CN1268353A - Preparation method of freeze-dried common carp swimmer - Google Patents

Abstract

A preparation method of freeze-dried carp air bladder for patching hard brain membrane is disclosed. Said method includes: kill the live carp, take out its air bladder, immerse it in phosphate equilibrium solution with isoosmotic pressure, rinse with normal saline, fix in 0.3% glutaraldehyde solution, immerse in 1 N NaOH solution and 10% H2O2 solution, and after freeze dried, it is sealed and packed for use.

Description

A kind of preparation method of freeze-dried common carp swimmer

The invention belongs to a kind of preparation method that is applied to the freeze-dried common carp swimmer of duramater reparation.

Kind surplus the material bibliographical information that is applied to duramater reparation has at present reached 40 mainly comprises a, as metal foil films such as native gold, native silver, and this kind material is because of the complication abandoned already of severe postoperative; B, synthetic organic material, it is clinical to be with terylene silicone rubber that the synthetic material of representative once was widely used in, but easily causes postoperative complication such as tardy property intracranial hematoma to occur; C, natural biological film class such as people's lyophilizing cerebral dura mater are because of costing an arm and a leg, and the source is difficult, and the report of the lyophilizing cerebral dura mater postoperative infection spongiform disease toxicity encephalopathy of should choosing is arranged in recent years repeatly, and the use of people's lyophilizing meninges is restricted.

The preparation method of new material-lyophilizing Air Bladder pseudosciaenae seu Acipenser that the object of the present invention is to provide a kind of duramater reparation is to overcome the deficiency of prior art.

Overall technology design of the present invention is:

A kind of preparation method of freeze-dried common carp swimmer, it comprises following processing step:

A, the live carp of getting every tail 1-2kg kill the back and take out Air Bladder pseudosciaenae seu Acipenser, and Air Bladder pseudosciaenae seu Acipenser is placed the phosphate balance solution of isoosmotic PH=7.4, remove the tissues such as fat, blood vessel, nerve on Air Bladder pseudosciaenae seu Acipenser surface;

B, the Air Bladder pseudosciaenae seu Acipenser after the above-mentioned processing inserted after normal saline flushing 20-30 minute in 0.3% the glutaraldehyde solution and fix 14 days;

C, the Air Bladder pseudosciaenae seu Acipenser that fixes is dipped in the NaOH solution of 1N after 1 hour, was dipped in again in 10% the hydrogenperoxide steam generator 24 hours, the Air Bladder pseudosciaenae seu Acipenser after disposing is carried out lyophilization handle the back sealing and preserve.

Other technologies feature of the present invention also has:

Process conditions are temperature-40 ℃ in the lyophilization processing procedure, air pressure 0.013KPa.

Below in conjunction with embodiment the present invention is further described:

Fish in the present embodiment is selected live carp for use, live carp is killed, taking out rapidly Air Bladder pseudosciaenae seu Acipenser, to place PH be 7.4 isosmoticity phosphate balance solution, wipe fat, blood vessel, the nervous tissue that removes the Air Bladder pseudosciaenae seu Acipenser surface with gauze, insert after 20 minutes with normal saline flushing to soak in 0.3% the glutaraldehyde solution and fix 14 days, again the Air Bladder pseudosciaenae seu Acipenser after the above-mentioned processing is dipped in the NaOH solution of 1.0N and was placed on 10% H in 1 hour ₂O ₂In the solution 24 hours, to carry out lyophilization then and handle (temperature-40 ℃, air pressure 0.013KPa), sealing places the double-layer polyethylene plastic bag, and is standby with oxirane disinfection.

The obtained technological progress of the present invention is:

These freeze-dried common carp swimmer raw material sources are extensive, and the preparation method is simple, are easy to sterilization and store, and product has Stable any biological inert is difficult for causing the Acute and chronic inflammation reaction, and anti-seam is good, with people's endocranium Institutional framework is similar, can impel dura mater regeneration, prevents the adhesion of meninx brain, its peripheral nerve tissue is not produced And damage, do not spread disease no carcinogenesis.

Claims (2)

1, a kind of preparation method of freeze-dried common carp swimmer is characterized in that it comprises following processing step:

A, the live carp of getting every tail 1-2kg kill the back and take out Air Bladder pseudosciaenae seu Acipenser, and Air Bladder pseudosciaenae seu Acipenser is placed the phosphate balance solution of isoosmotic PH=7.4, remove the tissues such as fat, blood vessel, nerve on Air Bladder pseudosciaenae seu Acipenser surface;

B, the Air Bladder pseudosciaenae seu Acipenser after the above-mentioned processing inserted after normal saline flushing 20-30 minute in 0.3% the glutaraldehyde solution and fix 14 days;

C, the Air Bladder pseudosciaenae seu Acipenser that fixes is dipped in the NaOH solution of 1N after 1 hour, was dipped in again in 10% the hydrogenperoxide steam generator 24 hours, the Air Bladder pseudosciaenae seu Acipenser after disposing is carried out lyophilization handle the back sealing and preserve.

2, the preparation method of freeze-dried common carp swimmer according to claim 1 is characterized in that in the lyophilization processing procedure that process conditions are temperature-40 ℃, air pressure 0.013KPa.