CN1281689A - Vaginal spongy suppository for iducing ewe estrus - Google Patents
Vaginal spongy suppository for iducing ewe estrus Download PDFInfo
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- CN1281689A CN1281689A CN 00112505 CN00112505A CN1281689A CN 1281689 A CN1281689 A CN 1281689A CN 00112505 CN00112505 CN 00112505 CN 00112505 A CN00112505 A CN 00112505A CN 1281689 A CN1281689 A CN 1281689A
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- ewe
- sponge
- progesterone
- oestrus
- intravaginal
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Abstract
The present invention relates to a vaginal sponge suppository containing 150-350 mg of progesterone for estrus induction of ewe. Said invented sponge suppository is placed in the vagina of ewe for 10-20 days, ofter the suppository is removed, it can artificially induce ewe in heat in estrus and anestrus season. Said invention is easy to produce, low in cost, has no any side effects, and its inductive estrous rate is up to 80%, and pregnancy rate can be up to 77.2%.
Description
The present invention is a kind of to be used to the intravaginal sponge of inducing ewe to oestrus, and belongs to the medicine bolt, is used for induced animal and oestruses, and improves the animal reproductive performance, improves animal reproduction power.
In sheep and goat is produced, improve the ewe reproductive performance and close important.The artificial induction ewe oestruses, and can shorten the lambing interval, reduces the nonpregnant time of ewe, thereby reduces feeding cost; The artificial induction oestruses, and can in a planned way breed to flock of sheep effectively within a certain period of time, thereby make production measures such as breeding, lambing, lamb raising be convenient to management; In the embryo transfer process, can make donor and receptor synchronization of estrus.
Both at home and abroad at present the artificial induction who the adopts method of oestrusing has: one, injection prostaglandin F
2 αOr its analog is (as Cloprostenol, 15 carboprost methylates
2 αDeng) can make the luteolysis on the ewe ovary, thus induce ewe to oestrus; Two, subdermal implantation Progesterone and analog thereof, removing medicine behind the certain hour can estrus induction; Three, adopt Progesterone and Progesterone analog abroad, make carrier with medical rubber hose or plastic tube, place the ewe intravaginal, removing medicine behind the certain hour can induce ewe to oestrus, commercialization production now, as the Chrono-gest45 vaginal sponge that Australian Intervet company produces, be the flurogestone acetate that adopts 45mg; Four, injection estrogen or its analog are as diethylstilbestrol.Method one needs the biphasic injection prostaglandin F
2 αAnd analog, operate more tired, use cost higher (about 4 yuan/only), and the rate of oestrusing not high (30-80%), especially invalid for the ewe of non-activity corpus luteum on the ovary; Method two, three estrus induction effects are better, but method two need carry out subdermal implantation, and are difficult for taking out, and operate loaded down with trivial details.Method three needs from external import, and price is higher, although adopt the effect that the required medicine of Progesterone analog is few, have stronger inhibition to oestrus, the effect that promotes follicular development is not so good as Progesterone, and first non-return rate is low.Though method four can make ewe oestrus, not ovulation mostly.Progesterone chemistry pregnant steroid-4 alkene-3,20 diketone by name is introduced according to People's Republic of China's veterinary drug allusion quotation (People's Republic of China's veterinary drug allusion quotation, agriculture publishing house, 1990, ones, the 192nd page), and the effect of Progesterone and purposes are the antiabortive effects of tool, are used for prevention of miscarriage.
The objective of the invention is the defective that first non-return rate of existence is low respectively in the prior art, operation is loaded down with trivial details, provide a kind of as the intravaginal sponge of inducing ewe to oestrus, can adsorb medicine to greatest extent, after placing the vagina deep, vaginal mucosa can be absorbed with the medicine that inhibition is oestrused and promoted follicular development, can induce ewe to oestrus.Used medicine is free from side effects to ewe; The effect that estrus induction is all arranged at sexual season or non-sexual season; Easy to use, cheap.
Provided by the present invention a kind of as the intravaginal sponge of inducing ewe to oestrus, it is characterized in that: with the medicine Progesterone of estrus induction, consumption is that 200-500mg adds in the sponge, makes the progesterone intravaginal sponge, places the ewe intravaginal 10-20 days.Wherein Progesterone 200-500mg can be dissolved in 2mL detoxification salad oil, be added in the sponge or take by weighing 200-500mg Progesterone dry powder, directly be added in the sponge.The progesterone intravaginal sponge is the cylindrical, conical of diameter 2-5 centimetre, high 3-4 centimetre, the square or equal volume of long 2-5 centimetre, wide 3-4 centimetre irregularly shaped.Spongy embolus band single line rope.
Intravaginal sponge provided by the present invention places the ewe intravaginal, discharges the medicine Progesterone of estrus induction, removes behind the bolt and promptly can effectively induce ewe to oestrus in 2-6 days.The artificial induction who is applicable to all ewes oestruses.Be sexual season and the non-sexual season that is suitable for sheep action time.Through clinical practice, obtain beyond thought effect.Compared with prior art, advantage of the present invention and good effect are as follows:
1, sponge is easy to cutting processing, so spongy embolus is produced easily; Every sheep processing cost of Cloprostenol method needs 4 yuan of expensess for medicine in the prior art, and the Progesterone price is 1500 yuan/kilogram among the present invention, and the medicine cost of every progesterone intravaginal sponge is lower than 0.5 yuan; Because the sponge mesopore is abundanter, to the high adsorption capacity of medicine, makes the inventive method simple to operate.
2, estrus induction rate of the present invention reaches 80%, and particularly first non-return rate reaches 77.2%, is significantly higher than and uses the level that the progestogen analog induces 50% ewe to oestrus in the prior art.Can improve the breeding management level of flock of sheep, manually shorten the lambing interval of ewe, improve the breeding potential of ewe.
3, along with the prolongation of putting the bolt time, the levels of drugs in the bolt constantly descends, until after removing bolt, the level of Progesterone descends always, this with the estrus cycle in, oestrusing the later stage, with the dissolving of corpus luteum, Progesterone level constantly decline is consistent.Modern physiology of reproduction studies show that, the Progesterone of doses and progressively to descend be that reaching maturity of follicle is necessary, and its mechanism has been simulated the natural estrus cycle, plays the effect that promotes follicular development.
4,, adopt the present invention not find that used medicine is to side effect such as the gestation of ewe, fetal development, next estrus cycles by to bull ewe multiple spot tests such as your goat of 205 the local sheep in Xuzhou, poultry stock breeding center, Suqian, Jiangsu 30 bow waves, your goats of Lishui, Nanjing sheep stud 8 bow waves.Sexual season (spring, autumn) or non-sexual season the effect of estrus induction is all arranged in (summer, winter), can both reach 80%;
5, the medicine in the spongy embolus can be absorbed by vaginal mucosa.If Progesterone by oral, then is difficult for being absorbed by pipe intestinal digesting.The present invention adopts the progesterone intravaginal sponge, the progesterone vaginal sponge can be placed on the ewe intravaginal, and medicine can be absorbed easily by vaginal mucosa.
6, in the existing embryo engineering technology, induce ewe to oestrus with the Cloprostenol method after, can make the ewe luteolysis, thereby induce ewe to oestrus.But because follicular development needs a certain amount of progesterone, so during application Cloprostenol method, first non-return rate is often lower.In embryo engineering, the processing of can not breeding of oestrusing need wait until that second feelings phase just can carry out superovulation (make and produce the ovum that surpasses normal quantity on the ewe ovary).Result of the test shows, the inventive method effect in embryo engineering is good, handle and just can carry out superovulation before oestrusing for the first time in the back, average every ewe produces 10.75 pieces of embryos that can transplant, and the present invention can further simplify the operating procedure of embryo engineering, and working time of embryo transfer was shortened to about 20 days from 43 days;
7, the inventive method can make a group ewe can concentrate in 2-3 days to oestrus (this technology claims estrus synchronization), and the rate of oestrusing reaches 80%, and prior art has only 50%; When estrus synchronization was handled, first non-return rate reached 100%, and prior art is 70%.
All spongy embolus, its manufacturing process is as follows:
Medical sponge is cut into the cylindrical conical or square of specification unanimity, two kinds of manufacture methods are arranged then: one, the bolt line is sewn on the sponge ball along the longitudinal axis, the bolt line length is about 10cm, after Progesterone is dissolved in detoxification salad oil or other solvent, add in the sponge ball with dissolved state, can be made into oiliness progesterone intravaginal sponge, can will make dryness progesterone intravaginal sponge after the solvent evaporates by evaporable solvent.Two, above-mentioned sponge ball is cut in half along transverse axis, a cavity is dug out in the centre, and a certain amount of Progesterone powder is added in the duck eye, and reuse bolt line is sewed up two halves, and knotting, and the bolt line length is about 10cm.
The effect of 1 pair of common goat estrus induction of embodiment:
The Progesterone consumption is 250-350mg in the progesterone vaginal suppository.
1, with bromo geramine or potassium permanganate disinfectant the pudendum of ewe is cleaned;
2, spread penicillin or streptomycin powder on the progesterone intravaginal sponge of making, and live sponge ball, insert the vagina deep of ewe, the bolt line is stayed the outer 2-3cm of vaginal orifice with the vaginal sponge clamp.
3, after 15 days,, withdraw from the progesterone intravaginal sponge by tractive bolt line.
4, remove and adopt behind the bolt ram examination feelings method to determine that ewe oestruses in 2-6 days, can breed.
The progesterone consumption is that 250-350mg is (in this scope, the Progesterone consumption does not need accurate measurement), make oiliness progesterone intravaginal sponge, adopt the inventive method to handle 205 local ewes in the peaceful king of the Ju of Xuzhou City market town, directly the progesterone intravaginal sponge is put into ewe vagina deep, the oestrus-inducking rate of common goat 30-60 days puerperal reached 89.2%; Oestrus-inducking rate 60-90 days puerperal can reach 80.8%, and adopts the Cloprostenol method to handle 210 local goats, and the oestrus-inducking rate in 30-60 days puerperal is 50%; Oestrus-inducking rate 60-90 days puerperal can reach 40%.This shows that the present invention can shorten the lambing interval of ewe effectively, improve the lambing percentage of sheep.
Puerperal 60-90 days puerperal of 30-60 days puerperal is more than 90 days
The feelings of the oestrusing phase
Oestrus
Hair is sent out the rate group hair feelings rate hair feelings rate of oestrusing of being impregnated
(% leads number feelings feelings
Number (%) number (% number (%)
The number number) (%)
) processing 205 37 33 89.2 99 80 80.8 69 52 75.4 80.5 77.2 contrasts 210 80 40 50% 80 32 40 50 24 48 45.7 30
Embodiment 2 induces the application of boer goat estrus synchronization in different seasons:
1, with bromo geramine or potassium permanganate disinfectant the pudendum of ewe is cleaned;
2, spread penicillin or streptomycin powder on the progesterone intravaginal sponge of making, and live sponge ball, insert the vagina deep of ewe, the bolt line is stayed the outer 2-3cm of vaginal orifice with the vaginal sponge clamp.
3, at the 13rd day, intramuscular injection hypophysis follicle stimulating hormone 50 units (adding slow releasing agent).
4, after 15 days,, withdraw from the progesterone intravaginal sponge, the Cloprostenol of intramuscular injection simultaneously 0.2mg by tractive bolt line.5, remove and adopt behind the bolt ram examination feelings method to determine that ewe oestruses in 1-4 days, can breed.
Boer goat is oestrused not obvious, is unfavorable for the breeding management of sheep stud, incurs loss through delay breeding time easily.Adopt the inventive method, choosing your goat of 30 bow waves at poultry stock breeding center, Suqian, Jiangsu uses the 150-250mg Progesterone (in this scope, the Progesterone consumption does not need accurate measurement) make oiliness progesterone intravaginal sponge as stated above, put into the ewe intravaginal 15 days, the follicle stimulating hormone of the 13rd day injection 50 units, removed spongy embolus in 15 days, the method can be induced the boer goat estrus synchronization in sexual season and non-sexual season, and first non-return rate reach 100%, and adopt the Cloprostenol rule not induce ewe to oestrus at the non-sexual season of sheep.
The rate of oestrusing (%, first non-return rate estrus time in season
Number/sum) removes bolt non-sexual season on three days same day to the 93.8% (15/16) 100% (15/15) (%, number/sum) sexual season 85.7% (12/14) 100% (12/12) and remove three days same day to the of bolt
Example 3
The application of the inventive method in embryo engineering:
1, with bromo geramine or potassium permanganate disinfectant the pudendum of ewe is cleaned;
2, spread penicillin or streptomycin powder on the progesterone intravaginal sponge of making, and live sponge ball, insert the vagina deep of ewe, the bolt line is stayed the outer 2-3cm of vaginal orifice with the vaginal sponge clamp.
3, after putting bolt the 13rd day, the 14th day, the 15th day, intramuscular injection hypophysis follicle stimulating hormone 60,50,40 units respectively.
4, after 15 days,, withdraw from the progesterone intravaginal sponge, the Cloprostenol of intramuscular injection simultaneously 0.2mg by tractive bolt line.
5, remove and adopt behind the bolt ram examination feelings method to determine that ewe oestruses in 1-4 days, No. 2 50 units of preceding 2 hours injection decorporations of breeding.
6, breeding was dashed and is got the embryo in back about 72 hours.
At present in the embryo engineering of boer goat, after the estrus induction of main employing Cloprostenol method, determine the feelings phase of ewe, injection follicle stimulating hormone before oestrusing, often administration time is difficult for grasping, and success rate is 50%, and individual variation is bigger, processing time is long, generally needs two feelings phases, promptly about 40 days.Use the inventive method, select 8 boer goats for use at Lishui, Nanjing sheep stud, 4 boer goats are handled with the inventive method, and the Progesterone consumption is 200mg/, makes oiliness progesterone intravaginal sponge.Removing bolt employing in preceding 3 days diminishing method, injection follicle stimulating hormone (promoting the medicine of follicle maturity) 150 units, after removing bolt, when identifying that ewe oestruses, breed and injected decorporation element (medicine that short ovum is discharged from the ovary surface) in preceding 2 hours, breeding cut open the belly in back 3 days gets the embryo and uses the microscopy embryo quality; Other 4 boer goats compare, after the estrus induction of Cloprostenol method, at the preceding 3 days injection follicle stimulating hormone of oestrusing next time, injection follicle stimulating hormone (promoting the medicine of follicle maturity) 150 units, after removing bolt, when identifying that ewe oestruses, the preceding 2 hours injection decorporation elements (medicine that short ovum is discharged from the ovary surface) of breeding, breeding cut open the belly in back 3 days gets the embryo and uses the microscopy embryo quality.In this experimental study, we obtain 43 pieces of available embryos from 4 boer goats, 100% sheep produces super row, be significantly higher than matched group and can use 29 pieces of embryos and effective percentage 50%, this result is beyond thought, and from the operating time, the inventive method only needs about 20 days, and prior art needs about 43 days.
Can count situation with ovulation point fetal development color born of the same parents born of the same parents born of the same parents cell blastaea embryo towards getting ovary 1 thin 4 thin 8 thin 16 unusual sheep
1 9 13 15 6 3 3 3 0 0 0 0 3 0 0 02 14 15 17 8 17 2 9 5 8 0 0 0 0 1 03 16 4 8 6 10 0 0 0 0 6 10 0 0 0 04 4 2 4 4 0 1 0 3 0 0 0 0 0 0 05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 06 17 12 11 9 17 3 3 5 9 0 0 0 0 1 57 0 0 0 0 0 0 0 0 0 0 0 0 0 0 08 12 8 10 7 11 5 3 2 8 0 0 0 0 0 0：1-4，5-8。
Claims (4)
1, a kind of intravaginal sponge of inducing ewe to oestrus of being used to, it is characterized in that: with the medicine Progesterone, consumption is that 200-500mg adds the cylindrical, conical of diameter 2-5 centimetre, high 3-4 centimetre to, in the irregularly shaped sponge of the square or equal volume of long 2-5 centimetre, wide 3-4 centimetre, make intravaginal sponge.
2, according to claim 1 a kind of as the intravaginal sponge of inducing ewe to oestrus, it is characterized in that: Progesterone 200-500mg is dissolved in the edible salad oil of 2mL, is added in the sponge or takes by weighing 200-500mg Progesterone dry powder, directly be added in the sponge.
3, according to claim 1 and 2 a kind of as the intravaginal sponge of inducing ewe to oestrus, it is characterized in that: spongy embolus band single line rope.
4, according to claim 3 a kind of as the intravaginal sponge of inducing ewe to oestrus, it is characterized in that: spongy embolus band single line rope.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00112505 CN1281689A (en) | 2000-09-04 | 2000-09-04 | Vaginal spongy suppository for iducing ewe estrus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00112505 CN1281689A (en) | 2000-09-04 | 2000-09-04 | Vaginal spongy suppository for iducing ewe estrus |
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| CN1281689A true CN1281689A (en) | 2001-01-31 |
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| CN 00112505 Pending CN1281689A (en) | 2000-09-04 | 2000-09-04 | Vaginal spongy suppository for iducing ewe estrus |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101006945B (en) * | 2005-09-28 | 2010-05-12 | 上海市计划生育科学研究所 | A vagina administration apparatus for animals, and its preparation method and application |
| CN102302443A (en) * | 2011-09-21 | 2012-01-04 | 天津市聚星康华医药科技有限公司 | Misoprostol vaginal sustained-release suppository and preparation method thereof |
| CN109329212A (en) * | 2018-12-05 | 2019-02-15 | 贵州大学 | Nubia Synchronization Oestrus in Goats method |
| CN111743661A (en) * | 2020-07-06 | 2020-10-09 | 河北北方学院 | Method for inducing synchronous estrus and artificial insemination of Hu sheep in batch production |
| CN113908114A (en) * | 2021-10-29 | 2022-01-11 | 宁波第二激素厂 | Flugesterone acetate vaginal sponge |
| CN113975226A (en) * | 2021-10-29 | 2022-01-28 | 宁波第二激素厂 | Preparation method of fluoprogesterone acetate vaginal sponge |
-
2000
- 2000-09-04 CN CN 00112505 patent/CN1281689A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101006945B (en) * | 2005-09-28 | 2010-05-12 | 上海市计划生育科学研究所 | A vagina administration apparatus for animals, and its preparation method and application |
| CN102302443A (en) * | 2011-09-21 | 2012-01-04 | 天津市聚星康华医药科技有限公司 | Misoprostol vaginal sustained-release suppository and preparation method thereof |
| CN102302443B (en) * | 2011-09-21 | 2014-04-23 | 天津市聚星康华医药科技有限公司 | Misoprostol vaginal sustained-release suppository and preparation method thereof |
| CN109329212A (en) * | 2018-12-05 | 2019-02-15 | 贵州大学 | Nubia Synchronization Oestrus in Goats method |
| CN111743661A (en) * | 2020-07-06 | 2020-10-09 | 河北北方学院 | Method for inducing synchronous estrus and artificial insemination of Hu sheep in batch production |
| CN113908114A (en) * | 2021-10-29 | 2022-01-11 | 宁波第二激素厂 | Flugesterone acetate vaginal sponge |
| CN113975226A (en) * | 2021-10-29 | 2022-01-28 | 宁波第二激素厂 | Preparation method of fluoprogesterone acetate vaginal sponge |
| CN113975226B (en) * | 2021-10-29 | 2024-02-09 | 宁波第二激素厂 | Preparation method of fluogesterone acetate vaginal sponge |
| CN113908114B (en) * | 2021-10-29 | 2024-02-09 | 宁波第二激素厂 | Fluogesterone acetate vaginal sponge |
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