CN1280306A - Super microscopic image analysis method and device - Google Patents
Super microscopic image analysis method and device Download PDFInfo
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- CN1280306A CN1280306A CN00105681A CN00105681A CN1280306A CN 1280306 A CN1280306 A CN 1280306A CN 00105681 A CN00105681 A CN 00105681A CN 00105681 A CN00105681 A CN 00105681A CN 1280306 A CN1280306 A CN 1280306A
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- 238000003703 image analysis method Methods 0.000 title claims description 5
- 230000003287 optical effect Effects 0.000 claims abstract description 18
- 238000012545 processing Methods 0.000 claims abstract description 12
- 238000004458 analytical method Methods 0.000 claims abstract description 11
- 230000008878 coupling Effects 0.000 claims abstract description 8
- 238000010168 coupling process Methods 0.000 claims abstract description 8
- 238000005859 coupling reaction Methods 0.000 claims abstract description 8
- 238000012544 monitoring process Methods 0.000 claims abstract description 5
- 230000000007 visual effect Effects 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims description 8
- 238000000879 optical micrograph Methods 0.000 claims description 5
- 230000005622 photoelectricity Effects 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 claims description 4
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 3
- 239000013307 optical fiber Substances 0.000 claims description 3
- 208000005168 Intussusception Diseases 0.000 claims description 2
- 239000000835 fiber Substances 0.000 claims description 2
- 238000010191 image analysis Methods 0.000 claims 3
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 238000005286 illumination Methods 0.000 claims 1
- 230000005693 optoelectronics Effects 0.000 abstract 1
- 238000005516 engineering process Methods 0.000 description 5
- 230000008901 benefit Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 230000003321 amplification Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000005272 metallurgy Methods 0.000 description 1
- 238000007431 microscopic evaluation Methods 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
Images
Abstract
First image is microscopic amplified in optical microscopic unit, and then acquired by and further electronically amplified in an optoelectronic coupling and amplifying unit. The amplified image is fed to color monitor for visual monitoring and fed to computerized processing unit for image recognition, analysis and output simultaneously. The intervention of computerized analysis unit makes it possible to obtain objective, stable and reliable conclusion.
Description
The present invention relates to a kind of super microscopic image analysis method and device thereof, particularly a kind of image analysis method and device thereof that combines and carry out image processing with the electron image treating apparatus by optical microscope.
Be widely used in the microscopic analysis of medical science, biology, metallurgy and many scientific researches, teaching field, general optical microscope or the electron microscope of adopting carries out, and mostly be visual Direct observation greatly, make the result according to user's experience and judge, so it is bigger influenced by subjectivity.Along with the electronic processing development of technology, micro-image is carried out electronic processing, especially by Computer Analysis, will increase work efficiency greatly and result's reliability.Therefore, for this purpose many and the design device begun to be applied to above-mentioned field.For example open CN1177113A of Chinese patent and international open WO9315428 have all related to and utilize the electronics harvester as the follow-up amplification of optical microscope, obtain the electronics display image of bigger multiplying power, thereby obtain more comfortable visual environment, be easy to differentiate more micro-details.Though above-mentioned technology indicates optics and can carry out useful combining with electronic technology, they are still with visualization, and carrying out subjective analysis is purpose, and it is objective, stable still can not to obtain, the analysis conclusion that reliability is higher.In recent years along with Computer Image Processing, the especially raising of image recognition technology and perfect is for comparatively perfect basis has been established in proposition of the present invention.
In view of the defective of above prior art, one object of the present invention is, the new combination of a kind of optics and electronic image capture technology is provided, and based on prior art, it has simple to operation, the advantage that image quality is higher;
Another object of the present invention is, a kind of method for subsequent processing and device thereof of electronic image is provided, and it can obtain objectively the analysis conclusion that reliability is higher by the intervention of Computer Analysis device.
Device of the present invention mainly comprises:
Micro-amplifying unit comprises lighting source and optical microphotograph optical projection system;
Photoelectricity coupling amplifying unit comprises photoelectrical coupler and pick-up lens, also can directly project to ccd image and gather face;
Electric controller;
Information process unit comprises multimedia computer and necessary external device thereof;
Display unit shows that as the necessary image result it has enough resolution and color display capabilities;
Print unit is used to print display message and analysis result;
Functional software is used for graphical analysis and processing;
Other complementary equipment, for example nonessential peripheral hardware such as uninterrupted power source, audio amplifier.
Below in conjunction with the accompanying drawing illustrated embodiment, the method for the invention and device are elaborated.
Fig. 1 is the principle of work synoptic diagram of the basic comprising unit of apparatus of the present invention;
Fig. 2 is the connection diagram of apparatus of the present invention electric control system;
Fig. 3 is the structural representation of photoelectricity coupling multiplying arrangement in apparatus of the present invention.
According to Fig. 1, the present invention at first carries out micro-amplification by optical microphotograph unit 11, and micro-light source adopts cold light source 12, can import by optical fiber.Adopting cold light source is in order to make observed sample be subjected to as far as possible little disturbance, to observe thereby can carry out biological tissue; Micro-imaging is gathered by photoelectricity coupling multiplying arrangement 13, this device comprises that optical conjugate zoom pipe (the applicant is with the separate case application) and camera constitute, this device is made further electronics with the image of optical microscope output and is amplified, make total enlargement ratio substantially exceed the optics enlargement ratio, forming submicroscopic of the present invention amplifies, although it can not provide further optical resolution, really provide great convenience for follow-up image monitoring and Computer Image Processing; The image one tunnel that this unit obtains is delivered to colour picture monitor 14 and is carried out visual monitoring, and another road is delivered to computer processing unit 15 and carried out the image recognition analysis, for example can carry out necessary comparison and handle, the outstanding or rectification in the part of image etc.; Simultaneously, result being sent into color display unit 16 shows and sends into colour print unit 17 and print.
According to Fig. 2, showed the connection signal of the electric control system 2 that is used for said apparatus, AC power enters electric control system 2 by safety apparatus 20, and one the tunnel exports 21 as working power is used for color monitor 211, computing machine 212, color printer 213, optical fiber power supply 214 and light fixture 215; Another road is exported 22 supply light electric couplers 221 as the 12V D.C. regulated power supply and is used for electron image and picks up; One the tunnel then supply with direct current zoom motor 231 as 8V D.C. regulated power supply output 23 again, simultaneously, this power supply 23 also is used for the power supply of digital display tube 241 and operating voltage display tube 242 as the power supply of the digital output unit 24 of enlargement ratio reference value; One group of power source special signal is used to control light transmitting fiber light intensity controller 25, and it is artificially controlled by light intensity control knob 251.
According to Fig. 3, as important structure feature of the present invention, be used to link up the optical coupling device of optics and electronic image processing, be mainly an optical conjugate zoom pipe, it intersects mutually by helical pitch groove and guide-track groove and cooperates driving lens combination zoom.Described optical conjugate zoom pipe has the lead set 31 and the guide rail sleeve 32 of mutual intussusception, and they support mutually by bearing 33,312 and can drive mutual coaxial rotation by drive motor by gear 313; Helical pitch groove 37 on the lead set 31,311 with guide rail sleeve 32 on guide rail 36,310 intersect cooperation respectively, and respectively by pilot pin 35,39 drive lens tubes 34,38 along guide rail sleeve 32 slides within, article two, helical pitch groove 37,311 track relation meets the optical conjugate principle, it makes lens drum 34,38 mutual relationship keeps optical conjugate, that is can continuously change make under the prerequisite of multiplying power by interface 314 towards from the object space image planes of Optics in Microscope object lens with by interface 315 towards project electronic image acquisition means 316, picture side's image planes as camera or CCD direction can not produce excessive drift, give and to find a view and follow-up electronic image capture brings great convenience, owing to the application of helical pitch groove structure, spiral as compared with the past Zoom structure has more simple to operation quick simultaneously, the advantage that zooming range is bigger.Obviously, the imaging on the above-mentioned picture side image planes is more much bigger than the optics image on the object space image planes, very helps monitoring observation and Computer Image Processing.
The foregoing description is as just a kind of signal of illustrating ultimate principle of the present invention.Obviously, according to above-mentioned principle, under the prerequisite of essence of the present invention, same domain technician can have many concrete structural designs.
Claims (5)
1. super microscopic image analysis method, it is characterized in that, at first carry out micro-amplification and obtain the image that optics amplifies by the optical microphotograph unit, described optics image is at first made further electronics by the collection of photoelectricity coupling multiplying arrangement and is amplified, described electronic image one tunnel is delivered to colour picture monitor and is carried out visual monitoring, and another road is delivered to computer processing unit and carried out the image recognition analysis.
2. according to the described method of claim 1, it is characterized in that described optical microphotograph unit is to adopt cold light source to carry out analyzed object illumination.
3. super microscopic image analysis device, it mainly comprises:
Micro-amplifying unit comprises lighting source and optical microphotograph optical projection system;
Photoelectricity coupling amplifying unit comprises photoelectrical coupler and pick-up lens or ccd image collection face;
Information process unit comprises multimedia computer and external device thereof;
And demonstration output unit;
It is characterized in that, lighting source with above-mentioned micro--photoelectricity coupling amplifying unit is connected, essential lighting source is provided, described photoelectrical coupler is connected with the microprojection system, it is by the object space image of optical conjugate zoom pipe reception from the microprojection system, and export picture side's image to pick-up lens or ccd image harvester formation electronic image, described electronic image signal one tunnel carries out status surveillance as pilot signal input monitor, another road is transferred to information process unit, carry out Flame Image Process by computing machine and function corresponding software, the picture signal after the demonstration output unit that is connected with information process unit will be handled shows or printout.
4. according to the described super microscopic image analysis device of claim 3, it is characterized in that described optical conjugate zoom pipe has the lead set and the guide rail sleeve of mutual intussusception, they support mutually by bearing and can drive mutual coaxial rotation by drive motor by gear; Helical pitch groove on the lead set cooperates with the guide rail on the guide rail sleeve is crossing respectively, and drive two groups of lens drums along the guide rail sleeve slides within by pilot pin respectively, article two, the track of helical pitch groove relation meets the optical conjugate principle, and it makes the mutual relationship of lens drum keep optical conjugate.
5. power control system that is used for the super microscopic image analysis device, it is characterized in that, AC power enters electric control system by safety apparatus, and one the tunnel is used for color monitor, computing machine, color printer, optical fiber power supply and light fixture as working power output; Another road is used for electron image as 12V D.C. regulated power supply output supply light electric coupler and picks up; One the tunnel then as 8V D.C. regulated power supply output supply direct current zoom motor again, and simultaneously, this power supply also is used for the power supply of digital display tube and operating voltage display tube as the power supply of the digital output unit of enlargement ratio reference value; One group of power source special signal is used to control light transmitting fiber light intensity controller, and it is artificially controlled by the light intensity control knob.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CNB001056816A CN1156721C (en) | 2000-04-17 | 2000-04-17 | Super microscopic image analysis method and device |
Applications Claiming Priority (1)
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CNB001056816A CN1156721C (en) | 2000-04-17 | 2000-04-17 | Super microscopic image analysis method and device |
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CN1280306A true CN1280306A (en) | 2001-01-17 |
CN1156721C CN1156721C (en) | 2004-07-07 |
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CNB001056816A Expired - Fee Related CN1156721C (en) | 2000-04-17 | 2000-04-17 | Super microscopic image analysis method and device |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102969878A (en) * | 2011-08-31 | 2013-03-13 | 佳能株式会社 | Line filter, switching power supply mounting line filter, and image forming apparatus |
CN103473462A (en) * | 2013-09-17 | 2013-12-25 | 北京克迷特技贸公司 | Ultra-microscopic image analysis method-based health transitioning and early-warning system |
-
2000
- 2000-04-17 CN CNB001056816A patent/CN1156721C/en not_active Expired - Fee Related
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102969878A (en) * | 2011-08-31 | 2013-03-13 | 佳能株式会社 | Line filter, switching power supply mounting line filter, and image forming apparatus |
CN102969878B (en) * | 2011-08-31 | 2016-04-13 | 佳能株式会社 | Line filter, the Switching Power Supply of carrying line filter and image processing system |
CN103473462A (en) * | 2013-09-17 | 2013-12-25 | 北京克迷特技贸公司 | Ultra-microscopic image analysis method-based health transitioning and early-warning system |
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Publication number | Publication date |
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CN1156721C (en) | 2004-07-07 |
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