CN1277264A - Reagent kit for enzyme process of determining beta-hydroxy butyric acid - Google Patents
Reagent kit for enzyme process of determining beta-hydroxy butyric acid Download PDFInfo
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- CN1277264A CN1277264A CN 99112967 CN99112967A CN1277264A CN 1277264 A CN1277264 A CN 1277264A CN 99112967 CN99112967 CN 99112967 CN 99112967 A CN99112967 A CN 99112967A CN 1277264 A CN1277264 A CN 1277264A
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- China
- Prior art keywords
- reagent kit
- butyric acid
- enzyme process
- hydroxy butyric
- determining beta
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Abstract
The reagent kit features that it connects trihydroxy methyl amino methane-hydrochloric acid buffering liquid, disodium complexonate, betahydroxybutyric acid dehydrogenase, oxidative nicotinamide adenine dinucleotide, magnesia, sodium azide and cane sugar. Compared with available similar reagent, the present invention has the features of long preservation period, high detection speed and easy use.
Description
The present invention relates to a kind of reagent kit for enzyme process of determining beta-hydroxy butyric acid.
Measure the blood urine β-Qiang Dingsuan to the diabetic ketoacidosis early diagnosis, instruct curative effect, judge that prevention is significant, also significant to the ketoacidosis that alcohol, hunger, eclampsia cause.Measuring β-Qiang Dingsuan has volumetry, hydrometer method, colorimetry, and these measuring methods are semiquantitative, do not have specificity with vapor-phase chromatography, and the capillary isotachophoresis method is unsuitable for clinical chemical examination in enormous quantities.Relevant document has been introduced the method for enzyme process of determining beta-hydroxy butyric acid, but this reagent can only be now with the current, can not prolonged preservation.
The object of the present invention is to provide a kind of finding speed fast, and the reagent kit for enzyme process of determining beta-hydroxy butyric acid of energy prolonged preservation.
Technical scheme of the present invention: a kind of reagent kit for enzyme process of determining beta-hydroxy butyric acid is characterized in that: contain Tutofusin tris-hydrochloride retarding agent, disodium ethylene diamine tetraacetate, β-Qiang Dingsuantuoqingmei, NAD, magnesium chloride, sodium azide, sucrose.
Beneficial effect of the present invention: disodium ethylene diamine tetraacetate is effectively closed heavy metal ion and is avoided destruction to enzyme in the Lip river in the present invention prescription, and magnesium chloride can be as the activator of enzyme, and sodium azide can be anticorrosion, and sucrose has provide protection to enzyme.Test kit has used enzyme to make catalyzer, so immunity from interference of the present invention is strong, moderate haemolysis, chylemia, blood sugar 20 mmoles/liter below, bilirubin 100 little mmoles/liter below do not produce interference to measuring.Freeze-dried reagent refrigeration 1 year, enzyme activity descends less than 5% in the test kit, and Reduced nicotinamide-adenine dinucleotide destroys less than 10%.Survey the β-Qiang Dingsuan linearity range, reach 5.0 mmoles/liter, rate of recovery 97.1-104.2%, the X=0.24 mmole/liter, n=20, CV=6.3%, SD=0.015 stablizes 72 hours (room temperature) after the redissolution, refrigeration was stablized for two weeks.
Embodiments of the invention are described in detail in detail below.
Get ultrapure distilled water, boil, seal standby with before heating water to.With Tutofusin tris-hydrochloride retarding agent, disodium ethylene diamine tetraacetate, magnesium chloride, sodium azide, sucrose adds aforesaid dissolved in distilled water, transfer pH value in 8.0, add β-Qiang Dingsuantuoqingmei then, NAD, at this moment, disodium ethylene diamine tetraacetate content 21 mmoles/liter, magnesium chloride 2 mmoles/liter, sodium azide 0.01 mmole/liter, β-Qiang Dingsuan deoxygenase 30 international unit/liter, NAD 2.0 mmoles/liter, Tutofusin tris-hydrochloride retarding agent 65 mmoles/liter, sucrose concentration 40 mmoles/liter, this reagent solution is packed as 5-10 milliliter/bottle, behind frozen drying, refrigerate, moisture content can be preserved more than 1 year less than 3% after the lyophilize.During use, add the dissolving of 5-10 ml distilled water and can use in 10 minutes.
Claims (2)
1, a kind of reagent kit for enzyme process of determining beta-hydroxy butyric acid is characterized in that: contain Tutofusin tris-hydrochloride retarding agent, disodium ethylene diamine tetraacetate, β-Qiang Dingsuantuoqingmei, NAD, magnesium chloride, sodium azide, sucrose.
2, reagent kit for enzyme process of determining beta-hydroxy butyric acid according to claim 1 is characterized in that: the mol ratio of each components contents is: Tutofusin tris-hydrochloride retarding agent 60-100, disodium ethylene diamine tetraacetate 20-30, NAD 1.8-4.0, magnesium chloride 1.0-5.0, sodium azide 0.009-0.015, sucrose 14.6-43.8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB991129679A CN1165627C (en) | 1999-05-28 | 1999-05-28 | Reagent kit for enzyme process of determining beta-hydroxy butyric acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB991129679A CN1165627C (en) | 1999-05-28 | 1999-05-28 | Reagent kit for enzyme process of determining beta-hydroxy butyric acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1277264A true CN1277264A (en) | 2000-12-20 |
| CN1165627C CN1165627C (en) | 2004-09-08 |
Family
ID=5276208
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB991129679A Expired - Fee Related CN1165627C (en) | 1999-05-28 | 1999-05-28 | Reagent kit for enzyme process of determining beta-hydroxy butyric acid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1165627C (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102435749A (en) * | 2011-09-02 | 2012-05-02 | 宁波美康生物科技股份有限公司 | Liquid stable kit for measuring beta-hydroxybutyric acid by cyclic enzyme method |
| CN104730230A (en) * | 2013-12-23 | 2015-06-24 | 上海复星医药（集团）股份有限公司 | Enzymatic method detection kit of D-3-hydroxybutyric acid and preparation method thereof |
| CN105510261A (en) * | 2015-11-30 | 2016-04-20 | 山东博科生物产业有限公司 | High-sensitivity D3-hydroxybutyric acid (D3H) detection reagent and detection method thereof |
| CN105842437A (en) * | 2016-04-28 | 2016-08-10 | 安徽伊普诺康生物技术股份有限公司 | Kit for detecting D-3-hydroxybutyric acid and preparation method of kit |
| CN107991252A (en) * | 2017-11-23 | 2018-05-04 | 中山市创艺生化工程有限公司 | A kind of stabilizer for α-hydroxybutyrate dehydrogenase assay kit and preparation method thereof |
-
1999
- 1999-05-28 CN CNB991129679A patent/CN1165627C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102435749A (en) * | 2011-09-02 | 2012-05-02 | 宁波美康生物科技股份有限公司 | Liquid stable kit for measuring beta-hydroxybutyric acid by cyclic enzyme method |
| CN102435749B (en) * | 2011-09-02 | 2013-10-16 | 宁波美康生物科技股份有限公司 | Liquid stable kit for measuring beta-hydroxybutyric acid by cyclic enzyme method |
| CN104730230A (en) * | 2013-12-23 | 2015-06-24 | 上海复星医药（集团）股份有限公司 | Enzymatic method detection kit of D-3-hydroxybutyric acid and preparation method thereof |
| CN105510261A (en) * | 2015-11-30 | 2016-04-20 | 山东博科生物产业有限公司 | High-sensitivity D3-hydroxybutyric acid (D3H) detection reagent and detection method thereof |
| CN105842437A (en) * | 2016-04-28 | 2016-08-10 | 安徽伊普诺康生物技术股份有限公司 | Kit for detecting D-3-hydroxybutyric acid and preparation method of kit |
| CN107991252A (en) * | 2017-11-23 | 2018-05-04 | 中山市创艺生化工程有限公司 | A kind of stabilizer for α-hydroxybutyrate dehydrogenase assay kit and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1165627C (en) | 2004-09-08 |
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