditions gentleness, non-pollutant discharge, the glyceride stock do not influence advantages such as enzymatic reaction, meet the developing direction of Green Chemistry, thereby are subject to people's attention day by day.But also there are some problem demanding prompt solutions in enzyme process biodiesel synthesis technology, and is too high as the cost of enzyme, and enzymatic conversion method glyceride stock biodiesel synthesis yield is relatively low etc.So the biological enzyme technology that exploitation is cheap, the yield that reduces biological enzyme use cost and raising biological enzyme biodiesel synthesis is to realize the key of biological enzyme industrialization production biofuel.
The biological enzyme glyceride stock of being reported at present that utilizes carries out in the building-up process of biofuel, generally all is to use the single fat enzyme to carry out the conversion of glyceride stock.Utilize this single enzyme method technique to carry out the synthetic of biofuel, the yield of general biofuel is relatively low.
Summary of the invention
The objective of the invention is to propose a kind of technology of producing biologic diesel oil through combination of different lipases; utilize the catalysis characteristics of different lipase; especially the substrate specificity of lipase and position specific; two or more lipase are used in combination; be used for the catalyzed oil fat raw material with short chain alcohol such as methyl alcohol and other acyl acceptor biodiesel synthesis that reacts; thereby on the basis that obtains higher bio-diesel yield, effectively reduce the biological enzyme cost.
The 2-5 kind lipase that the present invention is different with catalytic performance is used in combination, and utilizes the catalysis characteristics of different lipase, and glyceride stock is effectively transformed the generation biofuel.It is characterized in that:
The lipase that performance is different mixes use or separately " polyphone " use, especially will have 1, and the lipase of 3-position specific is used in combination with the lipase that does not have position specific; Be used for the reaction of catalyzed oil fat raw material and short chain alcohol and carry out the preparation of biofuel.The reaction mol ratio of short chain alcohol and glyceride stock is 1: 1-5: 1, and be reflected in the biochemical reactor that is suitable for enzymic catalytic reaction and carry out, temperature of reaction is 30 ℃-50 ℃; Reaction can be intermittently batch be carried out or is carried out continuously, and reaction system is a solvent-free system, contains the system or the organic reaction medium system of organic solvent, and organic solvent comprises the trimethyl carbinol, methyl acetate, ethyl acetate, hexane or acetone.Reaction process can have water absorber or not have water absorber.This novel process can almost completely transform the generation biofuel with glyceride stock fast under the situation of using less enzyme amount.
Lipase of the present invention is microbial lipase, preferentially comprises immobilized lipase Lipozyme TL (deriving from Thermomyces lanuginosus), Lipozyme RM (deriving from Rhizomucor miehe).Novozym435 (deriving from Candida antarctic).The immobilized lipase that derives from Candida antarctic comprises Novozym435, the immobilized lipase that derives from Thermomyces lanuginosus comprises Lipozyme TL, and the immobilized lipase that derives from Rhizomucor miehei is drawn together Lipozyme RM.
Grease of the present invention is that bio-oil comprises Vegetable oil lipoprotein, animal grease, waste edible oil or oil refining pin material.
Vegetable oil lipoprotein of the present invention is Viscotrol C, rapeseed oil, soybean oil, peanut oil, Semen Maydis oil, Oleum Gossypii semen, Rice pollard oil or algae grease.
Animal grease of the present invention is fish oil, lard.
Short chain alcohol of the present invention is methyl alcohol, ethanol, propyl alcohol, butanols or amylalcohol.
Other acyl acceptor of the present invention is short-chain aliphatic ester RCOOR ', and wherein R and R ' are for having the alkyl of 1-5 carbon atom.
" polyphone " of the present invention uses and is meant that reactant transforms through lipase 1 earlier, transforms or lipase 3 conversions through lipase 2 again.
The present invention is that the lipase with different catalytic performances effectively is used in combination, be used to transform the preparation that the renewable oils fat raw material carries out biofuel, its beneficial effect is when using less enzyme amount (also promptly effectively reducing the biological enzyme cost), can obtain the biofuel of high yield.
Embodiment
Further specify the present invention below by embodiment, but the present invention is not limited only to following examples:
Embodiment 1
With mol ratio 4: 1 methyl alcohol and rapeseed oil, and based on the trimethyl carbinol of grease volume 50%, pack into and mix in the tool plug triangular flask, and place can be temperature automatically controlled reciprocal shaking table be heated to 40 ℃ after, the immobilized lipase Lipozyme TL that adds oil quality 2% begins reaction, reacted 5 hours, bio-diesel yield is about 70%.Reaction mixture is taken out, utilize then another kind of immobilized lipase Novozym 435 (based on initial oil quality 0.5%) continue to transform 4 hours, bio-diesel yield is about 100%.
Embodiment 2
With mol ratio is 3.5: 1 ethanol and Oleum Gossypii semen, and based on the trimethyl carbinol of grease volume 20%, in the enzyme reactor of packing into, 35 ℃ of chuck temperature controls, the immobilized lipase Lipozyme RM that adds oil quality 1% begins reaction.Reacted 4 hours, bio-diesel yield is about 65%; Reaction mixture flow into another be equipped with immobilized lipase Novozym 435 (based on initial oil quality 0.5%) enzyme reactor continue to transform 5 hours, bio-diesel yield is about 100%.
Embodiment 3
With mol ratio 3.5: 1 methyl alcohol and oleic acid, pack into and mix in the tool plug triangular flask, and place can be temperature automatically controlled reciprocal shaking table be heated to 40 ℃ after, the immobilized lipase Lipozyme TL that adds oil quality 1% begins reaction, reacted 2 hours, bio-diesel yield is about 65%, then reaction mixture is taken out, handle back (adding 3 molecular sieve or silica gel) with the suction of water-retaining agents such as molecular sieve or silica gel based on oleic acid 2%, utilize then immobilized lipase Novozym 435 (based on initial oil quality 0.2%) continue to transform 5 hours, bio-diesel yield is about 100%.
Embodiment 4
Be 3.5: 1 methyl alcohol and waste oil with mol ratio and mix based on the trimethyl carbinol of waste oil quality 10%, place the immobilized lipase Lipozyme TL that is equipped with based on oil quality 1% to begin reaction (35 ℃ of chuck temperature controls), reacted 2 hours, bio-diesel yield is about 70%; After reaction mixture flows into another suction post that water-retaining agents such as molecular sieve or silica gel are housed suction processing, flow into then be equipped with immobilized lipase Novozym 435 (based on initial oil quality 0.5%) continue to transform 3 hours (35 ℃ of chuck temperature controls), bio-diesel yield is about 100%.
Embodiment 5
With mol ratio 5: 1 ethanol and rapeseed oil, and based on the trimethyl carbinol of grease volume 100%, pack into and mix in the tool plug triangular flask, and place can be temperature automatically controlled reciprocal shaking table be heated to 40 ℃ after, add the immobilized lipase Lipozyme TL of oil quality 2% and based on 0.5% immobilized lipase Novozym435 reaction of oil quality 5 hours, bio-diesel yield was about 100%.
Embodiment 6
With mol ratio 5: 1 methyl alcohol and Oleum Gossypii semen, and based on the methyl acetate of grease volume 80%, pack into and mix in the tool plug triangular flask, and place can be temperature automatically controlled reciprocal shaking table be heated to 40 ℃ after, add the immobilized lipase Lipozyme TL of oil quality 3% and based on 1% immobilized lipase Novozym435 reaction of oil quality 4 hours, bio-diesel yield was about 100%.
Embodiment 7
With mol ratio 4: 1 methyl alcohol and soybean oil, and based on the acetone of grease volume 80%, pack into and mix in the tool plug triangular flask, and place can be temperature automatically controlled reciprocal shaking table be heated to 40 ℃ after, add the immobilized lipase Lipozyme TL of oil quality 5% and based on 0.2% immobilized lipase Novozym 435 reactions of oil quality 5 hours, bio-diesel yield was about 100%.
Embodiment 8
Be 3.5: 1 methyl alcohol and Oleum Gossypii semen with mol ratio and mix based on the trimethyl carbinol of oil quality 50%, place the immobilized lipase Lipozyme TL that is equipped with based on oil quality 1% to begin reaction (35 ℃ of chuck temperature controls), reacted 2 hours, bio-diesel yield is about 70%; After reaction mixture flows into another suction post that water-retaining agents such as molecular sieve or silica gel are housed suction processing, flow into then be equipped with immobilized lipase Novozym 435 (based on initial oil quality 0.5%) continue to transform 3 hours (35 ℃ of chuck temperature controls), bio-diesel yield is about 100%.
Embodiment 9
Be 3.5: 1 methyl alcohol and plam oil with mol ratio and mix based on the trimethyl carbinol of oil quality 100%, place the immobilized lipase Lipozyme TL that is equipped with based on oil quality 1% to begin reaction (35 ℃ of chuck temperature controls), reacted 3 hours, bio-diesel yield is about 70%; After reaction mixture flows into another suction post that water-retaining agents such as molecular sieve or silica gel are housed suction processing, flow into then be equipped with immobilized lipase Novozym 435 (based on initial oil quality 0.5%) continue to transform 4 hours (35 ℃ of chuck temperature controls), bio-diesel yield is about 100%.
Embodiment 10
Be 5: 1 methyl alcohol and fish oil with mol ratio and mix based on the trimethyl carbinol of oil quality 150%, place the immobilized lipase Lipozyme TL that is equipped with based on oil quality 1% to begin reaction (45 ℃ of chuck temperature controls), reacted 5 hours, bio-diesel yield is about 70%; After reaction mixture flows into another suction post that water-retaining agents such as molecular sieve or silica gel are housed suction processing, flow into then be equipped with immobilized lipase Novozym 435 (based on initial oil quality 0.5%) the anti-device of enzyme continue to transform 3 hours (45 ℃ of chuck temperature controls), bio-diesel yield is about 100%.
Embodiment 11
Be 4: 1 methyl alcohol and " sewer oil " with mol ratio and mix based on the trimethyl carbinol of oil quality 20%, with 70 liters/speed Continuous Flow hourly through the enzyme reactor of immobilized lipase Lipozyme TL (based on oil quality 1%) is housed, the suction post suction that molecular sieve or silica gel are housed of then flowing through is handled, flow through again enzyme reactor that immobilized lipase Novozym 435 is housed (based on initial oil quality 0.5%) (35 ℃ of chuck temperature controls), bio-diesel yield is about 100%.
Embodiment 12
Be 4.5: 1 ethanol and " sewer oil " with mol ratio and mix based on the trimethyl carbinol of oil quality 20%, with the enzyme reactor (chuck temperature control 35 ℃) of 50 liters/speed Continuous Flow hourly through immobilized lipase Lipozyme TL (based on oil quality 1%) is housed, the suction post suction that molecular sieve or silica gel are housed of then flowing through is handled, flow through again enzyme reactor that immobilized lipase Novozym 435 is housed (based on initial oil quality 0.5%) (35 ℃ of chuck temperature controls), bio-diesel yield is about 100%.
Embodiment 13
Be 5: 1 methyl alcohol and lard with mol ratio and mix based on the trimethyl carbinol of oil quality 200%, with the enzyme reactor (chuck temperature control 50 ℃) of 100 liters/speed Continuous Flow hourly through immobilized lipase Lipozyme TL (based on oil quality 1%) is housed, the suction post suction that molecular sieve or silica gel are housed of then flowing through is handled, flow through again enzyme reactor that immobilized lipase Novozym 435 is housed (based on initial oil quality 0.5%) (50 ℃ of chuck temperature controls), bio-diesel yield is about 100%.
Embodiment 14
Be 5: 1 methyl alcohol and rapeseed oil with mol ratio and mix based on the ethyl acetate of oil quality 200%, with the enzyme reactor (chuck temperature control 30 ℃) of 200 liters/speed Continuous Flow hourly through immobilized lipase Lipozyme TL (based on oil quality 2%) is housed, the suction post suction that molecular sieve or silica gel are housed of then flowing through is handled, flow through again enzyme reactor that immobilized lipase Novozym 435 is housed (based on initial oil quality 1%) (30 ℃ of chuck temperature controls), bio-diesel yield is about 100%.
Embodiment 15
Be 4: 1 the pure and mild Rice pollard oil of ethanol with mol ratio and mix based on the methyl acetate of oil quality 100%, with the enzyme reactor (chuck temperature control 35 ℃) of 150 liters/speed Continuous Flow hourly through immobilized lipase Lipozyme TL (based on oil quality 3%) is housed, the suction post suction that molecular sieve or silica gel are housed of then flowing through is handled, flow through again enzyme reactor that immobilized lipase Novozym 435 is housed (based on initial oil quality 0.2%) (35 ℃ of chuck temperature controls), bio-diesel yield is about 100%.
Embodiment 16
With mol ratio is that 5: 1 methyl alcohol and " swill oil " mix, with the enzyme reactor (chuck temperature control 35 ℃) of 250 liters/speed Continuous Flow hourly through immobilized lipase Lipozyme TL (based on oil quality 0.5%) is housed, the suction post suction that molecular sieve or silica gel are housed of then flowing through is handled, the enzyme reactor (35 ℃ of chuck temperature controls) of flowing through again immobilized lipase Lipozyme TL (based on oil quality 0.5%) being housed, the suction post suction that molecular sieve or silica gel are housed of then flowing through is handled, and then the enzyme reactor that immobilized enzyme Novozym 435 is housed of flowing through (based on initial oil quality 1%) (35 ℃ of chuck temperature controls), bio-diesel yield is about 100%.
Embodiment 17
With mol ratio is that 4: 1 methyl alcohol and " swill oil " mix, with the enzyme reactor (chuck temperature control 35 ℃) of 200 liters/speed Continuous Flow hourly through immobilized lipase Lipozyme TL (based on oil quality 0.5%) is housed, the suction post suction that molecular sieve or silica gel are housed of then flowing through is handled, the enzyme reactor (35 ℃ of chuck temperature controls) of flowing through again immobilized lipase Lipozyme RM (based on oil quality 0.5%) being housed, the suction post suction that molecular sieve or silica gel are housed of then flowing through is handled, and then the enzyme reactor that immobilized enzyme Novozym 435 is housed of flowing through (based on initial oil quality 1%) (35 ℃ of chuck temperature controls), bio-diesel yield is about 100%.
Embodiment 18
Be 6: 1 methyl alcohol and lard with mol ratio and mix based on the hexane of grease 50%, with the enzyme reactor (chuck temperature control 45 ℃) of 150 liters/speed Continuous Flow hourly through immobilized lipase Lipozyme RM (based on oil quality 0.5%) is housed, the suction post suction that molecular sieve or silica gel are housed of then flowing through is handled, the enzyme reactor (45 ℃ of chuck temperature controls) of flowing through again immobilized lipase Lipozyme TL (based on oil quality 0.5%) being housed, the suction post suction that molecular sieve or silica gel are housed of then flowing through is handled, and then the enzyme reactor that immobilized enzyme Novozym435 is housed of flowing through (based on initial oil quality 1%) (45 ℃ of chuck temperature controls), bio-diesel yield is about 100%.
Claims (7)
1. the technology of a producing biologic diesel oil through combination of different lipases, the 2-5 kind lipase that catalytic performance is different are used in combination, and utilize the catalysis characteristics of different lipase, and glyceride stock is effectively transformed the generation biofuel; It is characterized in that: the lipase that performance is different mixes use, be used for the reaction of catalyzed oil fat raw material and short chain alcohol and carry out the preparation of biofuel, the reaction mol ratio of short chain alcohol and glyceride stock is 1: 1-5: 1, be reflected in the biochemical reactor that is suitable for enzymic catalytic reaction and carry out, temperature of reaction is 30 ℃-50 ℃; Reaction intermittently batch is carried out or is carried out continuously, reaction system is a solvent-free system, the system that contains organic solvent, or organic reaction medium system, reaction process has water absorber or does not have water absorber, each enzyme amount of using is based on the heavy 0.2%-5% of oil, glyceride stock is transformed generate biofuel fast; The lipase that described performance is different is meant to have 1, and the immobilized lipase of 3-position specific is used in combination with the immobilized lipase that does not have position specific; Described organic solvent comprises the trimethyl carbinol, methyl acetate, ethyl acetate, hexane or acetone.
2. according to the described technology of claim 1, it is characterized in that: described lipase is microbial lipase, comprises immobilized lipase; Described immobilized lipase comprises and derives from Candida antarctic, the lipase of Thermomyceslanuginosus and Rhizomucor miehei; The immobilized lipase that derives from Candida antarctic comprises Novozym435, the immobilized lipase that derives from Thermomyces lanuginosus comprises LipozymeTL, and the immobilized lipase that derives from Rhizomucor miehei comprises Lipozyme RM.
3. according to the described technology of claim 1, it is characterized in that: water absorber comprises silica gel, the molecular sieve water-retaining agent; The molecular sieve water-retaining agent comprises 3 molecular sieves.
4. according to the described technology of claim 1, it is characterized in that: described grease is a bio-oil, comprises Vegetable oil lipoprotein, animal grease, waste edible oil or oil refining pin material.
5. according to claim 1 or 4 described technologies, it is characterized in that: described Vegetable oil lipoprotein is Viscotrol C, rapeseed oil, soybean oil, peanut oil, Semen Maydis oil, Oleum Gossypii semen, Rice pollard oil or algae grease.
6. according to claim 1 or 4 described technologies, it is characterized in that: described animal grease is fish oil or lard.
7. according to the described technology of claim 1, it is characterized in that: R is the alkyl with 1-5 carbon atom in the described short chain alcohol roh, comprises methyl alcohol, ethanol, propyl alcohol, butanols or amylalcohol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200510011623XA CN1282729C (en) | 2005-04-25 | 2005-04-25 | Technique for producing biologic diesel oil through combination of different lipases |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200510011623XA CN1282729C (en) | 2005-04-25 | 2005-04-25 | Technique for producing biologic diesel oil through combination of different lipases |
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| CN1687313A CN1687313A (en) | 2005-10-26 |
| CN1282729C true CN1282729C (en) | 2006-11-01 |
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| CNB200510011623XA Expired - Fee Related CN1282729C (en) | 2005-04-25 | 2005-04-25 | Technique for producing biologic diesel oil through combination of different lipases |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108342216A (en) * | 2018-02-22 | 2018-07-31 | 徐州市恒源生物油脂科技有限公司 | A kind of biodiesel |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101235351B (en) * | 2008-02-27 | 2012-07-18 | 浙江大学 | Fixed enzyme membrane reactor, preparation thereof and method for producing biological diesel oil by the same |
| CN101412921B (en) * | 2008-07-04 | 2012-12-12 | 苏悟敏 | Formula and preparation of biocatalyst for modification and improvement of petroleum fuel oil |
| CN102021207B (en) * | 2010-11-04 | 2013-08-14 | 清华大学 | On-line dehydration process for preparing biodiesel from renewable grease through catalysis of lipase |
| CN102676304B (en) * | 2011-03-15 | 2013-06-19 | 清华大学 | Preparation method for biodiesel fuel |
| CN103710158B (en) * | 2014-01-02 | 2015-01-21 | 华南农业大学 | Method for producing biodiesel by using kitchen wastes |
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2005
- 2005-04-25 CN CNB200510011623XA patent/CN1282729C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108342216A (en) * | 2018-02-22 | 2018-07-31 | 徐州市恒源生物油脂科技有限公司 | A kind of biodiesel |
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