CN1270816A - Youth and longevity pellet and its preparation - Google Patents
Youth and longevity pellet and its preparation Download PDFInfo
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Abstract
Youth and longevity pellet has the recipe comprising epimedium, gynostemma pentaphylla, Lycium chinense and tea, of which the effective components are extracted by means of specific technological process. It has comprehensive and high disease curing effect and little toxic side-effect. Its medicine source is extensive and its cost low.
Description
The present invention relates to the vigor of renewing one's youth, the medicine of life-saving, especially a kind of Youth and longevity pellet.
With the vigor of renewing one's youth, life-saving is that the medicine and the health product of purpose are a lot of at present, but effect is not strong mostly, and costs an arm and a leg, and the ordinary people can not consume.Middle-aged and elderly people, various diseases is burdened with, need a kind of Comprehensive Treatment medicine, geromorphism, worn with age, neurasthenia, fatigue both can have been treated, in, old man, women's hypofunction or obstacle, he cardiovascular and cerebrovascular disease, myocardial infarction, cerebral infarction, heart failure is in can preventing and treating again, old osteitis, bone regeneration, osteonecrosis, in the control, old hepatitis, gastric ulcer, difficult miscellaneous diseases such as control bronchitis, asthma, emphysema, pulmonary heart disease, senile dementia.And at present, also do not have this medicine and can satisfy the Comprehensive Treatment purpose.
The purpose of this invention is to provide a kind of combined therapy effect that has, curative effect height, the Youth and longevity pellet that toxic and side effects is little, the medicine source is wide, cheap.
For achieving the above object, the present invention is by the following technical solutions:
The prescription of Youth and longevity pellet consists of Herba Epimedii, Herb Gynostemmae Pentaphylli, Fructus Lycii, Folium Camelliae sinensis.
The preparation method of this Youth and longevity pellet is realized by following concrete reparation technology:
(1) Herba Epimedii selenium extraction process:
(1) crude product Herba Epimedii selenium extraction process: the former medicine of crude product adds 8 times of amount 1% selenic acid liquid, kept 80 ℃ of reflux, extract, 3 hours, the leaching medicinal liquid is put in addition, residue adds 5 times of amount 1% selenic acid liquid again, pressing the Same Way operation extracted 2 hours, multiple leaching medicinal liquid and preceding liquid merge, and gather all selenic acid extracting solution, are condensed into than weighing 1.1～1.2, add 95% ethanol equivalent precipitated impurities, left standstill 12～14 hours, and extracted supernatant liquid filtering, precipitation is with 45% washing with alcohol 2～3 times, the solubility composition washes out in will precipitating, filtrate and washing liquid merge, and reclaim ethanol, and concentrating under reduced pressure is cooled to cream; Promptly get the thick extracted extract of Herba Epimedii selenium;
(2) pure product Herba Epimedii selenium extraction process: the thick extracted extract of Herba Epimedii selenium adds 70%4 times of amount ethanol, and reflux 2 hours is filtered, and filtering residue adds 2 times of amount alcohol refluxs 1 hour again, filters, and reclaims ethanol, and 60 ℃ of dryings promptly get pure product Herba Epimedii selenium;
(3) epimedium brevicornum polysaccharide selenium extraction process:
Herba Epimedii is pulverized coarse powder and is added 5 times of amounts of 75% ethanol, 0.5% selenic acid (Ha2SeO4) reflux 2 hours, filter, extract 3 times with method, merging filtrate is condensed into cream, adds 95% ethanol with being stirred to precipitation, 12 hours after-filtration, reclaim ethanol, continuous 2 times, add 2 times of amounts of water, with DEAE cellulose (phosphate type) column chromatography purification, get pure product epimedium brevicornum polysaccharide selenium;
(2) Herb Gynostemmae Pentaphylli total glycosides chromium extraction process:
Natural Herb Gynostemmae Pentaphylli is a raw material, filter with 38 times of water gaging lixiviates of 1% chromic oxide gel Cr (OH), extract 3 times continuously, merging filtrate adopts the resin adsorption method production technology, selects for use D101 type adsorbent resin and nontoxic ethanol to take off agent as washing liquid, other has the macro-reticular adsorbing resin method to extract gypenoside, adopt 1%Cr (OH) 3 floodings, D type non-polar resin adsorption and purification, activated carbon decolorizing;
1. extract with 50% ethanol, the medicinal liquid cocnentration factor weighs 1.14 before the precipitate with ethanol, and determining alcohol is 70%, adds 3 times of water gagings of medicinal liquid during water precipitating, the oral liquid clarity of preparation is taken a turn for the better, and gypenoside content is higher;
2. medicinal liquid degree of being condensed into before the precipitate with ethanol, the medicinal liquid ratio weighs 1.25, and PH is 4.20, and mixing time is 1.5 hours;
(3) Fructus Lycii extracts Folium hydrangeae strigosae alkali, lycium barbarum polysaccharide zinc extraction process:
Getting former medicine by the seepage method, with moistening 4 hours of 1% zinc sulfate, 45% ethanol, is solvent dipping 24 hours with 1% zinc sulfate, 45% ethanol, with the speed seepage of per minute 50ml/5kg, when making transudate reach 7 times of amounts of crude drug, promptly stop seepage, collection is got sepage, reclaims ethanol, and thickening filtration; Be adjusted to every ml and contain total amount 150mg, add 0.25% benzoic acid again, 0.1% nipalgin is anticorrosion, leaves standstill 8～10 days, gets the supernatant and filters, and, is dried into dry extract for 60 ℃ that is;
(4) Folium Camelliae sinensis extracts theophylline and caffeine technology:
Folium Camelliae sinensis adds 8 times of amount 1% sulphuric acid liquid and soaks seepage after 14 hours, continuous 3 times, merging filtrate is concentrated into original volume 1/5 and filters, and filtrate transfers to PH7 with ammonium hydroxide (NH4OH), add 95% ethanol equivalent precipitated impurities, left standstill 12 hours, and extracted supernatant liquid filtering out, precipitation is with 45% washing with alcohol 2～3 times, be condensed into cream, promptly get theophylline and caffeine crystallization;
(5) preparation process of Youth and longevity pellet:
Herba Epimedii selenium 50mg/kg/ day, epimedium brevicornum polysaccharide selenium 20mg/kg/ day; Herb Gynostemmae Pentaphylli total glycosides chromium 2.5mg/kg/ day; Fructus Lycii consumption extract Fructus Lycii is extracted betanin 1.5mg/kg/ day, lycium barbarum polysaccharide zinc 1.5mg/kg/ day; Folium Camelliae sinensis extract theophylline 8mg/kg/ day, caffeine 4mg/kg/ day; Altogether 4375mg/50kg/ day, be divided into 10 large capsules, each capsule contains 437.5mg.
Below introduce the effect that the present invention has by pharmacology, the drug effect of each composition:
One, the medicine characteristics of Youth and longevity pellet:
1. we have novelty, reasonable recipe.Have efficient, low toxicity, the minimum characteristics of side effect.With the kidney invigorating, invigorate blood circulation, heart tonifying, raise immunity and resistance, the vigor of renewing one's youth, life-saving.Form by medicines such as Herba Epimedii, Herb Gynostemmae Pentaphylli, Fructus Lycii, Folium Camelliae sinensis.Through clinical treatment 1166 routine various patients, cardiovascular and cerebrovascular disease, neurasthenia, sexual hypofunction, tracheitis, alzheimer disease, diabetes, hepatitis, gastropathy, prevention and treatment cancer, tumor all play good effect.1. quick-acting: taking 12～24 hours is visible positive effect.2. after 99% people takes medicine very good effect is arranged.3. nontoxic: as to take 1～3 month according to 1166 people, toxic and side effects do not occur.4. there is not addiction.5. there is not hormone.
2. our extraction process advanced person: with trace element soda acid novel process for purifying, extraction ratio height, and curative effect improves 30～50%.For example 1. chromic oxide gel extracts Herb Gynostemmae Pentaphylli, and the effect of Herb Gynostemmae Pentaphylli blood vessel dilating is strengthened, and prevent atherosclerosis, and can strengthen glucose tolerance factor, and blood sugar lowering.2. extract icariine selenium and epimedium brevicornum polysaccharide selenium with selenic acid liquid, can make Herba Epimedii selenium cardiovascular and cerebrovascularinfarction district cell be repaired as early as possible and regenerate, and anti-cancer be arranged and control the cancer effect.3. extract Fructus Lycii betanin and lycium barbarum polysaccharide zinc with zinc sulfate, both played the kidney invigorating, nootropics and libido function, again can raise immunity, treatment of arthritis, alzheimer disease, lumbago and skelalgia, treatment liver, gastropathy, extraction process changes curative effect and improves 30～50% thus.
3. anti-aging effects: prolong cell survival, anti-hypoxia, resisting fatigue are applicable to worn with age, neurasthenia.
4. this medicine can promote sexual function: be because gonepoiesis is hyperfunction, and after seminal vesicle is full of, the sensation nerve, indirect excitation libido and causing can increase prostate, seminal vesicle, levator ani weight, promotes testis and epididymis to grow.The hypersexuality function strengthens women uterus, ovary weight, postpones climacteric.In being applicable to, senile hypofunction and obstacle etc.
5. coronary artery dilator and cardiotonic are arranged: be applicable to coronary heart disease and heart failure.
6. blood fat reducing and blood pressure effect are arranged: be applicable to treatment and prevention of arterial atherosis with hypertension, cardiovascular and cerebrovascular disease.
7. anticoagulant is arranged: can prevent and treat heart and brain thrombosis, myocardial infarction, cerebral infarction, cardiovascular and cerebrovascular vessel patient's prevention and treatment.
8. immunostimulant, immunosuppressant, dual regulation are arranged: can induce r-globulin and interleukin II and produce, so to immunologic hypofunction, the valetudinarian has better curative effect.
9. hypoxia-bearing capability is arranged: prolong the cell survival time-to-live, promote protein and nucleic acid metabolism, promote the normal bone growth, in the control, the generation of old osteitis, bone regeneration, osteonecrosis.
10. have strengthen glucose tolerance factor, strengthen insulin active, excreting insulin and blood sugar lowering.
11. have protect the liver, antiulcer action: in the control, the generation of old hepatitis and gastric ulcer.
12. the expansion bronchus smooth muscle is arranged, cardiotonic: be applicable to treatment bronchitis, asthma, pulmonary heart disease, emophysematous generation.
13. calmness, hypnosis, analgesia, excited cerebral cortex, hypermnesis function: control senile insomnia, lumbago and skelalgia, brain atrophy, alzheimer disease.
14. prevention and treatment tumor: because of containing dimension secondary element selenium, so the effect of prevention and treatment tumor is arranged.
15. resisting pathogenic microbes effect, antiinflammatory action, antihistaminic effect: so can prevent and treat pathogenic microorganism infringement, the infection, swelling, the granulation tissue hyperplasia that diminish inflammation and cause.
16. long this medicine of clothes, health invigorating is prevented and treated the generation of various diseases, recovers one's youthful vigour, and youth length exists life lengthening.
Two, the pharmacological action of Youth and longevity pellet:
The pharmacological action of Herba Epimedii
(1) anti-aging effects: subcutaneous injection D-galactose can cause subacute aging model in 40mg/kg68 days next day of male mice.In injection of d-galactose, irritate stomach epimedium flavone 0.7g/kg and can significantly recover the function of D-galactose aging model mice T and bone-marrow-derived lymphocyte breeder reaction, obviously improve the activity of the total SOD of mouse liver, reduce the formation of hepatic tissue lipid peroxide, the lipofuscin that reduces tissues such as the heart, liver forms, but reduces not obvious to the lipofuscin of brain.The compound preparation of Herba Epimedii " ERXIANJIAO capsule " suspension, can prolong the silkworm larva phase, pupa time, adult stage and total age in longevity, 1.25g/kg.d administration, can reduce the lipofuscin content in mouse brain, the heart, the liver, IgG, total complement level increase in the blood, and neutrophilic granulocyte and peritoneal macrophage phagocytic function strengthen.
(2) androgen sample effect: give injected in mice Herba Epimedii preparation, can make the important increase of prostate, seminal vesicle and levator ani, prove the effect of androgen sample, render a service than a little less than the Fructus Cnidii, strong than Gecko and Hippocampus.Can promote the excretory effect of dog sperm; The strongest with leaf and the effect of root preparation, behind the application Herba Epimedii, the excretion of 17-KS also obviously increases in the urine, illustrates having certain effect aspect the promotion gonad function.
(3) coronary artery dilator: the Herba Epimedii decoct can increase the coronary flow of guinea pig isolated heart.The non-amino acid moiety that extracts in its aqueous extract sheet can increase the coronary flow of the extrasomatic rabbit heart and the anesthetized dog heart, reduces the coronary resistance of the dog heart, to not obviously influence of heart rate; The Acute Myocardial Ischemia in Rats that pituitrin is caused has protective effect, and can shorten epinephrine or the caused experimental arrhythmia of strophantin K significantly.
(4) blood pressure lowering: pure preserved material and methanolic extract, anesthetized rabbit have obvious and persistent hypotensive activity, and methanolic extract is given normal and the hypertensive rats gavaged of kidney type, tangible blood pressure drops also occurs; Give the cat lumbar injection, can make the blood pressure of a part of cat that reduction is to some extent arranged.Because it can weaken pressor reflex significantly and the effect of expansion rabbit ear blood vessel is arranged, its hypotensive effect may be direct blood vessel dilating and the dual reason that suppresses vasomotor center.(5) antitussive, eliminate the phlegm, relieving asthma: prove that with the phenol red excretion method of mice the aquatic foods product crude extract of Herba Epimedii and the ethyl acetate extract of dry product have certain phlegm-dispelling functions.Drawing the method for coughing with mice sulfur dioxide proves, methanol and ethyl acetate extract have antitussive effect; Methanolic extract can also suppress to stimulate the cough due to the cat superior laryngeal nerve.Antitussive is central.Methanolic extract has protective effect to drug-induced asthma.
(6) antibiotic and antiviral: white and staphylococcus aureus, streptococcus pneumoniae, Nai Shi micrococcus catarrhalis, hemophilus influenza, tubercule bacillus are had inhibitory action.Poliovirus and some enterovirus have inhibitory action.(7) antiinflammatory: methanolic extract can alleviate the swelling degree of rat Ovum Gallus domesticus album " arthritis " significantly.Reduce the phenomenon that the thin vascular permeability of the rabbit hair due to the histamine increases, can increase the phagocytic activity of mice inflammatory exudation cell.Increase mice and under normal pressure, tolerate anoxybiotic ability.The extracting solution of Herba Epimedii can also make the blood glucose of experimental hyperglycemia rat obviously reduce.
(8) pharmacokinetics: trace manganese is in the dynamic (dynamical) research of generation of Sanguis Leporis seu oryctolagi Chinese medicine in the Herba Epimedii, with the Catalytic-Kinetic Analysis is monitoring means, utilize the catalytic action of divalent manganesetion to Potassium metaperiodate .-rhodamine B reaction system, use Phen to be activator, trace manganese in the Sanguis Leporis seu oryctolagi has been carried out fluoremetry, curve when having drawn medicine, the result is through MICROCOMPUTER PROCESSING, physiological disposition meets two Room open models, and has obtained serial pharmacokinetic parameter.
(9) toxic and side effects: the LD50 of Herba Epimedii extractum mouse peritoneal injection is 36g/kg.The LD50 of the quiet notes of non-amino acid moiety (No5-1-1) mice that extract in the aqueous extract sheet is 56.8 ± 2.7g/kg.The toxicity of methanolic extract is low, and mouse gavaging 450g/kg observed 3 days continuously, and activity as a result is normal, non-toxic reaction.
The acute toxicity test of mouse peritoneal injection Herba Epimedii total flavones, LD50 is 2.99 ± 0.14g/kg.Acute and sub-acute toxicity test shows that Herba Epimedii extracting solution various dose is taken continuously, animal liver, kidney, the heart, spleen etc. is organized all not have obviously change, and mode of appearance is normal.
Mouse peritoneal injection various dose total flavones is observed each dosage treated animal per cent death loss in 24 hours, and trying to achieve total flavones LD50 with the improvement karber's method is 2.99 ± 0.14g/kg.
The mutagenicity study of epimedium brevicornum polysaccharide, the inside and outside every mutagenicity test of effective aspect shows, does not see that EPS has mutagenicity.
The pharmacological action of Herb Gynostemmae Pentaphylli
(1) anti-aging effects:
1. prolongation cell survival: Herb Gynostemmae Pentaphylli can prolong the cell proliferation of forming organism and go down to posterity.In the culture fluid that contains Herb Gynostemmae Pentaphylli total glycosides 200 μ g/ml, human fetal exsomatizes normal diploid fibroblast (WI-1) procreation to 59 generations, and only reaching for 51 generations, matched group just stopped generation, prolong 15.7%, to Werner syndrome patient's Skin Cell isolated culture, matched group only multiplied for 22 generations, and the Herb Gynostemmae Pentaphylli group extended to for 27 generations, prolong 22.7%, therefore the title of cell vitalizing agent is arranged.
2. prolong the animal life-span: Herb Gynostemmae Pentaphylli extract, general glycoside and compound preparation all can significant prolongation average life and the maximum life span of male and female fruit bat.0.25%, the effect and the vitamin E of the average life of 0.5% and 1% Herb Gynostemmae Pentaphylli water-alcohol extraction prolongation fruit bat are suitable.0.5% extract can also make fruit bat turn into the time shortening of adult from ovum, can prolong the life-span in adult stage again, more obvious since 30 days adult administering effect, show that Herb Gynostemmae Pentaphylli had both promoted growth promoter, delayed aging course again, pointed out simultaneously that to begin defying age from the embryo be feasible.The study of the subacute aging model of mice that Herb Gynostemmae Pentaphylli and compound preparation thereof cause the d-galactose initiatively decline of escape reaction ability has significant antagonism.To contain the feedstuff feed Aged Mice of 4% Herb Gynostemmae Pentaphylli water extract, survival rate is 50%, and matched group is 0%, and there were significant differences.
3. anti-aging effects mechanism: Herb Gynostemmae Pentaphylli water extract 200mg/kg admixed feedstuff feed mice childhood three months, can make that lipofuscin content significantly descends in brain, the heart tissue, and the lipofuscin in the liver also has decline; 0.5%, two first quarter moons of 0.25% Herb Gynostemmae Pentaphylli water extract feed can make the senile rat heart, liver, cerebral tissue lipid peroxide significantly reduce.Rat blood serum and total cholesterol of liver, triglyceride all have obvious reduction effect.Herb Gynostemmae Pentaphylli total glycosides can reduce the content of lipid peroxide in mice plasma, liver, the brain (LPO), improves the activity of superoxide dismutase (SOD) in liver, the brain.Herb Gynostemmae Pentaphylli and compound recipe thereof can resist the collection that increases of the unusual rising of aging model group mouse brain MAO-B vigor and brain lipofuscin significantly, can improve mouse immune power, make the thymus of aging model mice atrophy return to normal level.Herb Gynostemmae Pentaphylli can also reduce LPO content in the human body significantly.The anti-aging effects of prompting Herb Gynostemmae Pentaphylli is realized by many-sided effect.
(2) to the effect of nonspecific stimulation:
1. antifatigue effect: Herb Gynostemmae Pentaphylli water extract, Saponin, general glycoside all can significant prolongation mice swimming time and pole-climbing times, show antifatigue effect.The Herb Gynostemmae Pentaphylli extract of 200mg/kg is irritated stomach, and continuous 14 days, can make long-time swimming rat keep higher blood sugar level, reduce the loss of inose unit, this may be a Herb Gynostemmae Pentaphylli antifatigue major reason.
2. oxygen lack resistant function: the be improved normal pressure resisting oxygen lack of mice of Herb Gynostemmae Pentaphylli extract, Saponin, general glycoside obviously prolongs the mice hypoxia endurance time.
3. high temperature resistance, cold service.
(3) to the influence of organism metabolism:
1. effect for reducing blood fat: 0.5%, 0.25% Herb Gynostemmae Pentaphylli water extract all has obvious decline effect to rat blood serum and total cholesterol of liver, triglyceride.T-CHOL in the blood (TC) is 159.0 ± 19.0mg/dl, and triglyceride (TG) mean concentration is 234.4 ± 18.1mg/dl, adds Herb Gynostemmae Pentaphylli total glycosides 100mg/kg.d * 7 weeks, and its TC and TG are respectively 107.9 ± 8.8 and 153.6 ± 9.1mg/dl.The remarkable effect for reducing fat of Herb Gynostemmae Pentaphylli is and suppresses adipose cell generation free fatty and synthesize neutral fat relevant.It is gypenoside G, I, J, K and former gypenoside that proof has the composition of blood fat reducing.
2. blood sugar lowering and improve carbohydrate metabolism: Herb Gynostemmae Pentaphylli extract 200mg/kg, 100mg/kg irritate stomach alloxan diabetes white mice, continuous three days, obvious hypoglycemic activity are arranged; And it is low obviously to improve senile rat blood glucose tolerance.Herb Gynostemmae Pentaphylli can be improved the synthesis rate of spleen, testis, brain and blood protein.
(4) to immune influence:
1. to the influence of nonspecific immunity: Herb Gynostemmae Pentaphylli can increase the weight of normal mouse spleen and thymus, and gypenoside 200,400mg/kg can also resist the caused by cyclophosphamide mouse spleen and thymic weight descends.Report is also arranged, and Herb Gynostemmae Pentaphylli total glycosides 300mg/kg irritates stomach does not all have obvious influence to normal mouse thymus, spleen weight.Mice is drunk Herb Gynostemmae Pentaphylli total glycosides extractum after January, and the volume of pulmonary alveolar macrophage obviously increases.Engulfing digestion power significantly strengthens.Herb Gynostemmae Pentaphylli extract can strengthen the intraperitoneal mouse macrophage phagocytic function, obviously improve colloid carbon granules clearance rate in the mice blood, Herb Gynostemmae Pentaphylli total glycosides can improve normal mouse peripheral leukocytes number and strengthen it values of chemiluminescence when engulfing zymosan, and can reverse the reduction that leukocyte count purpose that mice causes because of the injection prednisolone acetate reduces and engulfs luminous value.Engulfing luminous index (engulfing luminous value/leukocyte count) simultaneously shows: engulf the increase of luminous value and be not only owing to improved the leukocyte number and cause, and main be the phagocytic activity that has strengthened leukocyte self.
2. pair cell Immune Effects: Herb Gynostemmae Pentaphylli extract is fed 90 days rat, and its T lymphocyte number increases significantly.Herb Gynostemmae Pentaphylli water decoction 10g/kg, 30g/kg irritate stomach, can obviously resist exudative inflammation and strengthen the cell-mediated DTH reaction of T.Herb Gynostemmae Pentaphylli can be improved the positive percent of the acid a-naphthyl acetate esterase (ANAE) of white mice tail blood lymphocyte.Herb Gynostemmae Pentaphylli total glycosides 400mg/kg irritates stomach, and decline has obvious antagonism to cycli phosphate amine induced mice E-Flos Rosae Rugosae formation rate.Observe with myocardium transplant experiment method, significantly suppress the rejection of transplanting after the Herb Gynostemmae Pentaphylli total glycosides coloclysis.
3. to the influence of humoral immune function: Herb Gynostemmae Pentaphylli total glycosides can reduce hemolysin content, and the serum hemolysin that can recover caused by cyclophosphamide descends, Herb Gynostemmae Pentaphylli water decoction 10, and 30g/kg irritates stomach, can improve mice specific antibody hemolysin content.Herb Gynostemmae Pentaphylli total glycosides 300mg/kg irritates stomach, can significantly improve mice serum IgG content, and general glycoside II can significantly improve IgG and IgM content.The Herb Gynostemmae Pentaphylli total glycosides subcutaneous injection can strengthen normal mouse and caused by cyclophosphamide immunocompromised mice by the breeder reaction of the inductive spleen bone-marrow-derived lymphocyte of LPS.
4. to natural killer cell (NK) with bring out the effect of interferon: Herb Gynostemmae Pentaphylli total glycosides (100～200 μ g/ml) within the specific limits can promote the NK activity, and when 400 μ g/ml the NK activity is had inhibitory action.Herb Gynostemmae Pentaphylli total glycosides 400mg/kg irritates stomach, can obviously resist cyclophosphamide to the active inhibitory action of NK.The Herb Gynostemmae Pentaphylli total glycosides subcutaneous injection can improve the generation of splenocyte interleukin II (IL-2).
5. immunoregulation effect: gypenoside can make mouse immune device palace spleen weight, anti-SRBC hemolysin level and active E-Flos Rosae Rugosae formation rate be higher than the decline of median, and is lower than the rising of median, shows dual regulation.
(5) antitumor action: gypenoside all has the inhibition effect to stomach, rectum, mammary gland, uterus, oral cavity, esophagus, gallbladder, bile duct, pancreas, liver, brain, lung, kidney, tongue, thymus, thyroid, prostate, skin carcinoma and other tumor etc.Propagation to cancerous cell such as this hepatocarcinoma of Morley, uterus carcinoma, pulmonary carcinoma and melanosarcomas has remarkable inhibitory action, and suppression ratio is 20～80%.Saponin XXVII can also prolong mice average life when suppressing ehrlich ascites carcinoma, extends to 23 days from 15 days, and gypenoside is to the normal cell free of toxic effects.Experiment in vitro proof gypenoside has directly kills the effect of S180 sarcoma cell, and the killing rate of concentration 0.38～0.75% is 54～87.5%.
(6) to central nervous system's effect:
1. calm, syngignoscism: Herb Gynostemmae Pentaphylli can suppress the spontaneous activity of mice.Gynostemma pentaphyllum extract 450mg/kg and the compound gynostemma pentaphylla that contains equivalent are irritated stomach, all can suppress the spontaneous activity of mice after 0.75 hour, 3 hours peakings, and effect was kept more than 7 hours.
2. analgesic activity: Herb Gynostemmae Pentaphylli extract 100mg/kg can obviously suppress acetic acid induced mice writhing response, and hot plate method shows that 450mg/kg general glycoside extractum has significant analgesic activity to mice.
3. antipsycholic action: be placed on 130 times/minute the device that trembles concussion when mice 210 minutes, make animal in tension, the anus temperature of having a rest 1 hour is 35.1 ℃ and all slides on 45 landslides of spending, shows that animal is still in tension; The anus temperature of general glycoside 50 and 100mg/kg treatment group is respectively 36.0 and 36.2 ℃, and self-possessed being parked on the landslide safely of 51.2% and 52.3% animal, illustrates that Herb Gynostemmae Pentaphylli has tangible antitonic state.
4. to the facilitation of learning and memory: Herb Gynostemmae Pentaphylli water, 20%, 95% ethanol extract 3g/kg can improve Anisodine induced mice memory acquisition disturbance, Herb Gynostemmae Pentaphylli 20% ethanol extract can also resist the memory that protein synthesis inhibitor cycloheximide, the mould index of chlorine rise and consolidate obstacle, and memory obtains and reproduces bad due to 20% ethanol.
(7) to the effect of cardiovascular system:
1. to action of the heart: Herb Gynostemmae Pentaphylli water extract has the effect that increases coronary flow to extrasomatic rabbit heart, and extrasomatic rabbit heart is also had the obvious suppression effect, and decreased heart rate reduces the heart contraction amplitude.Its effect can be blocked by propranolol or weaken, and demonstration may be worked by adrenergic.A Herb Gynostemmae Pentaphylli total glycosides 5mg/ intravenous injection has better protective effect to the Bufo siccus myocardial ischemia that pituitrin causes.Herb Gynostemmae Pentaphylli total glycosides 40mg/kg, stable 5mg/kg, the treatment poisoning by lidocaine can obviously prolong the mice time-to-live, and reduce its mortality rate.
2. to the influence of blood vessel, blood pressure: Herb Gynostemmae Pentaphylli water extract 40% and 150% various dose show the lax aortal smooth muscle of low dosage to rabbit isolated aorta bar, and high dose shrinks aortal smooth muscle.20%, 40% intravenously administrable can be expanded the Canis familiaris L. peripheral blood vessel, the cerebral blood flow increasing amount, and Herb Gynostemmae Pentaphylli total glycosides 50mg/kg intravenous injection is remarkable hypotensive effect to anesthetized cat, hold time more than 30 minutes, and blood pressure drops degree and dosage is dependence.
3. to the influence of blood system: Herb Gynostemmae Pentaphylli total glycosides 50mg/kg subcutaneous injection, the rat experiment thrombosis there is obvious inhibitory action, wherein make the blood plate thrombus average magnitude descend 34%, phlebothrombosis descends 68%, Herb Gynostemmae Pentaphylli extract 0.25～2g/L, rabbit platelet aggregation and thromboxane B2 at external obvious inhibition arachidonic acid-induction discharge, dosage is relevant with effect, its ID50 that suppresses platelet release thromboxane B2 is 0.28g/L, behind the intravenous injection Herb Gynostemmae Pentaphylli extract 35mg/kg 10 and during 20min, platelet aggregation obviously is suppressed, platelet release thromboxane B2 amount obviously reduces during 10～40min, with 10 and the most remarkable during 20min, (0.25～4g/L) discharges 6-ketone-PGF1a influence to the rabbit thoracic aorta report is also arranged external this medicine, Herb Gynostemmae Pentaphylli total glycosides has obvious inhibitory action to the generation of rabbit rich platelet TXB2 and 6-ketone-PGF1a, its IC50 is respectively 1.03 and 1.15mg/ml, the electron microscopic observation Herb Gynostemmae Pentaphylli total glycosides does not have influence to tame rabbit platelet structure, but can suppress ADP, AA, the change of the inductive platelet profile of Collagen, the formation of pseudopodium and particulate release have further confirmed the inhibitory action of Herb Gynostemmae Pentaphylli total glycosides to platelet function.Contain PAF among the hot water extract of Herb Gynostemmae Pentaphylli, this aggregation can be stoped by prostacyclin I2 fully, but is not subjected to the influence of aspirin, meets β-Gluconase, this factor inactivation, but meet then not passivation of protease.The Herb Gynostemmae Pentaphylli alcohol extract is pressed 400mg/kg and is irritated stomach, and normal mouse plasma cAMP content is reduced, and cGMP content raises, and the ratio of cAMP/cGMP reduces.To human erythrocyte membrane Na+, the K+-ATP enzyme plays activation to gypenoside in the finite concentration scope.Granulocytopenia to caused by cyclophosphamide has the rising effect.
(8) to endocrine influence:
1. to the influence of gonad and sexual activity: Herb Gynostemmae Pentaphylli extract is irritated the weight that stomach can significantly increase white mice testis, seminal vesicle, prostate and uterus, and prompting has gonadotropic Effect.Its epididymis of senile rat and the testis of taking Herb Gynostemmae Pentaphylli obviously increase weight than the senile rat matched group.
2. the side effect of control glucocorticoid: gypenoside 10mg/kg lumbar injection, blood plasma cortisol content reduces after suppressing the rat adrenal gland, atrophy of thymus gland due to the dexamethasone, inhibitory action is irrelevant with the precedence that gives glucocorticoid, gypenoside not only can make the caused organ atrophy of glucocorticoid restore, but also can stop the appearance of this effect.Herb Gynostemmae Pentaphylli total glycosides 70,350mg/kg irritate stomach, can not only recover the mice atrophy of adrenal gland due to the dexamethasone, and can recover adrenal gland's Vitamin C content, histology and histochemistry are observed and are shown, Herb Gynostemmae Pentaphylli is to the adrenal gland's cryptomere band attenuation due to the dexamethasone, the cellular atrophy of outer cryptomere band, the pyknosis of capsule clothing, hole sample telangiectasis between the cell rope and kytoplasm lactone drip content minimizing etc. all the obvious suppression effect.
3. to the influence of plasma ACTH: the lumbar injection Herb Gynostemmae Pentaphylli extract can obviously increase the ACTH content of normal rat blood plasma.Plasma ACTH content under the stress state in increase electric shock rat vola.
(9) to the influence of digestive system:
1. hepatoprotective effect: Herb Gynostemmae Pentaphylli has the lipid peroxidation that suppresses impaired hepatic tissue to the carbon tetrachloride hepatic injury rat.Herb Gynostemmae Pentaphylli total glycosides 50mg/kg can suppress significantly because the rising of the caused serum glutamic pyruvic transminase vigor of carbon tetrachloride.
2. suppressing cholelithiasis generates: take Herb Gynostemmae Pentaphylli for a long time and can suppress experimental cholelithiasis formation, and have in the reduction blood and the effect of bile cholesterol.
3. antiulcer action: the cure rate of gastric ulcer is 46.79%, as takes medicine and extend to 15 days, can make cure rate be increased to 56.72%.
(10) other effect: Herb Gynostemmae Pentaphylli can be improved the scalp microcirculation, promotes lipid to discharge, and improves and the recovery cell viability, has black hair, hair growth promoting, beauty treatment skin, high honour appearance effect.Take Peptic Ulcers recovery from illness time of the old gastric ulcer patient of Herb Gynostemmae Pentaphylli total glycosides 0.5～1g/d for a long time, poliosis is also pitch-black gradually.Herb Gynostemmae Pentaphylli drinks or takes extractum 1g/d as tea, continuous March to 1 year, most of hair color recovery; Share with soyasaponins, each 20g/d * 5～15 month can make the hair that comes off regenerate.The hair pulling force of handling through Herb Gynostemmae Pentaphylli also obviously increases.Show antimutagenic effect is arranged.
(11) toxic and side effects:
1. acute toxicity: it is 48.94 ± 1.074g/kg that Herb Gynostemmae Pentaphylli extract is given the LD50 of NIH mouse stomach.The water extracted immersing paste is 2862.5 ± 338.0mg/kg to mouse peritoneal injection LD50.Herb Gynostemmae Pentaphylli total glycosides extractum mouse stomach LD50 is 4.5g/kg.The Herb Gynostemmae Pentaphylli total glycosides I and the general glycoside II lumbar injection LD50 in the different places of production are respectively 899.50～1051.32mg/kg and 1743.25～2049.11mg/kg.Herb Gynostemmae Pentaphylli total glycosides is 755mg/kg to the LD 50 of mouse peritoneal injection, oral avirulence.The LD50 of rats by intraperitoneal injection is 1.85g/kg.
2. long term toxicity: raise the food administration with the Herb Gynostemmae Pentaphylli of 4g/kg.d, continuous 90 days, all no abnormal change of the pathologic finding of the general state of rat, routine blood test, renal function, liver function, electrocardiogram and the heart, liver, kidney, testis.With one month rat of the continuous medicine feed of the Herb Gynostemmae Pentaphylli total glycosides of 8g/kg.d dosage, its general situation, body weight gain, food-intake, hematuria routine and histopathologic examination are all no abnormal.
3. specific toxicity: micronucleus test, Salmonella reversion test and testis sexual cell chromosome aberration assay test, sister chromatid exchange test (SCE) show that all Herb Gynostemmae Pentaphylli does not have mutagenic action.
The pharmacological action of Fructus Lycii
(1) anti-aging effects: the old volunteer of 60～85 years old no appreciable disease of 43 examples, chew clothes Fructus Lycii 50g every day, serveing on 10 is a course of treatment, finishes the 2nd day vein in back the course of treatment and gets blood, survey the index of the various immunologic functions of reflection, and some biochemical indicators of reflection old people functional status.The result shows that antalzyme activity, IgG, IgA and lymhocyte transformation rate significantly improve; Simultaneously, the increase of the back cAMP that takes medicine has the statistical significance of highly significant, and cGMP descends obviously, and cAMP/cGMP ratio is gone up, and remarkable rising is arranged after testosterone levels is taken medicine, and illustrates that Fructus Lycii can improve testosterone levels to a certain extent, plays strong effect.Fructus Lycii has nourishing liver and kidney, replenishing vital essence to improve eyesight, hard muscles and bones, strong body, clothes have delay aging, effect of prolonging life for a long time.
(2) estrogen sample effect: Fructus Lycii has significant uterus weight effect.Fructus Lycii, fruit stem all have the effect of significant promotion uterus weight to maturation or immaturity mice.
(3) to the promotion and the regulating action of immunologic function: the mouse gavaging lycium barbarum extracts, each 100% concentration 0.4ml, once a day, continuous three days, or its alcohol extract of an intramuscular injection 100% concentration 0.1ml, all can significantly improve the phagocytic activity of reticuloendothelial system to india ink.50% Fructus Lycii, fruit stem and leaf water extract 25g/kg, 0.5% lycium barbarum polysaccharide suspension 0.12g/kg give mouse stomach respectively, for three days on end or 5 days pneumoretroperitoneums injection broth bouillon 1ml, pneumoretroperitoneum was injected 2% chicken red blood cell 0.5ml in 22 hours, surveyed antalzyme activity and macrophage phagocytic percentage rate and phagocytic index in the serum after 40 minutes.The result shows that Fructus Lycii, fruit stem, leaf and lycium barbarum polysaccharide all can significantly improve phagocyte phagocytic percentage and phagocytic index, and Fructus Lycii, fruit stem and lycium barbarum polysaccharide can significantly improve the serum lysozyme vigor.
The Fructus Lycii polysaccharide can make the lymphopoiesis level be improved by the active influence to the mice spleen interleukin II, and can significantly improve the lymhocyte transformation rate of normal healthy people.23 examples are carried out the oral Fructus Lycii of malignant tumor patient (the 50g/ day of radiotherapy, serve on 10) the research tentative confirmation, Fructus Lycii can improve lymhocyte transformation rate and the macrophage phagocytic rate because of immunologic hypofunction patient due to radiotherapy or the malignant tumor, improves patient's immunologic function.
Give lycium barbarum polysaccharide 1～2mg/kg.d to Aged Mice, lumbar injection can make its spleen hemolysis plaque formation cell (PFC) be worth normal adult mice level in 7 days, and can make the 3H-TdR value of mixing improve about 10 times when giving lycium barbarum polysaccharide 5mg/kg.Super suitable dosage SRBC immunization (SOI) shows that the lycium barbarum polysaccharide of optimal dose has obvious regulating action to Aged Mice suppressor T lymphocyte (Ts), strengthens the Ts cell activity.Lycium barbarum polysaccharide can significantly increase the quantity and the vigor of macrophage C3b and Fc receptor, and can weaken the inhibitory action of hydrocortisone acetate to macrophage C3b and Fc receptor.Lycium barbarum polysaccharide is not only immunostimulant, and immunoregulation effect is arranged.
(4) blood fat reducing with protect the liver, lipotropy: Fructus Lycii can reduce the rat blood cholesterol, and the effect that has slight anti-rabbit experiment atherosclerosis to form confirms that Fructus Lycii, leaf all have protective effect to the hepatic injury that DL-second sulfur aminoacid causes.Folium Lycii can significantly reduce normal mouse liver fat.
(5) antitumaous effect: Fructus Lycii is 42% to the heavy suppression ratio of tumor of mice S180 solid tumor, and Fructus Lycii, leaf are to people's adenocarcinoma of stomach KATO-III cell, and Chinese wolfberry carpopodium, leaf all have obvious inhibitory action to the human cervical carcinoma Hela cell.Its mechanism of action shows that mainly suppressing cell DNA synthesizes, the interference cell division, and cell is grown ability drop again.Ultrastructural observation finds that Fructus Lycii, fruit stem and leaf are the most obvious to mitochondrial change to cancerous cell, thereby causes the cellular oxidation produce power to reduce, and influences duplicating with proteinic synthetic of DNA, and ability of cell proliferation is suppressed.
(6) to the influence of hemopoietic system: gavage continuous 10 days of 10% Fructus Lycii decoct 0.5ml every day, the normal mouse hemopoietic function is had facilitation, leukocyte count is increased.Fructus Lycii also has remarkable leukogenic effect to normal healthy people.Fructus Lycii can promote the recovery of hemopoietic function, and prevention and alleviate leukopenia prolongs the patient treatment phase and improves curative effect.
(7) to the effect of blood pressure: anesthesia rabbit record Blood pressure of carotid artery, the water extract 10ml/kg duodenal administration of 50% Fructus Lycii, fruit stem or leaf, or 50% Fructus Lycii, fruit stem water extract 2ml/kg, 25% Folium Lycii water extract 2ml/kg intravenous injection.The result shows that Fructus Lycii does not make significant difference to normal rabbits blood pressure, and Chinese wolfberry carpopodium and leaf have remarkable hypotensive effect, and the effect of Folium Lycii is better than Chinese wolfberry carpopodium again.Anesthetized cat record Blood pressure of carotid artery respectively with the intravenous injection of different component fructus lycii extracted solution, is observed administration front and back blood pressure.The result shows that the alcohol extract of Fructus Lycii and leaf has the hypotensive effect of short time, and water extract is not seen hypotensive effect.
(8) hypoglycemic activity: lycium barbarum extracts can cause the remarkable and persistent reduction of rat blood sugar, and carbohydrate tolerance raises, and thinks that its hypoglycemic activity is owing to wherein contain the derivant of guanidine.Early stage research reports that also Fructus Lycii has hypoglycemic activity.
(9) growth stimulation: betanin is biological stimulant, adds betanin 4～6kg in the feedstuff per ton and can make female chickling and male chickling body weight surpass matched group 12～13% and 17～18% respectively; Add betanin 8kg in the feedstuff per ton, can increase egg production, surpass matched group 24.3%.
(10) toxic and side effects: Fructus Lycii toxicity is very little.Betanin enters in the body and discharges with original shape, and the quiet notes of rat 2.4g/kg does not see toxic reaction, and respiratory arrest the whole body spasm appears, in mouse peritoneal injection 25g/kg within 10 minutes; The LD50 of Fructus Lycii water extract mouse subcutaneous injection is 8.32g/kg, and betanin is 18.74g/kg, shows that the former is twice many at toxicity than the latter.
The pharmacological action of Folium Camelliae sinensis
(1) central nervous system: Folium Camelliae sinensis and effective ingredient caffeine thereof have tangible excited central action, at first are cerebral cortex, and along with the increase of dosage, excited in succession oblongata influences spinal cord at last.
Caffeine is to the selective excitation of cerebral cortex, thus can eliminate sleepy, cheer up, lessen fatigue, and improve thinking activities.The active oral dose of the general excited cerebral cortex of caffeine (0.2～0.3g) does not influence normal bulbar center, only when subcutaneous injection heavy dose of (0.5g), and can excited respiratory center, vasomotor center and vagus nerve maincenter.When these maincenters were suppressed (anesthetics, infectious disease), caffeine then appreciable stimulating action can occur, thereby made exaggerated respiration, quickening, increased blood pressure, and heart rate is slightly slow in short-term, changes quickening very soon into.
(2) blood circulation: caffeine, theophylline directly act on heart, owing to can suppress the phosphodiesterase in the heart, thus the concentration of ring phosphorus one adenosine monophosphate (cAMP) is increased, thereby make cardiac muscle excited, mental and physical efforts occurring and strengthen, heart rate is accelerated.
To the effect of blood vessel, maincenter and tip double properties are arranged; Because can excited vasomotor center, thereby internal organs and cerebral vessels be shunk; But because direct blood vessel dilating smooth muscle still, and make skin, arteria coronaria, kidney vasodilation, so can be used for some hypertension.On the intensity of vasodilation and cardiotonic, theophylline is the most remarkable, and Ke Ke alkali takes second place, and caffeine is the most weak.
(3) diuresis: the caffeine class has significant diuresis, and its action intensity weakens successively by theophylline, caffeine, Ke Ke alkali.
(4) respiratory system: the contained theophylline of Folium Camelliae sinensis, caffeine, the Ke Ke alkali bronchial smooth muscle that can relax, effect is more obvious when bronchial smooth muscle is in spasticity, because theophylline can suppress PDE, the hydrolysis rate of Cyclic Adenosine monophosphate slows down, and increase its concentration in tissue, impel bronchial smooth muscle lax.The energy coronary artery dilator, but oxygen consumption increases simultaneously, and the existence of ethylene diamine can make bronchus effect loose and coronary dilation strengthen in the molecule.Theophylline can also reduce renal tubules to electrolytical heavy absorption, increases glomerular filtration rate and diuresis is arranged.Can treat bronchial asthma, cardiac edema, cardiac asthma etc.
(5) cholesterol reducing and fibrinolysis: tea tannin and Citrin, vitamin C, can alleviate atherosclerosis, reduce serum cholesterol, the ratio of cholesterol reducing and phosphide prevents that neutral fat from depositing in blood, liver.Impel Fibrinogen to be cracked into FDP rapidly and influence coagulation function, activate fibrinolysin unit-Wei lyase system, similar to the effect of urokinase.This anticoagulation aspect treatment coronary heart disease and atherosclerosis, is paid attention to for people.
(6) antibiotic: the Folium Camelliae sinensis decoct is the strongest to staphylococcus aureus and bacterium flexneri effect; Secondly be bacillus pyocyaneus, Bacillus typhi; And the sensitivity of escherichia coli, streptococcus and pneumobacillus is relatively poor.Also be used for urinary system infection, respiratory tract infection and suppurative wound.
(7) radioprotective: tea polyphenols, polysaccharide ester and Citrin, can alleviate radiocurable side effect, stop leukopenia, promote leukocyte increasing; Tea polyphenols can absorb 90Sr, makes 90Sr remove bone marrow.So there is the people that the Folium Camelliae sinensis exaggeration is " atom-age beverage ".
(8) tea tannin has convergence, antiinflammation: can be used for intractable ulcer; Contain micro-fluoride in the Folium Camelliae sinensis, the oral cavity of can sterilizing, caries prevention.
(9) smooth muscle: caffeine class, particularly theophylline, owing to suppress phosphodiesterase cAMP is increased, gastrointestinal tract, bronchial smooth muscle there are relaxation effect, so can be used for spasmolytic (biliary tract) and Zhichuan.
The effect of manganese and purposes (manganese sulfate)
Because of Herba Epimedii contains a large amount of manganese, so the pharmacological action of manganese is described below:
(1) manganese is arginase, RNA polymerase, and the composition of superoxide dismutase etc., and can activate some enzymes, and as: carboxylases etc. play an important role to endocrine regulation and immunologic function adjusting.
(2) manganese can make the neural blood vessel wall loose, reaches the blood vessel dilating effect, promotes the heart, brain, kidney and the blood supply of dimension blood vessel circulation, and the control heart, brain, renal vascular disease become and dimension vascular lesion and various nerve injury.
(3) manganese deficiency pancreas hypoplasia, insulin secretion reduces, and blood glucose increases, and causes diabetes.
(4) manganese deficiency can cause the skeleton pathological changes, osteodystrophy, and rheumatoid and rheumatic arthritis, osteoarticular injury, degeneration, the sclerotin paraplasm damages simultaneously for flesh and blood pipe and nerve, quickens osteopathia damage process and osteonecrosis etc.
(5) anemia appears in child's manganese deficiency, and monster appears in the anemia of pregnant woman, the congenital anomaly of skeletal bone pathological changes.
(6) manganese deficiency can cause the manganese enzymatic activity to reduce, the endocrine disturbance, and immunologic hypofunction also infects multiple infectious disease.
(7) manganese can impel people's slow down aging, and longevity has the effect of the free radical of removing
(8) consumption, feed ration 5～10mg.
Selenic effect and purposes
(1) selenium can be kept the penetrating function of cardiac cell membrane, mitochondrial membrane, lysosome membrane and Golgi membrane, can stablize cardiac muscle, brain, kidney, pancreas cell system integrity, postpone the generation of irreversible damage, can at protein synthesis and promote to rise in the cytothesis process to pretend very much usefulness, selenium can quicken the reparation of cardiovascular and cerebrovascularinfarction district cell, prolong anoxybiotic time-to-live of cardiac-cerebral ischemia and heart and brain automatic beats time, so, prevention and therapeutical effect are arranged to the cardio cerebrovascular affection that a variety of causes causes.
(2) lack selenium and can cause the heart, brain, vascular lesion, as: coronary heart disease, heart and brain pipe pipe arteriosclerosis, hypertension, deficiency myocardial blood supply, the heart power deficiency that contracts, myocardial infarction and cerebral infarction etc., selenium supplement can reverse and treat above-mentioned sick damage.
(3) lack selenium and can cause that osteoarthrosis produces pathological changes, as: Keshan disease, Kaschin-Beck disease, rheumatic and rheumatoid arthritis, osteoarticular injury caused.
(4) lack selenium area cancer general mortality rate and increase, increase selenium and take in, can reduce the generation of cancer.
(5) selenium can also vision enhancing, immune stimulatory globulin and production of antibodies.Strengthen the ability of body resistance disease, make each organ of body avoid infringement.
(6) selenium is the cofactor of paddy Guang peptide peroxidase, has the effect of removing free radical and peroxide, makes body avoid the peroxide infringement.Prolong life, life lengthening.
(7) cardio cerebrovascular affection that diabetes are caused has prevention and therapeutical effect.Scarce selenium can make lipid catabolism unusual; Bringing out ketoboidies produces---the diabetes ketosis, and ketoboidies ↑+---the bupropion metabolite product ↑---PH ↓---acidosis---diabetic ketoacidosis, selenium supplement can suppress diabetes ketosis and diabetes acidosis to albuminolysis.
(8) selenium consumption: Chinese's consumption 0.03-0.06mg/ day, international amount 0.06mg/ day, toxic dose 6.35umol.
The effect of zinc and purposes (zinc sulfate)
(1) it is synthetic that zinc participates in interior 80 plurality of enzymes of body, and pancreas, gonad, activity pituitary all have zinc to participate in.
(2) keep the normal development function of male's gland, zinc deficiency can make oligospermia, and impotence generandi forms male sterility.
(3) the human body barriers function can be kept and strengthen to zinc, performance defence infection effect.
(4) zinc and cardiovascular and cerebrovascular vessel have certain relation, and zinc and cadmium be mutual antagonism in biosystem, and oral zinc can replace cadmium in the arterial wall, and expansion ischemic region blood vessel.
(5) zinc has the promotion growth promoter, improves the sense of taste etc., growth retardation during zinc deficiency, and nervous function depletion can have conjunctivitis, stomatitis, glossitis, inappetence, chronic diarrhea etc.
(6) zinc counts for much to upgrowth and development of children, and the zinc deficiency visible volume is thin and weak, mental retardation etc.
(7) feed ration: 10～15mg/ day, maximum 120mg/ day.
The effect of chromium and purposes (chromium trichloride)
(1) trivalent chromium is a glucose tolerance factor: 1. human body lacks chromium and can cause carbohydrate tolerance impaired or develop into diabetes, hyperlipemia, atherosclerosis, growth promoter and stagnate and the lost of life, and chromium supplement can reverse above-mentioned phenomenon.2. form ternary complex between chromium insulin and the DNA mitochondrial DNA, promote that insulin plays a role---blood sugar lowering.3. chromium is normal glycometabolic necessary dimension secondary element, and chromium deficiency is relevant with type ii diabetes in the diet.4. trivalent chromium is relevant with the islets of langerhans activity, lacks the chromium insulin active and reduces---and cause diabetes, hyperlipemia, atherosclerosis occurs.
(2) chromium is to glycometabolic influence: high chromium raising rat and monkey, blood glucose, glycerol, three fat, cholesterol all significantly descend, blood glucose in diabetic rats descends 14～29%, the foreign data report: six routine diabetes patients, and hyperglycemia takes place to lose weight, movement disorder, peripheral neuropathy takes place, through supply every day chromium 250 μ g, 2 weeks of logotype, without insulin, suffer from carbohydrate tolerance and function of nervous system be restored.Thereafter, supply every day 20 μ g each, carbohydrate metabolism can be kept normally.Clinical trial certificate: chromium supplement or GTF (the carbohydrate tolerance factor) can improve diabetes patient's impaired glucose tolerance person's glucose tolerance, and lowering blood sugar and blood fat improves insulin sensitivity, has increased insulin bioactivity.After the old people of people to 76 routine impaired glucose tolerance arranged again, from diet, supply with chromium 200 μ g every day, to its sugar the anti-double blinding crossing research that carries out, three months is a course of treatment, carbohydrate tolerance sufferer as a result, chromium supplement carbohydrate tolerance therebetween obviously improves, and the comfort group does not have this effect.
(3) replenish and toxicological study in the chromium: 1. the foreign scholar replenishes the trivalent chromium of 100～200 μ g and obtains promising result with the beer yeast of inorganic form or GTF and the high chromium yeast of artificial culture to the people who lacks chromium.2. chromium has the necessarily property accumulated in vivo, and biological half-life is 27 days, and the toxicity of chromium and existence have very big relation, and the trivalent chromium chemical property is torpescence very, and toxicity is extremely low, and difficulty causes poisoning.3. clinical trial: trivalent chromium and chromium acetate difference 0.8mg/kg and 2.29mg/kg give mice, do not see toxicity.Chromium trichloride is oral to be 1870mg/kg to rat LD50, mouse peritoneal injection LD50 is 140mg/kg, having the human chromium trichloride that Mus is carried out i.v injection LD is 10mg/kg, more than like this zoopery be to be used for the treatment of anti-unusual tens thousand of times of diabetes and sugar, as seen very wide safety range is arranged therebetween.Have human to contain the drinking-water raising rat 1 year of trivalent chromium 25mg/L, do not see liver is arranged, the kidney aspect damages and toxicity, serve on three months with the water of 50～1000mg/L for cat, do not see toxic reaction.4. the mutagenic research of chromium trichloride: have only when heavy dose of Chlorizate chromium 29mg/kg mouse peritoneal is injected, certain teratogenesis is just arranged.For trivalent chromium whether carcinogenesis is arranged, do not appear in the newspapers as yet.Therefore only give oral trivalent chromium, safety range is quite wide.Replenish 50～200 μ g every day and can not cause toxic reaction.
(4) modern clinical practice: 250 μ g/ days of chromium trichloride serve on 1, change for 25 μ g/ days into after blood sugar urinary sugar is normal, and patient's carbohydrate tolerance and nerve are restored, and later 25 μ g/ day carbohydrate metabolism can be kept normally.
Below in conjunction with embodiment, the present invention will be further described.
The prescription of this Youth and longevity pellet consists of Herba Epimedii, Herb Gynostemmae Pentaphylli, Fructus Lycii, Folium Camelliae sinensis.
The preparation method of this Youth and longevity pellet is realized by following concrete reparation technology:
(1) Herba Epimedii selenium extraction process: (1) crude product Herba Epimedii selenium extraction process: the former medicine of crude product adds 8 times of amount 1% selenic acid liquid, kept 80 ℃ of reflux, extract, 3 hours, the leaching medicinal liquid is put in addition, residue adds 5 times of amount 1% selenic acid liquid again, pressing the Same Way operation extracted 2 hours, multiple leaching medicinal liquid and preceding liquid merge, and gather all selenic acid extracting solution, are condensed into than weighing 1.1～1.2, add 95% ethanol equivalent precipitated impurities, left standstill 12～14 hours, and extracted supernatant liquid filtering, precipitation is with 45% washing with alcohol 2～3 times, the solubility composition washes out in will precipitating, filtrate and washing liquid merge, and reclaim ethanol, and concentrating under reduced pressure is cooled to cream.Promptly get the thick extracted extract of Herba Epimedii selenium.(2) pure product Herba Epimedii selenium extraction process: the thick extracted extract of Herba Epimedii selenium adds 70%4 times of amount ethanol, and reflux 2 hours is filtered, and filtering residue adds 2 times of amount alcohol refluxs 1 hour again, filters, and reclaims ethanol, and 60 ℃ of dryings promptly get pure product Herba Epimedii selenium.(3) epimedium brevicornum polysaccharide selenium extraction process: Herba Epimedii is pulverized coarse powder and is added 5 times of amounts of 75% ethanol, 0.5% selenic acid (Ha2SeO4) reflux 2 hours, filter, extract 3 times with method, merging filtrate is condensed into cream, adds 95% ethanol with being stirred to precipitation, 12 hours after-filtration, reclaim ethanol, continuous 2 times, add 2 times of amounts of water, with DEAE cellulose (phosphate type) column chromatography purification, get pure product epimedium brevicornum polysaccharide selenium.
(2) Herb Gynostemmae Pentaphylli total glycosides chromium extraction process: natural Herb Gynostemmae Pentaphylli is a raw material, filter with 38 times of water gaging lixiviates of 1% chromic oxide gel Cr (OH), extract 3 times continuously, merging filtrate, adopt the resin adsorption method production technology, select for use the D101 type adsorbent resin of function admirable and nontoxic ethanol to take off agent, process route is simplified as washing liquid, economic security, and product purity and recovery rate height.Other has the macro-reticular adsorbing resin method to extract gypenoside, adopts 1%Cr (OH) 3 floodings, D type non-polar resin adsorption and purification, and the method for activated carbon decolorizing, total Saponin yield is about 2.15%.1. extract with 50% ethanol, the medicinal liquid cocnentration factor weighs 1.14 before the precipitate with ethanol, and determining alcohol is 70%, adds 3 times of water gagings of medicinal liquid during water precipitating, the oral liquid clarity of preparation is taken a turn for the better, and gypenoside content is also higher.2. about medicinal liquid degree of being condensed into before the precipitate with ethanol, once there was report medicinal liquid ratio to weigh 1.25, PH is 4.20, and mixing time is 1.5 hours, and the stability of Oral of making like this is good, and quality meets the requirements, and said preparation effect duration is 4.69a.
(3) Fructus Lycii extracts Folium hydrangeae strigosae alkali, lycium barbarum polysaccharide zinc extraction process: get former medicine by the seepage method, with moistening 4 hours of 1% zinc sulfate, 45% ethanol, with 1% zinc sulfate, 45% ethanol is solvent dipping 24 hours, speed seepage with per minute 50ml/5kg, when making transudate reach 7 times of amounts of crude drug, promptly stop seepage, collection is got sepage, reclaim ethanol, and thickening filtration.Be adjusted to every ml and contain total amount 150mg, add 0.25% benzoic acid again, 0.1% nipalgin is anticorrosion, leaves standstill 8～10 days, gets the supernatant and filters, and, is dried into dry extract for 60 ℃ that is.
(4) Folium Camelliae sinensis extracts theophylline and caffeine technology: Folium Camelliae sinensis adds 8 times of amount 1% sulphuric acid liquid and soaks seepage after 14 hours, continuous 3 times, merging filtrate is concentrated into original volume 1/5 and filters, and filtrate transfers to PH7 with ammonium hydroxide (NH4OH), add 95% ethanol equivalent precipitated impurities, left standstill 12 hours, and extracted supernatant liquid filtering out, precipitation is with 45% washing with alcohol 2～3 times, be condensed into cream, promptly get theophylline and caffeine crystallization.
(5) preparation process of Youth and longevity pellet:
Herba Epimedii selenium 50mg/kg/ day, epimedium brevicornum polysaccharide selenium 20mg/kg/ day; . Herb Gynostemmae Pentaphylli total glycosides chromium 2.5mg/kg/ day; Fructus Lycii consumption extract Fructus Lycii is extracted betanin 1.5mg/kg/ day, lycium barbarum polysaccharide zinc 1.5mg/kg/ day; Folium Camelliae sinensis extract theophylline 8mg/kg/ day, caffeine 4mg/kg/ day; Altogether 4375mg/50kg/ day, be divided into 10 large capsules, each capsule contains 437.5mg.
Pharmacokinetics:
Trace manganese is in the dynamic (dynamical) research of generation of Sanguis Leporis seu oryctolagi Chinese medicine in the Herba Epimedii, with the Catalytic-Kinetic Analysis is monitoring means, utilize the catalytic action of divalent manganesetion to Potassium metaperiodate .-rhodamine B reaction system, use the Phen activator, trace manganese has carried out fluoremetry in the blood to exempting from, curve when having drawn medicine, and the result is through MICROCOMPUTER PROCESSING, physiological disposition meets two Room open models, and has obtained serial pharmacokinetic parameter.The Herb Gynostemmae Pentaphylli oral absorption is fast, and distribution is wide, drains slowly, discharges 10% in 24 hours, may be due to liver sausage circulates.Theophylline promptly excreted in general 6～10 hours.Six, indication: be applicable to geromorphism, worn with age, neurasthenia, fatigue; In, old man, women's hypofunction or obstacle etc.; Cardiovascular and cerebrovascular disease, myocardial infarction, cerebral infarction, heart failure; In the control, the generation of old osteitis, bone regeneration, osteonecrosis; In the control, the generation of old hepatitis, gastric ulcer; The generation of control bronchitis, asthma, emphysema, pulmonary heart disease; Calmness, hypnosis, hypermnesis are arranged, control senile insomnia, lumbago and skelalgia, brain atrophy, alzheimer disease, prevention and treatment tumor.Long this medicine of clothes prevents and treats the generation of various pathological changes, recovers one's youthful vigour, and youth length exists life lengthening.
Claims (2)
1. a Youth and longevity pellet is characterised in that its prescription consists of Herba Epimedii, Herb Gynostemmae Pentaphylli, Fructus Lycii, Folium Camelliae sinensis.
2. the preparation method of a Youth and longevity pellet is characterised in that concrete reparation technology is as follows:
(1) Herba Epimedii selenium extraction process:
(1) crude product Herba Epimedii selenium extraction process: the former medicine of crude product adds 8 times of amount 1% selenic acid liquid, kept 80 ℃ of reflux, extract, 3 hours, the leaching medicinal liquid is put in addition, residue adds 5 times of amount 1% selenic acid liquid again, pressing the Same Way operation extracted 2 hours, multiple leaching medicinal liquid and preceding liquid merge, and gather all selenic acid extracting solution, are condensed into than weighing 1.1～1.2, add 95% ethanol equivalent precipitated impurities, left standstill 12～14 hours, and extracted supernatant liquid filtering, precipitation is with 45% washing with alcohol 2～3 times, the solubility composition washes out in will precipitating, filtrate and washing liquid merge, and reclaim ethanol, and concentrating under reduced pressure is cooled to cream; Promptly get the thick extracted extract of Herba Epimedii selenium;
(2) pure product Herba Epimedii selenium extraction process: the thick extracted extract of Herba Epimedii selenium adds 70%4 times of amount ethanol, and reflux 2 hours is filtered, and filtering residue adds 2 times of amount alcohol refluxs 1 hour again, filters, and reclaims ethanol, and 60 ℃ of dryings promptly get pure product Herba Epimedii selenium;
(3) epimedium brevicornum polysaccharide selenium extraction process:
Herba Epimedii is pulverized coarse powder and is added 5 times of amounts of 75% ethanol, 0.5% selenic acid (Ha2SeO4) reflux 2 hours, filter, extract 3 times with method, merging filtrate is condensed into cream, adds 95% ethanol with being stirred to precipitation, 12 hours after-filtration, reclaim ethanol, continuous 2 times, add 2 times of amounts of water, with DEAE cellulose (phosphate type) column chromatography purification, get pure product epimedium brevicornum polysaccharide selenium;
(2) Herb Gynostemmae Pentaphylli total glycosides chromium extraction process:
Natural Herb Gynostemmae Pentaphylli is a raw material, filter with 38 times of water gaging lixiviates of 1% chromic oxide gel Cr (OH), extract 3 times continuously, merging filtrate adopts the resin adsorption method production technology, selects for use D101 type adsorbent resin and nontoxic ethanol to take off agent as washing liquid, other has the macro-reticular adsorbing resin method to extract gypenoside, adopt 1%Cr (OH) 3 floodings, D type non-polar resin adsorption and purification, activated carbon decolorizing;
1. extract with 50% ethanol, the medicinal liquid cocnentration factor weighs 1.14 before the precipitate with ethanol, and determining alcohol is 70%, adds 3 times of water gagings of medicinal liquid during water precipitating, the oral liquid clarity of preparation is taken a turn for the better, and gypenoside content is higher;
2. medicinal liquid degree of being condensed into before the precipitate with ethanol, the medicinal liquid ratio weighs 1.25, and PH is 4.20, and mixing time is 1.5 hours;
(3) Fructus Lycii extracts Folium hydrangeae strigosae alkali, lycium barbarum polysaccharide zinc extraction process:
Getting former medicine by the seepage method, with moistening 4 hours of 1% zinc sulfate, 45% ethanol, is solvent dipping 24 hours with 1% zinc sulfate, 45% ethanol, with the speed seepage of per minute 50ml/5kg, when making transudate reach 7 times of amounts of crude drug, promptly stop seepage, collection is got sepage, reclaims ethanol, and thickening filtration; Be adjusted to every ml and contain total amount 150mg, add 0.25% benzoic acid again, 0.1% nipalgin is anticorrosion, leaves standstill 8～10 days, gets the supernatant and filters, and, is dried into dry extract for 60 ℃ that is;
(4) Folium Camelliae sinensis extracts theophylline and caffeine technology:
Folium Camelliae sinensis adds 8 times of amount 1% sulphuric acid liquid and soaks seepage after 14 hours, continuous 3 times, merging filtrate is concentrated into original volume 1/5 and filters, and filtrate transfers to PH7 with ammonium hydroxide (NH4OH), add 95% ethanol equivalent precipitated impurities, left standstill 12 hours, and extracted supernatant liquid filtering out, precipitation is with 45% washing with alcohol 2～3 times, be condensed into cream, promptly get theophylline and caffeine crystallization;
(5) preparation process of Youth and longevity pellet:
Herba Epimedii selenium 50mg/kg/ day, epimedium brevicornum polysaccharide selenium 20mg/kg/ day; Herb Gynostemmae Pentaphylli total glycosides chromium 2.5mg/kg/ day; Fructus Lycii consumption extract Fructus Lycii is extracted betanin 1.5mg/kg/ day, lycium barbarum polysaccharide zinc 1.5mg/kg/ day; Folium Camelliae sinensis extract theophylline 8mg/kg/ day, caffeine 4mg/kg/ day; Altogether 4375mg/50kg/ day, be divided into 10 large capsules, each capsule contains 437.5mg.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101375972B (en) * | 2008-09-18 | 2011-08-17 | 中国科学院武汉植物园 | Health oral liquid and its preparation |
| CN104799271A (en) * | 2014-01-26 | 2015-07-29 | 段胜保 | Sexual function health product |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1099642A (en) * | 1994-02-19 | 1995-03-08 | 吴金中 | Making method of wine for nourishing and strengthening vital function |
| CN1140023A (en) * | 1995-07-10 | 1997-01-15 | 蔡英剑 | Health bagged trace element tea and its preparing process |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101375972B (en) * | 2008-09-18 | 2011-08-17 | 中国科学院武汉植物园 | Health oral liquid and its preparation |
| CN104799271A (en) * | 2014-01-26 | 2015-07-29 | 段胜保 | Sexual function health product |
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